Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05952.t1 | XP_013447314.1 | 89.9 | 207 | 21 | 0 | 1 | 207 | 1 | 207 | 3.40E-100 | 374.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| No data available in table | |||||||||||
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05952.t1 | I3S9W1 | 89.9 | 207 | 21 | 0 | 1 | 207 | 1 | 207 | 2.5e-100 | 374.4 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene05952 | 0.809197 | 2.13E-50 | -1.69E-46 |
| MS.gene04919 | MS.gene05952 | 0.827277 | 1.71E-54 | -1.69E-46 |
| MS.gene04920 | MS.gene05952 | 0.846302 | 2.36E-59 | -1.69E-46 |
| MS.gene049254 | MS.gene05952 | 0.808299 | 3.32E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene05952 | 0.879034 | 1.73E-69 | -1.69E-46 |
| MS.gene049419 | MS.gene05952 | -0.802584 | 5.23E-49 | -1.69E-46 |
| MS.gene049499 | MS.gene05952 | 0.86374 | 2.01E-64 | -1.69E-46 |
| MS.gene049680 | MS.gene05952 | 0.814483 | 1.51E-51 | -1.69E-46 |
| MS.gene04998 | MS.gene05952 | 0.816986 | 4.17E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene05952 | 0.833055 | 6.64E-56 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05952.t1 | MTR_8g102755 | 89.855 | 207 | 21 | 0 | 1 | 207 | 1 | 207 | 2.38e-140 | 390 |
Find 31 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGGTCTAACGTAAAAC+AGG | 0.453639 | 4.3:-2601680 | MS.gene05952:CDS |
| AAATAATGGTGTAATAGATG+AGG | 0.639630 | 4.3:-2601359 | MS.gene05952:CDS |
| AAGACAGAAACAAAGAAATT+TGG | 0.303541 | 4.3:-2601601 | MS.gene05952:CDS |
| AATGGTGTTCCGCCTGACGG+TGG | 0.691121 | 4.3:-2601316 | MS.gene05952:CDS |
| ACAACTTGGAGTTGAAGAGA+AGG | 0.600247 | 4.3:-2601419 | MS.gene05952:CDS |
| ACTGTTCATGTTGATACTTC+AGG | 0.350166 | 4.3:-2601142 | MS.gene05952:CDS |
| AGAATGGAGTATCAATCTCT+TGG | 0.471194 | 4.3:-2601718 | MS.gene05952:CDS |
| AGTATTTATGAAGTCACGTA+TGG | 0.536293 | 4.3:-2601244 | MS.gene05952:CDS |
| ATAAATTGTTTGGACGGAAA+GGG | 0.528425 | 4.3:-2601546 | MS.gene05952:CDS |
| ATCAAGCTTGTTGCTGGATG+TGG | 0.461106 | 4.3:-2601571 | MS.gene05952:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACAAGATGCAGAAAAATG+AGG | - | chr4.3:2601434-2601453 | MS.gene05952:CDS | 30.0% | |
| AGTATTTATGAAGTCACGTA+TGG | - | chr4.3:2601585-2601604 | MS.gene05952:CDS | 30.0% | |
| ATAAATTGTTTGGACGGAAA+GGG | - | chr4.3:2601283-2601302 | MS.gene05952:CDS | 30.0% | |
| CAAAGCTTTCATGATGAAAA+TGG | - | chr4.3:2601495-2601514 | MS.gene05952:CDS | 30.0% | |
| GTAGACGAAGAAATTAATAC+AGG | - | chr4.3:2601660-2601679 | MS.gene05952:CDS | 30.0% | |
| AAAAGGGTCTAACGTAAAAC+AGG | - | chr4.3:2601149-2601168 | MS.gene05952:CDS | 35.0% | |
| ACTGTTCATGTTGATACTTC+AGG | - | chr4.3:2601687-2601706 | MS.gene05952:CDS | 35.0% | |
| CAGGTTTGCAATCAAGAAAT+AGG | - | chr4.3:2601706-2601725 | MS.gene05952:CDS | 35.0% | |
| CATAAATTGTTTGGACGGAA+AGG | - | chr4.3:2601282-2601301 | MS.gene05952:CDS | 35.0% | |
| GGATGTGGACATAAATTGTT+TGG | - | chr4.3:2601273-2601292 | MS.gene05952:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 2601114 | 2601737 | 2601114 | ID=MS.gene05952 |
| chr4.3 | mRNA | 2601114 | 2601737 | 2601114 | ID=MS.gene05952.t1;Parent=MS.gene05952 |
| chr4.3 | exon | 2601114 | 2601737 | 2601114 | ID=MS.gene05952.t1.exon1;Parent=MS.gene05952.t1 |
| chr4.3 | CDS | 2601114 | 2601737 | 2601114 | ID=cds.MS.gene05952.t1;Parent=MS.gene05952.t1 |
| Gene Sequence |
>MS.gene05952 ATGGAGTATCAATCTCTTGGAAGGTACCAAAAACCAAAAGGGTCTAACGTAAAACAGGTATTGAAGATGATGCTACTTTTGGCTGTTTGTGCCTGGTTGTTATATCAAATCAAGAAGACAGAAACAAAGAAATTTGGTATTCAAATCAAGCTTGTTGCTGGATGTGGACATAAATTGTTTGGACGGAAAGGGATGCTATCACGTCCCGATGAAAGAGCTTTTCCTGACTCAGGAAAGGTAGATTCTGTTGGAGAAGTAACAAATGAGTTTAAAAGTAATGACAAAGAAAAAGAAGTACAACTTGGAGTTGAAGAGAAGGGAAACAAGATGCAGAAAAATGAGGTGGAGAGTGCCGCAAATAATGGTGTAATAGATGAGGTCCAAAGCTTTCATGATGAAAATGGTGTTCCGCCTGACGGTGGTGAAGAAAACATGTCAACTTTTAGAATAGTAAATTGGCTTAAAAAGATCAGTATTTATGAAGTCACGTATGGAGAAGATAATGATATTGAAATGAATTTGGAAGGAACGACGAATGCTACAACAGTAGACGAAGAAATTAATACAGGAACTACTGTTCATGTTGATACTTCAGGTTTGCAATCAAGAAATAGGTGGAGATAA |
| Protein sequence |
>MS.gene05952.t1 MEYQSLGRYQKPKGSNVKQVLKMMLLLAVCAWLLYQIKKTETKKFGIQIKLVAGCGHKLFGRKGMLSRPDERAFPDSGKVDSVGEVTNEFKSNDKEKEVQLGVEEKGNKMQKNEVESAANNGVIDEVQSFHDENGVPPDGGEENMSTFRIVNWLKKISIYEVTYGEDNDIEMNLEGTTNATTVDEEINTGTTVHVDTSGLQSRNRWR |
