Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85852.t1 | XP_013451990.1 | 89.9 | 119 | 11 | 1 | 1 | 118 | 1 | 119 | 2.10E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85852.t1 | G7ZYN0 | 89.9 | 119 | 11 | 1 | 1 | 118 | 1 | 119 | 1.5e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058496 | MS.gene85852 | 0.813168 | 2.93E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85852.t1 | MTR_6g039830 | 89.916 | 119 | 11 | 1 | 1 | 118 | 1 | 119 | 3.65e-76 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene85852.t1 | AT1G71740 | 48.000 | 125 | 49 | 3 | 10 | 118 | 6 | 130 | 1.86e-31 | 108 |
| MS.gene85852.t1 | AT1G49000 | 61.111 | 36 | 14 | 0 | 71 | 106 | 102 | 137 | 7.33e-11 | 56.2 |
Find 29 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTACTTACATGGAAATA+AGG | 0.247585 | 6.4:-15155604 | None:intergenic |
| TTGGCAGAAGAATATACTTA+TGG | 0.292425 | 6.4:-15155713 | MS.gene85852:CDS |
| TCACTAAGCACATCAAATAT+TGG | 0.309384 | 6.4:-15155910 | MS.gene85852:CDS |
| TCATTGTTTAGCTCTATAAC+AGG | 0.379640 | 6.4:+15155781 | None:intergenic |
| GCTCGCTTGCAATGCCTTTG+CGG | 0.390954 | 6.4:-15155809 | MS.gene85852:CDS |
| CTTCTCTTCCATTACTTACA+TGG | 0.403788 | 6.4:-15155612 | MS.gene85852:CDS |
| TGGCAGAAGAATATACTTAT+GGG | 0.423192 | 6.4:-15155712 | MS.gene85852:CDS |
| GTGCTTCTCATTCGCGCAAA+TGG | 0.423947 | 6.4:-15155840 | MS.gene85852:CDS |
| ATTCGCGCAAATGGCGCCAT+TGG | 0.444761 | 6.4:-15155831 | MS.gene85852:CDS |
| TGTCAGCTCCCTGATTTCTC+TGG | 0.448550 | 6.4:-15155682 | MS.gene85852:CDS |
| CCTTATTTCCATGTAAGTAA+TGG | 0.450797 | 6.4:+15155604 | None:intergenic |
| ATTATGACACCAGAGAAATC+AGG | 0.459682 | 6.4:+15155673 | None:intergenic |
| ACGCAACGGCACACATCATA+AGG | 0.467823 | 6.4:+15155872 | None:intergenic |
| AAGAACAAGAGGATTCGATT+TGG | 0.469981 | 6.4:-15155732 | MS.gene85852:CDS |
| AGGCATTGCAAGCGAGCCAA+TGG | 0.488864 | 6.4:+15155815 | None:intergenic |
| AATGGAAGAGAAGTTCTAGC+AGG | 0.508189 | 6.4:+15155622 | None:intergenic |
| GAAGGTTGGTTCAATAAAAG+TGG | 0.529522 | 6.4:+15155931 | None:intergenic |
| CGCTTGCAATGCCTTTGCGG+AGG | 0.535504 | 6.4:-15155806 | MS.gene85852:CDS |
| TTGATGTGCTTAGTGAAGGT+TGG | 0.548850 | 6.4:+15155917 | None:intergenic |
| TTATGACACCAGAGAAATCA+GGG | 0.563321 | 6.4:+15155674 | None:intergenic |
| CAGAAGAATATACTTATGGG+TGG | 0.597191 | 6.4:-15155709 | MS.gene85852:CDS |
| ATGGAAGAGAAGTTCTAGCA+GGG | 0.600945 | 6.4:+15155623 | None:intergenic |
| TGGTAGTGAACAAGAACAAG+AGG | 0.604948 | 6.4:-15155743 | MS.gene85852:CDS |
| ATATTTGATGTGCTTAGTGA+AGG | 0.615139 | 6.4:+15155913 | None:intergenic |
| TATAACAGGCTCCTCCGCAA+AGG | 0.618304 | 6.4:+15155795 | None:intergenic |
| GTCATAATCTATGATTCTGA+TGG | 0.631979 | 6.4:-15155658 | MS.gene85852:CDS |
| AGCACACATGAGCAACGCAA+CGG | 0.634273 | 6.4:+15155858 | None:intergenic |
| AGAAGAATATACTTATGGGT+GGG | 0.641405 | 6.4:-15155708 | MS.gene85852:CDS |
| AATGAAGTTGTGATGATCAG+TGG | 0.678528 | 6.4:-15155763 | MS.gene85852:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGAAGAATATACTTATGGGT+GGG | - | chr6.4:15155838-15155857 | MS.gene85852:CDS | 30.0% | |
| ATATTTGATGTGCTTAGTGA+AGG | + | chr6.4:15155636-15155655 | None:intergenic | 30.0% | |
| GTCATAATCTATGATTCTGA+TGG | - | chr6.4:15155888-15155907 | MS.gene85852:CDS | 30.0% | |
| TCACTAAGCACATCAAATAT+TGG | - | chr6.4:15155636-15155655 | MS.gene85852:CDS | 30.0% | |
| TGGCAGAAGAATATACTTAT+GGG | - | chr6.4:15155834-15155853 | MS.gene85852:CDS | 30.0% | |
| TTGGCAGAAGAATATACTTA+TGG | - | chr6.4:15155833-15155852 | MS.gene85852:CDS | 30.0% | |
| !! | TCATTGTTTAGCTCTATAAC+AGG | + | chr6.4:15155768-15155787 | None:intergenic | 30.0% |
| !!! | ACATCAAATATTGGTTTTGC+TGG | - | chr6.4:15155645-15155664 | MS.gene85852:CDS | 30.0% |
| AAGAACAAGAGGATTCGATT+TGG | - | chr6.4:15155814-15155833 | MS.gene85852:CDS | 35.0% | |
| AATGAAGTTGTGATGATCAG+TGG | - | chr6.4:15155783-15155802 | MS.gene85852:CDS | 35.0% | |
| ATTATGACACCAGAGAAATC+AGG | + | chr6.4:15155876-15155895 | None:intergenic | 35.0% | |
| CAGAAGAATATACTTATGGG+TGG | - | chr6.4:15155837-15155856 | MS.gene85852:CDS | 35.0% | |
| CTTCTCTTCCATTACTTACA+TGG | - | chr6.4:15155934-15155953 | MS.gene85852:CDS | 35.0% | |
| TTATGACACCAGAGAAATCA+GGG | + | chr6.4:15155875-15155894 | None:intergenic | 35.0% | |
| ! | GAAGGTTGGTTCAATAAAAG+TGG | + | chr6.4:15155618-15155637 | None:intergenic | 35.0% |
| TTGATGTGCTTAGTGAAGGT+TGG | + | chr6.4:15155632-15155651 | None:intergenic | 40.0% | |
| ! | AATGGAAGAGAAGTTCTAGC+AGG | + | chr6.4:15155927-15155946 | None:intergenic | 40.0% |
| ! | ATGGAAGAGAAGTTCTAGCA+GGG | + | chr6.4:15155926-15155945 | None:intergenic | 40.0% |
| ! | TGGTAGTGAACAAGAACAAG+AGG | - | chr6.4:15155803-15155822 | MS.gene85852:CDS | 40.0% |
| ACGCAACGGCACACATCATA+AGG | + | chr6.4:15155677-15155696 | None:intergenic | 50.0% | |
| AGCACACATGAGCAACGCAA+CGG | + | chr6.4:15155691-15155710 | None:intergenic | 50.0% | |
| GTGCTTCTCATTCGCGCAAA+TGG | - | chr6.4:15155706-15155725 | MS.gene85852:CDS | 50.0% | |
| TATAACAGGCTCCTCCGCAA+AGG | + | chr6.4:15155754-15155773 | None:intergenic | 50.0% | |
| TGTCAGCTCCCTGATTTCTC+TGG | - | chr6.4:15155864-15155883 | MS.gene85852:CDS | 50.0% | |
| ATTCGCGCAAATGGCGCCAT+TGG | - | chr6.4:15155715-15155734 | MS.gene85852:CDS | 55.0% | |
| GCTCGCTTGCAATGCCTTTG+CGG | - | chr6.4:15155737-15155756 | MS.gene85852:CDS | 55.0% | |
| ! | AGGCATTGCAAGCGAGCCAA+TGG | + | chr6.4:15155734-15155753 | None:intergenic | 55.0% |
| CGCTTGCAATGCCTTTGCGG+AGG | - | chr6.4:15155740-15155759 | MS.gene85852:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 15155606 | 15155962 | 15155606 | ID=MS.gene85852 |
| chr6.4 | mRNA | 15155606 | 15155962 | 15155606 | ID=MS.gene85852.t1;Parent=MS.gene85852 |
| chr6.4 | exon | 15155606 | 15155962 | 15155606 | ID=MS.gene85852.t1.exon1;Parent=MS.gene85852.t1 |
| chr6.4 | CDS | 15155606 | 15155962 | 15155606 | ID=cds.MS.gene85852.t1;Parent=MS.gene85852.t1 |
| Gene Sequence |
| Protein sequence |
