AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84731

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84731.t1 MTR_8g027140 97.813 1006 22 0 1 1006 1 1006 0.0 2009
MS.gene84731.t1 MTR_7g117890 70.547 1005 292 2 2 1006 1 1001 0.0 1429
MS.gene84731.t1 MTR_1g076400 28.332 1013 676 20 11 996 8 997 6.33e-106 354
MS.gene84731.t1 MTR_3g019360 34.337 664 420 6 345 1006 178 827 2.36e-102 340
MS.gene84731.t1 MTR_3g115670 27.960 794 518 24 257 1006 27 810 1.65e-71 254
MS.gene84731.t1 MTR_4g063800 24.329 596 394 18 37 590 27 607 2.69e-20 97.1
MS.gene84731.t1 MTR_7g078330 25.052 483 318 13 81 555 98 544 5.58e-20 95.9
MS.gene84731.t1 MTR_7g059405 25.414 362 223 8 261 601 266 601 5.83e-19 92.8
MS.gene84731.t1 MTR_4g485520 24.451 364 229 8 261 612 107 436 2.41e-16 83.6
MS.gene84731.t1 MTR_2g018010 23.355 471 302 10 89 510 112 572 4.18e-16 83.6
MS.gene84731.t1 MTR_4g485520 24.451 364 229 8 261 612 275 604 9.39e-16 82.4
MS.gene84731.t1 MTR_7g053260 26.471 340 213 12 263 595 82 391 1.25e-15 81.3
MS.gene84731.t1 MTR_8g077205 23.209 349 214 9 23 325 1 341 7.36e-15 79.7
MS.gene84731.t1 MTR_3g466220 25.294 340 201 13 19 338 6 312 6.96e-14 76.6
MS.gene84731.t1 MTR_5g077510 25.000 232 142 7 263 467 39 265 3.61e-13 73.6
MS.gene84731.t1 MTR_4g028960 22.676 441 272 16 88 478 112 533 3.98e-13 73.9
MS.gene84731.t1 MTR_3g466220 31.351 185 113 5 156 338 119 291 4.02e-13 74.3
MS.gene84731.t1 MTR_3g095730 24.274 379 204 13 263 602 17 351 7.53e-13 72.4
MS.gene84731.t1 MTR_5g034440 21.957 419 291 11 93 498 109 504 1.78e-12 72.0
MS.gene84731.t1 MTR_4g107010 22.998 487 324 16 93 541 111 584 2.54e-12 71.6
MS.gene84731.t1 MTR_3g008280 32.335 167 89 5 174 328 645 799 3.57e-12 71.2
MS.gene84731.t1 MTR_4g091880 34.783 92 49 2 261 351 7 88 3.61e-12 69.3
MS.gene84731.t1 MTR_3g008280 32.335 167 89 5 174 328 653 807 3.65e-12 71.2
MS.gene84731.t1 MTR_4g091880 34.783 92 49 2 261 351 7 88 3.73e-12 69.7
MS.gene84731.t1 MTR_2g011140 24.302 358 242 9 260 596 16 365 5.37e-12 69.3
MS.gene84731.t1 MTR_1g094215 27.946 297 175 8 263 550 68 334 1.04e-11 68.9
MS.gene84731.t1 MTR_8g092870 43.077 65 37 0 263 327 14 78 1.13e-11 68.6
MS.gene84731.t1 MTR_3g096370 42.857 63 36 0 263 325 14 76 2.18e-11 67.4
MS.gene84731.t1 MTR_1g094215 27.645 293 181 7 263 550 251 517 3.55e-11 67.8
MS.gene84731.t1 MTR_3g065080 39.726 73 44 0 262 334 39 111 3.68e-11 67.0
MS.gene84731.t1 MTR_8g077205 24.889 225 134 5 136 325 5 229 3.75e-11 67.4
MS.gene84731.t1 MTR_8g103227 28.467 137 94 3 194 329 333 466 4.15e-11 67.8
MS.gene84731.t1 MTR_1g017770 32.857 140 84 3 263 402 49 178 4.23e-11 66.6
MS.gene84731.t1 MTR_7g005940 46.269 67 35 1 261 326 597 663 4.73e-11 67.8
MS.gene84731.t1 MTR_7g005940 46.269 67 35 1 261 326 597 663 5.13e-11 67.4
MS.gene84731.t1 MTR_4g085720 39.474 76 39 1 257 325 5 80 6.17e-11 66.2
MS.gene84731.t1 MTR_3g078160 26.667 225 148 5 263 487 279 486 6.55e-11 67.0
MS.gene84731.t1 MTR_3g078160 26.667 225 148 5 263 487 279 486 6.68e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84731.t1 AT1G76390 28.662 785 517 25 261 1006 27 807 7.84e-62 226
MS.gene84731.t1 AT1G76390 28.662 785 517 25 261 1006 27 807 7.84e-62 226
MS.gene84731.t1 AT1G20780 27.949 780 521 22 261 1006 25 797 1.18e-57 214
MS.gene84731.t1 AT1G20780 27.949 780 521 22 261 1006 25 797 1.18e-57 214
MS.gene84731.t1 AT1G20780 27.949 780 521 22 261 1006 25 797 1.18e-57 214
MS.gene84731.t1 AT1G68940 24.524 893 595 28 170 1006 216 1085 1.43e-51 197
MS.gene84731.t1 AT1G68940 25.246 812 543 25 170 935 163 956 3.04e-50 193
MS.gene84731.t1 AT1G68940 25.246 812 543 25 170 935 216 1009 3.39e-50 193
MS.gene84731.t1 AT5G42340 25.912 521 304 18 16 492 54 536 2.58e-21 100
MS.gene84731.t1 AT1G29340 24.411 467 300 10 89 509 124 583 1.88e-19 94.7
MS.gene84731.t1 AT3G46510 22.973 518 335 13 13 486 6 503 2.57e-19 94.0
MS.gene84731.t1 AT1G60190 23.577 492 327 14 13 465 27 508 4.05e-18 90.1
MS.gene84731.t1 AT5G67340 22.735 585 373 15 50 591 48 596 4.50e-18 90.1
MS.gene84731.t1 AT5G67340 22.735 585 373 15 50 591 48 596 4.50e-18 90.1
MS.gene84731.t1 AT1G71020 25.824 364 226 11 261 612 97 428 1.40e-17 87.4
MS.gene84731.t1 AT1G71020 25.824 364 226 11 261 612 245 576 3.72e-17 87.0
MS.gene84731.t1 AT1G23030 26.361 349 231 7 263 590 245 588 1.55e-16 84.7
MS.gene84731.t1 AT1G24330 22.712 612 345 17 20 512 3 605 1.65e-16 85.1
MS.gene84731.t1 AT1G24330 22.712 612 345 17 20 512 3 605 1.65e-16 85.1
MS.gene84731.t1 AT2G28830 29.433 282 170 7 256 510 181 460 7.28e-16 82.8
MS.gene84731.t1 AT5G01830 22.976 457 295 16 88 505 109 547 5.37e-15 80.1
MS.gene84731.t1 AT1G67530 24.709 344 211 10 25 329 8 342 1.05e-14 79.0
MS.gene84731.t1 AT1G67530 24.709 344 211 10 25 329 8 342 1.99e-14 78.6
MS.gene84731.t1 AT1G67530 24.709 344 211 10 25 329 8 342 1.99e-14 78.6
MS.gene84731.t1 AT1G67530 24.709 344 211 10 25 329 8 342 1.99e-14 78.6
MS.gene84731.t1 AT3G54790 25.279 269 160 10 91 348 86 324 2.71e-13 74.7
MS.gene84731.t1 AT3G54790 25.279 269 160 10 91 348 86 324 2.71e-13 74.7
MS.gene84731.t1 AT3G54790 25.279 269 160 10 91 348 50 288 4.65e-13 73.9
MS.gene84731.t1 AT3G07360 23.971 413 231 14 187 587 4 345 6.55e-13 72.8
MS.gene84731.t1 AT1G49780 42.105 76 37 1 257 325 5 80 7.69e-13 72.4
MS.gene84731.t1 AT4G36550 45.070 71 39 0 261 331 221 291 2.02e-12 72.0
MS.gene84731.t1 AT1G10560 24.854 342 226 10 14 332 28 361 3.48e-12 71.2
MS.gene84731.t1 AT5G51270 37.615 109 54 3 223 329 708 804 1.80e-11 68.9
MS.gene84731.t1 AT5G51270 37.615 109 54 3 223 329 708 804 1.80e-11 68.9
MS.gene84731.t1 AT3G61390 42.647 68 39 0 258 325 277 344 3.82e-11 66.6
MS.gene84731.t1 AT2G23140 29.143 175 111 4 156 329 141 303 4.08e-11 67.8
MS.gene84731.t1 AT2G23140 27.604 192 138 1 401 591 527 718 7.74e-11 67.0
MS.gene84731.t1 AT2G23140 29.143 175 111 4 156 329 138 300 4.25e-11 67.8
MS.gene84731.t1 AT2G23140 27.604 192 138 1 401 591 524 715 8.13e-11 66.6
MS.gene84731.t1 AT2G23140 29.143 175 111 4 156 329 138 300 4.25e-11 67.8
MS.gene84731.t1 AT2G23140 27.604 192 138 1 401 591 524 715 8.13e-11 66.6
MS.gene84731.t1 AT3G61390 42.647 68 39 0 258 325 352 419 5.49e-11 66.6
MS.gene84731.t1 AT5G09800 41.538 65 38 0 263 327 15 79 9.65e-11 65.5

Find 157 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCGCGCCAAATCATATT+TGG 0.186259 8.3:+67185149 None:intergenic
TGAGTTATCTGATCAATTTC+TGG 0.187668 8.3:+67183576 None:intergenic
TAAGGAACTTAGCTCTTATT+TGG 0.210368 8.3:-67186442 MS.gene84731:CDS
TATGGAGAAAGCTGGATTTA+AGG 0.218387 8.3:-67186133 MS.gene84731:CDS
GCATACACACATCAAGTTTA+TGG 0.223246 8.3:-67184493 MS.gene84731:CDS
CAAGTTCTTATACAAATTCT+TGG 0.261077 8.3:-67185430 MS.gene84731:CDS
TTTAATTTCTTCCAATATTC+CGG 0.265541 8.3:+67186170 None:intergenic
TGGAGTCTGTGAGGCTTCTT+TGG 0.269871 8.3:-67183121 MS.gene84731:CDS
TCTATCTTTCCATTCTTCTA+TGG 0.273578 8.3:+67185600 None:intergenic
GTTCTTACATAATGATCTTC+AGG 0.295740 8.3:-67184630 MS.gene84731:CDS
ACTTTATAGCACTTGCTCTT+AGG 0.295870 8.3:+67183745 None:intergenic
AATATTGGAAGAAATTAAAA+AGG 0.297339 8.3:-67186166 MS.gene84731:CDS
TTCTCAGCTCCGAGCGAAAT+TGG 0.307554 8.3:-67183246 MS.gene84731:CDS
AAGTCCATTTCTGTTTAAAC+TGG 0.309308 8.3:+67184571 None:intergenic
TGAATTATCCAAATATGATT+TGG 0.309894 8.3:-67185157 MS.gene84731:CDS
GGTTGTGTCACACTGATTAA+AGG 0.311802 8.3:+67184362 None:intergenic
TTCCTCAATTCTTTCAAAAT+CGG 0.318543 8.3:+67186408 None:intergenic
TCAAGGTTGCATACTTCTAT+TGG 0.318559 8.3:-67185112 MS.gene84731:CDS
TTTGCGAAACGGCAATCTTT+AGG 0.329672 8.3:-67185826 MS.gene84731:CDS
GGATCAAATCATTGCTTTGT+TGG 0.331319 8.3:-67185896 MS.gene84731:CDS
AAGTACACCACCGTCAAATA+AGG 0.337044 8.3:+67184670 None:intergenic
TGCTCGTGTTGATAAGAAAA+AGG 0.337238 8.3:-67185935 MS.gene84731:CDS
TGCTGCAAGTTGCATAATTA+TGG 0.338187 8.3:+67184457 None:intergenic
TCACACAACCTAACCAATTC+TGG 0.340227 8.3:+67183783 None:intergenic
TTAGTGCAGTTGCTTGAAAT+TGG 0.341628 8.3:-67183375 MS.gene84731:CDS
AATCTTGGAGAATCTGATTA+TGG 0.345438 8.3:-67183141 MS.gene84731:CDS
GGCCAATACATTAGCCGAAA+TGG 0.346085 8.3:-67184717 MS.gene84731:CDS
TTTCTTAGTTCTACCTCTCT+TGG 0.346307 8.3:-67183009 MS.gene84731:CDS
ATCTTTAGGTACTCGAATCT+TGG 0.353618 8.3:-67185812 MS.gene84731:CDS
TTTATTTGTTAATGAATTGT+AGG 0.364173 8.3:-67186278 MS.gene84731:CDS
GTTCGAGAAACCGTGATATA+AGG 0.366447 8.3:-67185408 MS.gene84731:CDS
TTCCATTTCGGCTAATGTAT+TGG 0.367156 8.3:+67184715 None:intergenic
TCTCAAATCATAGAAACTAT+TGG 0.367335 8.3:-67186513 MS.gene84731:CDS
TGAACATATTTATAAATGAT+TGG 0.370188 8.3:+67183528 None:intergenic
TGACTGACACAAGGCGTAAA+AGG 0.371641 8.3:+67184313 None:intergenic
CTTCTTCCTTTAGCCGCATC+AGG 0.373069 8.3:-67186201 MS.gene84731:CDS
TATTTCCTCTTCATCATATG+AGG 0.376147 8.3:+67183629 None:intergenic
AGGTCTTTCAGCCGGAATAT+TGG 0.377612 8.3:-67186181 MS.gene84731:CDS
CTTCATCATATGAGGATTGT+AGG 0.381313 8.3:+67183637 None:intergenic
GGAATTAACTGACCACAATA+AGG 0.387274 8.3:-67184696 MS.gene84731:CDS
AGACCGATTCCGAAGCAAAA+AGG 0.388092 8.3:-67183282 MS.gene84731:CDS
TTCTTAGTTCTACCTCTCTT+GGG 0.394620 8.3:-67183008 MS.gene84731:CDS
GAAAATCGAGTCAGGGATAA+GGG 0.403660 8.3:-67186082 MS.gene84731:CDS
CATACACACATCAAGTTTAT+GGG 0.404356 8.3:-67184492 MS.gene84731:CDS
AGATGATGTAAAGATGATTA+TGG 0.405222 8.3:-67184738 MS.gene84731:CDS
CTATCCAGTTTAAACAGAAA+TGG 0.406424 8.3:-67184575 MS.gene84731:CDS
CTAGTAACAAAACCGGCGTT+TGG 0.413747 8.3:+67184414 None:intergenic
GTGGTCAGTTAATTCCATTT+CGG 0.414212 8.3:+67184703 None:intergenic
CAGCTATACCAGAATTGGTT+AGG 0.419391 8.3:-67183791 MS.gene84731:CDS
TGATCTGATGAAGTTTCAAC+AGG 0.425758 8.3:+67185739 None:intergenic
AGCTGCAAACCTACTTGCTC+TGG 0.425914 8.3:-67186322 MS.gene84731:CDS
TCTGATCAATTTCTGGAAGA+TGG 0.428541 8.3:+67183583 None:intergenic
GTGCTCAGCTATACCAGAAT+TGG 0.431004 8.3:-67183796 MS.gene84731:CDS
CCATTCTCTTGGACGACGTC+AGG 0.431093 8.3:-67185211 MS.gene84731:CDS
AGAAAATCGAGTCAGGGATA+AGG 0.432371 8.3:-67186083 MS.gene84731:CDS
TATTTCAGTTTCCTTTCGTT+CGG 0.443580 8.3:+67185850 None:intergenic
GGACCACTCACAAGAAATCT+TGG 0.446995 8.3:-67183156 MS.gene84731:CDS
TCCTATCACACATGATGTTA+TGG 0.452740 8.3:-67185767 MS.gene84731:CDS
TTCGCGATTCATAATATCAA+TGG 0.454754 8.3:+67186350 None:intergenic
AAAGGCTGAAGCTATGCAAA+TGG 0.456067 8.3:-67185917 MS.gene84731:CDS
AATCTCATTGCTAACGCTGT+TGG 0.459825 8.3:-67186021 MS.gene84731:CDS
AGTCTCTACATGCTTTAGAA+AGG 0.467873 8.3:-67182975 MS.gene84731:CDS
ATCAACATTCTCTACTATGA+TGG 0.468562 8.3:+67183689 None:intergenic
GAATCGATAGTCCATTCTCT+TGG 0.470166 8.3:-67185222 MS.gene84731:CDS
ACCATAACATCATGTGTGAT+AGG 0.470871 8.3:+67185766 None:intergenic
GAAATTGGCTGTAAAGTTCA+TGG 0.472815 8.3:-67183231 MS.gene84731:CDS
GTTGTGTCACACTGATTAAA+GGG 0.473597 8.3:+67184363 None:intergenic
CCTGACGTCGTCCAAGAGAA+TGG 0.475497 8.3:+67185211 None:intergenic
TGGATTTAAGGCTGCGGTAG+CGG 0.475565 8.3:-67186121 MS.gene84731:CDS
CTCAAAACCGATGTATCTAA+AGG 0.478675 8.3:+67185646 None:intergenic
ATTGTAGGTCGATTGTAAAT+CGG 0.479909 8.3:-67186263 MS.gene84731:CDS
GAACACATTGGAAAAGTTCA+AGG 0.482125 8.3:-67185129 MS.gene84731:CDS
TCACACTGATTAAAGGGAAA+AGG 0.486884 8.3:+67184369 None:intergenic
TGTGATAATATGGAGAAAGC+TGG 0.488266 8.3:-67186141 MS.gene84731:CDS
TAAAGATGCAACTGAAGTAT+TGG 0.488860 8.3:-67185055 MS.gene84731:CDS
GCAGAGTTCAAACAGTTGTA+TGG 0.490896 8.3:-67182940 MS.gene84731:CDS
AGAGGAAATACATTCTGCTA+TGG 0.492784 8.3:-67183616 MS.gene84731:CDS
CTATTGGTGACTATGTCTAA+TGG 0.493151 8.3:-67185096 MS.gene84731:CDS
AGAGAAGTGCAATAGAGAAG+TGG 0.495497 8.3:-67185708 MS.gene84731:CDS
GCGAGCAGAGAGAGCAACAC+AGG 0.499770 8.3:-67185480 MS.gene84731:CDS
ATAGAGAAGTGGTTTGAAGA+AGG 0.503302 8.3:-67185697 MS.gene84731:CDS
GCTTTATTAACATTGATTGA+AGG 0.507058 8.3:-67183096 MS.gene84731:CDS
TCAGAATCTAGTAACAAAAC+CGG 0.507158 8.3:+67184407 None:intergenic
TGCCAATACTCTTGCTGACA+TGG 0.507732 8.3:+67182858 None:intergenic
TGTAAGAACAAGTGAAGAAG+AGG 0.509834 8.3:+67184644 None:intergenic
GCCGCATCAGGTCTTTCAGC+CGG 0.509865 8.3:-67186189 MS.gene84731:CDS
AGAGAGAGCAACACAGGGAA+TGG 0.509905 8.3:-67185474 MS.gene84731:CDS
GAAAGACCTGATGCGGCTAA+AGG 0.513497 8.3:+67186195 None:intergenic
AACTGAAATACTTTGCGAAA+CGG 0.517151 8.3:-67185837 MS.gene84731:CDS
ATCCATGTCAGCAAGAGTAT+TGG 0.518034 8.3:-67182860 MS.gene84731:CDS
TTTCCTTTCGTTCGGTGATG+AGG 0.520051 8.3:+67185858 None:intergenic
AGTTACTCCTTTGAAGATCT+CGG 0.524906 8.3:+67183493 None:intergenic
AAAAGATAATGAAGAAGCTA+AGG 0.530212 8.3:-67185353 MS.gene84731:intron
TTACTGCAACGTCTCTCCAC+AGG 0.531709 8.3:-67184970 MS.gene84731:intron
TGAAACAATCCATAGAAGAA+TGG 0.531982 8.3:-67185609 MS.gene84731:CDS
GAGGAAATACATTCTGCTAT+GGG 0.532753 8.3:-67183615 MS.gene84731:CDS
TCTCCAAGATTTCTTGTGAG+TGG 0.533221 8.3:+67183153 None:intergenic
TTGATGTGTGTATGCTATGA+TGG 0.534750 8.3:+67184501 None:intergenic
AACTTAGTCGAGAAAGCAGT+TGG 0.540179 8.3:-67183474 MS.gene84731:CDS
AAATGAGAAGTCGACGATGA+AGG 0.546935 8.3:-67185992 MS.gene84731:CDS
AATAAGGAGTCCTTATTTGA+CGG 0.547052 8.3:-67184680 MS.gene84731:CDS
GTTGATTCCTTTAGATACAT+CGG 0.548864 8.3:-67185653 MS.gene84731:CDS
GACTTAACTCAGAGGAGTAA+TGG 0.550076 8.3:-67182892 MS.gene84731:CDS
ACAATCCTCATATGATGAAG+AGG 0.555198 8.3:-67183634 MS.gene84731:CDS
TTGGACGACGTCAGGAGGAA+AGG 0.555434 8.3:-67185203 MS.gene84731:CDS
TTCTCTTGGACGACGTCAGG+AGG 0.555553 8.3:-67185208 MS.gene84731:CDS
TGAGAAATTGCAGTTGAGGT+AGG 0.556553 8.3:+67186531 None:intergenic
GAAGGTGAAAGACTAGAGAG+TGG 0.557912 8.3:-67183078 MS.gene84731:CDS
CAGTTCCAACACACTTGGAG+TGG 0.563324 8.3:-67183434 MS.gene84731:CDS
ACTCAATGGATTCTAGATGC+TGG 0.565189 8.3:-67183558 MS.gene84731:CDS
CAGCCTCATCACCGAACGAA+AGG 0.569243 8.3:-67185861 MS.gene84731:CDS
TGATCAGAATGTTATACTGA+TGG 0.569744 8.3:-67185016 MS.gene84731:CDS
TTAATCAGTGTGACACAACC+TGG 0.570231 8.3:-67184359 MS.gene84731:CDS
GAGCATGATTCCTTATATCA+CGG 0.577282 8.3:+67185398 None:intergenic
GATTTGAGAAATTGCAGTTG+AGG 0.578462 8.3:+67186527 None:intergenic
TGGAACTGTAAAACGACGTA+AGG 0.584828 8.3:+67183449 None:intergenic
AATGGCACGGTTGAATGCCT+CGG 0.584864 8.3:+67186368 None:intergenic
AAGGAGATAAGCAAGGCGCT+TGG 0.588368 8.3:-67186225 MS.gene84731:CDS
TGTGTATGCTATGATGGTAA+AGG 0.588633 8.3:+67184507 None:intergenic
TCTAAAGGAATCAACGTCAA+AGG 0.588862 8.3:+67185661 None:intergenic
AACTAGAGGCATTTGAGCTG+AGG 0.594631 8.3:+67182915 None:intergenic
TCCGGCTGAAAGACCTGATG+CGG 0.596788 8.3:+67186188 None:intergenic
TATCAAGACGAAGCTAAATG+AGG 0.597576 8.3:-67184276 MS.gene84731:intron
GAATGTTATACTGATGGCAA+AGG 0.598582 8.3:-67185010 MS.gene84731:CDS
GGTGAAAGACTAGAGAGTGG+TGG 0.599671 8.3:-67183075 MS.gene84731:CDS
GGACAATAAAATGACTGTAG+AGG 0.605850 8.3:+67185787 None:intergenic
CAAATAAGGACTCCTTATTG+TGG 0.607893 8.3:+67184684 None:intergenic
ACTTTACAGCCAATTTCGCT+CGG 0.608017 8.3:+67183237 None:intergenic
GAAAGCTGGATTTAAGGCTG+CGG 0.609553 8.3:-67186127 MS.gene84731:CDS
GTAGAGACCGAGATCTTCAA+AGG 0.609913 8.3:-67183500 MS.gene84731:CDS
AAATTGATCAGATAACTCAA+TGG 0.612214 8.3:-67183572 MS.gene84731:CDS
GTACATCATTAGCACTGCAT+AGG 0.612506 8.3:+67186484 None:intergenic
ATTTATAAATATGTTCAAGA+CGG 0.616007 8.3:-67183522 MS.gene84731:CDS
CTCTCAAGATATCCAAACGC+CGG 0.616232 8.3:-67184426 MS.gene84731:CDS
TTGGCTGTAAAGTTCATGGA+GGG 0.616439 8.3:-67183227 MS.gene84731:CDS
CGAGCAGAGAGAGCAACACA+GGG 0.621568 8.3:-67185479 MS.gene84731:CDS
CTCTGAGTTAAGTCAACTAG+AGG 0.627340 8.3:+67182901 None:intergenic
ACTTGAAATGATTCGACAAG+GGG 0.632906 8.3:-67184552 MS.gene84731:CDS
GACTTGAAATGATTCGACAA+GGG 0.634541 8.3:-67184553 MS.gene84731:CDS
GGACTTGAAATGATTCGACA+AGG 0.635847 8.3:-67184554 MS.gene84731:CDS
TACGACATTCCAGAGCAAGT+AGG 0.646446 8.3:+67186313 None:intergenic
AGACGAAGGTGACTGACACA+AGG 0.652496 8.3:+67184304 None:intergenic
GATTCATAATATCAATGGCA+CGG 0.654224 8.3:+67186355 None:intergenic
TATCACACATGATGTTATGG+TGG 0.655301 8.3:-67185764 MS.gene84731:CDS
GATTTGGCGCGAGAACACAT+TGG 0.659835 8.3:-67185141 MS.gene84731:CDS
CAGGGAATGGATGATACTTG+AGG 0.659956 8.3:-67185461 MS.gene84731:CDS
AAGGAGTCCTTATTTGACGG+TGG 0.660568 8.3:-67184677 MS.gene84731:CDS
ATCTGATTATGGAGTCTGTG+AGG 0.673837 8.3:-67183130 MS.gene84731:CDS
GTCTTGATATTTGAAGACGA+AGG 0.676219 8.3:+67184290 None:intergenic
TGGCTGTAAAGTTCATGGAG+GGG 0.683620 8.3:-67183226 MS.gene84731:CDS
TCACACGAAGGAGATAAGCA+AGG 0.684993 8.3:-67186232 MS.gene84731:CDS
CTCTAGTTGACTTAACTCAG+AGG 0.686980 8.3:-67182900 MS.gene84731:CDS
ATTGGCTGTAAAGTTCATGG+AGG 0.694336 8.3:-67183228 MS.gene84731:CDS
TGATCTTCAGGTGAAAACAG+TGG 0.704631 8.3:-67184618 MS.gene84731:CDS
TCGGTTAGAAAATCACACGA+AGG 0.714647 8.3:-67186244 MS.gene84731:CDS
GGAATGTCGTAAGAAGAGCA+AGG 0.723044 8.3:-67186301 MS.gene84731:CDS
TGGTAAAGGAGATCGAGCAA+CGG 0.729976 8.3:+67184521 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAAGAAATTTTATTATTAA+TGG + chr8.3:67186359-67186378 None:intergenic 10.0%
!! AAAATATCATTTCTGATATT+TGG + chr8.3:67184543-67184562 None:intergenic 15.0%
!! AATATTGGAAGAAATTAAAA+AGG - chr8.3:67183214-67183233 MS.gene84731:CDS 15.0%
!! ACTAGTATTAAAATAGAAAA+CGG + chr8.3:67184073-67184092 None:intergenic 15.0%
!! ATTTATAAATATGTTCAAGA+CGG - chr8.3:67185858-67185877 MS.gene84731:CDS 15.0%
!! TGAACATATTTATAAATGAT+TGG + chr8.3:67185855-67185874 None:intergenic 15.0%
!!! AATGACATTTTATAATTTTG+GGG + chr8.3:67185246-67185265 None:intergenic 15.0%
!!! GAATGACATTTTATAATTTT+GGG + chr8.3:67185247-67185266 None:intergenic 15.0%
!!! TCATATCTTTTATAAGAAAA+AGG + chr8.3:67184495-67184514 None:intergenic 15.0%
!!! TGAATGACATTTTATAATTT+TGG + chr8.3:67185248-67185267 None:intergenic 15.0%
!!! TTTATTTGTTAATGAATTGT+AGG - chr8.3:67183102-67183121 MS.gene84731:CDS 15.0%
!! AAAGAAAAAATACATCAGTT+TGG - chr8.3:67183830-67183849 MS.gene84731:intron 20.0%
!! AGACAATAAAAGTTAACAAA+TGG - chr8.3:67185404-67185423 MS.gene84731:CDS 20.0%
!! ATAAGAAAAAGGATTAGAAA+TGG + chr8.3:67184484-67184503 None:intergenic 20.0%
!! ATGAAAATAGTTTCAACATT+TGG + chr8.3:67185510-67185529 None:intergenic 20.0%
!! TAAAAAGGTTTGTGATAATA+TGG - chr8.3:67183229-67183248 MS.gene84731:CDS 20.0%
!! TCGAAAACTATTTATAACTA+CGG + chr8.3:67185145-67185164 None:intergenic 20.0%
!! TGAATTATCCAAATATGATT+TGG - chr8.3:67184223-67184242 MS.gene84731:intron 20.0%
!!! TGTGAATAAAGATAGTTTTA+AGG - chr8.3:67182920-67182939 MS.gene84731:CDS 20.0%
!!! TTTAATTTCTTCCAATATTC+CGG + chr8.3:67183213-67183232 None:intergenic 20.0%
! AAAAGATAATGAAGAAGCTA+AGG - chr8.3:67184027-67184046 MS.gene84731:intron 25.0%
! AAATTGATCAGATAACTCAA+TGG - chr8.3:67185808-67185827 MS.gene84731:CDS 25.0%
! AAGATATGAATGCAACATAA+AGG - chr8.3:67184506-67184525 MS.gene84731:CDS 25.0%
! AGATGATGTAAAGATGATTA+TGG - chr8.3:67184642-67184661 MS.gene84731:CDS 25.0%
! ATCTACATTAGTTGAATCAT+TGG - chr8.3:67184448-67184467 MS.gene84731:CDS 25.0%
! CAAGTTCTTATACAAATTCT+TGG - chr8.3:67183950-67183969 MS.gene84731:intron 25.0%
! TAAATGATTAAACAAACCTG+TGG + chr8.3:67184429-67184448 None:intergenic 25.0%
! TCTCAAATCATAGAAACTAT+TGG - chr8.3:67182867-67182886 MS.gene84731:CDS 25.0%
! TTCCTCAATTCTTTCAAAAT+CGG + chr8.3:67182975-67182994 None:intergenic 25.0%
! TTGCAAACATCAGTATAAAT+AGG - chr8.3:67185191-67185210 MS.gene84731:CDS 25.0%
!! GCTTTATTAACATTGATTGA+AGG - chr8.3:67186284-67186303 MS.gene84731:CDS 25.0%
!! TAAATTAGTTTCTTGTGAAG+TGG + chr8.3:67185363-67185382 None:intergenic 25.0%
!!! GATTGTTTCAAAGTTTTGTT+AGG + chr8.3:67183761-67183780 None:intergenic 25.0%
!!! TATAAAGTTGTTTTCATGCT+TGG - chr8.3:67185650-67185669 MS.gene84731:CDS 25.0%
AACTGAAATACTTTGCGAAA+CGG - chr8.3:67183543-67183562 MS.gene84731:CDS 30.0%
AAGTCCATTTCTGTTTAAAC+TGG + chr8.3:67184812-67184831 None:intergenic 30.0%
AATCTTGGAGAATCTGATTA+TGG - chr8.3:67186239-67186258 MS.gene84731:CDS 30.0%
AATGAGATAGAAACAGCTTA+TGG + chr8.3:67184608-67184627 None:intergenic 30.0%
ATCAACATTCTCTACTATGA+TGG + chr8.3:67185694-67185713 None:intergenic 30.0%
ATTGTAGGTCGATTGTAAAT+CGG - chr8.3:67183117-67183136 MS.gene84731:CDS 30.0%
CAAAAAATTGTAGCTGAAAC+TGG - chr8.3:67185969-67185988 MS.gene84731:CDS 30.0%
CATACACACATCAAGTTTAT+GGG - chr8.3:67184888-67184907 MS.gene84731:intron 30.0%
CTATCCAGTTTAAACAGAAA+TGG - chr8.3:67184805-67184824 MS.gene84731:intron 30.0%
GATTCATAATATCAATGGCA+CGG + chr8.3:67183028-67183047 None:intergenic 30.0%
GATTGAAACTTTACGATCTT+TGG - chr8.3:67185435-67185454 MS.gene84731:CDS 30.0%
GTTCTTACATAATGATCTTC+AGG - chr8.3:67184750-67184769 MS.gene84731:intron 30.0%
TAAAGATGCAACTGAAGTAT+TGG - chr8.3:67184325-67184344 MS.gene84731:CDS 30.0%
TATTTCAGTTTCCTTTCGTT+CGG + chr8.3:67183533-67183552 None:intergenic 30.0%
TATTTCCTCTTCATCATATG+AGG + chr8.3:67185754-67185773 None:intergenic 30.0%
TCAGAATCTAGTAACAAAAC+CGG + chr8.3:67184976-67184995 None:intergenic 30.0%
TCTATCTTTCCATTCTTCTA+TGG + chr8.3:67183783-67183802 None:intergenic 30.0%
TGAAACAATCCATAGAAGAA+TGG - chr8.3:67183771-67183790 MS.gene84731:CDS 30.0%
TGAGTTATCTGATCAATTTC+TGG + chr8.3:67185807-67185826 None:intergenic 30.0%
TGATCAGAATGTTATACTGA+TGG - chr8.3:67184364-67184383 MS.gene84731:CDS 30.0%
TTCGCGATTCATAATATCAA+TGG + chr8.3:67183033-67183052 None:intergenic 30.0%
TTGCCAATTCGAAAAAATGA+GGG + chr8.3:67185217-67185236 None:intergenic 30.0%
TTTGCCAATTCGAAAAAATG+AGG + chr8.3:67185218-67185237 None:intergenic 30.0%
! AACTAAACTTTCTTTTCTGC+AGG - chr8.3:67184106-67184125 MS.gene84731:intron 30.0%
! AATAAGGAGTCCTTATTTGA+CGG - chr8.3:67184700-67184719 MS.gene84731:CDS 30.0%
! CAAGTCATCATTTTGTCTTT+TGG - chr8.3:67186187-67186206 MS.gene84731:CDS 30.0%
! GTTGATTCCTTTAGATACAT+CGG - chr8.3:67183727-67183746 MS.gene84731:CDS 30.0%
! GTTGTAGATTTTCTTAGTTC+TGG - chr8.3:67182825-67182844 MS.gene84731:CDS 30.0%
! TAAGGAACTTAGCTCTTATT+TGG - chr8.3:67182938-67182957 MS.gene84731:CDS 30.0%
! TAGATTCTTAAGAGCTTTTG+TGG + chr8.3:67184788-67184807 None:intergenic 30.0%
! TATCTTTTGCTAGAATGCAA+AGG + chr8.3:67184015-67184034 None:intergenic 30.0%
!! ATTTTAGAGAAAATCGAGTC+AGG - chr8.3:67183290-67183309 MS.gene84731:CDS 30.0%
!! TTTTAGAGAAAATCGAGTCA+GGG - chr8.3:67183291-67183310 MS.gene84731:CDS 30.0%
AAGTTGTCTAAAAACTCTCG+AGG - chr8.3:67183871-67183890 MS.gene84731:intron 35.0%
ACAATCCTCATATGATGAAG+AGG - chr8.3:67185746-67185765 MS.gene84731:CDS 35.0%
ACCATAACATCATGTGTGAT+AGG + chr8.3:67183617-67183636 None:intergenic 35.0%
ACTTGAAATGATTCGACAAG+GGG - chr8.3:67184828-67184847 MS.gene84731:intron 35.0%
ACTTTATAGCACTTGCTCTT+AGG + chr8.3:67185638-67185657 None:intergenic 35.0%
AGAGGAAATACATTCTGCTA+TGG - chr8.3:67185764-67185783 MS.gene84731:CDS 35.0%
ATAGAAACAGCTTATGGTTC+TGG + chr8.3:67184602-67184621 None:intergenic 35.0%
ATAGAGAAGTGGTTTGAAGA+AGG - chr8.3:67183683-67183702 MS.gene84731:CDS 35.0%
ATCTTTAGGTACTCGAATCT+TGG - chr8.3:67183568-67183587 MS.gene84731:CDS 35.0%
ATGTGAGATTAATGCGCAAT+TGG + chr8.3:67185334-67185353 None:intergenic 35.0%
CAAATAAGGACTCCTTATTG+TGG + chr8.3:67184699-67184718 None:intergenic 35.0%
CTCAAAACCGATGTATCTAA+AGG + chr8.3:67183737-67183756 None:intergenic 35.0%
CTTCATCATATGAGGATTGT+AGG + chr8.3:67185746-67185765 None:intergenic 35.0%
GAAATTGGCTGTAAAGTTCA+TGG - chr8.3:67186149-67186168 MS.gene84731:CDS 35.0%
GAACACATTGGAAAAGTTCA+AGG - chr8.3:67184251-67184270 MS.gene84731:intron 35.0%
GAATGTTATACTGATGGCAA+AGG - chr8.3:67184370-67184389 MS.gene84731:CDS 35.0%
GACTTGAAATGATTCGACAA+GGG - chr8.3:67184827-67184846 MS.gene84731:intron 35.0%
GAGCATGATTCCTTATATCA+CGG + chr8.3:67183985-67184004 None:intergenic 35.0%
GAGGAAATACATTCTGCTAT+GGG - chr8.3:67185765-67185784 MS.gene84731:CDS 35.0%
GATTTGAGAAATTGCAGTTG+AGG + chr8.3:67182856-67182875 None:intergenic 35.0%
GCATACACACATCAAGTTTA+TGG - chr8.3:67184887-67184906 MS.gene84731:intron 35.0%
GGAATTAACTGACCACAATA+AGG - chr8.3:67184684-67184703 MS.gene84731:CDS 35.0%
GGACAATAAAATGACTGTAG+AGG + chr8.3:67183596-67183615 None:intergenic 35.0%
GGATCAAATCATTGCTTTGT+TGG - chr8.3:67183484-67183503 MS.gene84731:CDS 35.0%
GTCTTGATATTTGAAGACGA+AGG + chr8.3:67185093-67185112 None:intergenic 35.0%
GTGGTCAGTTAATTCCATTT+CGG + chr8.3:67184680-67184699 None:intergenic 35.0%
TAACGACAGAAGTTAACAGA+TGG + chr8.3:67185278-67185297 None:intergenic 35.0%
TATCAAGACGAAGCTAAATG+AGG - chr8.3:67185104-67185123 MS.gene84731:CDS 35.0%
TATCACACATGATGTTATGG+TGG - chr8.3:67183616-67183635 MS.gene84731:CDS 35.0%
TATGGAGAAAGCTGGATTTA+AGG - chr8.3:67183247-67183266 MS.gene84731:CDS 35.0%
TCAAGGTTGCATACTTCTAT+TGG - chr8.3:67184268-67184287 MS.gene84731:intron 35.0%
TCCTATCACACATGATGTTA+TGG - chr8.3:67183613-67183632 MS.gene84731:CDS 35.0%
TCTAAAGGAATCAACGTCAA+AGG + chr8.3:67183722-67183741 None:intergenic 35.0%
TGATCTGATGAAGTTTCAAC+AGG + chr8.3:67183644-67183663 None:intergenic 35.0%
TGCTGCAAGTTGCATAATTA+TGG + chr8.3:67184926-67184945 None:intergenic 35.0%
TGTAAGAACAAGTGAAGAAG+AGG + chr8.3:67184739-67184758 None:intergenic 35.0%
TGTGATAATATGGAGAAAGC+TGG - chr8.3:67183239-67183258 MS.gene84731:CDS 35.0%
TGTGTATGCTATGATGGTAA+AGG + chr8.3:67184876-67184895 None:intergenic 35.0%
TTAGTGCAGTTGCTTGAAAT+TGG - chr8.3:67186005-67186024 MS.gene84731:CDS 35.0%
TTCCATTTCGGCTAATGTAT+TGG + chr8.3:67184668-67184687 None:intergenic 35.0%
TTCTATCTCATTATCTGTGC+AGG - chr8.3:67184616-67184635 MS.gene84731:CDS 35.0%
TTGATGTGTGTATGCTATGA+TGG + chr8.3:67184882-67184901 None:intergenic 35.0%
! AGTTACTCCTTTGAAGATCT+CGG + chr8.3:67185890-67185909 None:intergenic 35.0%
! CATAGTAGTCCTTTTTGCTT+CGG + chr8.3:67186110-67186129 None:intergenic 35.0%
! CTATTGGTGACTATGTCTAA+TGG - chr8.3:67184284-67184303 MS.gene84731:CDS 35.0%
! GTTGTGTCACACTGATTAAA+GGG + chr8.3:67185020-67185039 None:intergenic 35.0%
! TCACACTGATTAAAGGGAAA+AGG + chr8.3:67185014-67185033 None:intergenic 35.0%
! TCTGATCAATTTCTGGAAGA+TGG + chr8.3:67185800-67185819 None:intergenic 35.0%
! TGCTCGTGTTGATAAGAAAA+AGG - chr8.3:67183445-67183464 MS.gene84731:CDS 35.0%
! TTTTACAGTTCCAACACACT+TGG - chr8.3:67185941-67185960 MS.gene84731:CDS 35.0%
!! AGTCTCTACATGCTTTAGAA+AGG - chr8.3:67186405-67186424 MS.gene84731:CDS 35.0%
!! ATAATGTTTTGTTGCACACC+AGG + chr8.3:67185042-67185061 None:intergenic 35.0%
!! CGCTGAATTTTCTAAAAGCT+CGG - chr8.3:67186064-67186083 MS.gene84731:CDS 35.0%
!! TTCTTAGTTCTACCTCTCTT+GGG - chr8.3:67186372-67186391 MS.gene84731:CDS 35.0%
!! TTTCTTAGTTCTACCTCTCT+TGG - chr8.3:67186371-67186390 MS.gene84731:CDS 35.0%
AAAGGCTGAAGCTATGCAAA+TGG - chr8.3:67183463-67183482 MS.gene84731:CDS 40.0%
AAATGAGAAGTCGACGATGA+AGG - chr8.3:67183388-67183407 MS.gene84731:CDS 40.0%
AACTTAGTCGAGAAAGCAGT+TGG - chr8.3:67185906-67185925 MS.gene84731:CDS 40.0%
AAGTACACCACCGTCAAATA+AGG + chr8.3:67184713-67184732 None:intergenic 40.0%
AATCTCATTGCTAACGCTGT+TGG - chr8.3:67183359-67183378 MS.gene84731:CDS 40.0%
ACTTTACAGCCAATTTCGCT+CGG + chr8.3:67186146-67186165 None:intergenic 40.0%
AGAAAATCGAGTCAGGGATA+AGG - chr8.3:67183297-67183316 MS.gene84731:CDS 40.0%
ATCTGATTATGGAGTCTGTG+AGG - chr8.3:67186250-67186269 MS.gene84731:CDS 40.0%
ATTGGCTGTAAAGTTCATGG+AGG - chr8.3:67186152-67186171 MS.gene84731:CDS 40.0%
CAGCTATACCAGAATTGGTT+AGG - chr8.3:67185589-67185608 MS.gene84731:CDS 40.0%
CTATGATGTGGCACGTTAAT+TGG - chr8.3:67185302-67185321 MS.gene84731:intron 40.0%
CTCTGAGTTAAGTCAACTAG+AGG + chr8.3:67186482-67186501 None:intergenic 40.0%
CTGTTAACTTCTGTCGTTAG+AGG - chr8.3:67185279-67185298 MS.gene84731:intron 40.0%
GAAAATCGAGTCAGGGATAA+GGG - chr8.3:67183298-67183317 MS.gene84731:CDS 40.0%
GAATCGATAGTCCATTCTCT+TGG - chr8.3:67184158-67184177 MS.gene84731:intron 40.0%
GACTTAACTCAGAGGAGTAA+TGG - chr8.3:67186488-67186507 MS.gene84731:CDS 40.0%
GATTAATGCGCAATTGGACA+TGG + chr8.3:67185328-67185347 None:intergenic 40.0%
GCAGAGTTCAAACAGTTGTA+TGG - chr8.3:67186440-67186459 MS.gene84731:CDS 40.0%
GGACTTGAAATGATTCGACA+AGG - chr8.3:67184826-67184845 MS.gene84731:intron 40.0%
GTACATCATTAGCACTGCAT+AGG + chr8.3:67182899-67182918 None:intergenic 40.0%
GTTCGAGAAACCGTGATATA+AGG - chr8.3:67183972-67183991 MS.gene84731:intron 40.0%
TCACACAACCTAACCAATTC+TGG + chr8.3:67185600-67185619 None:intergenic 40.0%
TCGGTTAGAAAATCACACGA+AGG - chr8.3:67183136-67183155 MS.gene84731:CDS 40.0%
TCTCCAAGATTTCTTGTGAG+TGG + chr8.3:67186230-67186249 None:intergenic 40.0%
TGAAAAAGTGAGTGATTCCG+AGG - chr8.3:67182995-67183014 MS.gene84731:CDS 40.0%
TGAGAAATTGCAGTTGAGGT+AGG + chr8.3:67182852-67182871 None:intergenic 40.0%
TGATCTTCAGGTGAAAACAG+TGG - chr8.3:67184762-67184781 MS.gene84731:intron 40.0%
TGGAACTGTAAAACGACGTA+AGG + chr8.3:67185934-67185953 None:intergenic 40.0%
TTAATCAGTGTGACACAACC+TGG - chr8.3:67185021-67185040 MS.gene84731:CDS 40.0%
TTCTCGCGCCAAATCATATT+TGG + chr8.3:67184234-67184253 None:intergenic 40.0%
TTGGCTGTAAAGTTCATGGA+GGG - chr8.3:67186153-67186172 MS.gene84731:CDS 40.0%
TTTGCGAAACGGCAATCTTT+AGG - chr8.3:67183554-67183573 MS.gene84731:CDS 40.0%
! ACTCAATGGATTCTAGATGC+TGG - chr8.3:67185822-67185841 MS.gene84731:CDS 40.0%
! AGAGAAGTGCAATAGAGAAG+TGG - chr8.3:67183672-67183691 MS.gene84731:CDS 40.0%
! AGTCCTTTTTGCTTCGGAAT+CGG + chr8.3:67186104-67186123 None:intergenic 40.0%
! ATCCATGTCAGCAAGAGTAT+TGG - chr8.3:67186520-67186539 MS.gene84731:CDS 40.0%
! CTCTAGTTGACTTAACTCAG+AGG - chr8.3:67186480-67186499 MS.gene84731:CDS 40.0%
! CTGAGCACTGTTCATACATA+GGG + chr8.3:67185572-67185591 None:intergenic 40.0%
! GGTTGTGTCACACTGATTAA+AGG + chr8.3:67185021-67185040 None:intergenic 40.0%
! TGAGCACTGTTCATACATAG+GGG + chr8.3:67185571-67185590 None:intergenic 40.0%
!!! AGGCCCTCATTTTTTCGAAT+TGG - chr8.3:67185211-67185230 MS.gene84731:CDS 40.0%
!!! CGCCGATTTTGAAAGAATTG+AGG - chr8.3:67182970-67182989 MS.gene84731:CDS 40.0%
AGGTCTTTCAGCCGGAATAT+TGG - chr8.3:67183199-67183218 MS.gene84731:CDS 45.0%
CAGGGAATGGATGATACTTG+AGG - chr8.3:67183919-67183938 MS.gene84731:intron 45.0%
CTAGTAACAAAACCGGCGTT+TGG + chr8.3:67184969-67184988 None:intergenic 45.0%
CTCTCAAGATATCCAAACGC+CGG - chr8.3:67184954-67184973 MS.gene84731:intron 45.0%
GAAGGTGAAAGACTAGAGAG+TGG - chr8.3:67186302-67186321 MS.gene84731:CDS 45.0%
GGAATGTCGTAAGAAGAGCA+AGG - chr8.3:67183079-67183098 MS.gene84731:CDS 45.0%
GGACCACTCACAAGAAATCT+TGG - chr8.3:67186224-67186243 MS.gene84731:CDS 45.0%
GGCCAATACATTAGCCGAAA+TGG - chr8.3:67184663-67184682 MS.gene84731:CDS 45.0%
GTAGAGACCGAGATCTTCAA+AGG - chr8.3:67185880-67185899 MS.gene84731:CDS 45.0%
GTGCTCAGCTATACCAGAAT+TGG - chr8.3:67185584-67185603 MS.gene84731:CDS 45.0%
TACGACATTCCAGAGCAAGT+AGG + chr8.3:67183070-67183089 None:intergenic 45.0%
TCACACGAAGGAGATAAGCA+AGG - chr8.3:67183148-67183167 MS.gene84731:CDS 45.0%
TGACTGACACAAGGCGTAAA+AGG + chr8.3:67185070-67185089 None:intergenic 45.0%
TGCCAATACTCTTGCTGACA+TGG + chr8.3:67186525-67186544 None:intergenic 45.0%
TGGCTGTAAAGTTCATGGAG+GGG - chr8.3:67186154-67186173 MS.gene84731:CDS 45.0%
TGGTAAAGGAGATCGAGCAA+CGG + chr8.3:67184862-67184881 None:intergenic 45.0%
TGTCGTTAGAGGCTATGATG+TGG - chr8.3:67185290-67185309 MS.gene84731:intron 45.0%
! AAGGAGTCCTTATTTGACGG+TGG - chr8.3:67184703-67184722 MS.gene84731:CDS 45.0%
! AGACCGATTCCGAAGCAAAA+AGG - chr8.3:67186098-67186117 MS.gene84731:CDS 45.0%
! CTTTTCTTGCAACCCAAGAG+AGG + chr8.3:67186387-67186406 None:intergenic 45.0%
! CTTTTGGCAGCTGAAGCAAT+TGG - chr8.3:67186203-67186222 MS.gene84731:CDS 45.0%
! GAAAGCTGGATTTAAGGCTG+CGG - chr8.3:67183253-67183272 MS.gene84731:CDS 45.0%
! GAGCACTGTTCATACATAGG+GGG + chr8.3:67185570-67185589 None:intergenic 45.0%
! GCTGAGCACTGTTCATACAT+AGG + chr8.3:67185573-67185592 None:intergenic 45.0%
! TTTCCTTTCGTTCGGTGATG+AGG + chr8.3:67183525-67183544 None:intergenic 45.0%
! TTTTGCCACTCCAAGTGTGT+TGG + chr8.3:67185954-67185973 None:intergenic 45.0%
!! AACTAGAGGCATTTGAGCTG+AGG + chr8.3:67186468-67186487 None:intergenic 45.0%
AATGGCACGGTTGAATGCCT+CGG + chr8.3:67183015-67183034 None:intergenic 50.0%
AGACGAAGGTGACTGACACA+AGG + chr8.3:67185079-67185098 None:intergenic 50.0%
AGAGAGAGCAACACAGGGAA+TGG - chr8.3:67183906-67183925 MS.gene84731:intron 50.0%
AGCTGCAAACCTACTTGCTC+TGG - chr8.3:67183058-67183077 MS.gene84731:CDS 50.0%
CAGTTCCAACACACTTGGAG+TGG - chr8.3:67185946-67185965 MS.gene84731:CDS 50.0%
CTTCTTCCTTTAGCCGCATC+AGG - chr8.3:67183179-67183198 MS.gene84731:CDS 50.0%
GAAAGACCTGATGCGGCTAA+AGG + chr8.3:67183188-67183207 None:intergenic 50.0%
GATTTGGCGCGAGAACACAT+TGG - chr8.3:67184239-67184258 MS.gene84731:intron 50.0%
GGTGAAAGACTAGAGAGTGG+TGG - chr8.3:67186305-67186324 MS.gene84731:CDS 50.0%
TGGAGTCTGTGAGGCTTCTT+TGG - chr8.3:67186259-67186278 MS.gene84731:CDS 50.0%
TTACTGCAACGTCTCTCCAC+AGG - chr8.3:67184410-67184429 MS.gene84731:CDS 50.0%
TTCTCAGCTCCGAGCGAAAT+TGG - chr8.3:67186134-67186153 MS.gene84731:CDS 50.0%
! AAGGAGATAAGCAAGGCGCT+TGG - chr8.3:67183155-67183174 MS.gene84731:CDS 50.0%
! TGGATTTAAGGCTGCGGTAG+CGG - chr8.3:67183259-67183278 MS.gene84731:CDS 50.0%
!! CGCTGCACGTTGTTTTGTCA+AGG + chr8.3:67186036-67186055 None:intergenic 50.0%
CAGCCTCATCACCGAACGAA+AGG - chr8.3:67183519-67183538 MS.gene84731:CDS 55.0%
CCATTCTCTTGGACGACGTC+AGG - chr8.3:67184169-67184188 MS.gene84731:intron 55.0%
CCTGACGTCGTCCAAGAGAA+TGG + chr8.3:67184172-67184191 None:intergenic 55.0%
CGAGCAGAGAGAGCAACACA+GGG - chr8.3:67183901-67183920 MS.gene84731:intron 55.0%
TCCGGCTGAAAGACCTGATG+CGG + chr8.3:67183195-67183214 None:intergenic 55.0%
TTCTCTTGGACGACGTCAGG+AGG - chr8.3:67184172-67184191 MS.gene84731:intron 55.0%
TTGGACGACGTCAGGAGGAA+AGG - chr8.3:67184177-67184196 MS.gene84731:intron 55.0%
GCCGCATCAGGTCTTTCAGC+CGG - chr8.3:67183191-67183210 MS.gene84731:CDS 60.0%
GCGAGCAGAGAGAGCAACAC+AGG - chr8.3:67183900-67183919 MS.gene84731:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 67182819 67186583 67182819 ID=MS.gene84731
chr8.3 mRNA 67182819 67186583 67182819 ID=MS.gene84731.t1;Parent=MS.gene84731
chr8.3 exon 67185354 67186583 67185354 ID=MS.gene84731.t1.exon1;Parent=MS.gene84731.t1
chr8.3 CDS 67185354 67186583 67185354 ID=cds.MS.gene84731.t1;Parent=MS.gene84731.t1
chr8.3 exon 67184971 67185274 67184971 ID=MS.gene84731.t1.exon2;Parent=MS.gene84731.t1
chr8.3 CDS 67184971 67185274 67184971 ID=cds.MS.gene84731.t1;Parent=MS.gene84731.t1
chr8.3 exon 67184277 67184764 67184277 ID=MS.gene84731.t1.exon3;Parent=MS.gene84731.t1
chr8.3 CDS 67184277 67184764 67184277 ID=cds.MS.gene84731.t1;Parent=MS.gene84731.t1
chr8.3 exon 67182819 67183817 67182819 ID=MS.gene84731.t1.exon4;Parent=MS.gene84731.t1
chr8.3 CDS 67182819 67183817 67182819 ID=cds.MS.gene84731.t1;Parent=MS.gene84731.t1
Gene Sequence

>MS.gene84731

ATGATGGTTGTAGATTTTCTTAGTTCTGGTCCTACCTCAACTGCAATTTCTCAAATCATAGAAACTATTGGTGAGTTCCTATGCAGTGCTAATGATGTACTTGTGAATAAAGATAGTTTTAAGGAACTTAGCTCTTATTTGGAAAGAATTACGCCGATTTTGAAAGAATTGAGGAATGAAAAAGTGAGTGATTCCGAGGCATTCAACCGTGCCATTGATATTATGAATCGCGAAACGAAAGCTGCAAACCTACTTGCTCTGGAATGTCGTAAGAAGAGCAAGGTTTATTTGTTAATGAATTGTAGGTCGATTGTAAATCGGTTAGAAAATCACACGAAGGAGATAAGCAAGGCGCTTGGTCTTCTTCCTTTAGCCGCATCAGGTCTTTCAGCCGGAATATTGGAAGAAATTAAAAAGGTTTGTGATAATATGGAGAAAGCTGGATTTAAGGCTGCGGTAGCGGAAGAAGAGATTTTAGAGAAAATCGAGTCAGGGATAAGGGAAAACAGTTTTGATCGTAAGCATGCAAATAATTTAATAAATCTCATTGCTAACGCTGTTGGAATTACAAATGAGAAGTCGACGATGAAGGCTGAACTTGAAGAGTTTAAAAAAGAAATCGAAAATGCTCGTGTTGATAAGAAAAAGGCTGAAGCTATGCAAATGGATCAAATCATTGCTTTGTTGGAAAGAGCTGATGCAGCCTCATCACCGAACGAAAGGAAACTGAAATACTTTGCGAAACGGCAATCTTTAGGTACTCGAATCTTGGAGCCTCTACAGTCATTTTATTGTCCTATCACACATGATGTTATGGTGGAACCTGTTGAAACTTCATCAGATCAAACATTTGAGAGAAGTGCAATAGAGAAGTGGTTTGAAGAAGGAAACAAGTTGTGTCCTTTGACGTTGATTCCTTTAGATACATCGGTTTTGAGACCTAACAAAACTTTGAAACAATCCATAGAAGAATGGAAAGATAGAAATACAATGATTACAATTGCTACTTTGAAAGAAAAAATACATCAGTTTGGAGATGATGATGAAGTGATAAGTTGTCTAAAAACTCTCGAGGATTTGTGCGAGCAGAGAGAGCAACACAGGGAATGGATGATACTTGAGGACTACATACAAGTTCTTATACAAATTCTTGGTTCGAGAAACCGTGATATAAGGAATCATGCTCTTGTCATCCTTTGCATTCTAGCAAAAGATAATGAAGAAGCTAAGGTACTTAAATGAAAATACTATCCGTTTTCTATTTTAATACTAGTTTACACATTTGAGAACTAAACTTTCTTTTCTGCAGGAAAGAATTGTAACAGTTGATAATGCGATTGAATCGATAGTCCATTCTCTTGGACGACGTCAGGAGGAAAGGAAGTTAGCAGTTGCATTACTGCTTGAATTATCCAAATATGATTTGGCGCGAGAACACATTGGAAAAGTTCAAGGTTGCATACTTCTATTGGTGACTATGTCTAATGGTGATGACAATCAAGCTGCTAAAGATGCAACTGAAGTATTGGATAATCTTTCATACTCTGATCAGAATGTTATACTGATGGCAAAGGCAAACTATTTCAAGCATTTACTGCAACGTCTCTCCACAGGTTTGTTTAATCATTTATCTACATTAGTTGAATCATTGGTATTAGAGTTCCATTTCTAATCCTTTTTCTTATAAAAGATATGAATGCAACATAAAGGAGCATATGCATCCAAATATCAGAAATGATATTTTGACACAGAAATTTGACAACAATTTAGAAGATTAATGCCAGAACCATAAGCTGTTTCTATCTCATTATCTGTGCAGGAGCAGATGATGTAAAGATGATTATGGCCAATACATTAGCCGAAATGGAATTAACTGACCACAATAAGGAGTCCTTATTTGACGGTGGTGTACTTGCTCCTCTTCTTCACTTGTTCTTACATAATGATCTTCAGGTGAAAACAGTGGCCACAAAAGCTCTTAAGAATCTATCCAGTTTAAACAGAAATGGACTTGAAATGATTCGACAAGGGGCAGTGCGACCGTTGCTCGATCTCCTTTACCATCATAGCATACACACATCAAGTTTATGGGAAGATGTAGCAGCCATAATTATGCAACTTGCAGCATCAACTATCTCTCAAGATATCCAAACGCCGGTTTTGTTACTAGATTCTGATGATGATGTTTTTAACCTTTTCCCTTTAATCAGTGTGACACAACCTGGTGTGCAACAAAACATTATTCAGACCTTTTACGCCTTGTGTCAGTCACCTTCGTCTTCAAATATCAAGACGAAGCTAAATGAGGTATGAATCTTACTGACCGTAGTTATAAATAGTTTTCGAGTGAATATGCAAATTGATCATTGAAATTGCAAACATCAGTATAAATAGGCCCTCATTTTTTCGAATTGGCAAATACATCCCCAAAATTATAAAATGTCATTCAAAATAGTCCATCTGTTAACTTCTGTCGTTAGAGGCTATGATGTGGCACGTTAATTGGCCATGTCCAATTGCGCATTAATCTCACATCAATGTCCACTTCACAAGAAACTAATTTATTTATAGTCACATCATAGTCTAGACAATAAAAGTTAACAAATGGACTATTTTGATTGAAACTTTACGATCTTTGGTTTTTAAAGTTCACTCTCTACTATTCTTTCATTCATGTACTTGTCTTTTCCAAATGTTGAAACTATTTTCATGTTTTTGTTTATGTTAATATACTATAACTAAATGAGTCCCCCTATGTATGAACAGTGCTCAGCTATACCAGAATTGGTTAGGTTGTGTGAGAATGAGAATCTAAACCTAAGAGCAAGTGCTATAAAGTTGTTTTCATGCTTGGTAGAGAGTTGTGATGAATCCATCATAGTAGAGAATGTTGATCAGAAATGCATCAATACTTTACTTCAGATCCTACAATCCTCATATGATGAAGAGGAAATACATTCTGCTATGGGAATAATATGCCATCTTCCAGAAATTGATCAGATAACTCAATGGATTCTAGATGCTGGCGTACTTCCAATCATTTATAAATATGTTCAAGACGGTAGAGACCGAGATCTTCAAAGGAGTAACTTAGTCGAGAAAGCAGTTGGTGCCTTACGTCGTTTTACAGTTCCAACACACTTGGAGTGGCAAAAAATTGTAGCTGAAACTGGTATTATAACTGTTTTAGTGCAGTTGCTTGAAATTGGATCAACCTTGACAAAACAACGTGCAGCGTTATGTCTCGCTGAATTTTCTAAAAGCTCGGTTAGTCTCAGCAGACCGATTCCGAAGCAAAAAGGACTACTATGTTGTTTCTCAGCTCCGAGCGAAATTGGCTGTAAAGTTCATGGAGGGGTATGCACTGTCAAGTCATCATTTTGTCTTTTGGCAGCTGAAGCAATTGGACCACTCACAAGAAATCTTGGAGAATCTGATTATGGAGTCTGTGAGGCTTCTTTGGATGCTTTATTAACATTGATTGAAGGTGAAAGACTAGAGAGTGGTGGTAAAGTGCTTGCTAAAGCAAATGCAATACCATTAATAATAAAATTTCTTAGTTCTACCTCTCTTGGGTTGCAAGAAAAGTCTCTACATGCTTTAGAAAGGATTTTTCAGCTAGCAGAGTTCAAACAGTTGTATGGAGCCTCAGCTCAAATGCCTCTAGTTGACTTAACTCAGAGGAGTAATGGTAGAGTTAGATCCATGTCAGCAAGAGTATTGGCACATTTGAATGTTCTTCATGATCAGTCTTCATACTTTTAA
Protein sequence

>MS.gene84731.t1

MMVVDFLSSGPTSTAISQIIETIGEFLCSANDVLVNKDSFKELSSYLERITPILKELRNEKVSDSEAFNRAIDIMNRETKAANLLALECRKKSKVYLLMNCRSIVNRLENHTKEISKALGLLPLAASGLSAGILEEIKKVCDNMEKAGFKAAVAEEEILEKIESGIRENSFDRKHANNLINLIANAVGITNEKSTMKAELEEFKKEIENARVDKKKAEAMQMDQIIALLERADAASSPNERKLKYFAKRQSLGTRILEPLQSFYCPITHDVMVEPVETSSDQTFERSAIEKWFEEGNKLCPLTLIPLDTSVLRPNKTLKQSIEEWKDRNTMITIATLKEKIHQFGDDDEVISCLKTLEDLCEQREQHREWMILEDYIQVLIQILGSRNRDIRNHALVILCILAKDNEEAKERIVTVDNAIESIVHSLGRRQEERKLAVALLLELSKYDLAREHIGKVQGCILLLVTMSNGDDNQAAKDATEVLDNLSYSDQNVILMAKANYFKHLLQRLSTGADDVKMIMANTLAEMELTDHNKESLFDGGVLAPLLHLFLHNDLQVKTVATKALKNLSSLNRNGLEMIRQGAVRPLLDLLYHHSIHTSSLWEDVAAIIMQLAASTISQDIQTPVLLLDSDDDVFNLFPLISVTQPGVQQNIIQTFYALCQSPSSSNIKTKLNECSAIPELVRLCENENLNLRASAIKLFSCLVESCDESIIVENVDQKCINTLLQILQSSYDEEEIHSAMGIICHLPEIDQITQWILDAGVLPIIYKYVQDGRDRDLQRSNLVEKAVGALRRFTVPTHLEWQKIVAETGIITVLVQLLEIGSTLTKQRAALCLAEFSKSSVSLSRPIPKQKGLLCCFSAPSEIGCKVHGGVCTVKSSFCLLAAEAIGPLTRNLGESDYGVCEASLDALLTLIEGERLESGGKVLAKANAIPLIIKFLSSTSLGLQEKSLHALERIFQLAEFKQLYGASAQMPLVDLTQRSNGRVRSMSARVLAHLNVLHDQSSYF