AlfalfaGEDB | MS.gene85756

KEGG: -
GO: -
PFAM: PF04756.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85756.t1	XP_003595113.1	98.9	88	1	0	1	88	88	175	2.70E-38	167.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85756.t1	Q5ZJR3	42.0	81	47	0	7	87	67	147	1.4e-11	70.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85756.t1	G7IIC1	98.9	88	1	0	1	88	88	175	1.9e-38	167.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04991	MS.gene85756	0.84533	4.34E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85756.t1	MTR_2g038390	98.864	88	1	0	1	88	88	175	2.06e-57	174

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85756.t1	AT4G29870	86.047	86	12	2	87	86	171	2.53e-49	154
MS.gene85756.t1	AT2G19340	81.395	86	16	2	87	87	172	2.60e-47	148
MS.gene85756.t1	AT2G19340	81.081	74	14	2	75	87	160	4.59e-39	128

Find 25 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTTCGTGCTTGGTGGAAT+TGG	0.312895	2.4:+51048834	MS.gene85756:CDS
ATCTTGCTCTTGATCGAAAC+CGG	0.359805	2.4:+51048871	MS.gene85756:CDS
TGGCTTAACGGCGCCGGTGT+AGG	0.406356	2.4:-51048748	None:intergenic
CATGAAGACGACTGGCTTAA+CGG	0.420596	2.4:-51048760	None:intergenic
TGTTCGTGCTTGGTGGAATT+GGG	0.427782	2.4:+51048835	MS.gene85756:CDS
TTCATGTCCGGACGTGTTAA+CGG	0.438277	2.4:+51048777	MS.gene85756:CDS
GGCTTAACGGCGCCGGTGTA+GGG	0.445049	2.4:-51048747	None:intergenic
ATCATTGAAGGTCTCTCGTC+AGG	0.449642	2.4:+51048807	MS.gene85756:CDS
TCAGGATTCATGTTCGTGCT+TGG	0.455117	2.4:+51048825	MS.gene85756:CDS
TGGAATTGGGATTATTATGC+TGG	0.456292	2.4:+51048848	MS.gene85756:CDS
GGATTCATGTTCGTGCTTGG+TGG	0.470744	2.4:+51048828	MS.gene85756:CDS
AAGCCAGTCGTCTTCATGTC+CGG	0.477097	2.4:+51048765	MS.gene85756:CDS
AAGGTTTCTTATGCAACTGC+TGG	0.491757	2.4:+51048906	MS.gene85756:CDS
CAAATTCAACTAAGATAAGC+CGG	0.499212	2.4:-51048987	None:intergenic
AAACCTTGACAGAGCGATCC+CGG	0.507272	2.4:-51048890	None:intergenic
GACGACTGGCTTAACGGCGC+CGG	0.515557	2.4:-51048754	None:intergenic
CTTCGTTCGTATCAAGATCC+CGG	0.526209	2.4:+51048968	MS.gene85756:CDS
AACGGTCAGTACATCATTGA+AGG	0.541670	2.4:+51048795	MS.gene85756:CDS
GTACTGACCGTTAACACGTC+CGG	0.550984	2.4:-51048784	None:intergenic
AAACCGGGATCGCTCTGTCA+AGG	0.555090	2.4:+51048887	MS.gene85756:CDS
TCTTGCTCTTGATCGAAACC+GGG	0.589186	2.4:+51048872	MS.gene85756:CDS
AAATTCAACTAAGATAAGCC+GGG	0.596011	2.4:-51048986	None:intergenic
CGTCCGGACATGAAGACGAC+TGG	0.599107	2.4:-51048768	None:intergenic
AACATAAGCAATGACAACAC+TGG	0.647800	2.4:-51048934	None:intergenic
TCAATGCAAGATCCCTACAC+CGG	0.666564	2.4:+51048735	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACATAAGCAATGACAACAC+TGG	-	chr2.4:51048937-51048956	None:intergenic	35.0%
	TGGAATTGGGATTATTATGC+TGG	+	chr2.4:51048848-51048867	MS.gene85756:CDS	35.0%
	AACGGTCAGTACATCATTGA+AGG	+	chr2.4:51048795-51048814	MS.gene85756:CDS	40.0%
	AAGGTTTCTTATGCAACTGC+TGG	+	chr2.4:51048906-51048925	MS.gene85756:CDS	40.0%
	ATCTTGCTCTTGATCGAAAC+CGG	+	chr2.4:51048871-51048890	MS.gene85756:CDS	40.0%
	ATCATTGAAGGTCTCTCGTC+AGG	+	chr2.4:51048807-51048826	MS.gene85756:CDS	45.0%
	CTTCGTTCGTATCAAGATCC+CGG	+	chr2.4:51048968-51048987	MS.gene85756:CDS	45.0%
	TCAGGATTCATGTTCGTGCT+TGG	+	chr2.4:51048825-51048844	MS.gene85756:CDS	45.0%
	TCTTGCTCTTGATCGAAACC+GGG	+	chr2.4:51048872-51048891	MS.gene85756:CDS	45.0%
	TTCATGTCCGGACGTGTTAA+CGG	+	chr2.4:51048777-51048796	MS.gene85756:CDS	45.0%
!	ATGTTCGTGCTTGGTGGAAT+TGG	+	chr2.4:51048834-51048853	MS.gene85756:CDS	45.0%
!	TGTTCGTGCTTGGTGGAATT+GGG	+	chr2.4:51048835-51048854	MS.gene85756:CDS	45.0%
!!	CATGAAGACGACTGGCTTAA+CGG	-	chr2.4:51048763-51048782	None:intergenic	45.0%
	AAACCTTGACAGAGCGATCC+CGG	-	chr2.4:51048893-51048912	None:intergenic	50.0%
	AAGCCAGTCGTCTTCATGTC+CGG	+	chr2.4:51048765-51048784	MS.gene85756:CDS	50.0%
	GGATTCATGTTCGTGCTTGG+TGG	+	chr2.4:51048828-51048847	MS.gene85756:CDS	50.0%
!	GTACTGACCGTTAACACGTC+CGG	-	chr2.4:51048787-51048806	None:intergenic	50.0%
	AAACCGGGATCGCTCTGTCA+AGG	+	chr2.4:51048887-51048906	MS.gene85756:CDS	55.0%
	CGTCCGGACATGAAGACGAC+TGG	-	chr2.4:51048771-51048790	None:intergenic	60.0%
!!	GACGACTGGCTTAACGGCGC+CGG	-	chr2.4:51048757-51048776	None:intergenic	65.0%
!!	GGCTTAACGGCGCCGGTGTA+GGG	-	chr2.4:51048750-51048769	None:intergenic	65.0%
!!	TGGCTTAACGGCGCCGGTGT+AGG	-	chr2.4:51048751-51048770	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	51048738	51049004	51048738	ID=MS.gene85756
chr2.4	mRNA	51048738	51049004	51048738	ID=MS.gene85756.t1;Parent=MS.gene85756
chr2.4	exon	51048738	51049004	51048738	ID=MS.gene85756.t1.exon1;Parent=MS.gene85756.t1
chr2.4	CDS	51048738	51049004	51048738	ID=cds.MS.gene85756.t1;Parent=MS.gene85756.t1

Gene Sequence	>MS.gene85756 ATGCAAGATCCCTACACCGGCGCCGTTAAGCCAGTCGTCTTCATGTCCGGACGTGTTAACGGTCAGTACATCATTGAAGGTCTCTCGTCAGGATTCATGTTCGTGCTTGGTGGAATTGGGATTATTATGCTGGATCTTGCTCTTGATCGAAACCGGGATCGCTCTGTCAAGGTTTCTTATGCAACTGCTGGTATTACCAGTGTTGTCATTGCTTATGTTATGAGTATGCTCTTCGTTCGTATCAAGATCCCGGCTTATCTTAGTTGA
Protein sequence	>MS.gene85756.t1 MQDPYTGAVKPVVFMSGRVNGQYIIEGLSSGFMFVLGGIGIIMLDLALDRNRDRSVKVSYATAGITSVVIAYVMSMLFVRIKIPAYLS

M. sativa cultivar XinJiangDaYe / MS.gene85756