Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87489.t1 | KEH29073.1 | 94.2 | 138 | 6 | 1 | 1 | 138 | 1 | 136 | 1.30E-47 | 199.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87489.t1 | A0A072UIZ3 | 94.2 | 138 | 6 | 1 | 1 | 138 | 1 | 136 | 9.4e-48 | 199.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051719 | MS.gene87489 | 0.825185 | 5.38E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene87489 | 0.83573 | 1.42E-56 | -1.69E-46 |
| MS.gene055466 | MS.gene87489 | 0.802196 | 6.29E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene87489 | 0.801943 | 7.09E-49 | -1.69E-46 |
| MS.gene058339 | MS.gene87489 | 0.809639 | 1.71E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87489.t1 | MTR_4g021870 | 92.029 | 138 | 9 | 1 | 1 | 138 | 1 | 136 | 1.46e-82 | 238 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAATAATGTTGATGTTTA+AGG | 0.130565 | 4.1:-76140694 | MS.gene87489:CDS |
| TCCTTAAACATCAACATTAT+TGG | 0.190065 | 4.1:+76140693 | None:intergenic |
| CTATTCTCTTCGAGCTTTCT+TGG | 0.218688 | 4.1:+76140377 | None:intergenic |
| GTGGACATTGCTTTGGCATT+TGG | 0.289133 | 4.1:+76140532 | None:intergenic |
| GGTGCAAGTGGACATTGCTT+TGG | 0.418817 | 4.1:+76140525 | None:intergenic |
| ATTCTATTATCAAAATGAGT+TGG | 0.466175 | 4.1:+76140555 | None:intergenic |
| CCAAACCACATATGAAAAGA+AGG | 0.494232 | 4.1:-76140494 | MS.gene87489:CDS |
| AACAACATCATCAAGAGAGT+TGG | 0.513276 | 4.1:-76140619 | MS.gene87489:CDS |
| ACTACTTGATGTGTCAAGAG+AGG | 0.671924 | 4.1:-76140454 | MS.gene87489:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCTATTATCAAAATGAGT+TGG | + | chr4.1:76140596-76140615 | None:intergenic | 20.0% |
| !!! | AAAATGTTTTGTTCTTGATT+TGG | + | chr4.1:76140733-76140752 | None:intergenic | 20.0% |
| ! | ACCAATAATGTTGATGTTTA+AGG | - | chr4.1:76140454-76140473 | MS.gene87489:CDS | 25.0% |
| ! | TAAGCAAAAACAAGATTTAG+AGG | - | chr4.1:76140499-76140518 | MS.gene87489:CDS | 25.0% |
| ! | TCCTTAAACATCAACATTAT+TGG | + | chr4.1:76140458-76140477 | None:intergenic | 25.0% |
| GAAGAAAAAGATGAAGATGA+TGG | - | chr4.1:76140560-76140579 | MS.gene87489:CDS | 30.0% | |
| !!! | ATATGTGGTTTGGTTTTTCT+TGG | + | chr4.1:76140647-76140666 | None:intergenic | 30.0% |
| AACAACATCATCAAGAGAGT+TGG | - | chr4.1:76140529-76140548 | MS.gene87489:CDS | 35.0% | |
| CCAAACCACATATGAAAAGA+AGG | - | chr4.1:76140654-76140673 | MS.gene87489:CDS | 35.0% | |
| ! | CCTTCTTTTCATATGTGGTT+TGG | + | chr4.1:76140657-76140676 | None:intergenic | 35.0% |
| ! | GCTTTCCTTCTTTTCATATG+TGG | + | chr4.1:76140662-76140681 | None:intergenic | 35.0% |
| ACTACTTGATGTGTCAAGAG+AGG | - | chr4.1:76140694-76140713 | MS.gene87489:CDS | 40.0% | |
| CTATTCTCTTCGAGCTTTCT+TGG | + | chr4.1:76140774-76140793 | None:intergenic | 40.0% | |
| !! | TTGGTTTTTCTTGGTGCAAG+TGG | + | chr4.1:76140638-76140657 | None:intergenic | 40.0% |
| GTGGACATTGCTTTGGCATT+TGG | + | chr4.1:76140619-76140638 | None:intergenic | 45.0% | |
| GGTGCAAGTGGACATTGCTT+TGG | + | chr4.1:76140626-76140645 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 76140377 | 76140793 | 76140377 | ID=MS.gene87489 |
| chr4.1 | mRNA | 76140377 | 76140793 | 76140377 | ID=MS.gene87489.t1;Parent=MS.gene87489 |
| chr4.1 | exon | 76140377 | 76140793 | 76140377 | ID=MS.gene87489.t1.exon1;Parent=MS.gene87489.t1 |
| chr4.1 | CDS | 76140377 | 76140793 | 76140377 | ID=cds.MS.gene87489.t1;Parent=MS.gene87489.t1 |
| Gene Sequence |
| Protein sequence |
