AlfalfaGEDB | MS.gene85774

KEGG: -
GO: -
PFAM: PF04756.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85774.t1	XP_003595113.1	98.9	88	1	0	1	88	88	175	2.70E-38	167.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85774.t1	Q5ZJR3	42.0	81	47	0	7	87	67	147	1.4e-11	70.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85774.t1	G7IIC1	98.9	88	1	0	1	88	88	175	1.9e-38	167.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04991	MS.gene85774	0.830323	3.13E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85774.t1	MTR_2g038390	98.864	88	1	0	1	88	88	175	2.06e-57	174

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85774.t1	AT4G29870	86.047	86	12	2	87	86	171	2.53e-49	154
MS.gene85774.t1	AT2G19340	81.395	86	16	2	87	87	172	2.60e-47	148
MS.gene85774.t1	AT2G19340	81.081	74	14	2	75	87	160	4.59e-39	128

Find 25 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTTCGTGCTTGGTGGAAT+TGG	0.312895	2.4:+51093125	MS.gene85774:CDS
ATCTTGCTCTTGATCGAAAC+CGG	0.359805	2.4:+51093162	MS.gene85774:CDS
TGGCTTAACGGCGCCGGTGT+AGG	0.406356	2.4:-51093039	None:intergenic
CATGAAGACGACTGGCTTAA+CGG	0.420596	2.4:-51093051	None:intergenic
TGTTCGTGCTTGGTGGAATT+GGG	0.427782	2.4:+51093126	MS.gene85774:CDS
TTCATGTCCGGACGTGTTAA+CGG	0.438277	2.4:+51093068	MS.gene85774:CDS
GGCTTAACGGCGCCGGTGTA+GGG	0.445049	2.4:-51093038	None:intergenic
ATCATTGAAGGTCTCTCGTC+AGG	0.449642	2.4:+51093098	MS.gene85774:CDS
TCAGGATTCATGTTCGTGCT+TGG	0.455117	2.4:+51093116	MS.gene85774:CDS
TGGAATTGGGATTATTATGC+TGG	0.456292	2.4:+51093139	MS.gene85774:CDS
GGATTCATGTTCGTGCTTGG+TGG	0.470744	2.4:+51093119	MS.gene85774:CDS
AAGCCAGTCGTCTTCATGTC+CGG	0.477097	2.4:+51093056	MS.gene85774:CDS
AAGGTTTCTTATGCAACTGC+TGG	0.491757	2.4:+51093197	MS.gene85774:CDS
CAAATTCAACTAAGATAAGC+CGG	0.499212	2.4:-51093278	None:intergenic
AAACCTTGACAGAGCGATCC+CGG	0.507272	2.4:-51093181	None:intergenic
GACGACTGGCTTAACGGCGC+CGG	0.515557	2.4:-51093045	None:intergenic
CTTCGTTCGTATCAAGATCC+CGG	0.526209	2.4:+51093259	MS.gene85774:CDS
AACGGTCAGTACATCATTGA+AGG	0.541670	2.4:+51093086	MS.gene85774:CDS
GTACTGACCGTTAACACGTC+CGG	0.550984	2.4:-51093075	None:intergenic
AAACCGGGATCGCTCTGTCA+AGG	0.555090	2.4:+51093178	MS.gene85774:CDS
TCTTGCTCTTGATCGAAACC+GGG	0.589186	2.4:+51093163	MS.gene85774:CDS
AAATTCAACTAAGATAAGCC+GGG	0.596011	2.4:-51093277	None:intergenic
CGTCCGGACATGAAGACGAC+TGG	0.599107	2.4:-51093059	None:intergenic
AACATAAGCAATGACAACAC+TGG	0.647800	2.4:-51093225	None:intergenic
TCAATGCAAGATCCCTACAC+CGG	0.666564	2.4:+51093026	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACATAAGCAATGACAACAC+TGG	-	chr2.4:51093228-51093247	None:intergenic	35.0%
	TGGAATTGGGATTATTATGC+TGG	+	chr2.4:51093139-51093158	MS.gene85774:CDS	35.0%
	AACGGTCAGTACATCATTGA+AGG	+	chr2.4:51093086-51093105	MS.gene85774:CDS	40.0%
	AAGGTTTCTTATGCAACTGC+TGG	+	chr2.4:51093197-51093216	MS.gene85774:CDS	40.0%
	ATCTTGCTCTTGATCGAAAC+CGG	+	chr2.4:51093162-51093181	MS.gene85774:CDS	40.0%
	ATCATTGAAGGTCTCTCGTC+AGG	+	chr2.4:51093098-51093117	MS.gene85774:CDS	45.0%
	CTTCGTTCGTATCAAGATCC+CGG	+	chr2.4:51093259-51093278	MS.gene85774:CDS	45.0%
	TCAGGATTCATGTTCGTGCT+TGG	+	chr2.4:51093116-51093135	MS.gene85774:CDS	45.0%
	TCTTGCTCTTGATCGAAACC+GGG	+	chr2.4:51093163-51093182	MS.gene85774:CDS	45.0%
	TTCATGTCCGGACGTGTTAA+CGG	+	chr2.4:51093068-51093087	MS.gene85774:CDS	45.0%
!	ATGTTCGTGCTTGGTGGAAT+TGG	+	chr2.4:51093125-51093144	MS.gene85774:CDS	45.0%
!	TGTTCGTGCTTGGTGGAATT+GGG	+	chr2.4:51093126-51093145	MS.gene85774:CDS	45.0%
!!	CATGAAGACGACTGGCTTAA+CGG	-	chr2.4:51093054-51093073	None:intergenic	45.0%
	AAACCTTGACAGAGCGATCC+CGG	-	chr2.4:51093184-51093203	None:intergenic	50.0%
	AAGCCAGTCGTCTTCATGTC+CGG	+	chr2.4:51093056-51093075	MS.gene85774:CDS	50.0%
	GGATTCATGTTCGTGCTTGG+TGG	+	chr2.4:51093119-51093138	MS.gene85774:CDS	50.0%
!	GTACTGACCGTTAACACGTC+CGG	-	chr2.4:51093078-51093097	None:intergenic	50.0%
	AAACCGGGATCGCTCTGTCA+AGG	+	chr2.4:51093178-51093197	MS.gene85774:CDS	55.0%
	CGTCCGGACATGAAGACGAC+TGG	-	chr2.4:51093062-51093081	None:intergenic	60.0%
!!	GACGACTGGCTTAACGGCGC+CGG	-	chr2.4:51093048-51093067	None:intergenic	65.0%
!!	GGCTTAACGGCGCCGGTGTA+GGG	-	chr2.4:51093041-51093060	None:intergenic	65.0%
!!	TGGCTTAACGGCGCCGGTGT+AGG	-	chr2.4:51093042-51093061	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	51093029	51093295	51093029	ID=MS.gene85774
chr2.4	mRNA	51093029	51093295	51093029	ID=MS.gene85774.t1;Parent=MS.gene85774
chr2.4	exon	51093029	51093295	51093029	ID=MS.gene85774.t1.exon1;Parent=MS.gene85774.t1
chr2.4	CDS	51093029	51093295	51093029	ID=cds.MS.gene85774.t1;Parent=MS.gene85774.t1

Gene Sequence	>MS.gene85774 ATGCAAGATCCCTACACCGGCGCCGTTAAGCCAGTCGTCTTCATGTCCGGACGTGTTAACGGTCAGTACATCATTGAAGGTCTCTCGTCAGGATTCATGTTCGTGCTTGGTGGAATTGGGATTATTATGCTGGATCTTGCTCTTGATCGAAACCGGGATCGCTCTGTCAAGGTTTCTTATGCAACTGCTGGTATTACCAGTGTTGTCATTGCTTATGTTATGAGTATGCTCTTCGTTCGTATCAAGATCCCGGCTTATCTTAGTTGA
Protein sequence	>MS.gene85774.t1 MQDPYTGAVKPVVFMSGRVNGQYIIEGLSSGFMFVLGGIGIIMLDLALDRNRDRSVKVSYATAGITSVVIAYVMSMLFVRIKIPAYLS

M. sativa cultivar XinJiangDaYe / MS.gene85774