Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87658.t1 | XP_003601009.1 | 97.4 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 1.60E-54 | 221.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87658.t1 | G7J2F5 | 97.4 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 1.1e-54 | 221.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052176 | MS.gene87658 | 0.806046 | 9.95E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87658.t1 | MTR_3g072010 | 97.391 | 115 | 3 | 0 | 1 | 115 | 1 | 115 | 3.12e-80 | 232 |
| MS.gene87658.t1 | MTR_7g071210 | 68.000 | 50 | 16 | 0 | 66 | 115 | 12 | 61 | 4.81e-21 | 80.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87658.t1 | AT5G05360 | 39.474 | 114 | 51 | 4 | 16 | 115 | 17 | 126 | 5.34e-21 | 82.8 |
| MS.gene87658.t1 | AT5G05360 | 39.474 | 114 | 51 | 4 | 16 | 115 | 17 | 126 | 5.75e-21 | 82.4 |
| MS.gene87658.t1 | AT2G38450 | 59.184 | 49 | 20 | 0 | 67 | 115 | 55 | 103 | 2.01e-16 | 70.1 |
| MS.gene87658.t1 | AT2G38450 | 59.184 | 49 | 20 | 0 | 67 | 115 | 55 | 103 | 3.57e-16 | 69.7 |
Find 35 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGCGGTCTTGTTGTTTCT+TGG | 0.311175 | 3.1:+58834102 | None:intergenic |
| GATACGGGTTGGGTTTGTTT+TGG | 0.339121 | 3.1:+58834154 | None:intergenic |
| CAAATGTATTGCTCTGGATA+TGG | 0.370151 | 3.1:-58833926 | MS.gene87658:CDS |
| TCCACAGGCTCCACCTTCTT+CGG | 0.388108 | 3.1:+58834058 | None:intergenic |
| GTGGTTTCACGATACGCTTA+AGG | 0.417498 | 3.1:-58833990 | MS.gene87658:CDS |
| ACTTGAAAGATCCCCACGTT+TGG | 0.417801 | 3.1:+58833964 | None:intergenic |
| CTGGTTCAATGATACGGGTT+GGG | 0.430102 | 3.1:+58834144 | None:intergenic |
| CTTTCAAGTCAAGTTCTTGT+TGG | 0.438409 | 3.1:-58833950 | MS.gene87658:CDS |
| TGGTTCGTGCAGCGATGAAC+TGG | 0.442996 | 3.1:+58834125 | None:intergenic |
| ACAAGACCGCACTGTTGTTA+AGG | 0.443164 | 3.1:-58834092 | MS.gene87658:CDS |
| AAACTCGAATAGCTCTTTCC+AGG | 0.469121 | 3.1:-58834036 | MS.gene87658:CDS |
| GTTGGTCAAATGTATTGCTC+TGG | 0.481330 | 3.1:-58833932 | MS.gene87658:CDS |
| GATGAACTGGTTCAATGATA+CGG | 0.513998 | 3.1:+58834138 | None:intergenic |
| GCGGTCTTGTTGTTTCTTGG+TGG | 0.525060 | 3.1:+58834105 | None:intergenic |
| GGTGGAATCGCCGCCGAAGA+AGG | 0.532810 | 3.1:-58834071 | MS.gene87658:CDS |
| TTCTTGTCGCACAGAGTCAA+AGG | 0.533961 | 3.1:+58834216 | None:intergenic |
| AAACCACCGATGAGTGCAAT+CGG | 0.533991 | 3.1:+58834006 | None:intergenic |
| ATGAACTGGTTCAATGATAC+GGG | 0.546395 | 3.1:+58834139 | None:intergenic |
| CGCTTAAGGAGGCCAAACGT+GGG | 0.554828 | 3.1:-58833976 | MS.gene87658:CDS |
| TGTTTCTAGAAATCCTCAAA+AGG | 0.559783 | 3.1:-58833903 | None:intergenic |
| ACGCTTAAGGAGGCCAAACG+TGG | 0.562570 | 3.1:-58833977 | MS.gene87658:CDS |
| ACTGGTTCAATGATACGGGT+TGG | 0.564172 | 3.1:+58834143 | None:intergenic |
| TGGTTGCCGATTGCACTCAT+CGG | 0.567470 | 3.1:-58834012 | MS.gene87658:CDS |
| GCCGAAGAAGGTGGAGCCTG+TGG | 0.572829 | 3.1:-58834059 | MS.gene87658:CDS |
| AGAGCTATTCGAGTTTCCAC+AGG | 0.573631 | 3.1:+58834043 | None:intergenic |
| AGTGCAATCGGCAACCACCC+TGG | 0.583008 | 3.1:+58834018 | None:intergenic |
| GGAATCGCCGCCGAAGAAGG+TGG | 0.584881 | 3.1:-58834068 | MS.gene87658:CDS |
| TTGCCGATTGCACTCATCGG+TGG | 0.591814 | 3.1:-58834009 | MS.gene87658:CDS |
| AACTCGAATAGCTCTTTCCA+GGG | 0.616905 | 3.1:-58834035 | MS.gene87658:CDS |
| GTTTCACGATACGCTTAAGG+AGG | 0.639685 | 3.1:-58833987 | MS.gene87658:CDS |
| AGACCGCACTGTTGTTAAGG+TGG | 0.642343 | 3.1:-58834089 | MS.gene87658:CDS |
| ACAGGCTCCACCTTCTTCGG+CGG | 0.656515 | 3.1:+58834061 | None:intergenic |
| GCTTAAGGAGGCCAAACGTG+GGG | 0.679928 | 3.1:-58833975 | MS.gene87658:CDS |
| TCGAATAGCTCTTTCCAGGG+TGG | 0.692357 | 3.1:-58834032 | MS.gene87658:CDS |
| ATTCCACCTTAACAACAGTG+CGG | 0.796242 | 3.1:+58834086 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAACTGGTTCAATGATAC+GGG | + | chr3.1:58833994-58834013 | None:intergenic | 35.0% |
| ! | CAAATGTATTGCTCTGGATA+TGG | - | chr3.1:58834204-58834223 | MS.gene87658:CDS | 35.0% |
| ! | CTTTCAAGTCAAGTTCTTGT+TGG | - | chr3.1:58834180-58834199 | MS.gene87658:CDS | 35.0% |
| ! | GATGAACTGGTTCAATGATA+CGG | + | chr3.1:58833995-58834014 | None:intergenic | 35.0% |
| AAACTCGAATAGCTCTTTCC+AGG | - | chr3.1:58834094-58834113 | MS.gene87658:CDS | 40.0% | |
| AACTCGAATAGCTCTTTCCA+GGG | - | chr3.1:58834095-58834114 | MS.gene87658:CDS | 40.0% | |
| ATTCCACCTTAACAACAGTG+CGG | + | chr3.1:58834047-58834066 | None:intergenic | 40.0% | |
| GTTGGTCAAATGTATTGCTC+TGG | - | chr3.1:58834198-58834217 | MS.gene87658:CDS | 40.0% | |
| !!! | GTTGGGTTTGTTTTGGTCTA+TGG | + | chr3.1:58833972-58833991 | None:intergenic | 40.0% |
| AAACCACCGATGAGTGCAAT+CGG | + | chr3.1:58834127-58834146 | None:intergenic | 45.0% | |
| ACAAGACCGCACTGTTGTTA+AGG | - | chr3.1:58834038-58834057 | MS.gene87658:CDS | 45.0% | |
| ACTTGAAAGATCCCCACGTT+TGG | + | chr3.1:58834169-58834188 | None:intergenic | 45.0% | |
| CTGGTTCAATGATACGGGTT+GGG | + | chr3.1:58833989-58834008 | None:intergenic | 45.0% | |
| GTGGTTTCACGATACGCTTA+AGG | - | chr3.1:58834140-58834159 | MS.gene87658:CDS | 45.0% | |
| GTTTCACGATACGCTTAAGG+AGG | - | chr3.1:58834143-58834162 | MS.gene87658:CDS | 45.0% | |
| TTCTTGTCGCACAGAGTCAA+AGG | + | chr3.1:58833917-58833936 | None:intergenic | 45.0% | |
| ! | ACTGGTTCAATGATACGGGT+TGG | + | chr3.1:58833990-58834009 | None:intergenic | 45.0% |
| ! | AGAGCTATTCGAGTTTCCAC+AGG | + | chr3.1:58834090-58834109 | None:intergenic | 45.0% |
| !! | AGTGCGGTCTTGTTGTTTCT+TGG | + | chr3.1:58834031-58834050 | None:intergenic | 45.0% |
| !!! | GATACGGGTTGGGTTTGTTT+TGG | + | chr3.1:58833979-58833998 | None:intergenic | 45.0% |
| AGACCGCACTGTTGTTAAGG+TGG | - | chr3.1:58834041-58834060 | MS.gene87658:CDS | 50.0% | |
| TCGAATAGCTCTTTCCAGGG+TGG | - | chr3.1:58834098-58834117 | MS.gene87658:CDS | 50.0% | |
| ! | TGGTTGCCGATTGCACTCAT+CGG | - | chr3.1:58834118-58834137 | MS.gene87658:CDS | 50.0% |
| !! | GCGGTCTTGTTGTTTCTTGG+TGG | + | chr3.1:58834028-58834047 | None:intergenic | 50.0% |
| ACGCTTAAGGAGGCCAAACG+TGG | - | chr3.1:58834153-58834172 | MS.gene87658:CDS | 55.0% | |
| CGCTTAAGGAGGCCAAACGT+GGG | - | chr3.1:58834154-58834173 | MS.gene87658:CDS | 55.0% | |
| GCTTAAGGAGGCCAAACGTG+GGG | - | chr3.1:58834155-58834174 | MS.gene87658:CDS | 55.0% | |
| TCCACAGGCTCCACCTTCTT+CGG | + | chr3.1:58834075-58834094 | None:intergenic | 55.0% | |
| TGGTTCGTGCAGCGATGAAC+TGG | + | chr3.1:58834008-58834027 | None:intergenic | 55.0% | |
| ! | TTGCCGATTGCACTCATCGG+TGG | - | chr3.1:58834121-58834140 | MS.gene87658:CDS | 55.0% |
| ACAGGCTCCACCTTCTTCGG+CGG | + | chr3.1:58834072-58834091 | None:intergenic | 60.0% | |
| AGTGCAATCGGCAACCACCC+TGG | + | chr3.1:58834115-58834134 | None:intergenic | 60.0% | |
| GCCGAAGAAGGTGGAGCCTG+TGG | - | chr3.1:58834071-58834090 | MS.gene87658:CDS | 65.0% | |
| GGAATCGCCGCCGAAGAAGG+TGG | - | chr3.1:58834062-58834081 | MS.gene87658:CDS | 65.0% | |
| GGTGGAATCGCCGCCGAAGA+AGG | - | chr3.1:58834059-58834078 | MS.gene87658:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 58833904 | 58834248 | 58833904 | ID=MS.gene87658 |
| chr3.1 | mRNA | 58833904 | 58834248 | 58833904 | ID=MS.gene87658.t1;Parent=MS.gene87658 |
| chr3.1 | exon | 58833904 | 58834248 | 58833904 | ID=MS.gene87658.t1.exon1;Parent=MS.gene87658.t1 |
| chr3.1 | CDS | 58833904 | 58834248 | 58833904 | ID=cds.MS.gene87658.t1;Parent=MS.gene87658.t1 |
| Gene Sequence |
| Protein sequence |
