AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86318

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86318.t1 MTR_1g010120 99.140 465 3 1 1 465 13 476 0.0 942
MS.gene86318.t1 MTR_3g108600 73.548 465 103 2 1 464 17 462 0.0 709
MS.gene86318.t1 MTR_1g053320 52.754 472 212 5 1 462 16 486 0.0 518
MS.gene86318.t1 MTR_4g124440 59.302 430 172 3 14 441 27 455 0.0 517
MS.gene86318.t1 MTR_6g032820 59.346 428 165 5 1 421 12 437 4.75e-178 506
MS.gene86318.t1 MTR_4g112025 52.838 458 208 4 14 464 30 486 1.76e-175 502
MS.gene86318.t1 MTR_7g085390 55.580 457 194 5 12 464 23 474 2.88e-172 493
MS.gene86318.t1 MTR_8g092070 52.863 454 206 4 12 459 31 482 2.56e-169 489
MS.gene86318.t1 MTR_2g027560 53.630 427 194 2 15 439 28 452 7.94e-164 471
MS.gene86318.t1 MTR_3g095050 50.448 446 216 4 12 455 31 473 1.19e-159 461
MS.gene86318.t1 MTR_3g031770 45.909 440 232 5 14 451 26 461 5.08e-135 398
MS.gene86318.t1 MTR_4g134280 52.473 364 168 3 104 464 2 363 3.45e-131 384
MS.gene86318.t1 MTR_4g069940 35.147 441 258 15 15 437 29 459 1.31e-68 226
MS.gene86318.t1 MTR_4g088155 33.987 459 275 17 3 441 11 461 1.73e-68 226
MS.gene86318.t1 MTR_8g012400 33.769 459 271 16 3 441 17 462 3.16e-67 223
MS.gene86318.t1 MTR_1g031900 35.172 435 257 15 15 437 24 445 7.70e-67 223
MS.gene86318.t1 MTR_1g031900 35.172 435 257 15 15 437 24 445 1.42e-66 222
MS.gene86318.t1 MTR_6g088885 31.659 458 281 14 2 437 7 454 7.49e-63 212
MS.gene86318.t1 MTR_8g085720 31.448 442 265 14 15 437 26 448 9.16e-62 209
MS.gene86318.t1 MTR_3g116510 32.569 436 272 15 15 437 28 454 6.64e-60 203
MS.gene86318.t1 MTR_1g056640 30.687 466 275 16 11 437 34 490 4.19e-59 202
MS.gene86318.t1 MTR_5g085580 32.613 463 265 18 8 437 18 466 1.98e-58 199
MS.gene86318.t1 MTR_5g078200 32.972 461 262 19 1 437 7 444 1.05e-57 201
MS.gene86318.t1 MTR_3g065460 31.416 452 281 15 1 437 8 445 1.20e-57 198
MS.gene86318.t1 MTR_3g083580 29.817 436 278 15 15 437 26 446 1.65e-57 197
MS.gene86318.t1 MTR_7g053500 30.942 446 270 17 16 437 27 458 8.17e-57 195
MS.gene86318.t1 MTR_4g010200 37.500 328 189 10 13 332 31 350 3.58e-55 189
MS.gene86318.t1 MTR_7g081350 36.308 325 186 10 14 332 26 335 8.96e-55 186
MS.gene86318.t1 MTR_1g007810 38.416 341 181 15 3 332 4 326 2.38e-54 186
MS.gene86318.t1 MTR_5g078200 32.483 431 246 18 29 437 4 411 4.92e-54 191
MS.gene86318.t1 MTR_7g026340 33.258 445 266 19 8 435 16 446 1.92e-53 186
MS.gene86318.t1 MTR_1g052430 35.093 322 198 7 14 333 31 343 3.74e-53 184
MS.gene86318.t1 MTR_1g077990 35.780 327 190 12 15 332 31 346 5.01e-53 183
MS.gene86318.t1 MTR_4g084480 34.731 334 201 12 11 336 28 352 8.71e-53 182
MS.gene86318.t1 MTR_4g084480 34.731 334 201 12 11 336 21 345 1.13e-52 182
MS.gene86318.t1 MTR_4g084480 34.731 334 201 12 11 336 28 352 1.35e-52 182
MS.gene86318.t1 MTR_2g034440 34.395 314 197 6 10 322 17 322 1.14e-51 178
MS.gene86318.t1 MTR_8g012400 32.741 394 232 16 68 441 2 382 5.15e-51 177
MS.gene86318.t1 MTR_7g081370 34.226 336 189 10 2 332 15 323 1.01e-50 175
MS.gene86318.t1 MTR_4g076255 36.842 323 182 13 15 333 25 329 1.16e-50 175
MS.gene86318.t1 MTR_1g022160 29.130 460 280 14 15 437 24 474 3.63e-50 178
MS.gene86318.t1 MTR_1g022160 28.913 460 281 14 15 437 24 474 4.45e-50 177
MS.gene86318.t1 MTR_4g052970 33.516 364 212 15 6 357 18 363 4.72e-50 175
MS.gene86318.t1 MTR_4g132280 29.655 435 281 14 15 437 45 466 4.97e-50 178
MS.gene86318.t1 MTR_2g034480 33.535 331 207 7 15 342 27 347 8.87e-50 174
MS.gene86318.t1 MTR_4g083500 30.921 456 268 15 15 437 44 485 1.85e-49 177
MS.gene86318.t1 MTR_2g034470 33.133 332 213 6 10 340 25 348 2.34e-49 172
MS.gene86318.t1 MTR_3g080410 35.780 327 192 10 15 332 26 343 4.16e-49 172
MS.gene86318.t1 MTR_8g102340 33.245 379 227 14 73 437 52 418 5.65e-49 174
MS.gene86318.t1 MTR_4g114850 35.258 329 195 10 13 332 24 343 9.55e-49 171
MS.gene86318.t1 MTR_4g114850 35.258 329 195 10 13 332 24 343 1.07e-48 171
MS.gene86318.t1 MTR_4g076570 33.750 320 201 7 15 332 22 332 1.15e-48 171
MS.gene86318.t1 MTR_4g114850 35.258 329 195 10 13 332 24 343 1.18e-48 171
MS.gene86318.t1 MTR_1g022160 28.728 456 279 14 15 433 24 470 1.75e-48 173
MS.gene86318.t1 MTR_2g034470 33.981 309 195 6 33 340 13 313 4.68e-48 168
MS.gene86318.t1 MTR_7g053500 31.026 390 239 16 68 437 2 381 1.57e-46 166
MS.gene86318.t1 MTR_4g076500 33.134 335 208 9 3 333 66 388 2.30e-46 165
MS.gene86318.t1 MTR_2g019345 34.146 328 196 12 16 334 25 341 3.50e-46 165
MS.gene86318.t1 MTR_2g086530 32.768 354 207 16 1 338 11 349 2.09e-44 160
MS.gene86318.t1 MTR_2g086530 32.239 335 200 14 16 338 9 328 1.63e-43 157
MS.gene86318.t1 MTR_4g076470 33.127 323 200 9 12 333 20 327 4.09e-43 155
MS.gene86318.t1 MTR_4g076430 33.127 323 200 8 12 333 20 327 7.38e-43 154
MS.gene86318.t1 MTR_1g022160 31.661 319 203 9 15 325 24 335 3.74e-41 152
MS.gene86318.t1 MTR_3g116510 32.722 327 199 13 125 437 1 320 1.46e-40 148
MS.gene86318.t1 MTR_4g076440 31.269 323 206 9 12 333 20 327 3.29e-40 147
MS.gene86318.t1 MTR_4g076490 31.875 320 201 10 15 332 97 401 2.99e-39 147
MS.gene86318.t1 MTR_5g081720 28.758 306 200 11 32 330 54 348 7.02e-28 114
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86318.t1 AT4G31140 67.312 465 151 1 1 465 13 476 0.0 669
MS.gene86318.t1 AT5G58090 67.366 429 139 1 15 442 22 450 0.0 611
MS.gene86318.t1 AT5G20870 57.080 452 181 5 2 443 17 465 1.24e-175 502
MS.gene86318.t1 AT3G24330 54.875 441 192 5 15 450 39 477 6.04e-172 493
MS.gene86318.t1 AT1G64760 55.530 434 186 5 12 441 22 452 1.64e-166 478
MS.gene86318.t1 AT1G64760 55.530 434 186 5 12 441 22 452 1.64e-166 478
MS.gene86318.t1 AT3G04010 54.214 439 195 4 12 446 30 466 3.22e-166 478
MS.gene86318.t1 AT5G18220 52.677 467 204 7 11 464 26 488 9.54e-166 476
MS.gene86318.t1 AT2G19440 54.525 442 194 5 4 441 10 448 1.57e-165 475
MS.gene86318.t1 AT2G19440 54.525 442 194 5 4 441 10 448 1.57e-165 475
MS.gene86318.t1 AT5G64790 51.376 436 209 2 12 445 25 459 1.01e-162 469
MS.gene86318.t1 AT4G17180 51.693 443 199 6 3 439 9 442 1.90e-154 447
MS.gene86318.t1 AT5G58480 43.807 436 239 5 12 446 23 453 1.37e-128 381
MS.gene86318.t1 AT2G05790 34.423 459 258 16 11 437 21 468 4.02e-69 228
MS.gene86318.t1 AT2G01630 34.318 440 254 15 15 437 23 444 5.39e-69 228
MS.gene86318.t1 AT2G01630 34.713 435 247 16 15 431 23 438 2.44e-68 225
MS.gene86318.t1 AT4G26830 32.955 440 269 14 13 437 23 451 6.99e-68 224
MS.gene86318.t1 AT4G26830 32.955 440 269 14 13 437 27 455 7.33e-68 224
MS.gene86318.t1 AT5G55180 31.519 441 277 13 13 437 25 456 7.29e-65 216
MS.gene86318.t1 AT5G55180 31.724 435 272 13 13 431 25 450 6.16e-64 214
MS.gene86318.t1 AT5G56590 31.746 441 274 15 13 437 24 453 5.01e-62 210
MS.gene86318.t1 AT3G07320 32.658 444 269 17 13 437 23 455 4.90e-61 206
MS.gene86318.t1 AT3G55430 34.086 443 256 22 12 437 26 449 2.53e-60 204
MS.gene86318.t1 AT1G66250 32.110 436 268 14 15 437 32 452 1.66e-59 203
MS.gene86318.t1 AT3G57270 36.025 322 195 7 13 332 25 337 6.32e-58 194
MS.gene86318.t1 AT1G11820 29.841 439 275 14 15 437 44 465 1.05e-57 199
MS.gene86318.t1 AT5G24318 31.377 443 273 18 13 438 28 456 1.56e-57 197
MS.gene86318.t1 AT4G14080 30.872 447 270 17 15 437 42 473 1.82e-56 194
MS.gene86318.t1 AT1G11820 29.792 433 271 14 15 431 44 459 2.15e-56 194
MS.gene86318.t1 AT3G23770 31.333 450 267 18 15 437 37 471 4.55e-56 193
MS.gene86318.t1 AT5G24318 31.422 436 268 18 13 431 28 449 7.40e-56 192
MS.gene86318.t1 AT3G13560 30.717 446 261 15 15 437 26 446 1.96e-55 192
MS.gene86318.t1 AT3G13560 30.717 446 261 15 15 437 26 446 1.96e-55 192
MS.gene86318.t1 AT3G13560 30.717 446 261 15 15 437 26 446 1.96e-55 192
MS.gene86318.t1 AT3G13560 30.717 446 261 15 15 437 26 446 1.96e-55 192
MS.gene86318.t1 AT3G13560 30.717 446 261 15 15 437 26 446 1.96e-55 192
MS.gene86318.t1 AT2G16230 32.037 437 271 14 15 437 25 449 1.27e-54 190
MS.gene86318.t1 AT4G29360 29.449 472 283 15 15 448 27 486 6.61e-54 189
MS.gene86318.t1 AT4G29360 29.449 472 283 15 15 448 37 496 7.46e-54 189
MS.gene86318.t1 AT4G34480 32.200 441 262 15 15 437 26 447 9.53e-54 188
MS.gene86318.t1 AT3G57260 36.646 322 187 10 13 332 30 336 1.73e-53 183
MS.gene86318.t1 AT3G57260 36.646 322 187 10 13 332 46 352 2.72e-53 183
MS.gene86318.t1 AT2G01630 33.687 377 216 14 15 376 23 380 3.62e-53 183
MS.gene86318.t1 AT3G15800 34.615 338 208 9 2 332 30 361 1.55e-52 182
MS.gene86318.t1 AT2G39640 33.259 451 263 22 2 439 19 444 4.44e-52 184
MS.gene86318.t1 AT4G16260 32.267 344 218 9 1 340 8 340 3.75e-51 177
MS.gene86318.t1 AT4G18340 32.033 359 221 13 2 349 21 367 3.64e-50 175
MS.gene86318.t1 AT4G16260 33.227 313 196 8 30 340 10 311 5.01e-50 173
MS.gene86318.t1 AT4G18340 32.033 359 221 13 2 349 21 367 7.82e-50 175
MS.gene86318.t1 AT1G30080 31.722 331 207 11 11 332 32 352 2.02e-48 171
MS.gene86318.t1 AT1G30080 31.722 331 207 11 11 332 32 352 2.40e-48 171
MS.gene86318.t1 AT5G20330 34.743 331 195 11 9 332 28 344 8.18e-48 168
MS.gene86318.t1 AT5G24318 33.435 329 202 13 13 334 28 346 1.06e-47 168
MS.gene86318.t1 AT1G33220 34.641 306 183 9 9 308 28 322 1.18e-47 167
MS.gene86318.t1 AT2G27500 32.927 328 202 11 14 332 28 346 1.68e-47 168
MS.gene86318.t1 AT5G42720 35.196 358 209 14 15 361 26 371 1.93e-47 169
MS.gene86318.t1 AT2G27500 33.129 326 200 11 14 330 28 344 3.23e-47 167
MS.gene86318.t1 AT5G20390 33.538 325 195 10 15 332 33 343 3.40e-47 166
MS.gene86318.t1 AT1G32860 33.435 329 198 12 15 332 29 347 3.66e-47 168
MS.gene86318.t1 AT2G26600 33.030 330 203 10 12 332 31 351 6.50e-47 167
MS.gene86318.t1 AT5G20340 34.154 325 193 11 15 332 43 353 1.71e-46 165
MS.gene86318.t1 AT3G57240 33.022 321 197 7 12 330 32 336 2.03e-45 161
MS.gene86318.t1 AT5G42100 33.828 337 201 13 10 334 22 348 4.13e-44 160
MS.gene86318.t1 AT5G42100 33.828 337 201 13 10 334 22 348 4.25e-44 160
MS.gene86318.t1 AT4G34480 35.826 321 186 10 15 325 26 336 1.84e-43 157
MS.gene86318.t1 AT1G11820 29.545 352 219 11 15 353 44 379 1.50e-42 155
MS.gene86318.t1 AT3G61810 31.212 330 200 10 15 332 55 369 1.31e-41 152
MS.gene86318.t1 AT1G77780 29.565 345 224 8 2 339 7 339 1.70e-38 144
MS.gene86318.t1 AT5G20560 31.210 314 197 9 15 322 34 334 2.44e-38 142
MS.gene86318.t1 AT4G34480 31.339 351 206 14 105 437 3 336 8.00e-35 134
MS.gene86318.t1 AT2G27500 32.727 275 167 11 65 330 1 266 8.22e-35 132
MS.gene86318.t1 AT1G77790 30.000 310 199 9 29 332 44 341 3.36e-34 131
MS.gene86318.t1 AT3G46570 33.233 331 197 13 15 334 27 344 6.04e-34 131
MS.gene86318.t1 AT5G24318 29.358 327 205 13 128 438 1 317 1.33e-33 129
MS.gene86318.t1 AT3G55780 26.426 333 218 10 15 332 31 351 2.89e-33 129
MS.gene86318.t1 AT2G26600 32.707 266 161 10 76 332 1 257 9.06e-32 123

Find 111 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAGCATCAAGAATATT+TGG 0.148229 1.3:+10089553 None:intergenic
TGCATAGTCGACTGGAAAAT+TGG 0.169551 1.3:+10087551 None:intergenic
ACCGATCTCTCCAACAATTA+TGG 0.179022 1.3:+10087410 None:intergenic
ATCTTTCCGTTGTCGTTTAA+AGG 0.202599 1.3:+10087507 None:intergenic
CGCTCTTATAAAAGCTGGTT+TGG 0.202719 1.3:-10087760 MS.gene86318:CDS
GACTAGTGCAGTCTGCATTT+TGG 0.234926 1.3:+10087031 None:intergenic
GGATAAATGTTCACGGTAAA+TGG 0.262811 1.3:+10087597 None:intergenic
AAATGATTTGCTTTATACTA+TGG 0.263703 1.3:-10089497 MS.gene86318:CDS
TTTGAAGAAATCTGGTATTC+AGG 0.267033 1.3:-10089536 MS.gene86318:CDS
TCTCAATGGAGTCCCCTTTC+CGG 0.272611 1.3:+10087308 None:intergenic
CTTGATGCTTTGAAGAAATC+TGG 0.297702 1.3:-10089544 MS.gene86318:CDS
TTTCAAAATACACCACTTCT+TGG 0.304045 1.3:+10087107 None:intergenic
CTAACTATGATACTCTAGTA+TGG 0.317827 1.3:-10087464 MS.gene86318:CDS
AATCAGTTTGGATCATGATT+TGG 0.323082 1.3:+10086848 None:intergenic
TTCTCCTCGCGGAATCAGTT+TGG 0.344088 1.3:+10086836 None:intergenic
GAAATCTGGTATTCAGGTTA+TGG 0.346498 1.3:-10089530 MS.gene86318:CDS
TCTTCATAAAGACTAATAAA+AGG 0.350618 1.3:+10087576 None:intergenic
GTGCAACTTGATCACTGTTC+AGG 0.355760 1.3:+10087073 None:intergenic
CAGGTAACTTTGAACGTCAT+TGG 0.361509 1.3:-10087224 MS.gene86318:CDS
AGGTAACTTTGAACGTCATT+GGG 0.365853 1.3:-10087223 MS.gene86318:CDS
CATCTGGTGGAGTAGATATC+AGG 0.372328 1.3:-10089417 MS.gene86318:intron
CCGATCTCTCCAACAATTAT+GGG 0.378662 1.3:+10087411 None:intergenic
CCAATGGTGAGTTGGTATTT+CGG 0.383764 1.3:+10087177 None:intergenic
GTTTCAGCTCATGTTTCATC+TGG 0.386117 1.3:-10089433 MS.gene86318:CDS
ACCCTTCCGATGGCGACTTT+AGG 0.386656 1.3:-10087677 MS.gene86318:CDS
TTCATGACTCGATTTATTGC+CGG 0.399249 1.3:-10087327 MS.gene86318:CDS
TTCAAAATACACCACTTCTT+GGG 0.402891 1.3:+10087108 None:intergenic
GTGAAAATGTTAAAAGATAA+TGG 0.403732 1.3:-10089607 MS.gene86318:CDS
GTGTTGGAAGTGGGTGGAAT+TGG 0.404650 1.3:-10089679 MS.gene86318:CDS
GGGTGGAATTGGAGTGAACT+GGG 0.423782 1.3:-10089668 MS.gene86318:CDS
TGGGTGGAATTGGAGTGAAC+TGG 0.426991 1.3:-10089669 MS.gene86318:CDS
ACTCGATTTATTGCCGGAAA+GGG 0.427868 1.3:-10087321 MS.gene86318:CDS
TCCATTGAGACCTGGTCCTA+TGG 0.429507 1.3:-10087295 MS.gene86318:CDS
CCCATAATTGTTGGAGAGAT+CGG 0.432044 1.3:-10087411 MS.gene86318:CDS
GATGGCGCTTGCAAATTTCC+CGG 0.432957 1.3:-10086919 MS.gene86318:CDS
GCATAGTCGACTGGAAAATT+GGG 0.440341 1.3:+10087552 None:intergenic
GCTCTTATAAAAGCTGGTTT+GGG 0.442512 1.3:-10087759 MS.gene86318:CDS
ATAGTATAAAGCAAATCATT+TGG 0.452724 1.3:+10089499 None:intergenic
GGTAACATGCCCATAATTGT+TGG 0.454864 1.3:-10087420 MS.gene86318:CDS
CAAAACGCTCTTATAAAAGC+TGG 0.455309 1.3:-10087765 MS.gene86318:CDS
TGCGTTTAATAGCTACTATC+AGG 0.464738 1.3:-10086956 MS.gene86318:CDS
CAGGTGAATGATCAGGTTGA+TGG 0.470214 1.3:-10086937 MS.gene86318:CDS
TCAGCTCATGTTTCATCTGG+TGG 0.473275 1.3:-10089430 MS.gene86318:CDS
CCGTTTCTGTCTCCGTCTGT+AGG 0.478583 1.3:+10087384 None:intergenic
GATGTGTATCAGAGCTCATC+AGG 0.478752 1.3:-10087702 MS.gene86318:CDS
CTACTATCAGGTGAATGATC+AGG 0.483380 1.3:-10086944 MS.gene86318:CDS
TGATACACATCAGCATTTGA+AGG 0.486649 1.3:+10087714 None:intergenic
GCGTTGAATAAGAATGGCTT+TGG 0.487733 1.3:-10087441 MS.gene86318:CDS
GTCAGTGACAGTAGAAAGAC+CGG 0.491584 1.3:+10086900 None:intergenic
TGACAGAGATCCTTCAACTC+AGG 0.492465 1.3:-10086881 MS.gene86318:CDS
CCGAAATACCAACTCACCAT+TGG 0.493738 1.3:-10087177 MS.gene86318:CDS
CCCGTTGGTTCTTGATCCAA+TGG 0.499872 1.3:+10087161 None:intergenic
AATGCAGACTGCACTAGTCT+TGG 0.500089 1.3:-10087027 MS.gene86318:CDS
ACCACAGTAGATGGTGGTAA+TGG 0.502268 1.3:+10089628 None:intergenic
TCCATAGGACCAGGTCTCAA+TGG 0.502603 1.3:+10087294 None:intergenic
CAATCTGAACCATGAGATCA+TGG 0.502886 1.3:+10087646 None:intergenic
GACTCGATTTATTGCCGGAA+AGG 0.519224 1.3:-10087322 MS.gene86318:CDS
CAATGACGTTCAAAGTTACC+TGG 0.520270 1.3:+10087225 None:intergenic
GTCCTAAAGTCGCCATCGGA+AGG 0.521014 1.3:+10087675 None:intergenic
TCAAGAACCAACGGGCTAGT+AGG 0.521826 1.3:-10087153 MS.gene86318:CDS
TGGAAGTGTTGTGGCTTCAA+AGG 0.524842 1.3:+10087803 None:intergenic
TACAAGAGCTGGAAGTGTTG+TGG 0.534283 1.3:+10087794 None:intergenic
GTATGGGCGTTGAATAAGAA+TGG 0.536944 1.3:-10087447 MS.gene86318:CDS
GACAGACATCCATGATCTCA+TGG 0.543777 1.3:-10087655 MS.gene86318:CDS
GGCTATGGAACTTCGTGTGG+CGG 0.546145 1.3:-10087006 MS.gene86318:CDS
TTCTTGATCCAATGGTGAGT+TGG 0.549706 1.3:+10087169 None:intergenic
ATCACTGTTCAGGTTCGCTG+AGG 0.550563 1.3:+10087083 None:intergenic
ATTTGCCACATCATCAGTGT+TGG 0.551734 1.3:-10089695 MS.gene86318:CDS
TCTGGTATTCAGGTTATGGT+TGG 0.552421 1.3:-10089526 MS.gene86318:CDS
CACTTCCAACACTGATGATG+TGG 0.559904 1.3:+10089690 None:intergenic
AATAAAAGGATAAATGTTCA+CGG 0.560448 1.3:+10087590 None:intergenic
AATGTGCAACCCTGAGTTGA+AGG 0.566126 1.3:+10086871 None:intergenic
CTTGGGCAAATGTGCTACTC+CGG 0.568572 1.3:+10087125 None:intergenic
TAATTGTTGGAGAGATCGGT+TGG 0.569155 1.3:-10087407 MS.gene86318:CDS
CACATCATCAGTGTTGGAAG+TGG 0.569256 1.3:-10089689 MS.gene86318:CDS
ACCATTGGATCAAGAACCAA+CGG 0.573644 1.3:-10087162 MS.gene86318:CDS
TAAATCGAGTCATGAAACCT+TGG 0.576604 1.3:+10087334 None:intergenic
GACAGAGATCCTTCAACTCA+GGG 0.576853 1.3:-10086880 MS.gene86318:CDS
GTCTGTCCTAAAGTCGCCAT+CGG 0.577196 1.3:+10087671 None:intergenic
TAACTATGATACTCTAGTAT+GGG 0.577250 1.3:-10087463 MS.gene86318:CDS
CCGTTGTAAGTTGACAAGAA+AGG 0.578866 1.3:+10087828 None:intergenic
CTAAACAAGTATGCATCCAT+AGG 0.581623 1.3:+10087279 None:intergenic
GACTGCACTAGTCTTGGCTA+TGG 0.584565 1.3:-10087021 MS.gene86318:CDS
CCTTTCTTGTCAACTTACAA+CGG 0.587520 1.3:-10087828 MS.gene86318:CDS
TCCTAAAGTCGCCATCGGAA+GGG 0.589717 1.3:+10087676 None:intergenic
AACGGGCTAGTAGGTGCTAC+CGG 0.592787 1.3:-10087144 MS.gene86318:CDS
GATACACATCAGCATTTGAA+GGG 0.593720 1.3:+10087715 None:intergenic
CTCGATTTATTGCCGGAAAG+GGG 0.594300 1.3:-10087320 MS.gene86318:CDS
CCATTGGATCAAGAACCAAC+GGG 0.594581 1.3:-10087161 MS.gene86318:CDS
CCTACAGACGGAGACAGAAA+CGG 0.595732 1.3:-10087384 MS.gene86318:CDS
TCACTGTTCAGGTTCGCTGA+GGG 0.597689 1.3:+10087084 None:intergenic
CTTGGCTATGGAACTTCGTG+TGG 0.608758 1.3:-10087009 MS.gene86318:CDS
AGTGATCCTTTAAACGACAA+CGG 0.610182 1.3:-10087513 MS.gene86318:CDS
TCATCAGTGTTGGAAGTGGG+TGG 0.618212 1.3:-10089685 MS.gene86318:CDS
TAGCACATTTGCCCAAGAAG+TGG 0.619134 1.3:-10087119 MS.gene86318:CDS
AAGTATGCATCCATAGGACC+AGG 0.624541 1.3:+10087285 None:intergenic
TCATCAGGTAACCCTTCCGA+TGG 0.628858 1.3:-10087687 MS.gene86318:CDS
GAGATCGGTTGGCCTACAGA+CGG 0.643611 1.3:-10087396 MS.gene86318:CDS
GGTAACTTTGAACGTCATTG+GGG 0.647034 1.3:-10087222 MS.gene86318:CDS
GGTAGCACCTACTAGCCCGT+TGG 0.654363 1.3:+10087146 None:intergenic
AAGGGGACTCCATTGAGACC+TGG 0.654522 1.3:-10087303 MS.gene86318:CDS
TGGCAAGCATAAGACACACT+CGG 0.661204 1.3:+10087051 None:intergenic
ACATCATCAGTGTTGGAAGT+GGG 0.662999 1.3:-10089688 MS.gene86318:CDS
AAGAGATTCGATTCTCCTCG+CGG 0.664201 1.3:+10086825 None:intergenic
TCCATTACCACCATCTACTG+TGG 0.670093 1.3:-10089629 MS.gene86318:CDS
GACAACAAAAGCATTCAACC+AGG 0.676173 1.3:-10087243 MS.gene86318:CDS
GGTGGAATTGGAGTGAACTG+GGG 0.692379 1.3:-10089667 MS.gene86318:CDS
TGTGGCGGTTTAGATATCCG+TGG 0.708567 1.3:-10086991 MS.gene86318:CDS
TCAGTGACAGTAGAAAGACC+GGG 0.731431 1.3:+10086901 None:intergenic
ACGCATATGAGATGTTACCA+CGG 0.737125 1.3:+10086974 None:intergenic
TGATCCAAACTGATTCCGCG+AGG 0.757114 1.3:-10086840 MS.gene86318:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTACATCAATATAAAA+AGG + chr1.3:10088438-10088457 None:intergenic 10.0%
!!! TTTTATATTGATGTAAAATA+CGG - chr1.3:10088438-10088457 MS.gene86318:intron 10.0%
!! AAATTAGACAAAATTTATGT+AGG - chr1.3:10087309-10087328 MS.gene86318:CDS 15.0%
!! AATTAGACAAAATTTATGTA+GGG - chr1.3:10087310-10087329 MS.gene86318:CDS 15.0%
!! TTTATATTGATGTAAAATAC+GGG - chr1.3:10088439-10088458 MS.gene86318:intron 15.0%
!!! AATTCAATTTTGAAGATTAA+TGG - chr1.3:10087839-10087858 MS.gene86318:CDS 15.0%
!!! ATTTCATGTAATTTTTTTCA+AGG - chr1.3:10088614-10088633 MS.gene86318:intron 15.0%
!!! GTAAAAAGTAAACTTTTTTA+TGG + chr1.3:10087244-10087263 None:intergenic 15.0%
!!! GTTAAATATGATTTTACTTA+GGG + chr1.3:10087509-10087528 None:intergenic 15.0%
!!! TATTTATTTATTCTGCATTT+TGG - chr1.3:10087172-10087191 MS.gene86318:CDS 15.0%
!! AAACAAATAGAAAAACAAAC+AGG + chr1.3:10087998-10088017 None:intergenic 20.0%
!! AATAAAAGGATAAATGTTCA+CGG + chr1.3:10088899-10088918 None:intergenic 20.0%
!! ATAGTATAAAGCAAATCATT+TGG + chr1.3:10086990-10087009 None:intergenic 20.0%
!! GTGAAAATGTTAAAAGATAA+TGG - chr1.3:10086879-10086898 MS.gene86318:CDS 20.0%
!! TCTACAAATCATAGAATATT+TGG - chr1.3:10088079-10088098 MS.gene86318:intron 20.0%
!! TCTTCATAAAGACTAATAAA+AGG + chr1.3:10088913-10088932 None:intergenic 20.0%
!! TTAATTAAAACATTGACAAC+AGG + chr1.3:10088259-10088278 None:intergenic 20.0%
!! TTACTTTACTTGTATATAGA+TGG + chr1.3:10087531-10087550 None:intergenic 20.0%
!!! AAATGATTTGCTTTATACTA+TGG - chr1.3:10086989-10087008 MS.gene86318:CDS 20.0%
!!! AATCACTATTTTTCTTTTGT+AGG + chr1.3:10087288-10087307 None:intergenic 20.0%
!!! ATCACTATTTTTCTTTTGTA+GGG + chr1.3:10087287-10087306 None:intergenic 20.0%
!!! ATCATATATTTTTCCTTAAC+AGG + chr1.3:10088324-10088343 None:intergenic 20.0%
!!! ATTTTTTTCAAGGTATATTG+CGG - chr1.3:10088624-10088643 MS.gene86318:intron 20.0%
!!! GATTTTTTCAACAACTTTTT+TGG - chr1.3:10088110-10088129 MS.gene86318:intron 20.0%
!!! GGTTAAATATGATTTTACTT+AGG + chr1.3:10087510-10087529 None:intergenic 20.0%
!!! GTCAATGTTTTAATTAAATG+AGG - chr1.3:10088262-10088281 MS.gene86318:intron 20.0%
!!! TTTTCATTTCTTAATCTGTT+GGG - chr1.3:10088503-10088522 MS.gene86318:intron 20.0%
!!! TTTTTCATTTCTTAATCTGT+TGG - chr1.3:10088502-10088521 MS.gene86318:intron 20.0%
! AGTCATTTGTTTAACAGTTA+AGG + chr1.3:10088542-10088561 None:intergenic 25.0%
! ATTTGTAGATGAAGAATCAA+GGG + chr1.3:10088068-10088087 None:intergenic 25.0%
! GATTAAATTTAGCTGCAAAA+GGG - chr1.3:10088184-10088203 MS.gene86318:intron 25.0%
! TAACTATGATACTCTAGTAT+GGG - chr1.3:10089023-10089042 MS.gene86318:intron 25.0%
! TGATTAAATTTAGCTGCAAA+AGG - chr1.3:10088183-10088202 MS.gene86318:intron 25.0%
!! AAGTATTTTATTGGAAGGAA+AGG - chr1.3:10087623-10087642 MS.gene86318:CDS 25.0%
!! AATATGATTTTACTTAGGGA+AGG + chr1.3:10087505-10087524 None:intergenic 25.0%
!! ACATCAAGTATTTTATTGGA+AGG - chr1.3:10087618-10087637 MS.gene86318:CDS 25.0%
!! AGTATTTTATTGGAAGGAAA+GGG - chr1.3:10087624-10087643 MS.gene86318:CDS 25.0%
!! ATATGATTTTACTTAGGGAA+GGG + chr1.3:10087504-10087523 None:intergenic 25.0%
!! ATATGTCTTCCTAACTTTTT+GGG - chr1.3:10088380-10088399 MS.gene86318:intron 25.0%
!! CAACACATCAAGTATTTTAT+TGG - chr1.3:10087614-10087633 MS.gene86318:CDS 25.0%
!! TGTGTTGAGTTTTTAATCTT+TGG - chr1.3:10087115-10087134 MS.gene86318:CDS 25.0%
!! TTCAAAGCATCAAGAATATT+TGG + chr1.3:10086936-10086955 None:intergenic 25.0%
!! TTTAGTAAGAAAGTTTTTGC+AGG - chr1.3:10087676-10087695 MS.gene86318:CDS 25.0%
!! TTTTTGCAGGAACTTTTTAT+AGG - chr1.3:10087689-10087708 MS.gene86318:CDS 25.0%
!!! AATAGTACTGTTTGCTTTTT+TGG - chr1.3:10089686-10089705 MS.gene86318:CDS 25.0%
AATCAGTTTGGATCATGATT+TGG + chr1.3:10089641-10089660 None:intergenic 30.0%
AGTGAAGACATATTTAACCA+AGG - chr1.3:10087370-10087389 MS.gene86318:CDS 30.0%
AGTTATAATGTCTGCCAAAA+AGG - chr1.3:10088036-10088055 MS.gene86318:intron 30.0%
CTAACTATGATACTCTAGTA+TGG - chr1.3:10089022-10089041 MS.gene86318:intron 30.0%
GACAAGTAATTTCCATACAT+CGG + chr1.3:10087908-10087927 None:intergenic 30.0%
GATTTGTAGATGAAGAATCA+AGG + chr1.3:10088069-10088088 None:intergenic 30.0%
GGTTATGATTGTTTCAAACT+AGG - chr1.3:10087193-10087212 MS.gene86318:CDS 30.0%
GTGAAGACATATTTAACCAA+GGG - chr1.3:10087371-10087390 MS.gene86318:CDS 30.0%
GTTGCATGCATAAAATCTAA+AGG - chr1.3:10088354-10088373 MS.gene86318:intron 30.0%
TTCAAAATACACCACTTCTT+GGG + chr1.3:10089381-10089400 None:intergenic 30.0%
TTTCAAAATACACCACTTCT+TGG + chr1.3:10089382-10089401 None:intergenic 30.0%
TTTGAAGAAATCTGGTATTC+AGG - chr1.3:10086950-10086969 MS.gene86318:CDS 30.0%
TTTGTAGATGAAGAATCAAG+GGG + chr1.3:10088067-10088086 None:intergenic 30.0%
! AGATTTTATGCATGCAACAA+AGG + chr1.3:10088352-10088371 None:intergenic 30.0%
! GATATGTCTTCCTAACTTTT+TGG - chr1.3:10088379-10088398 MS.gene86318:intron 30.0%
! TATGTCTTCCTAACTTTTTG+GGG - chr1.3:10088381-10088400 MS.gene86318:intron 30.0%
! TGTGTTTGAAATCATTGACT+AGG - chr1.3:10087962-10087981 MS.gene86318:intron 30.0%
! TTTTGGATATTTACAAGAGC+TGG + chr1.3:10088706-10088725 None:intergenic 30.0%
!! GATTGTTTTATTACGATGGA+CGG - chr1.3:10089286-10089305 MS.gene86318:intron 30.0%
AATTTCACACTGACCTGTTA+AGG - chr1.3:10088308-10088327 MS.gene86318:intron 35.0%
ACTAAGATACTTCCGATGTA+TGG - chr1.3:10087893-10087912 MS.gene86318:intron 35.0%
AGGTAACTTTGAACGTCATT+GGG - chr1.3:10089263-10089282 MS.gene86318:intron 35.0%
AGTATGTATTGCTGTGAGTT+TGG - chr1.3:10087781-10087800 MS.gene86318:CDS 35.0%
AGTGATCCTTTAAACGACAA+CGG - chr1.3:10088973-10088992 MS.gene86318:intron 35.0%
ATGTAAAATACGGGCAATTG+CGG - chr1.3:10088448-10088467 MS.gene86318:intron 35.0%
CAAAACGCTCTTATAAAAGC+TGG - chr1.3:10088721-10088740 MS.gene86318:intron 35.0%
CCTTTCTTGTCAACTTACAA+CGG - chr1.3:10088658-10088677 MS.gene86318:intron 35.0%
CTAAACAAGTATGCATCCAT+AGG + chr1.3:10089210-10089229 None:intergenic 35.0%
GAAATTACTTGTCGTTCCTT+TGG - chr1.3:10087915-10087934 MS.gene86318:intron 35.0%
GATACACATCAGCATTTGAA+GGG + chr1.3:10088774-10088793 None:intergenic 35.0%
GATTCCACTAAGTATTCCAA+AGG + chr1.3:10087934-10087953 None:intergenic 35.0%
GGATAAATGTTCACGGTAAA+TGG + chr1.3:10088892-10088911 None:intergenic 35.0%
TAAATCGAGTCATGAAACCT+TGG + chr1.3:10089155-10089174 None:intergenic 35.0%
TCTCTTGAACAATTCGTCTT+TGG + chr1.3:10087425-10087444 None:intergenic 35.0%
TGATACACATCAGCATTTGA+AGG + chr1.3:10088775-10088794 None:intergenic 35.0%
TGATTGTTTCAAACTAGGAG+AGG - chr1.3:10087198-10087217 MS.gene86318:CDS 35.0%
TGCGTTTAATAGCTACTATC+AGG - chr1.3:10089530-10089549 MS.gene86318:CDS 35.0%
TTCATGACTCGATTTATTGC+CGG - chr1.3:10089159-10089178 MS.gene86318:intron 35.0%
TTGAAAAATGCATAGTCGAC+TGG + chr1.3:10088946-10088965 None:intergenic 35.0%
! AACATTTTCACCACAGTAGA+TGG + chr1.3:10086870-10086889 None:intergenic 35.0%
! ATCTTTCCGTTGTCGTTTAA+AGG + chr1.3:10088982-10089001 None:intergenic 35.0%
! ATGTCTTCCTAACTTTTTGG+GGG - chr1.3:10088382-10088401 MS.gene86318:intron 35.0%
! GAAATCTGGTATTCAGGTTA+TGG - chr1.3:10086956-10086975 MS.gene86318:CDS 35.0%
! GCTCTTATAAAAGCTGGTTT+GGG - chr1.3:10088727-10088746 MS.gene86318:intron 35.0%
! TGCAGGAACTTTTTATAGGA+TGG - chr1.3:10087693-10087712 MS.gene86318:CDS 35.0%
!! CAACAGGTTGATATTTGTAC+AGG + chr1.3:10088243-10088262 None:intergenic 35.0%
!! CTTGATGCTTTGAAGAAATC+TGG - chr1.3:10086942-10086961 MS.gene86318:CDS 35.0%
!! GTCGACTATGCATTTTTCAA+CGG - chr1.3:10088946-10088965 MS.gene86318:intron 35.0%
!! TGGGGATTGTTTTATTACGA+TGG - chr1.3:10089282-10089301 MS.gene86318:intron 35.0%
!! TTTTCAAGGTATATTGCGGT+TGG - chr1.3:10088628-10088647 MS.gene86318:intron 35.0%
!!! CAGCTTTTATAAGAGCGTTT+TGG + chr1.3:10088723-10088742 None:intergenic 35.0%
ACCATTGGATCAAGAACCAA+CGG - chr1.3:10089324-10089343 MS.gene86318:intron 40.0%
ACCGATCTCTCCAACAATTA+TGG + chr1.3:10089079-10089098 None:intergenic 40.0%
ACGCATATGAGATGTTACCA+CGG + chr1.3:10089515-10089534 None:intergenic 40.0%
ACTCGATTTATTGCCGGAAA+GGG - chr1.3:10089165-10089184 MS.gene86318:intron 40.0%
ATTTGCCACATCATCAGTGT+TGG - chr1.3:10086791-10086810 MS.gene86318:CDS 40.0%
CAATCTGAACCATGAGATCA+TGG + chr1.3:10088843-10088862 None:intergenic 40.0%
CAATGACGTTCAAAGTTACC+TGG + chr1.3:10089264-10089283 None:intergenic 40.0%
CAGGTAACTTTGAACGTCAT+TGG - chr1.3:10089262-10089281 MS.gene86318:intron 40.0%
CCCATAATTGTTGGAGAGAT+CGG - chr1.3:10089075-10089094 MS.gene86318:intron 40.0%
CCGATCTCTCCAACAATTAT+GGG + chr1.3:10089078-10089097 None:intergenic 40.0%
CGCTCTTATAAAAGCTGGTT+TGG - chr1.3:10088726-10088745 MS.gene86318:intron 40.0%
CTACTATCAGGTGAATGATC+AGG - chr1.3:10089542-10089561 MS.gene86318:CDS 40.0%
GATATTTGTACAGGTGGACA+AGG + chr1.3:10088234-10088253 None:intergenic 40.0%
GCATAGTCGACTGGAAAATT+GGG + chr1.3:10088937-10088956 None:intergenic 40.0%
GCGTTGAATAAGAATGGCTT+TGG - chr1.3:10089045-10089064 MS.gene86318:intron 40.0%
GGAGAGGTGAGAAATTATAG+TGG - chr1.3:10087214-10087233 MS.gene86318:CDS 40.0%
GGTAACATGCCCATAATTGT+TGG - chr1.3:10089066-10089085 MS.gene86318:intron 40.0%
GGTAACTTTGAACGTCATTG+GGG - chr1.3:10089264-10089283 MS.gene86318:intron 40.0%
GTTTCAGCTCATGTTTCATC+TGG - chr1.3:10087053-10087072 MS.gene86318:CDS 40.0%
TAATTGTTGGAGAGATCGGT+TGG - chr1.3:10089079-10089098 MS.gene86318:intron 40.0%
TCGTCAAGCTGCTGAAAAAT+GGG - chr1.3:10087019-10087038 MS.gene86318:CDS 40.0%
TGCATAGTCGACTGGAAAAT+TGG + chr1.3:10088938-10088957 None:intergenic 40.0%
TGTAAAATACGGGCAATTGC+GGG - chr1.3:10088449-10088468 MS.gene86318:intron 40.0%
TTCTTGATCCAATGGTGAGT+TGG + chr1.3:10089320-10089339 None:intergenic 40.0%
! ACATCATCAGTGTTGGAAGT+GGG - chr1.3:10086798-10086817 MS.gene86318:CDS 40.0%
! ATTTTCACCACAGTAGATGG+TGG + chr1.3:10086867-10086886 None:intergenic 40.0%
! CCGTTGTAAGTTGACAAGAA+AGG + chr1.3:10088661-10088680 None:intergenic 40.0%
! TCTGGTATTCAGGTTATGGT+TGG - chr1.3:10086960-10086979 MS.gene86318:CDS 40.0%
!! CAGGTTGATATTTGTACAGG+TGG + chr1.3:10088240-10088259 None:intergenic 40.0%
!! CCAATGGTGAGTTGGTATTT+CGG + chr1.3:10089312-10089331 None:intergenic 40.0%
!! CGTTCCTTTGGAATACTTAG+TGG - chr1.3:10087927-10087946 MS.gene86318:intron 40.0%
!! GACAACAAAAGCATTCAACC+AGG - chr1.3:10089243-10089262 MS.gene86318:intron 40.0%
!! GTATGGGCGTTGAATAAGAA+TGG - chr1.3:10089039-10089058 MS.gene86318:intron 40.0%
AAGAGATTCGATTCTCCTCG+CGG + chr1.3:10089664-10089683 None:intergenic 45.0%
AAGGGCCCTTGTAATTTGGT+TGG + chr1.3:10087486-10087505 None:intergenic 45.0%
AAGTATGCATCCATAGGACC+AGG + chr1.3:10089204-10089223 None:intergenic 45.0%
CATCTGGTGGAGTAGATATC+AGG - chr1.3:10087069-10087088 MS.gene86318:CDS 45.0%
CCATTGGATCAAGAACCAAC+GGG - chr1.3:10089325-10089344 MS.gene86318:intron 45.0%
CCGAAATACCAACTCACCAT+TGG - chr1.3:10089309-10089328 MS.gene86318:intron 45.0%
CCTCTCTTCTGCATTTGACT+CGG - chr1.3:10087744-10087763 MS.gene86318:CDS 45.0%
CGAGTCAAATGCAGAAGAGA+GGG + chr1.3:10087746-10087765 None:intergenic 45.0%
CTCGATTTATTGCCGGAAAG+GGG - chr1.3:10089166-10089185 MS.gene86318:intron 45.0%
CTTGCTTGCACTGAATTCAG+TGG - chr1.3:10088153-10088172 MS.gene86318:intron 45.0%
GACAGACATCCATGATCTCA+TGG - chr1.3:10088831-10088850 MS.gene86318:intron 45.0%
GACAGAGATCCTTCAACTCA+GGG - chr1.3:10089606-10089625 MS.gene86318:CDS 45.0%
GACGGCCAACCAAATTACAA+GGG - chr1.3:10087478-10087497 MS.gene86318:CDS 45.0%
GACTCGATTTATTGCCGGAA+AGG - chr1.3:10089164-10089183 MS.gene86318:intron 45.0%
GAGTCAAATGCAGAAGAGAG+GGG + chr1.3:10087745-10087764 None:intergenic 45.0%
GATGTGTATCAGAGCTCATC+AGG - chr1.3:10088784-10088803 MS.gene86318:intron 45.0%
GTAAAATACGGGCAATTGCG+GGG - chr1.3:10088450-10088469 MS.gene86318:intron 45.0%
GTACAGGTGGACAAGGATTT+AGG + chr1.3:10088227-10088246 None:intergenic 45.0%
GTCAGTGACAGTAGAAAGAC+CGG + chr1.3:10089589-10089608 None:intergenic 45.0%
GTCGTCAAGCTGCTGAAAAA+TGG - chr1.3:10087018-10087037 MS.gene86318:CDS 45.0%
GTGCAACTTGATCACTGTTC+AGG + chr1.3:10089416-10089435 None:intergenic 45.0%
TAGCACATTTGCCCAAGAAG+TGG - chr1.3:10089367-10089386 MS.gene86318:intron 45.0%
TCAGCTCATGTTTCATCTGG+TGG - chr1.3:10087056-10087075 MS.gene86318:CDS 45.0%
TCAGTGACAGTAGAAAGACC+GGG + chr1.3:10089588-10089607 None:intergenic 45.0%
TCCATTACCACCATCTACTG+TGG - chr1.3:10086857-10086876 MS.gene86318:CDS 45.0%
TGACAGAGATCCTTCAACTC+AGG - chr1.3:10089605-10089624 MS.gene86318:CDS 45.0%
TGACGGCCAACCAAATTACA+AGG - chr1.3:10087477-10087496 MS.gene86318:CDS 45.0%
TGGCAAGCATAAGACACACT+CGG + chr1.3:10089438-10089457 None:intergenic 45.0%
TGGTGGAGTAGATATCAGGT+TGG - chr1.3:10087073-10087092 MS.gene86318:CDS 45.0%
TGTATTGCTGTGAGTTTGGC+AGG - chr1.3:10087785-10087804 MS.gene86318:CDS 45.0%
! AATGCAGACTGCACTAGTCT+TGG - chr1.3:10089459-10089478 MS.gene86318:CDS 45.0%
! AATGTGCAACCCTGAGTTGA+AGG + chr1.3:10089618-10089637 None:intergenic 45.0%
! ACCACAGTAGATGGTGGTAA+TGG + chr1.3:10086861-10086880 None:intergenic 45.0%
! CACATCATCAGTGTTGGAAG+TGG - chr1.3:10086797-10086816 MS.gene86318:CDS 45.0%
! CACTTCCAACACTGATGATG+TGG + chr1.3:10086799-10086818 None:intergenic 45.0%
! GACTAGTGCAGTCTGCATTT+TGG + chr1.3:10089458-10089477 None:intergenic 45.0%
!! ACTTCTAGAGCTCAATCCCT+TGG + chr1.3:10087390-10087409 None:intergenic 45.0%
!! ATCAAGGGGCATGTCCTTTT+TGG + chr1.3:10088053-10088072 None:intergenic 45.0%
!! CAGGTGAATGATCAGGTTGA+TGG - chr1.3:10089549-10089568 MS.gene86318:CDS 45.0%
!! TACAAGAGCTGGAAGTGTTG+TGG + chr1.3:10088695-10088714 None:intergenic 45.0%
!! TGGAAGTGTTGTGGCTTCAA+AGG + chr1.3:10088686-10088705 None:intergenic 45.0%
ACCTGAGTTCGATTCCTGGT+TGG - chr1.3:10087445-10087464 MS.gene86318:CDS 50.0%
AGGGAAGGGCCCTTGTAATT+TGG + chr1.3:10087490-10087509 None:intergenic 50.0%
CACTCAGCCCCCAAAAAGTT+AGG + chr1.3:10088392-10088411 None:intergenic 50.0%
CCGAGTCAAATGCAGAAGAG+AGG + chr1.3:10087747-10087766 None:intergenic 50.0%
CCTACAGACGGAGACAGAAA+CGG - chr1.3:10089102-10089121 MS.gene86318:intron 50.0%
CCTGAGTTCGATTCCTGGTT+GGG - chr1.3:10087446-10087465 MS.gene86318:CDS 50.0%
CTGGTTGGGACGATTCTTGA+CGG - chr1.3:10087460-10087479 MS.gene86318:CDS 50.0%
CTTGGCTATGGAACTTCGTG+TGG - chr1.3:10089477-10089496 MS.gene86318:CDS 50.0%
GAGAACCTGAGTTCGATTCC+TGG - chr1.3:10087441-10087460 MS.gene86318:CDS 50.0%
GATGGCGCTTGCAAATTTCC+CGG - chr1.3:10089567-10089586 MS.gene86318:CDS 50.0%
GGGTGGAATTGGAGTGAACT+GGG - chr1.3:10086818-10086837 MS.gene86318:CDS 50.0%
GGTGGAATTGGAGTGAACTG+GGG - chr1.3:10086819-10086838 MS.gene86318:CDS 50.0%
GTCTGTCCTAAAGTCGCCAT+CGG + chr1.3:10088818-10088837 None:intergenic 50.0%
GTGTTGGAAGTGGGTGGAAT+TGG - chr1.3:10086807-10086826 MS.gene86318:CDS 50.0%
TCATCAGGTAACCCTTCCGA+TGG - chr1.3:10088799-10088818 MS.gene86318:intron 50.0%
TCCATAGGACCAGGTCTCAA+TGG + chr1.3:10089195-10089214 None:intergenic 50.0%
TCCATTGAGACCTGGTCCTA+TGG - chr1.3:10089191-10089210 MS.gene86318:intron 50.0%
TCCTAAAGTCGCCATCGGAA+GGG + chr1.3:10088813-10088832 None:intergenic 50.0%
TCTCAATGGAGTCCCCTTTC+CGG + chr1.3:10089181-10089200 None:intergenic 50.0%
TGGGTGGAATTGGAGTGAAC+TGG - chr1.3:10086817-10086836 MS.gene86318:CDS 50.0%
TGTGGCGGTTTAGATATCCG+TGG - chr1.3:10089495-10089514 MS.gene86318:CDS 50.0%
TTCTCCTCGCGGAATCAGTT+TGG + chr1.3:10089653-10089672 None:intergenic 50.0%
! ATCACTGTTCAGGTTCGCTG+AGG + chr1.3:10089406-10089425 None:intergenic 50.0%
! CTTGGGCAAATGTGCTACTC+CGG + chr1.3:10089364-10089383 None:intergenic 50.0%
! TCACTGTTCAGGTTCGCTGA+GGG + chr1.3:10089405-10089424 None:intergenic 50.0%
! TCATCAGTGTTGGAAGTGGG+TGG - chr1.3:10086801-10086820 MS.gene86318:CDS 50.0%
! TGATCCAAACTGATTCCGCG+AGG - chr1.3:10089646-10089665 MS.gene86318:CDS 50.0%
!! CCCGTTGGTTCTTGATCCAA+TGG + chr1.3:10089328-10089347 None:intergenic 50.0%
!! CTCGCGCAACGTTTTAACCA+AGG - chr1.3:10089135-10089154 MS.gene86318:intron 50.0%
!! GACTGCACTAGTCTTGGCTA+TGG - chr1.3:10089465-10089484 MS.gene86318:CDS 50.0%
!! TCAAGAACCAACGGGCTAGT+AGG - chr1.3:10089333-10089352 MS.gene86318:intron 50.0%
AAGGGGACTCCATTGAGACC+TGG - chr1.3:10089183-10089202 MS.gene86318:intron 55.0%
ACCCTTCCGATGGCGACTTT+AGG - chr1.3:10088809-10088828 MS.gene86318:intron 55.0%
CCCAACCAGGAATCGAACTC+AGG + chr1.3:10087449-10087468 None:intergenic 55.0%
CCGTTTCTGTCTCCGTCTGT+AGG + chr1.3:10089105-10089124 None:intergenic 55.0%
CGTCAAGAATCGTCCCAACC+AGG + chr1.3:10087462-10087481 None:intergenic 55.0%
GGCTATGGAACTTCGTGTGG+CGG - chr1.3:10089480-10089499 MS.gene86318:CDS 55.0%
GTCCTAAAGTCGCCATCGGA+AGG + chr1.3:10088814-10088833 None:intergenic 55.0%
!! AACGGGCTAGTAGGTGCTAC+CGG - chr1.3:10089342-10089361 MS.gene86318:intron 55.0%
!! CTTTTTGGGGGCTGAGTGCA+TGG - chr1.3:10088394-10088413 MS.gene86318:intron 55.0%
!! GAGATCGGTTGGCCTACAGA+CGG - chr1.3:10089090-10089109 MS.gene86318:intron 55.0%
GGTAGCACCTACTAGCCCGT+TGG + chr1.3:10089343-10089362 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 10086783 10089725 10086783 ID=MS.gene86318
chr1.3 mRNA 10086783 10089725 10086783 ID=MS.gene86318.t1;Parent=MS.gene86318
chr1.3 exon 10089418 10089725 10089418 ID=MS.gene86318.t1.exon1;Parent=MS.gene86318.t1
chr1.3 CDS 10089418 10089725 10089418 ID=cds.MS.gene86318.t1;Parent=MS.gene86318.t1
chr1.3 exon 10086783 10087872 10086783 ID=MS.gene86318.t1.exon2;Parent=MS.gene86318.t1
chr1.3 CDS 10086783 10087872 10086783 ID=cds.MS.gene86318.t1;Parent=MS.gene86318.t1
Gene Sequence

>MS.gene86318

ATGATGTTATTTGCCACATCATCAGTGTTGGAAGTGGGTGGAATTGGAGTGAACTGGGGAACACAGTCAACACATCCATTACCACCATCTACTGTGGTGAAAATGTTAAAAGATAATGGTATTCAAAAAGTTAAGCTTTTTGATGCTGATCCAAATATTCTTGATGCTTTGAAGAAATCTGGTATTCAGGTTATGGTTGGTATACCAAATGATTTGCTTTATACTATGGCTAATAGTCGTCAAGCTGCTGAAAAATGGGTTGCTAAGAATGTTTCAGCTCATGTTTCATCTGGTGGAGTAGATATCAGGTTGGTAAACTCTTTTGATTCATTTGTGTTGAGTTTTTAATCTTTGGTTTAGATTTTAATTCTTGTATAGAGTGATTCTGTTATTTATTTATTCTGCATTTTGGTTATGATTGTTTCAAACTAGGAGAGGTGAGAAATTATAGTGGTTTACCATAAAAAAGTTTACTTTTTACATAAAATGAGTGTTTTTAGTCCCTACAAAAGAAAAATAGTGATTAAAATTAGACAAAATTTATGTAGGGACTAACTTGCAAATTAAAAAAAATTTATAGTAAGAATAGTGAAGACATATTTAACCAAGGGATTGAGCTCTAGAAGTTGATTAGTTTCGCCAAAGACGAATTGTTCAAGAGAACCTGAGTTCGATTCCTGGTTGGGACGATTCTTGACGGCCAACCAAATTACAAGGGCCCTTCCCTAAGTAAAATCATATTTAACCATCTATATACAAGTAAAGTAATCGTTTCTGCAGTTACAATCACATACACAGACACAAACACATTCTATGAATGTCAATAATCTTCAACACATCAAGTATTTTATTGGAAGGAAAGGGCAAATTTACATTTTACGCTGTTATAAGCATTTAGTAAGAAAGTTTTTGCAGGAACTTTTTATAGGATGGTTCATGTAAGATTTAAAATAGTTACTCCCCTCTCTTCTGCATTTGACTCGGTGAAAATATTGTTTAGTATGTATTGCTGTGAGTTTGGCAGGTTTGTTGCACTAAAACTGTTTTATCTGATTCAATTCAATTTTGAAGATTAATGGAATTGAACAATTTTAGAAATTTAGCATTGCAACTAAGATACTTCCGATGTATGGAAATTACTTGTCGTTCCTTTGGAATACTTAGTGGAATCAATCGCAATGTGTTTGAAATCATTGACTAGGATTTATATATCCTGTTTGTTTTTCTATTTGTTTTTTCATGAATGACATTATAGTTATAATGTCTGCCAAAAAGGACATGCCCCTTGATTCTTCATCTACAAATCATAGAATATTTGGCAACATAAGATTTTTTCAACAACTTTTTTGGCAACATATTATCTTTGCATTCTTGCTTGCACTGAATTCAGTGGATTAAATTGATTAAATTTAGCTGCAAAAGGGTGAAATTCAGATATGAGCCTAAATCCTTGTCCACCTGTACAAATATCAACCTGTTGTCAATGTTTTAATTAAATGAGGACTCAAAGTAAGACATATTATCAAATTTCACACTGACCTGTTAAGGAAAAATATATGATTATTTCCTTTGTTGCATGCATAAAATCTAAAGGTTGATATGTCTTCCTAACTTTTTGGGGGCTGAGTGCATGGTTTTTAAGTTACTGTAAACCTTTTTATATTGATGTAAAATACGGGCAATTGCGGGGTCTTATGTGTTGTGTGTGCTTTTGCTATGTTTTTCATTTCTTAATCTGTTGGGACTGATGACATTTCCTTAACTGTTAAACAAATGACTATTCATATGTATTTCAATGTTGCAGTTGTCTATATTTTTCTTTAACTTTTTTATTTCATGTAATTTTTTTCAAGGTATATTGCGGTTGGAAATGAACCTTTCTTGTCAACTTACAACGGTACCTTTGAAGCCACAACACTTCCAGCTCTTGTAAATATCCAAAACGCTCTTATAAAAGCTGGTTTGGGAAGTTCAATCAAAGTCACTGTCCCTTCAAATGCTGATGTGTATCAGAGCTCATCAGGTAACCCTTCCGATGGCGACTTTAGGACAGACATCCATGATCTCATGGTTCAGATTGTCAAGTTTTTGAGTCAGAACAATGCTCCATTTACCGTGAACATTTATCCTTTTATTAGTCTTTATGAAGATCCCAATTTTCCAGTCGACTATGCATTTTTCAACGGTTTTAGTGATCCTTTAAACGACAACGGAAAGATCTACGACAATGTCTTTGATGCTAACTATGATACTCTAGTATGGGCGTTGAATAAGAATGGCTTTGGTAACATGCCCATAATTGTTGGAGAGATCGGTTGGCCTACAGACGGAGACAGAAACGGAAATCTTCAACTCGCGCAACGTTTTAACCAAGGTTTCATGACTCGATTTATTGCCGGAAAGGGGACTCCATTGAGACCTGGTCCTATGGATGCATACTTGTTTAGTTTAGTAGATGAAGACAACAAAAGCATTCAACCAGGTAACTTTGAACGTCATTGGGGATTGTTTTATTACGATGGACGGCCGAAATACCAACTCACCATTGGATCAAGAACCAACGGGCTAGTAGGTGCTACCGGAGTAGCACATTTGCCCAAGAAGTGGTGTATTTTGAAACCCTCAGCGAACCTGAACAGTGATCAAGTTGCACCGAGTGTGTCTTATGCTTGCCAAAATGCAGACTGCACTAGTCTTGGCTATGGAACTTCGTGTGGCGGTTTAGATATCCGTGGTAACATCTCATATGCGTTTAATAGCTACTATCAGGTGAATGATCAGGTTGATGGCGCTTGCAAATTTCCCGGTCTTTCTACTGTCACTGACAGAGATCCTTCAACTCAGGGTTGCACATTCCAAATCATGATCCAAACTGATTCCGCGAGGAGAATCGAATCTCTTAGAATAGTACTGTTTGCTTTTTTGGTTGTTGCTTTTTTGTAA
Protein sequence

>MS.gene86318.t1

MMLFATSSVLEVGGIGVNWGTQSTHPLPPSTVVKMLKDNGIQKVKLFDADPNILDALKKSGIQVMVGIPNDLLYTMANSRQAAEKWVAKNVSAHVSSGGVDIRYIAVGNEPFLSTYNGTFEATTLPALVNIQNALIKAGLGSSIKVTVPSNADVYQSSSGNPSDGDFRTDIHDLMVQIVKFLSQNNAPFTVNIYPFISLYEDPNFPVDYAFFNGFSDPLNDNGKIYDNVFDANYDTLVWALNKNGFGNMPIIVGEIGWPTDGDRNGNLQLAQRFNQGFMTRFIAGKGTPLRPGPMDAYLFSLVDEDNKSIQPGNFERHWGLFYYDGRPKYQLTIGSRTNGLVGATGVAHLPKKWCILKPSANLNSDQVAPSVSYACQNADCTSLGYGTSCGGLDIRGNISYAFNSYYQVNDQVDGACKFPGLSTVTDRDPSTQGCTFQIMIQTDSARRIESLRIVLFAFLVVAFL