Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84862.t1 | XP_024629354.1 | 95.1 | 425 | 19 | 1 | 1 | 423 | 59 | 483 | 7.90E-229 | 802.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84862.t1 | F4IJ77 | 57.5 | 428 | 161 | 7 | 1 | 413 | 60 | 481 | 1.5e-130 | 467.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84862.t1 | A0A396GJ98 | 95.1 | 425 | 19 | 1 | 1 | 423 | 59 | 483 | 5.7e-229 | 802.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060518 | MS.gene84862 | 0.95683 | 1.17E-114 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84862.t1 | MTR_8g027200 | 89.412 | 425 | 17 | 2 | 1 | 423 | 59 | 457 | 0.0 | 759 |
| MS.gene84862.t1 | MTR_7g117950 | 63.614 | 415 | 127 | 9 | 1 | 414 | 61 | 452 | 0.0 | 522 |
| MS.gene84862.t1 | MTR_3g092060 | 52.745 | 419 | 174 | 9 | 4 | 412 | 66 | 470 | 9.43e-154 | 444 |
| MS.gene84862.t1 | MTR_2g061820 | 36.667 | 390 | 211 | 5 | 1 | 356 | 57 | 444 | 9.79e-87 | 273 |
| MS.gene84862.t1 | MTR_4g128260 | 33.810 | 420 | 225 | 8 | 1 | 371 | 59 | 474 | 1.11e-71 | 234 |
| MS.gene84862.t1 | MTR_8g076920 | 35.635 | 362 | 200 | 9 | 4 | 338 | 81 | 436 | 1.21e-67 | 223 |
| MS.gene84862.t1 | MTR_5g020980 | 35.393 | 356 | 199 | 5 | 34 | 360 | 100 | 453 | 8.69e-66 | 218 |
| MS.gene84862.t1 | MTR_1g022250 | 34.010 | 394 | 207 | 10 | 7 | 357 | 69 | 452 | 1.83e-65 | 217 |
| MS.gene84862.t1 | MTR_2g076690 | 36.257 | 342 | 182 | 10 | 41 | 356 | 104 | 435 | 2.91e-65 | 216 |
| MS.gene84862.t1 | MTR_2g030420 | 33.760 | 391 | 207 | 9 | 7 | 357 | 72 | 450 | 3.36e-65 | 216 |
| MS.gene84862.t1 | MTR_6g009720 | 33.947 | 380 | 196 | 8 | 7 | 335 | 71 | 446 | 1.06e-64 | 216 |
| MS.gene84862.t1 | MTR_2g076840 | 32.199 | 382 | 220 | 10 | 1 | 356 | 58 | 426 | 5.55e-64 | 218 |
| MS.gene84862.t1 | MTR_2g076840 | 41.532 | 248 | 130 | 6 | 122 | 357 | 460 | 704 | 1.78e-57 | 201 |
| MS.gene84862.t1 | MTR_2g076970 | 36.913 | 298 | 167 | 6 | 70 | 356 | 141 | 428 | 2.44e-63 | 211 |
| MS.gene84862.t1 | MTR_7g008040 | 34.133 | 375 | 197 | 9 | 29 | 356 | 89 | 460 | 2.80e-63 | 213 |
| MS.gene84862.t1 | MTR_4g107370 | 33.245 | 379 | 196 | 8 | 31 | 355 | 95 | 470 | 3.10e-63 | 212 |
| MS.gene84862.t1 | MTR_1g022245 | 33.903 | 351 | 180 | 7 | 34 | 335 | 91 | 438 | 3.63e-63 | 211 |
| MS.gene84862.t1 | MTR_6g009520 | 32.152 | 395 | 221 | 10 | 7 | 357 | 71 | 462 | 3.70e-63 | 212 |
| MS.gene84862.t1 | MTR_6g009553 | 31.941 | 407 | 217 | 9 | 7 | 357 | 47 | 449 | 9.86e-63 | 210 |
| MS.gene84862.t1 | MTR_6g009770 | 31.852 | 405 | 218 | 9 | 7 | 357 | 48 | 448 | 1.02e-62 | 210 |
| MS.gene84862.t1 | MTR_6g009540 | 31.671 | 401 | 227 | 9 | 1 | 357 | 63 | 460 | 1.46e-62 | 210 |
| MS.gene84862.t1 | MTR_5g020990 | 33.161 | 386 | 202 | 10 | 17 | 356 | 95 | 470 | 1.68e-62 | 209 |
| MS.gene84862.t1 | MTR_2g076710 | 34.018 | 341 | 196 | 8 | 41 | 356 | 102 | 438 | 4.11e-62 | 207 |
| MS.gene84862.t1 | MTR_2g076920 | 34.188 | 351 | 192 | 9 | 33 | 356 | 90 | 428 | 4.79e-62 | 208 |
| MS.gene84862.t1 | MTR_2g076900 | 31.070 | 383 | 230 | 9 | 1 | 356 | 58 | 433 | 2.66e-60 | 203 |
| MS.gene84862.t1 | MTR_2g076990 | 29.197 | 411 | 238 | 10 | 1 | 382 | 1 | 387 | 2.06e-59 | 200 |
| MS.gene84862.t1 | MTR_6g042290 | 34.091 | 352 | 205 | 8 | 7 | 335 | 81 | 428 | 3.53e-59 | 201 |
| MS.gene84862.t1 | MTR_3g103150 | 39.357 | 249 | 134 | 5 | 122 | 356 | 31 | 276 | 1.41e-57 | 191 |
| MS.gene84862.t1 | MTR_6g009510 | 31.720 | 372 | 198 | 9 | 1 | 356 | 75 | 406 | 2.79e-54 | 187 |
| MS.gene84862.t1 | MTR_6g009660 | 28.686 | 373 | 192 | 10 | 29 | 357 | 70 | 412 | 3.12e-44 | 160 |
| MS.gene84862.t1 | MTR_5g021020 | 31.861 | 317 | 171 | 13 | 56 | 356 | 118 | 405 | 2.25e-39 | 146 |
| MS.gene84862.t1 | MTR_5g020720 | 36.986 | 146 | 79 | 3 | 191 | 324 | 25 | 169 | 6.71e-21 | 90.5 |
| MS.gene84862.t1 | MTR_5g021000 | 38.889 | 108 | 61 | 4 | 251 | 356 | 1 | 105 | 7.85e-18 | 80.9 |
| MS.gene84862.t1 | MTR_1g011680 | 35.417 | 96 | 60 | 2 | 240 | 335 | 18 | 111 | 1.49e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84862.t1 | AT2G46620 | 57.437 | 437 | 165 | 7 | 1 | 423 | 60 | 489 | 1.32e-164 | 472 |
| MS.gene84862.t1 | AT4G25835 | 35.581 | 430 | 221 | 9 | 1 | 384 | 59 | 478 | 3.18e-76 | 246 |
| MS.gene84862.t1 | AT5G57480 | 35.202 | 446 | 218 | 10 | 4 | 399 | 62 | 486 | 6.47e-75 | 243 |
| MS.gene84862.t1 | AT5G17760 | 41.776 | 304 | 147 | 8 | 71 | 356 | 163 | 454 | 3.00e-69 | 228 |
| MS.gene84862.t1 | AT3G28580 | 35.897 | 351 | 181 | 7 | 30 | 338 | 91 | 439 | 5.55e-68 | 224 |
| MS.gene84862.t1 | AT4G30250 | 34.750 | 400 | 212 | 8 | 4 | 356 | 62 | 459 | 2.35e-67 | 223 |
| MS.gene84862.t1 | AT4G30250 | 34.750 | 400 | 212 | 8 | 4 | 356 | 64 | 461 | 2.41e-67 | 223 |
| MS.gene84862.t1 | AT5G40010 | 35.230 | 369 | 189 | 9 | 26 | 348 | 90 | 454 | 4.78e-67 | 222 |
| MS.gene84862.t1 | AT3G50930 | 36.212 | 359 | 188 | 10 | 34 | 357 | 148 | 500 | 5.48e-67 | 223 |
| MS.gene84862.t1 | AT2G18193 | 38.623 | 334 | 174 | 8 | 34 | 343 | 101 | 427 | 8.32e-67 | 221 |
| MS.gene84862.t1 | AT3G28610 | 37.425 | 334 | 175 | 10 | 37 | 343 | 106 | 432 | 1.66e-65 | 217 |
| MS.gene84862.t1 | AT3G28580 | 36.280 | 328 | 189 | 6 | 30 | 338 | 91 | 417 | 2.14e-65 | 217 |
| MS.gene84862.t1 | AT3G28600 | 37.176 | 347 | 184 | 11 | 37 | 356 | 107 | 446 | 2.78e-65 | 216 |
| MS.gene84862.t1 | AT3G50940 | 35.758 | 330 | 180 | 7 | 34 | 338 | 102 | 424 | 9.98e-65 | 214 |
| MS.gene84862.t1 | AT5G17750 | 36.364 | 341 | 179 | 7 | 13 | 322 | 57 | 390 | 2.07e-64 | 212 |
| MS.gene84862.t1 | AT2G18190 | 36.474 | 329 | 181 | 7 | 34 | 338 | 102 | 426 | 1.08e-63 | 213 |
| MS.gene84862.t1 | AT5G17740 | 31.765 | 425 | 230 | 13 | 3 | 390 | 71 | 472 | 2.51e-63 | 213 |
| MS.gene84862.t1 | AT5G17730 | 37.374 | 297 | 165 | 7 | 73 | 356 | 155 | 443 | 2.65e-63 | 211 |
| MS.gene84862.t1 | AT3G28510 | 33.062 | 369 | 197 | 8 | 33 | 356 | 95 | 458 | 2.93e-61 | 207 |
| MS.gene84862.t1 | AT3G28540 | 34.012 | 344 | 181 | 8 | 33 | 335 | 96 | 434 | 3.01e-61 | 207 |
| MS.gene84862.t1 | AT3G28540 | 34.012 | 344 | 181 | 8 | 33 | 335 | 96 | 434 | 3.79e-61 | 206 |
| MS.gene84862.t1 | AT5G40000 | 35.652 | 345 | 191 | 11 | 37 | 356 | 111 | 449 | 5.40e-61 | 205 |
| MS.gene84862.t1 | AT4G05380 | 37.500 | 336 | 176 | 8 | 47 | 356 | 120 | 447 | 1.83e-60 | 203 |
| MS.gene84862.t1 | AT3G28600 | 39.928 | 278 | 140 | 7 | 104 | 356 | 132 | 407 | 1.20e-59 | 201 |
| MS.gene84862.t1 | AT3G28570 | 33.523 | 352 | 211 | 9 | 22 | 356 | 88 | 433 | 3.78e-59 | 200 |
| MS.gene84862.t1 | AT3G28570 | 33.523 | 352 | 211 | 9 | 22 | 356 | 95 | 440 | 4.73e-59 | 199 |
| MS.gene84862.t1 | AT1G43910 | 34.259 | 324 | 179 | 7 | 47 | 343 | 119 | 435 | 2.33e-53 | 185 |
| MS.gene84862.t1 | AT3G28520 | 32.945 | 343 | 188 | 8 | 33 | 338 | 85 | 422 | 4.86e-52 | 181 |
| MS.gene84862.t1 | AT5G17760 | 48.428 | 159 | 75 | 2 | 71 | 229 | 163 | 314 | 2.02e-40 | 147 |
| MS.gene84862.t1 | AT3G29800 | 28.875 | 329 | 205 | 11 | 27 | 342 | 55 | 367 | 9.88e-37 | 139 |
| MS.gene84862.t1 | AT4G05340 | 47.826 | 69 | 35 | 1 | 242 | 310 | 27 | 94 | 2.10e-13 | 66.2 |
Find 97 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATGGTTTGTTTAGATTA+AGG | 0.215457 | 8.4:-69780935 | MS.gene84862:CDS |
| ACGGATTTGAGGAAGATTTA+TGG | 0.228137 | 8.4:-69780951 | MS.gene84862:CDS |
| TCGGTTTATAACATCGGATT+TGG | 0.271342 | 8.4:-69781444 | MS.gene84862:CDS |
| TTCTCGGCGCCACGGTTTAT+TGG | 0.279842 | 8.4:-69781979 | MS.gene84862:CDS |
| GAATAGGTCGGGTAATTTAT+TGG | 0.293961 | 8.4:-69780901 | MS.gene84862:CDS |
| GCTGAGTATTCTGCGTTTAT+CGG | 0.300204 | 8.4:+69781898 | None:intergenic |
| GGGGTGGTGGAAGGAGGTGG+AGG | 0.315568 | 8.4:-69781784 | MS.gene84862:CDS |
| ACAAATGCACCTGTTTGATT+TGG | 0.324681 | 8.4:+69781938 | None:intergenic |
| AACATTTGAAACGATGTTGA+TGG | 0.338402 | 8.4:-69781738 | MS.gene84862:CDS |
| AATGTTGATGGATGATTAAA+CGG | 0.346660 | 8.4:+69781755 | None:intergenic |
| TAAGGAATGTTAAGAGGAAT+AGG | 0.348823 | 8.4:-69780917 | MS.gene84862:CDS |
| CCGGCAACGGTTACGGCGGC+GGG | 0.352366 | 8.4:-69781416 | MS.gene84862:CDS |
| AAACGGAGCTTCTTGTTATA+CGG | 0.355478 | 8.4:-69781629 | MS.gene84862:CDS |
| ATAAAAGAATCGGTCGTGTT+TGG | 0.378794 | 8.4:-69781652 | MS.gene84862:CDS |
| TGCACCTGTTTGATTTGGTT+CGG | 0.381073 | 8.4:+69781943 | None:intergenic |
| GACTTCACCAATCTCATTAC+CGG | 0.382732 | 8.4:-69782061 | MS.gene84862:CDS |
| GAAAATCATTATTCTTTCTT+CGG | 0.384048 | 8.4:+69781352 | None:intergenic |
| ATTATTTCCGGTAATGAGAT+TGG | 0.397632 | 8.4:+69782054 | None:intergenic |
| CGGGCTATTAAATCGGTAAT+CGG | 0.398376 | 8.4:-69781095 | MS.gene84862:CDS |
| CCCGCCGCCGTAACCGTTGC+CGG | 0.398549 | 8.4:+69781416 | None:intergenic |
| TCCGGCAACGGTTACGGCGG+CGG | 0.415110 | 8.4:-69781417 | MS.gene84862:CDS |
| CGACCAGGTCGGAGAAAATT+CGG | 0.415681 | 8.4:+69781302 | None:intergenic |
| AACATCGTTTCAAATGTTGA+TGG | 0.416178 | 8.4:+69781743 | None:intergenic |
| TTCGTTAATATCACCGGCGG+TGG | 0.419095 | 8.4:-69781806 | MS.gene84862:CDS |
| GGTAATCGGGGCATTGCAAA+TGG | 0.420117 | 8.4:-69781081 | MS.gene84862:CDS |
| GGAATCTCCGGCAACGGTTA+CGG | 0.421241 | 8.4:-69781423 | MS.gene84862:CDS |
| GTTAATATCACCGGCGGTGG+GGG | 0.422663 | 8.4:-69781803 | MS.gene84862:CDS |
| TCAACAACGATAATAGATTT+CGG | 0.429950 | 8.4:+69781476 | None:intergenic |
| TTCTTGTTATACGGTGAATC+CGG | 0.433520 | 8.4:-69781620 | MS.gene84862:CDS |
| GGGCTATTAAATCGGTAATC+GGG | 0.437429 | 8.4:-69781094 | MS.gene84862:CDS |
| AACATCGGATTTGGAATCTC+CGG | 0.444407 | 8.4:-69781435 | MS.gene84862:CDS |
| TTAGATTAAGGAATGTTAAG+AGG | 0.444604 | 8.4:-69780923 | MS.gene84862:CDS |
| GGTGTTCTTTGTGGTGGTGA+TGG | 0.454004 | 8.4:-69780981 | MS.gene84862:CDS |
| AGTTTGAGTCCGGCGGAGAT+AGG | 0.458254 | 8.4:-69781152 | MS.gene84862:CDS |
| CGGAAAATCTCCTCCACCGC+CGG | 0.462392 | 8.4:+69781185 | None:intergenic |
| GAATCGGTCGTGTTTGGAAA+CGG | 0.462731 | 8.4:-69781646 | MS.gene84862:CDS |
| ATCATCGAAGACAGGTTTCT+CGG | 0.463592 | 8.4:-69781995 | MS.gene84862:CDS |
| GACGGTGATGGAAAAGGGTG+TGG | 0.465085 | 8.4:-69781059 | MS.gene84862:CDS |
| AAGAATTATTATAAAAGAAT+CGG | 0.466313 | 8.4:-69781662 | MS.gene84862:CDS |
| GCCGAGTCGGGCTATTAAAT+CGG | 0.467127 | 8.4:-69781102 | MS.gene84862:CDS |
| GACTCAAACTCGCTCCCTGC+CGG | 0.469388 | 8.4:+69781165 | None:intergenic |
| TCGTTAATATCACCGGCGGT+GGG | 0.469999 | 8.4:-69781805 | MS.gene84862:CDS |
| TATGATCGTACGGCGGATAG+AGG | 0.472611 | 8.4:-69781033 | MS.gene84862:CDS |
| ACTTTACGATAAAACATGTT+CGG | 0.478150 | 8.4:+69782124 | None:intergenic |
| ATCTAGCTTTGTTGCAGCCA+TGG | 0.479264 | 8.4:-69781588 | MS.gene84862:CDS |
| GAATCGGAACTCGCCGAGTC+GGG | 0.479389 | 8.4:-69781114 | MS.gene84862:CDS |
| AATTTATTGGTCAATGATGA+AGG | 0.489531 | 8.4:-69780888 | MS.gene84862:CDS |
| GCTTTGGCGAATAGTTACTT+AGG | 0.495433 | 8.4:-69781230 | MS.gene84862:CDS |
| ATAAAACATGTTCGGTTGCA+TGG | 0.503776 | 8.4:+69782132 | None:intergenic |
| CGTTAATATCACCGGCGGTG+GGG | 0.505377 | 8.4:-69781804 | MS.gene84862:CDS |
| AAAACCGAACCAAATCAAAC+AGG | 0.507238 | 8.4:-69781947 | MS.gene84862:CDS |
| ACGGTGATGGAAAAGGGTGT+GGG | 0.516936 | 8.4:-69781058 | MS.gene84862:CDS |
| GGCGGGGATTCAGAATTTCA+TGG | 0.525375 | 8.4:-69781399 | MS.gene84862:CDS |
| ATATGAACGTCCACACGACC+AGG | 0.525421 | 8.4:+69781287 | None:intergenic |
| GCAGGGAGCGAGTTTGAGTC+CGG | 0.529877 | 8.4:-69781162 | MS.gene84862:CDS |
| CGTTTGTTCGTTAATATCAC+CGG | 0.539498 | 8.4:-69781812 | MS.gene84862:CDS |
| GAAATCTATTATCGTTGTTG+AGG | 0.540126 | 8.4:-69781474 | MS.gene84862:CDS |
| GAATGTTAAGAGGAATAGGT+CGG | 0.544625 | 8.4:-69780913 | MS.gene84862:CDS |
| GTGAGTTGATGATTGCGAAT+CGG | 0.552462 | 8.4:-69781130 | MS.gene84862:CDS |
| CGAATCGGAACTCGCCGAGT+CGG | 0.559326 | 8.4:-69781115 | MS.gene84862:CDS |
| GCCGTTGTATTCTATTTCAG+CGG | 0.560252 | 8.4:+69781847 | None:intergenic |
| CCGATCAAATCATCGAAGAC+AGG | 0.562107 | 8.4:-69782003 | MS.gene84862:CDS |
| GTATTCTATTTCAGCGGACA+TGG | 0.562971 | 8.4:+69781853 | None:intergenic |
| CGGTGATGGAAAAGGGTGTG+GGG | 0.563898 | 8.4:-69781057 | MS.gene84862:CDS |
| AATGTTAAGAGGAATAGGTC+GGG | 0.568612 | 8.4:-69780912 | MS.gene84862:CDS |
| CCTGTCTTCGATGATTTGAT+CGG | 0.569361 | 8.4:+69782003 | None:intergenic |
| CAAATGGACGGTGATGGAAA+AGG | 0.573028 | 8.4:-69781065 | MS.gene84862:CDS |
| ATCAACTCACCTATCTCCGC+CGG | 0.583450 | 8.4:+69781143 | None:intergenic |
| TTATACGGTGAATCCGGTAC+CGG | 0.585271 | 8.4:-69781614 | MS.gene84862:CDS |
| AGACAGGTTTCTCGGCGCCA+CGG | 0.586250 | 8.4:-69781987 | MS.gene84862:CDS |
| TCTCGATCGGTTTATAACAT+CGG | 0.589482 | 8.4:-69781450 | MS.gene84862:CDS |
| AGTAACCAAGGTGTTCTTTG+TGG | 0.595481 | 8.4:-69780990 | MS.gene84862:CDS |
| GGATTTGGAATCTCCGGCAA+CGG | 0.595822 | 8.4:-69781429 | MS.gene84862:CDS |
| GCATTGCAAATGGACGGTGA+TGG | 0.605343 | 8.4:-69781071 | MS.gene84862:CDS |
| GGCTATTAAATCGGTAATCG+GGG | 0.605421 | 8.4:-69781093 | MS.gene84862:CDS |
| ATCGGGGCATTGCAAATGGA+CGG | 0.606342 | 8.4:-69781077 | MS.gene84862:CDS |
| AAGTCGGAATCTTCAAGAGA+AGG | 0.608105 | 8.4:+69782079 | None:intergenic |
| AAATGGACGGTGATGGAAAA+GGG | 0.611545 | 8.4:-69781064 | MS.gene84862:CDS |
| ATCTCCGGCAACGGTTACGG+CGG | 0.614797 | 8.4:-69781420 | MS.gene84862:CDS |
| GGTAATGAGATTGGTGAAGT+CGG | 0.621262 | 8.4:+69782063 | None:intergenic |
| GGGATTCAGAATTTCATGGA+CGG | 0.622282 | 8.4:-69781395 | MS.gene84862:CDS |
| TTTATAGAACCAATAAACCG+TGG | 0.631666 | 8.4:+69781970 | None:intergenic |
| ACCGATTTAATAGCCCGACT+CGG | 0.633107 | 8.4:+69781101 | None:intergenic |
| GAACGTCCACACGACCAGGT+CGG | 0.633799 | 8.4:+69781291 | None:intergenic |
| AACCAAGGTGTTCTTTGTGG+TGG | 0.636999 | 8.4:-69780987 | MS.gene84862:CDS |
| CGGCAACGGTTACGGCGGCG+GGG | 0.642529 | 8.4:-69781415 | MS.gene84862:CDS |
| GGTGTGGGGATATGATCGTA+CGG | 0.644792 | 8.4:-69781043 | MS.gene84862:CDS |
| TCGTTGTTGAGGATCTCGAT+CGG | 0.655691 | 8.4:-69781463 | MS.gene84862:CDS |
| ATAACAAAGAAAATTCGCCA+TGG | 0.656962 | 8.4:+69781571 | None:intergenic |
| GAATAGTTACTTAGGTGTGA+AGG | 0.666896 | 8.4:-69781222 | MS.gene84862:CDS |
| TTGTTCGTTAATATCACCGG+CGG | 0.670649 | 8.4:-69781809 | MS.gene84862:CDS |
| CACCACCACAAAGAACACCT+TGG | 0.671779 | 8.4:+69780985 | None:intergenic |
| GCGGATAGAGGATGATGACG+TGG | 0.692126 | 8.4:-69781021 | MS.gene84862:CDS |
| GTGGAAGATGAAAGTAACCA+AGG | 0.702124 | 8.4:-69781002 | MS.gene84862:CDS |
| GGGAGCGAGTTTGAGTCCGG+CGG | 0.703907 | 8.4:-69781159 | MS.gene84862:CDS |
| TCCGCTGAAATAGAATACAA+CGG | 0.706310 | 8.4:-69781848 | MS.gene84862:CDS |
| GTGGGGATATGATCGTACGG+CGG | 0.779328 | 8.4:-69781040 | MS.gene84862:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAATTATTATAAAAGAAT+CGG | - | chr8.4:69781349-69781368 | MS.gene84862:CDS | 10.0% |
| !! | GAAAATCATTATTCTTTCTT+CGG | + | chr8.4:69781662-69781681 | None:intergenic | 20.0% |
| !!! | TTTATGGTTTGTTTAGATTA+AGG | - | chr8.4:69782076-69782095 | MS.gene84862:CDS | 20.0% |
| !!! | TTTTTCATCTTTTAAAGCTT+TGG | - | chr8.4:69781765-69781784 | MS.gene84862:CDS | 20.0% |
| ! | AATTTATTGGTCAATGATGA+AGG | - | chr8.4:69782123-69782142 | MS.gene84862:CDS | 25.0% |
| ! | ACTTTACGATAAAACATGTT+CGG | + | chr8.4:69780890-69780909 | None:intergenic | 25.0% |
| ! | TCAACAACGATAATAGATTT+CGG | + | chr8.4:69781538-69781557 | None:intergenic | 25.0% |
| ! | TTAGATTAAGGAATGTTAAG+AGG | - | chr8.4:69782088-69782107 | MS.gene84862:CDS | 25.0% |
| !! | AATGTTGATGGATGATTAAA+CGG | + | chr8.4:69781259-69781278 | None:intergenic | 25.0% |
| AAAGATGAAAAATCACACAC+CGG | + | chr8.4:69781757-69781776 | None:intergenic | 30.0% | |
| AACATCGTTTCAAATGTTGA+TGG | + | chr8.4:69781271-69781290 | None:intergenic | 30.0% | |
| ATAACAAAGAAAATTCGCCA+TGG | + | chr8.4:69781443-69781462 | None:intergenic | 30.0% | |
| ATTATTTCCGGTAATGAGAT+TGG | + | chr8.4:69780960-69780979 | None:intergenic | 30.0% | |
| GAAATCTATTATCGTTGTTG+AGG | - | chr8.4:69781537-69781556 | MS.gene84862:CDS | 30.0% | |
| TAAGGAATGTTAAGAGGAAT+AGG | - | chr8.4:69782094-69782113 | MS.gene84862:CDS | 30.0% | |
| TTTATAGAACCAATAAACCG+TGG | + | chr8.4:69781044-69781063 | None:intergenic | 30.0% | |
| ! | AACATTTGAAACGATGTTGA+TGG | - | chr8.4:69781273-69781292 | MS.gene84862:CDS | 30.0% |
| !!! | GATGTCGTTTTGATTATTTC+CGG | + | chr8.4:69780972-69780991 | None:intergenic | 30.0% |
| AAAACCGAACCAAATCAAAC+AGG | - | chr8.4:69781064-69781083 | MS.gene84862:CDS | 35.0% | |
| AATGTTAAGAGGAATAGGTC+GGG | - | chr8.4:69782099-69782118 | MS.gene84862:CDS | 35.0% | |
| ACAAATGCACCTGTTTGATT+TGG | + | chr8.4:69781076-69781095 | None:intergenic | 35.0% | |
| ACGGATTTGAGGAAGATTTA+TGG | - | chr8.4:69782060-69782079 | MS.gene84862:CDS | 35.0% | |
| ATAAAAGAATCGGTCGTGTT+TGG | - | chr8.4:69781359-69781378 | MS.gene84862:CDS | 35.0% | |
| CGTTTGTTCGTTAATATCAC+CGG | - | chr8.4:69781199-69781218 | MS.gene84862:CDS | 35.0% | |
| GAATAGGTCGGGTAATTTAT+TGG | - | chr8.4:69782110-69782129 | MS.gene84862:CDS | 35.0% | |
| GAATAGTTACTTAGGTGTGA+AGG | - | chr8.4:69781789-69781808 | MS.gene84862:CDS | 35.0% | |
| GAATGTTAAGAGGAATAGGT+CGG | - | chr8.4:69782098-69782117 | MS.gene84862:CDS | 35.0% | |
| TCCGCTGAAATAGAATACAA+CGG | - | chr8.4:69781163-69781182 | MS.gene84862:CDS | 35.0% | |
| TCGGTTTATAACATCGGATT+TGG | - | chr8.4:69781567-69781586 | MS.gene84862:CDS | 35.0% | |
| ! | AAACGGAGCTTCTTGTTATA+CGG | - | chr8.4:69781382-69781401 | MS.gene84862:CDS | 35.0% |
| ! | ATGGTTTTTCTACGGATTTG+AGG | - | chr8.4:69782049-69782068 | MS.gene84862:CDS | 35.0% |
| ! | GGACGTTCATATTCATTTTC+CGG | - | chr8.4:69781735-69781754 | MS.gene84862:CDS | 35.0% |
| ! | TCTCGATCGGTTTATAACAT+CGG | - | chr8.4:69781561-69781580 | MS.gene84862:CDS | 35.0% |
| ! | TGCAACAAAGCTAGATTTTC+CGG | + | chr8.4:69781419-69781438 | None:intergenic | 35.0% |
| ! | TTCTTGTTATACGGTGAATC+CGG | - | chr8.4:69781391-69781410 | MS.gene84862:CDS | 35.0% |
| AAGTCGGAATCTTCAAGAGA+AGG | + | chr8.4:69780935-69780954 | None:intergenic | 40.0% | |
| ATCATCGAAGACAGGTTTCT+CGG | - | chr8.4:69781016-69781035 | MS.gene84862:CDS | 40.0% | |
| CCTGTCTTCGATGATTTGAT+CGG | + | chr8.4:69781011-69781030 | None:intergenic | 40.0% | |
| CGGGCTATTAAATCGGTAAT+CGG | - | chr8.4:69781916-69781935 | MS.gene84862:CDS | 40.0% | |
| GACTTCACCAATCTCATTAC+CGG | - | chr8.4:69780950-69780969 | MS.gene84862:CDS | 40.0% | |
| GCCGTTGTATTCTATTTCAG+CGG | + | chr8.4:69781167-69781186 | None:intergenic | 40.0% | |
| GCTGAGTATTCTGCGTTTAT+CGG | + | chr8.4:69781116-69781135 | None:intergenic | 40.0% | |
| GCTTTGGCGAATAGTTACTT+AGG | - | chr8.4:69781781-69781800 | MS.gene84862:CDS | 40.0% | |
| GGCTATTAAATCGGTAATCG+GGG | - | chr8.4:69781918-69781937 | MS.gene84862:CDS | 40.0% | |
| GGGATTCAGAATTTCATGGA+CGG | - | chr8.4:69781616-69781635 | MS.gene84862:CDS | 40.0% | |
| GGGCTATTAAATCGGTAATC+GGG | - | chr8.4:69781917-69781936 | MS.gene84862:CDS | 40.0% | |
| GGTAATGAGATTGGTGAAGT+CGG | + | chr8.4:69780951-69780970 | None:intergenic | 40.0% | |
| GTATTCTATTTCAGCGGACA+TGG | + | chr8.4:69781161-69781180 | None:intergenic | 40.0% | |
| GTGGAAGATGAAAGTAACCA+AGG | - | chr8.4:69782009-69782028 | MS.gene84862:CDS | 40.0% | |
| TTGTTCGTTAATATCACCGG+CGG | - | chr8.4:69781202-69781221 | MS.gene84862:CDS | 40.0% | |
| ! | AAAGCTAGATTTTCCGGTAC+CGG | + | chr8.4:69781413-69781432 | None:intergenic | 40.0% |
| ! | AACATCGGATTTGGAATCTC+CGG | - | chr8.4:69781576-69781595 | MS.gene84862:CDS | 40.0% |
| ! | AGTAACCAAGGTGTTCTTTG+TGG | - | chr8.4:69782021-69782040 | MS.gene84862:CDS | 40.0% |
| !! | AAATGGACGGTGATGGAAAA+GGG | - | chr8.4:69781947-69781966 | MS.gene84862:CDS | 40.0% |
| !! | GAAGGAACACAAGCTTTTTC+CGG | - | chr8.4:69781807-69781826 | MS.gene84862:CDS | 40.0% |
| !! | GTGAGTTGATGATTGCGAAT+CGG | - | chr8.4:69781881-69781900 | MS.gene84862:CDS | 40.0% |
| !! | TGCACCTGTTTGATTTGGTT+CGG | + | chr8.4:69781071-69781090 | None:intergenic | 40.0% |
| !!! | TGGTGGTGATGGTTTTTCTA+CGG | - | chr8.4:69782041-69782060 | MS.gene84862:CDS | 40.0% |
| CCGATCAAATCATCGAAGAC+AGG | - | chr8.4:69781008-69781027 | MS.gene84862:CDS | 45.0% | |
| GAATCGGTCGTGTTTGGAAA+CGG | - | chr8.4:69781365-69781384 | MS.gene84862:CDS | 45.0% | |
| TCGTTGTTGAGGATCTCGAT+CGG | - | chr8.4:69781548-69781567 | MS.gene84862:CDS | 45.0% | |
| TTATACGGTGAATCCGGTAC+CGG | - | chr8.4:69781397-69781416 | MS.gene84862:CDS | 45.0% | |
| ! | AACCAAGGTGTTCTTTGTGG+TGG | - | chr8.4:69782024-69782043 | MS.gene84862:CDS | 45.0% |
| ! | ACCGATTTAATAGCCCGACT+CGG | + | chr8.4:69781913-69781932 | None:intergenic | 45.0% |
| !! | ATCTAGCTTTGTTGCAGCCA+TGG | - | chr8.4:69781423-69781442 | MS.gene84862:CDS | 45.0% |
| !! | CAAATGGACGGTGATGGAAA+AGG | - | chr8.4:69781946-69781965 | MS.gene84862:CDS | 45.0% |
| ATATGAACGTCCACACGACC+AGG | + | chr8.4:69781727-69781746 | None:intergenic | 50.0% | |
| ATCAACTCACCTATCTCCGC+CGG | + | chr8.4:69781871-69781890 | None:intergenic | 50.0% | |
| CACCACCACAAAGAACACCT+TGG | + | chr8.4:69782029-69782048 | None:intergenic | 50.0% | |
| CGACCAGGTCGGAGAAAATT+CGG | + | chr8.4:69781712-69781731 | None:intergenic | 50.0% | |
| GCATTGCAAATGGACGGTGA+TGG | - | chr8.4:69781940-69781959 | MS.gene84862:CDS | 50.0% | |
| GCCGAGTCGGGCTATTAAAT+CGG | - | chr8.4:69781909-69781928 | MS.gene84862:CDS | 50.0% | |
| GGCGGGGATTCAGAATTTCA+TGG | - | chr8.4:69781612-69781631 | MS.gene84862:CDS | 50.0% | |
| GGTGTGGGGATATGATCGTA+CGG | - | chr8.4:69781968-69781987 | MS.gene84862:CDS | 50.0% | |
| TATGATCGTACGGCGGATAG+AGG | - | chr8.4:69781978-69781997 | MS.gene84862:CDS | 50.0% | |
| TCGTTAATATCACCGGCGGT+GGG | - | chr8.4:69781206-69781225 | MS.gene84862:CDS | 50.0% | |
| TTCGTTAATATCACCGGCGG+TGG | - | chr8.4:69781205-69781224 | MS.gene84862:CDS | 50.0% | |
| ! | GATCCGAATTTTCTCCGACC+TGG | - | chr8.4:69781706-69781725 | MS.gene84862:CDS | 50.0% |
| ! | GGATTTGGAATCTCCGGCAA+CGG | - | chr8.4:69781582-69781601 | MS.gene84862:CDS | 50.0% |
| ! | GGTGTTCTTTGTGGTGGTGA+TGG | - | chr8.4:69782030-69782049 | MS.gene84862:CDS | 50.0% |
| !! | ACGGTGATGGAAAAGGGTGT+GGG | - | chr8.4:69781953-69781972 | MS.gene84862:CDS | 50.0% |
| !! | ATCGGGGCATTGCAAATGGA+CGG | - | chr8.4:69781934-69781953 | MS.gene84862:CDS | 50.0% |
| !! | GGAACACAAGCTTTTTCCGG+CGG | - | chr8.4:69781810-69781829 | MS.gene84862:CDS | 50.0% |
| !! | GGTAATCGGGGCATTGCAAA+TGG | - | chr8.4:69781930-69781949 | MS.gene84862:CDS | 50.0% |
| AGTTTGAGTCCGGCGGAGAT+AGG | - | chr8.4:69781859-69781878 | MS.gene84862:CDS | 55.0% | |
| CGTTAATATCACCGGCGGTG+GGG | - | chr8.4:69781207-69781226 | MS.gene84862:CDS | 55.0% | |
| GGAATCTCCGGCAACGGTTA+CGG | - | chr8.4:69781588-69781607 | MS.gene84862:CDS | 55.0% | |
| GTGGGGATATGATCGTACGG+CGG | - | chr8.4:69781971-69781990 | MS.gene84862:CDS | 55.0% | |
| GTTAATATCACCGGCGGTGG+GGG | - | chr8.4:69781208-69781227 | MS.gene84862:CDS | 55.0% | |
| TTCTCGGCGCCACGGTTTAT+TGG | - | chr8.4:69781032-69781051 | MS.gene84862:CDS | 55.0% | |
| ! | GCGGATAGAGGATGATGACG+TGG | - | chr8.4:69781990-69782009 | MS.gene84862:CDS | 55.0% |
| ! | GTGGAGGAGATTTTCCGGCA+GGG | - | chr8.4:69781832-69781851 | MS.gene84862:CDS | 55.0% |
| ! | TTTTCTCCGACCTGGTCGTG+TGG | - | chr8.4:69781714-69781733 | MS.gene84862:CDS | 55.0% |
| !! | ACACAAGCTTTTTCCGGCGG+TGG | - | chr8.4:69781813-69781832 | MS.gene84862:CDS | 55.0% |
| !! | CGGTGATGGAAAAGGGTGTG+GGG | - | chr8.4:69781954-69781973 | MS.gene84862:CDS | 55.0% |
| !! | GACGGTGATGGAAAAGGGTG+TGG | - | chr8.4:69781952-69781971 | MS.gene84862:CDS | 55.0% |
| AGACAGGTTTCTCGGCGCCA+CGG | - | chr8.4:69781024-69781043 | MS.gene84862:CDS | 60.0% | |
| ATCTCCGGCAACGGTTACGG+CGG | - | chr8.4:69781591-69781610 | MS.gene84862:CDS | 60.0% | |
| CGAATCGGAACTCGCCGAGT+CGG | - | chr8.4:69781896-69781915 | MS.gene84862:CDS | 60.0% | |
| CGGAAAATCTCCTCCACCGC+CGG | + | chr8.4:69781829-69781848 | None:intergenic | 60.0% | |
| GAACGTCCACACGACCAGGT+CGG | + | chr8.4:69781723-69781742 | None:intergenic | 60.0% | |
| GAATCGGAACTCGCCGAGTC+GGG | - | chr8.4:69781897-69781916 | MS.gene84862:CDS | 60.0% | |
| GACTCAAACTCGCTCCCTGC+CGG | + | chr8.4:69781849-69781868 | None:intergenic | 60.0% | |
| GCAGGGAGCGAGTTTGAGTC+CGG | - | chr8.4:69781849-69781868 | MS.gene84862:CDS | 60.0% | |
| ! | CGGCGGTGGAGGAGATTTTC+CGG | - | chr8.4:69781827-69781846 | MS.gene84862:CDS | 60.0% |
| ! | GGTGGAGGAGATTTTCCGGC+AGG | - | chr8.4:69781831-69781850 | MS.gene84862:CDS | 60.0% |
| !! | CAAGCTTTTTCCGGCGGTGG+AGG | - | chr8.4:69781816-69781835 | MS.gene84862:CDS | 60.0% |
| AATATCACCGGCGGTGGGGG+TGG | - | chr8.4:69781211-69781230 | MS.gene84862:CDS | 65.0% | |
| GGGAGCGAGTTTGAGTCCGG+CGG | - | chr8.4:69781852-69781871 | MS.gene84862:CDS | 65.0% | |
| TCCGGCAACGGTTACGGCGG+CGG | - | chr8.4:69781594-69781613 | MS.gene84862:CDS | 70.0% | |
| !! | GGGGTGGTGGAAGGAGGTGG+AGG | - | chr8.4:69781227-69781246 | MS.gene84862:CDS | 70.0% |
| !! | GTGGGGGTGGTGGAAGGAGG+TGG | - | chr8.4:69781224-69781243 | MS.gene84862:CDS | 70.0% |
| ATCACCGGCGGTGGGGGTGG+TGG | - | chr8.4:69781214-69781233 | MS.gene84862:CDS | 75.0% | |
| CCCGCCGCCGTAACCGTTGC+CGG | + | chr8.4:69781598-69781617 | None:intergenic | 75.0% | |
| CCGGCAACGGTTACGGCGGC+GGG | - | chr8.4:69781595-69781614 | MS.gene84862:CDS | 75.0% | |
| CCTTCCACCACCCCCACCGC+CGG | + | chr8.4:69781221-69781240 | None:intergenic | 75.0% | |
| CGGCAACGGTTACGGCGGCG+GGG | - | chr8.4:69781596-69781615 | MS.gene84862:CDS | 75.0% | |
| !! | GCGGTGGGGGTGGTGGAAGG+AGG | - | chr8.4:69781221-69781240 | MS.gene84862:CDS | 75.0% |
| !!! | CCGGCGGTGGGGGTGGTGGA+AGG | - | chr8.4:69781218-69781237 | MS.gene84862:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 69780881 | 69782152 | 69780881 | ID=MS.gene84862 |
| chr8.4 | mRNA | 69780881 | 69782152 | 69780881 | ID=MS.gene84862.t1;Parent=MS.gene84862 |
| chr8.4 | exon | 69780881 | 69782152 | 69780881 | ID=MS.gene84862.t1.exon1;Parent=MS.gene84862.t1 |
| chr8.4 | CDS | 69780881 | 69782152 | 69780881 | ID=cds.MS.gene84862.t1;Parent=MS.gene84862.t1 |
| Gene Sequence |
| Protein sequence |
