Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84842.t1 | XP_004510773.1 | 100 | 59 | 0 | 0 | 1 | 59 | 1043 | 1101 | 4.20E-27 | 129.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84842.t1 | Q6EVK6 | 84.7 | 59 | 9 | 0 | 1 | 59 | 1016 | 1074 | 6.3e-24 | 110.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84842.t1 | G7LBQ1 | 100.0 | 59 | 0 | 0 | 1 | 59 | 1022 | 1080 | 3.0e-27 | 129.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene84842.t1 | TR | SNF2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051800 | MS.gene84842 | 0.806706 | 7.22E-50 | -1.69E-46 |
| MS.gene052609 | MS.gene84842 | 0.808254 | 3.39E-50 | -1.69E-46 |
| MS.gene056507 | MS.gene84842 | 0.817365 | 3.43E-52 | -1.69E-46 |
| MS.gene058289 | MS.gene84842 | 0.807119 | 5.91E-50 | -1.69E-46 |
| MS.gene058647 | MS.gene84842 | 0.816573 | 5.16E-52 | -1.69E-46 |
| MS.gene058919 | MS.gene84842 | 0.803335 | 3.66E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene84842 | 0.825664 | 4.14E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84842.t1 | MTR_8g030550 | 100.000 | 59 | 0 | 0 | 1 | 59 | 1022 | 1080 | 4.88e-39 | 135 |
| MS.gene84842.t1 | MTR_8g030550 | 100.000 | 59 | 0 | 0 | 1 | 59 | 1022 | 1080 | 4.88e-39 | 135 |
| MS.gene84842.t1 | MTR_8g030550 | 100.000 | 59 | 0 | 0 | 1 | 59 | 1022 | 1080 | 4.88e-39 | 135 |
| MS.gene84842.t1 | MTR_7g078090 | 86.441 | 59 | 8 | 0 | 1 | 59 | 1029 | 1087 | 3.65e-33 | 119 |
| MS.gene84842.t1 | MTR_4g118720 | 45.763 | 59 | 32 | 0 | 1 | 59 | 1040 | 1098 | 9.21e-14 | 63.5 |
| MS.gene84842.t1 | MTR_5g005840 | 45.455 | 55 | 30 | 0 | 2 | 56 | 399 | 453 | 6.09e-13 | 61.2 |
| MS.gene84842.t1 | MTR_1g105050 | 47.273 | 55 | 29 | 0 | 2 | 56 | 412 | 466 | 7.19e-13 | 61.2 |
| MS.gene84842.t1 | MTR_1g053955 | 46.154 | 52 | 28 | 0 | 2 | 53 | 557 | 608 | 7.78e-12 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84842.t1 | AT2G46020 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1016 | 1074 | 4.88e-32 | 115 |
| MS.gene84842.t1 | AT2G46020 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1016 | 1074 | 4.88e-32 | 115 |
| MS.gene84842.t1 | AT2G46020 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1016 | 1074 | 4.88e-32 | 115 |
| MS.gene84842.t1 | AT2G46020 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1016 | 1074 | 4.88e-32 | 115 |
| MS.gene84842.t1 | AT2G46020 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1016 | 1074 | 4.88e-32 | 115 |
| MS.gene84842.t1 | AT2G46020 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1016 | 1074 | 4.88e-32 | 115 |
| MS.gene84842.t1 | AT5G19310 | 50.909 | 55 | 27 | 0 | 2 | 56 | 158 | 212 | 5.05e-15 | 67.4 |
| MS.gene84842.t1 | AT5G19310 | 50.909 | 55 | 27 | 0 | 2 | 56 | 422 | 476 | 5.07e-15 | 67.4 |
| MS.gene84842.t1 | AT2G28290 | 47.458 | 59 | 31 | 0 | 1 | 59 | 789 | 847 | 3.05e-14 | 65.1 |
| MS.gene84842.t1 | AT2G28290 | 47.458 | 59 | 31 | 0 | 1 | 59 | 789 | 847 | 3.05e-14 | 65.1 |
| MS.gene84842.t1 | AT2G28290 | 47.458 | 59 | 31 | 0 | 1 | 59 | 789 | 847 | 3.05e-14 | 65.1 |
| MS.gene84842.t1 | AT2G28290 | 47.458 | 59 | 31 | 0 | 1 | 59 | 790 | 848 | 3.05e-14 | 65.1 |
| MS.gene84842.t1 | AT2G28290 | 47.458 | 59 | 31 | 0 | 1 | 59 | 789 | 847 | 3.05e-14 | 65.1 |
| MS.gene84842.t1 | AT2G28290 | 47.458 | 59 | 31 | 0 | 1 | 59 | 790 | 848 | 3.05e-14 | 65.1 |
| MS.gene84842.t1 | AT3G12810 | 46.154 | 52 | 28 | 0 | 2 | 53 | 568 | 619 | 9.07e-12 | 58.2 |
| MS.gene84842.t1 | AT3G12810 | 46.154 | 52 | 28 | 0 | 2 | 53 | 572 | 623 | 9.07e-12 | 58.2 |
| MS.gene84842.t1 | AT3G06010 | 43.636 | 55 | 31 | 0 | 2 | 56 | 439 | 493 | 1.33e-11 | 57.8 |
Find 11 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTTAACCATGACAGCATT+TGG | 0.335190 | 8.4:-66216940 | None:intergenic |
| CAAATGCTGTCATGGTTAAC+TGG | 0.373905 | 8.4:+66216941 | MS.gene84842:CDS |
| GGCATTGATTGCATACTTGA+TGG | 0.376025 | 8.4:+66216879 | MS.gene84842:CDS |
| ATGGAATTCAAAGGAAACTA+TGG | 0.408379 | 8.4:+66216898 | MS.gene84842:CDS |
| AGTTAACCATGACAGCATTT+GGG | 0.429148 | 8.4:-66216939 | None:intergenic |
| TACAGAGTGAGTTATATAAG+TGG | 0.529773 | 8.4:+66217044 | MS.gene84842:intron |
| TTTGGGACTATTATGAGATG+TGG | 0.559813 | 8.4:-66216922 | None:intergenic |
| AATAGTCCCAAATGCTGTCA+TGG | 0.561516 | 8.4:+66216933 | MS.gene84842:CDS |
| GCATACTTGATGGAATTCAA+AGG | 0.579933 | 8.4:+66216889 | MS.gene84842:CDS |
| TAAAAGATGCACGACACAGA+TGG | 0.614890 | 8.4:-66217070 | None:intergenic |
| TGCTGTCATGGTTAACTGGA+AGG | 0.620544 | 8.4:+66216945 | MS.gene84842:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGGAATTCAAAGGAAACTA+TGG | + | chr8.4:66216898-66216917 | MS.gene84842:CDS | 30.0% | |
| TACAGAGTGAGTTATATAAG+TGG | + | chr8.4:66217044-66217063 | MS.gene84842:intron | 30.0% | |
| AGTTAACCATGACAGCATTT+GGG | - | chr8.4:66216942-66216961 | None:intergenic | 35.0% | |
| GCATACTTGATGGAATTCAA+AGG | + | chr8.4:66216889-66216908 | MS.gene84842:CDS | 35.0% | |
| ! | TTTGGGACTATTATGAGATG+TGG | - | chr8.4:66216925-66216944 | None:intergenic | 35.0% |
| AATAGTCCCAAATGCTGTCA+TGG | + | chr8.4:66216933-66216952 | MS.gene84842:CDS | 40.0% | |
| CAAATGCTGTCATGGTTAAC+TGG | + | chr8.4:66216941-66216960 | MS.gene84842:CDS | 40.0% | |
| CAGTTAACCATGACAGCATT+TGG | - | chr8.4:66216943-66216962 | None:intergenic | 40.0% | |
| TAAAAGATGCACGACACAGA+TGG | - | chr8.4:66217073-66217092 | None:intergenic | 40.0% | |
| !! | GGCATTGATTGCATACTTGA+TGG | + | chr8.4:66216879-66216898 | MS.gene84842:CDS | 40.0% |
| TGCTGTCATGGTTAACTGGA+AGG | + | chr8.4:66216945-66216964 | MS.gene84842:CDS | 45.0% | |
| ! | CATCTTTTATGCTGGAGGGA+AGG | + | chr8.4:66217084-66217103 | MS.gene84842:CDS | 45.0% |
| ! | CGTGCATCTTTTATGCTGGA+GGG | + | chr8.4:66217080-66217099 | MS.gene84842:CDS | 45.0% |
| ! | GTGTCGTGCATCTTTTATGC+TGG | + | chr8.4:66217076-66217095 | MS.gene84842:CDS | 45.0% |
| ! | TCGTGCATCTTTTATGCTGG+AGG | + | chr8.4:66217079-66217098 | MS.gene84842:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 66216874 | 66217132 | 66216874 | ID=MS.gene84842 |
| chr8.4 | mRNA | 66216874 | 66217132 | 66216874 | ID=MS.gene84842.t1;Parent=MS.gene84842 |
| chr8.4 | exon | 66216874 | 66216966 | 66216874 | ID=MS.gene84842.t1.exon1;Parent=MS.gene84842.t1 |
| chr8.4 | CDS | 66216874 | 66216966 | 66216874 | ID=cds.MS.gene84842.t1;Parent=MS.gene84842.t1 |
| chr8.4 | exon | 66217049 | 66217132 | 66217049 | ID=MS.gene84842.t1.exon2;Parent=MS.gene84842.t1 |
| chr8.4 | CDS | 66217049 | 66217132 | 66217049 | ID=cds.MS.gene84842.t1;Parent=MS.gene84842.t1 |
| Gene Sequence |
| Protein sequence |
