AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86464

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86464.t1 MTR_5g025500 88.889 135 14 1 1 135 1 134 6.13e-74 216
MS.gene86464.t1 MTR_5g005200 47.436 78 41 0 55 132 65 142 1.14e-23 89.7
MS.gene86464.t1 MTR_5g018010 43.529 85 46 2 52 135 183 266 9.01e-19 79.7
MS.gene86464.t1 MTR_5g018010 42.466 73 41 1 58 130 57 128 1.55e-17 76.6
MS.gene86464.t1 MTR_5g018010 43.529 85 46 2 52 135 260 343 1.28e-18 80.5
MS.gene86464.t1 MTR_5g018010 42.466 73 41 1 58 130 134 205 1.82e-17 77.0
MS.gene86464.t1 MTR_3g055330 38.384 99 60 1 34 132 138 235 3.31e-18 79.3
MS.gene86464.t1 MTR_3g055330 38.384 99 60 1 34 132 138 235 3.47e-18 78.6
MS.gene86464.t1 MTR_5g025520 37.662 77 48 0 55 131 26 102 2.89e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86464.t1 AT2G35605 55.303 132 35 3 4 135 2 109 9.64e-40 129
MS.gene86464.t1 AT1G31760 61.616 99 37 1 37 134 13 111 2.67e-36 120
MS.gene86464.t1 AT3G03590 59.259 81 33 0 55 135 63 143 1.45e-31 109
MS.gene86464.t1 AT2G14880 42.982 114 61 1 19 132 32 141 1.64e-29 104
MS.gene86464.t1 AT4G34290 47.674 86 45 0 47 132 59 144 1.90e-28 102
MS.gene86464.t1 AT3G48600 47.475 99 48 2 37 131 13 111 2.10e-23 90.1
MS.gene86464.t1 AT3G48600 47.475 99 48 2 37 131 13 111 8.05e-23 89.7
MS.gene86464.t1 AT4G26810 39.362 94 55 1 44 135 6 99 6.89e-19 76.3
MS.gene86464.t1 AT4G26810 39.362 94 55 1 44 135 6 99 6.89e-19 76.3
MS.gene86464.t1 AT4G22360 40.385 104 58 2 32 132 170 272 2.74e-18 79.7
MS.gene86464.t1 AT4G22360 36.585 82 49 2 56 135 305 385 8.83e-11 58.5
MS.gene86464.t1 AT3G19080 48.387 62 32 0 51 112 259 320 3.46e-15 71.2
MS.gene86464.t1 AT3G19080 38.462 78 47 1 54 131 114 190 2.85e-12 62.8
MS.gene86464.t1 AT1G49520 42.308 78 44 1 54 131 249 325 3.65e-15 70.9
MS.gene86464.t1 AT1G49520 38.462 78 47 1 54 131 100 176 2.80e-14 68.6
MS.gene86464.t1 AT3G19080 48.387 62 32 0 51 112 259 320 4.32e-15 70.9
MS.gene86464.t1 AT3G19080 40.260 77 43 2 60 134 387 462 1.68e-14 69.3
MS.gene86464.t1 AT3G19080 38.462 78 47 1 54 131 114 190 3.05e-12 62.8
MS.gene86464.t1 AT3G19080 48.387 62 32 0 51 112 259 320 4.32e-15 70.9
MS.gene86464.t1 AT3G19080 40.260 77 43 2 60 134 387 462 1.68e-14 69.3
MS.gene86464.t1 AT3G19080 38.462 78 47 1 54 131 114 190 3.05e-12 62.8
MS.gene86464.t1 AT3G19080 48.387 62 32 0 51 112 259 320 3.58e-14 68.2
MS.gene86464.t1 AT3G19080 40.260 77 43 2 60 134 385 460 1.15e-12 63.9
MS.gene86464.t1 AT3G19080 38.750 80 48 1 51 130 111 189 8.59e-12 61.6

Find 39 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATCTCCTTCTTGTTATT+AGG 0.242982 5.1:+15899342 None:intergenic
CTGAAGCTGTTAAGAAAGTT+TGG 0.251130 5.1:-15900550 MS.gene86464:CDS
TTTATCTTTCCCATCGAAAA+TGG 0.302387 5.1:+15899299 None:intergenic
TTGGTGCGCCTGAAGTTTCT+AGG 0.344233 5.1:-15900574 MS.gene86464:CDS
TTCAGCTTGATTTCACGAAA+TGG 0.371326 5.1:+15899241 None:intergenic
TATCAAGCTGCAGAATCTTC+AGG 0.384496 5.1:-15900522 MS.gene86464:intron
AAGAAGGCGGCTGCATCCTC+CGG 0.411297 5.1:-15900710 MS.gene86464:CDS
TCTGAGCTCGGTAATTTCAT+TGG 0.417141 5.1:-15900593 MS.gene86464:CDS
GAAATGGGTAGCTAGCAATT+TGG 0.423194 5.1:+15899257 None:intergenic
TGAAGCTGTTAAGAAAGTTT+GGG 0.446267 5.1:-15900549 MS.gene86464:CDS
CAGAATGCTAATGGCGGCGG+CGG 0.448004 5.1:-15900747 MS.gene86464:CDS
ATTGGCCGGCAGAATGCTAA+TGG 0.452798 5.1:-15900756 MS.gene86464:CDS
GACGGTTGTGGATGCAGCTC+CGG 0.455046 5.1:+15900691 None:intergenic
GCTTCAGTCCTAGAAACTTC+AGG 0.456584 5.1:+15900566 None:intergenic
AGGGATACAGAAGGTAGTTC+CGG 0.456709 5.1:-15900621 MS.gene86464:CDS
TCAGCTTGATTTCACGAAAT+GGG 0.467447 5.1:+15899242 None:intergenic
CTAATGGCGGCGGCGGCGAA+AGG 0.468371 5.1:-15900740 MS.gene86464:CDS
AGCTAGCAATTTGGCAATCT+CGG 0.474611 5.1:+15899266 None:intergenic
GGCGAAAGGAGCCACGAAGA+AGG 0.475511 5.1:-15900726 MS.gene86464:CDS
CGGCAGAATGCTAATGGCGG+CGG 0.486305 5.1:-15900750 MS.gene86464:CDS
GAAAACCGCCGTGAAGAAAA+CGG 0.496369 5.1:-15900663 MS.gene86464:CDS
GAAGAAAACGGTTACGAGCA+AGG 0.515768 5.1:-15900651 MS.gene86464:CDS
GGATGCAGCCGCCTTCTTCG+TGG 0.519626 5.1:+15900715 None:intergenic
TCGCGATCATGGCACCAGCT+AGG 0.520628 5.1:-15900790 None:intergenic
GCAGAATCCTAATAACAAGA+AGG 0.524697 5.1:-15899349 MS.gene86464:intron
CGCCGCCATTAGCATTCTGC+CGG 0.528951 5.1:+15900751 None:intergenic
GTTCCGGTCACTTCTGAGCT+CGG 0.533521 5.1:-15900605 MS.gene86464:CDS
ACGGTTACGAGCAAGGGTAC+CGG 0.543319 5.1:-15900644 MS.gene86464:CDS
AATGCTCCGAAAACCCTAGC+TGG 0.551067 5.1:+15900776 None:intergenic
TTTCGATGGGAAAGATAAAG+TGG 0.555020 5.1:-15899295 MS.gene86464:CDS
AACTACCTTCTGTATCCCTC+CGG 0.556811 5.1:+15900625 None:intergenic
AAGAAAACGGTTACGAGCAA+GGG 0.571769 5.1:-15900650 MS.gene86464:CDS
GAAAGGAGCCACGAAGAAGG+CGG 0.599028 5.1:-15900723 MS.gene86464:CDS
GGTTGTGGATGCAGCTCCGG+AGG 0.611120 5.1:+15900694 None:intergenic
TTACCGAGCTCAGAAGTGAC+CGG 0.624475 5.1:+15900602 None:intergenic
GGCCGGCAGAATGCTAATGG+CGG 0.629027 5.1:-15900753 MS.gene86464:CDS
GTTACGAGCAAGGGTACCGG+AGG 0.664192 5.1:-15900641 MS.gene86464:CDS
GGGTACCGGAGGGATACAGA+AGG 0.705233 5.1:-15900630 MS.gene86464:CDS
TTACGAGCAAGGGTACCGGA+GGG 0.744071 5.1:-15900640 MS.gene86464:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAGGATATTATTTAT+AGG + chr5.1:15900346-15900365 None:intergenic 10.0%
!! TAAAGTTTATTTAAAAGAAA+AGG - chr5.1:15900091-15900110 MS.gene86464:intron 10.0%
!!! ATATAATAATAGTTTGAAAT+CGG - chr5.1:15900155-15900174 MS.gene86464:intron 10.0%
!!! TCATTTTCTTAAATTAATAT+GGG - chr5.1:15900014-15900033 MS.gene86464:intron 10.0%
!!! TTTTTGAAATTAAAAATGAA+TGG - chr5.1:15899801-15899820 MS.gene86464:intron 10.0%
!!! GTCATTTTCTTAAATTAATA+TGG - chr5.1:15900013-15900032 MS.gene86464:intron 15.0%
!! AAACAAACATCTCAAAAATA+AGG + chr5.1:15900360-15900379 None:intergenic 20.0%
!! ATAACTAATGTAATCATTGT+TGG - chr5.1:15900404-15900423 MS.gene86464:intron 20.0%
!! TTTGTTCTGTTATTAAATTG+TGG - chr5.1:15900503-15900522 MS.gene86464:intron 20.0%
!!! AAAACTTTGATTAGGTTTTA+TGG - chr5.1:15899623-15899642 MS.gene86464:intron 20.0%
!!! ATTGTATTTTTGTCTCTTTT+TGG - chr5.1:15900604-15900623 MS.gene86464:CDS 20.0%
! AAGAGACAAAAATACAATAC+AGG + chr5.1:15900602-15900621 None:intergenic 25.0%
! ATTTAAGAAAGAGAAAAGGA+TGG + chr5.1:15900477-15900496 None:intergenic 25.0%
! CTGAATGAAAAACTTTGATT+AGG - chr5.1:15899615-15899634 MS.gene86464:intron 25.0%
! GACAATTTAAGAAAGAGAAA+AGG + chr5.1:15900481-15900500 None:intergenic 25.0%
!!! CAATTTTGATTAGTAGTAGA+TGG - chr5.1:15900289-15900308 MS.gene86464:intron 25.0%
!!! TTTTGAGATGTTTGTTTCTT+TGG - chr5.1:15900363-15900382 MS.gene86464:intron 25.0%
AGACAAAAATACAATACAGG+AGG + chr5.1:15900599-15900618 None:intergenic 30.0%
CTGAATGTAATCAAATGTGT+TGG + chr5.1:15899989-15900008 None:intergenic 30.0%
CTGTTTACATAATGGCTTAA+TGG - chr5.1:15900632-15900651 MS.gene86464:CDS 30.0%
TGAAGCTGTTAAGAAAGTTT+GGG - chr5.1:15899475-15899494 MS.gene86464:intron 30.0%
TTTATCTTTCCCATCGAAAA+TGG + chr5.1:15900728-15900747 None:intergenic 30.0%
TTTCAAAAATCACACGAAAC+AGG + chr5.1:15899790-15899809 None:intergenic 30.0%
! AGTTTCTGTTGTCTGATTTT+TGG - chr5.1:15900198-15900217 MS.gene86464:intron 30.0%
! TGATTTTGCGCTATTTGATT+TGG - chr5.1:15899648-15899667 MS.gene86464:intron 30.0%
! TGGTTATGATTTTCTTAAGC+AGG - chr5.1:15900309-15900328 MS.gene86464:intron 30.0%
! TTTTATTCTGCTGTTCATAC+TGG - chr5.1:15900428-15900447 MS.gene86464:intron 30.0%
!! AAGATCTCCTTCTTGTTATT+AGG + chr5.1:15900685-15900704 None:intergenic 30.0%
!! ATTGTTTGTTGTAATCGCAA+TGG - chr5.1:15899673-15899692 MS.gene86464:intron 30.0%
!! ATTTTTGTCTCTTTTTGGTG+TGG - chr5.1:15900609-15900628 MS.gene86464:CDS 30.0%
CTGAAGCTGTTAAGAAAGTT+TGG - chr5.1:15899474-15899493 MS.gene86464:intron 35.0%
GCAGAATCCTAATAACAAGA+AGG - chr5.1:15900675-15900694 MS.gene86464:CDS 35.0%
TCAGCTTGATTTCACGAAAT+GGG + chr5.1:15900785-15900804 None:intergenic 35.0%
TCTGATGTTTGTGTTTGTGT+TGG - chr5.1:15900050-15900069 MS.gene86464:intron 35.0%
TTTCGATGGGAAAGATAAAG+TGG - chr5.1:15900729-15900748 MS.gene86464:CDS 35.0%
! AAACTGAAGACCATTTTCGA+TGG - chr5.1:15900715-15900734 MS.gene86464:CDS 35.0%
! AACTGAAGACCATTTTCGAT+GGG - chr5.1:15900716-15900735 MS.gene86464:CDS 35.0%
! AATCGCAATGGTGTGTTTTA+GGG - chr5.1:15899685-15899704 MS.gene86464:intron 35.0%
! TAATCGCAATGGTGTGTTTT+AGG - chr5.1:15899684-15899703 MS.gene86464:intron 35.0%
! TTATTGTTGTTGCGTTCATG+AGG - chr5.1:15899738-15899757 MS.gene86464:intron 35.0%
!! AGAAGTGTCAAGTGTTATTG+AGG + chr5.1:15899917-15899936 None:intergenic 35.0%
!! TTCTGTTGTCTGATTTTTGG+AGG - chr5.1:15900201-15900220 MS.gene86464:intron 35.0%
AAGAAAACGGTTACGAGCAA+GGG - chr5.1:15899374-15899393 MS.gene86464:intron 40.0%
AGCTAGCAATTTGGCAATCT+CGG + chr5.1:15900761-15900780 None:intergenic 40.0%
ATCCAAGTCAAACTATCCTG+CGG + chr5.1:15900564-15900583 None:intergenic 40.0%
CACTTGACACTTCTGATTGA+AGG - chr5.1:15899923-15899942 MS.gene86464:intron 40.0%
GAAATGGGTAGCTAGCAATT+TGG + chr5.1:15900770-15900789 None:intergenic 40.0%
TATCAAGCTGCAGAATCTTC+AGG - chr5.1:15899502-15899521 MS.gene86464:intron 40.0%
TCTGAGCTCGGTAATTTCAT+TGG - chr5.1:15899431-15899450 MS.gene86464:intron 40.0%
TGGTGTGGCTGTTTACATAA+TGG - chr5.1:15900624-15900643 MS.gene86464:CDS 40.0%
!! CAAATGTGTTGGTGTCATGT+CGG + chr5.1:15899978-15899997 None:intergenic 40.0%
!! CAAGTTTGAGTTGTGCTATC+TGG - chr5.1:15900227-15900246 MS.gene86464:intron 40.0%
AAACTCTAGCCATCGCAACA+TGG + chr5.1:15900183-15900202 None:intergenic 45.0%
AACTACCTTCTGTATCCCTC+CGG + chr5.1:15899402-15899421 None:intergenic 45.0%
AGGGATACAGAAGGTAGTTC+CGG - chr5.1:15899403-15899422 MS.gene86464:intron 45.0%
GAAAACCGCCGTGAAGAAAA+CGG - chr5.1:15899361-15899380 MS.gene86464:intron 45.0%
GAAGAAAACGGTTACGAGCA+AGG - chr5.1:15899373-15899392 MS.gene86464:intron 45.0%
GCTTCAGTCCTAGAAACTTC+AGG + chr5.1:15899461-15899480 None:intergenic 45.0%
GTATATTGCTTAACAGCCGC+AGG - chr5.1:15900545-15900564 MS.gene86464:CDS 45.0%
TAACCATCTCACCATCGCAA+GGG + chr5.1:15900269-15900288 None:intergenic 45.0%
TTTCTTTGCGATGTTGCGTC+TGG - chr5.1:15899855-15899874 MS.gene86464:intron 45.0%
! GCTCGTAACCGTTTTCTTCA+CGG + chr5.1:15899372-15899391 None:intergenic 45.0%
!! TGTTTGTGTTGGTGCTGCAT+AGG - chr5.1:15900061-15900080 MS.gene86464:intron 45.0%
AAATCGGAGCCATGTTGCGA+TGG - chr5.1:15900171-15900190 MS.gene86464:intron 50.0%
AATGCTCCGAAAACCCTAGC+TGG + chr5.1:15899251-15899270 None:intergenic 50.0%
ACCATCGCAAGGGCTTCAAA+AGG + chr5.1:15900259-15900278 None:intergenic 50.0%
AGCCGCAGGATAGTTTGACT+TGG - chr5.1:15900559-15900578 MS.gene86464:CDS 50.0%
ATTGGCCGGCAGAATGCTAA+TGG - chr5.1:15899268-15899287 MS.gene86464:CDS 50.0%
CCATCGCAAGGGCTTCAAAA+GGG + chr5.1:15900258-15900277 None:intergenic 50.0%
GTAACCATCTCACCATCGCA+AGG + chr5.1:15900270-15900289 None:intergenic 50.0%
TTACCGAGCTCAGAAGTGAC+CGG + chr5.1:15899425-15899444 None:intergenic 50.0%
TTGGTGCGCCTGAAGTTTCT+AGG - chr5.1:15899450-15899469 MS.gene86464:intron 50.0%
! AGCTAGGGTTTTCGGAGCAT+TGG - chr5.1:15899250-15899269 MS.gene86464:CDS 50.0%
! ATGGCACCAGCTAGGGTTTT+CGG - chr5.1:15899242-15899261 MS.gene86464:CDS 50.0%
! CGTAACCGTTTTCTTCACGG+CGG + chr5.1:15899369-15899388 None:intergenic 50.0%
! GGTTTTCTTCACGACGGTTG+TGG + chr5.1:15899348-15899367 None:intergenic 50.0%
! TTGAAGGTGTGTCCATCGTC+CGG - chr5.1:15899939-15899958 MS.gene86464:intron 50.0%
!! TGTCGGTGTCAAACACATGC+CGG + chr5.1:15899961-15899980 None:intergenic 50.0%
AAGCCCTTGCGATGGTGAGA+TGG - chr5.1:15900263-15900282 MS.gene86464:intron 55.0%
GAAAGGAGCCACGAAGAAGG+CGG - chr5.1:15899301-15899320 MS.gene86464:CDS 55.0%
GTCAAACACATGCCGGACGA+TGG + chr5.1:15899954-15899973 None:intergenic 55.0%
GTTCCGGTCACTTCTGAGCT+CGG - chr5.1:15899419-15899438 MS.gene86464:intron 55.0%
! ACGGTTACGAGCAAGGGTAC+CGG - chr5.1:15899380-15899399 MS.gene86464:intron 55.0%
! CACGGCGGTTTTCTTCACGA+CGG + chr5.1:15899354-15899373 None:intergenic 55.0%
!! TTACGAGCAAGGGTACCGGA+GGG - chr5.1:15899384-15899403 MS.gene86464:intron 55.0%
!!! AGGGTTTTCGGAGCATTGGC+CGG - chr5.1:15899254-15899273 MS.gene86464:CDS 55.0%
!!! CCCTTTTGAAGCCCTTGCGA+TGG - chr5.1:15900255-15900274 MS.gene86464:intron 55.0%
AAGAAGGCGGCTGCATCCTC+CGG - chr5.1:15899314-15899333 MS.gene86464:CDS 60.0%
CAGAATGCTAATGGCGGCGG+CGG - chr5.1:15899277-15899296 MS.gene86464:CDS 60.0%
CGCCGCCATTAGCATTCTGC+CGG + chr5.1:15899276-15899295 None:intergenic 60.0%
CGGCAGAATGCTAATGGCGG+CGG - chr5.1:15899274-15899293 MS.gene86464:CDS 60.0%
GACGGTTGTGGATGCAGCTC+CGG + chr5.1:15899336-15899355 None:intergenic 60.0%
GGCCGGCAGAATGCTAATGG+CGG - chr5.1:15899271-15899290 MS.gene86464:CDS 60.0%
GGCGAAAGGAGCCACGAAGA+AGG - chr5.1:15899298-15899317 MS.gene86464:CDS 60.0%
!! GGGTACCGGAGGGATACAGA+AGG - chr5.1:15899394-15899413 MS.gene86464:intron 60.0%
!! GTTACGAGCAAGGGTACCGG+AGG - chr5.1:15899383-15899402 MS.gene86464:intron 60.0%
GGATGCAGCCGCCTTCTTCG+TGG + chr5.1:15899312-15899331 None:intergenic 65.0%
GGTTGTGGATGCAGCTCCGG+AGG + chr5.1:15899333-15899352 None:intergenic 65.0%
CTAATGGCGGCGGCGGCGAA+AGG - chr5.1:15899284-15899303 MS.gene86464:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 15899242 15900804 15899242 ID=MS.gene86464
chr5.1 mRNA 15899242 15900804 15899242 ID=MS.gene86464.t1;Parent=MS.gene86464
chr5.1 exon 15900523 15900804 15900523 ID=MS.gene86464.t1.exon1;Parent=MS.gene86464.t1
chr5.1 CDS 15900523 15900804 15900523 ID=cds.MS.gene86464.t1;Parent=MS.gene86464.t1
chr5.1 exon 15899242 15899367 15899242 ID=MS.gene86464.t1.exon2;Parent=MS.gene86464.t1
chr5.1 CDS 15899242 15899367 15899242 ID=cds.MS.gene86464.t1;Parent=MS.gene86464.t1
Gene Sequence

>MS.gene86464

ATGGCACCAGCTAGGGTTTTCGGAGCATTGGCCGGCAGAATGCTAATGGCGGCGGCGGCGAAAGGAGCCACGAAGAAGGCGGCTGCATCCTCCGGAGCTGCATCCACAACCGTCGTGAAGAAAACCGCCGTGAAGAAAACGGTTACGAGCAAGGGTACCGGAGGGATACAGAAGGTAGTTCCGGTCACTTCTGAGCTCGGTAATTTCATTGGTGCGCCTGAAGTTTCTAGGACTGAAGCTGTTAAGAAAGTTTGGGAGTATATCAAGCTGCAGAATCTTCAGGTTTTGTTTTCGTTTTCACTCAATGCTTCTATTTTCACTCTGCGATCTTAGATTTTATTTTTTCATTTCTCGTTTGAAATTAATCTTGATACTGAATGAAAAACTTTGATTAGGTTTTATGGATTGATTTTGCGCTATTTGATTTGGTTATTGTTTGTTGTAATCGCAATGGTGTGTTTTAGGGTTAACTTTTTGAATGTTGTGATTGATATTGTTATTGTTGTTGCGTTCATGAGGCTTGTCATTATCTATTTGTGATGAAACCTGTTTCGTGTGATTTTTGAAATTAAAAATGAATGGTAATTTGTATTGAGCTTATCTGTTATATCTGTTTCTTTGCGATGTTGCGTCTGGAAAGTATAGAATCATTCAGAAACAATGTAGTCGTTGCCTCAATAACACTTGACACTTCTGATTGAAGGTGTGTCCATCGTCCGGCATGTGTTTGACACCGACATGACACCAACACATTTGATTACATTCAGTTGTGTCATTTTCTTAAATTAATATGGGTATCGAGTCAGTGTCTGATGTTTGTGTTTGTGTTGGTGCTGCATAGGTCACTGCTAAAGTTTATTTAAAAGAAAAGGTATTGTTGCATTGCAGTAAGATATTTAGTATGCATTGTGCAATATAATAATAGTTTGAAATCGGAGCCATGTTGCGATGGCTAGAGTTTCTGTTGTCTGATTTTTGGAGGTCACAAGTTTGAGTTGTGCTATCTGGTCTAACCCTTTTGAAGCCCTTGCGATGGTGAGATGGTTACAATTTTGATTAGTAGTAGATGGTTATGATTTTCTTAAGCAGGTTTGTCGTGTTCCTATAAATAATATCCTTATTTTTGAGATGTTTGTTTCTTTGGTTCATTTGTTAGCTGCTGATAACTAATGTAATCATTGTTGGATTTTATTCTGCTGTTCATACTGGCATTTCAGTACTCTGTTTTGTTTCCATCCTTTTCTCTTTCTTAAATTGTCTGTTTGTTCTGTTATTAAATTGTGGAAGCTTTCAAAGTTTCATTGTATATTGCTTAACAGCCGCAGGATAGTTTGACTTGGATTATCAAACTTATCCTCCTGTATTGTATTTTTGTCTCTTTTTGGTGTGGCTGTTTACATAATGGCTTAATGGCTTAACAACGATATTGTTTTGCAGAATCCTAATAACAAGAAGGAGATCTTTTGTGATGACAAACTGAAGACCATTTTCGATGGGAAAGATAAAGTGGTCTTCACCGAGATTGCCAAATTGCTAGCTACCCATTTCGTGAAATCAAGCTGA
Protein sequence

>MS.gene86464.t1

MAPARVFGALAGRMLMAAAAKGATKKAAASSGAASTTVVKKTAVKKTVTSKGTGGIQKVVPVTSELGNFIGAPEVSRTEAVKKVWEYIKLQNLQNPNNKKEIFCDDKLKTIFDGKDKVVFTEIAKLLATHFVKSS