AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84869

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84869.t1 MTR_1g064240 96.949 295 9 0 1 295 416 710 0.0 599
MS.gene84869.t1 MTR_2g094860 62.799 293 98 2 1 293 422 703 4.09e-131 388
MS.gene84869.t1 MTR_4g058730 74.766 214 54 0 1 214 404 617 1.51e-114 343
MS.gene84869.t1 MTR_2g460590 58.000 300 115 3 1 295 396 689 1.31e-106 325
MS.gene84869.t1 MTR_1g028370 55.932 295 120 3 1 293 428 714 5.96e-105 321
MS.gene84869.t1 MTR_3g109030 55.369 298 123 2 1 291 426 720 1.72e-104 320
MS.gene84869.t1 MTR_7g034605 54.882 297 118 3 1 293 396 680 4.85e-102 313
MS.gene84869.t1 MTR_3g091080 54.237 295 124 4 1 293 426 711 1.79e-101 312
MS.gene84869.t1 MTR_4g130820 55.405 296 119 5 1 295 448 731 8.84e-101 311
MS.gene84869.t1 MTR_3g088875 52.824 301 131 3 1 291 388 687 1.87e-99 306
MS.gene84869.t1 MTR_7g117310 60.268 224 88 1 1 223 377 600 5.44e-90 281
MS.gene84869.t1 MTR_7g117310 60.268 224 88 1 1 223 431 654 8.85e-90 282
MS.gene84869.t1 MTR_7g117310 60.268 224 88 1 1 223 443 666 1.44e-89 281
MS.gene84869.t1 MTR_8g027755 57.399 223 94 1 1 222 435 657 6.60e-85 269
MS.gene84869.t1 MTR_8g464570 41.200 250 144 2 1 249 417 664 5.00e-51 178
MS.gene84869.t1 MTR_2g081110 44.811 212 114 2 1 211 413 622 4.06e-50 176
MS.gene84869.t1 MTR_7g012260 41.509 212 121 2 1 211 465 674 1.14e-44 161
MS.gene84869.t1 MTR_6g477760 39.301 229 118 7 1 216 475 695 1.41e-37 141
MS.gene84869.t1 MTR_5g007630 38.750 240 126 6 1 226 509 741 2.11e-37 140
MS.gene84869.t1 MTR_6g075440 37.872 235 125 8 1 221 514 741 4.10e-36 137
MS.gene84869.t1 MTR_6g075440 37.872 235 125 8 1 221 514 741 4.20e-36 137
MS.gene84869.t1 MTR_6g075290 40.088 227 111 6 1 212 499 715 1.75e-35 135
MS.gene84869.t1 MTR_6g075290 40.088 227 111 6 1 212 338 554 2.71e-35 134
MS.gene84869.t1 MTR_6g075290 40.088 227 111 6 1 212 540 756 3.22e-35 134
MS.gene84869.t1 MTR_6g076060 38.839 224 117 5 1 211 242 458 4.79e-35 132
MS.gene84869.t1 MTR_6g075390 39.037 187 95 4 1 175 457 636 2.73e-29 117
MS.gene84869.t1 MTR_6g075410 33.476 233 131 8 1 219 443 665 8.49e-24 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84869.t1 AT2G28260 60.068 293 90 1 1 293 373 638 6.15e-118 352
MS.gene84869.t1 AT2G28260 60.068 293 90 1 1 293 413 678 1.03e-117 353
MS.gene84869.t1 AT1G15990 54.027 298 129 2 1 293 444 738 2.32e-105 323
MS.gene84869.t1 AT1G19780 55.068 296 126 2 1 290 239 533 3.60e-105 317
MS.gene84869.t1 AT4G30360 54.698 298 131 2 1 295 423 719 4.67e-105 322
MS.gene84869.t1 AT1G19780 55.068 296 126 2 1 290 450 744 1.01e-102 317
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 429 718 3.46e-102 314
MS.gene84869.t1 AT5G57940 54.545 297 112 2 1 293 433 710 7.66e-102 313
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 456 745 7.79e-102 314
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 456 745 7.79e-102 314
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 456 745 7.79e-102 314
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 456 745 7.79e-102 314
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 456 745 7.79e-102 314
MS.gene84869.t1 AT5G57940 54.545 297 112 2 1 293 440 717 9.92e-102 313
MS.gene84869.t1 AT5G57940 54.545 297 112 2 1 293 440 717 9.92e-102 313
MS.gene84869.t1 AT2G24610 53.595 306 126 2 1 293 423 725 1.85e-101 312
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 479 768 2.12e-101 313
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 479 768 2.12e-101 313
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 479 768 2.12e-101 313
MS.gene84869.t1 AT2G23980 53.584 293 133 1 1 293 479 768 2.12e-101 313
MS.gene84869.t1 AT2G24610 53.595 306 126 2 1 293 438 740 4.69e-101 312
MS.gene84869.t1 AT4G30560 53.741 294 117 2 1 293 429 704 1.29e-100 310
MS.gene84869.t1 AT4G30560 53.741 294 117 2 1 293 429 704 1.29e-100 310
MS.gene84869.t1 AT4G30560 53.741 294 117 2 1 293 429 704 1.29e-100 310
MS.gene84869.t1 AT4G30560 53.741 294 117 2 1 293 455 730 1.62e-100 310
MS.gene84869.t1 AT4G30560 53.741 294 117 2 1 293 455 730 1.62e-100 310
MS.gene84869.t1 AT1G01340 51.351 296 137 3 1 293 410 701 2.80e-96 298
MS.gene84869.t1 AT1G01340 51.351 296 137 3 1 293 415 706 2.88e-96 298
MS.gene84869.t1 AT5G14870 50.313 320 128 5 1 295 391 704 3.15e-96 298
MS.gene84869.t1 AT5G53130 53.584 293 131 2 1 293 428 715 6.17e-96 298
MS.gene84869.t1 AT3G48010 63.348 221 81 0 1 221 399 619 1.84e-92 288
MS.gene84869.t1 AT4G01010 52.055 292 124 5 1 291 416 692 1.24e-91 286
MS.gene84869.t1 AT4G01010 52.055 292 124 5 1 291 416 692 1.24e-91 286
MS.gene84869.t1 AT2G46430 49.320 294 140 5 1 293 419 704 4.07e-84 267
MS.gene84869.t1 AT2G46430 49.320 294 140 5 1 293 419 704 4.07e-84 267
MS.gene84869.t1 AT2G46440 51.681 238 109 3 1 238 199 430 4.28e-68 218
MS.gene84869.t1 AT2G46440 51.681 238 109 3 1 238 377 608 2.25e-66 218
MS.gene84869.t1 AT2G46450 40.889 225 124 3 1 224 341 557 1.99e-51 178
MS.gene84869.t1 AT2G46450 40.889 225 124 3 1 224 354 570 2.35e-51 178
MS.gene84869.t1 AT2G46450 40.889 225 124 3 1 224 377 593 4.09e-51 178
MS.gene84869.t1 AT2G46450 40.889 225 124 3 1 224 377 593 4.09e-51 178
MS.gene84869.t1 AT5G54250 40.400 250 146 2 1 249 438 685 5.18e-50 176
MS.gene84869.t1 AT5G54250 40.400 250 146 2 1 249 438 685 5.18e-50 176
MS.gene84869.t1 AT5G54250 40.400 250 146 2 1 249 438 685 5.18e-50 176
MS.gene84869.t1 AT5G54250 38.800 250 145 3 1 249 438 680 1.26e-44 161
MS.gene84869.t1 AT5G15410 42.029 207 117 2 1 206 472 676 4.25e-43 157
MS.gene84869.t1 AT5G15410 42.029 207 117 2 1 206 473 677 4.49e-43 157
MS.gene84869.t1 AT3G17700 39.631 217 113 6 1 206 536 745 1.35e-36 139
MS.gene84869.t1 AT3G17690 36.481 233 131 7 1 223 503 728 1.50e-36 138
MS.gene84869.t1 AT3G17700 41.304 138 74 2 1 138 536 666 1.64e-26 109
MS.gene84869.t1 AT3G17690 39.130 138 77 2 1 138 503 633 7.83e-25 104

Find 62 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGTTATTCAATGTATTC+TGG 0.255289 6.4:-31958405 MS.gene84869:CDS
TTAATGATGATGGTGATTAA+AGG 0.259764 6.4:-31958287 None:intergenic
ATGTCAAATGTGAAATATTA+TGG 0.260963 6.4:-31958477 MS.gene84869:CDS
TTGATATGCTTGGTACATTA+AGG 0.286363 6.4:-31958329 MS.gene84869:CDS
ATTGAATAACTTGATCCTTT+TGG 0.295632 6.4:+31958414 None:intergenic
TCATTAAAATCTGGTTCAAT+AGG 0.330248 6.4:+31958303 None:intergenic
GACAAATGAGGTTAAGGAAA+AGG 0.372638 6.4:-31958514 MS.gene84869:CDS
AGCGGTCGGTCGAGGGTCTA+AGG 0.374021 6.4:+31958749 None:intergenic
GAAAACTAGTAGGTAGTCTA+AGG 0.374905 6.4:-31958383 MS.gene84869:CDS
TTATACAAGCAGATTGGTTT+CGG 0.378087 6.4:-31958554 MS.gene84869:CDS
GAAAGAGACAAATGAGGTTA+AGG 0.381363 6.4:-31958520 MS.gene84869:CDS
TCACCATCATCATTAAAATC+TGG 0.392568 6.4:+31958294 None:intergenic
GACGCCATGACCAATTTAGA+TGG 0.405101 6.4:-31959534 MS.gene84869:CDS
TCGCAAACTATGCAAGTTCC+TGG 0.407909 6.4:+31958912 None:intergenic
GGAAGCACAGCGGTCGGTCG+AGG 0.409057 6.4:+31958741 None:intergenic
ATGTCACGTCTTAGATCAAC+AGG 0.421675 6.4:+31959466 None:intergenic
TCAGTTGTTTGCTATGTAAC+CGG 0.425623 6.4:+31958628 None:intergenic
AGAGGAAGAAGCATGAGATA+TGG 0.449560 6.4:-31958360 MS.gene84869:CDS
ATATTTCTCTGCACCTCTGC+AGG 0.451022 6.4:-31958439 MS.gene84869:CDS
ACGACTAACGGTGGTAGAAC+TGG 0.452762 6.4:-31958834 MS.gene84869:CDS
GAGTAGCTACCCATCTAAAT+TGG 0.455345 6.4:+31959524 None:intergenic
CTCACTGTGCGAGTTGATGA+AGG 0.471556 6.4:+31958720 None:intergenic
ATGTATTCTGGAAAACTAGT+AGG 0.473124 6.4:-31958393 MS.gene84869:CDS
GTCGCCTGAGCCTATTCTGC+AGG 0.482833 6.4:+31958800 None:intergenic
TTACTTGTCTAACTAGATCA+AGG 0.483343 6.4:+31959428 None:intergenic
ATGAAGAGCATTTCGTCGAC+AGG 0.493141 6.4:+31958882 None:intergenic
CAGAAAGATGGATGCATCAC+AGG 0.496553 6.4:-31959588 None:intergenic
TGCTACAAACTTTAAATCCT+CGG 0.498284 6.4:+31958662 None:intergenic
GCACCTCTGCAGGTGCCAAA+AGG 0.504350 6.4:-31958429 MS.gene84869:CDS
GAAATGCTCTTCATCGTTCG+AGG 0.508603 6.4:-31958873 MS.gene84869:CDS
TTAGATTCTTGCACGACTAA+CGG 0.521241 6.4:-31958846 MS.gene84869:CDS
TCGTCCATCTGATCAAATAG+TGG 0.528135 6.4:+31958984 None:intergenic
GTTCCTGGAGTGCATAGAGT+TGG 0.542992 6.4:+31958927 None:intergenic
GGTTCTGAACTTGATATGCT+TGG 0.544592 6.4:-31958339 MS.gene84869:CDS
AACACCTGCAGAATAGGCTC+AGG 0.545167 6.4:-31958804 MS.gene84869:CDS
ACTCACATCAGTGGAGGACA+TGG 0.553664 6.4:-31958587 MS.gene84869:CDS
GAGAATGAAAGAGACAAATG+AGG 0.556459 6.4:-31958526 MS.gene84869:CDS
TTGTAGCAGCACAATTCTGC+CGG 0.557395 6.4:-31958647 MS.gene84869:CDS
GGTACCACTATTTGATCAGA+TGG 0.566255 6.4:-31958988 MS.gene84869:CDS
AAAAGCTTCGACTTCTGTGA+TGG 0.577464 6.4:+31958695 None:intergenic
CTACCCATCTAAATTGGTCA+TGG 0.585160 6.4:+31959530 None:intergenic
GAAGCACAGCGGTCGGTCGA+GGG 0.585728 6.4:+31958742 None:intergenic
ACGCCATGACCAATTTAGAT+GGG 0.585828 6.4:-31959533 MS.gene84869:CDS
TATTTGATCAGATGGACGAC+AGG 0.586329 6.4:-31958980 MS.gene84869:CDS
TGTTAGATGCAATACGTGAA+AGG 0.587308 6.4:-31958956 MS.gene84869:CDS
AAACCAACTCTATGCACTCC+AGG 0.588925 6.4:-31958930 MS.gene84869:CDS
AGCTACTCGTGGAGTTGACG+AGG 0.602552 6.4:-31959509 MS.gene84869:CDS
CTGATGTGAGTAGAATCTAA+AGG 0.605058 6.4:+31958599 None:intergenic
GGTCGAGGGTCTAAGGCCCA+TGG 0.605184 6.4:+31958756 None:intergenic
GGAACTTGCATAGTTTGCGA+AGG 0.609337 6.4:-31958909 MS.gene84869:CDS
CTAGTAGGTAGTCTAAGGAG+AGG 0.620088 6.4:-31958378 MS.gene84869:CDS
GATGAAGGAAGCACAGCGGT+CGG 0.622836 6.4:+31958735 None:intergenic
GCAGCTCCCTCGTTACCTGA+AGG 0.626636 6.4:-31959566 MS.gene84869:CDS
TTTAGATGGGTAGCTACTCG+TGG 0.627869 6.4:-31959520 MS.gene84869:CDS
TTAGATTCTACTCACATCAG+TGG 0.629474 6.4:-31958596 MS.gene84869:CDS
ATAGGCTCAGGCGACTTCTG+CGG 0.636175 6.4:-31958792 MS.gene84869:CDS
GATTCTACTCACATCAGTGG+AGG 0.638528 6.4:-31958593 MS.gene84869:CDS
TGAAGAGCATTTCGTCGACA+GGG 0.649007 6.4:+31958883 None:intergenic
CTCACATCAGTGGAGGACAT+GGG 0.670776 6.4:-31958586 MS.gene84869:CDS
TAACTAGATCAAGGCAAAAG+CGG 0.683572 6.4:+31959437 None:intergenic
GATTCTTGCACGACTAACGG+TGG 0.726903 6.4:-31958843 MS.gene84869:CDS
AGTTGATGAAGGAAGCACAG+CGG 0.802077 6.4:+31958731 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTATGTGATTAAGAG+TGG + chr6.4:31958750-31958769 None:intergenic 20.0%
!! ATGTCAAATGTGAAATATTA+TGG - chr6.4:31959390-31959409 MS.gene84869:intron 20.0%
!! ATTCACTAATTTAGATTAGA+CGG - chr6.4:31958540-31958559 MS.gene84869:CDS 20.0%
!! TTCTAAATCAGTAACAATTA+TGG - chr6.4:31958615-31958634 MS.gene84869:CDS 20.0%
!!! AATAAGAATGAAGTACTAAT+TGG - chr6.4:31958842-31958861 MS.gene84869:CDS 20.0%
! ATATTCCAGAAATTCATTCA+TGG - chr6.4:31958650-31958669 MS.gene84869:CDS 25.0%
! TCAAGTTATTCAATGTATTC+TGG - chr6.4:31959462-31959481 MS.gene84869:CDS 25.0%
!! ATTGAATAACTTGATCCTTT+TGG + chr6.4:31959456-31959475 None:intergenic 25.0%
!! GCTTTTTATTACACAGATTA+TGG - chr6.4:31958496-31958515 MS.gene84869:CDS 25.0%
!! TCATTAAAATCTGGTTCAAT+AGG + chr6.4:31959567-31959586 None:intergenic 25.0%
TCACCATCATCATTAAAATC+TGG + chr6.4:31959576-31959595 None:intergenic 30.0%
TGCTACAAACTTTAAATCCT+CGG + chr6.4:31959208-31959227 None:intergenic 30.0%
TTACTTGTCTAACTAGATCA+AGG + chr6.4:31958442-31958461 None:intergenic 30.0%
TTGATATGCTTGGTACATTA+AGG - chr6.4:31959538-31959557 MS.gene84869:CDS 30.0%
TTTGACCATGAATGAATTTC+TGG + chr6.4:31958658-31958677 None:intergenic 30.0%
! ATGTATTCTGGAAAACTAGT+AGG - chr6.4:31959474-31959493 MS.gene84869:CDS 30.0%
! CATGTTTTATACAAGCAGAT+TGG - chr6.4:31959307-31959326 MS.gene84869:intron 30.0%
!! GAACCAGATTTTAATGATGA+TGG - chr6.4:31959570-31959589 MS.gene84869:CDS 30.0%
!! TTATACAAGCAGATTGGTTT+CGG - chr6.4:31959313-31959332 MS.gene84869:intron 30.0%
CTGATGTGAGTAGAATCTAA+AGG + chr6.4:31959271-31959290 None:intergenic 35.0%
GAAAACTAGTAGGTAGTCTA+AGG - chr6.4:31959484-31959503 MS.gene84869:CDS 35.0%
GAAAGAGACAAATGAGGTTA+AGG - chr6.4:31959347-31959366 MS.gene84869:intron 35.0%
GACAAATGAGGTTAAGGAAA+AGG - chr6.4:31959353-31959372 MS.gene84869:intron 35.0%
GAGAATGAAAGAGACAAATG+AGG - chr6.4:31959341-31959360 MS.gene84869:intron 35.0%
TAACTAGATCAAGGCAAAAG+CGG + chr6.4:31958433-31958452 None:intergenic 35.0%
TAATTGGACACTAATTGCAC+AGG - chr6.4:31958858-31958877 MS.gene84869:CDS 35.0%
TATTTGCACGTAAAGTAGAG+CGG + chr6.4:31958585-31958604 None:intergenic 35.0%
TGTTAGATGCAATACGTGAA+AGG - chr6.4:31958911-31958930 MS.gene84869:CDS 35.0%
TTAGATTCTACTCACATCAG+TGG - chr6.4:31959271-31959290 MS.gene84869:intron 35.0%
TTAGATTCTTGCACGACTAA+CGG - chr6.4:31959021-31959040 MS.gene84869:intron 35.0%
!! AAAAAAAAGTGTCGCACACA+TGG + chr6.4:31958782-31958801 None:intergenic 35.0%
!! TCAGTTGTTTGCTATGTAAC+CGG + chr6.4:31959242-31959261 None:intergenic 35.0%
!! TTTTTCAACACCTGCAGAAT+AGG - chr6.4:31959057-31959076 MS.gene84869:intron 35.0%
AAAAGCTTCGACTTCTGTGA+TGG + chr6.4:31959175-31959194 None:intergenic 40.0%
ACGCCATGACCAATTTAGAT+GGG - chr6.4:31958334-31958353 MS.gene84869:CDS 40.0%
ATGTCACGTCTTAGATCAAC+AGG + chr6.4:31958404-31958423 None:intergenic 40.0%
CTACCCATCTAAATTGGTCA+TGG + chr6.4:31958340-31958359 None:intergenic 40.0%
GACCAATGCGATCTCAATTT+CGG - chr6.4:31958681-31958700 MS.gene84869:CDS 40.0%
GAGTAGCTACCCATCTAAAT+TGG + chr6.4:31958346-31958365 None:intergenic 40.0%
GGTACCACTATTTGATCAGA+TGG - chr6.4:31958879-31958898 MS.gene84869:CDS 40.0%
TATTTGATCAGATGGACGAC+AGG - chr6.4:31958887-31958906 MS.gene84869:CDS 40.0%
TCGTCCATCTGATCAAATAG+TGG + chr6.4:31958886-31958905 None:intergenic 40.0%
! ACATTTTCCTTCAGGTAACG+AGG + chr6.4:31958311-31958330 None:intergenic 40.0%
! AGAGGAAGAAGCATGAGATA+TGG - chr6.4:31959507-31959526 MS.gene84869:CDS 40.0%
! CATTTTCCTTCAGGTAACGA+GGG + chr6.4:31958310-31958329 None:intergenic 40.0%
!! GGTTCTGAACTTGATATGCT+TGG - chr6.4:31959528-31959547 MS.gene84869:CDS 40.0%
AAACCAACTCTATGCACTCC+AGG - chr6.4:31958937-31958956 MS.gene84869:CDS 45.0%
ATATTTCTCTGCACCTCTGC+AGG - chr6.4:31959428-31959447 MS.gene84869:intron 45.0%
CTAGTAGGTAGTCTAAGGAG+AGG - chr6.4:31959489-31959508 MS.gene84869:CDS 45.0%
GAAATGCTCTTCATCGTTCG+AGG - chr6.4:31958994-31959013 MS.gene84869:intron 45.0%
GACCGAAATTGAGATCGCAT+TGG + chr6.4:31958686-31958705 None:intergenic 45.0%
GACGCCATGACCAATTTAGA+TGG - chr6.4:31958333-31958352 MS.gene84869:CDS 45.0%
GATTCTACTCACATCAGTGG+AGG - chr6.4:31959274-31959293 MS.gene84869:intron 45.0%
TCGCAAACTATGCAAGTTCC+TGG + chr6.4:31958958-31958977 None:intergenic 45.0%
TTGTAGCAGCACAATTCTGC+CGG - chr6.4:31959220-31959239 MS.gene84869:intron 45.0%
TTTAGATGGGTAGCTACTCG+TGG - chr6.4:31958347-31958366 MS.gene84869:CDS 45.0%
! CGGTGAAGAGCTTTTACCAT+GGG - chr6.4:31959095-31959114 MS.gene84869:intron 45.0%
! GGAACTTGCATAGTTTGCGA+AGG - chr6.4:31958958-31958977 MS.gene84869:CDS 45.0%
!! AGTTGATGAAGGAAGCACAG+CGG + chr6.4:31959139-31959158 None:intergenic 45.0%
!! ATGAAGAGCATTTCGTCGAC+AGG + chr6.4:31958988-31959007 None:intergenic 45.0%
!! TGAAGAGCATTTCGTCGACA+GGG + chr6.4:31958987-31959006 None:intergenic 45.0%
AACACCTGCAGAATAGGCTC+AGG - chr6.4:31959063-31959082 MS.gene84869:intron 50.0%
ACGACTAACGGTGGTAGAAC+TGG - chr6.4:31959033-31959052 MS.gene84869:intron 50.0%
ACTCACATCAGTGGAGGACA+TGG - chr6.4:31959280-31959299 MS.gene84869:intron 50.0%
CTCACATCAGTGGAGGACAT+GGG - chr6.4:31959281-31959300 MS.gene84869:intron 50.0%
GATTCTTGCACGACTAACGG+TGG - chr6.4:31959024-31959043 MS.gene84869:intron 50.0%
GTTCCTGGAGTGCATAGAGT+TGG + chr6.4:31958943-31958962 None:intergenic 50.0%
! AGCTTTTGCACTCATTGCCG+AGG - chr6.4:31959188-31959207 MS.gene84869:intron 50.0%
! GCGGTGAAGAGCTTTTACCA+TGG - chr6.4:31959094-31959113 MS.gene84869:intron 50.0%
!! CTCACTGTGCGAGTTGATGA+AGG + chr6.4:31959150-31959169 None:intergenic 50.0%
!! GGCGTCGAACATTTTCCTTC+AGG + chr6.4:31958319-31958338 None:intergenic 50.0%
AGCTACTCGTGGAGTTGACG+AGG - chr6.4:31958358-31958377 MS.gene84869:CDS 55.0%
ATAGGCTCAGGCGACTTCTG+CGG - chr6.4:31959075-31959094 MS.gene84869:intron 55.0%
! GATCCTTTTGGCACCTGCAG+AGG + chr6.4:31959444-31959463 None:intergenic 55.0%
! GATGAAGGAAGCACAGCGGT+CGG + chr6.4:31959135-31959154 None:intergenic 55.0%
GCACCTCTGCAGGTGCCAAA+AGG - chr6.4:31959438-31959457 MS.gene84869:CDS 60.0%
GCAGCTCCCTCGTTACCTGA+AGG - chr6.4:31958301-31958320 MS.gene84869:CDS 60.0%
GTCGCCTGAGCCTATTCTGC+AGG + chr6.4:31959070-31959089 None:intergenic 60.0%
AGCGGTCGGTCGAGGGTCTA+AGG + chr6.4:31959121-31959140 None:intergenic 65.0%
GAAGCACAGCGGTCGGTCGA+GGG + chr6.4:31959128-31959147 None:intergenic 65.0%
! GGTCGAGGGTCTAAGGCCCA+TGG + chr6.4:31959114-31959133 None:intergenic 65.0%
! GGAAGCACAGCGGTCGGTCG+AGG + chr6.4:31959129-31959148 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 31958290 31959599 31958290 ID=MS.gene84869
chr6.4 mRNA 31958290 31959599 31958290 ID=MS.gene84869.t1;Parent=MS.gene84869
chr6.4 exon 31959432 31959599 31959432 ID=MS.gene84869.t1.exon1;Parent=MS.gene84869.t1
chr6.4 CDS 31959432 31959599 31959432 ID=cds.MS.gene84869.t1;Parent=MS.gene84869.t1
chr6.4 exon 31958290 31959009 31958290 ID=MS.gene84869.t1.exon2;Parent=MS.gene84869.t1
chr6.4 CDS 31958290 31959009 31958290 ID=cds.MS.gene84869.t1;Parent=MS.gene84869.t1
Gene Sequence

>MS.gene84869

ATGCATCACAGGCAGCTCCCTCGTTACCTGAAGGAAAATGTTCGACGCCATGACCAATTTAGATGGGTAGCTACTCGTGGAGTTGACGAGGAAGCAATTCTTAGAGATCTTCCTGTTGATCTAAGACGTGACATTAAACGCCGCTTTTGCCTTGATCTAGTTAGACAAGTAAGTAAATCTTTGTTTTCATCTCTGCATCTGTATTTGCTTTTTATTACACAGATTATGGATTAAACTACTTAGATTACACATTCACTAATTTAGATTAGACGGATCTGCTGAGAATCTGTTTCCGCTCTACTTTACGTGCAAATAAGATTTTAACTTCTAAATCAGTAACAATTATGGTCTCAATCACTGATATTCCAGAAATTCATTCATGGTCAAATTTGACCAATGCGATCTCAATTTCGGTCGCATAAACGTGATCATTGATAAATAAAAAATCTTGATGTCTCCACTCTTAATCACATAATTTTTCTTAAGAAACCATGTGTGCGACACTTTTTTTTCTTTTCTTGTTGATATGTTTCTTGAGAAACAAGCAAACAAAATAAGAATGAAGTACTAATTGGACACTAATTGCACAGGTACCACTATTTGATCAGATGGACGACAGGATGTTAGATGCAATACGTGAAAGGCTAAAACCAACTCTATGCACTCCAGGAACTTGCATAGTTTGCGAAGGAGACCCTGTCGACGAAATGCTCTTCATCGTTCGAGGACGTTTAGATTCTTGCACGACTAACGGTGGTAGAACTGGTTTTTTCAACACCTGCAGAATAGGCTCAGGCGACTTCTGCGGTGAAGAGCTTTTACCATGGGCCTTAGACCCTCGACCGACCGCTGTGCTTCCTTCATCAACTCGCACAGTGAGAGCCATCACAGAAGTCGAAGCTTTTGCACTCATTGCCGAGGATTTAAAGTTTGTAGCAGCACAATTCTGCCGGTTACATAGCAAACAACTGAGACAAACCTTTAGATTCTACTCACATCAGTGGAGGACATGGGTTGCATGTTTTATACAAGCAGATTGGTTTCGGTATAAGAGAATGAAAGAGACAAATGAGGTTAAGGAAAAGGAGAATCTTATGATGATGTCAAATGTGAAATATTATGGAAATGATGATTCTCAATATTTCTCTGCACCTCTGCAGGTGCCAAAAGGATCAAGTTATTCAATGTATTCTGGAAAACTAGTAGGTAGTCTAAGGAGAGGAAGAAGCATGAGATATGGTTCTGAACTTGATATGCTTGGTACATTAAGGAAGCCTATTGAACCAGATTTTAATGATGATGGTGATTAA
Protein sequence

>MS.gene84869.t1

MHHRQLPRYLKENVRRHDQFRWVATRGVDEEAILRDLPVDLRRDIKRRFCLDLVRQVPLFDQMDDRMLDAIRERLKPTLCTPGTCIVCEGDPVDEMLFIVRGRLDSCTTNGGRTGFFNTCRIGSGDFCGEELLPWALDPRPTAVLPSSTRTVRAITEVEAFALIAEDLKFVAAQFCRLHSKQLRQTFRFYSHQWRTWVACFIQADWFRYKRMKETNEVKEKENLMMMSNVKYYGNDDSQYFSAPLQVPKGSSYSMYSGKLVGSLRRGRSMRYGSELDMLGTLRKPIEPDFNDDGD