AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86281

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86281.t1 MTR_1g009660 86.094 489 56 3 1 484 1 482 0.0 667
MS.gene86281.t1 MTR_3g108830 74.468 47 11 1 439 484 446 492 8.77e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 82 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAATGATCTTCTTCTTAA+TGG 0.099182 1.3:-10539243 None:intergenic
CAACAACAATTAGCAGTAAA+AGG 0.190834 1.3:+10540317 MS.gene86281:CDS
CTTGGTGTCTTATTTGAAAT+AGG 0.246453 1.3:-10539426 None:intergenic
CTTAATGGAGAAGGTTTAAC+TGG 0.266544 1.3:-10539228 None:intergenic
AAATAAGCTAGTTGGGAAAA+AGG 0.276009 1.3:+10539458 MS.gene86281:CDS
GTTGTGGGCTTTAGATAGTT+TGG 0.276367 1.3:-10539177 None:intergenic
ATCATTAGCTACCTTCTTAG+AGG 0.287986 1.3:-10539526 None:intergenic
ATCACAGCTTTGATCTTTGC+AGG 0.297205 1.3:-10539567 None:intergenic
ACTGCTAATTGTTGTTGCTT+TGG 0.334106 1.3:-10540311 None:intergenic
TTGTGGGCTTTAGATAGTTT+GGG 0.351132 1.3:-10539176 None:intergenic
GGAAACTGCAAGCAAGCTAT+TGG 0.368236 1.3:+10540379 MS.gene86281:CDS
GAAACTGCAAGCAAGCTATT+GGG 0.374785 1.3:+10540380 MS.gene86281:CDS
GAAAAGAAAGAAAATGAAAG+TGG 0.386555 1.3:+10540008 MS.gene86281:CDS
GAAAAGGAAGAAAATGAAAG+TGG 0.386555 1.3:+10540086 MS.gene86281:CDS
GAAAAGGAAGAAAATGAAAG+TGG 0.386555 1.3:+10540164 MS.gene86281:CDS
CAAAAGGAAGAAAATGAAAG+TGG 0.392066 1.3:+10539930 MS.gene86281:CDS
GATCTGCTTCACTAACTAAT+AGG 0.400951 1.3:+10539364 MS.gene86281:CDS
AGCTGTTTGAAACTTGTTGT+GGG 0.415610 1.3:-10539192 None:intergenic
AGAACAAGGAGGGGAAGAGA+AGG 0.423533 1.3:+10540229 MS.gene86281:CDS
AACAAGGTTCTTGCATTGGC+TGG 0.424301 1.3:+10540410 MS.gene86281:CDS
AAAGAAAGTAGTACACTTGT+TGG 0.425952 1.3:+10539030 MS.gene86281:CDS
GAGAAGGAAGCTGTTGAAGG+AGG 0.427486 1.3:+10540245 MS.gene86281:CDS
GATTTATCTCAAGTATCTAA+TGG 0.432672 1.3:+10540347 MS.gene86281:CDS
CGAAGTTTCTGTGATTGAAA+AGG 0.434643 1.3:+10540070 MS.gene86281:CDS
AGAAGGAAGCTGTTGAAGGA+GGG 0.434821 1.3:+10540246 MS.gene86281:CDS
CGAAGTTTCTGTGATTCAAA+AGG 0.437082 1.3:+10539914 MS.gene86281:CDS
CTCTAGGGCCAGGGGAAACA+AGG 0.437459 1.3:-10539338 None:intergenic
AGACCAAGAAGTAGAAGAAA+AGG 0.438530 1.3:+10539797 MS.gene86281:CDS
GACAAAAGAAGTTGATCGTT+CGG 0.453551 1.3:+10539881 MS.gene86281:CDS
GTTGAAATGACTTTCTCATA+AGG 0.454229 1.3:-10539768 None:intergenic
GAGGTAGGTGTTGTTGTGGA+TGG 0.457906 1.3:-10539108 None:intergenic
CTCTTGTTTGTTGTTGAGGT+AGG 0.465729 1.3:-10539123 None:intergenic
GCAGATCTTTCTCTAGGGCC+AGG 0.468580 1.3:-10539348 None:intergenic
GAAGAGAAGGAAGCTGTTGA+AGG 0.470608 1.3:+10540242 MS.gene86281:CDS
TGCAACTTCGCTTTCAACCT+CGG 0.472275 1.3:-10540135 None:intergenic
GAGCTGTTTGAAACTTGTTG+TGG 0.482060 1.3:-10539193 None:intergenic
GAAGGAAGCTGTTGAAGGAG+GGG 0.483098 1.3:+10540247 MS.gene86281:CDS
CACAAAGCCCTTGTTTCCCC+TGG 0.483515 1.3:+10539330 MS.gene86281:CDS
AACAACAATTAGCAGTAAAA+GGG 0.485734 1.3:+10540318 MS.gene86281:CDS
CTCTGAACTATTACATGAGC+AGG 0.488566 1.3:+10539713 MS.gene86281:CDS
GTTACTCTTGTTTGTTGTTG+AGG 0.494787 1.3:-10539127 None:intergenic
AAGAAAGTAGTACACTTGTT+GGG 0.496347 1.3:+10539031 MS.gene86281:CDS
AGTGAAGCAGATCTTTCTCT+AGG 0.499485 1.3:-10539354 None:intergenic
CGAAGTTGCAGTGAATGAAA+AGG 0.503697 1.3:+10540148 MS.gene86281:CDS
AGAAGTTGAAGTGATAAAAG+TGG 0.511607 1.3:+10539647 MS.gene86281:CDS
TGAAACTGAGGATGTTAAAG+AGG 0.512584 1.3:+10539614 MS.gene86281:CDS
GAAGAACAAGGTTCTTGCAT+TGG 0.514933 1.3:+10540406 MS.gene86281:CDS
AGTATCTAATGGTATGATTG+AGG 0.519661 1.3:+10540358 MS.gene86281:CDS
AGAAACTTCGCTTTCAACCT+CGG 0.521170 1.3:-10540057 None:intergenic
GTTAAGTTTGAAGATGAAAG+TGG 0.525002 1.3:-10539270 None:intergenic
TGTTGAGGTAGGTGTTGTTG+TGG 0.526424 1.3:-10539112 None:intergenic
GATCTTCTTCTTAATGGAGA+AGG 0.527948 1.3:-10539237 None:intergenic
AATAGTTCAGAGATGACATC+AGG 0.540223 1.3:-10539702 None:intergenic
CTTCTATCACAACAACCAAA+AGG 0.542669 1.3:+10539076 MS.gene86281:CDS
AGAAAAGGAGTCACAAGAAG+AGG 0.549465 1.3:+10539812 MS.gene86281:CDS
TACTTCTAAAACCTCTAAGA+AGG 0.551648 1.3:+10539515 MS.gene86281:CDS
CAGATCTTTCTCTAGGGCCA+GGG 0.557632 1.3:-10539347 None:intergenic
AAAAGAAAGAAAATGAAAGT+GGG 0.558618 1.3:+10540009 MS.gene86281:CDS
AAAAGGAAGAAAATGAAAGT+GGG 0.558618 1.3:+10540087 MS.gene86281:CDS
AAAAGGAAGAAAATGAAAGT+GGG 0.558618 1.3:+10540165 MS.gene86281:CDS
AAAAGGAAGAAAATGAAAGT+GGG 0.561058 1.3:+10539931 MS.gene86281:CDS
TTTGTGTAGTCTTGAAAGAG+AGG 0.569388 1.3:-10539313 None:intergenic
AGTTGAAGAAGCTAAGATTG+AGG 0.569432 1.3:+10540280 MS.gene86281:CDS
TCACAGCTTTGATCTTTGCA+GGG 0.589780 1.3:-10539566 None:intergenic
ACAATGGAGAAGAACAAGGA+GGG 0.590973 1.3:+10540219 MS.gene86281:CDS
CAGAAAAGTGAGAACAACAA+TGG 0.593589 1.3:+10540203 MS.gene86281:CDS
GTGAAGCAGATCTTTCTCTA+GGG 0.593867 1.3:-10539353 None:intergenic
AAACTGCAAGCAAGCTATTG+GGG 0.593923 1.3:+10540381 MS.gene86281:CDS
GTACACTTGTTGGGAATGAG+AGG 0.595873 1.3:+10539040 MS.gene86281:CDS
TCTAGGGCCAGGGGAAACAA+GGG 0.597582 1.3:-10539337 None:intergenic
CAATGGAGAAGAACAAGGAG+GGG 0.612731 1.3:+10540220 MS.gene86281:CDS
CTCATCAAGTGTTGAAACTG+AGG 0.626044 1.3:+10539602 MS.gene86281:CDS
AGATCTTTCTCTAGGGCCAG+GGG 0.629471 1.3:-10539346 None:intergenic
GCTATTGGGGAGGAAGAACA+AGG 0.635012 1.3:+10540394 MS.gene86281:CDS
AGAAGAAGTTGACCGTTCCG+AGG 0.641677 1.3:+10540118 MS.gene86281:CDS
CTTCGCTTTCAACCTCGGAA+CGG 0.645320 1.3:-10540130 None:intergenic
AGAAGAAGCTGATCATTCTG+AGG 0.655122 1.3:+10539962 MS.gene86281:CDS
AAAAGAAGTTGATCGTTCGG+AGG 0.657420 1.3:+10539884 MS.gene86281:CDS
AGAAGAAGTTGATCGTTCCG+AGG 0.659716 1.3:+10540040 MS.gene86281:CDS
AACAACAATGGAGAAGAACA+AGG 0.668634 1.3:+10540215 MS.gene86281:CDS
CTGCAAGCAAGCTATTGGGG+AGG 0.684251 1.3:+10540384 MS.gene86281:CDS
AACAATGGAGAAGAACAAGG+AGG 0.694534 1.3:+10540218 MS.gene86281:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAAGAAAATGAAAGT+GGG + chr1.3:10540009-10540028 MS.gene86281:CDS 20.0%
!!! TATTTGAAATAGGTTTTGAA+GGG - chr1.3:10539419-10539438 None:intergenic 20.0%
!!! TTATTTGAAATAGGTTTTGA+AGG - chr1.3:10539420-10539439 None:intergenic 20.0%
! AAAAGGAAGAAAATGAAAGT+GGG + chr1.3:10539931-10539950 MS.gene86281:CDS 25.0%
! AACAACAATTAGCAGTAAAA+GGG + chr1.3:10540318-10540337 MS.gene86281:CDS 25.0%
! ATGCAAAAAATAAGCTAGTT+GGG + chr1.3:10539451-10539470 MS.gene86281:CDS 25.0%
! GAAAAGAAAGAAAATGAAAG+TGG + chr1.3:10540008-10540027 MS.gene86281:CDS 25.0%
! GATTTATCTCAAGTATCTAA+TGG + chr1.3:10540347-10540366 MS.gene86281:CDS 25.0%
! TCTAATGATCTTCTTCTTAA+TGG - chr1.3:10539246-10539265 None:intergenic 25.0%
AAAGAAAGTAGTACACTTGT+TGG + chr1.3:10539030-10539049 MS.gene86281:CDS 30.0%
AAATAAGCTAGTTGGGAAAA+AGG + chr1.3:10539458-10539477 MS.gene86281:CDS 30.0%
AAGAAAGTAGTACACTTGTT+GGG + chr1.3:10539031-10539050 MS.gene86281:CDS 30.0%
AGAAGTTGAAGTGATAAAAG+TGG + chr1.3:10539647-10539666 MS.gene86281:CDS 30.0%
AGTATCTAATGGTATGATTG+AGG + chr1.3:10540358-10540377 MS.gene86281:CDS 30.0%
CAAAAGGAAGAAAATGAAAG+TGG + chr1.3:10539930-10539949 MS.gene86281:CDS 30.0%
CAACAACAATTAGCAGTAAA+AGG + chr1.3:10540317-10540336 MS.gene86281:CDS 30.0%
GAAAAGGAAGAAAATGAAAG+TGG + chr1.3:10540086-10540105 MS.gene86281:CDS 30.0%
GATGCAAAAAATAAGCTAGT+TGG + chr1.3:10539450-10539469 MS.gene86281:CDS 30.0%
GTTAAGTTTGAAGATGAAAG+TGG - chr1.3:10539273-10539292 None:intergenic 30.0%
GTTGAAATGACTTTCTCATA+AGG - chr1.3:10539771-10539790 None:intergenic 30.0%
! CTTTTATCACTTCAACTTCT+TGG - chr1.3:10539647-10539666 None:intergenic 30.0%
! GGAACATGATCAAATTTTTG+AGG + chr1.3:10539734-10539753 MS.gene86281:CDS 30.0%
!! AGAGAGGTTTCTTTTTGTTT+TGG - chr1.3:10539300-10539319 None:intergenic 30.0%
!! CTTGGTGTCTTATTTGAAAT+AGG - chr1.3:10539429-10539448 None:intergenic 30.0%
!! TACTTCTAAAACCTCTAAGA+AGG + chr1.3:10539515-10539534 MS.gene86281:CDS 30.0%
!!! AGCTTATTTTTTGCATCACT+TGG - chr1.3:10539447-10539466 None:intergenic 30.0%
AACAACAATGGAGAAGAACA+AGG + chr1.3:10540215-10540234 MS.gene86281:CDS 35.0%
AATAGTTCAGAGATGACATC+AGG - chr1.3:10539705-10539724 None:intergenic 35.0%
AGACCAAGAAGTAGAAGAAA+AGG + chr1.3:10539797-10539816 MS.gene86281:CDS 35.0%
AGTTGAAGAAGCTAAGATTG+AGG + chr1.3:10540280-10540299 MS.gene86281:CDS 35.0%
ATCATTAGCTACCTTCTTAG+AGG - chr1.3:10539529-10539548 None:intergenic 35.0%
CAGAAAAGTGAGAACAACAA+TGG + chr1.3:10540203-10540222 MS.gene86281:CDS 35.0%
CGAAGTTTCTGTGATTCAAA+AGG + chr1.3:10539914-10539933 MS.gene86281:CDS 35.0%
CGAAGTTTCTGTGATTGAAA+AGG + chr1.3:10540070-10540089 MS.gene86281:CDS 35.0%
CTTAATGGAGAAGGTTTAAC+TGG - chr1.3:10539231-10539250 None:intergenic 35.0%
CTTCTATCACAACAACCAAA+AGG + chr1.3:10539076-10539095 MS.gene86281:CDS 35.0%
GATCTGCTTCACTAACTAAT+AGG + chr1.3:10539364-10539383 MS.gene86281:CDS 35.0%
GATCTTCTTCTTAATGGAGA+AGG - chr1.3:10539240-10539259 None:intergenic 35.0%
TGAAACTGAGGATGTTAAAG+AGG + chr1.3:10539614-10539633 MS.gene86281:CDS 35.0%
! ACTCCTTTTCTTCTACTTCT+TGG - chr1.3:10539803-10539822 None:intergenic 35.0%
! ACTGCTAATTGTTGTTGCTT+TGG - chr1.3:10540314-10540333 None:intergenic 35.0%
! GACAAAAGAAGTTGATCGTT+CGG + chr1.3:10539881-10539900 MS.gene86281:CDS 35.0%
! TTGGGATGTTTTTCTCTGAT+GGG - chr1.3:10539161-10539180 None:intergenic 35.0%
! TTTGTGTAGTCTTGAAAGAG+AGG - chr1.3:10539316-10539335 None:intergenic 35.0%
!! AGCTGTTTGAAACTTGTTGT+GGG - chr1.3:10539195-10539214 None:intergenic 35.0%
!! CTTTTGGTTGTTGTGATAGA+AGG - chr1.3:10539078-10539097 None:intergenic 35.0%
!! GTTACTCTTGTTTGTTGTTG+AGG - chr1.3:10539130-10539149 None:intergenic 35.0%
!! TTGTGGGCTTTAGATAGTTT+GGG - chr1.3:10539179-10539198 None:intergenic 35.0%
!! TTTGGGATGTTTTTCTCTGA+TGG - chr1.3:10539162-10539181 None:intergenic 35.0%
!!! TGAAATAGGTTTTGAAGGGT+TGG - chr1.3:10539415-10539434 None:intergenic 35.0%
AAACTGCAAGCAAGCTATTG+GGG + chr1.3:10540381-10540400 MS.gene86281:CDS 40.0%
AACAATGGAGAAGAACAAGG+AGG + chr1.3:10540218-10540237 MS.gene86281:CDS 40.0%
ACAATGGAGAAGAACAAGGA+GGG + chr1.3:10540219-10540238 MS.gene86281:CDS 40.0%
AGAAAAGGAGTCACAAGAAG+AGG + chr1.3:10539812-10539831 MS.gene86281:CDS 40.0%
AGAAACTTCGCTTTCAACCT+CGG - chr1.3:10539904-10539923 None:intergenic 40.0%
AGAAGAAGCTGATCATTCTG+AGG + chr1.3:10539962-10539981 MS.gene86281:CDS 40.0%
AGTGAAGCAGATCTTTCTCT+AGG - chr1.3:10539357-10539376 None:intergenic 40.0%
CGAAGTTGCAGTGAATGAAA+AGG + chr1.3:10540148-10540167 MS.gene86281:CDS 40.0%
CTCTGAACTATTACATGAGC+AGG + chr1.3:10539713-10539732 MS.gene86281:CDS 40.0%
GAAACTGCAAGCAAGCTATT+GGG + chr1.3:10540380-10540399 MS.gene86281:CDS 40.0%
GAAGAACAAGGTTCTTGCAT+TGG + chr1.3:10540406-10540425 MS.gene86281:CDS 40.0%
GTGAAGCAGATCTTTCTCTA+GGG - chr1.3:10539356-10539375 None:intergenic 40.0%
! AAAAGAAGTTGATCGTTCGG+AGG + chr1.3:10539884-10539903 MS.gene86281:CDS 40.0%
! ATCACAGCTTTGATCTTTGC+AGG - chr1.3:10539570-10539589 None:intergenic 40.0%
! TCACAGCTTTGATCTTTGCA+GGG - chr1.3:10539569-10539588 None:intergenic 40.0%
! TGGGATGTTTTTCTCTGATG+GGG - chr1.3:10539160-10539179 None:intergenic 40.0%
!! CTCATCAAGTGTTGAAACTG+AGG + chr1.3:10539602-10539621 MS.gene86281:CDS 40.0%
!! CTCTTGTTTGTTGTTGAGGT+AGG - chr1.3:10539126-10539145 None:intergenic 40.0%
!! GAGCTGTTTGAAACTTGTTG+TGG - chr1.3:10539196-10539215 None:intergenic 40.0%
!! GTTGTGGGCTTTAGATAGTT+TGG - chr1.3:10539180-10539199 None:intergenic 40.0%
!! TGTTGTTGTGGATGGTTTTG+TGG - chr1.3:10539103-10539122 None:intergenic 40.0%
!!! GAAGGGTTGGTACTTTTAAC+TGG - chr1.3:10539402-10539421 None:intergenic 40.0%
AGAAGGAAGCTGTTGAAGGA+GGG + chr1.3:10540246-10540265 MS.gene86281:CDS 45.0%
CAATGGAGAAGAACAAGGAG+GGG + chr1.3:10540220-10540239 MS.gene86281:CDS 45.0%
GAAGAGAAGGAAGCTGTTGA+AGG + chr1.3:10540242-10540261 MS.gene86281:CDS 45.0%
GGAAACTGCAAGCAAGCTAT+TGG + chr1.3:10540379-10540398 MS.gene86281:CDS 45.0%
TGCAACTTCGCTTTCAACCT+CGG - chr1.3:10540138-10540157 None:intergenic 45.0%
! AACAAGGTTCTTGCATTGGC+TGG + chr1.3:10540410-10540429 MS.gene86281:CDS 45.0%
! AGAAGAAGTTGATCGTTCCG+AGG + chr1.3:10540040-10540059 MS.gene86281:CDS 45.0%
! GTACACTTGTTGGGAATGAG+AGG + chr1.3:10539040-10539059 MS.gene86281:CDS 45.0%
!! TGTTGAGGTAGGTGTTGTTG+TGG - chr1.3:10539115-10539134 None:intergenic 45.0%
!!! GGATGGTTTTGTGGTCCTTT+TGG - chr1.3:10539094-10539113 None:intergenic 45.0%
AGAACAAGGAGGGGAAGAGA+AGG + chr1.3:10540229-10540248 MS.gene86281:CDS 50.0%
AGAAGAAGTTGACCGTTCCG+AGG + chr1.3:10540118-10540137 MS.gene86281:CDS 50.0%
CTTCGCTTTCAACCTCGGAA+CGG - chr1.3:10540055-10540074 None:intergenic 50.0%
GAAGGAAGCTGTTGAAGGAG+GGG + chr1.3:10540247-10540266 MS.gene86281:CDS 50.0%
GAGAAGGAAGCTGTTGAAGG+AGG + chr1.3:10540245-10540264 MS.gene86281:CDS 50.0%
GCTATTGGGGAGGAAGAACA+AGG + chr1.3:10540394-10540413 MS.gene86281:CDS 50.0%
!! AGATCTTTCTCTAGGGCCAG+GGG - chr1.3:10539349-10539368 None:intergenic 50.0%
!! CAGATCTTTCTCTAGGGCCA+GGG - chr1.3:10539350-10539369 None:intergenic 50.0%
!! GAGGTAGGTGTTGTTGTGGA+TGG - chr1.3:10539111-10539130 None:intergenic 50.0%
CTGCAAGCAAGCTATTGGGG+AGG + chr1.3:10540384-10540403 MS.gene86281:CDS 55.0%
! CACAAAGCCCTTGTTTCCCC+TGG + chr1.3:10539330-10539349 MS.gene86281:CDS 55.0%
! TCTAGGGCCAGGGGAAACAA+GGG - chr1.3:10539340-10539359 None:intergenic 55.0%
!! GCAGATCTTTCTCTAGGGCC+AGG - chr1.3:10539351-10539370 None:intergenic 55.0%
!! CTCTAGGGCCAGGGGAAACA+AGG - chr1.3:10539341-10539360 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 10539015 10540469 10539015 ID=MS.gene86281
chr1.3 mRNA 10539015 10540469 10539015 ID=MS.gene86281.t1;Parent=MS.gene86281
chr1.3 exon 10539015 10540469 10539015 ID=MS.gene86281.t1.exon1;Parent=MS.gene86281.t1
chr1.3 CDS 10539015 10540469 10539015 ID=cds.MS.gene86281.t1;Parent=MS.gene86281.t1
Gene Sequence

>MS.gene86281

ATGGCAACAAAAGCTAAAGAAAGTAGTACACTTGTTGGGAATGAGAGGAAGAAGACAGCACCTTCTATCACAACAACCAAAAGGACCACAAAACCATCCACAACAACACCTACCTCAACAACAAACAAGAGTAACTCAAGCACCCCATCAGAGAAAAACATCCCAAACTATCTAAAGCCCACAACAAGTTTCAAACAGCTCAAAACTGAGATTCCAGTTAAACCTTCTCCATTAAGAAGAAGATCATTAGACAAACCACTTTCATCTTCAAACTTAACAGCACCAAAACAAAAAGAAACCTCTCTTTCAAGACTACACAAAGCCCTTGTTTCCCCTGGCCCTAGAGAAAGATCTGCTTCACTAACTAATAGGTCTTCTAATGTTCCAGTTAAAAGTACCAACCCTTCAAAACCTATTTCAAATAAGACACCAAGTGATGCAAAAAATAAGCTAGTTGGGAAAAAGGTTATGACTAAGAGTACTCTTAGTAGAAATAATTCTACTTCTAAAACCTCTAAGAAGGTAGCTAATGATCATGCTTCTTCTGATATCCCTGCAAAGATCAAAGCTGTGATTACAGATTCTGACTCATCAAGTGTTGAAACTGAGGATGTTAAAGAGGTTACAAACCAAGAAGTTGAAGTGATAAAAGTGGAGAATGAAGAATATGTATCTCATGAGATTTCACCTGATGTCATCTCTGAACTATTACATGAGCAGGAACATGATCAAATTTTTGAGGATTCTGACATACCTTATGAGAAAGTCATTTCAACAGTTTCAGACCAAGAAGTAGAAGAAAAGGAGTCACAAGAAGAGGAACATGAAGTTGAGCATGAAGAGAAAAAGAACAATAATGAGAATCAGACAAAAGAAGTTGATCGTTCGGAGGTTGAAAGCGAAGTTTCTGTGATTCAAAAGGAAGAAAATGAAAGTGGGAATCAGATAGAAGAAGCTGATCATTCTGAGGTTGAAAGTGAAGTTGCTATGAATGAAAAGAAAGAAAATGAAAGTGGGAATCAGACAGAAGAAGTTGATCGTTCCGAGGTTGAAAGCGAAGTTTCTGTGATTGAAAAGGAAGAAAATGAAAGTGGGAATCAGACAGAAGAAGTTGACCGTTCCGAGGTTGAAAGCGAAGTTGCAGTGAATGAAAAGGAAGAAAATGAAAGTGGGAATGTGATAGAAGATCAGAAAAGTGAGAACAACAATGGAGAAGAACAAGGAGGGGAAGAGAAGGAAGCTGTTGAAGGAGGGGTTAGTGAAGAAGTTGAAGAAGCTAAGATTGAGGTAGCACAACCAAAGCAACAACAATTAGCAGTAAAAGGGAAGAATGATTTATCTCAAGTATCTAATGGTATGATTGAGGAAACTGCAAGCAAGCTATTGGGGAGGAAGAACAAGGTTCTTGCATTGGCTGGAGCATTTCAAACTGTCATTGATCATCAAATCAAATGA
Protein sequence

>MS.gene86281.t1

MATKAKESSTLVGNERKKTAPSITTTKRTTKPSTTTPTSTTNKSNSSTPSEKNIPNYLKPTTSFKQLKTEIPVKPSPLRRRSLDKPLSSSNLTAPKQKETSLSRLHKALVSPGPRERSASLTNRSSNVPVKSTNPSKPISNKTPSDAKNKLVGKKVMTKSTLSRNNSTSKTSKKVANDHASSDIPAKIKAVITDSDSSSVETEDVKEVTNQEVEVIKVENEEYVSHEISPDVISELLHEQEHDQIFEDSDIPYEKVISTVSDQEVEEKESQEEEHEVEHEEKKNNNENQTKEVDRSEVESEVSVIQKEENESGNQIEEADHSEVESEVAMNEKKENESGNQTEEVDRSEVESEVSVIEKEENESGNQTEEVDRSEVESEVAVNEKEENESGNVIEDQKSENNNGEEQGGEEKEAVEGGVSEEVEEAKIEVAQPKQQQLAVKGKNDLSQVSNGMIEETASKLLGRKNKVLALAGAFQTVIDHQIK