AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86338

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86338.t1 MTR_2g102730 96.503 429 9 1 1 429 1 423 0.0 859
MS.gene86338.t1 MTR_4g011530 71.078 408 95 6 23 415 45 444 0.0 573
MS.gene86338.t1 MTR_2g009340 36.117 443 222 6 10 395 219 657 8.70e-84 270
MS.gene86338.t1 MTR_4g093850 38.391 435 214 10 7 390 215 646 2.74e-83 269
MS.gene86338.t1 MTR_4g022330 38.814 371 192 7 24 370 211 570 5.93e-69 230
MS.gene86338.t1 MTR_5g032780 40.256 313 146 6 24 299 211 519 5.35e-67 225
MS.gene86338.t1 MTR_7g073980 37.565 386 209 10 24 390 208 580 9.48e-65 219
MS.gene86338.t1 MTR_2g018750 33.249 394 231 6 24 398 214 594 1.12e-64 218
MS.gene86338.t1 MTR_8g090220 37.884 293 159 5 24 294 215 506 6.85e-63 211
MS.gene86338.t1 MTR_8g090220 37.097 310 161 6 24 300 215 523 3.28e-62 212
MS.gene86338.t1 MTR_4g134160 76.821 151 18 4 263 410 31 167 1.28e-60 194
MS.gene86338.t1 MTR_1g088885 33.019 424 216 10 24 402 210 610 1.32e-60 207
MS.gene86338.t1 MTR_7g108290 35.897 390 194 10 24 375 213 584 1.81e-60 208
MS.gene86338.t1 MTR_1g106430 34.873 433 209 14 24 413 213 615 1.03e-59 205
MS.gene86338.t1 MTR_1g064360 33.062 369 198 7 24 362 190 539 3.27e-59 202
MS.gene86338.t1 MTR_1g094850 32.300 387 216 7 24 386 212 576 3.36e-57 198
MS.gene86338.t1 MTR_8g078900 39.735 302 149 5 24 303 187 477 5.05e-56 195
MS.gene86338.t1 MTR_8g078900 39.735 302 149 5 24 303 197 487 5.11e-56 196
MS.gene86338.t1 MTR_1g014070 39.796 294 139 7 24 298 213 487 5.31e-56 195
MS.gene86338.t1 MTR_5g015790 39.519 291 142 4 24 299 212 483 6.50e-56 195
MS.gene86338.t1 MTR_8g078900 39.735 302 149 5 24 303 227 517 8.19e-56 196
MS.gene86338.t1 MTR_5g016060 35.191 341 188 6 24 346 105 430 2.35e-55 191
MS.gene86338.t1 MTR_5g016060 35.191 341 188 6 24 346 219 544 1.11e-54 192
MS.gene86338.t1 MTR_4g095430 35.433 381 199 9 24 374 195 558 4.66e-54 189
MS.gene86338.t1 MTR_4g093850 39.100 289 136 6 7 258 215 500 1.37e-52 184
MS.gene86338.t1 MTR_3g062540 31.683 404 230 10 24 410 141 515 2.82e-52 183
MS.gene86338.t1 MTR_3g062540 31.683 404 230 10 24 410 183 557 5.95e-52 183
MS.gene86338.t1 MTR_4g134210 35.314 303 177 4 6 299 183 475 2.93e-51 182
MS.gene86338.t1 MTR_2g084595 35.862 290 169 4 24 300 212 497 8.58e-51 181
MS.gene86338.t1 MTR_1g082690 28.612 353 208 8 13 355 210 528 6.79e-47 169
MS.gene86338.t1 MTR_8g069330 34.405 311 144 6 24 299 199 484 2.63e-46 168
MS.gene86338.t1 MTR_5g075640 31.034 290 185 3 24 300 141 428 1.69e-41 154
MS.gene86338.t1 MTR_5g075640 31.034 290 185 3 24 300 184 471 3.06e-41 154
MS.gene86338.t1 MTR_2g027410 33.882 304 170 5 6 299 111 393 4.96e-40 150
MS.gene86338.t1 MTR_2g027410 35.315 286 154 5 24 299 201 465 1.33e-39 149
MS.gene86338.t1 MTR_2g048390 35.950 242 134 4 24 248 215 452 1.75e-39 147
MS.gene86338.t1 MTR_4g045767 32.281 285 172 5 112 378 257 538 6.08e-35 136
MS.gene86338.t1 MTR_8g078900 34.008 247 131 4 24 248 187 423 3.25e-32 127
MS.gene86338.t1 MTR_8g078900 34.008 247 131 4 24 248 227 463 6.80e-32 126
MS.gene86338.t1 MTR_4g074190 36.082 194 122 1 107 300 230 421 2.13e-31 125
MS.gene86338.t1 MTR_4g074190 36.082 194 122 1 107 300 276 467 2.74e-31 125
MS.gene86338.t1 MTR_2g084595 32.218 239 145 4 24 249 212 446 9.29e-31 123
MS.gene86338.t1 MTR_1g035720 33.508 191 122 2 107 297 272 457 7.67e-29 119
MS.gene86338.t1 MTR_2g010890 27.338 278 175 7 24 300 122 373 1.18e-19 91.3
MS.gene86338.t1 MTR_4g074190 34.722 144 92 1 107 250 276 417 3.63e-19 89.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86338.t1 AT3G15570 59.560 455 155 8 1 429 1 452 0.0 520
MS.gene86338.t1 AT1G52770 58.409 440 160 8 12 429 16 454 1.80e-176 501
MS.gene86338.t1 AT5G64330 33.758 471 204 8 24 390 252 718 1.73e-76 253
MS.gene86338.t1 AT5G64330 38.202 356 142 4 24 301 193 548 6.69e-73 240
MS.gene86338.t1 AT5G64330 38.202 356 142 4 24 301 252 607 2.74e-72 239
MS.gene86338.t1 AT5G03250 36.198 384 196 9 24 383 107 465 3.33e-67 222
MS.gene86338.t1 AT5G03250 36.198 384 196 9 24 383 219 577 3.90e-66 222
MS.gene86338.t1 AT1G30440 33.854 384 213 6 24 372 216 593 3.02e-63 216
MS.gene86338.t1 AT5G13600 35.068 365 210 7 24 374 210 561 1.27e-62 213
MS.gene86338.t1 AT3G08660 33.791 364 188 6 24 363 138 472 3.13e-59 202
MS.gene86338.t1 AT5G66560 40.506 316 161 7 13 317 208 507 4.57e-59 204
MS.gene86338.t1 AT5G66560 41.311 305 154 6 24 317 227 517 4.71e-59 204
MS.gene86338.t1 AT5G66560 40.506 316 161 7 13 317 247 546 6.80e-59 204
MS.gene86338.t1 AT3G08660 33.791 364 188 6 24 363 198 532 1.20e-58 202
MS.gene86338.t1 AT5G48800 35.825 388 194 8 24 382 221 582 2.61e-58 201
MS.gene86338.t1 AT5G67385 37.801 291 157 5 24 304 158 434 3.98e-57 197
MS.gene86338.t1 AT5G67385 37.801 291 157 5 24 304 158 434 3.98e-57 197
MS.gene86338.t1 AT5G67385 37.801 291 157 5 24 304 158 434 3.98e-57 197
MS.gene86338.t1 AT5G67385 37.801 291 157 5 24 304 158 434 3.98e-57 197
MS.gene86338.t1 AT1G03010 33.486 436 222 13 24 405 180 601 5.23e-57 198
MS.gene86338.t1 AT5G67385 37.801 291 157 5 24 304 203 479 9.42e-57 197
MS.gene86338.t1 AT1G03010 32.719 434 228 12 24 405 207 628 9.60e-57 197
MS.gene86338.t1 AT1G03010 33.486 436 222 13 24 405 206 627 1.13e-56 197
MS.gene86338.t1 AT2G47860 33.582 402 200 9 24 380 90 469 4.65e-56 193
MS.gene86338.t1 AT2G47860 33.582 402 200 9 24 380 90 469 4.65e-56 193
MS.gene86338.t1 AT2G30520 35.756 344 206 3 24 357 141 479 5.42e-56 194
MS.gene86338.t1 AT2G30520 35.756 344 206 3 24 357 141 479 5.42e-56 194
MS.gene86338.t1 AT2G30520 35.756 344 206 3 24 357 189 527 1.04e-55 194
MS.gene86338.t1 AT2G47860 33.582 402 200 9 24 380 208 587 2.22e-55 194
MS.gene86338.t1 AT2G47860 33.582 402 200 9 24 380 231 610 3.23e-55 194
MS.gene86338.t1 AT5G10250 32.800 375 224 7 23 376 212 579 6.29e-54 190
MS.gene86338.t1 AT3G26490 36.538 312 138 5 24 299 220 507 6.99e-54 189
MS.gene86338.t1 AT3G26490 36.538 312 138 5 24 299 220 507 6.99e-54 189
MS.gene86338.t1 AT3G26490 36.538 312 138 5 24 299 220 507 6.99e-54 189
MS.gene86338.t1 AT4G31820 37.582 306 145 7 24 316 159 431 9.38e-54 187
MS.gene86338.t1 AT4G31820 37.582 306 145 7 24 316 212 484 3.16e-53 187
MS.gene86338.t1 AT5G10250 32.603 365 223 7 23 376 212 564 1.66e-52 186
MS.gene86338.t1 AT2G14820 33.155 374 196 7 24 365 209 560 1.28e-51 184
MS.gene86338.t1 AT2G14820 33.155 374 196 7 24 365 209 560 1.28e-51 184
MS.gene86338.t1 AT1G67900 35.152 330 172 3 7 299 185 509 3.87e-51 182
MS.gene86338.t1 AT1G67900 35.152 330 172 3 7 299 185 509 3.87e-51 182
MS.gene86338.t1 AT1G67900 35.152 330 172 3 7 299 185 509 3.87e-51 182
MS.gene86338.t1 AT1G67900 35.152 330 172 3 7 299 185 509 3.87e-51 182
MS.gene86338.t1 AT1G67900 35.152 330 172 3 7 299 185 509 3.87e-51 182
MS.gene86338.t1 AT1G67900 35.152 330 172 3 7 299 185 509 3.87e-51 182
MS.gene86338.t1 AT3G44820 31.818 374 220 6 24 365 213 583 4.20e-51 182
MS.gene86338.t1 AT3G08570 31.915 376 198 8 24 365 212 563 9.10e-51 181
MS.gene86338.t1 AT5G47800 34.211 304 180 4 4 297 181 474 7.82e-49 175
MS.gene86338.t1 AT5G47800 34.211 304 180 4 4 297 181 474 7.82e-49 175
MS.gene86338.t1 AT5G47800 34.211 304 180 4 4 297 181 474 7.82e-49 175
MS.gene86338.t1 AT5G47800 34.211 304 180 4 4 297 294 587 4.01e-48 174
MS.gene86338.t1 AT4G37590 36.525 282 149 5 24 303 216 469 8.53e-48 172
MS.gene86338.t1 AT3G50840 33.791 364 223 5 24 387 160 505 5.40e-47 169
MS.gene86338.t1 AT3G50840 33.791 364 223 5 24 387 160 505 5.40e-47 169
MS.gene86338.t1 AT3G50840 33.791 364 223 5 24 387 196 541 9.89e-47 169
MS.gene86338.t1 AT2G23050 35.842 279 143 6 24 301 93 336 1.61e-45 161
MS.gene86338.t1 AT5G67440 36.140 285 158 5 24 299 170 439 2.17e-45 165
MS.gene86338.t1 AT5G67440 36.140 285 158 5 24 299 207 476 4.15e-45 165
MS.gene86338.t1 AT5G67440 36.140 285 158 5 24 299 207 476 4.15e-45 165
MS.gene86338.t1 AT2G23050 35.842 279 143 6 24 301 209 452 9.86e-45 162
MS.gene86338.t1 AT3G49970 32.197 264 156 6 24 284 151 394 1.37e-38 146
MS.gene86338.t1 AT3G49970 32.197 264 156 6 24 284 151 394 1.37e-38 146
MS.gene86338.t1 AT5G66560 36.290 248 133 5 13 249 247 480 3.67e-35 136
MS.gene86338.t1 AT2G47860 35.659 258 111 6 24 248 208 443 1.30e-33 131
MS.gene86338.t1 AT2G47860 35.659 258 111 6 24 248 231 466 1.52e-33 131
MS.gene86338.t1 AT3G26490 33.206 262 115 5 24 249 220 457 2.05e-33 131
MS.gene86338.t1 AT3G08660 31.727 249 131 4 24 256 198 423 2.42e-31 124
MS.gene86338.t1 AT3G19850 29.603 277 170 3 24 297 209 463 8.71e-30 122
MS.gene86338.t1 AT3G03510 32.558 258 146 6 51 304 133 366 4.59e-28 115
MS.gene86338.t1 AT3G22104 36.082 194 118 3 92 284 157 345 7.61e-28 114
MS.gene86338.t1 AT3G22104 36.082 194 118 3 92 284 157 345 7.61e-28 114
MS.gene86338.t1 AT3G22104 36.082 194 118 3 92 284 157 345 7.61e-28 114
MS.gene86338.t1 AT3G22104 36.082 194 118 3 92 284 232 420 9.75e-28 115
MS.gene86338.t1 AT1G50280 32.275 189 126 1 107 293 239 427 3.42e-25 107
MS.gene86338.t1 AT1G50280 32.275 189 126 1 107 293 272 460 4.25e-25 107
MS.gene86338.t1 AT5G17580 29.545 264 158 4 43 300 217 458 1.47e-22 100
MS.gene86338.t1 AT5G17580 29.545 264 158 4 43 300 202 443 1.93e-22 100
MS.gene86338.t1 AT5G48130 24.534 322 196 10 24 334 219 504 9.01e-19 89.4

Find 80 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGTTTGGACTTGTTTC+TGG 0.094629 2.3:-1923485 None:intergenic
CATGATGCTGTTACACTTTC+AGG 0.126707 2.3:-1923722 None:intergenic
GATGAAGCTGAAACTGAATT+TGG 0.156976 2.3:+1925706 MS.gene86338:CDS
TCTCAGTTTGTTGCACTTGC+TGG 0.262022 2.3:+1924115 MS.gene86338:CDS
TCATACTTGTGGGACCCTTT+TGG 0.268326 2.3:+1923949 MS.gene86338:CDS
AACTGAAGGAAGACGTTTAC+AGG 0.316073 2.3:+1925557 MS.gene86338:CDS
TTACCTTGCTGAAGCTGGTT+TGG 0.320602 2.3:+1924075 MS.gene86338:CDS
AACTCAACCGTCACGTTGAT+TGG 0.333646 2.3:+1925422 MS.gene86338:CDS
TCCAAAGTTACTTGTTGGTT+TGG 0.335510 2.3:-1923497 None:intergenic
CTTTCAAACATCAAACAAAA+AGG 0.338570 2.3:+1923578 MS.gene86338:CDS
TGGATGTTGAGCTTGTTATT+AGG 0.354204 2.3:+1923969 MS.gene86338:CDS
CTTTCTTGCTTCGACACGCT+TGG 0.365749 2.3:-1925277 None:intergenic
GGTGTTCGTTCCGATTTAAT+AGG 0.371945 2.3:+1923599 MS.gene86338:CDS
TAACTTTGGAATATTGTTGC+TGG 0.379039 2.3:+1923510 MS.gene86338:CDS
ATGAAGCTGAAACTGAATTT+GGG 0.381070 2.3:+1925707 MS.gene86338:CDS
AACTGGTGCTGCTTTGGTTA+AGG 0.381744 2.3:+1924033 MS.gene86338:CDS
AGAAGAAAAGTTTCTTCAAA+TGG 0.382710 2.3:+1925632 MS.gene86338:CDS
AATTTGAATTTGAAGTTTCA+AGG 0.404146 2.3:-1923661 None:intergenic
AATATTCCAAAGTTACTTGT+TGG 0.406337 2.3:-1923502 None:intergenic
AGTTAAAACTGGTGCTGCTT+TGG 0.406920 2.3:+1924027 MS.gene86338:CDS
CGAAATGAACACACAACAAA+TGG 0.410203 2.3:+1925525 MS.gene86338:CDS
GCTGAAACTGAATTTGGGTT+TGG 0.417674 2.3:+1925712 MS.gene86338:CDS
TTGTTATTAGGTTGGTTAAG+AGG 0.429280 2.3:+1923981 MS.gene86338:CDS
GAATTTGAAGTTTCAAGGTC+AGG 0.429853 2.3:-1923656 None:intergenic
CATGGAATGGAATCTTTGTC+TGG 0.430911 2.3:-1923791 None:intergenic
GATGATGGAACCTATTAAAT+CGG 0.436313 2.3:-1923609 None:intergenic
CAGCATCATGGATGAAGAAA+AGG 0.445106 2.3:+1923735 MS.gene86338:CDS
GATCATGATGGAGTTAAAAC+TGG 0.446409 2.3:+1924016 MS.gene86338:CDS
TTTGGTTAAGGTTGCTAAGT+TGG 0.446507 2.3:+1924045 MS.gene86338:CDS
ACGGTTGAGTTTCGTTTGTT+CGG 0.447010 2.3:-1925410 None:intergenic
TCCAAACCAACAAGTAACTT+TGG 0.448402 2.3:+1923496 MS.gene86338:CDS
GCTGCATGAAGTGAGGCTTC+TGG 0.449394 2.3:-1925340 None:intergenic
ATCTTGAGAATCGAATTTCA+TGG 0.451747 2.3:+1923879 MS.gene86338:CDS
GATTGTTACCTTGCTGAAGC+TGG 0.451910 2.3:+1924070 MS.gene86338:CDS
CTTCTTTCAGTCATACTTGT+GGG 0.454989 2.3:+1923939 MS.gene86338:CDS
TCAGTAGCTCTTGCATGGTT+AGG 0.455773 2.3:-1924145 None:intergenic
TTCAAGGTCAGGGAGCCATT+TGG 0.459356 2.3:-1923645 None:intergenic
TAACAAGCTCAACATCCAAA+AGG 0.463547 2.3:-1923964 None:intergenic
TGTTGAGCTTGTTATTAGGT+TGG 0.469392 2.3:+1923973 MS.gene86338:CDS
GTGCTTCTTTCAGTAGCTTG+AGG 0.496152 2.3:+1925446 MS.gene86338:CDS
CAATGTCATGCAAGCACAGA+TGG 0.500225 2.3:+1925594 MS.gene86338:CDS
GTTTGGAAGGCAAACACCTA+TGG 0.502755 2.3:+1925729 MS.gene86338:CDS
CCTTCTTAAAACAGCAAACA+TGG 0.504569 2.3:+1923829 MS.gene86338:CDS
CTAGTACTGTGGTCAAAAGT+AGG 0.505830 2.3:+1925761 MS.gene86338:CDS
AACAAGCTCAACATCCAAAA+GGG 0.506792 2.3:-1923963 None:intergenic
AATTTGAAGTTTCAAGGTCA+GGG 0.510124 2.3:-1923655 None:intergenic
CATGCAAGAGCTACTGATGA+TGG 0.510855 2.3:+1924151 MS.gene86338:CDS
AAACTGAATTTGGGTTTGGA+AGG 0.516582 2.3:+1925716 MS.gene86338:CDS
TTGAGGAGTCCAAGTGGATA+CGG 0.521600 2.3:+1925463 MS.gene86338:CDS
AACACACCACCGTATCCACT+TGG 0.522164 2.3:-1925472 None:intergenic
TGGTGGAAGAACACTGACTA+AGG 0.524858 2.3:-1923771 None:intergenic
TATTAAAAGCATCGGAGAGA+TGG 0.526919 2.3:+1925669 MS.gene86338:CDS
TACTTTAAGATAAGTGTCAA+TGG 0.534706 2.3:-1924185 None:intergenic
GGAAGCATAGTGAGTGATGA+TGG 0.534975 2.3:-1923624 None:intergenic
CTGAAAGTGTAACAGCATCA+TGG 0.536672 2.3:+1923723 MS.gene86338:CDS
AAGGAGGAAGTTGCATGGAA+TGG 0.540539 2.3:-1923804 None:intergenic
CGGTGGTGTGTTGGACCCAC+AGG 0.552148 2.3:+1925483 MS.gene86338:CDS
TGTGTAGGTTGATTGATAGC+AGG 0.557107 2.3:+1925308 MS.gene86338:CDS
AAAGTAGGACTCCTCCTAAG+TGG 0.563062 2.3:+1925776 MS.gene86338:CDS
TCACTCACTATGCTTCCAAA+TGG 0.563233 2.3:+1923630 MS.gene86338:CDS
GCAAGCACAGATGGACAAGA+TGG 0.567785 2.3:+1925603 MS.gene86338:CDS
TGAAATTCGATTCTCAAGAT+CGG 0.569341 2.3:-1923876 None:intergenic
CCTTCTTTCAGTCATACTTG+TGG 0.572660 2.3:+1923938 MS.gene86338:CDS
CATCATCAGTAGCTCTTGCA+TGG 0.574138 2.3:-1924150 None:intergenic
AGATGCATGCATCCTTGACA+TGG 0.577074 2.3:+1923538 MS.gene86338:CDS
TTAGAAAGACACCGTGCCTG+TGG 0.583694 2.3:-1925499 None:intergenic
GGACATGAAAACTAGTACTG+TGG 0.589920 2.3:+1925750 MS.gene86338:CDS
TAATGAAGCAATCCATGTCA+AGG 0.592917 2.3:-1923550 None:intergenic
ACTTGAATAACAGCTCTCAC+AGG 0.608705 2.3:-1925379 None:intergenic
CCACAAGTATGACTGAAAGA+AGG 0.608970 2.3:-1923938 None:intergenic
AAGTGGATACGGTGGTGTGT+TGG 0.620100 2.3:+1925474 MS.gene86338:CDS
TAGAAAGACACCGTGCCTGT+GGG 0.630868 2.3:-1925498 None:intergenic
TTCATGCAGCTCAAAACGAA+CGG 0.639540 2.3:+1925353 MS.gene86338:CDS
AGGCGAAGGAGGAAGTTGCA+TGG 0.639865 2.3:-1923809 None:intergenic
GGAATGGAATCTTTGTCTGG+TGG 0.645080 2.3:-1923788 None:intergenic
CATCCAAACCAGCTTCAGCA+AGG 0.652544 2.3:-1924078 None:intergenic
GTGTGTTGGACCCACAGGCA+CGG 0.663649 2.3:+1925488 MS.gene86338:CDS
AAGCACTCCAATCAACGTGA+CGG 0.680767 2.3:-1925429 None:intergenic
AGTAGCTTGAGGAGTCCAAG+TGG 0.684181 2.3:+1925457 MS.gene86338:CDS
AGGAGTCCAAGTGGATACGG+TGG 0.701403 2.3:+1925466 MS.gene86338:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATATACATATCTATA+AGG - chr2.3:1925008-1925027 None:intergenic 10.0%
!!! TATTTATTTATTTTTGCAAT+AGG + chr2.3:1925249-1925268 MS.gene86338:intron 10.0%
!! AATATACATATCTATAAGGA+AGG - chr2.3:1925004-1925023 None:intergenic 20.0%
!! AGAAAGAAATAAGTATTAAG+AGG + chr2.3:1924356-1924375 MS.gene86338:intron 20.0%
!! TAATTCTTTATAAAGATCTC+AGG + chr2.3:1924240-1924259 MS.gene86338:intron 20.0%
!!! AAGAAAATTAATGTGCTTTT+TGG - chr2.3:1925039-1925058 None:intergenic 20.0%
!!! AATTTGAATTTGAAGTTTCA+AGG - chr2.3:1923664-1923683 None:intergenic 20.0%
!!! TATACTTGTAAAGTCAATTT+TGG - chr2.3:1924724-1924743 None:intergenic 20.0%
!!! TTTTTGTTTGATGTTTGAAA+GGG - chr2.3:1923579-1923598 None:intergenic 20.0%
! AAGAAATAAGTATTAAGAGG+AGG + chr2.3:1924359-1924378 MS.gene86338:intron 25.0%
! AATATTCCAAAGTTACTTGT+TGG - chr2.3:1923505-1923524 None:intergenic 25.0%
! AATGGAGATAAAAAAACTGA+AGG + chr2.3:1925543-1925562 MS.gene86338:CDS 25.0%
! ACAAAATTCCAAATCTAGAT+AGG + chr2.3:1924803-1924822 MS.gene86338:intron 25.0%
! AGAAGAAAAGTTTCTTCAAA+TGG + chr2.3:1925632-1925651 MS.gene86338:CDS 25.0%
! ATTCAAGTAACATACCATTA+AGG + chr2.3:1925063-1925082 MS.gene86338:intron 25.0%
! CTTTCAAACATCAAACAAAA+AGG + chr2.3:1923578-1923597 MS.gene86338:CDS 25.0%
!!! ATTGTTTGTTTTTCATCTGA+AGG + chr2.3:1924757-1924776 MS.gene86338:intron 25.0%
!!! CTTGCTTTTATTAAAAGCAT+CGG + chr2.3:1925661-1925680 MS.gene86338:CDS 25.0%
!!! CTTTTTGTTTGATGTTTGAA+AGG - chr2.3:1923580-1923599 None:intergenic 25.0%
!!! TACTTTAAGATAAGTGTCAA+TGG - chr2.3:1924188-1924207 None:intergenic 25.0%
!!! TATCTAGATTTGGAATTTTG+TGG - chr2.3:1924804-1924823 None:intergenic 25.0%
AAGAAAGAAAAAGTCTGTGT+AGG + chr2.3:1925293-1925312 MS.gene86338:CDS 30.0%
AAGTAACATACCATTAAGGT+TGG + chr2.3:1925067-1925086 MS.gene86338:intron 30.0%
ATCTTGAGAATCGAATTTCA+TGG + chr2.3:1923879-1923898 MS.gene86338:CDS 30.0%
ATGAAGCTGAAACTGAATTT+GGG + chr2.3:1925707-1925726 MS.gene86338:CDS 30.0%
ATGATCCATTTCAGTAAAAC+AGG - chr2.3:1924919-1924938 None:intergenic 30.0%
ATTATTGGATCAAGATCAGA+TGG - chr2.3:1925140-1925159 None:intergenic 30.0%
CCTATAAAATGTTTCTTGTG+TGG + chr2.3:1924397-1924416 MS.gene86338:intron 30.0%
CTAATATAGATGTCAAAGCT+TGG + chr2.3:1924973-1924992 MS.gene86338:intron 30.0%
TGAAATTCGATTCTCAAGAT+CGG - chr2.3:1923879-1923898 None:intergenic 30.0%
! AATTTGAAGTTTCAAGGTCA+GGG - chr2.3:1923658-1923677 None:intergenic 30.0%
! CCACACAAGAAACATTTTAT+AGG - chr2.3:1924400-1924419 None:intergenic 30.0%
!! AAAGTCAATTTTGGTGAAGT+CGG - chr2.3:1924715-1924734 None:intergenic 30.0%
!! GATGATGGAACCTATTAAAT+CGG - chr2.3:1923612-1923631 None:intergenic 30.0%
!! TAACTTTGGAATATTGTTGC+TGG + chr2.3:1923510-1923529 MS.gene86338:CDS 30.0%
!! TTGTTATTAGGTTGGTTAAG+AGG + chr2.3:1923981-1924000 MS.gene86338:CDS 30.0%
!!! TTTTTGAGTTTGGATCATGA+TGG + chr2.3:1924004-1924023 MS.gene86338:CDS 30.0%
AACAAGCTCAACATCCAAAA+GGG - chr2.3:1923966-1923985 None:intergenic 35.0%
AATCAAGAGATATCGTTCGT+CGG + chr2.3:1924662-1924681 MS.gene86338:intron 35.0%
AATGTTTCTTGTGTGGTGAT+TGG + chr2.3:1924404-1924423 MS.gene86338:intron 35.0%
ACTCACATATATGCGTTTGT+AGG + chr2.3:1924616-1924635 MS.gene86338:intron 35.0%
ACTCTCTGATATACGTTTGT+TGG + chr2.3:1924440-1924459 MS.gene86338:intron 35.0%
AGATTTAGATTGCATGCTGT+TGG - chr2.3:1925189-1925208 None:intergenic 35.0%
ATTCCATTCAGTCAAGACAT+CGG - chr2.3:1925165-1925184 None:intergenic 35.0%
CCTTCTTAAAACAGCAAACA+TGG + chr2.3:1923829-1923848 MS.gene86338:CDS 35.0%
CGAAATGAACACACAACAAA+TGG + chr2.3:1925525-1925544 MS.gene86338:CDS 35.0%
CTTCTTTCAGTCATACTTGT+GGG + chr2.3:1923939-1923958 MS.gene86338:CDS 35.0%
GATGAAGCTGAAACTGAATT+TGG + chr2.3:1925706-1925725 MS.gene86338:CDS 35.0%
GCAATCAACATTTCTCTACA+TGG + chr2.3:1924846-1924865 MS.gene86338:intron 35.0%
GTCAAGACATCGGTAATTAT+TGG - chr2.3:1925155-1925174 None:intergenic 35.0%
TAACAAGCTCAACATCCAAA+AGG - chr2.3:1923967-1923986 None:intergenic 35.0%
TAACATACCATTAAGGTTGG+AGG + chr2.3:1925070-1925089 MS.gene86338:intron 35.0%
TAATGAAGCAATCCATGTCA+AGG - chr2.3:1923553-1923572 None:intergenic 35.0%
TCCAAACCAACAAGTAACTT+TGG + chr2.3:1923496-1923515 MS.gene86338:CDS 35.0%
TGCATGCATCAGTATATATG+TGG - chr2.3:1924884-1924903 None:intergenic 35.0%
TGTGTGACTTTCTAACTAGA+AGG - chr2.3:1924475-1924494 None:intergenic 35.0%
TTCCAAATCTAGATAGGACA+TGG + chr2.3:1924809-1924828 MS.gene86338:intron 35.0%
TTTGGTTAAGGTTGCTAAGT+TGG + chr2.3:1924045-1924064 MS.gene86338:CDS 35.0%
! AAACTGAATTTGGGTTTGGA+AGG + chr2.3:1925716-1925735 MS.gene86338:CDS 35.0%
! ATGTGCCTGTTTTACTGAAA+TGG + chr2.3:1924911-1924930 MS.gene86338:intron 35.0%
! GAATTTGAAGTTTCAAGGTC+AGG - chr2.3:1923659-1923678 None:intergenic 35.0%
! GATCATGATGGAGTTAAAAC+TGG + chr2.3:1924016-1924035 MS.gene86338:CDS 35.0%
! TCCAAAGTTACTTGTTGGTT+TGG - chr2.3:1923500-1923519 None:intergenic 35.0%
! TGATATACGTTTGTTGGACT+TGG + chr2.3:1924446-1924465 MS.gene86338:intron 35.0%
! TGTTGATTGCAACTAGCTAT+TGG - chr2.3:1924836-1924855 None:intergenic 35.0%
!! GATGGTCCATATTTTAAGCT+CGG - chr2.3:1925122-1925141 None:intergenic 35.0%
!! TATTAAAAGCATCGGAGAGA+TGG + chr2.3:1925669-1925688 MS.gene86338:CDS 35.0%
!! TGGATGTTGAGCTTGTTATT+AGG + chr2.3:1923969-1923988 MS.gene86338:CDS 35.0%
!! TGTTGAGCTTGTTATTAGGT+TGG + chr2.3:1923973-1923992 MS.gene86338:CDS 35.0%
!!! AGAGATTTTTTCCACTTAGG+AGG - chr2.3:1925790-1925809 None:intergenic 35.0%
!!! CCATGTTTGCTGTTTTAAGA+AGG - chr2.3:1923832-1923851 None:intergenic 35.0%
!!! GACAGAGATTTTTTCCACTT+AGG - chr2.3:1925793-1925812 None:intergenic 35.0%
!!! GGTTAAGAGGTTTTTGAGTT+TGG + chr2.3:1923994-1924013 MS.gene86338:CDS 35.0%
!!! GTACTAGTTTTCATGTCCAT+AGG - chr2.3:1925748-1925767 None:intergenic 35.0%
AACTGAAGGAAGACGTTTAC+AGG + chr2.3:1925557-1925576 MS.gene86338:CDS 40.0%
ACCGTTAGATTGACGAAGAT+CGG + chr2.3:1924509-1924528 MS.gene86338:intron 40.0%
ACGGTTGAGTTTCGTTTGTT+CGG - chr2.3:1925413-1925432 None:intergenic 40.0%
ACTTGAATAACAGCTCTCAC+AGG - chr2.3:1925382-1925401 None:intergenic 40.0%
ATCTCTTGATTGCGTGACTA+CGG - chr2.3:1924653-1924672 None:intergenic 40.0%
ATTGTTTCATGTGTGTTCGC+CGG + chr2.3:1924587-1924606 MS.gene86338:intron 40.0%
CAGCATCATGGATGAAGAAA+AGG + chr2.3:1923735-1923754 MS.gene86338:CDS 40.0%
CATATATGTGAGTCTCATGC+CGG - chr2.3:1924609-1924628 None:intergenic 40.0%
CATGATGCTGTTACACTTTC+AGG - chr2.3:1923725-1923744 None:intergenic 40.0%
CATGGAATGGAATCTTTGTC+TGG - chr2.3:1923794-1923813 None:intergenic 40.0%
CCACAAGTATGACTGAAAGA+AGG - chr2.3:1923941-1923960 None:intergenic 40.0%
CCTTCTTTCAGTCATACTTG+TGG + chr2.3:1923938-1923957 MS.gene86338:CDS 40.0%
CTAGTACTGTGGTCAAAAGT+AGG + chr2.3:1925761-1925780 MS.gene86338:CDS 40.0%
GCTCTACCGAGCTTAAAATA+TGG + chr2.3:1925113-1925132 MS.gene86338:intron 40.0%
GGACATGAAAACTAGTACTG+TGG + chr2.3:1925750-1925769 MS.gene86338:CDS 40.0%
GGTGTTCGTTCCGATTTAAT+AGG + chr2.3:1923599-1923618 MS.gene86338:CDS 40.0%
GTCCATGTCCTATCTAGATT+TGG - chr2.3:1924814-1924833 None:intergenic 40.0%
TAGTCCACTGAAATTGACAC+CGG - chr2.3:1924278-1924297 None:intergenic 40.0%
TATATCAGAGAGTCTCATGC+TGG - chr2.3:1924433-1924452 None:intergenic 40.0%
TCACTCACTATGCTTCCAAA+TGG + chr2.3:1923630-1923649 MS.gene86338:CDS 40.0%
TCCGATCTTCGTCAATCTAA+CGG - chr2.3:1924513-1924532 None:intergenic 40.0%
TCTCAGGTTCAAATTCTTCC+CGG + chr2.3:1924256-1924275 MS.gene86338:intron 40.0%
TTACCGATGTCTTGACTGAA+TGG + chr2.3:1925159-1925178 MS.gene86338:intron 40.0%
TTCATGCAGCTCAAAACGAA+CGG + chr2.3:1925353-1925372 MS.gene86338:CDS 40.0%
TTCGTCGGATTGACAAAGAT+CGG + chr2.3:1924677-1924696 MS.gene86338:intron 40.0%
! CTGAAAGTGTAACAGCATCA+TGG + chr2.3:1923723-1923742 MS.gene86338:CDS 40.0%
! GCTGAAACTGAATTTGGGTT+TGG + chr2.3:1925712-1925731 MS.gene86338:CDS 40.0%
!! AGTTAAAACTGGTGCTGCTT+TGG + chr2.3:1924027-1924046 MS.gene86338:CDS 40.0%
!! TGTGTAGGTTGATTGATAGC+AGG + chr2.3:1925308-1925327 MS.gene86338:CDS 40.0%
!! TGTTGGTTTGGACTTGTTTC+TGG - chr2.3:1923488-1923507 None:intergenic 40.0%
!!! TTGCTGTTTTAAGAAGGCGA+AGG - chr2.3:1923826-1923845 None:intergenic 40.0%
AAAGTAGGACTCCTCCTAAG+TGG + chr2.3:1925776-1925795 MS.gene86338:CDS 45.0%
AACTCAACCGTCACGTTGAT+TGG + chr2.3:1925422-1925441 MS.gene86338:CDS 45.0%
AAGCACTCCAATCAACGTGA+CGG - chr2.3:1925432-1925451 None:intergenic 45.0%
AAGGAGGAAGTTGCATGGAA+TGG - chr2.3:1923807-1923826 None:intergenic 45.0%
ACTAGCTCCTCCAACCTTAA+TGG - chr2.3:1925080-1925099 None:intergenic 45.0%
AGATGCATGCATCCTTGACA+TGG + chr2.3:1923538-1923557 MS.gene86338:CDS 45.0%
AGTCCACTGAAATTGACACC+GGG - chr2.3:1924277-1924296 None:intergenic 45.0%
CAATGTCATGCAAGCACAGA+TGG + chr2.3:1925594-1925613 MS.gene86338:CDS 45.0%
CATCATCAGTAGCTCTTGCA+TGG - chr2.3:1924153-1924172 None:intergenic 45.0%
CATGCAAGAGCTACTGATGA+TGG + chr2.3:1924151-1924170 MS.gene86338:CDS 45.0%
GATTGTTACCTTGCTGAAGC+TGG + chr2.3:1924070-1924089 MS.gene86338:CDS 45.0%
GGAATGGAATCTTTGTCTGG+TGG - chr2.3:1923791-1923810 None:intergenic 45.0%
GTGCTTCTTTCAGTAGCTTG+AGG + chr2.3:1925446-1925465 MS.gene86338:CDS 45.0%
GTTTGGAAGGCAAACACCTA+TGG + chr2.3:1925729-1925748 MS.gene86338:CDS 45.0%
TCAGTAGCTCTTGCATGGTT+AGG - chr2.3:1924148-1924167 None:intergenic 45.0%
TCTCTTGATTGCGTGACTAC+GGG - chr2.3:1924652-1924671 None:intergenic 45.0%
TTACCTTGCTGAAGCTGGTT+TGG + chr2.3:1924075-1924094 MS.gene86338:CDS 45.0%
TTGAGGAGTCCAAGTGGATA+CGG + chr2.3:1925463-1925482 MS.gene86338:CDS 45.0%
! GGAAGCATAGTGAGTGATGA+TGG - chr2.3:1923627-1923646 None:intergenic 45.0%
! TCATACTTGTGGGACCCTTT+TGG + chr2.3:1923949-1923968 MS.gene86338:CDS 45.0%
! TGGTGGAAGAACACTGACTA+AGG - chr2.3:1923774-1923793 None:intergenic 45.0%
!! AACTGGTGCTGCTTTGGTTA+AGG + chr2.3:1924033-1924052 MS.gene86338:CDS 45.0%
!! GTTTTGAGCTGCATGAAGTG+AGG - chr2.3:1925350-1925369 None:intergenic 45.0%
!! TCTCAGTTTGTTGCACTTGC+TGG + chr2.3:1924115-1924134 MS.gene86338:CDS 45.0%
!!! CTGTTTTAAGAAGGCGAAGG+AGG - chr2.3:1923823-1923842 None:intergenic 45.0%
AACACACCACCGTATCCACT+TGG - chr2.3:1925475-1925494 None:intergenic 50.0%
AAGTGGATACGGTGGTGTGT+TGG + chr2.3:1925474-1925493 MS.gene86338:CDS 50.0%
AGTAGCTTGAGGAGTCCAAG+TGG + chr2.3:1925457-1925476 MS.gene86338:CDS 50.0%
CATCCAAACCAGCTTCAGCA+AGG - chr2.3:1924081-1924100 None:intergenic 50.0%
CTTTCTTGCTTCGACACGCT+TGG - chr2.3:1925280-1925299 None:intergenic 50.0%
GCAAGCACAGATGGACAAGA+TGG + chr2.3:1925603-1925622 MS.gene86338:CDS 50.0%
TAGAAAGACACCGTGCCTGT+GGG - chr2.3:1925501-1925520 None:intergenic 50.0%
TTAGAAAGACACCGTGCCTG+TGG - chr2.3:1925502-1925521 None:intergenic 50.0%
TTCAAGGTCAGGGAGCCATT+TGG - chr2.3:1923648-1923667 None:intergenic 50.0%
! CTTCCCGGTGTCAATTTCAG+TGG + chr2.3:1924271-1924290 MS.gene86338:intron 50.0%
AGGAGTCCAAGTGGATACGG+TGG + chr2.3:1925466-1925485 MS.gene86338:CDS 55.0%
AGGCGAAGGAGGAAGTTGCA+TGG - chr2.3:1923812-1923831 None:intergenic 55.0%
GCTGCATGAAGTGAGGCTTC+TGG - chr2.3:1925343-1925362 None:intergenic 55.0%
GTGTGTTGGACCCACAGGCA+CGG + chr2.3:1925488-1925507 MS.gene86338:CDS 60.0%
CGGTGGTGTGTTGGACCCAC+AGG + chr2.3:1925483-1925502 MS.gene86338:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 1923461 1925816 1923461 ID=MS.gene86338
chr2.3 mRNA 1923461 1925816 1923461 ID=MS.gene86338.t1;Parent=MS.gene86338
chr2.3 exon 1923461 1924204 1923461 ID=MS.gene86338.t1.exon1;Parent=MS.gene86338.t1
chr2.3 CDS 1923461 1924204 1923461 ID=cds.MS.gene86338.t1;Parent=MS.gene86338.t1
chr2.3 exon 1925271 1925816 1925271 ID=MS.gene86338.t1.exon2;Parent=MS.gene86338.t1
chr2.3 CDS 1925271 1925816 1925271 ID=cds.MS.gene86338.t1;Parent=MS.gene86338.t1
Gene Sequence

>MS.gene86338

ATGAAGAAACAATGTTACAAAGAACCAGAAACAAGTCCAAACCAACAAGTAACTTTGGAATATTGTTGCTGGTTTGAAGATGCATGCATCCTTGACATGGATTGCTTCATTAAGACCCTTTCAAACATCAAACAAAAAGGTGTTCGTTCCGATTTAATAGGTTCCATCATCACTCACTATGCTTCCAAATGGCTCCCTGACCTTGAAACTTCAAATTCAAATTCAAATTCAAATTCAAATTCAAATGCAAATGCTAATTCACCTGAAAGTGTAACAGCATCATGGATGAAGAAAAGGTTTTTTGTTGAAACCTTAGTCAGTGTTCTTCCACCAGACAAAGATTCCATTCCATGCAACTTCCTCCTTCGCCTTCTTAAAACAGCAAACATGGTTCTTGTTGAGTCTACTTATCGAACCGATCTTGAGAATCGAATTTCATGGCAGTTAGATCAAGCTTCATTGAAAGAACTCATGATACCTTCTTTCAGTCATACTTGTGGGACCCTTTTGGATGTTGAGCTTGTTATTAGGTTGGTTAAGAGGTTTTTGAGTTTGGATCATGATGGAGTTAAAACTGGTGCTGCTTTGGTTAAGGTTGCTAAGTTGGTTGATTGTTACCTTGCTGAAGCTGGTTTGGATGCTAATTTGAGTTTGTCTCAGTTTGTTGCACTTGCTGGTGCTCTTCCTAACCATGCAAGAGCTACTGATGATGGTTTATATAGAGCCATTGACACTTATCTTAAAGTATGTTTTCTTTTTATCTTTTTCATTAGTTGAGTTAATTCTTTATAAAGATCTCAGGTTCAAATTCTTCCCGGTGTCAATTTCAGTGGACTAGTTTGACTTCTTAAGTGAATTTTTTTATACATACTCTTAATTTATTTTTTAATGACTAAGAAAGAAATAAGTATTAAGAGGAGGAGTAATTCTTGATATCCTATAAAATGTTTCTTGTGTGGTGATTGGTCACCAGCATGAGACTCTCTGATATACGTTTGTTGGACTTGGATTCCTTCTAGTTAGAAAGTCACACAATAAATAAATTTTGACCGTTAGATTGACGAAGATCGGACATTTCTTATTCTAACTTCAACAAATTGACTTCATAACATATCTCAAAATATGAATTGTTTCATGTGTGTTCGCCGGCATGAGACTCACATATATGCGTTTGTAGGACTCAAATTTCCCGTAGTCACGCAATCAAGAGATATCGTTCGTCGGATTGACAAAGATCGGACATTTTTTATTCCGACTTCACCAAAATTGACTTTACAAGTATATCTCAAAATACAAATTGTTTGTTTTTCATCTGAAGGTTGAGATTCACTACAGTGTGACCACAAAATTCCAAATCTAGATAGGACATGGACCAATAGCTAGTTGCAATCAACATTTCTCTACATGGCTTTTCTCTTGTCCACATATATACTGATGCATGCAAAATATGATGTGCCTGTTTTACTGAAATGGATCATAATTAATGATTGCTTGAGTATATGAAAACTCTATCTAATATAGATGTCAAAGCTTGGAACTTCCTTCCTTATAGATATGTATATTTATTACTTGTTTCCAAAAAGCACATTAATTTTCTTCTAAATTCAAGTAACATACCATTAAGGTTGGAGGAGCTAGTTGCAATCAACATTGCTCTACCGAGCTTAAAATATGGACCATCTGATCTTGATCCAATAATTACCGATGTCTTGACTGAATGGAATTCCAACAGCATGCAATCTAAATCTATTTATTCAGTCTGTATCTCATCTATAATGTGACACTGTCTATTTATTTATTTTTGCAATAGGCACATCCAAGCGTGTCGAAGCAAGAAAGAAAAAGTCTGTGTAGGTTGATTGATAGCAGGAAACTCACACCAGAAGCCTCACTTCATGCAGCTCAAAACGAACGGTTGCCTGTGAGAGCTGTTATTCAAGTTCTCTTCTCCGAACAAACGAAACTCAACCGTCACGTTGATTGGAGTGCTTCTTTCAGTAGCTTGAGGAGTCCAAGTGGATACGGTGGTGTGTTGGACCCACAGGCACGGTGTCTTTCTAAGCGCGAAATGAACACACAACAAATGGAGATAAAAAAACTGAAGGAAGACGTTTACAGGCTGCAAAGTCAGTGCAATGTCATGCAAGCACAGATGGACAAGATGGCTGAGAAGAAGAAAAGTTTCTTCAAATGGAAGAAACTTGCTTTTATTAAAAGCATCGGAGAGATGGAGAATGTTGAACAAGATGAAGCTGAAACTGAATTTGGGTTTGGAAGGCAAACACCTATGGACATGAAAACTAGTACTGTGGTCAAAAGTAGGACTCCTCCTAAGTGGAAAAAATCTCTGTCCTGA
Protein sequence

>MS.gene86338.t1

MKKQCYKEPETSPNQQVTLEYCCWFEDACILDMDCFIKTLSNIKQKGVRSDLIGSIITHYASKWLPDLETSNSNSNSNSNSNANANSPESVTASWMKKRFFVETLVSVLPPDKDSIPCNFLLRLLKTANMVLVESTYRTDLENRISWQLDQASLKELMIPSFSHTCGTLLDVELVIRLVKRFLSLDHDGVKTGAALVKVAKLVDCYLAEAGLDANLSLSQFVALAGALPNHARATDDGLYRAIDTYLKAHPSVSKQERKSLCRLIDSRKLTPEASLHAAQNERLPVRAVIQVLFSEQTKLNRHVDWSASFSSLRSPSGYGGVLDPQARCLSKREMNTQQMEIKKLKEDVYRLQSQCNVMQAQMDKMAEKKKSFFKWKKLAFIKSIGEMENVEQDEAETEFGFGRQTPMDMKTSTVVKSRTPPKWKKSLS