AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86795


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86795.t1 MTR_6g032970 90.341 176 4 1 15 190 279 441 5.81e-107 320
MS.gene86795.t1 MTR_7g085500 64.362 188 61 4 7 190 286 471 1.33e-72 231
MS.gene86795.t1 MTR_8g088390 52.809 178 71 7 22 190 222 395 2.13e-44 155
MS.gene86795.t1 MTR_8g088390 52.809 178 71 7 22 190 287 460 2.67e-44 155
MS.gene86795.t1 MTR_4g005110 35.714 182 87 6 14 190 278 434 1.07e-18 83.6
MS.gene86795.t1 MTR_2g032660 41.463 123 61 3 67 189 293 404 1.14e-17 80.5
MS.gene86795.t1 MTR_4g123630 40.136 147 63 4 49 190 245 371 1.56e-17 80.1
MS.gene86795.t1 MTR_2g063460 43.750 112 37 3 85 190 320 411 3.03e-17 79.3
MS.gene86795.t1 MTR_2g063460 43.750 112 37 3 85 190 322 413 3.15e-17 79.3
MS.gene86795.t1 MTR_2g005840 61.818 55 21 0 136 190 236 290 1.36e-16 77.0
MS.gene86795.t1 MTR_2g005840 61.818 55 21 0 136 190 267 321 1.45e-16 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86795.t1 AT3G13460 46.919 211 85 9 2 190 276 481 2.57e-38 139
MS.gene86795.t1 AT3G13460 46.919 211 85 9 2 190 276 481 2.70e-38 137
MS.gene86795.t1 AT3G13460 46.919 211 85 9 2 190 273 478 2.72e-38 139
MS.gene86795.t1 AT3G13460 46.919 211 85 9 2 190 275 480 3.06e-38 139
MS.gene86795.t1 AT1G55500 45.062 162 74 4 37 190 243 397 1.41e-31 119
MS.gene86795.t1 AT1G55500 45.062 162 74 4 37 190 299 453 1.41e-31 120
MS.gene86795.t1 AT1G55500 45.062 162 74 4 37 190 299 453 1.47e-31 120
MS.gene86795.t1 AT1G55500 45.062 162 74 4 37 190 286 440 1.56e-31 120
MS.gene86795.t1 AT1G55500 45.062 162 74 4 37 190 256 410 1.59e-31 119
MS.gene86795.t1 AT1G55500 45.062 162 74 4 37 190 299 453 1.91e-31 119
MS.gene86795.t1 AT3G03950 40.936 171 77 6 28 190 127 281 4.60e-25 100
MS.gene86795.t1 AT3G03950 40.936 171 77 6 28 190 126 280 5.36e-25 100
MS.gene86795.t1 AT3G03950 40.936 171 77 6 28 190 130 284 5.59e-25 100
MS.gene86795.t1 AT5G61020 40.223 179 94 6 17 190 128 298 2.32e-24 99.4
MS.gene86795.t1 AT5G61020 40.223 179 94 6 17 190 130 300 2.53e-24 99.4
MS.gene86795.t1 AT3G13060 35.922 206 102 7 11 190 236 437 7.61e-20 86.7
MS.gene86795.t1 AT3G13060 36.585 205 102 7 11 190 236 437 7.74e-20 86.7
MS.gene86795.t1 AT3G13060 35.922 206 102 7 11 190 226 427 7.80e-20 86.7
MS.gene86795.t1 AT3G13060 35.922 206 102 7 11 190 214 415 8.62e-20 86.7
MS.gene86795.t1 AT3G13060 35.922 206 102 7 11 190 214 415 8.62e-20 86.7
MS.gene86795.t1 AT3G13060 35.922 206 102 7 11 190 236 437 8.82e-20 86.3
MS.gene86795.t1 AT3G17330 59.701 67 25 1 124 190 143 207 1.07e-18 83.2
MS.gene86795.t1 AT1G48110 71.698 53 15 0 138 190 308 360 1.41e-18 83.2
MS.gene86795.t1 AT1G48110 71.698 53 15 0 138 190 308 360 1.41e-18 83.2
MS.gene86795.t1 AT1G09810 35.359 181 87 7 23 190 107 270 1.47e-18 82.8
MS.gene86795.t1 AT1G09810 35.359 181 87 7 23 190 88 251 1.69e-18 82.8
MS.gene86795.t1 AT3G17330 59.701 67 25 1 124 190 245 309 1.74e-18 82.8
MS.gene86795.t1 AT3G17330 59.701 67 25 1 124 190 245 309 1.74e-18 82.8
MS.gene86795.t1 AT1G09810 67.273 55 18 0 136 190 174 228 2.11e-18 82.4
MS.gene86795.t1 AT1G27960 40.351 114 43 2 77 190 281 369 6.00e-18 80.9
MS.gene86795.t1 AT1G27960 40.351 114 43 2 77 190 207 295 8.58e-18 80.9
MS.gene86795.t1 AT1G79270 67.241 58 19 0 133 190 288 345 8.67e-18 80.9
MS.gene86795.t1 AT1G79270 67.241 58 19 0 133 190 288 345 8.67e-18 80.9
MS.gene86795.t1 AT1G79270 67.241 58 19 0 133 190 288 345 8.67e-18 80.9
MS.gene86795.t1 AT1G79270 67.241 58 19 0 133 190 303 360 8.88e-18 80.9
MS.gene86795.t1 AT1G27960 40.351 114 43 2 77 190 281 369 1.10e-17 80.5
MS.gene86795.t1 AT5G58190 42.000 100 48 3 91 190 275 364 3.44e-16 76.3
MS.gene86795.t1 AT5G58190 42.000 100 48 3 91 190 274 363 3.47e-16 76.3

Find 54 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCTCAACTATTGTTTCT+AGG 0.202952 3.4:+24081863 MS.gene86795:CDS
CTCTGTATGTGTTTCCATAT+TGG 0.246769 3.4:-24081972 None:intergenic
TCTGGACTGGGACCGTATTC+TGG 0.259091 3.4:+24081910 MS.gene86795:CDS
TCTAACTATGGATCTACTTA+TGG 0.286333 3.4:+24082000 MS.gene86795:CDS
TCTAGCTTCTTGTTGCCATT+AGG 0.298283 3.4:-24082378 None:intergenic
CTAGCTTCTTGTTGCCATTA+GGG 0.298390 3.4:-24082377 None:intergenic
GAAGCTAGATGCGGCATATC+AGG 0.346860 3.4:+24082392 MS.gene86795:CDS
CCGTATTCTGGATATCCTAA+TGG 0.351387 3.4:+24081922 MS.gene86795:CDS
GTTATCATAGGAACCTGTTC+TGG 0.374797 3.4:-24082025 None:intergenic
TTATCATCATCACTCTTGAT+AGG 0.378740 3.4:-24082210 None:intergenic
AACACGATGTACAGCCAATA+TGG 0.389273 3.4:+24081958 MS.gene86795:CDS
CCGGTGTATTGTTGCCTGTC+AGG 0.398793 3.4:-24082249 None:intergenic
TTGCTTGGGTACATCCCATT+AGG 0.407428 3.4:-24081937 None:intergenic
AACTTGCATGGCTATTCATC+TGG 0.421844 3.4:+24081892 MS.gene86795:CDS
TACAGAGGCAGGTCTAACTA+TGG 0.441046 3.4:+24081988 MS.gene86795:CDS
CGTATTCTGGATATCCTAAT+GGG 0.442464 3.4:+24081923 MS.gene86795:CDS
CATGGCTATTCATCTGGACT+GGG 0.442552 3.4:+24081898 MS.gene86795:CDS
TATTGTTGCCTGTCAGGAAC+TGG 0.454396 3.4:-24082243 None:intergenic
TGGCTGTACATCGTGTTGCT+TGG 0.469761 3.4:-24081952 None:intergenic
AAAAGAAATGTGGATGGCTT+TGG 0.470712 3.4:+24082090 MS.gene86795:CDS
GGCTGTACATCGTGTTGCTT+GGG 0.474945 3.4:-24081951 None:intergenic
GCATGGCTATTCATCTGGAC+TGG 0.477233 3.4:+24081897 MS.gene86795:CDS
GGATCTATACTTGTTATCAT+AGG 0.478586 3.4:-24082037 None:intergenic
TCTACTTATGGATCCAGAAC+AGG 0.481498 3.4:+24082012 MS.gene86795:CDS
CACATTATTACTCAAGGGTC+AGG 0.490934 3.4:+24082182 MS.gene86795:CDS
GTGTGGACAAGTACCCCTAA+TGG 0.497541 3.4:+24082363 MS.gene86795:CDS
CCATTAGGATATCCAGAATA+CGG 0.504485 3.4:-24081922 None:intergenic
ACCTGTCACATTATTACTCA+AGG 0.514931 3.4:+24082176 MS.gene86795:CDS
AAGTATAGATCCACACGCTA+TGG 0.515371 3.4:+24082048 MS.gene86795:CDS
CCCTTGAGTAATAATGTGAC+AGG 0.522853 3.4:-24082177 None:intergenic
TAGCTTCTTGTTGCCATTAG+GGG 0.524738 3.4:-24082376 None:intergenic
AAGTGCTTCCAGTTCCTGAC+AGG 0.533740 3.4:+24082235 MS.gene86795:CDS
CATCATCACTCTTGATAGGA+AGG 0.537515 3.4:-24082206 None:intergenic
TCTAGGTTGCAGAACTTGCA+TGG 0.539046 3.4:+24081880 MS.gene86795:CDS
ATGGAAACACATACAGAGGC+AGG 0.547380 3.4:+24081977 MS.gene86795:CDS
CTGACAGGCAACAATACACC+GGG 0.567636 3.4:+24082250 MS.gene86795:CDS
ATTCTCAGATAAATCTTCCC+CGG 0.595705 3.4:-24082268 None:intergenic
TGGCTTTGGTGAGCTGAACA+AGG 0.609686 3.4:+24082104 MS.gene86795:CDS
TGTCATCAAATCTTATAGCG+AGG 0.611587 3.4:+24082311 MS.gene86795:CDS
AGAGTATGAAATATAATGTG+TGG 0.612407 3.4:+24082346 MS.gene86795:CDS
TGACCATGTCAAAAGAAATG+TGG 0.616421 3.4:+24082080 MS.gene86795:CDS
TCAGAACTATTGGAACCTCT+CGG 0.617068 3.4:-24082129 None:intergenic
AGATCCACACGCTATGGACG+TGG 0.617909 3.4:+24082054 MS.gene86795:CDS
TGCGGCATATCAGGAAGCCT+AGG 0.627415 3.4:+24082401 MS.gene86795:CDS
CAACCTAGAAACAATAGTTG+AGG 0.643536 3.4:-24081866 None:intergenic
AAAACCACGTCCATAGCGTG+TGG 0.643915 3.4:-24082058 None:intergenic
CCTGACAGGCAACAATACAC+CGG 0.643940 3.4:+24082249 MS.gene86795:CDS
CCTGTCACATTATTACTCAA+GGG 0.647177 3.4:+24082177 MS.gene86795:CDS
GGCTTTGGTGAGCTGAACAA+GGG 0.653383 3.4:+24082105 MS.gene86795:CDS
CATGTCAAAAGAAATGTGGA+TGG 0.682176 3.4:+24082084 MS.gene86795:CDS
CAATATGGAAACACATACAG+AGG 0.684546 3.4:+24081973 MS.gene86795:CDS
GAGCTGAACAAGGGACCGAG+AGG 0.693881 3.4:+24082114 MS.gene86795:CDS
TGGCAACAAGAAGCTAGATG+CGG 0.702216 3.4:+24082383 MS.gene86795:CDS
TGACAGGCAACAATACACCG+GGG 0.819631 3.4:+24082251 MS.gene86795:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTATCATCAGAACTAT+TGG - chr3.4:24082142-24082161 None:intergenic 20.0%
! AGAGTATGAAATATAATGTG+TGG + chr3.4:24082346-24082365 MS.gene86795:CDS 25.0%
! GATAAAAATGACAAAAGTCT+TGG + chr3.4:24082153-24082172 MS.gene86795:CDS 25.0%
TCTAACTATGGATCTACTTA+TGG + chr3.4:24082000-24082019 MS.gene86795:CDS 30.0%
TTATCATCATCACTCTTGAT+AGG - chr3.4:24082213-24082232 None:intergenic 30.0%
!! GGATCTATACTTGTTATCAT+AGG - chr3.4:24082040-24082059 None:intergenic 30.0%
AAAAGAAATGTGGATGGCTT+TGG + chr3.4:24082090-24082109 MS.gene86795:CDS 35.0%
ACCTGTCACATTATTACTCA+AGG + chr3.4:24082176-24082195 MS.gene86795:CDS 35.0%
ATTCTCAGATAAATCTTCCC+CGG - chr3.4:24082271-24082290 None:intergenic 35.0%
CAACCTAGAAACAATAGTTG+AGG - chr3.4:24081869-24081888 None:intergenic 35.0%
CAATATGGAAACACATACAG+AGG + chr3.4:24081973-24081992 MS.gene86795:CDS 35.0%
CATGTCAAAAGAAATGTGGA+TGG + chr3.4:24082084-24082103 MS.gene86795:CDS 35.0%
CCATTAGGATATCCAGAATA+CGG - chr3.4:24081925-24081944 None:intergenic 35.0%
CCTGTCACATTATTACTCAA+GGG + chr3.4:24082177-24082196 MS.gene86795:CDS 35.0%
CGTATTCTGGATATCCTAAT+GGG + chr3.4:24081923-24081942 MS.gene86795:CDS 35.0%
CTCTGTATGTGTTTCCATAT+TGG - chr3.4:24081975-24081994 None:intergenic 35.0%
TGACCATGTCAAAAGAAATG+TGG + chr3.4:24082080-24082099 MS.gene86795:CDS 35.0%
TGTCATCAAATCTTATAGCG+AGG + chr3.4:24082311-24082330 MS.gene86795:CDS 35.0%
! CATCCACATTTCTTTTGACA+TGG - chr3.4:24082086-24082105 None:intergenic 35.0%
! TCACCTCAACTATTGTTTCT+AGG + chr3.4:24081863-24081882 MS.gene86795:CDS 35.0%
AACACGATGTACAGCCAATA+TGG + chr3.4:24081958-24081977 MS.gene86795:CDS 40.0%
AACTTGCATGGCTATTCATC+TGG + chr3.4:24081892-24081911 MS.gene86795:CDS 40.0%
AAGTATAGATCCACACGCTA+TGG + chr3.4:24082048-24082067 MS.gene86795:CDS 40.0%
CACATTATTACTCAAGGGTC+AGG + chr3.4:24082182-24082201 MS.gene86795:CDS 40.0%
CATCATCACTCTTGATAGGA+AGG - chr3.4:24082209-24082228 None:intergenic 40.0%
CCCTTGAGTAATAATGTGAC+AGG - chr3.4:24082180-24082199 None:intergenic 40.0%
CCGTATTCTGGATATCCTAA+TGG + chr3.4:24081922-24081941 MS.gene86795:CDS 40.0%
CTAGCTTCTTGTTGCCATTA+GGG - chr3.4:24082380-24082399 None:intergenic 40.0%
GTTATCATAGGAACCTGTTC+TGG - chr3.4:24082028-24082047 None:intergenic 40.0%
TAGCTTCTTGTTGCCATTAG+GGG - chr3.4:24082379-24082398 None:intergenic 40.0%
TCAGAACTATTGGAACCTCT+CGG - chr3.4:24082132-24082151 None:intergenic 40.0%
TCTACTTATGGATCCAGAAC+AGG + chr3.4:24082012-24082031 MS.gene86795:CDS 40.0%
! TCTAGCTTCTTGTTGCCATT+AGG - chr3.4:24082381-24082400 None:intergenic 40.0%
ATGGAAACACATACAGAGGC+AGG + chr3.4:24081977-24081996 MS.gene86795:CDS 45.0%
CATGGCTATTCATCTGGACT+GGG + chr3.4:24081898-24081917 MS.gene86795:CDS 45.0%
TACAGAGGCAGGTCTAACTA+TGG + chr3.4:24081988-24082007 MS.gene86795:CDS 45.0%
TATTGTTGCCTGTCAGGAAC+TGG - chr3.4:24082246-24082265 None:intergenic 45.0%
TCTAGGTTGCAGAACTTGCA+TGG + chr3.4:24081880-24081899 MS.gene86795:CDS 45.0%
TGGCAACAAGAAGCTAGATG+CGG + chr3.4:24082383-24082402 MS.gene86795:CDS 45.0%
TTGCTTGGGTACATCCCATT+AGG - chr3.4:24081940-24081959 None:intergenic 45.0%
AAAACCACGTCCATAGCGTG+TGG - chr3.4:24082061-24082080 None:intergenic 50.0%
AAGTGCTTCCAGTTCCTGAC+AGG + chr3.4:24082235-24082254 MS.gene86795:CDS 50.0%
CCTGACAGGCAACAATACAC+CGG + chr3.4:24082249-24082268 MS.gene86795:CDS 50.0%
CTGACAGGCAACAATACACC+GGG + chr3.4:24082250-24082269 MS.gene86795:CDS 50.0%
GAAGCTAGATGCGGCATATC+AGG + chr3.4:24082392-24082411 MS.gene86795:CDS 50.0%
GCATGGCTATTCATCTGGAC+TGG + chr3.4:24081897-24081916 MS.gene86795:CDS 50.0%
GTGTGGACAAGTACCCCTAA+TGG + chr3.4:24082363-24082382 MS.gene86795:CDS 50.0%
TGACAGGCAACAATACACCG+GGG + chr3.4:24082251-24082270 MS.gene86795:CDS 50.0%
! GGCTTTGGTGAGCTGAACAA+GGG + chr3.4:24082105-24082124 MS.gene86795:CDS 50.0%
! TGGCTGTACATCGTGTTGCT+TGG - chr3.4:24081955-24081974 None:intergenic 50.0%
!! GGCTGTACATCGTGTTGCTT+GGG - chr3.4:24081954-24081973 None:intergenic 50.0%
!! TGGCTTTGGTGAGCTGAACA+AGG + chr3.4:24082104-24082123 MS.gene86795:CDS 50.0%
AGATCCACACGCTATGGACG+TGG + chr3.4:24082054-24082073 MS.gene86795:CDS 55.0%
CCGGTGTATTGTTGCCTGTC+AGG - chr3.4:24082252-24082271 None:intergenic 55.0%
! TCTGGACTGGGACCGTATTC+TGG + chr3.4:24081910-24081929 MS.gene86795:CDS 55.0%
GAGCTGAACAAGGGACCGAG+AGG + chr3.4:24082114-24082133 MS.gene86795:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 24081850 24082422 24081850 ID=MS.gene86795
chr3.4 mRNA 24081850 24082422 24081850 ID=MS.gene86795.t1;Parent=MS.gene86795
chr3.4 exon 24081850 24082422 24081850 ID=MS.gene86795.t1.exon1;Parent=MS.gene86795.t1
chr3.4 CDS 24081850 24082422 24081850 ID=cds.MS.gene86795.t1;Parent=MS.gene86795.t1
Gene Sequence

>MS.gene86795

ATGTATTCAAAATTCACCTCAACTATTGTTTCTAGGTTGCAGAACTTGCATGGCTATTCATCTGGACTGGGACCGTATTCTGGATATCCTAATGGGATGTACCCAAGCAACACGATGTACAGCCAATATGGAAACACATACAGAGGCAGGTCTAACTATGGATCTACTTATGGATCCAGAACAGGTTCCTATGATAACAAGTATAGATCCACACGCTATGGACGTGGTTTTGACCATGTCAAAAGAAATGTGGATGGCTTTGGTGAGCTGAACAAGGGACCGAGAGGTTCCAATAGTTCTGATGATAAAAATGACAAAAGTCTTGGACCTGTCACATTATTACTCAAGGGTCAGGACCTTCCTATCAAGAGTGATGATGATAAAGAAGTGCTTCCAGTTCCTGACAGGCAACAATACACCGGGGAAGATTTATCTGAGAATTATTCTGATGCAAAGTTTTTTGTCATCAAATCTTATAGCGAGGATGATATTCACAAGAGTATGAAATATAATGTGTGGACAAGTACCCCTAATGGCAACAAGAAGCTAGATGCGGCATATCAGGAAGCCTAG

Protein sequence

>MS.gene86795.t1

MYSKFTSTIVSRLQNLHGYSSGLGPYSGYPNGMYPSNTMYSQYGNTYRGRSNYGSTYGSRTGSYDNKYRSTRYGRGFDHVKRNVDGFGELNKGPRGSNSSDDKNDKSLGPVTLLLKGQDLPIKSDDDKEVLPVPDRQQYTGEDLSENYSDAKFFVIKSYSEDDIHKSMKYNVWTSTPNGNKKLDAAYQEA