AlfalfaGEDB | MS.gene85185

M. sativa cultivar XinJiangDaYe / MS.gene85185

KEGG: K12197
GO: GO:0006810 GO:0007034 GO:0008150 GO:0051179 GO:0051234
PFAM: PF03357.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85185.t1	XP_003597636.1	100	205	0	0	1	205	1	205	2.90E-99	371.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85185.t1	Q8LE58	87.8	205	23	1	1	205	1	203	2.5e-88	326.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85185.t1	B7FHV8	100.0	205	0	0	1	205	1	205	2.1e-99	371.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene76575	MS.gene85185	PPI
MS.gene85185	MS.gene36391	PPI
MS.gene85185	MS.gene069907	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85185.t1	MTR_2g100420	100.000	205	0	0	1	205	1	205	2.80e-150	415

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85185.t1	AT1G17730	87.805	205	23	1	1	205	1	203	1.04e-130	366
MS.gene85185.t1	AT1G73030	88.293	205	22	1	1	205	1	203	8.49e-119	335
MS.gene85185.t1	AT2G06530	27.273	165	116	2	19	180	7	170	6.49e-12	62.4
MS.gene85185.t1	AT2G06530	27.273	165	116	2	19	180	28	191	8.56e-12	62.4

Find 49 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGGTTCAAGCGGAGTTTA+TGG	0.198154	2.4:+3986204	MS.gene85185:CDS
CAACAGGTGGCGGATGATTA+TGG	0.251058	2.4:+3986289	MS.gene85185:CDS
CGAGAAGCAGTTTGTGAATA+TGG	0.254306	2.4:+3986183	MS.gene85185:CDS
GGGAAACATCGTGAAATCTT+TGG	0.350209	2.4:+3986105	MS.gene85185:CDS
GGCGGCTGCTCATGCTGTTC+CGG	0.374454	2.4:+3986339	MS.gene85185:CDS
TGCTCATGCTGTTCCGGTTA+AGG	0.374890	2.4:+3986345	MS.gene85185:CDS
GGAAGTTTCGGTGAATCTTC+CGG	0.375555	2.4:+3986315	MS.gene85185:CDS
TCAGCATCCTTCTCCTTAAC+CGG	0.398938	2.4:-3986358	None:intergenic
CATCCAATCGGGAGGATAAT+CGG	0.409666	2.4:-3986027	None:intergenic
TGATTATGGATTGGAAGTTT+CGG	0.427917	2.4:+3986303	MS.gene85185:CDS
GGTTAATAGTTTGATGCAAC+AGG	0.440152	2.4:+3986273	MS.gene85185:CDS
TTGGAATCGTCGTTGGCGAC+GGG	0.441222	2.4:+3986124	MS.gene85185:CDS
TTCGGTGAATCTTCCGGTGG+CGG	0.464892	2.4:+3986321	MS.gene85185:CDS
AAGCTTCTCTGTGTTTCCCA+TGG	0.476337	2.4:-3985819	None:intergenic
TAGTTTGATGCAACAGGTGG+CGG	0.477361	2.4:+3986279	MS.gene85185:CDS
AAACTATTAACCTCCCCTTC+AGG	0.482512	2.4:-3986262	None:intergenic
GAGCACTCACGGCATCCAAT+CGG	0.483420	2.4:-3986039	None:intergenic
ACAGCATGAGCAGCCGCCAC+CGG	0.500440	2.4:-3986334	None:intergenic
AGCACTCACGGCATCCAATC+GGG	0.502128	2.4:-3986038	None:intergenic
CTTCGCTTTCGACTCCTGAA+GGG	0.513104	2.4:+3986248	MS.gene85185:CDS
ACTTCGCTTTCGACTCCTGA+AGG	0.521847	2.4:+3986247	MS.gene85185:CDS
GTCAACAATCAGCAAATCGA+TGG	0.522743	2.4:+3986084	MS.gene85185:CDS
TGCTGTTCCGGTTAAGGAGA+AGG	0.523122	2.4:+3986351	MS.gene85185:CDS
GAAGCTTCTGAATCAGATCA+TGG	0.534967	2.4:+3985835	MS.gene85185:CDS
GAAATCTTTGGAATCGTCGT+TGG	0.542053	2.4:+3986117	MS.gene85185:CDS
TTTGGAATCGTCGTTGGCGA+CGG	0.545316	2.4:+3986123	MS.gene85185:CDS
TTCGCTTTCGACTCCTGAAG+GGG	0.555513	2.4:+3986249	MS.gene85185:CDS
GCAGAAGATGTCGGAGACGA+TGG	0.561045	2.4:+3986153	MS.gene85185:CDS
TAAGGAGAAGGATGCTGAGA+AGG	0.573843	2.4:+3986363	MS.gene85185:CDS
GGAGTTTATGGAAAGTGCTA+TGG	0.582336	2.4:+3986216	MS.gene85185:CDS
GGTGGCGGATGATTATGGAT+TGG	0.587630	2.4:+3986294	MS.gene85185:CDS
CTTGCTGAGCTTAAAGCTAG+AGG	0.589782	2.4:+3986412	MS.gene85185:CDS
CCTCCGATTATCCTCCCGAT+TGG	0.590956	2.4:+3986024	MS.gene85185:CDS
GGATGCTGAGAAGGTTGATG+AGG	0.596267	2.4:+3986372	MS.gene85185:CDS
GTTTATGGAAAGTGCTATGG+CGG	0.596862	2.4:+3986219	MS.gene85185:CDS
TGTGAATATGGAGGTTCAAG+CGG	0.607620	2.4:+3986195	MS.gene85185:CDS
TGTATCGAGACGAGCACTCA+CGG	0.607767	2.4:-3986050	None:intergenic
GCTTTCGACTCCTGAAGGGG+AGG	0.608667	2.4:+3986252	MS.gene85185:CDS
TCAACAATCAGCAAATCGAT+GGG	0.616382	2.4:+3986085	MS.gene85185:CDS
TGGAATCGTCGTTGGCGACG+GGG	0.616847	2.4:+3986125	MS.gene85185:CDS
GGGGAATTTGCAGAAGATGT+CGG	0.623637	2.4:+3986144	MS.gene85185:CDS
GAGTGCTCGTCTCGATACAC+AGG	0.631959	2.4:+3986054	MS.gene85185:CDS
TTGATGAGGATGATTTGTCA+AGG	0.632184	2.4:+3986386	MS.gene85185:CDS
TTTATGGAAAGTGCTATGGC+GGG	0.633565	2.4:+3986220	MS.gene85185:CDS
TAATAGTTTGATGCAACAGG+TGG	0.645930	2.4:+3986276	MS.gene85185:CDS
GAAGCAGTTTGTGAATATGG+AGG	0.657749	2.4:+3986186	MS.gene85185:CDS
AGTTTCGGTGAATCTTCCGG+TGG	0.669085	2.4:+3986318	MS.gene85185:CDS
ACTCACGGCATCCAATCGGG+AGG	0.700613	2.4:-3986035	None:intergenic
CCAATCGGGAGGATAATCGG+AGG	0.740590	2.4:-3986024	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTCACCTTAAGTTTTT+CGG	-	chr2.4:3985924-3985943	None:intergenic	20.0%
	AGAGAAATCCGAAAAACTTA+AGG	+	chr2.4:3985913-3985932	MS.gene85185:CDS	30.0%
	GTGAAAAAAGCAATCGAAAA+AGG	+	chr2.4:3985935-3985954	MS.gene85185:CDS	30.0%
!!	TGATTATGGATTGGAAGTTT+CGG	+	chr2.4:3986303-3986322	MS.gene85185:CDS	30.0%
	AGCAATCGAAAAAGGTAACA+TGG	+	chr2.4:3985943-3985962	MS.gene85185:CDS	35.0%
	ATCGAAAAAGGTAACATGGA+CGG	+	chr2.4:3985947-3985966	MS.gene85185:CDS	35.0%
	GGTTAATAGTTTGATGCAAC+AGG	+	chr2.4:3986273-3986292	MS.gene85185:CDS	35.0%
	TAATAGTTTGATGCAACAGG+TGG	+	chr2.4:3986276-3986295	MS.gene85185:CDS	35.0%
	TCAACAATCAGCAAATCGAT+GGG	+	chr2.4:3986085-3986104	MS.gene85185:CDS	35.0%
!	TTGATGAGGATGATTTGTCA+AGG	+	chr2.4:3986386-3986405	MS.gene85185:CDS	35.0%
!!	CTTTTTCGCATTTTCTTGCT+TGG	-	chr2.4:3985892-3985911	None:intergenic	35.0%
	AAACTATTAACCTCCCCTTC+AGG	-	chr2.4:3986265-3986284	None:intergenic	40.0%
	CGAGAAGCAGTTTGTGAATA+TGG	+	chr2.4:3986183-3986202	MS.gene85185:CDS	40.0%
	GAAGCAGTTTGTGAATATGG+AGG	+	chr2.4:3986186-3986205	MS.gene85185:CDS	40.0%
	GAAGCTTCTGAATCAGATCA+TGG	+	chr2.4:3985835-3985854	MS.gene85185:CDS	40.0%
	GGGAAACATCGTGAAATCTT+TGG	+	chr2.4:3986105-3986124	MS.gene85185:CDS	40.0%
	GTCAACAATCAGCAAATCGA+TGG	+	chr2.4:3986084-3986103	MS.gene85185:CDS	40.0%
	TGTGAATATGGAGGTTCAAG+CGG	+	chr2.4:3986195-3986214	MS.gene85185:CDS	40.0%
!!	GAAATCTTTGGAATCGTCGT+TGG	+	chr2.4:3986117-3986136	MS.gene85185:CDS	40.0%
!!	GGAGTTTATGGAAAGTGCTA+TGG	+	chr2.4:3986216-3986235	MS.gene85185:CDS	40.0%
!!	GTTTATGGAAAGTGCTATGG+CGG	+	chr2.4:3986219-3986238	MS.gene85185:CDS	40.0%
!!	TTGGCGTTGAAGAGATTTTG+AGG	-	chr2.4:3985873-3985892	None:intergenic	40.0%
!!	TTTATGGAAAGTGCTATGGC+GGG	+	chr2.4:3986220-3986239	MS.gene85185:CDS	40.0%
	CATCCAATCGGGAGGATAAT+CGG	-	chr2.4:3986030-3986049	None:intergenic	45.0%
	CTTGCTGAGCTTAAAGCTAG+AGG	+	chr2.4:3986412-3986431	MS.gene85185:CDS	45.0%
	GGAAGTTTCGGTGAATCTTC+CGG	+	chr2.4:3986315-3986334	MS.gene85185:CDS	45.0%
	GGGGAATTTGCAGAAGATGT+CGG	+	chr2.4:3986144-3986163	MS.gene85185:CDS	45.0%
	TAAGGAGAAGGATGCTGAGA+AGG	+	chr2.4:3986363-3986382	MS.gene85185:CDS	45.0%
	TAGTTTGATGCAACAGGTGG+CGG	+	chr2.4:3986279-3986298	MS.gene85185:CDS	45.0%
	TCAGCATCCTTCTCCTTAAC+CGG	-	chr2.4:3986361-3986380	None:intergenic	45.0%
!	TCATCTGCTCTGTGCGTTTT+CGG	-	chr2.4:3986000-3986019	None:intergenic	45.0%
	ACTTCGCTTTCGACTCCTGA+AGG	+	chr2.4:3986247-3986266	MS.gene85185:CDS	50.0%
	AGTTTCGGTGAATCTTCCGG+TGG	+	chr2.4:3986318-3986337	MS.gene85185:CDS	50.0%
	CTTCGCTTTCGACTCCTGAA+GGG	+	chr2.4:3986248-3986267	MS.gene85185:CDS	50.0%
	GGAGGTTCAAGCGGAGTTTA+TGG	+	chr2.4:3986204-3986223	MS.gene85185:CDS	50.0%
	TGCTCATGCTGTTCCGGTTA+AGG	+	chr2.4:3986345-3986364	MS.gene85185:CDS	50.0%
	TGCTGTTCCGGTTAAGGAGA+AGG	+	chr2.4:3986351-3986370	MS.gene85185:CDS	50.0%
	TGTATCGAGACGAGCACTCA+CGG	-	chr2.4:3986053-3986072	None:intergenic	50.0%
	TTCGCTTTCGACTCCTGAAG+GGG	+	chr2.4:3986249-3986268	MS.gene85185:CDS	50.0%
!	TTTGGAATCGTCGTTGGCGA+CGG	+	chr2.4:3986123-3986142	MS.gene85185:CDS	50.0%
!!	CAACAGGTGGCGGATGATTA+TGG	+	chr2.4:3986289-3986308	MS.gene85185:CDS	50.0%
!!	GGATGCTGAGAAGGTTGATG+AGG	+	chr2.4:3986372-3986391	MS.gene85185:CDS	50.0%
!!	GGTGGCGGATGATTATGGAT+TGG	+	chr2.4:3986294-3986313	MS.gene85185:CDS	50.0%
	AGCACTCACGGCATCCAATC+GGG	-	chr2.4:3986041-3986060	None:intergenic	55.0%
	CCAATCGGGAGGATAATCGG+AGG	-	chr2.4:3986027-3986046	None:intergenic	55.0%
	CCTCCGATTATCCTCCCGAT+TGG	+	chr2.4:3986024-3986043	MS.gene85185:CDS	55.0%
	GAGCACTCACGGCATCCAAT+CGG	-	chr2.4:3986042-3986061	None:intergenic	55.0%
	GAGTGCTCGTCTCGATACAC+AGG	+	chr2.4:3986054-3986073	MS.gene85185:CDS	55.0%
	GCAGAAGATGTCGGAGACGA+TGG	+	chr2.4:3986153-3986172	MS.gene85185:CDS	55.0%
	TTCGGTGAATCTTCCGGTGG+CGG	+	chr2.4:3986321-3986340	MS.gene85185:CDS	55.0%
	TTGGAATCGTCGTTGGCGAC+GGG	+	chr2.4:3986124-3986143	MS.gene85185:CDS	55.0%
	ACTCACGGCATCCAATCGGG+AGG	-	chr2.4:3986038-3986057	None:intergenic	60.0%
	GCTTTCGACTCCTGAAGGGG+AGG	+	chr2.4:3986252-3986271	MS.gene85185:CDS	60.0%
	TGGAATCGTCGTTGGCGACG+GGG	+	chr2.4:3986125-3986144	MS.gene85185:CDS	60.0%
	ACAGCATGAGCAGCCGCCAC+CGG	-	chr2.4:3986337-3986356	None:intergenic	65.0%
	GGCGGCTGCTCATGCTGTTC+CGG	+	chr2.4:3986339-3986358	MS.gene85185:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	3985821	3986438	3985821	ID=MS.gene85185
chr2.4	mRNA	3985821	3986438	3985821	ID=MS.gene85185.t1;Parent=MS.gene85185
chr2.4	exon	3985821	3986438	3985821	ID=MS.gene85185.t1.exon1;Parent=MS.gene85185.t1
chr2.4	CDS	3985821	3986438	3985821	ID=cds.MS.gene85185.t1;Parent=MS.gene85185.t1

Gene Sequence

>MS.gene85185

ATGGGAAACACAGAGAAGCTTCTGAATCAGATCATGGAATTGAAATTCACCTCAAAATCTCTTCAACGCCAAGCAAGAAAATGCGAAAAAGAAGAGAAATCCGAAAAACTTAAGGTGAAAAAAGCAATCGAAAAAGGTAACATGGACGGTGCACGAATCTACGCTGAAAACGCGATCCGAAAACGCACAGAGCAGATGAACTACCTCCGATTATCCTCCCGATTGGATGCCGTGAGTGCTCGTCTCGATACACAGGCGAAGATGTCAACAATCAGCAAATCGATGGGAAACATCGTGAAATCTTTGGAATCGTCGTTGGCGACGGGGAATTTGCAGAAGATGTCGGAGACGATGGATCAATTCGAGAAGCAGTTTGTGAATATGGAGGTTCAAGCGGAGTTTATGGAAAGTGCTATGGCGGGATCGACTTCGCTTTCGACTCCTGAAGGGGAGGTTAATAGTTTGATGCAACAGGTGGCGGATGATTATGGATTGGAAGTTTCGGTGAATCTTCCGGTGGCGGCTGCTCATGCTGTTCCGGTTAAGGAGAAGGATGCTGAGAAGGTTGATGAGGATGATTTGTCAAGGCGTCTTGCTGAGCTTAAAGCTAGAGGTTAA

Protein sequence

>MS.gene85185.t1

MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKVKKAIEKGNMDGARIYAENAIRKRTEQMNYLRLSSRLDAVSARLDTQAKMSTISKSMGNIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQAEFMESAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVNLPVAAAHAVPVKEKDAEKVDEDDLSRRLAELKARG