AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87270.t1 MTR_2g082640 98.696 230 2 1 1 229 1 230 4.39e-168 462
MS.gene87270.t1 MTR_4g081370 78.571 238 41 4 1 229 1 237 2.69e-125 354
MS.gene87270.t1 MTR_4g081370 87.634 186 22 1 45 229 8 193 3.65e-114 325
MS.gene87270.t1 MTR_2g089000 50.195 257 84 8 9 229 12 260 1.26e-72 222
MS.gene87270.t1 MTR_1g043430 52.273 176 80 1 54 229 75 246 6.18e-58 184
MS.gene87270.t1 MTR_4g075480 51.515 99 47 1 69 167 6 103 4.94e-23 94.4
MS.gene87270.t1 MTR_4g075480 51.515 99 47 1 69 167 34 131 1.28e-22 94.0
MS.gene87270.t1 MTR_1g032600 36.806 144 78 3 32 167 36 174 1.99e-19 85.9
MS.gene87270.t1 MTR_4g108360 33.898 118 74 2 51 168 35 148 4.39e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87270.t1 AT5G54680 69.787 235 64 5 1 229 1 234 2.18e-108 311
MS.gene87270.t1 AT1G51070 62.343 239 67 6 1 229 1 226 5.99e-95 277
MS.gene87270.t1 AT1G51070 65.829 199 57 4 36 229 100 292 3.08e-84 252
MS.gene87270.t1 AT4G14410 52.041 196 77 6 37 229 96 277 2.77e-55 178
MS.gene87270.t1 AT4G14410 52.041 196 77 6 37 229 96 277 2.77e-55 178
MS.gene87270.t1 AT4G14410 52.041 196 77 6 37 229 102 283 3.14e-55 178
MS.gene87270.t1 AT3G23210 52.261 199 87 5 35 229 97 291 4.98e-55 178
MS.gene87270.t1 AT3G23210 52.261 199 87 5 35 229 126 320 1.37e-54 177
MS.gene87270.t1 AT3G19860 45.299 117 59 2 66 178 2 117 1.24e-22 93.6
MS.gene87270.t1 AT3G19860 45.299 117 59 2 66 178 33 148 2.25e-22 93.6
MS.gene87270.t1 AT3G19860 45.299 117 59 2 66 178 55 170 2.39e-22 93.6
MS.gene87270.t1 AT4G36060 38.971 136 72 5 69 199 26 155 1.31e-18 82.4
MS.gene87270.t1 AT4G36060 39.706 136 71 5 69 199 26 155 2.32e-18 81.6
MS.gene87270.t1 AT4G36060 38.971 136 72 5 69 199 44 173 4.41e-18 81.3
MS.gene87270.t1 AT4G36060 39.706 136 71 5 69 199 44 173 4.76e-18 81.3
MS.gene87270.t1 AT3G47640 30.128 156 93 4 73 216 31 182 4.93e-12 63.9
MS.gene87270.t1 AT3G47640 30.128 156 93 4 73 216 31 182 4.93e-12 63.9
MS.gene87270.t1 AT3G47640 30.128 156 93 4 73 216 31 182 4.93e-12 63.9

Find 77 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAAGATTAAAGAGTTAA+AGG 0.125284 2.2:-14423410 MS.gene87270:intron
AATTTGAGTCTTTCAACTTT+TGG 0.132451 2.2:+14423443 None:intergenic
TGAAAGGCATTAACTTGTTT+GGG 0.247330 2.2:+14423064 None:intergenic
ATGAAAGGCATTAACTTGTT+TGG 0.257284 2.2:+14423063 None:intergenic
ATTGATAGGTTTGTTGAATT+AGG 0.284956 2.2:-14423559 MS.gene87270:intron
AGGAGGGTGTGTCAAGAAAC+TGG 0.351738 2.2:+14423122 None:intergenic
ATGTGCTCCTTCCAGCTCTA+AGG 0.373277 2.2:-14423716 MS.gene87270:CDS
GCATTAACTTGTTTGGGGCT+TGG 0.383016 2.2:+14423070 None:intergenic
CTCTGCTCCTAGTATTGAAA+TGG 0.383024 2.2:-14424099 MS.gene87270:intron
GCAAATGCAGCAGGGATTGC+AGG 0.388118 2.2:+14423102 None:intergenic
AAGGAGCCATCGACAGAAAC+AGG 0.397378 2.2:+14424911 None:intergenic
CGGTTGCCAATTTAGGCAAC+TGG 0.406655 2.2:+14422961 None:intergenic
CCGAATGGTGACACAGTTGC+GGG 0.410732 2.2:-14423473 MS.gene87270:CDS
AAGTTGAAAGACTCAAATTC+AGG 0.412450 2.2:-14423439 MS.gene87270:CDS
TGTTGAATTAGGCTCCATCT+TGG 0.436518 2.2:-14423548 MS.gene87270:CDS
TAAGGCTGAGAAGGAGAAGT+TGG 0.437128 2.2:-14423175 MS.gene87270:CDS
GAAGGAGAAGTTGGAGCAGC+AGG 0.442475 2.2:-14423166 MS.gene87270:CDS
ATGCCTTTCATGAGTTATCC+AGG 0.449494 2.2:-14423051 MS.gene87270:CDS
AATTGCCACATGGCAACTCC+TGG 0.456430 2.2:+14423033 None:intergenic
CCCGCAACTGTGTCACCATT+CGG 0.471915 2.2:+14423473 None:intergenic
TGATGAGAAGCAGAGGCTTA+AGG 0.477872 2.2:-14423193 MS.gene87270:CDS
TTTCCCCGGAAAACACCAAT+TGG 0.488899 2.2:-14424964 MS.gene87270:CDS
GAGAGAAGTTGCGGAGGGAT+AGG 0.490114 2.2:-14423687 MS.gene87270:CDS
CCTTGATGAGCAAATGCAGC+AGG 0.490383 2.2:+14423093 None:intergenic
AGTTACCTCTTCTTCGAACC+AGG 0.496718 2.2:+14424038 None:intergenic
TTCTTGTTGCAGAGGTAGAT+CGG 0.503971 2.2:-14423743 MS.gene87270:intron
TCCGAATGGTGACACAGTTG+CGG 0.503977 2.2:-14423474 MS.gene87270:CDS
TTAGGCTCCATCTTGGAGCC+TGG 0.505934 2.2:-14423541 MS.gene87270:CDS
TGTGAGGTATCCACGGCGGC+TGG 0.507559 2.2:+14423003 None:intergenic
GAAATTCCTGTTTCTGTCGA+TGG 0.514909 2.2:-14424917 MS.gene87270:CDS
CAGGAATTTCATCAATCAAA+GGG 0.518186 2.2:+14424930 None:intergenic
GCCATTGGAGAGTTGAGGTG+GGG 0.518653 2.2:+14424874 None:intergenic
AGGGTAATCGAAAAGCCAAT+TGG 0.523695 2.2:+14424949 None:intergenic
CGCCATTGGAGAGTTGAGGT+GGG 0.524275 2.2:+14424873 None:intergenic
ACAGGAATTTCATCAATCAA+AGG 0.524589 2.2:+14424929 None:intergenic
CTCTCGACATGCCTTAGAGC+TGG 0.527575 2.2:+14423705 None:intergenic
CAACCCAACTATGGTTTCCC+CGG 0.528497 2.2:-14424978 None:intergenic
GCAGAGGCTTAAGGCTGAGA+AGG 0.531438 2.2:-14423184 MS.gene87270:CDS
CCTGCTGCATTTGCTCATCA+AGG 0.536277 2.2:-14423093 MS.gene87270:CDS
CACCTCAACTCTCCAATGGC+GGG 0.541239 2.2:-14424871 MS.gene87270:intron
GAGGTATCCACGGCGGCTGG+TGG 0.543817 2.2:+14423006 None:intergenic
CAAAATCAGATACCCGCCAT+TGG 0.547933 2.2:+14424859 None:intergenic
GTGTGTCAAGAAACTGGGTT+GGG 0.557971 2.2:+14423128 None:intergenic
GCTCCATCTTGGAGCCTGGA+AGG 0.560039 2.2:-14423537 MS.gene87270:CDS
AAGAACCTGGTTCGAAGAAG+AGG 0.561194 2.2:-14424043 MS.gene87270:intron
CCGCCATTGGAGAGTTGAGG+TGG 0.568621 2.2:+14424872 None:intergenic
GAAAGGCATTAACTTGTTTG+GGG 0.569345 2.2:+14423065 None:intergenic
CCATTGGAGAGTTGAGGTGG+GGG 0.574009 2.2:+14424875 None:intergenic
TGGCCTTCCAGGCTCCAAGA+TGG 0.580720 2.2:+14423534 None:intergenic
AATGGATGATAGCTCTCTAG+TGG 0.589069 2.2:-14424081 MS.gene87270:CDS
CGACATGCCTTAGAGCTGGA+AGG 0.592843 2.2:+14423709 None:intergenic
CAATTTAGGCAACTGGTGGA+CGG 0.594311 2.2:+14422968 None:intergenic
CCACCTCAACTCTCCAATGG+CGG 0.595417 2.2:-14424872 MS.gene87270:CDS
ATTCATGCCACCAGCCGCCG+TGG 0.596384 2.2:-14423013 MS.gene87270:CDS
AGGCATGTCGAGAGAAGTTG+CGG 0.604709 2.2:-14423696 MS.gene87270:CDS
ATGATCTTGTGAGGTATCCA+CGG 0.609314 2.2:+14422996 None:intergenic
ACTCCTGGATAACTCATGAA+AGG 0.613176 2.2:+14423048 None:intergenic
TCTGATGGTATCAAAGAACC+TGG 0.613566 2.2:-14424056 MS.gene87270:CDS
TTGCCAATTTAGGCAACTGG+TGG 0.613733 2.2:+14422964 None:intergenic
TACCCGCCATTGGAGAGTTG+AGG 0.621755 2.2:+14424869 None:intergenic
AACTTCGTGATGAGAAGCAG+AGG 0.627738 2.2:-14423200 MS.gene87270:CDS
GGTGTGTCAAGAAACTGGGT+TGG 0.631646 2.2:+14423127 None:intergenic
AGCTCTCTAGTGGATTCTGA+TGG 0.634077 2.2:-14424071 MS.gene87270:CDS
ATGGCTCCTTCGCCTTCACG+TGG 0.638692 2.2:-14424898 MS.gene87270:CDS
CTTGATGAGCAAATGCAGCA+GGG 0.642514 2.2:+14423094 None:intergenic
AATGCAGCAGGGATTGCAGG+AGG 0.644570 2.2:+14423105 None:intergenic
ATGCAGCAGGGATTGCAGGA+GGG 0.653807 2.2:+14423106 None:intergenic
CATGTCGAGAGAAGTTGCGG+AGG 0.656044 2.2:-14423693 MS.gene87270:CDS
ATGTCGAGAGAAGTTGCGGA+GGG 0.659997 2.2:-14423692 MS.gene87270:CDS
TCTGATTGATGCTGTCCGAA+TGG 0.662212 2.2:-14423488 MS.gene87270:CDS
TGGTGGCATGAATTGCCACA+TGG 0.684045 2.2:+14423023 None:intergenic
GGAGGGTGTGTCAAGAAACT+GGG 0.689856 2.2:+14423123 None:intergenic
ACGGAGTACATGATCTTGTG+AGG 0.697745 2.2:+14422987 None:intergenic
AAGGCCACCTAAAACAGACA+AGG 0.714338 2.2:-14423518 MS.gene87270:CDS
CGAATGGTGACACAGTTGCG+GGG 0.732614 2.2:-14423472 MS.gene87270:CDS
GTTATCCAGGAGTTGCCATG+TGG 0.743485 2.2:-14423038 MS.gene87270:CDS
ATCTTGTGAGGTATCCACGG+CGG 0.745646 2.2:+14422999 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAAATACAAAAATAT+GGG + chr2.2:14424182-14424201 None:intergenic 10.0%
!! AACTTAGTAAATTAATACTA+TGG + chr2.2:14423804-14423823 None:intergenic 15.0%
!! ACTTAGTAAATTAATACTAT+GGG + chr2.2:14423803-14423822 None:intergenic 15.0%
!! AGTTAAAGTATAAAAATGAA+GGG + chr2.2:14424626-14424645 None:intergenic 15.0%
!! CAAGAAAAAATACAAAAATA+TGG + chr2.2:14424183-14424202 None:intergenic 15.0%
!!! AAAGTTTGAATTTTTAAACT+TGG - chr2.2:14423178-14423197 MS.gene87270:CDS 15.0%
!!! AAATGAAATGATTTAGAATA+TGG + chr2.2:14424603-14424622 None:intergenic 15.0%
!!! AAGTTTGAATTTTTAAACTT+GGG - chr2.2:14423179-14423198 MS.gene87270:CDS 15.0%
!! CAGTTAAAGTATAAAAATGA+AGG + chr2.2:14424627-14424646 None:intergenic 20.0%
!! GTAATATAATTGAGTCAAAT+TGG - chr2.2:14424039-14424058 MS.gene87270:intron 20.0%
!! TATCCACAAATTTGAAAAAA+GGG + chr2.2:14423141-14423160 None:intergenic 20.0%
!! TCAATATTCAGTGTTATATA+AGG + chr2.2:14424689-14424708 None:intergenic 20.0%
!! TTATCCACAAATTTGAAAAA+AGG + chr2.2:14423142-14423161 None:intergenic 20.0%
!!! GTAGTTTTTATAAATTGTTG+TGG - chr2.2:14423226-14423245 MS.gene87270:intron 20.0%
! AAACCCTAAATTAAGAAAAG+GGG + chr2.2:14423107-14423126 None:intergenic 25.0%
! AATAATAAAAGGCAAGATAG+TGG + chr2.2:14424297-14424316 None:intergenic 25.0%
! AGAGAAGATTAAAGAGTTAA+AGG - chr2.2:14424530-14424549 MS.gene87270:intron 25.0%
! AGATTAAAGAGTTAAAGGTA+AGG - chr2.2:14424535-14424554 MS.gene87270:intron 25.0%
! AGTAAATTAATACTATGGGA+TGG + chr2.2:14423799-14423818 None:intergenic 25.0%
! ATAATAAAAGGCAAGATAGT+GGG + chr2.2:14424296-14424315 None:intergenic 25.0%
! ATCCACAAATTTGAAAAAAG+GGG + chr2.2:14423140-14423159 None:intergenic 25.0%
! CTAATATGATTGACATTGAT+AGG - chr2.2:14424367-14424386 MS.gene87270:intron 25.0%
! CTTGTTTAATGAGAAATGAA+AGG + chr2.2:14423939-14423958 None:intergenic 25.0%
! GAAACCCTAAATTAAGAAAA+GGG + chr2.2:14423108-14423127 None:intergenic 25.0%
! GTAAATTAATACTATGGGAT+GGG + chr2.2:14423798-14423817 None:intergenic 25.0%
! TCTTATAGTATACAAGCAAT+AGG + chr2.2:14424150-14424169 None:intergenic 25.0%
! TGAAACCCTAAATTAAGAAA+AGG + chr2.2:14423109-14423128 None:intergenic 25.0%
!! AAATTGCTCTAAATCAATTG+AGG + chr2.2:14424561-14424580 None:intergenic 25.0%
!! AAATTGTTGTGGTTTTCATT+TGG - chr2.2:14423237-14423256 MS.gene87270:intron 25.0%
!! AATTTGAGTCTTTCAACTTT+TGG + chr2.2:14424500-14424519 None:intergenic 25.0%
!! ATTTGAGTCTTTCAACTTTT+GGG + chr2.2:14424499-14424518 None:intergenic 25.0%
!! GACAAGTGTAATTTTTGAAT+TGG - chr2.2:14423314-14423333 MS.gene87270:intron 25.0%
!! TGTTATGTTTTGTGTACTTA+TGG - chr2.2:14423272-14423291 MS.gene87270:intron 25.0%
!!! AATTTTCTTTGAGAGATAGA+AGG - chr2.2:14424576-14424595 MS.gene87270:intron 25.0%
!!! ATTGATAGGTTTGTTGAATT+AGG - chr2.2:14424381-14424400 MS.gene87270:intron 25.0%
AAACCAAAACCTAAAATCCA+AGG + chr2.2:14423738-14423757 None:intergenic 30.0%
AAACCTAAAATCCAAGGTAA+AGG + chr2.2:14423732-14423751 None:intergenic 30.0%
AAAGGAATTGCAACAGTAAT+TGG + chr2.2:14423714-14423733 None:intergenic 30.0%
AAGAGGAGAGGAATAATAAA+AGG + chr2.2:14424308-14424327 None:intergenic 30.0%
AAGGAATTGCAACAGTAATT+GGG + chr2.2:14423713-14423732 None:intergenic 30.0%
AAGTTGAAAGACTCAAATTC+AGG - chr2.2:14424501-14424520 MS.gene87270:intron 30.0%
ACAGGAATTTCATCAATCAA+AGG + chr2.2:14423014-14423033 None:intergenic 30.0%
CAGGAATTTCATCAATCAAA+GGG + chr2.2:14423013-14423032 None:intergenic 30.0%
CTATCATCCATTTCAATACT+AGG + chr2.2:14423851-14423870 None:intergenic 30.0%
GTCAAATTGGCAATTTAACA+TGG - chr2.2:14424052-14424071 MS.gene87270:CDS 30.0%
TAATAAAAGGCAAGATAGTG+GGG + chr2.2:14424295-14424314 None:intergenic 30.0%
TTGAATTCTATCTACTATGC+AGG - chr2.2:14424705-14424724 MS.gene87270:intron 30.0%
! ATTCCTTTACCTTGGATTTT+AGG - chr2.2:14423726-14423745 MS.gene87270:CDS 30.0%
! GTTTTGTGTACTTATGGTTA+TGG - chr2.2:14423278-14423297 MS.gene87270:intron 30.0%
! TTGCCCCTTTTCTTAATTTA+GGG - chr2.2:14423101-14423120 MS.gene87270:CDS 30.0%
! TTTGCCCCTTTTCTTAATTT+AGG - chr2.2:14423100-14423119 MS.gene87270:CDS 30.0%
! TTTTTATTGATGCCACTGAA+CGG + chr2.2:14424112-14424131 None:intergenic 30.0%
!! ATGAAAGGCATTAACTTGTT+TGG + chr2.2:14424880-14424899 None:intergenic 30.0%
!! TGAAAGGCATTAACTTGTTT+GGG + chr2.2:14424879-14424898 None:intergenic 30.0%
!!! TGTATTTTTTCTTGTTGCAG+AGG - chr2.2:14424189-14424208 MS.gene87270:intron 30.0%
!!! TTACCTTGGATTTTAGGTTT+TGG - chr2.2:14423732-14423751 MS.gene87270:CDS 30.0%
AATTGATATGCGTTGAACAC+TGG - chr2.2:14423531-14423550 MS.gene87270:CDS 35.0%
ACAAGCAATAGGATTTGACA+GGG + chr2.2:14424139-14424158 None:intergenic 35.0%
AGGAATTGCAACAGTAATTG+GGG + chr2.2:14423712-14423731 None:intergenic 35.0%
CATCAACAGCTATTGAAACA+AGG + chr2.2:14423403-14423422 None:intergenic 35.0%
CGCAGTATTCCATAATACAA+GGG + chr2.2:14423629-14423648 None:intergenic 35.0%
TACAAGCAATAGGATTTGAC+AGG + chr2.2:14424140-14424159 None:intergenic 35.0%
TCAACTAACTTACAGCTTTG+AGG - chr2.2:14424017-14424036 MS.gene87270:intron 35.0%
TCGCAGTATTCCATAATACA+AGG + chr2.2:14423630-14423649 None:intergenic 35.0%
TGAAGATAATTAGCGATAGC+AGG - chr2.2:14424084-14424103 MS.gene87270:CDS 35.0%
TGTTCTTGAATCTTCTGTTC+TGG - chr2.2:14423347-14423366 MS.gene87270:intron 35.0%
TTGTGTACTTATGGTTATGG+TGG - chr2.2:14423281-14423300 MS.gene87270:intron 35.0%
! TATTCCTCTCCTCTTTTACT+CGG - chr2.2:14424313-14424332 MS.gene87270:intron 35.0%
!! AAAAGCCAATTGGTGTTTTC+CGG + chr2.2:14422984-14423003 None:intergenic 35.0%
!! GAAAGGCATTAACTTGTTTG+GGG + chr2.2:14424878-14424897 None:intergenic 35.0%
!!! TGCCCCTTTTTTCAAATTTG+TGG - chr2.2:14423135-14423154 MS.gene87270:CDS 35.0%
AATGGATGATAGCTCTCTAG+TGG - chr2.2:14423859-14423878 MS.gene87270:intron 40.0%
ACATACACGAACACAAGACA+CGG - chr2.2:14423433-14423452 MS.gene87270:CDS 40.0%
ACTCCTGGATAACTCATGAA+AGG + chr2.2:14424895-14424914 None:intergenic 40.0%
AGGGTAATCGAAAAGCCAAT+TGG + chr2.2:14422994-14423013 None:intergenic 40.0%
ATGCCTTTCATGAGTTATCC+AGG - chr2.2:14424889-14424908 MS.gene87270:CDS 40.0%
CAATTTGCACAAGCAGCTTA+AGG + chr2.2:14423595-14423614 None:intergenic 40.0%
CTGTTGCAATTCCTTTACCT+TGG - chr2.2:14423718-14423737 MS.gene87270:CDS 40.0%
TACCTGCATCCCTTGTATTA+TGG - chr2.2:14423617-14423636 MS.gene87270:intron 40.0%
TCTGATGGTATCAAAGAACC+TGG - chr2.2:14423884-14423903 MS.gene87270:intron 40.0%
TCTTGTGTTCGTGTATGTGT+CGG + chr2.2:14423431-14423450 None:intergenic 40.0%
TGGGTGTAGACTGTGTATTT+GGG - chr2.2:14424336-14424355 MS.gene87270:intron 40.0%
TGTTGAATTAGGCTCCATCT+TGG - chr2.2:14424392-14424411 MS.gene87270:intron 40.0%
TTCCATAATACAAGGGATGC+AGG + chr2.2:14423622-14423641 None:intergenic 40.0%
TTCTTGTTGCAGAGGTAGAT+CGG - chr2.2:14424197-14424216 MS.gene87270:intron 40.0%
! ATCTTCTGTTCTGGATACGT+TGG - chr2.2:14423356-14423375 MS.gene87270:intron 40.0%
! ATGATCTTGTGAGGTATCCA+CGG + chr2.2:14424947-14424966 None:intergenic 40.0%
! ATTGCTGCCTTGTCTGTTTT+AGG + chr2.2:14424432-14424451 None:intergenic 40.0%
! CTCTGCTCCTAGTATTGAAA+TGG - chr2.2:14423841-14423860 MS.gene87270:intron 40.0%
!! AAAGCCAATTGGTGTTTTCC+GGG + chr2.2:14422983-14423002 None:intergenic 40.0%
!! GAAATTCCTGTTTCTGTCGA+TGG - chr2.2:14423023-14423042 MS.gene87270:CDS 40.0%
AACTTCGTGATGAGAAGCAG+AGG - chr2.2:14424740-14424759 MS.gene87270:intron 45.0%
AAGGCCACCTAAAACAGACA+AGG - chr2.2:14424422-14424441 MS.gene87270:intron 45.0%
ACGGAGTACATGATCTTGTG+AGG + chr2.2:14424956-14424975 None:intergenic 45.0%
AGTTACCTCTTCTTCGAACC+AGG + chr2.2:14423905-14423924 None:intergenic 45.0%
CAAAATCAGATACCCGCCAT+TGG + chr2.2:14423084-14423103 None:intergenic 45.0%
CTTGATGAGCAAATGCAGCA+GGG + chr2.2:14424849-14424868 None:intergenic 45.0%
GGGTGTAGACTGTGTATTTG+GGG - chr2.2:14424337-14424356 MS.gene87270:intron 45.0%
GTGGGTGTAGACTGTGTATT+TGG - chr2.2:14424335-14424354 MS.gene87270:intron 45.0%
GTGTGTCAAGAAACTGGGTT+GGG + chr2.2:14424815-14424834 None:intergenic 45.0%
TAAGGCTGAGAAGGAGAAGT+TGG - chr2.2:14424765-14424784 MS.gene87270:intron 45.0%
TCTGATTGATGCTGTCCGAA+TGG - chr2.2:14424452-14424471 MS.gene87270:intron 45.0%
TGATGAGAAGCAGAGGCTTA+AGG - chr2.2:14424747-14424766 MS.gene87270:intron 45.0%
TTTCCCCGGAAAACACCAAT+TGG - chr2.2:14422976-14422995 MS.gene87270:CDS 45.0%
! AAGAACCTGGTTCGAAGAAG+AGG - chr2.2:14423897-14423916 MS.gene87270:intron 45.0%
! CACATGTGTGAGTGTTGTGT+CGG - chr2.2:14423501-14423520 MS.gene87270:CDS 45.0%
! TCTGTTCTGGATACGTTGGT+GGG - chr2.2:14423360-14423379 MS.gene87270:intron 45.0%
! TTCTGTTCTGGATACGTTGG+TGG - chr2.2:14423359-14423378 MS.gene87270:intron 45.0%
!! AAGCCAATTGGTGTTTTCCG+GGG + chr2.2:14422982-14423001 None:intergenic 45.0%
!! AGCTCTCTAGTGGATTCTGA+TGG - chr2.2:14423869-14423888 MS.gene87270:intron 45.0%
!! GCATTAACTTGTTTGGGGCT+TGG + chr2.2:14424873-14424892 None:intergenic 45.0%
AAGGAGCCATCGACAGAAAC+AGG + chr2.2:14423032-14423051 None:intergenic 50.0%
AATTGCCACATGGCAACTCC+TGG + chr2.2:14424910-14424929 None:intergenic 50.0%
AGGCATGTCGAGAGAAGTTG+CGG - chr2.2:14424244-14424263 MS.gene87270:intron 50.0%
ATGTCGAGAGAAGTTGCGGA+GGG - chr2.2:14424248-14424267 MS.gene87270:intron 50.0%
ATGTGCTCCTTCCAGCTCTA+AGG - chr2.2:14424224-14424243 MS.gene87270:intron 50.0%
CCTGCTGCATTTGCTCATCA+AGG - chr2.2:14424847-14424866 MS.gene87270:intron 50.0%
CCTTGATGAGCAAATGCAGC+AGG + chr2.2:14424850-14424869 None:intergenic 50.0%
CTACACCCACCGAGTAAAAG+AGG + chr2.2:14424325-14424344 None:intergenic 50.0%
GGTGTGTCAAGAAACTGGGT+TGG + chr2.2:14424816-14424835 None:intergenic 50.0%
GTTATCCAGGAGTTGCCATG+TGG - chr2.2:14424902-14424921 MS.gene87270:CDS 50.0%
TCCGAATGGTGACACAGTTG+CGG - chr2.2:14424466-14424485 MS.gene87270:intron 50.0%
TGGTGGCATGAATTGCCACA+TGG + chr2.2:14424920-14424939 None:intergenic 50.0%
! AGGAGGGTGTGTCAAGAAAC+TGG + chr2.2:14424821-14424840 None:intergenic 50.0%
! ATCTTGTGAGGTATCCACGG+CGG + chr2.2:14424944-14424963 None:intergenic 50.0%
! CCTCTCCTCTTTTACTCGGT+GGG - chr2.2:14424317-14424336 MS.gene87270:intron 50.0%
! GGAGGGTGTGTCAAGAAACT+GGG + chr2.2:14424820-14424839 None:intergenic 50.0%
! GTGAGTGTTGTGTCGGTGTT+GGG - chr2.2:14423508-14423527 MS.gene87270:CDS 50.0%
! TCCTCTCCTCTTTTACTCGG+TGG - chr2.2:14424316-14424335 MS.gene87270:intron 50.0%
! TGTGAGTGTTGTGTCGGTGT+TGG - chr2.2:14423507-14423526 MS.gene87270:CDS 50.0%
!! GCTGCCTTGTCTGTTTTAGG+TGG + chr2.2:14424429-14424448 None:intergenic 50.0%
!! TCTGTTTTAGGTGGCCTTCC+AGG + chr2.2:14424420-14424439 None:intergenic 50.0%
AATGCAGCAGGGATTGCAGG+AGG + chr2.2:14424838-14424857 None:intergenic 55.0%
ATGCAGCAGGGATTGCAGGA+GGG + chr2.2:14424837-14424856 None:intergenic 55.0%
CACCTCAACTCTCCAATGGC+GGG - chr2.2:14423069-14423088 MS.gene87270:CDS 55.0%
CATGTCGAGAGAAGTTGCGG+AGG - chr2.2:14424247-14424266 MS.gene87270:intron 55.0%
CCACCTCAACTCTCCAATGG+CGG - chr2.2:14423068-14423087 MS.gene87270:CDS 55.0%
CCATTGGAGAGTTGAGGTGG+GGG + chr2.2:14423068-14423087 None:intergenic 55.0%
CCCACCGAGTAAAAGAGGAG+AGG + chr2.2:14424320-14424339 None:intergenic 55.0%
CCCGCAACTGTGTCACCATT+CGG + chr2.2:14424470-14424489 None:intergenic 55.0%
CCGAATGGTGACACAGTTGC+GGG - chr2.2:14424467-14424486 MS.gene87270:intron 55.0%
CGAATGGTGACACAGTTGCG+GGG - chr2.2:14424468-14424487 MS.gene87270:intron 55.0%
CGACATGCCTTAGAGCTGGA+AGG + chr2.2:14424234-14424253 None:intergenic 55.0%
CGCCATTGGAGAGTTGAGGT+GGG + chr2.2:14423070-14423089 None:intergenic 55.0%
CTCTCGACATGCCTTAGAGC+TGG + chr2.2:14424238-14424257 None:intergenic 55.0%
GAAGGAGAAGTTGGAGCAGC+AGG - chr2.2:14424774-14424793 MS.gene87270:intron 55.0%
GAGAGAAGTTGCGGAGGGAT+AGG - chr2.2:14424253-14424272 MS.gene87270:intron 55.0%
GCAAATGCAGCAGGGATTGC+AGG + chr2.2:14424841-14424860 None:intergenic 55.0%
GCAGAGGCTTAAGGCTGAGA+AGG - chr2.2:14424756-14424775 MS.gene87270:intron 55.0%
GCCATTGGAGAGTTGAGGTG+GGG + chr2.2:14423069-14423088 None:intergenic 55.0%
TACCCGCCATTGGAGAGTTG+AGG + chr2.2:14423074-14423093 None:intergenic 55.0%
TTAGGCTCCATCTTGGAGCC+TGG - chr2.2:14424399-14424418 MS.gene87270:intron 55.0%
ATGGCTCCTTCGCCTTCACG+TGG - chr2.2:14423042-14423061 MS.gene87270:CDS 60.0%
CCCCCACCTCAACTCTCCAA+TGG - chr2.2:14423065-14423084 MS.gene87270:CDS 60.0%
CCGCCATTGGAGAGTTGAGG+TGG + chr2.2:14423071-14423090 None:intergenic 60.0%
CGATAGCAGGCTCCGTTCAG+TGG - chr2.2:14424097-14424116 MS.gene87270:intron 60.0%
GCTCCATCTTGGAGCCTGGA+AGG - chr2.2:14424403-14424422 MS.gene87270:intron 60.0%
TGGCCTTCCAGGCTCCAAGA+TGG + chr2.2:14424409-14424428 None:intergenic 60.0%
ATTCATGCCACCAGCCGCCG+TGG - chr2.2:14424927-14424946 MS.gene87270:CDS 65.0%
TTGAGGTGGGGGCCACGTGA+AGG + chr2.2:14423057-14423076 None:intergenic 65.0%
! TGTGAGGTATCCACGGCGGC+TGG + chr2.2:14424940-14424959 None:intergenic 65.0%
TGGGGGCCACGTGAAGGCGA+AGG + chr2.2:14423051-14423070 None:intergenic 70.0%
! GAGGTATCCACGGCGGCTGG+TGG + chr2.2:14424937-14424956 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 14422972 14424990 14422972 ID=MS.gene87270
chr2.2 mRNA 14422972 14424990 14422972 ID=MS.gene87270.t1;Parent=MS.gene87270
chr2.2 exon 14424872 14424990 14424872 ID=MS.gene87270.t1.exon1;Parent=MS.gene87270.t1
chr2.2 CDS 14424872 14424990 14424872 ID=cds.MS.gene87270.t1;Parent=MS.gene87270.t1
chr2.2 exon 14424044 14424109 14424044 ID=MS.gene87270.t1.exon2;Parent=MS.gene87270.t1
chr2.2 CDS 14424044 14424109 14424044 ID=cds.MS.gene87270.t1;Parent=MS.gene87270.t1
chr2.2 exon 14423676 14423753 14423676 ID=MS.gene87270.t1.exon3;Parent=MS.gene87270.t1
chr2.2 CDS 14423676 14423753 14423676 ID=cds.MS.gene87270.t1;Parent=MS.gene87270.t1
chr2.2 exon 14423411 14423573 14423411 ID=MS.gene87270.t1.exon4;Parent=MS.gene87270.t1
chr2.2 CDS 14423411 14423573 14423411 ID=cds.MS.gene87270.t1;Parent=MS.gene87270.t1
chr2.2 exon 14422972 14423235 14422972 ID=MS.gene87270.t1.exon5;Parent=MS.gene87270.t1
chr2.2 CDS 14422972 14423235 14422972 ID=cds.MS.gene87270.t1;Parent=MS.gene87270.t1
Gene Sequence

>MS.gene87270

ATGGTTTCCCCGGAAAACACCAATTGGCTTTTCGATTACCCTTTGATTGATGAAATTCCTGTTTCTGTCGATGGCTCCTTCGCCTTCACGTGGCCCCCACCTCAACTCTCCAATGGCGGGTATCTGATTTTGCCCCTTTTCTTAATTTAGGGTTTCAAGTGTTTGCCCCTTTTTTCAAATTTGTGGATAATTGTTGTGCTTTTTGTAAAGTTTGAATTTTTAAACTTGGGTTTGTAAAATTTTGTTGTAAAATTGTAGTTTTTATAAATTGTTGTGGTTTTCATTTGGTCTTCAGTTATTTGTTATGTTTTGTGTACTTATGGTTATGGTGGATTTGTAGTAGACAAGTGTAATTTTTGAATTGGTTAATGATGATGTTCTTGAATCTTCTGTTCTGGATACGTTGGTGGGTTTGATTGTCTGTGCTGCCTTGTTTCAATAGCTGTTGATGAAGCACCGACACATACACGAACACAAGACACGGTACTGACACTAGTAAACAATTTGAGAAAATTGAATAATTAAATGACACATGTGTGAGTGTTGTGTCGGTGTTGGGAATTGATATGCGTTGAACACTGGACACACTTTCAATTTGAAGTGTCAGTGCTACATAGATGCCTTAAGCTGCTTGTGCAAATTGACTACCTGCATCCCTTGTATTATGGAATACTGCGAACTAATGTGACTGATGCAATCGTAATTTAATTACAACAGCGATGATGTAGTTGTAGAGACCCCAATTACTGTTGCAATTCCTTTACCTTGGATTTTAGGTTTTGGTTTGAGAATTAGAATTTTTTTCTTCTATAAATACATATCTCCCATCCCATAGTATTAATTTACTAAGTTATGCTTCTGTGTTATCTCTCTGCTCCTAGTATTGAAATGGATGATAGCTCTCTAGTGGATTCTGATGGTATCAAAGAACCTGGTTCGAAGAAGAGGTAACTATTTTGTTTTACCTTTCATTTCTCATTAAACAAGTTAGTCAATGTTAATAAAATCAACACATGATCGACACGAGTAGTGTAGCGACTTAGCGTCAACTAACTTACAGCTTTGAGGTAATATAATTGAGTCAAATTGGCAATTTAACATGGTTTCTAAAGTGAAGATAATTAGCGATAGCAGGCTCCGTTCAGTGGCATCAATAAAAAAATTCCCTGTCAAATCCTATTGCTTGTATACTATAAGAAAAAGTTATCCCATATTTTTGTATTTTTTCTTGTTGCAGAGGTAGATCGGATTCATGTGCTCCTTCCAGCTCTAAGGCATGTCGAGAGAAGTTGCGGAGGGATAGGCTGAACGACAAGTAATCCCCACTATCTTGCCTTTTATTATTCCTCTCCTCTTTTACTCGGTGGGTGTAGACTGTGTATTTGGGGTATGCAACTAATATGATTGACATTGATAGGTTTGTTGAATTAGGCTCCATCTTGGAGCCTGGAAGGCCACCTAAAACAGACAAGGCAGCAATTCTGATTGATGCTGTCCGAATGGTGACACAGTTGCGGGGTGAAGCCCAAAAGTTGAAAGACTCAAATTCAGGTCTTCAAGAGAAGATTAAAGAGTTAAAGGTAAGGCCTCAATTGATTTAGAGCAATTTTCTTTGAGAGATAGAAGGTCCATATTCTAAATCATTTCATTTCCCTTCATTTTTATACTTTAACTGTAGTACTTTGTCATATCAGTGACTTCAATATCTGATTGACCTTATATAACACTGAATATTGAATTCTATCTACTATGCAGGTTGAGAAGAATGAACTTCGTGATGAGAAGCAGAGGCTTAAGGCTGAGAAGGAGAAGTTGGAGCAGCAGGTGAAATCAATGAACACCCAACCCAGTTTCTTGACACACCCTCCTGCAATCCCTGCTGCATTTGCTCATCAAGGCCAAGCCCCAAACAAGTTAATGCCTTTCATGAGTTATCCAGGAGTTGCCATGTGGCAATTCATGCCACCAGCCGCCGTGGATACCTCACAAGATCATGTACTCCGTCCACCAGTTGCCTAA
Protein sequence

>MS.gene87270.t1

MVSPENTNWLFDYPLIDEIPVSVDGSFAFTWPPPQLSNGGIEMDDSSLVDSDGIKEPGSKKRGRSDSCAPSSSKACREKLRRDRLNDKFVELGSILEPGRPPKTDKAAILIDAVRMVTQLRGEAQKLKDSNSGLQEKIKELKVEKNELRDEKQRLKAEKEKLEQQVKSMNTQPSFLTHPPAIPAAFAHQGQAPNKLMPFMSYPGVAMWQFMPPAAVDTSQDHVLRPPVA