Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87370.t1 | XP_013446432.1 | 98.9 | 88 | 1 | 0 | 1 | 88 | 1 | 88 | 2.10E-38 | 167.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87370.t1 | P0CB17 | 50.6 | 87 | 43 | 0 | 1 | 87 | 1 | 87 | 5.3e-19 | 94.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87370.t1 | G7ZVI6 | 98.9 | 88 | 1 | 0 | 1 | 88 | 1 | 88 | 1.5e-38 | 167.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050750 | MS.gene87370 | 0.809281 | 2.04E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene87370 | 0.802292 | 6.01E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene87370 | 0.816803 | 4.58E-52 | -1.69E-46 |
| MS.gene060322 | MS.gene87370 | 0.818705 | 1.70E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87370.t1 | MTR_8g078830 | 98.864 | 88 | 1 | 0 | 1 | 88 | 1 | 88 | 3.68e-58 | 175 |
| MS.gene87370.t1 | MTR_5g018680 | 67.045 | 88 | 29 | 0 | 1 | 88 | 1 | 88 | 1.03e-36 | 121 |
| MS.gene87370.t1 | MTR_4g068780 | 57.471 | 87 | 37 | 0 | 1 | 87 | 15 | 101 | 1.38e-29 | 103 |
| MS.gene87370.t1 | MTR_4g058950 | 37.931 | 87 | 54 | 0 | 1 | 87 | 35 | 121 | 1.81e-14 | 65.1 |
| MS.gene87370.t1 | MTR_1g073950 | 34.483 | 87 | 57 | 0 | 1 | 87 | 1 | 87 | 2.92e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87370.t1 | AT3G50810 | 50.575 | 87 | 43 | 0 | 1 | 87 | 1 | 87 | 8.06e-28 | 98.6 |
| MS.gene87370.t1 | AT3G50810 | 50.575 | 87 | 43 | 0 | 1 | 87 | 1 | 87 | 8.06e-28 | 98.6 |
| MS.gene87370.t1 | AT3G50810 | 50.575 | 87 | 43 | 0 | 1 | 87 | 1 | 87 | 8.06e-28 | 98.6 |
| MS.gene87370.t1 | AT4G37235 | 60.494 | 81 | 30 | 1 | 9 | 87 | 9 | 89 | 8.37e-27 | 96.3 |
| MS.gene87370.t1 | AT1G49405 | 43.678 | 87 | 49 | 0 | 1 | 87 | 1 | 87 | 1.46e-21 | 82.8 |
| MS.gene87370.t1 | AT5G02060 | 41.379 | 87 | 51 | 0 | 1 | 87 | 1 | 87 | 1.22e-12 | 59.7 |
Find 20 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTATGGTTACTCTGCTTTC+TGG | 0.095591 | 8.3:+25487872 | MS.gene87370:CDS |
| TACGATTACTTTCGGAGATA+TGG | 0.295674 | 8.3:+25489255 | MS.gene87370:CDS |
| TGCTTGGATGTTGATTTCTA+TGG | 0.343327 | 8.3:+25487856 | MS.gene87370:CDS |
| TGATGGGTTTGGTAATTTCC+TGG | 0.359500 | 8.3:+25489158 | MS.gene87370:CDS |
| GAGGAATTGCCAGGGGTGTT+AGG | 0.368859 | 8.3:+25487766 | MS.gene87370:CDS |
| TTCCTGGAGCACAACACTAT+TGG | 0.376363 | 8.3:+25489174 | MS.gene87370:CDS |
| TCCAGCTTTGCTCTTCGATT+AGG | 0.394428 | 8.3:+25487796 | MS.gene87370:CDS |
| TTTGGTAATGGTGATGGGTT+TGG | 0.404444 | 8.3:+25489147 | MS.gene87370:CDS |
| CACCAATAGTGTTGTGCTCC+AGG | 0.420340 | 8.3:-25489176 | None:intergenic |
| AAAATGAATCTCCGCTGCTT+CGG | 0.454686 | 8.3:-25489232 | None:intergenic |
| GCCTAATCGAAGAGCAAAGC+TGG | 0.459460 | 8.3:-25487797 | None:intergenic |
| GGTGGAATGGAGGAATTGCC+AGG | 0.464393 | 8.3:+25487757 | None:intergenic |
| CTGGAGCACAACACTATTGG+TGG | 0.509436 | 8.3:+25489177 | MS.gene87370:CDS |
| AAGCAGAGGAGAAGACAGCT+TGG | 0.543578 | 8.3:-25487819 | None:intergenic |
| GAGCTTGTTCCTAACACCCC+TGG | 0.567572 | 8.3:-25487775 | None:intergenic |
| CATGAAGAAAAGAGAAGCAG+AGG | 0.574247 | 8.3:-25487833 | None:intergenic |
| GAGCACAACACTATTGGTGG+TGG | 0.623288 | 8.3:+25489180 | MS.gene87370:CDS |
| TCAAAAGTGTACCGAAGCAG+CGG | 0.627998 | 8.3:+25489221 | MS.gene87370:CDS |
| GTGGAATGGAGGAATTGCCA+GGG | 0.633206 | 8.3:+25487758 | None:intergenic |
| TGGAATGGAGGAATTGCCAG+GGG | 0.755495 | 8.3:+25487759 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAAAGAAATAAAAATAGT+TGG | + | chr8.3:25488099-25488118 | MS.gene87370:intron | 15.0% |
| !! | CTAAAGAAATAAAAATAGTT+GGG | + | chr8.3:25488100-25488119 | MS.gene87370:intron | 15.0% |
| !! | TAAAGAAATAAAAATAGTTG+GGG | + | chr8.3:25488101-25488120 | MS.gene87370:intron | 15.0% |
| !! | AATACAAGTAAGAAAAAAAC+AGG | - | chr8.3:25488014-25488033 | None:intergenic | 20.0% |
| !! | ATACAAGTAAGAAAAAAACA+GGG | - | chr8.3:25488013-25488032 | None:intergenic | 20.0% |
| !! | TAATCTAAAAATGTCAGAAT+TGG | + | chr8.3:25489017-25489036 | MS.gene87370:intron | 20.0% |
| !! | TTGATTGAATTTGTATTGTT+TGG | - | chr8.3:25488918-25488937 | None:intergenic | 20.0% |
| !!! | AGTATTATATTTTGGAAGTT+AGG | + | chr8.3:25488169-25488188 | MS.gene87370:intron | 20.0% |
| !!! | ATTCTGACATTTTTAGATTA+AGG | - | chr8.3:25489017-25489036 | None:intergenic | 20.0% |
| !!! | TGTTTCAACTTTTTTTTCAA+AGG | + | chr8.3:25488218-25488237 | MS.gene87370:intron | 20.0% |
| !!! | TTAAAACATTTTGGCATTAT+CGG | + | chr8.3:25488646-25488665 | MS.gene87370:intron | 20.0% |
| !!! | TTCATTTTTACGATTACTTT+CGG | + | chr8.3:25489247-25489266 | MS.gene87370:CDS | 20.0% |
| ! | AAAATGTCAGAATTGGTAAA+AGG | + | chr8.3:25489024-25489043 | MS.gene87370:intron | 25.0% |
| ! | ATGTACAATGGTCTAATTAT+AGG | + | chr8.3:25488400-25488419 | MS.gene87370:intron | 25.0% |
| ! | TACAAGTAAGAAAAAAACAG+GGG | - | chr8.3:25488012-25488031 | None:intergenic | 25.0% |
| ! | TGTCTTAGTTAAGCTAATTA+GGG | + | chr8.3:25488140-25488159 | MS.gene87370:intron | 25.0% |
| ! | TTGTCTTAGTTAAGCTAATT+AGG | + | chr8.3:25488139-25488158 | MS.gene87370:intron | 25.0% |
| !! | CAGAGAAAGTTAAAACATTT+TGG | + | chr8.3:25488637-25488656 | MS.gene87370:intron | 25.0% |
| !! | TTTTCTTACTTGTATTTGCA+TGG | + | chr8.3:25488018-25488037 | MS.gene87370:intron | 25.0% |
| !!! | ATAAGGTTTTGATTTTTGGT+TGG | + | chr8.3:25488512-25488531 | MS.gene87370:intron | 25.0% |
| !!! | CAAAATGTTTTAACTTTCTC+TGG | - | chr8.3:25488639-25488658 | None:intergenic | 25.0% |
| !!! | CATGTTTTTTTCTTTCTTCT+GGG | + | chr8.3:25487928-25487947 | MS.gene87370:intron | 25.0% |
| !!! | CTTCTATGTCATTTTAAGAA+GGG | + | chr8.3:25488462-25488481 | MS.gene87370:intron | 25.0% |
| !!! | GGGGTAAAAGTATTATATTT+TGG | + | chr8.3:25488161-25488180 | MS.gene87370:intron | 25.0% |
| !!! | TAAGGTTTTGATTTTTGGTT+GGG | + | chr8.3:25488513-25488532 | MS.gene87370:intron | 25.0% |
| !!! | TCATGTTTTTTTCTTTCTTC+TGG | + | chr8.3:25487927-25487946 | MS.gene87370:intron | 25.0% |
| !!! | TGTCATAAGGTTTTGATTTT+TGG | + | chr8.3:25488508-25488527 | MS.gene87370:intron | 25.0% |
| !!! | TTATGAGTTCTGAATATGTT+TGG | + | chr8.3:25488055-25488074 | MS.gene87370:intron | 25.0% |
| AGAGAATTACGTTGAATTAG+CGG | + | chr8.3:25488573-25488592 | MS.gene87370:intron | 30.0% | |
| GTCTTAGTTAAGCTAATTAG+GGG | + | chr8.3:25488141-25488160 | MS.gene87370:intron | 30.0% | |
| TCTTAGTTAAGCTAATTAGG+GGG | + | chr8.3:25488142-25488161 | MS.gene87370:intron | 30.0% | |
| TGTCCAACATTGAAATATGT+CGG | + | chr8.3:25488367-25488386 | MS.gene87370:intron | 30.0% | |
| TTAACTTTCTCTGGTGTTTA+TGG | - | chr8.3:25488630-25488649 | None:intergenic | 30.0% | |
| TTATAGCTGATAGCTTAGAA+CGG | - | chr8.3:25488950-25488969 | None:intergenic | 30.0% | |
| TTCAGAACTCATAAAATCAC+TGG | - | chr8.3:25488049-25488068 | None:intergenic | 30.0% | |
| ! | CATTGATTAGCAATGTCATA+AGG | + | chr8.3:25488495-25488514 | MS.gene87370:intron | 30.0% |
| ! | TCAGAATTGGTAAAAGGTTT+TGG | + | chr8.3:25489030-25489049 | MS.gene87370:intron | 30.0% |
| !!! | AAGGTTTTGATTTTTGGTTG+GGG | + | chr8.3:25488514-25488533 | MS.gene87370:intron | 30.0% |
| !!! | GCTTCTATGTCATTTTAAGA+AGG | + | chr8.3:25488461-25488480 | MS.gene87370:intron | 30.0% |
| AGACCATTGTACATCAATGT+AGG | - | chr8.3:25488394-25488413 | None:intergenic | 35.0% | |
| ATGAAGCACAGACACATATA+CGG | + | chr8.3:25488259-25488278 | MS.gene87370:intron | 35.0% | |
| CTTACTTGTATTTGCATGGT+GGG | + | chr8.3:25488022-25488041 | MS.gene87370:intron | 35.0% | |
| TAAGATTGCAAAAACAGTGC+TGG | - | chr8.3:25488743-25488762 | None:intergenic | 35.0% | |
| TCATAATCACCATGTTGAAG+TGG | - | chr8.3:25488818-25488837 | None:intergenic | 35.0% | |
| TCTTACTTGTATTTGCATGG+TGG | + | chr8.3:25488021-25488040 | MS.gene87370:intron | 35.0% | |
| ! | TCTGTTATTGCACAGCTTTT+TGG | + | chr8.3:25489129-25489148 | MS.gene87370:intron | 35.0% |
| ! | TTCTCTTTTCTTCATGTGCT+TGG | + | chr8.3:25487840-25487859 | MS.gene87370:CDS | 35.0% |
| !! | ATTGCACAGCTTTTTGGTAA+TGG | + | chr8.3:25489135-25489154 | MS.gene87370:intron | 35.0% |
| !! | TGCTTGGATGTTGATTTCTA+TGG | + | chr8.3:25487856-25487875 | MS.gene87370:CDS | 35.0% |
| !!! | AGCTTTTTGGTAATGGTGAT+GGG | + | chr8.3:25489142-25489161 | MS.gene87370:intron | 35.0% |
| AAAATGAATCTCCGCTGCTT+CGG | - | chr8.3:25489235-25489254 | None:intergenic | 40.0% | |
| CATGAAGAAAAGAGAAGCAG+AGG | - | chr8.3:25487836-25487855 | None:intergenic | 40.0% | |
| GGACCTACATTGATGTACAA+TGG | + | chr8.3:25488388-25488407 | MS.gene87370:intron | 40.0% | |
| GGTCCGACATATTTCAATGT+TGG | - | chr8.3:25488373-25488392 | None:intergenic | 40.0% | |
| TCTATGGTTACTCTGCTTTC+TGG | + | chr8.3:25487872-25487891 | MS.gene87370:CDS | 40.0% | |
| TTAGTGCAATCTCACTACCA+AGG | + | chr8.3:25488792-25488811 | MS.gene87370:intron | 40.0% | |
| TTCAATGTTGGACACTGACA+CGG | - | chr8.3:25488361-25488380 | None:intergenic | 40.0% | |
| ! | TGGTAGTGAGATTGCACTAA+GGG | - | chr8.3:25488792-25488811 | None:intergenic | 40.0% |
| !! | ACATTTTGGCATTATCGGAG+AGG | + | chr8.3:25488651-25488670 | MS.gene87370:intron | 40.0% |
| !! | TGATGGGTTTGGTAATTTCC+TGG | + | chr8.3:25489158-25489177 | MS.gene87370:CDS | 40.0% |
| !! | TTAGTTTGTTGCCAAGTAGC+CGG | + | chr8.3:25488604-25488623 | MS.gene87370:intron | 40.0% |
| !! | TTGGTAGTGAGATTGCACTA+AGG | - | chr8.3:25488793-25488812 | None:intergenic | 40.0% |
| !! | TTTGGTAATGGTGATGGGTT+TGG | + | chr8.3:25489147-25489166 | MS.gene87370:CDS | 40.0% |
| !!! | CAGCTTTTTGGTAATGGTGA+TGG | + | chr8.3:25489141-25489160 | MS.gene87370:intron | 40.0% |
| CTGTGCTTCATAGCGAGTAT+TGG | - | chr8.3:25488250-25488269 | None:intergenic | 45.0% | |
| CTTTCTCTGGTGTTTATGGC+CGG | - | chr8.3:25488626-25488645 | None:intergenic | 45.0% | |
| TCCAGCTTTGCTCTTCGATT+AGG | + | chr8.3:25487796-25487815 | MS.gene87370:CDS | 45.0% | |
| TGCTTCAGAAGATGCTGCAA+TGG | + | chr8.3:25489070-25489089 | MS.gene87370:intron | 45.0% | |
| TTCCTGGAGCACAACACTAT+TGG | + | chr8.3:25489174-25489193 | MS.gene87370:CDS | 45.0% | |
| ! | TCAAAAGTGTACCGAAGCAG+CGG | + | chr8.3:25489221-25489240 | MS.gene87370:CDS | 45.0% |
| !! | ATTGCAGCATCTTCTGAAGC+AGG | - | chr8.3:25489071-25489090 | None:intergenic | 45.0% |
| AAGCAGAGGAGAAGACAGCT+TGG | - | chr8.3:25487822-25487841 | None:intergenic | 50.0% | |
| ATGCTGCAATGGCAATGTCG+CGG | + | chr8.3:25489081-25489100 | MS.gene87370:intron | 50.0% | |
| CACCAATAGTGTTGTGCTCC+AGG | - | chr8.3:25489179-25489198 | None:intergenic | 50.0% | |
| CTACCAAGGCCACTTCAACA+TGG | + | chr8.3:25488806-25488825 | MS.gene87370:intron | 50.0% | |
| CTGGAGCACAACACTATTGG+TGG | + | chr8.3:25489177-25489196 | MS.gene87370:CDS | 50.0% | |
| GAGCACAACACTATTGGTGG+TGG | + | chr8.3:25489180-25489199 | MS.gene87370:CDS | 50.0% | |
| GCCTAATCGAAGAGCAAAGC+TGG | - | chr8.3:25487800-25487819 | None:intergenic | 50.0% | |
| TCACCATGTTGAAGTGGCCT+TGG | - | chr8.3:25488812-25488831 | None:intergenic | 50.0% | |
| GGTGTTTATGGCCGGCTACT+TGG | - | chr8.3:25488618-25488637 | None:intergenic | 55.0% | |
| ! | GAGCTTGTTCCTAACACCCC+TGG | - | chr8.3:25487778-25487797 | None:intergenic | 55.0% |
| !! | GAGGAATTGCCAGGGGTGTT+AGG | + | chr8.3:25487766-25487785 | MS.gene87370:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 25487763 | 25489276 | 25487763 | ID=MS.gene87370 |
| chr8.3 | mRNA | 25487763 | 25489276 | 25487763 | ID=MS.gene87370.t1;Parent=MS.gene87370 |
| chr8.3 | exon | 25487763 | 25487893 | 25487763 | ID=MS.gene87370.t1.exon1;Parent=MS.gene87370.t1 |
| chr8.3 | CDS | 25487763 | 25487893 | 25487763 | ID=cds.MS.gene87370.t1;Parent=MS.gene87370.t1 |
| chr8.3 | exon | 25489144 | 25489276 | 25489144 | ID=MS.gene87370.t1.exon2;Parent=MS.gene87370.t1 |
| chr8.3 | CDS | 25489144 | 25489276 | 25489144 | ID=cds.MS.gene87370.t1;Parent=MS.gene87370.t1 |
| Gene Sequence |
| Protein sequence |
