Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86512.t1 | AES95383.1 | 97.3 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 1.30E-72 | 282.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86512.t1 | G7K2M0 | 97.3 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 9.2e-73 | 282.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051201 | MS.gene86512 | 0.810068 | 1.39E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86512.t1 | MTR_5g024910 | 97.333 | 150 | 3 | 1 | 1 | 150 | 1 | 149 | 2.93e-103 | 292 |
| MS.gene86512.t1 | MTR_8g072610 | 61.871 | 139 | 37 | 3 | 1 | 138 | 1 | 124 | 2.80e-48 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAACATCTATTTCTTCTA+TGG | 0.231886 | 5.1:-15435863 | MS.gene86512:CDS |
| TCATGAATATGTACTACAAT+TGG | 0.324853 | 5.1:+15435826 | None:intergenic |
| TAACTCTGTTGTAACTCATT+TGG | 0.334667 | 5.1:+15436235 | None:intergenic |
| ATGGAAAATGCAGAGGATTT+AGG | 0.352171 | 5.1:-15436170 | MS.gene86512:CDS |
| AGAAGCTATTGCAGATTGTT+TGG | 0.378114 | 5.1:-15435896 | MS.gene86512:CDS |
| AATAGCAATAGTAGTAGAAA+TGG | 0.412385 | 5.1:-15436000 | MS.gene86512:CDS |
| CATGAATATGTACTACAATT+GGG | 0.470108 | 5.1:+15435827 | None:intergenic |
| ACCAGCAGCAGTAGTGGTAA+AGG | 0.475843 | 5.1:-15436207 | MS.gene86512:CDS |
| TCCTTTACCACTACTGCTGC+TGG | 0.481982 | 5.1:+15436206 | None:intergenic |
| AATGAAGGATGAAAGAATCA+AGG | 0.492289 | 5.1:-15435974 | MS.gene86512:CDS |
| GAAAGAATCAAGGGTCAAGC+AGG | 0.515043 | 5.1:-15435964 | MS.gene86512:CDS |
| AGCAGCACCAGCAGCAGTAG+TGG | 0.516312 | 5.1:-15436213 | MS.gene86512:CDS |
| TAGTAGAAATGGTTTAATGA+AGG | 0.548221 | 5.1:-15435989 | MS.gene86512:CDS |
| TCAACTTATCAAAGATTCTG+AGG | 0.566527 | 5.1:+15436094 | None:intergenic |
| ATGAAGGATGAAAGAATCAA+GGG | 0.587644 | 5.1:-15435973 | MS.gene86512:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAGCAATAGTAGTAGAAA+TGG | - | chr5.1:15436050-15436069 | MS.gene86512:CDS | 25.0% |
| ! | CATGAATATGTACTACAATT+GGG | + | chr5.1:15436226-15436245 | None:intergenic | 25.0% |
| ! | TAGTAGAAATGGTTTAATGA+AGG | - | chr5.1:15436061-15436080 | MS.gene86512:CDS | 25.0% |
| ! | TCATGAATATGTACTACAAT+TGG | + | chr5.1:15436227-15436246 | None:intergenic | 25.0% |
| !! | AAGAACATCTATTTCTTCTA+TGG | - | chr5.1:15436187-15436206 | MS.gene86512:CDS | 25.0% |
| !! | GATTGTAGATTTTCATCTTA+TGG | - | chr5.1:15436110-15436129 | MS.gene86512:CDS | 25.0% |
| AATGAAGGATGAAAGAATCA+AGG | - | chr5.1:15436076-15436095 | MS.gene86512:CDS | 30.0% | |
| ATGAAGGATGAAAGAATCAA+GGG | - | chr5.1:15436077-15436096 | MS.gene86512:CDS | 30.0% | |
| TAACTCTGTTGTAACTCATT+TGG | + | chr5.1:15435818-15435837 | None:intergenic | 30.0% | |
| TCAACTTATCAAAGATTCTG+AGG | + | chr5.1:15435959-15435978 | None:intergenic | 30.0% | |
| ! | AAAGGATCATCATTTTACCA+TGG | - | chr5.1:15435861-15435880 | MS.gene86512:CDS | 30.0% |
| ! | AAAGTTTTCTGCAGAAAAAG+TGG | - | chr5.1:15436014-15436033 | MS.gene86512:CDS | 30.0% |
| ! | GTAGATTTTCATCTTATGGA+AGG | - | chr5.1:15436114-15436133 | MS.gene86512:CDS | 30.0% |
| !!! | TCACAAGACTTTACTTTTTG+CGG | - | chr5.1:15435910-15435929 | MS.gene86512:CDS | 30.0% |
| AGAAGCTATTGCAGATTGTT+TGG | - | chr5.1:15436154-15436173 | MS.gene86512:CDS | 35.0% | |
| ATGGAAAATGCAGAGGATTT+AGG | - | chr5.1:15435880-15435899 | MS.gene86512:CDS | 35.0% | |
| ! | GCAAAAAGTAAAGTCTTGTG+AGG | + | chr5.1:15435911-15435930 | None:intergenic | 35.0% |
| ! | TAAATCCTCTGCATTTTCCA+TGG | + | chr5.1:15435881-15435900 | None:intergenic | 35.0% |
| ! | TTTTACCATGGAAAATGCAG+AGG | - | chr5.1:15435873-15435892 | MS.gene86512:CDS | 35.0% |
| GAAAGAATCAAGGGTCAAGC+AGG | - | chr5.1:15436086-15436105 | MS.gene86512:CDS | 45.0% | |
| ACCAGCAGCAGTAGTGGTAA+AGG | - | chr5.1:15435843-15435862 | MS.gene86512:CDS | 50.0% | |
| TCCTTTACCACTACTGCTGC+TGG | + | chr5.1:15435847-15435866 | None:intergenic | 50.0% | |
| AGCAGCACCAGCAGCAGTAG+TGG | - | chr5.1:15435837-15435856 | MS.gene86512:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 15435810 | 15436262 | 15435810 | ID=MS.gene86512 |
| chr5.1 | mRNA | 15435810 | 15436262 | 15435810 | ID=MS.gene86512.t1;Parent=MS.gene86512 |
| chr5.1 | exon | 15435810 | 15436262 | 15435810 | ID=MS.gene86512.t1.exon1;Parent=MS.gene86512.t1 |
| chr5.1 | CDS | 15435810 | 15436262 | 15435810 | ID=cds.MS.gene86512.t1;Parent=MS.gene86512.t1 |
| Gene Sequence |
| Protein sequence |
