AlfalfaGEDB | MS.gene85759

M. sativa cultivar XinJiangDaYe / MS.gene85759

KEGG: K23802
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85759.t1	RHN68548.1	90.9	33	3	0	1	33	400	432	1.90E-09	71.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85759.t1	A0A072UZN1	90.9	33	3	0	1	33	4	36	1.4e-09	71.6

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049853	MS.gene85759	0.818903	1.54E-52	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85759.t1	MTR_3g073080	90.909	33	3	1	33	472	504	1.48e-16	72.8
MS.gene85759.t1	MTR_3g073080	90.909	33	3	1	33	208	240	2.10e-16	72.4
MS.gene85759.t1	MTR_3g073080	90.909	33	3	1	33	4	36	2.96e-16	72.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 18 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCACTCTTCCCAATGCCGG+AGG	0.658673	1.2:-32888292	MS.gene85759:CDS
AGTTTATACGACGTATACAC+AGG	0.665776	1.2:-32887048	MS.gene85759:CDS
ATATATCTGACTGCACAAAT+AGG	0.395800	1.2:+32887004	None:intergenic
ATCCCAATTATGTGTGCATA+GGG	0.512882	1.2:+32888320	None:intergenic
ATGCACACATAATTGGGATA+TGG	0.373855	1.2:-32888316	MS.gene85759:CDS
CAGATATATCTATTGTAATG+AGG	0.568090	1.2:-32886990	MS.gene85759:CDS
CAGGTGCCGATAGAAGTGCA+TGG	0.677112	1.2:-32887029	MS.gene85759:CDS
CTGCATCTGCCTCCGGCATT+GGG	0.356862	1.2:+32888283	None:intergenic
GAGGCTCTTCAACTACATTT+AGG	0.281000	1.2:-32886971	None:intergenic
GCCTCCGGCATTGGGAAGAG+TGG	0.566459	1.2:+32888291	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTGACATCAGTTAAAGGA+AGG	+	chr1.2:32887422-32887441	None:intergenic	30.0%
AGAATGTGTTCAATGTTTGA+TGG	-	chr1.2:32887560-32887579	MS.gene85759:intron	30.0%
ATATACTCCAGTAAAAGAAC+TGG	+	chr1.2:32887195-32887214	None:intergenic	30.0%
ATATATCTGACTGCACAAAT+AGG	+	chr1.2:32888319-32888338	None:intergenic	30.0%
TATACTCCAGTAAAAGAACT+GGG	+	chr1.2:32887194-32887213	None:intergenic	30.0%
TTGCAGATAATTCATATCGT+TGG	-	chr1.2:32887810-32887829	MS.gene85759:intron	30.0%
AAGAGAGTGACATGTCAATT+CGG	+	chr1.2:32887098-32887117	None:intergenic	35.0%
ACTTTCAGTGTATTGAGTCT+AGG	-	chr1.2:32887881-32887900	MS.gene85759:intron	35.0%
AGTTTATACGACGTATACAC+AGG	-	chr1.2:32888272-32888291	MS.gene85759:intron	35.0%
ATACTCCAGTAAAAGAACTG+GGG	+	chr1.2:32887193-32887212	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	32886972	32888370	32886972	ID=MS.gene85759
chr1.2	mRNA	32886972	32888370	32886972	ID=MS.gene85759.t1;Parent=MS.gene85759
chr1.2	exon	32888273	32888370	32888273	ID=MS.gene85759.t1.exon1;Parent=MS.gene85759.t1
chr1.2	CDS	32888273	32888370	32888273	ID=cds.MS.gene85759.t1;Parent=MS.gene85759.t1
chr1.2	exon	32887188	32887239	32887188	ID=MS.gene85759.t1.exon2;Parent=MS.gene85759.t1
chr1.2	CDS	32887188	32887239	32887188	ID=cds.MS.gene85759.t1;Parent=MS.gene85759.t1
chr1.2	exon	32886972	32887076	32886972	ID=MS.gene85759.t1.exon3;Parent=MS.gene85759.t1
chr1.2	CDS	32886972	32887076	32886972	ID=cds.MS.gene85759.t1;Parent=MS.gene85759.t1

Gene Sequence

>MS.gene85759

ATGATCACCAAGGCTACAGCTGATTGTACCCTATGCACACATAATTGGGATATGGAACCACTCTTCCCAATGCCGGAGGCAGATGCAGACAATAAAGAGTAAGTTGTTTCTTGTTTACCTTCTCCGAATTGACATGTCACTCTCTTTATTAGTTTGTGAACTTCAGGGGCTTATGATTTTCACTGATCTTCAAGATTTGATCAATGTAGTGTTCCTTCCCCCAGTTCTTTTACTGGAGTATATATAAGTACGCATGTAAGAAGCTATAGGATTTTAGATGTTATCCAATATTAAATAGTTTTGATTCAATTGAGACGGTCTGGAGTGGCTCACCTTTGTTTCTATTACAGAGATACTATTAATATTTTCCCCAAAGAGTATGGTTTTTAAAATCTTTAGTGGTGCAAAGCACTTGTAGTTATACATAAATTTGCAAAGTATTTGTATCCTTCCTTTAACTGATGTCAATTAATATTTGTAGTTATGCATTCTGGGTGTTTGGACAAACAATCTTTCCAAATAGTGTTCAATTATCCCCTTTCTTCTTGAAGCTCTACATAGACATGACAAGATTTGTATATGTATATGAGAATGTGTTCAATGTTTGATGGTGAATAACAAATGATTTCATACTTATAATTTGCAATGGAAAGAAACTAGTGAGTTTCTATGTGTTGAGTGTGTTGAACTGTTAAACCACTACAAGAAATTTAGCATTTTGCCCGGGTTTCTGTTTTAGGCAATGTTTGGTATCTATTGCTGTTGGAGCAGGCAATTTTGGATTTTGCAGAAGGCCTAAGAGTTATTGGAGTAAGGCCAGATGAAAAGCTTGCACTTTTTGCAGATAATTCATATCGTTGGCTAGTTGCAGATCAAGGTGTGTCAATCTCTTTTTTCTTTATCTTTTCCACTTTCAGTGTATTGAGTCTAGGAATTGCCAGTTGCATAAATCTCACGTGTGAAGGAAGTGTAATTTTACTATCAGTGTAATTTTCAACACACTATTTGGCCTTTGCACAAGCTTTTGGATGACTGTTATAATGAGATCTGTTTTTGGAGGTTTAAATGGTTTGCTTGGACTAATGAATGTATCACTCAAAACACTATACTAGCATTATCCTTCATTAATAGGCTTATTCTTCTGAAATCTGCAGAGAAGAATACTTGTGATATGTCTGATGCAGTATGCTTCAATTCTTGGTCTTTCTAGCATTAGTTTTTCTAGAATGTCTTGTGATTTTTTATTGTATGTTTATACTGTTATTTTTTTTTTATAATTGAGTATCATTGAAAGGTGCATAGTTTATACGACGTATACACAGGTGCCGATAGAAGTGCATGGATCCTATTTGTGCAGTCAGATATATCTATTGTAATGAGGCTCTTCAACTACATTTAG

Protein sequence

>MS.gene85759.t1

MITKATADCTLCTHNWDMEPLFPMPEADADNKELILLKSAEKNTCDMSDAVHSLYDVYTGADRSAWILFVQSDISIVMRLFNYI