Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86558.t1 | XP_003612350.1 | 93.1 | 145 | 10 | 0 | 1 | 145 | 186 | 330 | 2.30E-66 | 261.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86558.t1 | O81791 | 67.5 | 126 | 41 | 0 | 3 | 128 | 229 | 354 | 7.4e-42 | 171.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86558.t1 | G7K041 | 93.1 | 145 | 10 | 0 | 1 | 145 | 186 | 330 | 1.6e-66 | 261.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058906 | MS.gene86558 | 0.807952 | 3.93E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGGAAGTGCAGAGAATT+TGG | 0.277802 | 5.1:-14752136 | MS.gene86558:CDS |
| AAAGATTACTTTAACAGATT+TGG | 0.286068 | 5.1:-14752224 | MS.gene86558:CDS |
| CAGTCTCATTCATGATCTTC+AGG | 0.329225 | 5.1:-14752074 | MS.gene86558:intron |
| TTCTTTGACGCTTCAGCAAT+TGG | 0.353848 | 5.1:+14752184 | None:intergenic |
| GTATTCCTCGCTGGCCTCAT+AGG | 0.379146 | 5.1:-14752114 | MS.gene86558:CDS |
| AGATGCTAATTGCTAGGTTT+AGG | 0.384292 | 5.1:+14751310 | None:intergenic |
| CATAGAGAAGAGGCTCTTCA+TGG | 0.402183 | 5.1:-14751428 | MS.gene86558:CDS |
| CGTCAAAGAATCTAAATGTT+GGG | 0.449206 | 5.1:-14752171 | MS.gene86558:CDS |
| TTGCCTTCTTATCACTGCCA+TGG | 0.457172 | 5.1:+14751477 | None:intergenic |
| AAAGATGTTGGAAAGTGAAA+AGG | 0.459498 | 5.1:-14751455 | MS.gene86558:CDS |
| TTGGGCTTACTGTTCTCAAA+AGG | 0.466425 | 5.1:-14752153 | MS.gene86558:CDS |
| AACATCTTGTCTGAATCTCT+TGG | 0.478887 | 5.1:+14751390 | None:intergenic |
| GAGAATTTGGTATTCCTCGC+TGG | 0.487057 | 5.1:-14752123 | MS.gene86558:CDS |
| CAGCCATGGCAGTGATAAGA+AGG | 0.499451 | 5.1:-14751480 | MS.gene86558:CDS |
| GCAGGAAGAGGCAAAACATC+AGG | 0.500010 | 5.1:-14751521 | MS.gene86558:intron |
| GATAAGAAGGCAAAAGATGT+TGG | 0.505266 | 5.1:-14751467 | MS.gene86558:CDS |
| AATTGGCATATCAAAGTACT+TGG | 0.526594 | 5.1:+14752201 | None:intergenic |
| AATCTTTGCAACATGGTCAC+TGG | 0.527182 | 5.1:+14752240 | None:intergenic |
| AGAGGCAAAACATCAGGAAA+GGG | 0.535237 | 5.1:-14751515 | MS.gene86558:CDS |
| ATCAGAAGATGCTAATTGCT+AGG | 0.535738 | 5.1:+14751304 | None:intergenic |
| GCGTCAAAGAATCTAAATGT+TGG | 0.549395 | 5.1:-14752172 | MS.gene86558:CDS |
| AAGAGGCAAAACATCAGGAA+AGG | 0.561722 | 5.1:-14751516 | MS.gene86558:CDS |
| AAAAGGAAAACATAGAGAAG+AGG | 0.578123 | 5.1:-14751438 | MS.gene86558:CDS |
| GGAGGATAAAGATGCAGCCA+TGG | 0.600209 | 5.1:-14751494 | MS.gene86558:CDS |
| AGTGATTTGATCTTCCTATG+AGG | 0.604696 | 5.1:+14752100 | None:intergenic |
| GGCAAAACATCAGGAAAGGG+AGG | 0.624378 | 5.1:-14751512 | MS.gene86558:CDS |
| TTAAAGTAATCTTTGCAACA+TGG | 0.639737 | 5.1:+14752233 | None:intergenic |
| ATCTTCCTATGAGGCCAGCG+AGG | 0.669880 | 5.1:+14752109 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTCATAATAATAAATAAAAA+AGG | + | chr5.1:14751541-14751560 | None:intergenic | 10.0% |
| !! | AAAGATTACTTTAACAGATT+TGG | - | chr5.1:14751357-14751376 | MS.gene86558:CDS | 20.0% |
| !! | ATGAAAATTTATAGTATCTG+AGG | - | chr5.1:14751724-14751743 | MS.gene86558:intron | 20.0% |
| !! | TATTTCAACCAAATACTATA+AGG | + | chr5.1:14751699-14751718 | None:intergenic | 20.0% |
| !! | TTATAGACCAAATACTTTAT+AGG | + | chr5.1:14751952-14751971 | None:intergenic | 20.0% |
| !! | TTGTAACTTAAACAAAATCA+CGG | + | chr5.1:14751850-14751869 | None:intergenic | 20.0% |
| ! | ATTGCAAACCTTATAGTATT+TGG | - | chr5.1:14751688-14751707 | MS.gene86558:intron | 25.0% |
| ! | CTAAATATTAGTTCAAGAGT+TGG | + | chr5.1:14751638-14751657 | None:intergenic | 25.0% |
| ! | GTACAGAAAATATTTGTACA+AGG | + | chr5.1:14751767-14751786 | None:intergenic | 25.0% |
| ! | GTTGTCAATTAGTATATGTT+TGG | - | chr5.1:14751791-14751810 | MS.gene86558:intron | 25.0% |
| ! | TAAGAAGTTCACTTAAAGAT+TGG | + | chr5.1:14752021-14752040 | None:intergenic | 25.0% |
| ! | TTAAAGTAATCTTTGCAACA+TGG | + | chr5.1:14751351-14751370 | None:intergenic | 25.0% |
| !! | GTAAATCATAGAAGTTTTCT+TGG | + | chr5.1:14751613-14751632 | None:intergenic | 25.0% |
| !!! | GTTTTTTAGGAAGAAAAAGA+GGG | - | chr5.1:14751315-14751334 | MS.gene86558:CDS | 25.0% |
| AAAAGGAAAACATAGAGAAG+AGG | - | chr5.1:14752143-14752162 | MS.gene86558:CDS | 30.0% | |
| AAAGATGTTGGAAAGTGAAA+AGG | - | chr5.1:14752126-14752145 | MS.gene86558:CDS | 30.0% | |
| AAGTTCACTTAAAGATTGGT+AGG | + | chr5.1:14752017-14752036 | None:intergenic | 30.0% | |
| AGAGTTTGATAAAATCACAG+TGG | - | chr5.1:14751822-14751841 | MS.gene86558:intron | 30.0% | |
| CGTCAAAGAATCTAAATGTT+GGG | - | chr5.1:14751410-14751429 | MS.gene86558:CDS | 30.0% | |
| ! | AATTGGCATATCAAAGTACT+TGG | + | chr5.1:14751383-14751402 | None:intergenic | 30.0% |
| ! | ACTGAAGCCTATAAAGTATT+TGG | - | chr5.1:14751942-14751961 | MS.gene86558:intron | 30.0% |
| AACATCTTGTCTGAATCTCT+TGG | + | chr5.1:14752194-14752213 | None:intergenic | 35.0% | |
| AGTGATTTGATCTTCCTATG+AGG | + | chr5.1:14751484-14751503 | None:intergenic | 35.0% | |
| GATAAGAAGGCAAAAGATGT+TGG | - | chr5.1:14752114-14752133 | MS.gene86558:CDS | 35.0% | |
| GCGTCAAAGAATCTAAATGT+TGG | - | chr5.1:14751409-14751428 | MS.gene86558:CDS | 35.0% | |
| TATAGGCTTCAGTGCATATT+TGG | + | chr5.1:14751935-14751954 | None:intergenic | 35.0% | |
| ! | AAAAGGAAGTGCAGAGAATT+TGG | - | chr5.1:14751445-14751464 | MS.gene86558:CDS | 35.0% |
| ! | TAGCTTTTGTGCCAAAATCA+CGG | - | chr5.1:14751874-14751893 | MS.gene86558:intron | 35.0% |
| ! | TATCTGAGGCTTCTTTAGAT+GGG | - | chr5.1:14751738-14751757 | MS.gene86558:intron | 35.0% |
| !! | GACAAGATGTTTTCAAGAGA+AGG | - | chr5.1:14752203-14752222 | MS.gene86558:CDS | 35.0% |
| AAGAGGCAAAACATCAGGAA+AGG | - | chr5.1:14752065-14752084 | MS.gene86558:intron | 40.0% | |
| AATCTTTGCAACATGGTCAC+TGG | + | chr5.1:14751344-14751363 | None:intergenic | 40.0% | |
| AGAGGCAAAACATCAGGAAA+GGG | - | chr5.1:14752066-14752085 | MS.gene86558:intron | 40.0% | |
| AGCACCTAAACCTAGCAATT+AGG | - | chr5.1:14752267-14752286 | MS.gene86558:CDS | 40.0% | |
| CAGTCTCATTCATGATCTTC+AGG | - | chr5.1:14751507-14751526 | MS.gene86558:CDS | 40.0% | |
| GCTTCAGTGCATATTTGGAT+TGG | + | chr5.1:14751930-14751949 | None:intergenic | 40.0% | |
| TTCTTTGACGCTTCAGCAAT+TGG | + | chr5.1:14751400-14751419 | None:intergenic | 40.0% | |
| TTGGGCTTACTGTTCTCAAA+AGG | - | chr5.1:14751428-14751447 | MS.gene86558:CDS | 40.0% | |
| ! | GTATCTGAGGCTTCTTTAGA+TGG | - | chr5.1:14751737-14751756 | MS.gene86558:intron | 40.0% |
| CATAGAGAAGAGGCTCTTCA+TGG | - | chr5.1:14752153-14752172 | MS.gene86558:CDS | 45.0% | |
| GAGAATTTGGTATTCCTCGC+TGG | - | chr5.1:14751458-14751477 | MS.gene86558:CDS | 45.0% | |
| TTGCCTTCTTATCACTGCCA+TGG | + | chr5.1:14752107-14752126 | None:intergenic | 45.0% | |
| ! | CTTTTGTGCCAAAATCACGG+TGG | - | chr5.1:14751877-14751896 | MS.gene86558:intron | 45.0% |
| !! | TCATAATAATAAATAAAAAA+GGG | + | chr5.1:14751540-14751559 | None:intergenic | 5.0% |
| ACACATGACAACATCGCTGC+AGG | - | chr5.1:14752042-14752061 | MS.gene86558:intron | 50.0% | |
| CAGCCATGGCAGTGATAAGA+AGG | - | chr5.1:14752101-14752120 | MS.gene86558:CDS | 50.0% | |
| GCAGGAAGAGGCAAAACATC+AGG | - | chr5.1:14752060-14752079 | MS.gene86558:intron | 50.0% | |
| GGAGGATAAAGATGCAGCCA+TGG | - | chr5.1:14752087-14752106 | MS.gene86558:CDS | 50.0% | |
| GGCAAAACATCAGGAAAGGG+AGG | - | chr5.1:14752069-14752088 | MS.gene86558:intron | 50.0% | |
| ! | ACGGTGAACCACCGTGATTT+TGG | + | chr5.1:14751888-14751907 | None:intergenic | 50.0% |
| ACGGTGGTTCACCGTGATTC+GGG | - | chr5.1:14751893-14751912 | MS.gene86558:intron | 55.0% | |
| ATCTTCCTATGAGGCCAGCG+AGG | + | chr5.1:14751475-14751494 | None:intergenic | 55.0% | |
| CACGGTGGTTCACCGTGATT+CGG | - | chr5.1:14751892-14751911 | MS.gene86558:intron | 55.0% | |
| CGTTGAGACTGCCCGAATCA+CGG | + | chr5.1:14751907-14751926 | None:intergenic | 55.0% | |
| GACAACATCGCTGCAGGAAG+AGG | - | chr5.1:14752048-14752067 | MS.gene86558:intron | 55.0% | |
| ! | GTATTCCTCGCTGGCCTCAT+AGG | - | chr5.1:14751467-14751486 | MS.gene86558:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 14751315 | 14752288 | 14751315 | ID=MS.gene86558 |
| chr5.1 | mRNA | 14751315 | 14752288 | 14751315 | ID=MS.gene86558.t1;Parent=MS.gene86558 |
| chr5.1 | exon | 14752075 | 14752288 | 14752075 | ID=MS.gene86558.t1.exon1;Parent=MS.gene86558.t1 |
| chr5.1 | CDS | 14752075 | 14752288 | 14752075 | ID=cds.MS.gene86558.t1;Parent=MS.gene86558.t1 |
| chr5.1 | exon | 14751315 | 14751539 | 14751315 | ID=MS.gene86558.t1.exon2;Parent=MS.gene86558.t1 |
| chr5.1 | CDS | 14751315 | 14751539 | 14751315 | ID=cds.MS.gene86558.t1;Parent=MS.gene86558.t1 |
| Gene Sequence |
| Protein sequence |
