Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87213.t1 | XP_003628012.1 | 94.3 | 545 | 30 | 1 | 1 | 544 | 24 | 568 | 2.60E-277 | 964.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87213.t1 | Q9ZVJ3 | 54.1 | 538 | 242 | 2 | 1 | 538 | 18 | 550 | 4.9e-147 | 522.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87213.t1 | G7LID1 | 94.3 | 545 | 30 | 1 | 1 | 544 | 24 | 568 | 1.9e-277 | 964.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene87213 | MS.gene64957 | PPI |
| MS.gene87213 | MS.gene75603 | PPI |
| MS.gene87213 | MS.gene63822 | PPI |
| MS.gene87213 | MS.gene045269 | PPI |
| MS.gene87213 | MS.gene066834 | PPI |
| MS.gene87213 | MS.gene63824 | PPI |
| MS.gene87213 | MS.gene94838 | PPI |
| MS.gene87213 | MS.gene81433 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87213.t1 | MTR_8g041400 | 96.147 | 545 | 20 | 1 | 1 | 544 | 24 | 568 | 0.0 | 1064 |
| MS.gene87213.t1 | MTR_8g023220 | 72.065 | 494 | 105 | 7 | 1 | 483 | 25 | 496 | 0.0 | 646 |
| MS.gene87213.t1 | MTR_5g093860 | 49.639 | 554 | 262 | 7 | 2 | 544 | 26 | 573 | 1.51e-170 | 495 |
| MS.gene87213.t1 | MTR_1g038840 | 41.536 | 573 | 298 | 8 | 2 | 541 | 27 | 595 | 3.45e-136 | 408 |
| MS.gene87213.t1 | MTR_6g061690 | 41.652 | 557 | 288 | 9 | 1 | 541 | 26 | 561 | 1.07e-131 | 400 |
| MS.gene87213.t1 | MTR_1g076700 | 41.848 | 552 | 300 | 5 | 1 | 541 | 25 | 566 | 2.16e-131 | 400 |
| MS.gene87213.t1 | MTR_1g038840 | 41.145 | 559 | 292 | 8 | 16 | 541 | 1 | 555 | 3.40e-129 | 389 |
| MS.gene87213.t1 | MTR_3g085440 | 39.332 | 539 | 308 | 6 | 1 | 522 | 29 | 565 | 2.15e-118 | 362 |
| MS.gene87213.t1 | MTR_7g075220 | 40.230 | 522 | 296 | 6 | 1 | 520 | 335 | 842 | 2.39e-110 | 352 |
| MS.gene87213.t1 | MTR_3g085440 | 41.626 | 406 | 232 | 3 | 1 | 403 | 29 | 432 | 1.04e-95 | 298 |
| MS.gene87213.t1 | MTR_8g032090 | 75.229 | 109 | 23 | 1 | 208 | 316 | 190 | 294 | 2.54e-43 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87213.t1 | AT2G38720 | 54.647 | 538 | 239 | 2 | 1 | 538 | 18 | 550 | 0.0 | 566 |
| MS.gene87213.t1 | AT5G55230 | 50.362 | 552 | 257 | 7 | 2 | 541 | 26 | 572 | 7.41e-170 | 494 |
| MS.gene87213.t1 | AT5G55230 | 50.362 | 552 | 257 | 7 | 2 | 541 | 26 | 572 | 7.41e-170 | 494 |
| MS.gene87213.t1 | AT4G26760 | 48.263 | 547 | 270 | 7 | 2 | 541 | 26 | 566 | 5.83e-164 | 478 |
| MS.gene87213.t1 | AT5G55230 | 48.021 | 581 | 256 | 8 | 2 | 541 | 26 | 601 | 1.40e-163 | 479 |
| MS.gene87213.t1 | AT1G14690 | 39.469 | 565 | 312 | 6 | 2 | 541 | 28 | 587 | 1.90e-130 | 393 |
| MS.gene87213.t1 | AT1G14690 | 39.469 | 565 | 312 | 6 | 2 | 541 | 28 | 587 | 1.90e-130 | 393 |
| MS.gene87213.t1 | AT2G01910 | 38.529 | 571 | 313 | 9 | 2 | 541 | 28 | 591 | 5.07e-124 | 377 |
| MS.gene87213.t1 | AT5G51600 | 42.402 | 533 | 296 | 4 | 1 | 525 | 25 | 554 | 3.54e-121 | 373 |
| MS.gene87213.t1 | AT2G01910 | 38.061 | 557 | 307 | 9 | 16 | 541 | 1 | 550 | 2.38e-117 | 358 |
| MS.gene87213.t1 | AT1G27920 | 38.606 | 531 | 313 | 7 | 1 | 520 | 29 | 557 | 2.39e-117 | 358 |
| MS.gene87213.t1 | AT3G60840 | 40.286 | 489 | 281 | 4 | 2 | 484 | 26 | 509 | 6.29e-108 | 337 |
| MS.gene87213.t1 | AT5G62250 | 36.023 | 533 | 316 | 7 | 1 | 510 | 19 | 549 | 2.46e-88 | 283 |
| MS.gene87213.t1 | AT5G62250 | 36.023 | 533 | 316 | 7 | 1 | 510 | 19 | 549 | 2.46e-88 | 283 |
| MS.gene87213.t1 | AT1G27920 | 38.220 | 382 | 230 | 4 | 1 | 378 | 29 | 408 | 1.60e-80 | 258 |
Find 105 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGTCAGCTTTGTGCTTTC+TGG | 0.060475 | 8.4:-58081742 | None:intergenic |
| CAGCACATATCTGTGATATT+TGG | 0.134029 | 8.4:-58082783 | None:intergenic |
| CACTAATTCCTTCATCTTAC+TGG | 0.215318 | 8.4:-58084103 | None:intergenic |
| GACACTCTTGCTAGATTAAC+TGG | 0.233705 | 8.4:+58083172 | MS.gene87213:CDS |
| GCTCTTTGGCCTTCCTGATT+TGG | 0.252496 | 8.4:-58085044 | None:intergenic |
| CTGTAATGTCAATTGATATT+TGG | 0.269375 | 8.4:+58083080 | MS.gene87213:CDS |
| GGTAGAGTTATGGGATCTTA+TGG | 0.272997 | 8.4:+58083847 | MS.gene87213:CDS |
| AGGTGATATGGGATGAGATT+GGG | 0.295541 | 8.4:+58081637 | None:intergenic |
| AGCACATATCTGTGATATTT+GGG | 0.310218 | 8.4:-58082782 | None:intergenic |
| AATCGATATAGTGCAGTAAA+AGG | 0.314190 | 8.4:+58086340 | MS.gene87213:CDS |
| CAACATCAGACCTGTCTTAG+AGG | 0.324196 | 8.4:+58082708 | MS.gene87213:CDS |
| CAGAGGAGTTCACATGGATA+TGG | 0.329723 | 8.4:+58084158 | MS.gene87213:CDS |
| TCTGCTTAGAGCTTGCTCTT+TGG | 0.334371 | 8.4:-58085058 | None:intergenic |
| TAGGTGATATGGGATGAGAT+TGG | 0.350035 | 8.4:+58081636 | None:intergenic |
| CGCCGAATGCATACTCCTTC+AGG | 0.367526 | 8.4:+58088903 | MS.gene87213:CDS |
| CATACTCCTTCAGGCCGTTA+TGG | 0.373633 | 8.4:+58088912 | MS.gene87213:CDS |
| AAAGCATGGGAGACGGAAAA+AGG | 0.378834 | 8.4:+58087798 | MS.gene87213:CDS |
| CGAAAGATGATTCTGTTTCA+AGG | 0.393855 | 8.4:+58089009 | MS.gene87213:CDS |
| ATCTTGATCCGTGACACCTT+TGG | 0.398395 | 8.4:-58082827 | None:intergenic |
| AGACAATATGCTTCTTCAAC+TGG | 0.410346 | 8.4:+58081677 | MS.gene87213:CDS |
| AAGAAGAGAAGCGGAAATCT+CGG | 0.411004 | 8.4:+58087969 | MS.gene87213:CDS |
| TCTTAGACAGGGTGGAGAAA+TGG | 0.417985 | 8.4:+58085088 | MS.gene87213:CDS |
| TTTGATGAACCGCTCAAGCT+TGG | 0.433393 | 8.4:-58083124 | None:intergenic |
| GGGTTGATTGTGCGTTCTCA+CGG | 0.439222 | 8.4:+58081820 | MS.gene87213:CDS |
| AAAGCATGGATGACCAAATC+AGG | 0.439242 | 8.4:+58085031 | MS.gene87213:CDS |
| TTCTGCTGATGTCATTGAGC+AGG | 0.440289 | 8.4:+58083958 | MS.gene87213:CDS |
| GGTGATATGGGATGAGATTG+GGG | 0.441760 | 8.4:+58081638 | None:intergenic |
| GTATGCATTCGGCGCCCGTT+TGG | 0.451447 | 8.4:-58088894 | None:intergenic |
| TGTACCAGATTCTATGAGGC+TGG | 0.452881 | 8.4:-58084208 | None:intergenic |
| AGTCATGTTAACACTCTAGC+TGG | 0.457363 | 8.4:+58088867 | MS.gene87213:CDS |
| GGTTCCGTTGTTGAATAGCT+TGG | 0.457433 | 8.4:+58087916 | MS.gene87213:intron |
| CTGATATTTCTACTTCACTT+GGG | 0.459612 | 8.4:+58081799 | MS.gene87213:CDS |
| TTGATGAACCGCTCAAGCTT+GGG | 0.460281 | 8.4:-58083123 | None:intergenic |
| TCATGAACTTCAAAATGAAA+AGG | 0.462868 | 8.4:+58082903 | MS.gene87213:CDS |
| GTAGAGCAAGAAGCAATGTT+TGG | 0.465175 | 8.4:+58088807 | MS.gene87213:CDS |
| CTCTAGCTGGGACACCAAAC+GGG | 0.465360 | 8.4:+58088880 | MS.gene87213:CDS |
| ACTCTAGCTGGGACACCAAA+CGG | 0.465702 | 8.4:+58088879 | MS.gene87213:CDS |
| AGCTTGAGCGGTTCATCAAA+TGG | 0.470037 | 8.4:+58083127 | MS.gene87213:CDS |
| CTGACCAGCCTCATAGAATC+TGG | 0.471522 | 8.4:+58084204 | MS.gene87213:CDS |
| AATACCTTTCTTATACGAAA+AGG | 0.473135 | 8.4:+58087821 | MS.gene87213:CDS |
| GGTTGTTCTTTCATTAAAGC+AGG | 0.480728 | 8.4:+58083195 | MS.gene87213:CDS |
| TCAGGCCGTTATGGAACATC+AGG | 0.485302 | 8.4:+58088921 | MS.gene87213:CDS |
| CCTTGACATTTATTACAGAA+AGG | 0.489257 | 8.4:+58081710 | MS.gene87213:CDS |
| GATACCAATTGATGAGCAAA+AGG | 0.491410 | 8.4:+58083871 | MS.gene87213:CDS |
| GACTTCAAGTCCTCTAAGAC+AGG | 0.494481 | 8.4:-58082718 | None:intergenic |
| GCAGAAGAGATATCTTAGAC+AGG | 0.496605 | 8.4:+58085076 | MS.gene87213:CDS |
| TAATCTAGTAACATGGCTAA+AGG | 0.505095 | 8.4:-58083894 | None:intergenic |
| TTTGAAAGCCAGTAAGATGA+AGG | 0.505287 | 8.4:+58084095 | MS.gene87213:CDS |
| GGCCTGAAGGAGTATGCATT+CGG | 0.510263 | 8.4:-58088905 | None:intergenic |
| GCAAAGGATCGCAGAGAAAG+TGG | 0.511647 | 8.4:+58088945 | MS.gene87213:CDS |
| CATCAGCAGAAAGGCCACCT+TGG | 0.513308 | 8.4:-58083946 | None:intergenic |
| GGTTGATTGTGCGTTCTCAC+GGG | 0.513635 | 8.4:+58081821 | MS.gene87213:CDS |
| AGAGGACTTGAAGTCAAAGA+AGG | 0.519584 | 8.4:+58082726 | MS.gene87213:CDS |
| AGCTGACTTAAATAAGTGGT+TGG | 0.526897 | 8.4:+58081755 | MS.gene87213:CDS |
| GAAAGATGATTCTGTTTCAA+GGG | 0.527378 | 8.4:+58089010 | MS.gene87213:CDS |
| ATCTGACCATGGAGAAATTG+AGG | 0.531927 | 8.4:+58082863 | MS.gene87213:CDS |
| GTCTGAATTGCTTCAAAGCA+TGG | 0.532297 | 8.4:+58085017 | MS.gene87213:CDS |
| AAAGCAACAAAGGCTACAAA+AGG | 0.537889 | 8.4:+58083219 | MS.gene87213:CDS |
| ACAAAGCTGACTTAAATAAG+TGG | 0.538848 | 8.4:+58081751 | MS.gene87213:CDS |
| GTGGTTAGACGAATATGAAA+GGG | 0.539184 | 8.4:+58085134 | MS.gene87213:CDS |
| AGAAGAGAAGCGGAAATCTC+GGG | 0.542673 | 8.4:+58087970 | MS.gene87213:CDS |
| GCAAGAGCAACATAGTTCAC+TGG | 0.543220 | 8.4:-58088987 | None:intergenic |
| GCTGATATTTCTACTTCACT+TGG | 0.543355 | 8.4:+58081798 | MS.gene87213:CDS |
| TGACTGCAAAAGTGAAAGCA+TGG | 0.547854 | 8.4:+58087784 | MS.gene87213:CDS |
| AGTGGTTAGACGAATATGAA+AGG | 0.548441 | 8.4:+58085133 | MS.gene87213:CDS |
| TCTGACCATGGAGAAATTGA+GGG | 0.553832 | 8.4:+58082864 | MS.gene87213:CDS |
| ATGGAAATTTGCTGCTGAGG+AGG | 0.557122 | 8.4:+58085107 | MS.gene87213:CDS |
| TGATATTTCTACTTCACTTG+GGG | 0.559517 | 8.4:+58081800 | MS.gene87213:CDS |
| CAGATATGTGCTGAAATAGC+AGG | 0.563151 | 8.4:+58082793 | MS.gene87213:CDS |
| AAGTTCCCTCAATTTCTCCA+TGG | 0.566896 | 8.4:-58082869 | None:intergenic |
| AACTGAGAGAAGAAGAGAAG+CGG | 0.568057 | 8.4:+58087960 | MS.gene87213:CDS |
| CAGAAGAGATATCTTAGACA+GGG | 0.569954 | 8.4:+58085077 | MS.gene87213:CDS |
| ACATGACTACTCTGGTTCAG+AGG | 0.572958 | 8.4:-58088852 | None:intergenic |
| CTCTAAGACAGGTCTGATGT+TGG | 0.576548 | 8.4:-58082707 | None:intergenic |
| ATGAACCGCTCAAGCTTGGG+TGG | 0.577581 | 8.4:-58083120 | None:intergenic |
| TTGCTGCTGAGGAGGAAAAG+TGG | 0.578971 | 8.4:+58085115 | MS.gene87213:CDS |
| GCTCAATGACATCAGCAGAA+AGG | 0.585522 | 8.4:-58083955 | None:intergenic |
| AAAGAAGGTTGAGAGAGTTG+AGG | 0.586776 | 8.4:+58082741 | MS.gene87213:CDS |
| ACACTCTTGCTAGATTAACT+GGG | 0.589093 | 8.4:+58083173 | MS.gene87213:CDS |
| CTGTAATTAATCTAGTAACA+TGG | 0.596768 | 8.4:-58083901 | None:intergenic |
| GAAATGGAAATTTGCTGCTG+AGG | 0.596896 | 8.4:+58085104 | MS.gene87213:CDS |
| GAGTGTTAACATGACTACTC+TGG | 0.597373 | 8.4:-58088860 | None:intergenic |
| TGGACAGTCCAAAGGTGTCA+CGG | 0.604062 | 8.4:+58082819 | MS.gene87213:CDS |
| AAGAGATATCTTAGACAGGG+TGG | 0.605944 | 8.4:+58085080 | MS.gene87213:CDS |
| GTCATGTTAACACTCTAGCT+GGG | 0.608314 | 8.4:+58088868 | MS.gene87213:CDS |
| TTGCACCTGATGTTCCATAA+CGG | 0.608440 | 8.4:-58088926 | None:intergenic |
| GACTGCAAAAGTGAAAGCAT+GGG | 0.608580 | 8.4:+58087785 | MS.gene87213:CDS |
| GACATCCACCCAAGCTTGAG+CGG | 0.608597 | 8.4:+58083115 | MS.gene87213:CDS |
| CCTTTCTGTAATAAATGTCA+AGG | 0.609027 | 8.4:-58081710 | None:intergenic |
| TGTGCTGAAATAGCAGGCTG+TGG | 0.609516 | 8.4:+58082799 | MS.gene87213:CDS |
| GATGTTCCATAACGGCCTGA+AGG | 0.610540 | 8.4:-58088918 | None:intergenic |
| TTATGGAACATCAGGTGCAA+AGG | 0.616637 | 8.4:+58088929 | MS.gene87213:CDS |
| TAAAGCAGGAAAAGCAACAA+AGG | 0.616713 | 8.4:+58083209 | MS.gene87213:CDS |
| AAAAGTGAAAGCATGGGAGA+CGG | 0.617295 | 8.4:+58087791 | MS.gene87213:CDS |
| AAAGATGATTCTGTTTCAAG+GGG | 0.618097 | 8.4:+58089011 | MS.gene87213:CDS |
| GTTGATGAAGTGTCAATCCA+AGG | 0.619399 | 8.4:+58083929 | MS.gene87213:CDS |
| AATTTACAGAGGAGTTCACA+TGG | 0.631432 | 8.4:+58084152 | MS.gene87213:CDS |
| GCAGGCTGTGGACAGTCCAA+AGG | 0.644700 | 8.4:+58082811 | MS.gene87213:CDS |
| CACTCTTGCTAGATTAACTG+GGG | 0.653182 | 8.4:+58083174 | MS.gene87213:CDS |
| GAACTTGAAGAAATTTACAG+AGG | 0.654777 | 8.4:+58084141 | MS.gene87213:CDS |
| CATGGATGACCAAATCAGGA+AGG | 0.665451 | 8.4:+58085035 | MS.gene87213:CDS |
| TCATCCAAGCTATTCAACAA+CGG | 0.704256 | 8.4:-58087920 | None:intergenic |
| TGATCAATGTGATCTGACCA+TGG | 0.713641 | 8.4:+58082852 | MS.gene87213:CDS |
| GTGTCATTACTAATGCATTG+TGG | 0.740368 | 8.4:-58083154 | None:intergenic |
| GATGAAGTGTCAATCCAAGG+TGG | 0.746045 | 8.4:+58083932 | MS.gene87213:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 58081639 | 58089040 | 58081639 | ID=MS.gene87213 |
| chr8.4 | mRNA | 58081639 | 58089040 | 58081639 | ID=MS.gene87213.t1;Parent=MS.gene87213 |
| chr8.4 | exon | 58081639 | 58081842 | 58081639 | ID=MS.gene87213.t1.exon1;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58081639 | 58081842 | 58081639 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58082679 | 58082924 | 58082679 | ID=MS.gene87213.t1.exon2;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58082679 | 58082924 | 58082679 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58083025 | 58083240 | 58083025 | ID=MS.gene87213.t1.exon3;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58083025 | 58083240 | 58083025 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58083824 | 58083979 | 58083824 | ID=MS.gene87213.t1.exon4;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58083824 | 58083979 | 58083824 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58084066 | 58084225 | 58084066 | ID=MS.gene87213.t1.exon5;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58084066 | 58084225 | 58084066 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58085004 | 58085155 | 58085004 | ID=MS.gene87213.t1.exon6;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58085004 | 58085155 | 58085004 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58086334 | 58086421 | 58086334 | ID=MS.gene87213.t1.exon7;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58086334 | 58086421 | 58086334 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58087769 | 58087842 | 58087769 | ID=MS.gene87213.t1.exon8;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58087769 | 58087842 | 58087769 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58087917 | 58087991 | 58087917 | ID=MS.gene87213.t1.exon9;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58087917 | 58087991 | 58087917 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| chr8.4 | exon | 58088777 | 58089040 | 58088777 | ID=MS.gene87213.t1.exon10;Parent=MS.gene87213.t1 |
| chr8.4 | CDS | 58088777 | 58089040 | 58088777 | ID=cds.MS.gene87213.t1;Parent=MS.gene87213.t1 |
| Gene Sequence |
| Protein sequence |
