Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86229.t1 | XP_003613104.1 | 98.8 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 5.60E-35 | 156.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86229.t1 | G7JXS7 | 98.8 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 4.1e-35 | 156.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050326 | MS.gene86229 | 0.801091 | 1.06E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86229.t1 | MTR_5g032790 | 98.750 | 80 | 1 | 0 | 1 | 80 | 1 | 80 | 1.01e-54 | 164 |
| MS.gene86229.t1 | MTR_8g069350 | 52.577 | 97 | 25 | 2 | 1 | 76 | 6 | 102 | 8.73e-28 | 96.7 |
| MS.gene86229.t1 | MTR_3g461010 | 50.000 | 54 | 27 | 0 | 24 | 77 | 49 | 102 | 4.79e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86229.t1 | AT1G67910 | 61.290 | 62 | 21 | 2 | 6 | 64 | 14 | 75 | 1.32e-18 | 73.2 |
| MS.gene86229.t1 | AT1G67910 | 61.290 | 62 | 21 | 2 | 6 | 64 | 14 | 75 | 1.32e-18 | 73.2 |
| MS.gene86229.t1 | AT1G24577 | 67.442 | 43 | 14 | 0 | 20 | 62 | 19 | 61 | 2.69e-16 | 67.0 |
Find 13 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACACATGCTGGATCATTT+AGG | 0.312387 | 5.1:-23340890 | MS.gene86229:CDS |
| ACAAAAGATCTAATCCTTGT+TGG | 0.397439 | 5.1:+23340773 | None:intergenic |
| GGTTCTGTGGTTGATGCTAA+TGG | 0.469525 | 5.1:-23340801 | MS.gene86229:CDS |
| AATGATCCAGCATGTGTTGT+TGG | 0.473199 | 5.1:+23340894 | None:intergenic |
| TGCTCTCCAACAACACATGC+TGG | 0.498861 | 5.1:-23340900 | MS.gene86229:CDS |
| ATCGTACACCTAGTCTCATA+AGG | 0.501030 | 5.1:-23340857 | MS.gene86229:CDS |
| ATCATCTACTCATGGTTCTG+TGG | 0.546523 | 5.1:-23340814 | MS.gene86229:CDS |
| ATGCTGGATCATTTAGGTGT+AGG | 0.574303 | 5.1:-23340884 | MS.gene86229:CDS |
| GAGTCCTCATCATCTACTCA+TGG | 0.578601 | 5.1:-23340822 | MS.gene86229:CDS |
| TGCTAATGGTGATGCCAACA+AGG | 0.638353 | 5.1:-23340787 | MS.gene86229:CDS |
| AGAACCATGAGTAGATGATG+AGG | 0.647029 | 5.1:+23340818 | None:intergenic |
| AGAACTGATGTGATTCAACA+AGG | 0.659448 | 5.1:-23340966 | MS.gene86229:CDS |
| ACTGATGTGATTCAACAAGG+TGG | 0.724853 | 5.1:-23340963 | MS.gene86229:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTCATAAGGACAAAAAGTA+TGG | - | chr5.1:23340940-23340959 | MS.gene86229:CDS | 30.0% | |
| AGAACTGATGTGATTCAACA+AGG | - | chr5.1:23340818-23340837 | MS.gene86229:CDS | 35.0% | |
| ! | CTTTTTGTCCTTATGAGACT+AGG | + | chr5.1:23340938-23340957 | None:intergenic | 35.0% |
| AATGATCCAGCATGTGTTGT+TGG | + | chr5.1:23340893-23340912 | None:intergenic | 40.0% | |
| ACTGATGTGATTCAACAAGG+TGG | - | chr5.1:23340821-23340840 | MS.gene86229:CDS | 40.0% | |
| AGAACCATGAGTAGATGATG+AGG | + | chr5.1:23340969-23340988 | None:intergenic | 40.0% | |
| ATCGTACACCTAGTCTCATA+AGG | - | chr5.1:23340927-23340946 | MS.gene86229:CDS | 40.0% | |
| ATGCTGGATCATTTAGGTGT+AGG | - | chr5.1:23340900-23340919 | MS.gene86229:CDS | 40.0% | |
| CAACACATGCTGGATCATTT+AGG | - | chr5.1:23340894-23340913 | MS.gene86229:CDS | 40.0% | |
| ! | ATCATCTACTCATGGTTCTG+TGG | - | chr5.1:23340970-23340989 | MS.gene86229:CDS | 40.0% |
| GAGTCCTCATCATCTACTCA+TGG | - | chr5.1:23340962-23340981 | MS.gene86229:CDS | 45.0% | |
| ! | GGTTCTGTGGTTGATGCTAA+TGG | - | chr5.1:23340983-23341002 | MS.gene86229:CDS | 45.0% |
| ! | TGCTAATGGTGATGCCAACA+AGG | - | chr5.1:23340997-23341016 | MS.gene86229:CDS | 45.0% |
| !!! | ACAAGGTGGTCTTTTGAGAC+AGG | - | chr5.1:23340835-23340854 | MS.gene86229:CDS | 45.0% |
| TGCTCTCCAACAACACATGC+TGG | - | chr5.1:23340884-23340903 | MS.gene86229:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 23340782 | 23341024 | 23340782 | ID=MS.gene86229 |
| chr5.1 | mRNA | 23340782 | 23341024 | 23340782 | ID=MS.gene86229.t1;Parent=MS.gene86229 |
| chr5.1 | exon | 23340782 | 23341024 | 23340782 | ID=MS.gene86229.t1.exon1;Parent=MS.gene86229.t1 |
| chr5.1 | CDS | 23340782 | 23341024 | 23340782 | ID=cds.MS.gene86229.t1;Parent=MS.gene86229.t1 |
| Gene Sequence |
| Protein sequence |
