Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73121.t1 | XP_024633585.1 | 98.9 | 95 | 1 | 0 | 1 | 95 | 6 | 100 | 3.80E-46 | 193.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73121.t1 | Q08747 | 39.0 | 77 | 44 | 3 | 19 | 95 | 124 | 197 | 7.8e-08 | 57.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73121.t1 | I3SKK8 | 98.9 | 95 | 1 | 0 | 1 | 95 | 6 | 100 | 2.7e-46 | 193.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene73121.t1 | TR | SWI/SNF-BAF60b |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene82460 | MS.gene73121 | PPI |
| MS.gene86464 | MS.gene73121 | PPI |
| MS.gene83494 | MS.gene73121 | PPI |
| MS.gene72734 | MS.gene73121 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73121.t1 | MTR_3g054150 | 86.885 | 61 | 8 | 0 | 1 | 61 | 6 | 66 | 3.00e-32 | 110 |
| MS.gene73121.t1 | MTR_3g054160 | 97.674 | 43 | 1 | 0 | 53 | 95 | 34 | 76 | 1.14e-24 | 88.6 |
| MS.gene73121.t1 | MTR_5g025500 | 41.304 | 92 | 52 | 1 | 1 | 92 | 43 | 132 | 3.23e-18 | 73.9 |
| MS.gene73121.t1 | MTR_5g005200 | 42.424 | 66 | 38 | 0 | 25 | 90 | 76 | 141 | 3.90e-17 | 71.2 |
| MS.gene73121.t1 | MTR_5g018010 | 47.170 | 53 | 28 | 0 | 19 | 71 | 269 | 321 | 6.58e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73121.t1 | AT4G26810 | 82.105 | 95 | 17 | 0 | 1 | 95 | 6 | 100 | 1.35e-53 | 162 |
| MS.gene73121.t1 | AT4G26810 | 82.105 | 95 | 17 | 0 | 1 | 95 | 6 | 100 | 1.35e-53 | 162 |
| MS.gene73121.t1 | AT2G35605 | 37.500 | 96 | 56 | 1 | 1 | 92 | 12 | 107 | 2.75e-18 | 73.6 |
| MS.gene73121.t1 | AT1G31760 | 44.118 | 68 | 38 | 0 | 25 | 92 | 43 | 110 | 1.26e-16 | 69.3 |
| MS.gene73121.t1 | AT4G34290 | 45.455 | 66 | 36 | 0 | 25 | 90 | 78 | 143 | 5.81e-15 | 65.9 |
| MS.gene73121.t1 | AT2G14880 | 42.424 | 66 | 38 | 0 | 25 | 90 | 75 | 140 | 4.16e-14 | 63.5 |
| MS.gene73121.t1 | AT3G03590 | 41.176 | 68 | 40 | 0 | 25 | 92 | 74 | 141 | 1.23e-13 | 62.4 |
Find 15 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACATCACTTGGGCTCTTT+CGG | 0.308494 | 3.4:-50828771 | None:intergenic |
| ACGAAGTCTCTGAGGGTAGA+AGG | 0.418915 | 3.4:-50826544 | None:intergenic |
| AAAAGGAACCTGGAGATTGT+TGG | 0.457574 | 3.4:-50826626 | None:intergenic |
| GGACCAAACACGCATAAAAC+AGG | 0.459688 | 3.4:-50826593 | None:intergenic |
| AAATGCAGCTTGATAAGTGC+TGG | 0.495546 | 3.4:-50828747 | None:intergenic |
| TTTGTATCACACACATCACT+TGG | 0.501644 | 3.4:-50828781 | None:intergenic |
| GTTAACGAGATCAATTAAGT+TGG | 0.512557 | 3.4:-50826518 | None:intergenic |
| AACTGTGATGAGAAGTTGAA+AGG | 0.554499 | 3.4:+50828684 | MS.gene73121:CDS |
| CATCAAAACCAACAATCTCC+AGG | 0.575838 | 3.4:+50826618 | MS.gene73121:CDS |
| TTGTATCACACACATCACTT+GGG | 0.615328 | 3.4:-50828780 | None:intergenic |
| ATTGACCGACGAAGTCTCTG+AGG | 0.633096 | 3.4:-50826552 | None:intergenic |
| AGTCTCTGAGGGTAGAAGGA+AGG | 0.634889 | 3.4:-50826540 | None:intergenic |
| AGTTCTGCTAAATCAACCTG+AGG | 0.655726 | 3.4:-50828723 | None:intergenic |
| TTGACCGACGAAGTCTCTGA+GGG | 0.668063 | 3.4:-50826551 | None:intergenic |
| TCTACCCTCAGAGACTTCGT+CGG | 0.681938 | 3.4:+50826547 | MS.gene73121:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTTATTATAATTAAAT+AGG | + | chr3.4:50826876-50826895 | MS.gene73121:intron | 0.0% |
| !! | AAAAAAGATTTAAATATTGG+GGG | - | chr3.4:50827671-50827690 | None:intergenic | 15.0% |
| !! | CAAAAAAGATTTAAATATTG+GGG | - | chr3.4:50827672-50827691 | None:intergenic | 15.0% |
| !! | CCAAAAAAGATTTAAATATT+GGG | - | chr3.4:50827673-50827692 | None:intergenic | 15.0% |
| !! | TCCAAAAAAGATTTAAATAT+TGG | - | chr3.4:50827674-50827693 | None:intergenic | 15.0% |
| !! | TGACACAAAATAAAAAATAA+AGG | - | chr3.4:50828132-50828151 | None:intergenic | 15.0% |
| !!! | AATAAATTTGATAAAAGCAT+AGG | - | chr3.4:50826703-50826722 | None:intergenic | 15.0% |
| !!! | TTTACCATATTTTTGTTATT+TGG | - | chr3.4:50828666-50828685 | None:intergenic | 15.0% |
| !!! | TTTGATTTGAAAAGAAAAAA+AGG | - | chr3.4:50826646-50826665 | None:intergenic | 15.0% |
| !! | AAAAAGATTTAAATATTGGG+GGG | - | chr3.4:50827670-50827689 | None:intergenic | 20.0% |
| !! | AAATTAAGTTAAAAGATCAC+TGG | + | chr3.4:50828562-50828581 | MS.gene73121:intron | 20.0% |
| !! | ATAACACTATTAAAACTTCT+AGG | - | chr3.4:50827421-50827440 | None:intergenic | 20.0% |
| !! | ATAAGACATCATAACAAATA+TGG | - | chr3.4:50828059-50828078 | None:intergenic | 20.0% |
| !!! | ATATTTGTTATGATGTCTTA+TGG | + | chr3.4:50828058-50828077 | MS.gene73121:intron | 20.0% |
| !!! | CCCAATATTTAAATCTTTTT+TGG | + | chr3.4:50827670-50827689 | MS.gene73121:intron | 20.0% |
| ! | AATTAAATAGGTAGAGTAGA+GGG | + | chr3.4:50826888-50826907 | MS.gene73121:intron | 25.0% |
| ! | ACATCTTCAATCTAAATTGT+TGG | - | chr3.4:50827100-50827119 | None:intergenic | 25.0% |
| ! | ACTAAGAAATAAAGTTGTTC+AGG | - | chr3.4:50828186-50828205 | None:intergenic | 25.0% |
| ! | GGATCCAAATAACAAAAATA+TGG | + | chr3.4:50828659-50828678 | MS.gene73121:intron | 25.0% |
| ! | TAACAATATATCAGCATTGT+CGG | - | chr3.4:50826766-50826785 | None:intergenic | 25.0% |
| ! | TAACATGCATAGATGAAAAA+TGG | + | chr3.4:50828455-50828474 | MS.gene73121:intron | 25.0% |
| ! | TAATGTAACATTGATACATG+TGG | + | chr3.4:50827135-50827154 | MS.gene73121:intron | 25.0% |
| ! | TAATTAAATAGGTAGAGTAG+AGG | + | chr3.4:50826887-50826906 | MS.gene73121:intron | 25.0% |
| ! | TTTAATAGTGTTATCAATCC+CGG | + | chr3.4:50827427-50827446 | MS.gene73121:intron | 25.0% |
| !! | AGTTAGAAATAATGGAAGAT+CGG | + | chr3.4:50827034-50827053 | MS.gene73121:intron | 25.0% |
| !!! | ATATTATGTTGATAGTGCTT+TGG | - | chr3.4:50827911-50827930 | None:intergenic | 25.0% |
| !!! | ATTAAGTTGGCAAGTTTTTT+AGG | - | chr3.4:50826508-50826527 | None:intergenic | 25.0% |
| !!! | GAAAAGGATCTGTTTTATTT+TGG | - | chr3.4:50827935-50827954 | None:intergenic | 25.0% |
| !!! | TTAAGTTGGCAAGTTTTTTA+GGG | - | chr3.4:50826507-50826526 | None:intergenic | 25.0% |
| !!! | TTTGATTTGATCTGCTAAAA+AGG | + | chr3.4:50827216-50827235 | MS.gene73121:intron | 25.0% |
| !!! | TTTTAGTTTTTTCACTTGTG+TGG | + | chr3.4:50826984-50827003 | MS.gene73121:intron | 25.0% |
| AAGGTTGGAGTTAGAAATAA+TGG | + | chr3.4:50827026-50827045 | MS.gene73121:intron | 30.0% | |
| AGAACTAACACAATAGACAT+AGG | - | chr3.4:50827326-50827345 | None:intergenic | 30.0% | |
| ATCAACTATAAGCAAAAGTG+CGG | - | chr3.4:50827366-50827385 | None:intergenic | 30.0% | |
| CAGTCAATAGCATAATATAG+TGG | - | chr3.4:50827807-50827826 | None:intergenic | 30.0% | |
| CATAGATGAAAAATGGAGTT+TGG | + | chr3.4:50828462-50828481 | MS.gene73121:intron | 30.0% | |
| GAAACTTTAAGCCAAGTTAA+AGG | - | chr3.4:50828101-50828120 | None:intergenic | 30.0% | |
| GAACTAACACAATAGACATA+GGG | - | chr3.4:50827325-50827344 | None:intergenic | 30.0% | |
| GACAATGCTGATATATTGTT+AGG | + | chr3.4:50826765-50826784 | MS.gene73121:intron | 30.0% | |
| GTTAACGAGATCAATTAAGT+TGG | - | chr3.4:50826521-50826540 | None:intergenic | 30.0% | |
| TATGTCTATTGTGTTAGTTC+TGG | + | chr3.4:50827325-50827344 | MS.gene73121:intron | 30.0% | |
| TCAATAGCATAATATAGTGG+TGG | - | chr3.4:50827804-50827823 | None:intergenic | 30.0% | |
| TGAAAAGAAAAAAAGGAACC+TGG | - | chr3.4:50826639-50826658 | None:intergenic | 30.0% | |
| ! | AAATAATGGAAGATCGGAAT+AGG | + | chr3.4:50827040-50827059 | MS.gene73121:intron | 30.0% |
| ! | AATAATGGAAGATCGGAATA+GGG | + | chr3.4:50827041-50827060 | MS.gene73121:intron | 30.0% |
| ! | AATTGTTTCTTTGATAGAGC+AGG | + | chr3.4:50828638-50828657 | MS.gene73121:intron | 30.0% |
| ! | GATGTAATTCACCTTTTGAA+TGG | + | chr3.4:50828415-50828434 | MS.gene73121:intron | 30.0% |
| ! | GTTATGATGTCTTATGGTTT+TGG | + | chr3.4:50828064-50828083 | MS.gene73121:intron | 30.0% |
| ! | TTAAGCTAGAGAGTTTTCAA+GGG | + | chr3.4:50826929-50826948 | MS.gene73121:intron | 30.0% |
| !! | GAACTTTTTGTGTTATTCTG+AGG | + | chr3.4:50827692-50827711 | MS.gene73121:intron | 30.0% |
| !!! | AAGTTGAAAGGCATTTTACT+TGG | + | chr3.4:50828696-50828715 | MS.gene73121:CDS | 30.0% |
| !!! | AGTTGAAAGGCATTTTACTT+GGG | + | chr3.4:50828697-50828716 | MS.gene73121:CDS | 30.0% |
| !!! | TTATGGTTTTGGTATACTGT+GGG | + | chr3.4:50828075-50828094 | MS.gene73121:intron | 30.0% |
| AACTGTGATGAGAAGTTGAA+AGG | + | chr3.4:50828684-50828703 | MS.gene73121:CDS | 35.0% | |
| ATGCTGATATATTGTTAGGC+TGG | + | chr3.4:50826769-50826788 | MS.gene73121:intron | 35.0% | |
| CAGCTCAATAACATCAGAAA+AGG | - | chr3.4:50827951-50827970 | None:intergenic | 35.0% | |
| GCTGACAGGTTTAAACATAA+AGG | + | chr3.4:50828494-50828513 | MS.gene73121:intron | 35.0% | |
| GTCTTACTTGACCATTCAAA+AGG | - | chr3.4:50828429-50828448 | None:intergenic | 35.0% | |
| TAGAGTAGAGGGTAAGATAT+GGG | + | chr3.4:50826899-50826918 | MS.gene73121:intron | 35.0% | |
| TAGTTGTAGATCAGTGAGAA+GGG | + | chr3.4:50827559-50827578 | MS.gene73121:intron | 35.0% | |
| TTTAAACCTGTCAGCAATGA+AGG | - | chr3.4:50828489-50828508 | None:intergenic | 35.0% | |
| TTTAAATATTGGGGGGTCTA+AGG | - | chr3.4:50827663-50827682 | None:intergenic | 35.0% | |
| ! | CTTAAGCTAGAGAGTTTTCA+AGG | + | chr3.4:50826928-50826947 | MS.gene73121:intron | 35.0% |
| ! | GAATGGTCAAGTAAGACTTT+TGG | + | chr3.4:50828432-50828451 | MS.gene73121:intron | 35.0% |
| ! | TAGTGAATTGTGATAACGAG+AGG | + | chr3.4:50827475-50827494 | MS.gene73121:intron | 35.0% |
| ! | TGAGTGGTAGTGATGAAATA+AGG | + | chr3.4:50827007-50827026 | MS.gene73121:intron | 35.0% |
| ! | TGGTAGTGATGAAATAAGGT+TGG | + | chr3.4:50827011-50827030 | MS.gene73121:intron | 35.0% |
| ! | TTGAGCTAAGTTTTACGAAC+AGG | - | chr3.4:50826743-50826762 | None:intergenic | 35.0% |
| !! | TAAGCTAGAGAGTTTTCAAG+GGG | + | chr3.4:50826930-50826949 | MS.gene73121:intron | 35.0% |
| !!! | ATGTACCCATTTTTGAGTTG+GGG | + | chr3.4:50828026-50828045 | MS.gene73121:intron | 35.0% |
| !!! | CATGTACCCATTTTTGAGTT+GGG | + | chr3.4:50828025-50828044 | MS.gene73121:intron | 35.0% |
| !!! | CTTATGGTTTTGGTATACTG+TGG | + | chr3.4:50828074-50828093 | MS.gene73121:intron | 35.0% |
| !!! | GAGATTGTTGGTTTTGATGT+AGG | - | chr3.4:50826617-50826636 | None:intergenic | 35.0% |
| !!! | GGCAAGTTTTTTAGGGTTAT+CGG | - | chr3.4:50826500-50826519 | None:intergenic | 35.0% |
| AAAAGGAACCTGGAGATTGT+TGG | - | chr3.4:50826629-50826648 | None:intergenic | 40.0% | |
| AAATGCAGCTTGATAAGTGC+TGG | - | chr3.4:50828750-50828769 | None:intergenic | 40.0% | |
| AACTAGTAACCAATGCTAGC+TGG | - | chr3.4:50827866-50827885 | None:intergenic | 40.0% | |
| AGTTCTGCTAAATCAACCTG+AGG | - | chr3.4:50828726-50828745 | None:intergenic | 40.0% | |
| ATAGCCCCCAACTCAAAAAT+GGG | - | chr3.4:50828034-50828053 | None:intergenic | 40.0% | |
| CATCAAAACCAACAATCTCC+AGG | + | chr3.4:50826618-50826637 | MS.gene73121:CDS | 40.0% | |
| CTAGTTGTAGATCAGTGAGA+AGG | + | chr3.4:50827558-50827577 | MS.gene73121:intron | 40.0% | |
| GTAGAGTAGAGGGTAAGATA+TGG | + | chr3.4:50826898-50826917 | MS.gene73121:intron | 40.0% | |
| TATACTGTGGGCCTTTAACT+TGG | + | chr3.4:50828087-50828106 | MS.gene73121:intron | 40.0% | |
| TGAGAAGGGTGAAAAATGAC+AGG | + | chr3.4:50827573-50827592 | MS.gene73121:intron | 40.0% | |
| ! | CATTTTACTTGGGAAACCTC+AGG | + | chr3.4:50828707-50828726 | MS.gene73121:CDS | 40.0% |
| ! | TGTCCTGTTTTATGCGTGTT+TGG | + | chr3.4:50826590-50826609 | MS.gene73121:CDS | 40.0% |
| ! | TGTTTTTCACCAGCTAGCAT+TGG | + | chr3.4:50827854-50827873 | MS.gene73121:intron | 40.0% |
| ! | TTTTTCACTTGTGTGGTGAG+TGG | + | chr3.4:50826991-50827010 | MS.gene73121:intron | 40.0% |
| !! | GAGAAGCTCTAACACTGTTT+TGG | + | chr3.4:50827617-50827636 | MS.gene73121:intron | 40.0% |
| !!! | GCATGTACCCATTTTTGAGT+TGG | + | chr3.4:50828024-50828043 | MS.gene73121:intron | 40.0% |
| !!! | TGTACCCATTTTTGAGTTGG+GGG | + | chr3.4:50828027-50828046 | MS.gene73121:intron | 40.0% |
| !!! | TTTTGAGATTAGCCGCTATC+CGG | - | chr3.4:50827449-50827468 | None:intergenic | 40.0% |
| GATAGCCCCCAACTCAAAAA+TGG | - | chr3.4:50828035-50828054 | None:intergenic | 45.0% | |
| GATTGAAGATGTGTCTGCGT+TGG | + | chr3.4:50827108-50827127 | MS.gene73121:intron | 45.0% | |
| GGACCAAACACGCATAAAAC+AGG | - | chr3.4:50826596-50826615 | None:intergenic | 45.0% | |
| GTAGACCCTCTTTGACAACA+AGG | + | chr3.4:50828283-50828302 | MS.gene73121:intron | 45.0% | |
| GTGTTATCAATCCCGGATAG+CGG | + | chr3.4:50827434-50827453 | MS.gene73121:intron | 45.0% | |
| TCACACACACACACACACAA+AGG | - | chr3.4:50826798-50826817 | None:intergenic | 45.0% | |
| TGCTATTGCGTGTTCTGTAG+TGG | + | chr3.4:50827761-50827780 | MS.gene73121:intron | 45.0% | |
| TGTCAAAGAGGGTCTACTCA+TGG | - | chr3.4:50828280-50828299 | None:intergenic | 45.0% | |
| TGTGTGTGTGTGTGAGTTTC+AGG | + | chr3.4:50826803-50826822 | MS.gene73121:intron | 45.0% | |
| TTTCTACGTCTCAGACGAAG+TGG | - | chr3.4:50826838-50826857 | None:intergenic | 45.0% | |
| TTTGGTCCTTCATTGCTGAC+AGG | + | chr3.4:50828480-50828499 | MS.gene73121:intron | 45.0% | |
| ! | ATAAGCAAAAGTGCGGTCAC+TGG | - | chr3.4:50827359-50827378 | None:intergenic | 45.0% |
| ! | GGTCTACTCATGGTGTTGAA+TGG | - | chr3.4:50828270-50828289 | None:intergenic | 45.0% |
| ! | TTTGAGATTAGCCGCTATCC+GGG | - | chr3.4:50827448-50827467 | None:intergenic | 45.0% |
| !! | AATAGCAGAGCGGTTTGGTT+TGG | - | chr3.4:50827748-50827767 | None:intergenic | 45.0% |
| ATTGACCGACGAAGTCTCTG+AGG | - | chr3.4:50826555-50826574 | None:intergenic | 50.0% | |
| CAGAACACGCAATAGCAGAG+CGG | - | chr3.4:50827758-50827777 | None:intergenic | 50.0% | |
| GAAGAGTGAGACAGTGAGAG+TGG | + | chr3.4:50827595-50827614 | MS.gene73121:intron | 50.0% | |
| GCGTGTTCTGTAGTGGTCAA+GGG | + | chr3.4:50827768-50827787 | MS.gene73121:intron | 50.0% | |
| GTGTGTGTGTGAGTTTCAGG+TGG | + | chr3.4:50826806-50826825 | MS.gene73121:intron | 50.0% | |
| TCTACCCTCAGAGACTTCGT+CGG | + | chr3.4:50826547-50826566 | MS.gene73121:CDS | 50.0% | |
| TGCGTGTTCTGTAGTGGTCA+AGG | + | chr3.4:50827767-50827786 | MS.gene73121:intron | 50.0% | |
| TTGACCGACGAAGTCTCTGA+GGG | - | chr3.4:50826554-50826573 | None:intergenic | 50.0% | |
| ! | ACGAAGTCTCTGAGGGTAGA+AGG | - | chr3.4:50826547-50826566 | None:intergenic | 50.0% |
| ! | AGAGCGCCTTGTTGTCAAAG+AGG | - | chr3.4:50828292-50828311 | None:intergenic | 50.0% |
| ! | AGTCTCTGAGGGTAGAAGGA+AGG | - | chr3.4:50826543-50826562 | None:intergenic | 50.0% |
| ! | GAGCGCCTTGTTGTCAAAGA+GGG | - | chr3.4:50828291-50828310 | None:intergenic | 50.0% |
| CACGCAATAGCAGAGCGGTT+TGG | - | chr3.4:50827753-50827772 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 50826481 | 50828788 | 50826481 | ID=MS.gene73121 |
| chr3.4 | mRNA | 50826481 | 50828788 | 50826481 | ID=MS.gene73121.t1;Parent=MS.gene73121 |
| chr3.4 | exon | 50826481 | 50826639 | 50826481 | ID=MS.gene73121.t1.exon1;Parent=MS.gene73121.t1 |
| chr3.4 | CDS | 50826481 | 50826639 | 50826481 | ID=cds.MS.gene73121.t1;Parent=MS.gene73121.t1 |
| chr3.4 | exon | 50828660 | 50828788 | 50828660 | ID=MS.gene73121.t1.exon2;Parent=MS.gene73121.t1 |
| chr3.4 | CDS | 50828660 | 50828788 | 50828660 | ID=cds.MS.gene73121.t1;Parent=MS.gene73121.t1 |
| Gene Sequence |
| Protein sequence |
