Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86471.t1 | XP_024626767.1 | 72 | 357 | 47 | 1 | 1 | 357 | 1 | 304 | 3.90E-136 | 494.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86471.t1 | A0A2N9I863 | 58.1 | 363 | 131 | 5 | 1 | 355 | 1 | 350 | 9.8e-105 | 389.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05538 | MS.gene86471 | 0.920097 | 1.97E-87 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86471.t1 | MTR_7g062720 | 94.697 | 132 | 7 | 0 | 1 | 132 | 1 | 132 | 9.75e-88 | 261 |
| MS.gene86471.t1 | MTR_4g037355 | 70.769 | 130 | 24 | 3 | 151 | 280 | 1 | 116 | 4.19e-54 | 174 |
| MS.gene86471.t1 | MTR_5g061150 | 61.667 | 120 | 46 | 0 | 27 | 146 | 17 | 136 | 1.59e-45 | 152 |
| MS.gene86471.t1 | MTR_4g112730 | 75.000 | 64 | 16 | 0 | 138 | 201 | 1 | 64 | 2.74e-26 | 100 |
| MS.gene86471.t1 | MTR_1g036690 | 61.628 | 86 | 22 | 3 | 273 | 357 | 5 | 80 | 1.56e-23 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 66 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCCCTTAACATTTC+TGG | 0.105015 | 5.1:-15825489 | MS.gene86471:CDS |
| GCATCTCAACACAATCATTT+TGG | 0.131367 | 5.1:-15824913 | MS.gene86471:CDS |
| TTCCACTTCTGGTGGGAAAA+TGG | 0.172180 | 5.1:+15824778 | None:intergenic |
| TCTTAAGTGCTTCATTTATA+TGG | 0.216688 | 5.1:+15825337 | None:intergenic |
| ATCCAATCATATCTTGCTTT+TGG | 0.220117 | 5.1:+15825231 | None:intergenic |
| GATGCTGATTATGATATTTA+TGG | 0.234910 | 5.1:-15824559 | MS.gene86471:CDS |
| AATACTACTCCTTTGGATAT+TGG | 0.253196 | 5.1:-15824529 | MS.gene86471:CDS |
| TCCACTTCTGGTGGGAAAAT+GGG | 0.275118 | 5.1:+15824779 | None:intergenic |
| TTTGTATACTTCAGAATTAT+AGG | 0.281074 | 5.1:+15825173 | None:intergenic |
| TGTTGAGATGCATTCACTTC+AGG | 0.293409 | 5.1:+15824925 | None:intergenic |
| TGCCATAAAAGAAGGAAATA+AGG | 0.335057 | 5.1:-15825403 | MS.gene86471:CDS |
| AGTTGTTTCCAGAAATGTTA+AGG | 0.345031 | 5.1:+15825481 | None:intergenic |
| CACCAGCAGGGCGAGCTTCA+TGG | 0.368055 | 5.1:+15824959 | None:intergenic |
| CGCGGTCGTGGTCGTGGTCG+TGG | 0.386944 | 5.1:-15824859 | MS.gene86471:CDS |
| ATTGATCACCTTGTTGAAGA+TGG | 0.391237 | 5.1:-15824478 | MS.gene86471:CDS |
| GTTGTTTCCAGAAATGTTAA+GGG | 0.399743 | 5.1:+15825482 | None:intergenic |
| TAATTTAATTTCTTGTCTCC+TGG | 0.400386 | 5.1:-15825016 | MS.gene86471:CDS |
| TTTCACGGTATTGTTGTTGC+AGG | 0.408251 | 5.1:+15825058 | None:intergenic |
| CTGGAAACAACTATTTGACT+TGG | 0.414379 | 5.1:-15825470 | MS.gene86471:CDS |
| TACTTAGCTGCTGAAGGAAA+TGG | 0.418999 | 5.1:-15825429 | MS.gene86471:CDS |
| GTTGAGATGCATTCACTTCA+GGG | 0.423894 | 5.1:+15824926 | None:intergenic |
| CGCGGTCGCGGTCGTGGTCG+TGG | 0.429172 | 5.1:-15824865 | MS.gene86471:CDS |
| TAAAGGTCAACCAAATGCTT+TGG | 0.429910 | 5.1:+15824646 | None:intergenic |
| GGTCATGCCCACAATCCTAA+TGG | 0.437132 | 5.1:-15824814 | MS.gene86471:CDS |
| AACCATGAAGCTCGCCCTGC+TGG | 0.438159 | 5.1:-15824961 | MS.gene86471:CDS |
| TGACATGAATACTACTCCTT+TGG | 0.440582 | 5.1:-15824536 | MS.gene86471:CDS |
| GGGAATGGAGCTACACCAGC+AGG | 0.445287 | 5.1:+15824946 | None:intergenic |
| CGTGGTCGCGGTCGCGGTCG+TGG | 0.450743 | 5.1:-15824871 | MS.gene86471:CDS |
| CGTGGTCGTGGTCGCGGTCG+CGG | 0.452026 | 5.1:-15824877 | MS.gene86471:CDS |
| CCACTTCTGGTGGGAAAATG+GGG | 0.456004 | 5.1:+15824780 | None:intergenic |
| TCTCTCAAAACTTCACTTTG+AGG | 0.457299 | 5.1:-15825505 | MS.gene86471:CDS |
| ATGTCGTACTCCAAAGCATT+TGG | 0.465557 | 5.1:-15824656 | MS.gene86471:CDS |
| TTTGGGAGCGCTCGTGGTCG+TGG | 0.467855 | 5.1:-15824895 | MS.gene86471:CDS |
| TCCCAAAAGCAAGATATGAT+TGG | 0.468237 | 5.1:-15825233 | MS.gene86471:CDS |
| TGAAAGATCCACTTGTGCTC+TGG | 0.468533 | 5.1:-15825299 | MS.gene86471:CDS |
| CATTGTTCTGTTCAGCAACC+AGG | 0.470715 | 5.1:+15824998 | None:intergenic |
| AGCGCTCGTGGTCGTGGTCG+TGG | 0.471808 | 5.1:-15824889 | MS.gene86471:CDS |
| ACTCATTATGCTTATAAAGA+TGG | 0.483040 | 5.1:-15824586 | MS.gene86471:CDS |
| CGTGGTCGTGGTCGTGGTCG+CGG | 0.491270 | 5.1:-15824883 | MS.gene86471:CDS |
| TGCAACAACAATACCGTGAA+AGG | 0.496092 | 5.1:-15825056 | MS.gene86471:CDS |
| AAAGTATCTGTTATCGATGT+GGG | 0.505636 | 5.1:-15824702 | MS.gene86471:CDS |
| TTGTTGTTGCAGGAGCATAT+TGG | 0.511014 | 5.1:+15825068 | None:intergenic |
| AGTTTATTCCAGAGCACAAG+TGG | 0.512360 | 5.1:+15825291 | None:intergenic |
| GATGCATTCACTTCAGGGAA+TGG | 0.514758 | 5.1:+15824931 | None:intergenic |
| GCAACAACAATACCGTGAAA+GGG | 0.516930 | 5.1:-15825055 | MS.gene86471:CDS |
| GAAAAGAATGAAAATGAGAA+GGG | 0.529787 | 5.1:-15824748 | MS.gene86471:CDS |
| TAAGTACATTTCAGTATCTA+CGG | 0.532057 | 5.1:+15825446 | None:intergenic |
| TGGAAACAACTATTTGACTT+GGG | 0.534823 | 5.1:-15825469 | MS.gene86471:CDS |
| TGAAAAGAATGAAAATGAGA+AGG | 0.541748 | 5.1:-15824749 | MS.gene86471:CDS |
| AATATGCATGGTCGTGGTCG+TGG | 0.544688 | 5.1:-15824835 | MS.gene86471:CDS |
| AATGGAGATGCCATAAAAGA+AGG | 0.547076 | 5.1:-15825411 | MS.gene86471:CDS |
| GAGCATATTGGATGCATGAA+AGG | 0.556835 | 5.1:+15825080 | None:intergenic |
| AAGAATGAAAATGAGAAGGG+TGG | 0.573649 | 5.1:-15824745 | MS.gene86471:CDS |
| GAAAGTATCTGTTATCGATG+TGG | 0.574435 | 5.1:-15824703 | MS.gene86471:CDS |
| GAAATGTACTTAGCTGCTGA+AGG | 0.580070 | 5.1:-15825435 | MS.gene86471:CDS |
| ACTTCTCAATTAGAATTGTG+TGG | 0.587587 | 5.1:-15825147 | MS.gene86471:CDS |
| CAACAACAATACCGTGAAAG+GGG | 0.610582 | 5.1:-15825054 | MS.gene86471:CDS |
| AAGAAATCACCAATATCCAA+AGG | 0.617484 | 5.1:+15824520 | None:intergenic |
| AGTATCTGTTATCGATGTGG+GGG | 0.620042 | 5.1:-15824700 | MS.gene86471:CDS |
| CGTGGAAATATGCATGGTCG+TGG | 0.624916 | 5.1:-15824841 | MS.gene86471:CDS |
| CTTCATTTATATGGTGACGA+AGG | 0.627090 | 5.1:+15825346 | None:intergenic |
| AAGTATCTGTTATCGATGTG+GGG | 0.635242 | 5.1:-15824701 | MS.gene86471:CDS |
| TCACGATTCCATCTTCAACA+AGG | 0.635824 | 5.1:+15824470 | None:intergenic |
| CGTGGTCGTGGAAATATGCA+TGG | 0.646225 | 5.1:-15824847 | MS.gene86471:CDS |
| GGAATGGAGCTACACCAGCA+GGG | 0.651331 | 5.1:+15824947 | None:intergenic |
| CAAGTGGATCTTTCACAGTG+AGG | 0.718603 | 5.1:+15825307 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAAAAAACAAAGGAAAAA+TGG | - | chr5.1:15825361-15825380 | MS.gene86471:CDS | 20.0% |
| !! | TTTGTATACTTCAGAATTAT+AGG | + | chr5.1:15824805-15824824 | None:intergenic | 20.0% |
| ! | ACTCATTATGCTTATAAAGA+TGG | - | chr5.1:15825389-15825408 | MS.gene86471:CDS | 25.0% |
| ! | GAAAAGAATGAAAATGAGAA+GGG | - | chr5.1:15825227-15825246 | MS.gene86471:CDS | 25.0% |
| ! | TAAGTACATTTCAGTATCTA+CGG | + | chr5.1:15824532-15824551 | None:intergenic | 25.0% |
| ! | TCTTAAGTGCTTCATTTATA+TGG | + | chr5.1:15824641-15824660 | None:intergenic | 25.0% |
| ! | TGAAAAGAATGAAAATGAGA+AGG | - | chr5.1:15825226-15825245 | MS.gene86471:CDS | 25.0% |
| !! | GATGCTGATTATGATATTTA+TGG | - | chr5.1:15825416-15825435 | MS.gene86471:CDS | 25.0% |
| !! | TAATTTAATTTCTTGTCTCC+TGG | - | chr5.1:15824959-15824978 | MS.gene86471:CDS | 25.0% |
| !!! | TTTCATTATTTTTCCACTTC+TGG | + | chr5.1:15825211-15825230 | None:intergenic | 25.0% |
| !!! | TTTTTAGTGATTGCTGATAA+AGG | + | chr5.1:15825349-15825368 | None:intergenic | 25.0% |
| AAGAAATCACCAATATCCAA+AGG | + | chr5.1:15825458-15825477 | None:intergenic | 30.0% | |
| AATACTACTCCTTTGGATAT+TGG | - | chr5.1:15825446-15825465 | MS.gene86471:CDS | 30.0% | |
| ACTTCTCAATTAGAATTGTG+TGG | - | chr5.1:15824828-15824847 | MS.gene86471:CDS | 30.0% | |
| CAGCAATCACTAAAAAACAA+AGG | - | chr5.1:15825353-15825372 | MS.gene86471:CDS | 30.0% | |
| TGCCATAAAAGAAGGAAATA+AGG | - | chr5.1:15824572-15824591 | MS.gene86471:CDS | 30.0% | |
| ! | AAAGTATCTGTTATCGATGT+GGG | - | chr5.1:15825273-15825292 | MS.gene86471:CDS | 30.0% |
| ! | AGTTGTTTCCAGAAATGTTA+AGG | + | chr5.1:15824497-15824516 | None:intergenic | 30.0% |
| ! | ATCCAATCATATCTTGCTTT+TGG | + | chr5.1:15824747-15824766 | None:intergenic | 30.0% |
| ! | CATCTCAACACAATCATTTT+GGG | - | chr5.1:15825063-15825082 | MS.gene86471:CDS | 30.0% |
| ! | GAATTTTCCATTAGGATTGT+GGG | + | chr5.1:15825171-15825190 | None:intergenic | 30.0% |
| ! | GTTGTTTCCAGAAATGTTAA+GGG | + | chr5.1:15824496-15824515 | None:intergenic | 30.0% |
| ! | TGAATTTTCCATTAGGATTG+TGG | + | chr5.1:15825172-15825191 | None:intergenic | 30.0% |
| ! | TGGAAACAACTATTTGACTT+GGG | - | chr5.1:15824506-15824525 | MS.gene86471:CDS | 30.0% |
| !! | ATTATTTTTCCACTTCTGGT+GGG | + | chr5.1:15825207-15825226 | None:intergenic | 30.0% |
| !! | TCCAATCATATCTTGCTTTT+GGG | + | chr5.1:15824746-15824765 | None:intergenic | 30.0% |
| !!! | ATTTTTGAAATCCCCTTTCA+CGG | + | chr5.1:15824935-15824954 | None:intergenic | 30.0% |
| !!! | TGCCTTATTTCCTTCTTTTA+TGG | + | chr5.1:15824577-15824596 | None:intergenic | 30.0% |
| AAGAATGAAAATGAGAAGGG+TGG | - | chr5.1:15825230-15825249 | MS.gene86471:CDS | 35.0% | |
| AAGTATCTGTTATCGATGTG+GGG | - | chr5.1:15825274-15825293 | MS.gene86471:CDS | 35.0% | |
| AATGGAGATGCCATAAAAGA+AGG | - | chr5.1:15824564-15824583 | MS.gene86471:CDS | 35.0% | |
| ATTGATCACCTTGTTGAAGA+TGG | - | chr5.1:15825497-15825516 | MS.gene86471:CDS | 35.0% | |
| CTGGAAACAACTATTTGACT+TGG | - | chr5.1:15824505-15824524 | MS.gene86471:CDS | 35.0% | |
| TAAAGGTCAACCAAATGCTT+TGG | + | chr5.1:15825332-15825351 | None:intergenic | 35.0% | |
| TCCCAAAAGCAAGATATGAT+TGG | - | chr5.1:15824742-15824761 | MS.gene86471:CDS | 35.0% | |
| TCTCTCAAAACTTCACTTTG+AGG | - | chr5.1:15824470-15824489 | MS.gene86471:CDS | 35.0% | |
| TGACATGAATACTACTCCTT+TGG | - | chr5.1:15825439-15825458 | MS.gene86471:CDS | 35.0% | |
| ! | CTTCATTTATATGGTGACGA+AGG | + | chr5.1:15824632-15824651 | None:intergenic | 35.0% |
| ! | GCATCTCAACACAATCATTT+TGG | - | chr5.1:15825062-15825081 | MS.gene86471:CDS | 35.0% |
| !! | AATCATATCTTGCTTTTGGG+AGG | + | chr5.1:15824743-15824762 | None:intergenic | 35.0% |
| !! | CATTATTTTTCCACTTCTGG+TGG | + | chr5.1:15825208-15825227 | None:intergenic | 35.0% |
| !! | GAAAGTATCTGTTATCGATG+TGG | - | chr5.1:15825272-15825291 | MS.gene86471:CDS | 35.0% |
| !!! | TGCTTTTGCTTTTTGTTGCT+CGG | + | chr5.1:15824601-15824620 | None:intergenic | 35.0% |
| AGTATCTGTTATCGATGTGG+GGG | - | chr5.1:15825275-15825294 | MS.gene86471:CDS | 40.0% | |
| AGTTTATTCCAGAGCACAAG+TGG | + | chr5.1:15824687-15824706 | None:intergenic | 40.0% | |
| CAACAACAATACCGTGAAAG+GGG | - | chr5.1:15824921-15824940 | MS.gene86471:CDS | 40.0% | |
| GAAATGTACTTAGCTGCTGA+AGG | - | chr5.1:15824540-15824559 | MS.gene86471:CDS | 40.0% | |
| GAGCATATTGGATGCATGAA+AGG | + | chr5.1:15824898-15824917 | None:intergenic | 40.0% | |
| GCAACAACAATACCGTGAAA+GGG | - | chr5.1:15824920-15824939 | MS.gene86471:CDS | 40.0% | |
| GTTGAGATGCATTCACTTCA+GGG | + | chr5.1:15825052-15825071 | None:intergenic | 40.0% | |
| TACTTAGCTGCTGAAGGAAA+TGG | - | chr5.1:15824546-15824565 | MS.gene86471:CDS | 40.0% | |
| TCACGATTCCATCTTCAACA+AGG | + | chr5.1:15825508-15825527 | None:intergenic | 40.0% | |
| TGCAACAACAATACCGTGAA+AGG | - | chr5.1:15824919-15824938 | MS.gene86471:CDS | 40.0% | |
| TGTTGAGATGCATTCACTTC+AGG | + | chr5.1:15825053-15825072 | None:intergenic | 40.0% | |
| ! | ATGTCGTACTCCAAAGCATT+TGG | - | chr5.1:15825319-15825338 | MS.gene86471:CDS | 40.0% |
| ! | TTGTTGTTGCAGGAGCATAT+TGG | + | chr5.1:15824910-15824929 | None:intergenic | 40.0% |
| ! | TTTCACGGTATTGTTGTTGC+AGG | + | chr5.1:15824920-15824939 | None:intergenic | 40.0% |
| ! | TTTGAGGCCCTTAACATTTC+TGG | - | chr5.1:15824486-15824505 | MS.gene86471:CDS | 40.0% |
| !! | TCTTGCTTTTGGGAGGATAA+TGG | + | chr5.1:15824736-15824755 | None:intergenic | 40.0% |
| !!! | GGGGTTCTGAATTTTCCATT+AGG | + | chr5.1:15825179-15825198 | None:intergenic | 40.0% |
| CAAGTGGATCTTTCACAGTG+AGG | + | chr5.1:15824671-15824690 | None:intergenic | 45.0% | |
| CATTGTTCTGTTCAGCAACC+AGG | + | chr5.1:15824980-15824999 | None:intergenic | 45.0% | |
| GATGCATTCACTTCAGGGAA+TGG | + | chr5.1:15825047-15825066 | None:intergenic | 45.0% | |
| TCCACTTCTGGTGGGAAAAT+GGG | + | chr5.1:15825199-15825218 | None:intergenic | 45.0% | |
| TGAAAGATCCACTTGTGCTC+TGG | - | chr5.1:15824676-15824695 | MS.gene86471:CDS | 45.0% | |
| TGTTATCGATGTGGGGGAAA+AGG | - | chr5.1:15825281-15825300 | MS.gene86471:CDS | 45.0% | |
| TTCCACTTCTGGTGGGAAAA+TGG | + | chr5.1:15825200-15825219 | None:intergenic | 45.0% | |
| AATATGCATGGTCGTGGTCG+TGG | - | chr5.1:15825140-15825159 | MS.gene86471:CDS | 50.0% | |
| CCACTTCTGGTGGGAAAATG+GGG | + | chr5.1:15825198-15825217 | None:intergenic | 50.0% | |
| CGTGGAAATATGCATGGTCG+TGG | - | chr5.1:15825134-15825153 | MS.gene86471:CDS | 50.0% | |
| CGTGGTCGTGGAAATATGCA+TGG | - | chr5.1:15825128-15825147 | MS.gene86471:CDS | 50.0% | |
| GATGTGGGGGAAAAGGTCAT+TGG | - | chr5.1:15825288-15825307 | MS.gene86471:CDS | 50.0% | |
| GGTCATGCCCACAATCCTAA+TGG | - | chr5.1:15825161-15825180 | MS.gene86471:CDS | 50.0% | |
| !!! | AATCATTTTGGGAGCGCTCG+TGG | - | chr5.1:15825074-15825093 | MS.gene86471:CDS | 50.0% |
| GGAATGGAGCTACACCAGCA+GGG | + | chr5.1:15825031-15825050 | None:intergenic | 55.0% | |
| ! | CCCCATTTTCCCACCAGAAG+TGG | - | chr5.1:15825195-15825214 | MS.gene86471:CDS | 55.0% |
| AACCATGAAGCTCGCCCTGC+TGG | - | chr5.1:15825014-15825033 | MS.gene86471:CDS | 60.0% | |
| GGGAATGGAGCTACACCAGC+AGG | + | chr5.1:15825032-15825051 | None:intergenic | 60.0% | |
| CACCAGCAGGGCGAGCTTCA+TGG | + | chr5.1:15825019-15825038 | None:intergenic | 65.0% | |
| !! | TTTGGGAGCGCTCGTGGTCG+TGG | - | chr5.1:15825080-15825099 | MS.gene86471:CDS | 65.0% |
| AGCGCTCGTGGTCGTGGTCG+TGG | - | chr5.1:15825086-15825105 | MS.gene86471:CDS | 70.0% | |
| CGTGGTCGTGGTCGTGGTCG+CGG | - | chr5.1:15825092-15825111 | MS.gene86471:CDS | 70.0% | |
| CGCGGTCGTGGTCGTGGTCG+TGG | - | chr5.1:15825116-15825135 | MS.gene86471:CDS | 75.0% | |
| CGTGGTCGTGGTCGCGGTCG+CGG | - | chr5.1:15825098-15825117 | MS.gene86471:CDS | 75.0% | |
| ! | CGCGGTCGCGGTCGTGGTCG+TGG | - | chr5.1:15825110-15825129 | MS.gene86471:CDS | 80.0% |
| ! | CGTGGTCGCGGTCGCGGTCG+TGG | - | chr5.1:15825104-15825123 | MS.gene86471:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 15824462 | 15825535 | 15824462 | ID=MS.gene86471 |
| chr5.1 | mRNA | 15824462 | 15825535 | 15824462 | ID=MS.gene86471.t1;Parent=MS.gene86471 |
| chr5.1 | exon | 15824462 | 15825535 | 15824462 | ID=MS.gene86471.t1.exon1;Parent=MS.gene86471.t1 |
| chr5.1 | CDS | 15824462 | 15825535 | 15824462 | ID=cds.MS.gene86471.t1;Parent=MS.gene86471.t1 |
| Gene Sequence |
| Protein sequence |
