AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05538


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05538.t1 MTR_7g062720 94.697 132 7 0 1 132 1 132 1.06e-87 261
MS.gene05538.t1 MTR_4g037355 70.769 130 24 3 151 280 1 116 1.28e-53 173
MS.gene05538.t1 MTR_5g061150 61.667 120 46 0 27 146 17 136 1.53e-45 152
MS.gene05538.t1 MTR_4g112730 75.000 64 16 0 138 201 1 64 2.74e-26 100
MS.gene05538.t1 MTR_1g036690 61.628 86 22 3 273 357 5 80 1.42e-23 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 62 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCCCTTAACATTTC+TGG 0.105015 6.2:-84857462 MS.gene05538:CDS
GCATCTCAACACAATCATTT+TGG 0.131367 6.2:-84856886 MS.gene05538:CDS
TTCCACTTCTGGTGGGAAAA+TGG 0.172180 6.2:+84856751 None:intergenic
GATGCTGATTATGATATTTA+TGG 0.197094 6.2:-84856532 MS.gene05538:CDS
TCTTAAGTGCTTCATTTATA+TGG 0.216688 6.2:+84857310 None:intergenic
ATCCAATCATATCTTGCTTT+TGG 0.220117 6.2:+84857204 None:intergenic
AATACTACTCCTTTGGATAT+TGG 0.253196 6.2:-84856502 MS.gene05538:CDS
TCCACTTCTGGTGGGAAAAT+GGG 0.275118 6.2:+84856752 None:intergenic
TTTGTATACTTCAGAATTAT+AGG 0.281074 6.2:+84857146 None:intergenic
TGTTGAGATGCATTCACTTC+AGG 0.293409 6.2:+84856898 None:intergenic
TGCCATAAAAGAAGGAAATA+AGG 0.335057 6.2:-84857376 MS.gene05538:CDS
AGTTGTTTCCAGAAATGTTA+AGG 0.345031 6.2:+84857454 None:intergenic
TAATTTAATTTCTTGTCTCT+TGG 0.356568 6.2:-84856989 MS.gene05538:CDS
CACCAGCAGGGCGAGCTTCA+TGG 0.368055 6.2:+84856932 None:intergenic
CGCGGTCGTGGTCGTGGTCG+TGG 0.386944 6.2:-84856832 MS.gene05538:CDS
ATTGATCACCTTGTTGAAGA+TGG 0.391237 6.2:-84856451 MS.gene05538:CDS
GTTGTTTCCAGAAATGTTAA+GGG 0.399743 6.2:+84857455 None:intergenic
TTTCACGGTATTGTTGTTGC+AGG 0.408251 6.2:+84857031 None:intergenic
CTGGAAACAACTATTTGACT+TGG 0.414379 6.2:-84857443 MS.gene05538:CDS
TACTTAGCTGCTGAAGGAAA+TGG 0.418999 6.2:-84857402 MS.gene05538:CDS
GTTGAGATGCATTCACTTCA+GGG 0.423894 6.2:+84856899 None:intergenic
CGCGGTCGCGGTCGTGGTCG+TGG 0.429172 6.2:-84856838 MS.gene05538:CDS
TAAAGGTCAACCAAATGCTT+TGG 0.429910 6.2:+84856619 None:intergenic
GGTCATGCCCACAATCCTAA+TGG 0.437132 6.2:-84856787 MS.gene05538:CDS
AACCATGAAGCTCGCCCTGC+TGG 0.438159 6.2:-84856934 MS.gene05538:CDS
TGACATGAATACTACTCCTT+TGG 0.440582 6.2:-84856509 MS.gene05538:CDS
GGGAATGGAGCTACACCAGC+AGG 0.445287 6.2:+84856919 None:intergenic
CGTGGTCGCGGTCGCGGTCG+TGG 0.450743 6.2:-84856844 MS.gene05538:CDS
CGTGGTCGTGGTCGCGGTCG+CGG 0.452026 6.2:-84856850 MS.gene05538:CDS
CCACTTCTGGTGGGAAAATG+GGG 0.456004 6.2:+84856753 None:intergenic
TCTCTCAAAACTTCACTTTG+AGG 0.457299 6.2:-84857478 MS.gene05538:CDS
ATGTCGTACTCCAAAGCATT+TGG 0.465557 6.2:-84856629 MS.gene05538:CDS
TTTGGGAGCGCTCGTGGTCG+TGG 0.467855 6.2:-84856868 MS.gene05538:CDS
TCCCAAAAGCAAGATATGAT+TGG 0.468237 6.2:-84857206 MS.gene05538:CDS
TGAAAGATCCACTTGTGCTC+TGG 0.468533 6.2:-84857272 MS.gene05538:CDS
AGCGCTCGTGGTCGTGGTCG+TGG 0.471808 6.2:-84856862 MS.gene05538:CDS
ACTCATTATGCTTATAAAGA+TGG 0.476220 6.2:-84856559 MS.gene05538:CDS
CGTGGTCGTGGTCGTGGTCG+CGG 0.491270 6.2:-84856856 MS.gene05538:CDS
TGCAACAACAATACCGTGAA+AGG 0.496092 6.2:-84857029 MS.gene05538:CDS
AAAGTATCTGTTATCGATGT+GGG 0.505636 6.2:-84856675 MS.gene05538:CDS
TTGTTGTTGCAGGAGCATAT+TGG 0.511014 6.2:+84857041 None:intergenic
AGTTTATTCCAGAGCACAAG+TGG 0.512360 6.2:+84857264 None:intergenic
GATGCATTCACTTCAGGGAA+TGG 0.514758 6.2:+84856904 None:intergenic
GCAACAACAATACCGTGAAA+GGG 0.516930 6.2:-84857028 MS.gene05538:CDS
TAAGTACATTTCAGTATCTA+CGG 0.532057 6.2:+84857419 None:intergenic
TGGAAACAACTATTTGACTT+GGG 0.534823 6.2:-84857442 MS.gene05538:CDS
AATATGCATGGTCGTGGTCG+TGG 0.544688 6.2:-84856808 MS.gene05538:CDS
AATGGAGATGCCATAAAAGA+AGG 0.547076 6.2:-84857384 MS.gene05538:CDS
GAGCATATTGGATGCATGAA+AGG 0.556835 6.2:+84857053 None:intergenic
GAAAGTATCTGTTATCGATG+TGG 0.574435 6.2:-84856676 MS.gene05538:CDS
GAAATGTACTTAGCTGCTGA+AGG 0.580070 6.2:-84857408 MS.gene05538:CDS
ACTTCTCAATTAGAATTGTG+TGG 0.587587 6.2:-84857120 MS.gene05538:CDS
CAACAACAATACCGTGAAAG+GGG 0.610582 6.2:-84857027 MS.gene05538:CDS
AAGAAATCACCAATATCCAA+AGG 0.617484 6.2:+84856493 None:intergenic
AGTATCTGTTATCGATGTGG+GGG 0.620042 6.2:-84856673 MS.gene05538:CDS
CGTGGAAATATGCATGGTCG+TGG 0.624916 6.2:-84856814 MS.gene05538:CDS
CTTCATTTATATGGTGACGA+AGG 0.627090 6.2:+84857319 None:intergenic
AAGTATCTGTTATCGATGTG+GGG 0.635242 6.2:-84856674 MS.gene05538:CDS
TCACGATTCCATCTTCAACA+AGG 0.635824 6.2:+84856443 None:intergenic
CGTGGTCGTGGAAATATGCA+TGG 0.646225 6.2:-84856820 MS.gene05538:CDS
GGAATGGAGCTACACCAGCA+GGG 0.651331 6.2:+84856920 None:intergenic
CAAGTGGATCTTTCACAGTG+AGG 0.718603 6.2:+84857280 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAAAAAACAAAGGAAAAA+TGG - chr6.2:84857334-84857353 MS.gene05538:CDS 20.0%
!! GAAAAAAATGAAAATGAGAA+GGG - chr6.2:84857200-84857219 MS.gene05538:CDS 20.0%
!! TGAAAAAAATGAAAATGAGA+AGG - chr6.2:84857199-84857218 MS.gene05538:CDS 20.0%
!! TTTGTATACTTCAGAATTAT+AGG + chr6.2:84856778-84856797 None:intergenic 20.0%
!!! TAATTTAATTTCTTGTCTCT+TGG - chr6.2:84856932-84856951 MS.gene05538:CDS 20.0%
! ACTCATTATGCTTATAAAGA+TGG - chr6.2:84857362-84857381 MS.gene05538:CDS 25.0%
! TAAGTACATTTCAGTATCTA+CGG + chr6.2:84856505-84856524 None:intergenic 25.0%
! TCTTAAGTGCTTCATTTATA+TGG + chr6.2:84856614-84856633 None:intergenic 25.0%
!! GATGCTGATTATGATATTTA+TGG - chr6.2:84857389-84857408 MS.gene05538:CDS 25.0%
!!! TTTCATTATTTTTCCACTTC+TGG + chr6.2:84857184-84857203 None:intergenic 25.0%
!!! TTTTTAGTGATTGCTGATAA+AGG + chr6.2:84857322-84857341 None:intergenic 25.0%
AAAAATGAAAATGAGAAGGG+TGG - chr6.2:84857203-84857222 MS.gene05538:CDS 30.0%
AAGAAATCACCAATATCCAA+AGG + chr6.2:84857431-84857450 None:intergenic 30.0%
AATACTACTCCTTTGGATAT+TGG - chr6.2:84857419-84857438 MS.gene05538:CDS 30.0%
ACTTCTCAATTAGAATTGTG+TGG - chr6.2:84856801-84856820 MS.gene05538:CDS 30.0%
CAGCAATCACTAAAAAACAA+AGG - chr6.2:84857326-84857345 MS.gene05538:CDS 30.0%
TGCCATAAAAGAAGGAAATA+AGG - chr6.2:84856545-84856564 MS.gene05538:CDS 30.0%
TGGTGAAAAAATAGCAGATA+CGG - chr6.2:84856821-84856840 MS.gene05538:CDS 30.0%
! AAAGTATCTGTTATCGATGT+GGG - chr6.2:84857246-84857265 MS.gene05538:CDS 30.0%
! AGTTGTTTCCAGAAATGTTA+AGG + chr6.2:84856470-84856489 None:intergenic 30.0%
! ATCCAATCATATCTTGCTTT+TGG + chr6.2:84856720-84856739 None:intergenic 30.0%
! CATCTCAACACAATCATTTT+GGG - chr6.2:84857036-84857055 MS.gene05538:CDS 30.0%
! GAATTTTCCATTAGGATTGT+GGG + chr6.2:84857144-84857163 None:intergenic 30.0%
! GTTGTTTCCAGAAATGTTAA+GGG + chr6.2:84856469-84856488 None:intergenic 30.0%
! TGAATTTTCCATTAGGATTG+TGG + chr6.2:84857145-84857164 None:intergenic 30.0%
! TGGAAACAACTATTTGACTT+GGG - chr6.2:84856479-84856498 MS.gene05538:CDS 30.0%
!! ATTATTTTTCCACTTCTGGT+GGG + chr6.2:84857180-84857199 None:intergenic 30.0%
!! TCCAATCATATCTTGCTTTT+GGG + chr6.2:84856719-84856738 None:intergenic 30.0%
!!! ATTTTTGAAATCCCCTTTCA+CGG + chr6.2:84856908-84856927 None:intergenic 30.0%
!!! TGCCTTATTTCCTTCTTTTA+TGG + chr6.2:84856550-84856569 None:intergenic 30.0%
AAGTATCTGTTATCGATGTG+GGG - chr6.2:84857247-84857266 MS.gene05538:CDS 35.0%
AATGGAGATGCCATAAAAGA+AGG - chr6.2:84856537-84856556 MS.gene05538:CDS 35.0%
ATTGATCACCTTGTTGAAGA+TGG - chr6.2:84857470-84857489 MS.gene05538:CDS 35.0%
CTGGAAACAACTATTTGACT+TGG - chr6.2:84856478-84856497 MS.gene05538:CDS 35.0%
TAAAGGTCAACCAAATGCTT+TGG + chr6.2:84857305-84857324 None:intergenic 35.0%
TCCCAAAAGCAAGATATGAT+TGG - chr6.2:84856715-84856734 MS.gene05538:CDS 35.0%
TCTCTCAAAACTTCACTTTG+AGG - chr6.2:84856443-84856462 MS.gene05538:CDS 35.0%
TGACATGAATACTACTCCTT+TGG - chr6.2:84857412-84857431 MS.gene05538:CDS 35.0%
! CTTCATTTATATGGTGACGA+AGG + chr6.2:84856605-84856624 None:intergenic 35.0%
! GCATCTCAACACAATCATTT+TGG - chr6.2:84857035-84857054 MS.gene05538:CDS 35.0%
!! AATCATATCTTGCTTTTGGG+AGG + chr6.2:84856716-84856735 None:intergenic 35.0%
!! CATTATTTTTCCACTTCTGG+TGG + chr6.2:84857181-84857200 None:intergenic 35.0%
!! GAAAGTATCTGTTATCGATG+TGG - chr6.2:84857245-84857264 MS.gene05538:CDS 35.0%
!!! TGCTTTTGCTTTTTGTTGCT+CGG + chr6.2:84856574-84856593 None:intergenic 35.0%
AGTATCTGTTATCGATGTGG+GGG - chr6.2:84857248-84857267 MS.gene05538:CDS 40.0%
AGTTTATTCCAGAGCACAAG+TGG + chr6.2:84856660-84856679 None:intergenic 40.0%
CAACAACAATACCGTGAAAG+GGG - chr6.2:84856894-84856913 MS.gene05538:CDS 40.0%
GAAATGTACTTAGCTGCTGA+AGG - chr6.2:84856513-84856532 MS.gene05538:CDS 40.0%
GAGCATATTGGATGCATGAA+AGG + chr6.2:84856871-84856890 None:intergenic 40.0%
GCAACAACAATACCGTGAAA+GGG - chr6.2:84856893-84856912 MS.gene05538:CDS 40.0%
GTTGAGATGCATTCACTTCA+GGG + chr6.2:84857025-84857044 None:intergenic 40.0%
TACTTAGCTGCTGAAGGAAA+TGG - chr6.2:84856519-84856538 MS.gene05538:CDS 40.0%
TCACGATTCCATCTTCAACA+AGG + chr6.2:84857481-84857500 None:intergenic 40.0%
TGCAACAACAATACCGTGAA+AGG - chr6.2:84856892-84856911 MS.gene05538:CDS 40.0%
TGTTGAGATGCATTCACTTC+AGG + chr6.2:84857026-84857045 None:intergenic 40.0%
! ATGTCGTACTCCAAAGCATT+TGG - chr6.2:84857292-84857311 MS.gene05538:CDS 40.0%
! TTGTTGTTGCAGGAGCATAT+TGG + chr6.2:84856883-84856902 None:intergenic 40.0%
! TTTCACGGTATTGTTGTTGC+AGG + chr6.2:84856893-84856912 None:intergenic 40.0%
! TTTGAGGCCCTTAACATTTC+TGG - chr6.2:84856459-84856478 MS.gene05538:CDS 40.0%
!! TCTTGCTTTTGGGAGGATAA+TGG + chr6.2:84856709-84856728 None:intergenic 40.0%
!!! GGGGTTCTGAATTTTCCATT+AGG + chr6.2:84857152-84857171 None:intergenic 40.0%
CAAGTGGATCTTTCACAGTG+AGG + chr6.2:84856644-84856663 None:intergenic 45.0%
GATGCATTCACTTCAGGGAA+TGG + chr6.2:84857020-84857039 None:intergenic 45.0%
TCCACTTCTGGTGGGAAAAT+GGG + chr6.2:84857172-84857191 None:intergenic 45.0%
TGAAAGATCCACTTGTGCTC+TGG - chr6.2:84856649-84856668 MS.gene05538:CDS 45.0%
TGTTATCGATGTGGGGGAAA+AGG - chr6.2:84857254-84857273 MS.gene05538:CDS 45.0%
TTCCACTTCTGGTGGGAAAA+TGG + chr6.2:84857173-84857192 None:intergenic 45.0%
AATATGCATGGTCGTGGTCG+TGG - chr6.2:84857113-84857132 MS.gene05538:CDS 50.0%
CCACTTCTGGTGGGAAAATG+GGG + chr6.2:84857171-84857190 None:intergenic 50.0%
CGTGGAAATATGCATGGTCG+TGG - chr6.2:84857107-84857126 MS.gene05538:CDS 50.0%
CGTGGTCGTGGAAATATGCA+TGG - chr6.2:84857101-84857120 MS.gene05538:CDS 50.0%
GATGTGGGGGAAAAGGTCAT+TGG - chr6.2:84857261-84857280 MS.gene05538:CDS 50.0%
GGTCATGCCCACAATCCTAA+TGG - chr6.2:84857134-84857153 MS.gene05538:CDS 50.0%
!!! AATCATTTTGGGAGCGCTCG+TGG - chr6.2:84857047-84857066 MS.gene05538:CDS 50.0%
GGAATGGAGCTACACCAGCA+GGG + chr6.2:84857004-84857023 None:intergenic 55.0%
! CCCCATTTTCCCACCAGAAG+TGG - chr6.2:84857168-84857187 MS.gene05538:CDS 55.0%
AACCATGAAGCTCGCCCTGC+TGG - chr6.2:84856987-84857006 MS.gene05538:CDS 60.0%
GGGAATGGAGCTACACCAGC+AGG + chr6.2:84857005-84857024 None:intergenic 60.0%
CACCAGCAGGGCGAGCTTCA+TGG + chr6.2:84856992-84857011 None:intergenic 65.0%
!! TTTGGGAGCGCTCGTGGTCG+TGG - chr6.2:84857053-84857072 MS.gene05538:CDS 65.0%
AGCGCTCGTGGTCGTGGTCG+TGG - chr6.2:84857059-84857078 MS.gene05538:CDS 70.0%
CGTGGTCGTGGTCGTGGTCG+CGG - chr6.2:84857065-84857084 MS.gene05538:CDS 70.0%
CGCGGTCGTGGTCGTGGTCG+TGG - chr6.2:84857089-84857108 MS.gene05538:CDS 75.0%
CGTGGTCGTGGTCGCGGTCG+CGG - chr6.2:84857071-84857090 MS.gene05538:CDS 75.0%
! CGCGGTCGCGGTCGTGGTCG+TGG - chr6.2:84857083-84857102 MS.gene05538:CDS 80.0%
! CGTGGTCGCGGTCGCGGTCG+TGG - chr6.2:84857077-84857096 MS.gene05538:CDS 80.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 84856435 84857508 84856435 ID=MS.gene05538
chr6.2 mRNA 84856435 84857508 84856435 ID=MS.gene05538.t1;Parent=MS.gene05538
chr6.2 exon 84856435 84857508 84856435 ID=MS.gene05538.t1.exon1;Parent=MS.gene05538.t1
chr6.2 CDS 84856435 84857508 84856435 ID=cds.MS.gene05538.t1;Parent=MS.gene05538.t1
Gene Sequence

>MS.gene05538

ATGTCAAATCTCTCAAAACTTCACTTTGAGGCCCTTAACATTTCTGGAAACAACTATTTGACTTGGGCCGTAGATACTGAAATGTACTTAGCTGCTGAAGGAAATGGAGATGCCATAAAAGAAGGAAATAAGGCATCCGAGCAACAAAAAGCAAAAGCATTAATATTCCTTCGTCACCATATAAATGAAGCACTTAAGAATGAATACCTCACTGTGAAAGATCCACTTGTGCTCTGGAATAAACTAAAAGAAAGATATGAGCACTTGAAAGCCATTATCCTCCCAAAAGCAAGATATGATTGGATGCACTTGCGCTTACAAGACTTTAAGTCTGTAACTGCCTATAATTCTGAAGTATACAAAATTACTTCTCAATTAGAATTGTGTGGTGAAAAAATAGCAGATACGGATCTGTTAGAGAAAACATTTTCAACCTTTCATGCATCCAATATGCTCCTGCAACAACAATACCGTGAAAGGGGATTTCAAAAATACTCTAATTTAATTTCTTGTCTCTTGGTTGCTGAACAGAACAATGAGCTTCTAATGAAAAACCATGAAGCTCGCCCTGCTGGTGTAGCTCCATTCCCTGAAGTGAATGCATCTCAACACAATCATTTTGGGAGCGCTCGTGGTCGTGGTCGTGGTCGCGGTCGCGGTCGTGGTCGTGGTCGTGGAAATATGCATGGTCGTGGTCGTGGTCATGCCCACAATCCTAATGGAAAATTCAGAACCCCATTTTCCCACCAGAAGTGGAAAAATAATGAAAAAAATGAAAATGAGAAGGGTGGACAAAGTAGCAAAAGAAATGAAAGTATCTGTTATCGATGTGGGGGAAAAGGTCATTGGTCTCGTACATGTCGTACTCCAAAGCATTTGGTTGACCTTTATCAGCAATCACTAAAAAACAAAGGAAAAATGGTTGAAACTCATTATGCTTATAAAGATGGTAATGATGCTGATTATGATATTTATGGTGACATGAATACTACTCCTTTGGATATTGGTGATTTCTTTGAAAATCAAAATGAAAAAATTGATCACCTTGTTGAAGATGGAATCGTGAAGAAATAG

Protein sequence

>MS.gene05538.t1

MSNLSKLHFEALNISGNNYLTWAVDTEMYLAAEGNGDAIKEGNKASEQQKAKALIFLRHHINEALKNEYLTVKDPLVLWNKLKERYEHLKAIILPKARYDWMHLRLQDFKSVTAYNSEVYKITSQLELCGEKIADTDLLEKTFSTFHASNMLLQQQYRERGFQKYSNLISCLLVAEQNNELLMKNHEARPAGVAPFPEVNASQHNHFGSARGRGRGRGRGRGRGRGNMHGRGRGHAHNPNGKFRTPFSHQKWKNNEKNENEKGGQSSKRNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLKNKGKMVETHYAYKDGNDADYDIYGDMNTTPLDIGDFFENQNEKIDHLVEDGIVKK