AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058647


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058647.t1 MTR_7g078090 98.039 51 1 0 1 51 1156 1206 1.38e-29 108
MS.gene058647.t1 MTR_8g030550 92.157 51 4 0 1 51 1148 1198 2.65e-19 79.0
MS.gene058647.t1 MTR_8g030550 92.157 51 4 0 1 51 1148 1198 2.87e-19 79.0
MS.gene058647.t1 MTR_8g030550 92.157 51 4 0 1 51 1148 1198 3.07e-19 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058647.t1 AT2G46020 82.353 51 9 0 1 51 1143 1193 5.17e-17 72.4
MS.gene058647.t1 AT2G46020 82.353 51 9 0 1 51 1143 1193 5.17e-17 72.4
MS.gene058647.t1 AT2G46020 82.353 51 9 0 1 51 1143 1193 5.17e-17 72.4
MS.gene058647.t1 AT2G46020 82.353 51 9 0 1 51 1142 1192 5.65e-17 72.4
MS.gene058647.t1 AT2G46020 82.353 51 9 0 1 51 1142 1192 5.65e-17 72.4
MS.gene058647.t1 AT2G46020 82.353 51 9 0 1 51 1142 1192 5.65e-17 72.4

Find 9 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAATCATCCTCTGCATTT+TGG 0.176846 7.2:+27682465 None:intergenic
ATAAGAAAGCCTTCAATGAT+TGG 0.353444 7.2:-27682520 MS.gene058647:CDS
ACCAAAATGCAGAGGATGAT+TGG 0.374727 7.2:-27682466 MS.gene058647:CDS
TTCTCTGCAGAATGATTTGA+AGG 0.385650 7.2:-27682588 None:intergenic
AGAATGATTTGAAGGAGCTT+TGG 0.417349 7.2:-27682580 MS.gene058647:CDS
GTTCTCGAAACCATTTCAAA+AGG 0.474139 7.2:-27682498 MS.gene058647:CDS
TTTCGAGAACCAATCATTGA+AGG 0.499720 7.2:+27682511 None:intergenic
TTTAAATCTACTTCTTCCTG+AGG 0.560138 7.2:-27682552 MS.gene058647:CDS
AGATCCCAACCAAAATGCAG+AGG 0.665916 7.2:-27682474 MS.gene058647:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATAAGAAAGCCTTCAATGAT+TGG - chr7.2:27682506-27682525 MS.gene058647:CDS 30.0%
TTCTTATTGTCAAAAACCTC+AGG + chr7.2:27682493-27682512 None:intergenic 30.0%
TTTAAATCTACTTCTTCCTG+AGG - chr7.2:27682474-27682493 MS.gene058647:CDS 30.0%
GTTCTCGAAACCATTTCAAA+AGG - chr7.2:27682528-27682547 MS.gene058647:CDS 35.0%
TTTCGAGAACCAATCATTGA+AGG + chr7.2:27682518-27682537 None:intergenic 35.0%
!! TTGGGATCTTCCTTTTGAAA+TGG + chr7.2:27682541-27682560 None:intergenic 35.0%
ACCAAAATGCAGAGGATGAT+TGG - chr7.2:27682560-27682579 MS.gene058647:CDS 40.0%
!! ATCATCCTCTGCATTTTGGT+TGG + chr7.2:27682560-27682579 None:intergenic 40.0%
!!! TCATCCTCTGCATTTTGGTT+GGG + chr7.2:27682559-27682578 None:intergenic 40.0%
AGATCCCAACCAAAATGCAG+AGG - chr7.2:27682552-27682571 MS.gene058647:CDS 45.0%
! GCCAATCATCCTCTGCATTT+TGG + chr7.2:27682564-27682583 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 27682448 27682600 27682448 ID=MS.gene058647
chr7.2 mRNA 27682448 27682600 27682448 ID=MS.gene058647.t1;Parent=MS.gene058647
chr7.2 exon 27682448 27682600 27682448 ID=MS.gene058647.t1.exon1;Parent=MS.gene058647.t1
chr7.2 CDS 27682448 27682600 27682448 ID=cds.MS.gene058647.t1;Parent=MS.gene058647.t1
Gene Sequence

>MS.gene058647

AATGATTTGAAGGAGCTTTGGTCACTTTTAAATCTACTTCTTCCTGAGGTTTTTGACAATAAGAAAGCCTTCAATGATTGGTTCTCGAAACCATTTCAAAAGGAAGATCCCAACCAAAATGCAGAGGATGATTGGCTTGAGACAGAGAAGAAA

Protein sequence

>MS.gene058647.t1

NDLKELWSLLNLLLPEVFDNKKAFNDWFSKPFQKEDPNQNAEDDWLETEKK