Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86798.t1 | XP_013442640.1 | 71.7 | 237 | 31 | 2 | 1 | 202 | 1 | 236 | 5.20E-85 | 323.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86798.t1 | Q9FKK2 | 37.1 | 167 | 67 | 3 | 5 | 133 | 3 | 169 | 7.9e-18 | 92.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86798.t1 | A0A072TSY1 | 71.7 | 237 | 31 | 2 | 1 | 202 | 1 | 236 | 3.7e-85 | 323.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene86798 | MS.gene22094 | PPI |
| MS.gene86798 | MS.gene068836 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86798.t1 | MTR_0121s0080 | 71.730 | 237 | 31 | 2 | 1 | 202 | 1 | 236 | 1.45e-115 | 328 |
| MS.gene86798.t1 | MTR_7g062350 | 84.831 | 178 | 26 | 1 | 25 | 202 | 72 | 248 | 1.96e-106 | 306 |
| MS.gene86798.t1 | MTR_8g043650 | 62.025 | 237 | 55 | 1 | 1 | 202 | 1 | 237 | 9.82e-99 | 286 |
| MS.gene86798.t1 | MTR_2g030740 | 61.603 | 237 | 56 | 1 | 1 | 202 | 1 | 237 | 1.46e-87 | 258 |
| MS.gene86798.t1 | MTR_1g105910 | 59.494 | 237 | 61 | 1 | 1 | 202 | 1 | 237 | 7.69e-85 | 251 |
| MS.gene86798.t1 | MTR_1g105920 | 61.842 | 228 | 52 | 1 | 1 | 193 | 1 | 228 | 2.15e-84 | 249 |
| MS.gene86798.t1 | MTR_1g012570 | 61.181 | 237 | 57 | 1 | 1 | 202 | 1 | 237 | 9.70e-84 | 248 |
| MS.gene86798.t1 | MTR_1g108510 | 57.021 | 235 | 48 | 4 | 1 | 190 | 1 | 227 | 1.87e-79 | 237 |
| MS.gene86798.t1 | MTR_2g093190 | 57.872 | 235 | 46 | 4 | 1 | 190 | 1 | 227 | 1.91e-79 | 237 |
| MS.gene86798.t1 | MTR_3g052920 | 56.621 | 219 | 60 | 1 | 1 | 184 | 1 | 219 | 1.38e-78 | 235 |
| MS.gene86798.t1 | MTR_3g030770 | 57.021 | 235 | 48 | 4 | 1 | 190 | 1 | 227 | 2.63e-78 | 234 |
| MS.gene86798.t1 | MTR_1g105905 | 62.360 | 178 | 67 | 0 | 25 | 202 | 43 | 220 | 1.69e-76 | 229 |
| MS.gene86798.t1 | MTR_1g108580 | 55.932 | 236 | 49 | 4 | 1 | 190 | 1 | 227 | 3.01e-76 | 229 |
| MS.gene86798.t1 | MTR_1g075600 | 53.165 | 237 | 76 | 1 | 1 | 202 | 1 | 237 | 6.73e-73 | 220 |
| MS.gene86798.t1 | MTR_2g085280 | 49.576 | 236 | 84 | 1 | 1 | 201 | 1 | 236 | 3.03e-72 | 219 |
| MS.gene86798.t1 | MTR_2g085250 | 49.576 | 236 | 84 | 1 | 1 | 201 | 1 | 236 | 1.12e-71 | 217 |
| MS.gene86798.t1 | MTR_5g055100 | 49.367 | 237 | 85 | 1 | 1 | 202 | 1 | 237 | 2.69e-71 | 216 |
| MS.gene86798.t1 | MTR_1g077360 | 51.111 | 225 | 75 | 1 | 1 | 190 | 1 | 225 | 5.06e-71 | 216 |
| MS.gene86798.t1 | MTR_5g053390 | 50.667 | 225 | 76 | 1 | 1 | 190 | 1 | 225 | 1.08e-67 | 207 |
| MS.gene86798.t1 | MTR_5g045560 | 49.333 | 225 | 79 | 1 | 1 | 190 | 1 | 225 | 1.20e-65 | 202 |
| MS.gene86798.t1 | MTR_1g106070 | 56.906 | 181 | 43 | 1 | 1 | 146 | 1 | 181 | 3.61e-63 | 194 |
| MS.gene86798.t1 | MTR_3g052870 | 53.947 | 228 | 51 | 3 | 1 | 193 | 1 | 209 | 1.48e-62 | 193 |
| MS.gene86798.t1 | MTR_3g030780 | 64.394 | 132 | 12 | 1 | 1 | 97 | 1 | 132 | 3.23e-48 | 154 |
| MS.gene86798.t1 | MTR_0121s0100 | 53.030 | 132 | 27 | 1 | 1 | 97 | 1 | 132 | 3.83e-33 | 115 |
| MS.gene86798.t1 | MTR_1g075570 | 46.951 | 164 | 66 | 2 | 18 | 160 | 1 | 164 | 1.20e-32 | 115 |
| MS.gene86798.t1 | MTR_3g080940 | 38.938 | 113 | 69 | 0 | 25 | 137 | 60 | 172 | 8.31e-23 | 91.7 |
| MS.gene86798.t1 | MTR_3g067915 | 47.917 | 96 | 45 | 1 | 1 | 91 | 1 | 96 | 2.69e-22 | 87.0 |
| MS.gene86798.t1 | MTR_3g067910 | 55.556 | 63 | 28 | 0 | 25 | 87 | 41 | 103 | 2.81e-17 | 74.3 |
| MS.gene86798.t1 | MTR_1g108500 | 61.017 | 59 | 2 | 1 | 25 | 83 | 148 | 185 | 6.98e-13 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86798.t1 | AT5G60440 | 36.905 | 168 | 68 | 3 | 5 | 134 | 3 | 170 | 7.83e-23 | 93.6 |
Find 46 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAATTGGCTTCAACAATA+TGG | 0.202381 | 3.4:-24105357 | MS.gene86798:CDS |
| TGCTGATAGGATTGCAATTC+AGG | 0.214281 | 3.4:-24105516 | MS.gene86798:CDS |
| TGAATTATTCAAGAAGATTT+TGG | 0.235888 | 3.4:-24105567 | MS.gene86798:CDS |
| ATCTCAGTTTGTTGAGGATT+TGG | 0.255732 | 3.4:+24105422 | None:intergenic |
| ATGTTGCAACCTCATTTAAT+TGG | 0.256996 | 3.4:-24105371 | MS.gene86798:CDS |
| AAATCATTACCAACAGAAAA+TGG | 0.265409 | 3.4:+24105473 | None:intergenic |
| GTCATAGATCGCTGTCTCTC+TGG | 0.269734 | 3.4:-24105797 | MS.gene86798:CDS |
| TGGTGGGCTTGTCCGATTAA+TGG | 0.300675 | 3.4:-24105608 | MS.gene86798:CDS |
| TCCGATTAATGGGATGAATA+AGG | 0.334528 | 3.4:-24105597 | MS.gene86798:CDS |
| AGGCGTTCAAGGCTCAATTT+TGG | 0.334747 | 3.4:-24105628 | MS.gene86798:CDS |
| TAGAACTTCAATAGAATCTT+GGG | 0.354932 | 3.4:+24105312 | None:intergenic |
| GTGAGCCTCAATAAATTCTA+TGG | 0.360010 | 3.4:+24105751 | None:intergenic |
| GCCTTATTCATCCCATTAAT+CGG | 0.363704 | 3.4:+24105596 | None:intergenic |
| GGTGGGCTTGTCCGATTAAT+GGG | 0.369852 | 3.4:-24105607 | MS.gene86798:CDS |
| GTTGAGGATTTGGCTGATAA+TGG | 0.382243 | 3.4:+24105432 | None:intergenic |
| TTAATTGGCTTCAACAATAT+GGG | 0.383325 | 3.4:-24105356 | MS.gene86798:CDS |
| TCATAGATCGCTGTCTCTCT+GGG | 0.392569 | 3.4:-24105796 | MS.gene86798:CDS |
| CTAGAACTTCAATAGAATCT+TGG | 0.400586 | 3.4:+24105311 | None:intergenic |
| TTGTTGCTCATCATGCTGAT+AGG | 0.400809 | 3.4:-24105529 | MS.gene86798:CDS |
| ATTAAATGAGGTTGCAACAT+AGG | 0.428043 | 3.4:+24105374 | None:intergenic |
| TCTATGACCGTATCAACATT+TGG | 0.448129 | 3.4:+24105812 | None:intergenic |
| AACAATATGGGAGGAGGAGG+AGG | 0.453551 | 3.4:-24105344 | MS.gene86798:CDS |
| ATAATGGAGAGGGATGTTAG+AGG | 0.477682 | 3.4:+24105448 | None:intergenic |
| AGCATTGAGCTCACACACTT+TGG | 0.483043 | 3.4:+24105721 | None:intergenic |
| TTAAATGAGGTTGCAACATA+GGG | 0.493554 | 3.4:+24105375 | None:intergenic |
| ACCTTTCCTTCCGCTTGACA+TGG | 0.495891 | 3.4:+24105989 | None:intergenic |
| CGGTCATCCAAATGTTGATA+CGG | 0.514501 | 3.4:-24105819 | MS.gene86798:CDS |
| GCTGATAGGATTGCAATTCA+GGG | 0.533534 | 3.4:-24105515 | MS.gene86798:CDS |
| GCTCACACACTTTGGCGCTA+CGG | 0.546232 | 3.4:+24105729 | None:intergenic |
| TTCAACAATATGGGAGGAGG+AGG | 0.550029 | 3.4:-24105347 | MS.gene86798:CDS |
| TTGTTGAAGCCAATTAAATG+AGG | 0.551491 | 3.4:+24105362 | None:intergenic |
| GAAAACATCTCAGTTTGTTG+AGG | 0.554882 | 3.4:+24105416 | None:intergenic |
| GGCTTCAACAATATGGGAGG+AGG | 0.561767 | 3.4:-24105350 | MS.gene86798:CDS |
| TAGCACCATAGAATTTATTG+AGG | 0.563308 | 3.4:-24105756 | MS.gene86798:CDS |
| TCAAGCGGAAGGAAAGGTCG+TGG | 0.582976 | 3.4:-24105984 | MS.gene86798:CDS |
| ACCATGTCAAGCGGAAGGAA+AGG | 0.588345 | 3.4:-24105990 | MS.gene86798:CDS |
| CAAGCGGAAGGAAAGGTCGT+GGG | 0.604849 | 3.4:-24105983 | MS.gene86798:CDS |
| ATTGGCTTCAACAATATGGG+AGG | 0.605743 | 3.4:-24105353 | MS.gene86798:CDS |
| AGAACTTCAATAGAATCTTG+GGG | 0.608200 | 3.4:+24105313 | None:intergenic |
| TGTACTTCAACCATGTCAAG+CGG | 0.628063 | 3.4:-24105999 | None:intergenic |
| CTTCAACCATGTCAAGCGGA+AGG | 0.628927 | 3.4:-24105995 | None:intergenic |
| GGATTTGGCTGATAATGGAG+AGG | 0.633381 | 3.4:+24105437 | None:intergenic |
| AATCAACAACACACTAGACG+AGG | 0.684390 | 3.4:-24105687 | MS.gene86798:CDS |
| GATTTGGCTGATAATGGAGA+GGG | 0.685158 | 3.4:+24105438 | None:intergenic |
| GAACTTCAATAGAATCTTGG+GGG | 0.691013 | 3.4:+24105314 | None:intergenic |
| CATAGATCGCTGTCTCTCTG+GGG | 0.696612 | 3.4:-24105795 | MS.gene86798:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAATTATTCAAGAAGATTT+TGG | - | chr3.4:24105739-24105758 | MS.gene86798:CDS | 20.0% |
| !!! | TTTTTTTCATCTCAATCTTT+TGG | + | chr3.4:24105351-24105370 | None:intergenic | 20.0% |
| ! | AAATCATTACCAACAGAAAA+TGG | + | chr3.4:24105836-24105855 | None:intergenic | 25.0% |
| ! | ATAGGGTTATGAAAAATTTG+TGG | + | chr3.4:24105917-24105936 | None:intergenic | 25.0% |
| !! | AAAGAAAATACCTTTTCACT+AGG | + | chr3.4:24105467-24105486 | None:intergenic | 25.0% |
| !! | ATTATTCAAGAAGATTTTGG+TGG | - | chr3.4:24105742-24105761 | MS.gene86798:CDS | 25.0% |
| !! | TTAATTGGCTTCAACAATAT+GGG | - | chr3.4:24105950-24105969 | MS.gene86798:CDS | 25.0% |
| !! | TTTAATTGGCTTCAACAATA+TGG | - | chr3.4:24105949-24105968 | MS.gene86798:CDS | 25.0% |
| !!! | AGTGAAAAGGTATTTTCTTT+CGG | - | chr3.4:24105467-24105486 | MS.gene86798:CDS | 25.0% |
| ATGTTGCAACCTCATTTAAT+TGG | - | chr3.4:24105935-24105954 | MS.gene86798:CDS | 30.0% | |
| ATTAAATGAGGTTGCAACAT+AGG | + | chr3.4:24105935-24105954 | None:intergenic | 30.0% | |
| TAGCACCATAGAATTTATTG+AGG | - | chr3.4:24105550-24105569 | MS.gene86798:CDS | 30.0% | |
| TTAAATGAGGTTGCAACATA+GGG | + | chr3.4:24105934-24105953 | None:intergenic | 30.0% | |
| TTGTTGAAGCCAATTAAATG+AGG | + | chr3.4:24105947-24105966 | None:intergenic | 30.0% | |
| ! | CAAACTTTTCCATTTTCTGT+TGG | - | chr3.4:24105824-24105843 | MS.gene86798:CDS | 30.0% |
| GTGAGCCTCAATAAATTCTA+TGG | + | chr3.4:24105558-24105577 | None:intergenic | 35.0% | |
| TCCGATTAATGGGATGAATA+AGG | - | chr3.4:24105709-24105728 | MS.gene86798:CDS | 35.0% | |
| TCTATGACCGTATCAACATT+TGG | + | chr3.4:24105497-24105516 | None:intergenic | 35.0% | |
| TGTATTCTCACCTAGTGAAA+AGG | - | chr3.4:24105454-24105473 | MS.gene86798:CDS | 35.0% | |
| ! | GCCTTATTCATCCCATTAAT+CGG | + | chr3.4:24105713-24105732 | None:intergenic | 35.0% |
| !! | ATCTCAGTTTGTTGAGGATT+TGG | + | chr3.4:24105887-24105906 | None:intergenic | 35.0% |
| !! | GAAAACATCTCAGTTTGTTG+AGG | + | chr3.4:24105893-24105912 | None:intergenic | 35.0% |
| !!! | TCTATGGTGCTATTGTTTTG+CGG | + | chr3.4:24105542-24105561 | None:intergenic | 35.0% |
| AATCAACAACACACTAGACG+AGG | - | chr3.4:24105619-24105638 | MS.gene86798:CDS | 40.0% | |
| CGGTCATCCAAATGTTGATA+CGG | - | chr3.4:24105487-24105506 | MS.gene86798:CDS | 40.0% | |
| GCTGATAGGATTGCAATTCA+GGG | - | chr3.4:24105791-24105810 | MS.gene86798:CDS | 40.0% | |
| GTTGAGGATTTGGCTGATAA+TGG | + | chr3.4:24105877-24105896 | None:intergenic | 40.0% | |
| TGCTGATAGGATTGCAATTC+AGG | - | chr3.4:24105790-24105809 | MS.gene86798:CDS | 40.0% | |
| TTGTTGCTCATCATGCTGAT+AGG | - | chr3.4:24105777-24105796 | MS.gene86798:CDS | 40.0% | |
| ! | ATAATGGAGAGGGATGTTAG+AGG | + | chr3.4:24105861-24105880 | None:intergenic | 40.0% |
| ! | ATTGGCTTCAACAATATGGG+AGG | - | chr3.4:24105953-24105972 | MS.gene86798:CDS | 40.0% |
| !! | GATTTGGCTGATAATGGAGA+GGG | + | chr3.4:24105871-24105890 | None:intergenic | 40.0% |
| !! | GTTCAAGGCTCAATTTTGGT+GGG | - | chr3.4:24105682-24105701 | MS.gene86798:CDS | 40.0% |
| !! | TGAGTTGAGTCTTTTGTGCA+AGG | - | chr3.4:24105658-24105677 | MS.gene86798:CDS | 40.0% |
| CTAGACGAGGAGAAAAAGCA+TGG | - | chr3.4:24105632-24105651 | MS.gene86798:CDS | 45.0% | |
| TCATAGATCGCTGTCTCTCT+GGG | - | chr3.4:24105510-24105529 | MS.gene86798:CDS | 45.0% | |
| TTCAACAATATGGGAGGAGG+AGG | - | chr3.4:24105959-24105978 | MS.gene86798:CDS | 45.0% | |
| ! | AGCATTGAGCTCACACACTT+TGG | + | chr3.4:24105588-24105607 | None:intergenic | 45.0% |
| ! | AGGCGTTCAAGGCTCAATTT+TGG | - | chr3.4:24105678-24105697 | MS.gene86798:CDS | 45.0% |
| ! | CGTTCAAGGCTCAATTTTGG+TGG | - | chr3.4:24105681-24105700 | MS.gene86798:CDS | 45.0% |
| !! | ACTTTCTCAAAGCGTCGTAG+CGG | - | chr3.4:24105397-24105416 | MS.gene86798:CDS | 45.0% |
| !! | GACGCTTTGAGAAAGTCACT+TGG | + | chr3.4:24105393-24105412 | None:intergenic | 45.0% |
| !! | GCTTTGAGAAAGTCACTTGG+AGG | + | chr3.4:24105390-24105409 | None:intergenic | 45.0% |
| !! | GGATTTGGCTGATAATGGAG+AGG | + | chr3.4:24105872-24105891 | None:intergenic | 45.0% |
| !! | TCTTTTGTGCAAGGCGTTCA+AGG | - | chr3.4:24105667-24105686 | MS.gene86798:CDS | 45.0% |
| !!! | ATGGTGCTATTGTTTTGCGG+TGG | + | chr3.4:24105539-24105558 | None:intergenic | 45.0% |
| !!! | TGGTGCTATTGTTTTGCGGT+GGG | + | chr3.4:24105538-24105557 | None:intergenic | 45.0% |
| AACAATATGGGAGGAGGAGG+AGG | - | chr3.4:24105962-24105981 | MS.gene86798:CDS | 50.0% | |
| CATAGATCGCTGTCTCTCTG+GGG | - | chr3.4:24105511-24105530 | MS.gene86798:CDS | 50.0% | |
| GGCTTCAACAATATGGGAGG+AGG | - | chr3.4:24105956-24105975 | MS.gene86798:CDS | 50.0% | |
| GTCATAGATCGCTGTCTCTC+TGG | - | chr3.4:24105509-24105528 | MS.gene86798:CDS | 50.0% | |
| ! | GGTGGGCTTGTCCGATTAAT+GGG | - | chr3.4:24105699-24105718 | MS.gene86798:CDS | 50.0% |
| !! | CTTTCTCAAAGCGTCGTAGC+GGG | - | chr3.4:24105398-24105417 | MS.gene86798:CDS | 50.0% |
| !! | TGGTGGGCTTGTCCGATTAA+TGG | - | chr3.4:24105698-24105717 | MS.gene86798:CDS | 50.0% |
| ATGGGAGGAGGAGGAGGATA+TGG | - | chr3.4:24105968-24105987 | MS.gene86798:CDS | 55.0% | |
| CAAGCGGAAGGAAAGGTCGT+GGG | - | chr3.4:24105323-24105342 | MS.gene86798:CDS | 55.0% | |
| GCTCACACACTTTGGCGCTA+CGG | + | chr3.4:24105580-24105599 | None:intergenic | 55.0% | |
| TCAAGCGGAAGGAAAGGTCG+TGG | - | chr3.4:24105322-24105341 | MS.gene86798:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 24105319 | 24106009 | 24105319 | ID=MS.gene86798 |
| chr3.4 | mRNA | 24105319 | 24106009 | 24105319 | ID=MS.gene86798.t1;Parent=MS.gene86798 |
| chr3.4 | exon | 24105935 | 24106009 | 24105935 | ID=MS.gene86798.t1.exon1;Parent=MS.gene86798.t1 |
| chr3.4 | CDS | 24105935 | 24106009 | 24105935 | ID=cds.MS.gene86798.t1;Parent=MS.gene86798.t1 |
| chr3.4 | exon | 24105319 | 24105852 | 24105319 | ID=MS.gene86798.t1.exon2;Parent=MS.gene86798.t1 |
| chr3.4 | CDS | 24105319 | 24105852 | 24105319 | ID=cds.MS.gene86798.t1;Parent=MS.gene86798.t1 |
| Gene Sequence |
| Protein sequence |
