AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86981

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86981.t1 MTR_2g104500 82.105 285 38 4 1 282 1 275 5.21e-166 461
MS.gene86981.t1 MTR_2g104530 63.423 298 70 9 1 281 1 276 8.55e-116 334
MS.gene86981.t1 MTR_2g104550 43.046 302 143 11 1 278 15 311 1.47e-59 192
MS.gene86981.t1 MTR_2g104490 42.617 298 142 9 2 277 16 306 2.17e-58 189
MS.gene86981.t1 MTR_4g009540 43.277 238 124 4 50 279 97 331 1.39e-52 174
MS.gene86981.t1 MTR_2g104490 42.294 279 132 9 2 258 16 287 2.50e-51 170
MS.gene86981.t1 MTR_4g009110 42.324 241 126 3 50 279 112 350 5.45e-51 171
MS.gene86981.t1 MTR_8g009020 48.087 183 87 4 103 279 143 323 1.82e-44 154
MS.gene86981.t1 MTR_4g009540 41.935 217 115 4 50 258 97 310 1.05e-43 151
MS.gene86981.t1 MTR_2g104560 44.693 179 88 4 104 279 55 225 1.85e-41 143
MS.gene86981.t1 MTR_2g104590 43.678 174 77 3 101 274 67 219 3.12e-34 124
MS.gene86981.t1 MTR_5g014600 39.785 186 102 2 103 278 149 334 3.16e-34 127
MS.gene86981.t1 MTR_8g069740 35.185 270 145 8 9 257 5 265 1.98e-33 123
MS.gene86981.t1 MTR_5g014520 39.106 179 106 1 96 274 150 325 2.48e-33 124
MS.gene86981.t1 MTR_7g080780 37.021 235 124 5 54 279 97 316 8.28e-32 120
MS.gene86981.t1 MTR_7g080780 37.021 235 124 5 54 279 110 329 9.53e-32 120
MS.gene86981.t1 MTR_0250s0040 36.517 178 108 2 103 279 88 261 2.24e-31 117
MS.gene86981.t1 MTR_2g010450 36.364 187 112 3 89 274 166 346 3.27e-31 119
MS.gene86981.t1 MTR_5g014640 34.862 218 132 4 62 278 115 323 7.76e-31 117
MS.gene86981.t1 MTR_2g104650 35.789 285 141 9 8 274 4 264 8.71e-31 116
MS.gene86981.t1 MTR_0011s0260 37.640 178 106 3 103 279 183 356 2.38e-30 117
MS.gene86981.t1 MTR_6g047570 33.197 244 147 4 43 279 98 332 4.73e-30 115
MS.gene86981.t1 MTR_4g092700 36.628 172 106 1 103 274 158 326 5.18e-30 115
MS.gene86981.t1 MTR_0011s0210 35.028 177 112 1 103 279 183 356 1.08e-29 115
MS.gene86981.t1 MTR_0246s0020 34.746 236 129 7 67 279 142 375 1.74e-29 115
MS.gene86981.t1 MTR_4g097940 37.222 180 106 2 99 277 151 324 3.05e-28 110
MS.gene86981.t1 MTR_4g066460 35.149 202 113 5 83 279 137 325 3.33e-28 110
MS.gene86981.t1 MTR_8g009010 41.040 173 62 3 103 274 134 267 3.58e-28 110
MS.gene86981.t1 MTR_5g014560 35.204 196 119 2 89 279 112 304 3.87e-27 107
MS.gene86981.t1 MTR_4g067010 34.715 193 118 4 87 279 133 317 3.97e-27 107
MS.gene86981.t1 MTR_0246s0050 30.603 232 145 4 49 279 101 317 1.35e-26 106
MS.gene86981.t1 MTR_4g098035 30.603 232 145 4 49 279 122 338 1.66e-26 106
MS.gene86981.t1 MTR_4g009510 38.323 167 84 4 114 277 53 203 3.53e-26 102
MS.gene86981.t1 MTR_7g050350 41.216 148 62 3 133 274 2 130 6.64e-26 99.8
MS.gene86981.t1 MTR_7g451650 41.216 148 62 3 133 274 2 130 6.64e-26 99.8
MS.gene86981.t1 MTR_7g451650 41.333 150 63 3 131 274 62 192 7.48e-26 101
MS.gene86981.t1 MTR_4g097920 36.111 180 108 2 99 277 136 309 2.48e-25 102
MS.gene86981.t1 MTR_4g097920 36.111 180 108 2 99 277 151 324 2.87e-25 102
MS.gene86981.t1 MTR_0246s0020 34.101 217 118 6 67 260 142 356 4.82e-25 102
MS.gene86981.t1 MTR_4g097950 35.079 191 100 3 103 279 70 250 2.19e-23 96.3
MS.gene86981.t1 MTR_4g009420 37.267 161 79 3 114 274 54 192 8.01e-23 93.6
MS.gene86981.t1 MTR_7g451650 42.029 138 55 3 131 262 62 180 2.86e-22 91.7
MS.gene86981.t1 MTR_4g066380 33.668 199 115 3 89 279 133 322 5.43e-22 94.0
MS.gene86981.t1 MTR_4g009420 37.107 159 73 4 114 267 54 190 3.26e-19 84.0
MS.gene86981.t1 MTR_5g014550 34.615 130 74 2 132 257 53 175 6.00e-14 69.3
MS.gene86981.t1 MTR_8g098275 24.883 213 123 6 78 258 279 486 7.33e-12 65.9
MS.gene86981.t1 MTR_8g098275 24.883 213 123 6 78 258 372 579 9.06e-12 65.5
MS.gene86981.t1 MTR_1g072320 24.615 195 119 5 101 277 468 652 9.21e-12 65.5
MS.gene86981.t1 MTR_8g098275 24.883 213 123 6 78 258 215 422 1.14e-11 65.1
MS.gene86981.t1 MTR_6g047550 31.852 135 79 3 43 170 112 240 3.02e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86981.t1 AT4G37850 39.205 176 103 3 103 277 152 324 5.23e-31 118
MS.gene86981.t1 AT4G37850 39.205 176 103 3 103 277 152 324 5.23e-31 118
MS.gene86981.t1 AT2G22760 37.356 174 106 3 103 274 119 291 1.03e-29 114
MS.gene86981.t1 AT2G22760 36.943 157 97 2 103 258 119 274 1.06e-25 103
MS.gene86981.t1 AT2G22770 34.783 184 108 2 103 274 132 315 1.09e-25 103
MS.gene86981.t1 AT2G22750 34.239 184 96 3 103 273 126 297 8.86e-21 90.1
MS.gene86981.t1 AT2G22750 34.239 184 96 3 103 273 150 321 1.39e-20 90.1
MS.gene86981.t1 AT2G22750 34.239 184 96 3 103 273 154 325 1.44e-20 90.1
MS.gene86981.t1 AT2G22750 34.239 184 95 4 103 273 126 296 2.59e-19 86.3
MS.gene86981.t1 AT2G22750 34.239 184 95 4 103 273 154 324 3.70e-19 86.3
MS.gene86981.t1 AT4G09820 35.766 137 54 5 102 234 362 468 2.34e-13 70.1
MS.gene86981.t1 AT1G63650 29.730 148 89 2 103 235 405 552 2.05e-12 67.4
MS.gene86981.t1 AT1G63650 29.730 148 89 2 103 235 405 552 2.05e-12 67.4
MS.gene86981.t1 AT1G63650 29.730 148 89 2 103 235 405 552 2.05e-12 67.4
MS.gene86981.t1 AT5G41315 27.329 161 97 3 95 235 438 598 9.39e-12 65.5
MS.gene86981.t1 AT5G41315 27.329 161 97 3 95 235 441 601 9.41e-12 65.5
MS.gene86981.t1 AT5G41315 27.329 161 97 3 95 235 433 593 9.54e-12 65.5

Find 51 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAACTCCTTCACTCGTTTC+TGG 0.173101 2.3:+265953 None:intergenic
TAGAGCTTGCATTATCTCTT+TGG 0.266677 2.3:-266852 MS.gene86981:CDS
ATCAATTGTGAGAAGGAAAA+TGG 0.286777 2.3:-265757 MS.gene86981:CDS
ACAACGTGGTGGTTTGGATT+TGG 0.302005 2.3:+266793 None:intergenic
CTGGAGTTGCTCCACGTAAT+TGG 0.302982 2.3:+265972 None:intergenic
TGGCATTCAACACAAAATAT+TGG 0.325928 2.3:-265737 MS.gene86981:CDS
CAAAATGACACTGCTGGATT+TGG 0.327464 2.3:-264953 MS.gene86981:CDS
AGAGATCTAATAATTGCTTT+TGG 0.331987 2.3:+266728 None:intergenic
ATGAATCTTGACAATTATCT+TGG 0.345786 2.3:-265841 MS.gene86981:CDS
AGACTTCCTTTGTGACTTCA+TGG 0.350365 2.3:-266969 MS.gene86981:CDS
GCAATGGCAGCTCCAGCTAC+TGG 0.359273 2.3:+266941 None:intergenic
GACAAAGCTTCTATCGTTGC+TGG 0.389114 2.3:-266000 MS.gene86981:CDS
CCGTTTGGCAATTCAACTCT+TGG 0.391384 2.3:-265667 MS.gene86981:CDS
ACATCAGGAAGCAATTCATT+TGG 0.411022 2.3:+265817 None:intergenic
ATCATGTTGCATCTTTCAAC+AGG 0.411699 2.3:+264911 None:intergenic
TTCTTATTGAGAAGTATCAT+TGG 0.433422 2.3:+265901 None:intergenic
AATGACACTGCTGGATTTGG+CGG 0.437820 2.3:-264950 MS.gene86981:CDS
GACACTTTCACTTTGACATC+AGG 0.450201 2.3:+265802 None:intergenic
TGGATTTGGCGGACAACATA+AGG 0.452584 2.3:-264939 MS.gene86981:CDS
TGTGGGACAACGTGGTGGTT+TGG 0.461838 2.3:+266787 None:intergenic
AGCACTAGCACATTGCCGTT+TGG 0.467143 2.3:-265682 MS.gene86981:CDS
TTCATGGCACAGCCAGTAGC+TGG 0.474815 2.3:-266953 MS.gene86981:CDS
ACGCAATGGGAGAGAGGAAA+AGG 0.480162 2.3:-266700 MS.gene86981:CDS
TTGGATTTGGACATCACAGC+TGG 0.517868 2.3:+266806 None:intergenic
GTGGACGGAGGAAGAAGCTC+CGG 0.531427 2.3:+266881 None:intergenic
AATGCAAGCTCTAGAGGATG+TGG 0.535694 2.3:+266862 None:intergenic
AGATCTCTAGATCACGCAAT+GGG 0.542864 2.3:-266713 MS.gene86981:CDS
AAAGGAAGTCTTCATCTTCG+TGG 0.550705 2.3:+266981 None:intergenic
AAGATTCATTTCACTAGATG+TGG 0.558723 2.3:+265855 None:intergenic
AGAGATAATGCAAGCTCTAG+AGG 0.565903 2.3:+266856 None:intergenic
ACGCTCGGTAGCAGCAGCAA+TGG 0.570572 2.3:+266925 None:intergenic
CCAAGAGTTGAATTGCCAAA+CGG 0.575048 2.3:+265667 None:intergenic
TGTAGTCTCTGATGAGTCAC+CGG 0.575944 2.3:-266900 MS.gene86981:CDS
ATTAGTACCTTGTTTGAGCG+AGG 0.577779 2.3:+266632 None:intergenic
AGCTGGTAACAGTATCTTCG+CGG 0.585382 2.3:+266823 None:intergenic
ATCAGAGACTACAATACGCT+CGG 0.591680 2.3:+266910 None:intergenic
CATTTACATCAATTGTGAGA+AGG 0.601314 2.3:-265764 MS.gene86981:CDS
AAACGGCAATGTGCTAGTGC+TGG 0.603776 2.3:+265684 None:intergenic
TGCATACAAAATGACACTGC+TGG 0.605032 2.3:-264959 MS.gene86981:CDS
TATTATTCCTCGCTCAAACA+AGG 0.615369 2.3:-266639 MS.gene86981:intron
TAGATCACGCAATGGGAGAG+AGG 0.615416 2.3:-266706 MS.gene86981:CDS
TAGATCTCTAGATCACGCAA+TGG 0.625523 2.3:-266714 MS.gene86981:CDS
TGGCATAACTATCATCGCCA+AGG 0.626059 2.3:-265647 MS.gene86981:intron
GAAGTACAACAAAACAAGAG+AGG 0.647529 2.3:-265931 MS.gene86981:CDS
TGCTGGCGCAACCAATTACG+TGG 0.650808 2.3:-265983 MS.gene86981:CDS
GACTTCTGAGAATCGAAACA+TGG 0.657951 2.3:-266753 MS.gene86981:CDS
TGATGTCAAAGTGAAAGTGT+CGG 0.659461 2.3:-265800 MS.gene86981:CDS
GCAACTCCAGAAACGAGTGA+AGG 0.675689 2.3:-265959 MS.gene86981:CDS
CAAGCTCTAGAGGATGTGGA+CGG 0.686043 2.3:+266866 None:intergenic
GCTCTAGAGGATGTGGACGG+AGG 0.694196 2.3:+266869 None:intergenic
GCTGTGCCATGAAGTCACAA+AGG 0.700598 2.3:+266963 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAAATACATTAATAATTT+GGG + chr2.3:265885-265904 None:intergenic 10.0%
!! TTGTAAATACATTAATAATT+TGG + chr2.3:265886-265905 None:intergenic 10.0%
!!! TTATTTAATCATTTTTTGAT+AGG + chr2.3:266705-266724 None:intergenic 10.0%
!! TTCAATAGTTAATGAAAATT+TGG + chr2.3:266310-266329 None:intergenic 15.0%
!!! ATTATTTTTTTGATAAGCAA+AGG + chr2.3:265349-265368 None:intergenic 15.0%
!!! CCATTTTAAAACATTTTTTA+AGG + chr2.3:265737-265756 None:intergenic 15.0%
!!! CCTTAAAAAATGTTTTAAAA+TGG - chr2.3:265734-265753 MS.gene86981:CDS 15.0%
!!! TGAAATGAATATGTTTTTAA+GGG - chr2.3:266326-266345 MS.gene86981:intron 15.0%
!!! TTGAAATGAATATGTTTTTA+AGG - chr2.3:266325-266344 MS.gene86981:intron 15.0%
!! AACAACTTTGTAAAACAAAA+TGG + chr2.3:266625-266644 None:intergenic 20.0%
!! TAGAAAATAAAGACCAATAT+AGG - chr2.3:265711-265730 MS.gene86981:CDS 20.0%
!! TTAATCATATATACCACATA+TGG + chr2.3:266676-266695 None:intergenic 20.0%
!!! TATTTATTGCATTAACTTGA+TGG - chr2.3:266925-266944 MS.gene86981:CDS 20.0%
!!! TGGCATTTATATTAGTTTAT+AGG + chr2.3:266656-266675 None:intergenic 20.0%
! ACTAATATAAATGCCATATG+TGG - chr2.3:266660-266679 MS.gene86981:CDS 25.0%
! ATGAATCTTGACAATTATCT+TGG - chr2.3:266081-266100 MS.gene86981:intron 25.0%
! CTCAATAAGAAAAATTCTTG+TGG - chr2.3:266033-266052 MS.gene86981:intron 25.0%
!! AGAGATCTAATAATTGCTTT+TGG + chr2.3:265197-265216 None:intergenic 25.0%
!! TAATTTGGGATGTCTAATAT+CGG + chr2.3:265871-265890 None:intergenic 25.0%
!!! ACATTTTTTAAGGCCTATAT+TGG + chr2.3:265727-265746 None:intergenic 25.0%
!!! TTCTTATTGAGAAGTATCAT+TGG + chr2.3:266024-266043 None:intergenic 25.0%
AAAACATCTCTTCAAATCGT+TGG - chr2.3:265487-265506 MS.gene86981:intron 30.0%
AAGATTCATTTCACTAGATG+TGG + chr2.3:266070-266089 None:intergenic 30.0%
AATGAGACCTAGTAATTTGA+AGG - chr2.3:266492-266511 MS.gene86981:intron 30.0%
AGTAGAATCTACCTTTAAAC+CGG - chr2.3:265844-265863 MS.gene86981:CDS 30.0%
ATCAATTGTGAGAAGGAAAA+TGG - chr2.3:266165-266184 MS.gene86981:intron 30.0%
CATTTACATCAATTGTGAGA+AGG - chr2.3:266158-266177 MS.gene86981:intron 30.0%
CTAGTAATTTGAAGGTTAGT+CGG - chr2.3:266500-266519 MS.gene86981:intron 30.0%
GAATGAATGTTGAGAAGTTT+AGG - chr2.3:266359-266378 MS.gene86981:intron 30.0%
GTATCAACAACAAATGTTGA+GGG + chr2.3:265578-265597 None:intergenic 30.0%
GTGCTACAATAAAAGAATAG+CGG + chr2.3:266780-266799 None:intergenic 30.0%
TGACATAATACCATTCTTCA+GGG - chr2.3:265527-265546 MS.gene86981:intron 30.0%
TGGCATTCAACACAAAATAT+TGG - chr2.3:266185-266204 MS.gene86981:intron 30.0%
TTGACATAATACCATTCTTC+AGG - chr2.3:265526-265545 MS.gene86981:intron 30.0%
! TAGTAATTTGAAGGTTAGTC+GGG - chr2.3:266501-266520 MS.gene86981:intron 30.0%
!! TAACTTGATGGATTACAGAT+GGG - chr2.3:266937-266956 MS.gene86981:CDS 30.0%
!! TTAACTTGATGGATTACAGA+TGG - chr2.3:266936-266955 MS.gene86981:CDS 30.0%
!! TTGTTAACTCGAGTTTTTAG+TGG - chr2.3:266469-266488 MS.gene86981:intron 30.0%
!!! AGAAGTCATCATCTTTTTTG+TGG + chr2.3:265156-265175 None:intergenic 30.0%
!!! GAAGTCATCATCTTTTTTGT+GGG + chr2.3:265155-265174 None:intergenic 30.0%
!!! TTTTTTGATAAGCAAAGGCA+AGG + chr2.3:265344-265363 None:intergenic 30.0%
ACAATAAAAGAATAGCGGTC+CGG + chr2.3:266775-266794 None:intergenic 35.0%
ACATCAGGAAGCAATTCATT+TGG + chr2.3:266108-266127 None:intergenic 35.0%
CACCATATGACAGATTATTC+AGG + chr2.3:266582-266601 None:intergenic 35.0%
CATATTCAAACCCTGAAGAA+TGG + chr2.3:265540-265559 None:intergenic 35.0%
CGACTAACCTTCAAATTACT+AGG + chr2.3:266502-266521 None:intergenic 35.0%
CTCCTGAATAATCTGTCATA+TGG - chr2.3:266577-266596 MS.gene86981:intron 35.0%
CTGAATAATCTGTCATATGG+TGG - chr2.3:266580-266599 MS.gene86981:intron 35.0%
GAAGTACAACAAAACAAGAG+AGG - chr2.3:265991-266010 MS.gene86981:CDS 35.0%
GGTATCAACAACAAATGTTG+AGG + chr2.3:265579-265598 None:intergenic 35.0%
GTAAAACAAAATGGCTCAAG+TGG + chr2.3:266616-266635 None:intergenic 35.0%
TAGAGCTTGCATTATCTCTT+TGG - chr2.3:265070-265089 MS.gene86981:intron 35.0%
TATTATTCCTCGCTCAAACA+AGG - chr2.3:265283-265302 MS.gene86981:intron 35.0%
! TGATGTCAAAGTGAAAGTGT+CGG - chr2.3:266122-266141 MS.gene86981:intron 35.0%
!! AATGAGTGTTGAGTTTGGAA+GGG + chr2.3:265621-265640 None:intergenic 35.0%
AAAGGAAGTCTTCATCTTCG+TGG + chr2.3:264944-264963 None:intergenic 40.0%
ACTACTTTCCCGAAATCATC+CGG - chr2.3:266753-266772 MS.gene86981:CDS 40.0%
AGACTTCCTTTGTGACTTCA+TGG - chr2.3:264953-264972 MS.gene86981:CDS 40.0%
AGATCTCTAGATCACGCAAT+GGG - chr2.3:265209-265228 MS.gene86981:intron 40.0%
ATCAGAGACTACAATACGCT+CGG + chr2.3:265015-265034 None:intergenic 40.0%
ATCGCGAACATAAAATGCAG+TGG + chr2.3:266851-266870 None:intergenic 40.0%
CAAAATGACACTGCTGGATT+TGG - chr2.3:266969-266988 MS.gene86981:CDS 40.0%
CCAAGAGTTGAATTGCCAAA+CGG + chr2.3:266258-266277 None:intergenic 40.0%
CTAATATCGGTCCGGTTTAA+AGG + chr2.3:265858-265877 None:intergenic 40.0%
GACACTTTCACTTTGACATC+AGG + chr2.3:266123-266142 None:intergenic 40.0%
TAGATCTCTAGATCACGCAA+TGG - chr2.3:265208-265227 MS.gene86981:intron 40.0%
TGCATACAAAATGACACTGC+TGG - chr2.3:266963-266982 MS.gene86981:CDS 40.0%
! AGAGATAATGCAAGCTCTAG+AGG + chr2.3:265069-265088 None:intergenic 40.0%
! ATTAGTACCTTGTTTGAGCG+AGG + chr2.3:265293-265312 None:intergenic 40.0%
! GACTTCTGAGAATCGAAACA+TGG - chr2.3:265169-265188 MS.gene86981:intron 40.0%
! TAATTTGAAGGTTAGTCGGG+AGG - chr2.3:266504-266523 MS.gene86981:intron 40.0%
!! AAATTTGGTGATGCGAACCT+TGG + chr2.3:266295-266314 None:intergenic 40.0%
!! CAATGAGTGTTGAGTTTGGA+AGG + chr2.3:265622-265641 None:intergenic 40.0%
!! CTTGCAATGAGTGTTGAGTT+TGG + chr2.3:265626-265645 None:intergenic 40.0%
!!! ATCTTTTTTGTGGGACAACG+TGG + chr2.3:265146-265165 None:intergenic 40.0%
AAGGGAAGAATGAGGAGGTT+TGG + chr2.3:265603-265622 None:intergenic 45.0%
AATAGCGGTCCGGATGATTT+CGG + chr2.3:266765-266784 None:intergenic 45.0%
AATGACACTGCTGGATTTGG+CGG - chr2.3:266972-266991 MS.gene86981:CDS 45.0%
AGCTGGTAACAGTATCTTCG+CGG + chr2.3:265102-265121 None:intergenic 45.0%
CCGTTTGGCAATTCAACTCT+TGG - chr2.3:266255-266274 MS.gene86981:intron 45.0%
CTAACTCCTTCACTCGTTTC+TGG + chr2.3:265972-265991 None:intergenic 45.0%
GACAAAGCTTCTATCGTTGC+TGG - chr2.3:265922-265941 MS.gene86981:CDS 45.0%
GAGTTTGGAAGGGAAGAATG+AGG + chr2.3:265611-265630 None:intergenic 45.0%
GCGAACATAAAATGCAGTGG+TGG + chr2.3:266848-266867 None:intergenic 45.0%
TGGCATAACTATCATCGCCA+AGG - chr2.3:266275-266294 MS.gene86981:intron 45.0%
TGGGATGTCTAATATCGGTC+CGG + chr2.3:265866-265885 None:intergenic 45.0%
TGTAGTCTCTGATGAGTCAC+CGG - chr2.3:265022-265041 MS.gene86981:intron 45.0%
TTGGATTTGGACATCACAGC+TGG + chr2.3:265119-265138 None:intergenic 45.0%
TTTGGAAGGGAAGAATGAGG+AGG + chr2.3:265608-265627 None:intergenic 45.0%
! ACAACGTGGTGGTTTGGATT+TGG + chr2.3:265132-265151 None:intergenic 45.0%
! TGGATTTGGCGGACAACATA+AGG - chr2.3:266983-267002 MS.gene86981:CDS 45.0%
!! AATGCAAGCTCTAGAGGATG+TGG + chr2.3:265063-265082 None:intergenic 45.0%
!!! TTTTTTGTGGGACAACGTGG+TGG + chr2.3:265143-265162 None:intergenic 45.0%
!! AAAAAAAAAAAACATAAAAT+AGG + chr2.3:266823-266842 None:intergenic 5.0%
!! AAAAATGATTAAATAATTAA+AGG - chr2.3:266709-266728 MS.gene86981:CDS 5.0%
AAACGGCAATGTGCTAGTGC+TGG + chr2.3:266241-266260 None:intergenic 50.0%
AATAGGACCCTTGCCATGCA+TGG + chr2.3:266806-266825 None:intergenic 50.0%
ACGCAATGGGAGAGAGGAAA+AGG - chr2.3:265222-265241 MS.gene86981:intron 50.0%
AGCACTAGCACATTGCCGTT+TGG - chr2.3:266240-266259 MS.gene86981:intron 50.0%
ATAGCGGTCCGGATGATTTC+GGG + chr2.3:266764-266783 None:intergenic 50.0%
ATAGGACCCTTGCCATGCAT+GGG + chr2.3:266805-266824 None:intergenic 50.0%
ATTGTAGCACCTCCCATGCA+TGG - chr2.3:266790-266809 MS.gene86981:CDS 50.0%
CTGGAGTTGCTCCACGTAAT+TGG + chr2.3:265953-265972 None:intergenic 50.0%
GCAACTCCAGAAACGAGTGA+AGG - chr2.3:265963-265982 MS.gene86981:CDS 50.0%
GCTGTGCCATGAAGTCACAA+AGG + chr2.3:264962-264981 None:intergenic 50.0%
GGAAGAATGAGGAGGTTTGG+TGG + chr2.3:265600-265619 None:intergenic 50.0%
TAGATCACGCAATGGGAGAG+AGG - chr2.3:265216-265235 MS.gene86981:intron 50.0%
!! CAAGCTCTAGAGGATGTGGA+CGG + chr2.3:265059-265078 None:intergenic 50.0%
TGCTGGCGCAACCAATTACG+TGG - chr2.3:265939-265958 MS.gene86981:CDS 55.0%
TGTGGGACAACGTGGTGGTT+TGG + chr2.3:265138-265157 None:intergenic 55.0%
TTCATGGCACAGCCAGTAGC+TGG - chr2.3:264969-264988 MS.gene86981:CDS 55.0%
ACGCTCGGTAGCAGCAGCAA+TGG + chr2.3:265000-265019 None:intergenic 60.0%
AGCACCTCCCATGCATGGCA+AGG - chr2.3:266795-266814 MS.gene86981:CDS 60.0%
GCAATGGCAGCTCCAGCTAC+TGG + chr2.3:264984-265003 None:intergenic 60.0%
GCACCTCCCATGCATGGCAA+GGG - chr2.3:266796-266815 MS.gene86981:CDS 60.0%
GTGGACGGAGGAAGAAGCTC+CGG + chr2.3:265044-265063 None:intergenic 60.0%
!! GCTCTAGAGGATGTGGACGG+AGG + chr2.3:265056-265075 None:intergenic 60.0%
GGACCCTTGCCATGCATGGG+AGG + chr2.3:266802-266821 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 264912 267032 264912 ID=MS.gene86981
chr2.3 mRNA 264912 267032 264912 ID=MS.gene86981.t1;Parent=MS.gene86981
chr2.3 exon 266640 267032 266640 ID=MS.gene86981.t1.exon1;Parent=MS.gene86981.t1
chr2.3 CDS 266640 267032 266640 ID=cds.MS.gene86981.t1;Parent=MS.gene86981.t1
chr2.3 exon 265648 266025 265648 ID=MS.gene86981.t1.exon2;Parent=MS.gene86981.t1
chr2.3 CDS 265648 266025 265648 ID=cds.MS.gene86981.t1;Parent=MS.gene86981.t1
chr2.3 exon 264912 264989 264912 ID=MS.gene86981.t1.exon3;Parent=MS.gene86981.t1
chr2.3 CDS 264912 264989 264912 ID=cds.MS.gene86981.t1;Parent=MS.gene86981.t1
Gene Sequence

>MS.gene86981

ATGGATGTGGAATATTTGTTAGAGATGTGCCACGAAGATGAAGACTTCCTTTGTGACTTCATGGCACAGCCAGTAGCTGGAGCTGCCATTGCTGCTGCTACCGAGCGTATTGTAGTCTCTGATGAGTCACCGGAGCTTCTTCCTCCGTCCACATCCTCTAGAGCTTGCATTATCTCTTTGGATAATTCCGCGAAGATACTGTTACCAGCTGTGATGTCCAAATCCAAACCACCACGTTGTCCCACAAAAAAGATGATGACTTCTGAGAATCGAAACATGGAACCAAAAGCAATTATTAGATCTCTAGATCACGCAATGGGAGAGAGGAAAAGGAGACTTGAACTTGCACACAAATTCATAGAGCTTTCAGCTATTATTCCTCGCTCAAACAAGGTACTAATATTTTCACAAATTATTTACTCATTTATTCCTTGCCTTTGCTTATCAAAAAAATAATATGAAATCAGCAGACAGATGAACTTGTTATGAGATAAATACTATTCTCAGCTTAGTTTGTTAGATTTTATTGTGAAGTTTGTAATTATGAGAACTAAGTTTGTTAGATCTGTATGAACAAAACATCTCTTCAAATCGTTGGTTGATGAACAAAATCGTTGACATAATACCATTCTTCAGGGTTTGAATATGTTGTTGTTAACTAAACCCTCAACATTTGTTGTTGATACCACCAAACCTCCTCATTCTTCCCTTCCAAACTCAACACTCATTGCAAGAAAAATTTGAAAAATATATTTCTTTGACAAAAATTTGAAAAATATGTTAAAAAAATTGTTTAGTATAGAAAATAAAGACCAATATAGGCCTTAAAAAATGTTTTAAAATGGACTTTTATTAATTTTATTTAAAAAATGAATTTTATGATAAAAAAAAGTGTAGAACAAAAACAAAATTCTATTAAAATTAATTTAAAAAGTAGAATCTACCTTTAAACCGGACCGATATTAGACATCCCAAATTATTAATGTATTTACAATTTTTTTGTATAGACAGACAAAGCTTCTATCGTTGCTGGCGCAACCAATTACGTGGAGCAACTCCAGAAACGAGTGAAGGAGTTAGAAGTACAACAAAACAAGAGAGGTAAAGAACCAATGATACTTCTCAATAAGAAAAATTCTTGTGGAGTTTCACGTACCACATCTAGTGAAATGAATCTTGACAATTATCTTGGACCAAATGAATTGCTTCCTGATGTCAAAGTGAAAGTGTCGGAGAATAATATACTCATTTACATCAATTGTGAGAAGGAAAATGGCATTCAACACAAAATATTGGACATGCTTCAAAATCTTCATTTGTTTGTCACCAGCACTAGCACATTGCCGTTTGGCAATTCAACTCTTGGCATAACTATCATCGCCAAGGTTCGCATCACCAAATTTTCATTAACTATTGAAATGAATATGTTTTTAAGGGAAATGTTGTTGAATGAATGTTGAGAAGTTTAGGATGTTTAAATATCATTTTTTTTTACAAGTAATTAATATAATTGTTTTCATGAAAATAGTTTAAATATAGTCATCGTTAATTTTATTTTTGTTAACTCGAGTTTTTAGTGGAATGAGACCTAGTAATTTGAAGGTTAGTCGGGAGGTTAGTAAAGTTTAGCTAAAGATTGTTCTATTTAGTTATCAAACTCAAATTCTCCTGAATAATCTGTCATATGGTGGAGTTAATTAACCACTTGAGCCATTTTGTTTTACAAAGTTGTTAAGTTATTCCTATAAACTAATATAAATGCCATATGTGGTATATATGATTAATTAATTCCTATCAAAAAATGATTAAATAATTAAAGGATTATGCTGTTTAAAGTGAAAACTACTTTCCCGAAATCATCCGGACCGCTATTCTTTTATTGTAGCACCTCCCATGCATGGCAAGGGTCCTATTTTATGTTTTTTTTTTTTTGCCACCACTGCATTTTATGTTCGCGATATATTCTTGAGAAACTTGCTCGCGACAAATAGCATTGTCGATAATTAAACAATGTATTTATTGCATTAACTTGATGGATTACAGATGGGTGATGCATACAAAATGACACTGCTGGATTTGGCGGACAACATAAGGCAATTCCTGTTGAAAGATGCAACATGA
Protein sequence

>MS.gene86981.t1

MDVEYLLEMCHEDEDFLCDFMAQPVAGAAIAAATERIVVSDESPELLPPSTSSRACIISLDNSAKILLPAVMSKSKPPRCPTKKMMTSENRNMEPKAIIRSLDHAMGERKRRLELAHKFIELSAIIPRSNKTDKASIVAGATNYVEQLQKRVKELEVQQNKRGKEPMILLNKKNSCGVSRTTSSEMNLDNYLGPNELLPDVKVKVSENNILIYINCEKENGIQHKILDMLQNLHLFVTSTSTLPFGNSTLGITIIAKMGDAYKMTLLDLADNIRQFLLKDAT