AlfalfaGEDB | MS.gene76575

M. sativa cultivar XinJiangDaYe / MS.gene76575

KEGG: K12197
GO: GO:0006810 GO:0007034 GO:0008150 GO:0051179 GO:0051234
PFAM: PF03357.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76575.t1	XP_003597636.1	100	205	0	0	1	205	1	205	2.90E-99	371.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76575.t1	Q8LE58	87.8	205	23	1	1	205	1	203	2.5e-88	326.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76575.t1	B7FHV8	100.0	205	0	0	1	205	1	205	2.1e-99	371.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053006	MS.gene76575	0.806312	8.74E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene76575	MS.gene36391	PPI
MS.gene76575	MS.gene85185	PPI
MS.gene76575	MS.gene069907	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76575.t1	MTR_2g100420	100.000	205	0	0	1	205	1	205	2.80e-150	415

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene76575.t1	AT1G17730	87.805	205	23	1	1	205	1	203	1.04e-130	366
MS.gene76575.t1	AT1G73030	88.293	205	22	1	1	205	1	203	8.49e-119	335
MS.gene76575.t1	AT2G06530	27.273	165	116	2	19	180	7	170	6.49e-12	62.4
MS.gene76575.t1	AT2G06530	27.273	165	116	2	19	180	28	191	8.56e-12	62.4

Find 57 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAACAGGTGGCGGATGATTA+TGG	0.176951	2.3:+3244392	MS.gene76575:CDS
GGAGGTTCAAGCGGAGTTTA+TGG	0.198154	2.3:+3244307	MS.gene76575:CDS
CGAGAAGCAGTTTGTGAATA+TGG	0.254306	2.3:+3244286	MS.gene76575:CDS
GTCGACAATCAGCAAATCTA+TGG	0.272483	2.3:+3244187	MS.gene76575:CDS
TCATCTGCTCTGTGCGTTTA+CGG	0.305607	2.3:-3244100	None:intergenic
GGAAGTTTCGGTTAATCTTC+CGG	0.355996	2.3:+3244418	MS.gene76575:CDS
TGATTATGGGCTGGAAGTTT+CGG	0.370615	2.3:+3244406	MS.gene76575:CDS
GGCGGCTGCTCATGCTGTTC+CGG	0.374454	2.3:+3244442	MS.gene76575:CDS
TGCTCATGCTGTTCCGGTTA+AGG	0.374890	2.3:+3244448	MS.gene76575:CDS
TCAGCATCCTTCTCCTTAAC+CGG	0.398938	2.3:-3244461	None:intergenic
GAGCACTCACGGCGTCCAAT+CGG	0.402083	2.3:-3244142	None:intergenic
CGTCCAATCGGGAGGATAAT+CGG	0.420046	2.3:-3244130	None:intergenic
CGACATCTTCTGCAAATTCC+CGG	0.428245	2.3:-3244246	None:intergenic
GGTTAATAGTTTGATGCAAC+AGG	0.440152	2.3:+3244376	MS.gene76575:CDS
AACAGGTGGCGGATGATTAT+GGG	0.452583	2.3:+3244393	MS.gene76575:CDS
TTCCCTTTCGACTCCGGAAG+GGG	0.455520	2.3:+3244352	MS.gene76575:CDS
TTCGGTTAATCTTCCGGTGG+CGG	0.462698	2.3:+3244424	MS.gene76575:CDS
ATCGACTTCCCTTTCGACTC+CGG	0.467684	2.3:+3244346	MS.gene76575:CDS
TTGGAATCGTCGTTGGCGAC+CGG	0.469029	2.3:+3244227	MS.gene76575:CDS
CTCCCCTTCCGGAGTCGAAA+GGG	0.473087	2.3:-3244354	None:intergenic
AAGCTTCTCTGTGTTTCCCA+TGG	0.476337	2.3:-3243922	None:intergenic
TAGTTTGATGCAACAGGTGG+CGG	0.477361	2.3:+3244382	MS.gene76575:CDS
AGCACTCACGGCGTCCAATC+GGG	0.483446	2.3:-3244141	None:intergenic
ACAGCATGAGCAGCCGCCAC+CGG	0.500440	2.3:-3244437	None:intergenic
TGGAATCGTCGTTGGCGACC+GGG	0.513207	2.3:+3244228	MS.gene76575:CDS
TGCTGTTCCGGTTAAGGAGA+AGG	0.523122	2.3:+3244454	MS.gene76575:CDS
CTTCCCTTTCGACTCCGGAA+GGG	0.524629	2.3:+3244351	MS.gene76575:CDS
AAACTATTAACCTCCCCTTC+CGG	0.527569	2.3:-3244365	None:intergenic
GGGAAACATCGTGAAATCGT+TGG	0.530824	2.3:+3244208	MS.gene76575:CDS
CCAAGCACGAAAATGCGAAA+AGG	0.531908	2.3:+3243992	MS.gene76575:CDS
GAAGCTTCTGAATCAGATCA+TGG	0.534967	2.3:+3243938	MS.gene76575:CDS
ACTTCCCTTTCGACTCCGGA+AGG	0.549210	2.3:+3244350	MS.gene76575:CDS
GCAGAAGATGTCGGAGACGA+TGG	0.561045	2.3:+3244256	MS.gene76575:CDS
TAAGGAGAAGGATGCTGAGA+AGG	0.573843	2.3:+3244466	MS.gene76575:CDS
TCGACAATCAGCAAATCTAT+GGG	0.575428	2.3:+3244188	MS.gene76575:CDS
CCTCCCCTTCCGGAGTCGAA+AGG	0.580228	2.3:-3244355	None:intergenic
GAAATCGTTGGAATCGTCGT+TGG	0.580522	2.3:+3244220	MS.gene76575:CDS
GGAGTTTATGGAAAGTGCTA+TGG	0.582336	2.3:+3244319	MS.gene76575:CDS
CCTTTCGACTCCGGAAGGGG+AGG	0.586411	2.3:+3244355	MS.gene76575:CDS
CTTGCTGAGCTTAAAGCTAG+AGG	0.589782	2.3:+3244515	MS.gene76575:CDS
GGTGGCGGATGATTATGGGC+TGG	0.595315	2.3:+3244397	MS.gene76575:CDS
GGATGCTGAGAAGGTTGATG+AGG	0.596267	2.3:+3244475	MS.gene76575:CDS
GTTTATGGAAAGTGCTATGG+CGG	0.596862	2.3:+3244322	MS.gene76575:CDS
TGTGAATATGGAGGTTCAAG+CGG	0.607620	2.3:+3244298	MS.gene76575:CDS
GAGTGCTCGTCTCGATACAC+AGG	0.608048	2.3:+3244157	MS.gene76575:CDS
AGCAATCGAGAAAGGTAACA+TGG	0.611081	2.3:+3244046	MS.gene76575:CDS
TGTATCGAGACGAGCACTCA+CGG	0.615716	2.3:-3244153	None:intergenic
CGGGAATTTGCAGAAGATGT+CGG	0.620775	2.3:+3244247	MS.gene76575:CDS
CCTCCGATTATCCTCCCGAT+TGG	0.622929	2.3:+3244127	MS.gene76575:CDS
ATCGAGAAAGGTAACATGGA+CGG	0.631489	2.3:+3244050	MS.gene76575:CDS
TTGATGAGGATGATTTGTCA+AGG	0.632184	2.3:+3244489	MS.gene76575:CDS
TTTATGGAAAGTGCTATGGC+GGG	0.633565	2.3:+3244323	MS.gene76575:CDS
TAATAGTTTGATGCAACAGG+TGG	0.645930	2.3:+3244379	MS.gene76575:CDS
ACTCACGGCGTCCAATCGGG+AGG	0.657435	2.3:-3244138	None:intergenic
GAAGCAGTTTGTGAATATGG+AGG	0.657749	2.3:+3244289	MS.gene76575:CDS
AGTTTCGGTTAATCTTCCGG+TGG	0.683279	2.3:+3244421	MS.gene76575:CDS
CCAATCGGGAGGATAATCGG+AGG	0.739296	2.3:-3244127	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTCACCTTGAGTTTTT+CGG	-	chr2.3:3244027-3244046	None:intergenic	25.0%
	AGAGAAATCCGAAAAACTCA+AGG	+	chr2.3:3244016-3244035	MS.gene76575:CDS	35.0%
	GGTTAATAGTTTGATGCAAC+AGG	+	chr2.3:3244376-3244395	MS.gene76575:CDS	35.0%
	GTGAAAAAAGCAATCGAGAA+AGG	+	chr2.3:3244038-3244057	MS.gene76575:CDS	35.0%
	TAATAGTTTGATGCAACAGG+TGG	+	chr2.3:3244379-3244398	MS.gene76575:CDS	35.0%
	TCGACAATCAGCAAATCTAT+GGG	+	chr2.3:3244188-3244207	MS.gene76575:CDS	35.0%
!	TTGATGAGGATGATTTGTCA+AGG	+	chr2.3:3244489-3244508	MS.gene76575:CDS	35.0%
	AAACTATTAACCTCCCCTTC+CGG	-	chr2.3:3244368-3244387	None:intergenic	40.0%
	AGCAATCGAGAAAGGTAACA+TGG	+	chr2.3:3244046-3244065	MS.gene76575:CDS	40.0%
	ATCGAGAAAGGTAACATGGA+CGG	+	chr2.3:3244050-3244069	MS.gene76575:CDS	40.0%
	CGAGAAGCAGTTTGTGAATA+TGG	+	chr2.3:3244286-3244305	MS.gene76575:CDS	40.0%
	GAAGCAGTTTGTGAATATGG+AGG	+	chr2.3:3244289-3244308	MS.gene76575:CDS	40.0%
	GAAGCTTCTGAATCAGATCA+TGG	+	chr2.3:3243938-3243957	MS.gene76575:CDS	40.0%
	GGAAGTTTCGGTTAATCTTC+CGG	+	chr2.3:3244418-3244437	MS.gene76575:CDS	40.0%
	GTCGACAATCAGCAAATCTA+TGG	+	chr2.3:3244187-3244206	MS.gene76575:CDS	40.0%
	TGTGAATATGGAGGTTCAAG+CGG	+	chr2.3:3244298-3244317	MS.gene76575:CDS	40.0%
!!	GGAGTTTATGGAAAGTGCTA+TGG	+	chr2.3:3244319-3244338	MS.gene76575:CDS	40.0%
!!	GTTTATGGAAAGTGCTATGG+CGG	+	chr2.3:3244322-3244341	MS.gene76575:CDS	40.0%
!!	TGATTATGGGCTGGAAGTTT+CGG	+	chr2.3:3244406-3244425	MS.gene76575:CDS	40.0%
!!	TTGGCGTTGAAGAGATTTTG+AGG	-	chr2.3:3243976-3243995	None:intergenic	40.0%
!!	TTTATGGAAAGTGCTATGGC+GGG	+	chr2.3:3244323-3244342	MS.gene76575:CDS	40.0%
	AGTTTCGGTTAATCTTCCGG+TGG	+	chr2.3:3244421-3244440	MS.gene76575:CDS	45.0%
	CCAAGCACGAAAATGCGAAA+AGG	+	chr2.3:3243992-3244011	MS.gene76575:CDS	45.0%
	CGACATCTTCTGCAAATTCC+CGG	-	chr2.3:3244249-3244268	None:intergenic	45.0%
	CGGGAATTTGCAGAAGATGT+CGG	+	chr2.3:3244247-3244266	MS.gene76575:CDS	45.0%
	CTTGCTGAGCTTAAAGCTAG+AGG	+	chr2.3:3244515-3244534	MS.gene76575:CDS	45.0%
	GAAATCGTTGGAATCGTCGT+TGG	+	chr2.3:3244220-3244239	MS.gene76575:CDS	45.0%
	GGGAAACATCGTGAAATCGT+TGG	+	chr2.3:3244208-3244227	MS.gene76575:CDS	45.0%
	TAAGGAGAAGGATGCTGAGA+AGG	+	chr2.3:3244466-3244485	MS.gene76575:CDS	45.0%
	TAGTTTGATGCAACAGGTGG+CGG	+	chr2.3:3244382-3244401	MS.gene76575:CDS	45.0%
	TCAGCATCCTTCTCCTTAAC+CGG	-	chr2.3:3244464-3244483	None:intergenic	45.0%
	TCATCTGCTCTGTGCGTTTA+CGG	-	chr2.3:3244103-3244122	None:intergenic	45.0%
!	CCTTTTCGCATTTTCGTGCT+TGG	-	chr2.3:3243995-3244014	None:intergenic	45.0%
!!	AACAGGTGGCGGATGATTAT+GGG	+	chr2.3:3244393-3244412	MS.gene76575:CDS	45.0%
	CGTCCAATCGGGAGGATAAT+CGG	-	chr2.3:3244133-3244152	None:intergenic	50.0%
	GGAGGTTCAAGCGGAGTTTA+TGG	+	chr2.3:3244307-3244326	MS.gene76575:CDS	50.0%
	TGCTCATGCTGTTCCGGTTA+AGG	+	chr2.3:3244448-3244467	MS.gene76575:CDS	50.0%
	TGCTGTTCCGGTTAAGGAGA+AGG	+	chr2.3:3244454-3244473	MS.gene76575:CDS	50.0%
	TGTATCGAGACGAGCACTCA+CGG	-	chr2.3:3244156-3244175	None:intergenic	50.0%
	TTCGGTTAATCTTCCGGTGG+CGG	+	chr2.3:3244424-3244443	MS.gene76575:CDS	50.0%
!	ATCGACTTCCCTTTCGACTC+CGG	+	chr2.3:3244346-3244365	MS.gene76575:CDS	50.0%
!!	CAACAGGTGGCGGATGATTA+TGG	+	chr2.3:3244392-3244411	MS.gene76575:CDS	50.0%
!!	GGATGCTGAGAAGGTTGATG+AGG	+	chr2.3:3244475-3244494	MS.gene76575:CDS	50.0%
	ACTTCCCTTTCGACTCCGGA+AGG	+	chr2.3:3244350-3244369	MS.gene76575:CDS	55.0%
	CCAATCGGGAGGATAATCGG+AGG	-	chr2.3:3244130-3244149	None:intergenic	55.0%
	CCTCCGATTATCCTCCCGAT+TGG	+	chr2.3:3244127-3244146	MS.gene76575:CDS	55.0%
	CTTCCCTTTCGACTCCGGAA+GGG	+	chr2.3:3244351-3244370	MS.gene76575:CDS	55.0%
	GAGTGCTCGTCTCGATACAC+AGG	+	chr2.3:3244157-3244176	MS.gene76575:CDS	55.0%
	GCAGAAGATGTCGGAGACGA+TGG	+	chr2.3:3244256-3244275	MS.gene76575:CDS	55.0%
	TTCCCTTTCGACTCCGGAAG+GGG	+	chr2.3:3244352-3244371	MS.gene76575:CDS	55.0%
	TTGGAATCGTCGTTGGCGAC+CGG	+	chr2.3:3244227-3244246	MS.gene76575:CDS	55.0%
	AGCACTCACGGCGTCCAATC+GGG	-	chr2.3:3244144-3244163	None:intergenic	60.0%
	CTCCCCTTCCGGAGTCGAAA+GGG	-	chr2.3:3244357-3244376	None:intergenic	60.0%
	GAGCACTCACGGCGTCCAAT+CGG	-	chr2.3:3244145-3244164	None:intergenic	60.0%
	TGGAATCGTCGTTGGCGACC+GGG	+	chr2.3:3244228-3244247	MS.gene76575:CDS	60.0%
!!	GGTGGCGGATGATTATGGGC+TGG	+	chr2.3:3244397-3244416	MS.gene76575:CDS	60.0%
	ACAGCATGAGCAGCCGCCAC+CGG	-	chr2.3:3244440-3244459	None:intergenic	65.0%
	ACTCACGGCGTCCAATCGGG+AGG	-	chr2.3:3244141-3244160	None:intergenic	65.0%
	CCTCCCCTTCCGGAGTCGAA+AGG	-	chr2.3:3244358-3244377	None:intergenic	65.0%
	CCTTTCGACTCCGGAAGGGG+AGG	+	chr2.3:3244355-3244374	MS.gene76575:CDS	65.0%
	GGCGGCTGCTCATGCTGTTC+CGG	+	chr2.3:3244442-3244461	MS.gene76575:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	3243924	3244541	3243924	ID=MS.gene76575
chr2.3	mRNA	3243924	3244541	3243924	ID=MS.gene76575.t1;Parent=MS.gene76575
chr2.3	exon	3243924	3244541	3243924	ID=MS.gene76575.t1.exon1;Parent=MS.gene76575.t1
chr2.3	CDS	3243924	3244541	3243924	ID=cds.MS.gene76575.t1;Parent=MS.gene76575.t1

Gene Sequence

>MS.gene76575

ATGGGAAACACAGAGAAGCTTCTGAATCAGATCATGGAATTGAAATTCACCTCAAAATCTCTTCAACGCCAAGCACGAAAATGCGAAAAGGAAGAGAAATCCGAAAAACTCAAGGTGAAAAAAGCAATCGAGAAAGGTAACATGGACGGTGCACGAATCTACGCTGAAAACGCGATCCGTAAACGCACAGAGCAGATGAACTACCTCCGATTATCCTCCCGATTGGACGCCGTGAGTGCTCGTCTCGATACACAGGCAAAGATGTCGACAATCAGCAAATCTATGGGAAACATCGTGAAATCGTTGGAATCGTCGTTGGCGACCGGGAATTTGCAGAAGATGTCGGAGACGATGGATCAATTCGAGAAGCAGTTTGTGAATATGGAGGTTCAAGCGGAGTTTATGGAAAGTGCTATGGCGGGATCGACTTCCCTTTCGACTCCGGAAGGGGAGGTTAATAGTTTGATGCAACAGGTGGCGGATGATTATGGGCTGGAAGTTTCGGTTAATCTTCCGGTGGCGGCTGCTCATGCTGTTCCGGTTAAGGAGAAGGATGCTGAGAAGGTTGATGAGGATGATTTGTCAAGGCGTCTTGCTGAGCTTAAAGCTAGAGGTTAA

Protein sequence

>MS.gene76575.t1

MGNTEKLLNQIMELKFTSKSLQRQARKCEKEEKSEKLKVKKAIEKGNMDGARIYAENAIRKRTEQMNYLRLSSRLDAVSARLDTQAKMSTISKSMGNIVKSLESSLATGNLQKMSETMDQFEKQFVNMEVQAEFMESAMAGSTSLSTPEGEVNSLMQQVADDYGLEVSVNLPVAAAHAVPVKEKDAEKVDEDDLSRRLAELKARG