Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058496.t1 | XP_003629835.1 | 96.9 | 163 | 4 | 1 | 1 | 163 | 64 | 225 | 1.50E-87 | 332 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058496.t1 | P32110 | 43.8 | 160 | 86 | 3 | 3 | 160 | 67 | 224 | 7.8e-32 | 138.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058496.t1 | G7L788 | 96.9 | 163 | 4 | 1 | 1 | 163 | 64 | 225 | 1.1e-87 | 332.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050883 | MS.gene058496 | 0.807045 | 6.12E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene058496 | 0.807304 | 5.40E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene058496 | 0.804968 | 1.67E-49 | -1.69E-46 |
| MS.gene055549 | MS.gene058496 | 0.814597 | 1.42E-51 | -1.69E-46 |
| MS.gene055550 | MS.gene058496 | 0.804313 | 2.29E-49 | -1.69E-46 |
| MS.gene056311 | MS.gene058496 | 0.849604 | 2.91E-60 | -1.69E-46 |
| MS.gene057014 | MS.gene058496 | 0.813994 | 1.93E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene058496 | 0.800518 | 1.39E-48 | -1.69E-46 |
| MS.gene058175 | MS.gene058496 | 0.802349 | 5.85E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene058496 | 0.896522 | 3.40E-76 | -1.69E-46 |
| MS.gene058494 | MS.gene058496 | 0.928789 | 1.76E-92 | -1.69E-46 |
| MS.gene058496 | MS.gene058628 | 0.81572 | 8.00E-52 | -1.69E-46 |
| MS.gene058496 | MS.gene058630 | 0.805647 | 1.21E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene060595 | 0.800565 | 1.36E-48 | -1.69E-46 |
| MS.gene058496 | MS.gene061798 | 0.801903 | 7.23E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene062071 | 0.829741 | 4.34E-55 | -1.69E-46 |
| MS.gene058496 | MS.gene068917 | 0.806348 | 8.59E-50 | -1.69E-46 |
| MS.gene058496 | MS.gene071317 | 0.806714 | 7.19E-50 | -1.69E-46 |
| MS.gene058496 | MS.gene071843 | 0.801833 | 7.47E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene072476 | 0.813264 | 2.80E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene073868 | 0.80345 | 3.47E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene21350 | 0.826563 | 2.53E-54 | -1.69E-46 |
| MS.gene058496 | MS.gene21575 | 0.811799 | 5.84E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene21577 | 0.829431 | 5.17E-55 | -1.69E-46 |
| MS.gene058496 | MS.gene24491 | 0.849298 | 3.54E-60 | -1.69E-46 |
| MS.gene058496 | MS.gene27550 | 0.801044 | 1.08E-48 | -1.69E-46 |
| MS.gene058496 | MS.gene27832 | 0.933714 | 1.23E-95 | -1.69E-46 |
| MS.gene058496 | MS.gene27833 | 0.933226 | 2.59E-95 | -1.69E-46 |
| MS.gene058496 | MS.gene27835 | 0.920102 | 1.96E-87 | -1.69E-46 |
| MS.gene058496 | MS.gene27837 | 0.90876 | 1.20E-81 | -1.69E-46 |
| MS.gene058496 | MS.gene29342 | 0.813319 | 2.72E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene29481 | 0.810765 | 9.80E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene31404 | 0.847904 | 8.60E-60 | -1.69E-46 |
| MS.gene058496 | MS.gene38094 | 0.803606 | 3.22E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene38109 | 0.801066 | 1.07E-48 | -1.69E-46 |
| MS.gene058496 | MS.gene38172 | 0.803429 | 3.50E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene38359 | 0.80485 | 1.77E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene38706 | 0.830059 | 3.63E-55 | -1.69E-46 |
| MS.gene058496 | MS.gene40247 | 0.813728 | 2.21E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene40619 | 0.802653 | 5.07E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene40699 | 0.806642 | 7.45E-50 | -1.69E-46 |
| MS.gene058496 | MS.gene41459 | 0.813505 | 2.47E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene44721 | 0.800238 | 1.58E-48 | -1.69E-46 |
| MS.gene058496 | MS.gene44723 | 0.80781 | 4.21E-50 | -1.69E-46 |
| MS.gene058496 | MS.gene46057 | 0.818978 | 1.48E-52 | -1.69E-46 |
| MS.gene058496 | MS.gene46060 | 0.831625 | 1.50E-55 | -1.69E-46 |
| MS.gene058496 | MS.gene46106 | 0.804526 | 2.07E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene47049 | 0.827331 | 1.66E-54 | -1.69E-46 |
| MS.gene058496 | MS.gene49887 | 0.804886 | 1.74E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene51514 | 0.823534 | 1.32E-53 | -1.69E-46 |
| MS.gene058496 | MS.gene53268 | 0.91873 | 1.09E-86 | -1.69E-46 |
| MS.gene058496 | MS.gene53270 | 0.918857 | 9.28E-87 | -1.69E-46 |
| MS.gene058496 | MS.gene54372 | 0.810824 | 9.51E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene54882 | 0.851018 | 1.17E-60 | -1.69E-46 |
| MS.gene058496 | MS.gene55036 | 0.803332 | 3.67E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene58945 | 0.821255 | 4.45E-53 | -1.69E-46 |
| MS.gene058496 | MS.gene60720 | 0.804067 | 2.58E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene60721 | 0.831721 | 1.42E-55 | -1.69E-46 |
| MS.gene058496 | MS.gene60722 | 0.828972 | 6.68E-55 | -1.69E-46 |
| MS.gene058496 | MS.gene64441 | 0.824991 | 5.98E-54 | -1.69E-46 |
| MS.gene058496 | MS.gene67744 | 0.836002 | 1.21E-56 | -1.69E-46 |
| MS.gene058496 | MS.gene69071 | 0.813365 | 2.66E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene73753 | 0.82089 | 5.40E-53 | -1.69E-46 |
| MS.gene058496 | MS.gene74072 | 0.810089 | 1.37E-50 | -1.69E-46 |
| MS.gene058496 | MS.gene76722 | 0.811331 | 7.38E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene77048 | 0.816132 | 6.48E-52 | -1.69E-46 |
| MS.gene058496 | MS.gene78593 | 0.824273 | 8.83E-54 | -1.69E-46 |
| MS.gene058496 | MS.gene80092 | 0.821618 | 3.67E-53 | -1.69E-46 |
| MS.gene058496 | MS.gene85220 | 0.818913 | 1.53E-52 | -1.69E-46 |
| MS.gene058496 | MS.gene85298 | 0.830082 | 3.59E-55 | -1.69E-46 |
| MS.gene058496 | MS.gene85852 | 0.813168 | 2.93E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene88198 | 0.819738 | 9.92E-53 | -1.69E-46 |
| MS.gene058496 | MS.gene88886 | 0.813581 | 2.38E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene88887 | 0.81961 | 1.06E-52 | -1.69E-46 |
| MS.gene058496 | MS.gene88888 | 0.825185 | 5.38E-54 | -1.69E-46 |
| MS.gene058496 | MS.gene89261 | 0.812583 | 3.94E-51 | -1.69E-46 |
| MS.gene058496 | MS.gene91418 | 0.804802 | 1.81E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene91775 | 0.848945 | 4.44E-60 | -1.69E-46 |
| MS.gene058496 | MS.gene92808 | 0.828837 | 7.20E-55 | -1.69E-46 |
| MS.gene058496 | MS.gene93015 | 0.801561 | 8.50E-49 | -1.69E-46 |
| MS.gene058496 | MS.gene94263 | 0.815394 | 9.46E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058496.t1 | MTR_8g087410 | 96.933 | 163 | 4 | 1 | 1 | 163 | 64 | 225 | 5.00e-113 | 320 |
| MS.gene058496.t1 | MTR_8g087425 | 60.494 | 162 | 63 | 1 | 1 | 162 | 63 | 223 | 1.18e-59 | 185 |
| MS.gene058496.t1 | MTR_6g080440 | 51.333 | 150 | 70 | 2 | 1 | 149 | 61 | 208 | 1.29e-47 | 154 |
| MS.gene058496.t1 | MTR_4g019780 | 49.660 | 147 | 73 | 1 | 3 | 149 | 68 | 213 | 6.43e-41 | 137 |
| MS.gene058496.t1 | MTR_7g065720 | 47.020 | 151 | 76 | 3 | 1 | 149 | 65 | 213 | 3.01e-40 | 135 |
| MS.gene058496.t1 | MTR_7g065590 | 42.857 | 154 | 85 | 3 | 1 | 153 | 65 | 216 | 4.82e-37 | 127 |
| MS.gene058496.t1 | MTR_7g065600 | 43.506 | 154 | 84 | 3 | 1 | 153 | 65 | 216 | 6.60e-37 | 127 |
| MS.gene058496.t1 | MTR_7g065630 | 40.373 | 161 | 89 | 4 | 3 | 162 | 85 | 239 | 7.81e-37 | 127 |
| MS.gene058496.t1 | MTR_7g065660 | 40.909 | 154 | 88 | 3 | 1 | 153 | 65 | 216 | 8.59e-37 | 126 |
| MS.gene058496.t1 | MTR_7g065290 | 44.156 | 154 | 83 | 3 | 1 | 153 | 65 | 216 | 2.01e-36 | 125 |
| MS.gene058496.t1 | MTR_7g065740 | 41.722 | 151 | 84 | 3 | 1 | 149 | 65 | 213 | 4.74e-36 | 124 |
| MS.gene058496.t1 | MTR_3g099757 | 44.218 | 147 | 81 | 1 | 1 | 147 | 63 | 208 | 4.23e-35 | 122 |
| MS.gene058496.t1 | MTR_1g090150 | 41.509 | 159 | 89 | 3 | 1 | 157 | 61 | 217 | 7.42e-35 | 121 |
| MS.gene058496.t1 | MTR_7g065750 | 39.634 | 164 | 95 | 3 | 1 | 162 | 65 | 226 | 5.62e-34 | 119 |
| MS.gene058496.t1 | MTR_4g019790 | 39.264 | 163 | 96 | 3 | 1 | 162 | 65 | 225 | 1.01e-33 | 119 |
| MS.gene058496.t1 | MTR_7g065230 | 39.752 | 161 | 89 | 4 | 1 | 160 | 65 | 218 | 1.26e-33 | 118 |
| MS.gene058496.t1 | MTR_7g065270 | 41.139 | 158 | 90 | 3 | 1 | 157 | 65 | 220 | 6.10e-33 | 116 |
| MS.gene058496.t1 | MTR_7g065265 | 42.138 | 159 | 87 | 5 | 1 | 157 | 65 | 220 | 7.81e-33 | 116 |
| MS.gene058496.t1 | MTR_7g065710 | 40.667 | 150 | 82 | 4 | 1 | 149 | 62 | 205 | 1.60e-32 | 115 |
| MS.gene058496.t1 | MTR_7g065680 | 35.404 | 161 | 97 | 4 | 3 | 162 | 67 | 221 | 5.24e-31 | 111 |
| MS.gene058496.t1 | MTR_5g040430 | 39.216 | 153 | 86 | 3 | 1 | 147 | 65 | 216 | 6.22e-31 | 111 |
| MS.gene058496.t1 | MTR_1g115195 | 40.000 | 160 | 92 | 3 | 1 | 157 | 61 | 219 | 9.26e-31 | 111 |
| MS.gene058496.t1 | MTR_7g065260 | 42.593 | 162 | 80 | 5 | 1 | 156 | 72 | 226 | 3.55e-30 | 109 |
| MS.gene058496.t1 | MTR_1g090060 | 37.654 | 162 | 98 | 2 | 1 | 160 | 61 | 221 | 1.41e-29 | 108 |
| MS.gene058496.t1 | MTR_1g090070 | 39.869 | 153 | 88 | 3 | 1 | 150 | 67 | 218 | 4.82e-29 | 107 |
| MS.gene058496.t1 | MTR_4g059730 | 38.788 | 165 | 84 | 4 | 1 | 156 | 62 | 218 | 1.88e-28 | 105 |
| MS.gene058496.t1 | MTR_2g070200 | 37.975 | 158 | 94 | 3 | 1 | 156 | 62 | 217 | 6.56e-28 | 103 |
| MS.gene058496.t1 | MTR_2g070140 | 42.177 | 147 | 81 | 3 | 1 | 146 | 46 | 189 | 2.19e-27 | 102 |
| MS.gene058496.t1 | MTR_7g065700 | 41.096 | 146 | 79 | 4 | 1 | 145 | 65 | 204 | 4.83e-27 | 101 |
| MS.gene058496.t1 | MTR_2g070150 | 42.466 | 146 | 80 | 3 | 1 | 145 | 62 | 204 | 6.92e-27 | 100 |
| MS.gene058496.t1 | MTR_2g070120 | 39.024 | 164 | 92 | 4 | 1 | 163 | 62 | 218 | 1.80e-26 | 100 |
| MS.gene058496.t1 | MTR_7g065640 | 35.714 | 154 | 78 | 3 | 1 | 153 | 65 | 198 | 2.69e-26 | 99.4 |
| MS.gene058496.t1 | MTR_8g061950 | 39.216 | 153 | 86 | 4 | 1 | 147 | 65 | 216 | 6.64e-25 | 96.3 |
| MS.gene058496.t1 | MTR_1g090100 | 37.748 | 151 | 91 | 2 | 1 | 149 | 60 | 209 | 1.93e-24 | 94.7 |
| MS.gene058496.t1 | MTR_1g090090 | 35.443 | 158 | 99 | 2 | 1 | 156 | 61 | 217 | 7.93e-24 | 93.2 |
| MS.gene058496.t1 | MTR_2g070180 | 39.456 | 147 | 85 | 3 | 1 | 146 | 62 | 205 | 9.82e-24 | 92.8 |
| MS.gene058496.t1 | MTR_5g037380 | 36.076 | 158 | 92 | 4 | 1 | 150 | 63 | 219 | 1.45e-23 | 92.8 |
| MS.gene058496.t1 | MTR_3g467420 | 42.063 | 126 | 70 | 2 | 1 | 125 | 67 | 190 | 4.93e-23 | 91.3 |
| MS.gene058496.t1 | MTR_2g070130 | 36.735 | 147 | 89 | 3 | 1 | 146 | 62 | 205 | 6.24e-23 | 90.9 |
| MS.gene058496.t1 | MTR_2g070210 | 40.945 | 127 | 70 | 4 | 1 | 125 | 64 | 187 | 1.27e-22 | 90.1 |
| MS.gene058496.t1 | MTR_2g070060 | 38.583 | 127 | 73 | 4 | 1 | 125 | 62 | 185 | 5.49e-22 | 88.2 |
| MS.gene058496.t1 | MTR_8g056940 | 38.760 | 129 | 76 | 2 | 1 | 128 | 62 | 188 | 4.52e-21 | 85.9 |
| MS.gene058496.t1 | MTR_4g124130 | 41.270 | 126 | 71 | 2 | 1 | 125 | 63 | 186 | 9.96e-21 | 85.1 |
| MS.gene058496.t1 | MTR_3g467430 | 40.476 | 126 | 72 | 2 | 1 | 125 | 67 | 190 | 1.36e-19 | 82.0 |
| MS.gene058496.t1 | MTR_5g076900 | 35.200 | 125 | 79 | 1 | 1 | 125 | 65 | 187 | 3.29e-19 | 81.3 |
| MS.gene058496.t1 | MTR_2g070110 | 37.302 | 126 | 75 | 3 | 1 | 125 | 62 | 184 | 1.29e-16 | 74.3 |
| MS.gene058496.t1 | MTR_2g070070 | 36.424 | 151 | 88 | 6 | 1 | 149 | 62 | 206 | 2.16e-16 | 73.6 |
| MS.gene058496.t1 | MTR_0186s0030 | 48.000 | 75 | 38 | 1 | 1 | 74 | 62 | 136 | 1.05e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058496.t1 | AT2G29420 | 38.608 | 158 | 96 | 1 | 1 | 158 | 67 | 223 | 4.50e-37 | 127 |
| MS.gene058496.t1 | AT2G29460 | 40.741 | 162 | 92 | 3 | 1 | 160 | 65 | 224 | 7.15e-35 | 121 |
| MS.gene058496.t1 | AT2G29450 | 42.208 | 154 | 84 | 4 | 1 | 151 | 64 | 215 | 4.10e-34 | 119 |
| MS.gene058496.t1 | AT3G09270 | 43.243 | 148 | 82 | 2 | 1 | 147 | 65 | 211 | 1.18e-33 | 118 |
| MS.gene058496.t1 | AT2G29490 | 41.611 | 149 | 83 | 3 | 1 | 147 | 65 | 211 | 1.96e-32 | 115 |
| MS.gene058496.t1 | AT2G29480 | 38.272 | 162 | 96 | 3 | 1 | 160 | 65 | 224 | 2.09e-32 | 115 |
| MS.gene058496.t1 | AT2G29470 | 40.268 | 149 | 85 | 3 | 1 | 147 | 66 | 212 | 1.80e-31 | 113 |
| MS.gene058496.t1 | AT2G29440 | 38.889 | 162 | 95 | 3 | 1 | 160 | 64 | 223 | 4.60e-31 | 112 |
| MS.gene058496.t1 | AT1G10370 | 40.881 | 159 | 86 | 4 | 1 | 152 | 63 | 220 | 5.75e-27 | 101 |
| MS.gene058496.t1 | AT1G17170 | 38.217 | 157 | 92 | 4 | 1 | 156 | 62 | 214 | 1.85e-26 | 100 |
| MS.gene058496.t1 | AT1G17180 | 38.255 | 149 | 88 | 3 | 3 | 150 | 64 | 209 | 8.17e-26 | 98.6 |
| MS.gene058496.t1 | AT1G59670 | 38.562 | 153 | 86 | 4 | 1 | 146 | 65 | 216 | 1.57e-25 | 97.8 |
| MS.gene058496.t1 | AT1G78370 | 33.775 | 151 | 96 | 3 | 1 | 150 | 62 | 209 | 6.24e-24 | 93.6 |
| MS.gene058496.t1 | AT1G17190 | 36.646 | 161 | 98 | 3 | 1 | 160 | 63 | 220 | 6.79e-24 | 93.2 |
| MS.gene058496.t1 | AT1G59700 | 38.732 | 142 | 80 | 3 | 1 | 135 | 65 | 206 | 1.07e-23 | 93.2 |
| MS.gene058496.t1 | AT3G43800 | 40.411 | 146 | 81 | 3 | 1 | 142 | 64 | 207 | 1.01e-22 | 90.5 |
| MS.gene058496.t1 | AT1G78380 | 36.879 | 141 | 76 | 3 | 1 | 140 | 62 | 190 | 3.18e-22 | 89.0 |
| MS.gene058496.t1 | AT1G78340 | 39.200 | 125 | 73 | 3 | 1 | 124 | 62 | 184 | 1.25e-21 | 87.4 |
| MS.gene058496.t1 | AT1G78360 | 34.591 | 159 | 100 | 4 | 1 | 158 | 63 | 218 | 1.62e-21 | 87.0 |
| MS.gene058496.t1 | AT1G27130 | 32.500 | 160 | 101 | 4 | 2 | 155 | 67 | 225 | 2.30e-21 | 87.0 |
| MS.gene058496.t1 | AT1G78320 | 34.395 | 157 | 97 | 4 | 1 | 154 | 62 | 215 | 4.62e-21 | 85.9 |
| MS.gene058496.t1 | AT1G10360 | 37.324 | 142 | 82 | 3 | 1 | 135 | 63 | 204 | 7.03e-21 | 85.5 |
| MS.gene058496.t1 | AT1G69930 | 30.435 | 161 | 105 | 4 | 1 | 155 | 70 | 229 | 8.44e-21 | 85.5 |
| MS.gene058496.t1 | AT1G53680 | 40.476 | 126 | 70 | 3 | 3 | 125 | 67 | 190 | 1.56e-20 | 84.7 |
| MS.gene058496.t1 | AT1G74590 | 30.573 | 157 | 103 | 3 | 1 | 152 | 65 | 220 | 9.05e-20 | 82.8 |
| MS.gene058496.t1 | AT5G62480 | 32.934 | 167 | 101 | 4 | 1 | 158 | 66 | 230 | 2.64e-19 | 81.6 |
| MS.gene058496.t1 | AT1G27140 | 31.677 | 161 | 103 | 4 | 2 | 156 | 68 | 227 | 5.19e-19 | 80.9 |
| MS.gene058496.t1 | AT1G69920 | 31.875 | 160 | 98 | 5 | 2 | 153 | 95 | 251 | 2.94e-18 | 79.3 |
| MS.gene058496.t1 | AT1G78320 | 31.111 | 135 | 88 | 3 | 22 | 154 | 57 | 188 | 5.91e-14 | 66.6 |
Find 47 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCAATTTGGGAAGAAAT+TGG | 0.111299 | 4.1:+13288653 | MS.gene058496:CDS |
| AAAGTTAGAAGAGGAGATAA+AGG | 0.273945 | 4.1:+13288562 | MS.gene058496:CDS |
| GACCCAATTTCTTCCCAAAT+TGG | 0.274164 | 4.1:-13288656 | None:intergenic |
| ATAAAGGGAAAGAAATTCTT+TGG | 0.305857 | 4.1:+13288578 | MS.gene058496:CDS |
| AAAGATCTCAGGCTCGCTTT+TGG | 0.326368 | 4.1:+13287613 | MS.gene058496:CDS |
| TATAGCTTGCGCTGGGTTCA+TGG | 0.334296 | 4.1:+13288474 | MS.gene058496:intron |
| AATGTCCTTCCATCTCTTCA+TGG | 0.338522 | 4.1:+13288702 | MS.gene058496:CDS |
| TTCCAATTTGGGAAGAAATT+GGG | 0.398944 | 4.1:+13288654 | MS.gene058496:CDS |
| TTATTGCCACCGTCGCATAA+AGG | 0.420018 | 4.1:+13288793 | MS.gene058496:CDS |
| ACCTTGATCTTGCTCTTGGA+TGG | 0.423990 | 4.1:+13288618 | MS.gene058496:CDS |
| TAAAGAATCATTTCATCCCT+TGG | 0.429286 | 4.1:-13288773 | None:intergenic |
| TGGATTGCATTGATTAAGAA+AGG | 0.435401 | 4.1:+13288494 | MS.gene058496:CDS |
| GGCTAAGTTATCTGATGAAA+AGG | 0.437582 | 4.1:+13287635 | MS.gene058496:CDS |
| GGGTACCTTGATCTTGCTCT+TGG | 0.453072 | 4.1:+13288614 | MS.gene058496:CDS |
| TCTTTGGAGGGGACACTATC+GGG | 0.454808 | 4.1:+13288594 | MS.gene058496:CDS |
| TAAATTATTATAGCTTGCGC+TGG | 0.466026 | 4.1:+13288466 | MS.gene058496:intron |
| TGATTAAGAAAGGTGATGAA+TGG | 0.474122 | 4.1:+13288504 | MS.gene058496:CDS |
| AAAGGAGGCAAGAGAAATCA+TGG | 0.475218 | 4.1:+13288538 | MS.gene058496:CDS |
| TTCTTTGGAGGGGACACTAT+CGG | 0.475345 | 4.1:+13288593 | MS.gene058496:CDS |
| GATTAAGAAAGGTGATGAAT+GGG | 0.480475 | 4.1:+13288505 | MS.gene058496:CDS |
| AAGTTAGAAGAGGAGATAAA+GGG | 0.491500 | 4.1:+13288563 | MS.gene058496:CDS |
| GAAAGGTGATGAATGGGAAA+AGG | 0.495659 | 4.1:+13288511 | MS.gene058496:CDS |
| TGAGATCTTTGATAAGGATC+AGG | 0.498359 | 4.1:-13287600 | None:intergenic |
| TGATCCTTATCAAAGATCTC+AGG | 0.520135 | 4.1:+13287602 | MS.gene058496:CDS |
| CGAGCCTGAGATCTTTGATA+AGG | 0.520914 | 4.1:-13287606 | None:intergenic |
| AAACACTCCTTGATGATGAT+AGG | 0.524604 | 4.1:-13288746 | None:intergenic |
| AATCATGGAAAAGTTAGAAG+AGG | 0.531445 | 4.1:+13288553 | MS.gene058496:CDS |
| AGGGAAAGAAATTCTTTGGA+GGG | 0.536580 | 4.1:+13288582 | MS.gene058496:CDS |
| ATTGATCTTCCATGAAGAGA+TGG | 0.543546 | 4.1:-13288711 | None:intergenic |
| TGAATGGGAAAAGGCTCTAA+AGG | 0.545680 | 4.1:+13288520 | MS.gene058496:CDS |
| TCCATCCAAGAGCAAGATCA+AGG | 0.557485 | 4.1:-13288619 | None:intergenic |
| TAAGGATCAGGAGGCAGCAA+TGG | 0.562517 | 4.1:-13287588 | None:intergenic |
| CAAGGAGTGTTTGCCACCAA+GGG | 0.564625 | 4.1:+13288757 | MS.gene058496:CDS |
| TCAAGGAGTGTTTGCCACCA+AGG | 0.571195 | 4.1:+13288756 | MS.gene058496:CDS |
| ACTCCTTGATGATGATAGGA+TGG | 0.572678 | 4.1:-13288742 | None:intergenic |
| TTGAATACATTGATGAAACA+TGG | 0.575829 | 4.1:+13287556 | MS.gene058496:CDS |
| AAGGGAAAGAAATTCTTTGG+AGG | 0.576725 | 4.1:+13288581 | MS.gene058496:CDS |
| GAGCCATCCTATCATCATCA+AGG | 0.581782 | 4.1:+13288739 | MS.gene058496:CDS |
| AAATTATTATAGCTTGCGCT+GGG | 0.589664 | 4.1:+13288467 | MS.gene058496:intron |
| AAGAATACTCCTTTATGCGA+CGG | 0.595491 | 4.1:-13288802 | None:intergenic |
| GGGAAAGAAATTCTTTGGAG+GGG | 0.609810 | 4.1:+13288583 | MS.gene058496:CDS |
| ATCTTCCATGAAGAGATGGA+AGG | 0.621011 | 4.1:-13288707 | None:intergenic |
| AGAATCATTTCATCCCTTGG+TGG | 0.643379 | 4.1:-13288770 | None:intergenic |
| GATCTTTGATAAGGATCAGG+AGG | 0.658191 | 4.1:-13287597 | None:intergenic |
| ATGGGAAAAGGCTCTAAAGG+AGG | 0.677413 | 4.1:+13288523 | MS.gene058496:CDS |
| AATACTCCTTTATGCGACGG+TGG | 0.688837 | 4.1:-13288799 | None:intergenic |
| GAGTATTCTTCAACTCATCG+TGG | 0.696267 | 4.1:+13288815 | MS.gene058496:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAATATAAAACTATTTTAA+AGG | - | chr4.1:13288244-13288263 | None:intergenic | 10.0% |
| !! | ATAATTTACACATACAAAAT+AGG | - | chr4.1:13288454-13288473 | None:intergenic | 15.0% |
| !!! | AATAATTGTTTTATGAACTT+AGG | + | chr4.1:13287851-13287870 | MS.gene058496:intron | 15.0% |
| !! | TATCTCTTGAATTTATTAGA+TGG | - | chr4.1:13288035-13288054 | None:intergenic | 20.0% |
| !! | TATGTTGTTCAAATTTAACT+AGG | + | chr4.1:13287681-13287700 | MS.gene058496:intron | 20.0% |
| !!! | AAAATTATGTTTCCTTTTTG+AGG | + | chr4.1:13287750-13287769 | MS.gene058496:intron | 20.0% |
| ! | AAAATATTCTTGCCTCAAAA+AGG | - | chr4.1:13287765-13287784 | None:intergenic | 25.0% |
| ! | AAATACTACGTTTCTTTCAA+AGG | + | chr4.1:13287721-13287740 | MS.gene058496:intron | 25.0% |
| ! | ATAAAGGGAAAGAAATTCTT+TGG | + | chr4.1:13288578-13288597 | MS.gene058496:CDS | 25.0% |
| ! | ATTACTTGAGTACAATTGTT+TGG | + | chr4.1:13287906-13287925 | MS.gene058496:intron | 25.0% |
| ! | TTGAATACATTGATGAAACA+TGG | + | chr4.1:13287556-13287575 | MS.gene058496:CDS | 25.0% |
| !!! | TTGAGGCAAGAATATTTTTT+TGG | + | chr4.1:13287767-13287786 | MS.gene058496:intron | 25.0% |
| !!! | TTTTTTTGGTGTTAATCATC+CGG | + | chr4.1:13287781-13287800 | MS.gene058496:intron | 25.0% |
| AAAGTTAGAAGAGGAGATAA+AGG | + | chr4.1:13288562-13288581 | MS.gene058496:CDS | 30.0% | |
| AAGTTAGAAGAGGAGATAAA+GGG | + | chr4.1:13288563-13288582 | MS.gene058496:CDS | 30.0% | |
| AATCATGGAAAAGTTAGAAG+AGG | + | chr4.1:13288553-13288572 | MS.gene058496:CDS | 30.0% | |
| ACCGTTTCTATCAACATATT+AGG | - | chr4.1:13288303-13288322 | None:intergenic | 30.0% | |
| ATTTAACTAGGCTTATTCTG+AGG | + | chr4.1:13287693-13287712 | MS.gene058496:intron | 30.0% | |
| CATGATCAATATGTGTTTAG+TGG | + | chr4.1:13287878-13287897 | MS.gene058496:intron | 30.0% | |
| GATGTTAATTATTCCATTCC+AGG | + | chr4.1:13288124-13288143 | MS.gene058496:intron | 30.0% | |
| GATTAAGAAAGGTGATGAAT+GGG | + | chr4.1:13288505-13288524 | MS.gene058496:CDS | 30.0% | |
| TAAAGAATCATTTCATCCCT+TGG | - | chr4.1:13288776-13288795 | None:intergenic | 30.0% | |
| TGATTAAGAAAGGTGATGAA+TGG | + | chr4.1:13288504-13288523 | MS.gene058496:CDS | 30.0% | |
| TGTTAATCATCCGGTTAATA+GGG | + | chr4.1:13287790-13287809 | MS.gene058496:intron | 30.0% | |
| TTCCAATTTGGGAAGAAATT+GGG | + | chr4.1:13288654-13288673 | MS.gene058496:CDS | 30.0% | |
| ! | AAATTATTATAGCTTGCGCT+GGG | + | chr4.1:13288467-13288486 | MS.gene058496:intron | 30.0% |
| ! | GCCTAATATGTTGATAGAAA+CGG | + | chr4.1:13288299-13288318 | MS.gene058496:intron | 30.0% |
| ! | TAAATTATTATAGCTTGCGC+TGG | + | chr4.1:13288466-13288485 | MS.gene058496:intron | 30.0% |
| !! | AGATGGAAGGACATTTTAAT+GGG | - | chr4.1:13288697-13288716 | None:intergenic | 30.0% |
| !! | CACTTGTTTTCTTCCAATTT+GGG | + | chr4.1:13288643-13288662 | MS.gene058496:CDS | 30.0% |
| !! | TCACTTGTTTTCTTCCAATT+TGG | + | chr4.1:13288642-13288661 | MS.gene058496:CDS | 30.0% |
| !! | TGGATTGCATTGATTAAGAA+AGG | + | chr4.1:13288494-13288513 | MS.gene058496:CDS | 30.0% |
| AAGAATACTCCTTTATGCGA+CGG | - | chr4.1:13288805-13288824 | None:intergenic | 35.0% | |
| AAGGGAAAGAAATTCTTTGG+AGG | + | chr4.1:13288581-13288600 | MS.gene058496:CDS | 35.0% | |
| ACAATACTTGAATGTATGCC+TGG | - | chr4.1:13288145-13288164 | None:intergenic | 35.0% | |
| AGGGAAAGAAATTCTTTGGA+GGG | + | chr4.1:13288582-13288601 | MS.gene058496:CDS | 35.0% | |
| ATTGATCTTCCATGAAGAGA+TGG | - | chr4.1:13288714-13288733 | None:intergenic | 35.0% | |
| CTTCCAATTTGGGAAGAAAT+TGG | + | chr4.1:13288653-13288672 | MS.gene058496:CDS | 35.0% | |
| GGCTAAGTTATCTGATGAAA+AGG | + | chr4.1:13287635-13287654 | MS.gene058496:CDS | 35.0% | |
| GTGTTAATCATCCGGTTAAT+AGG | + | chr4.1:13287789-13287808 | MS.gene058496:intron | 35.0% | |
| GTTAATCATCCGGTTAATAG+GGG | + | chr4.1:13287791-13287810 | MS.gene058496:intron | 35.0% | |
| TGAGATCTTTGATAAGGATC+AGG | - | chr4.1:13287603-13287622 | None:intergenic | 35.0% | |
| TGATCCTTATCAAAGATCTC+AGG | + | chr4.1:13287602-13287621 | MS.gene058496:CDS | 35.0% | |
| ! | GAGATGGAAGGACATTTTAA+TGG | - | chr4.1:13288698-13288717 | None:intergenic | 35.0% |
| !! | AAACACTCCTTGATGATGAT+AGG | - | chr4.1:13288749-13288768 | None:intergenic | 35.0% |
| AAAGGAGGCAAGAGAAATCA+TGG | + | chr4.1:13288538-13288557 | MS.gene058496:CDS | 40.0% | |
| AATGTCCTTCCATCTCTTCA+TGG | + | chr4.1:13288702-13288721 | MS.gene058496:CDS | 40.0% | |
| ACTTGAATGTATGCCTGGAA+TGG | - | chr4.1:13288140-13288159 | None:intergenic | 40.0% | |
| AGAATCATTTCATCCCTTGG+TGG | - | chr4.1:13288773-13288792 | None:intergenic | 40.0% | |
| ATCTTCCATGAAGAGATGGA+AGG | - | chr4.1:13288710-13288729 | None:intergenic | 40.0% | |
| GAAAGGTGATGAATGGGAAA+AGG | + | chr4.1:13288511-13288530 | MS.gene058496:CDS | 40.0% | |
| GACCCAATTTCTTCCCAAAT+TGG | - | chr4.1:13288659-13288678 | None:intergenic | 40.0% | |
| GAGTATTCTTCAACTCATCG+TGG | + | chr4.1:13288815-13288834 | MS.gene058496:CDS | 40.0% | |
| GATCTTTGATAAGGATCAGG+AGG | - | chr4.1:13287600-13287619 | None:intergenic | 40.0% | |
| ! | GGGAAAGAAATTCTTTGGAG+GGG | + | chr4.1:13288583-13288602 | MS.gene058496:CDS | 40.0% |
| ! | TGAATGGGAAAAGGCTCTAA+AGG | + | chr4.1:13288520-13288539 | MS.gene058496:CDS | 40.0% |
| !! | ACTCCTTGATGATGATAGGA+TGG | - | chr4.1:13288745-13288764 | None:intergenic | 40.0% |
| AATACTCCTTTATGCGACGG+TGG | - | chr4.1:13288802-13288821 | None:intergenic | 45.0% | |
| ACCTTGATCTTGCTCTTGGA+TGG | + | chr4.1:13288618-13288637 | MS.gene058496:CDS | 45.0% | |
| ACTCTCCTTCCCCTATTAAC+CGG | - | chr4.1:13287803-13287822 | None:intergenic | 45.0% | |
| ATCATCCGGTTAATAGGGGA+AGG | + | chr4.1:13287795-13287814 | MS.gene058496:intron | 45.0% | |
| CGAGCCTGAGATCTTTGATA+AGG | - | chr4.1:13287609-13287628 | None:intergenic | 45.0% | |
| GAGCCATCCTATCATCATCA+AGG | + | chr4.1:13288739-13288758 | MS.gene058496:CDS | 45.0% | |
| TCCATCCAAGAGCAAGATCA+AGG | - | chr4.1:13288622-13288641 | None:intergenic | 45.0% | |
| TTATTGCCACCGTCGCATAA+AGG | + | chr4.1:13288793-13288812 | MS.gene058496:CDS | 45.0% | |
| ! | AAAGATCTCAGGCTCGCTTT+TGG | + | chr4.1:13287613-13287632 | MS.gene058496:CDS | 45.0% |
| ! | TTCTTTGGAGGGGACACTAT+CGG | + | chr4.1:13288593-13288612 | MS.gene058496:CDS | 45.0% |
| !! | AAGATCTCAGGCTCGCTTTT+GGG | + | chr4.1:13287614-13287633 | MS.gene058496:CDS | 45.0% |
| !! | ATGGGAAAAGGCTCTAAAGG+AGG | + | chr4.1:13288523-13288542 | MS.gene058496:CDS | 45.0% |
| GGAAGGAGAGTTCTCTCGAA+AGG | + | chr4.1:13287812-13287831 | MS.gene058496:intron | 50.0% | |
| GGGTACCTTGATCTTGCTCT+TGG | + | chr4.1:13288614-13288633 | MS.gene058496:CDS | 50.0% | |
| TAAGGATCAGGAGGCAGCAA+TGG | - | chr4.1:13287591-13287610 | None:intergenic | 50.0% | |
| ! | TATAGCTTGCGCTGGGTTCA+TGG | + | chr4.1:13288474-13288493 | MS.gene058496:intron | 50.0% |
| ! | TCTTTGGAGGGGACACTATC+GGG | + | chr4.1:13288594-13288613 | MS.gene058496:CDS | 50.0% |
| !! | CAAGGAGTGTTTGCCACCAA+GGG | + | chr4.1:13288757-13288776 | MS.gene058496:CDS | 50.0% |
| !! | TCAAGGAGTGTTTGCCACCA+AGG | + | chr4.1:13288756-13288775 | MS.gene058496:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 13287528 | 13288841 | 13287528 | ID=MS.gene058496 |
| chr4.1 | mRNA | 13287528 | 13288841 | 13287528 | ID=MS.gene058496.t1;Parent=MS.gene058496 |
| chr4.1 | exon | 13287528 | 13287656 | 13287528 | ID=MS.gene058496.t1.exon1;Parent=MS.gene058496.t1 |
| chr4.1 | CDS | 13287528 | 13287656 | 13287528 | ID=cds.MS.gene058496.t1;Parent=MS.gene058496.t1 |
| chr4.1 | exon | 13288479 | 13288841 | 13288479 | ID=MS.gene058496.t1.exon2;Parent=MS.gene058496.t1 |
| chr4.1 | CDS | 13288479 | 13288841 | 13288479 | ID=cds.MS.gene058496.t1;Parent=MS.gene058496.t1 |
| Gene Sequence |
| Protein sequence |
