Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87690.t1 | XP_013449250.1 | 91.7 | 168 | 11 | 1 | 1 | 165 | 1 | 168 | 1.10E-72 | 282.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87690.t1 | A0A072U0R7 | 91.7 | 168 | 11 | 1 | 1 | 165 | 1 | 168 | 7.8e-73 | 282.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049989 | MS.gene87690 | 0.813851 | 2.08E-51 | -1.69E-46 |
| MS.gene054614 | MS.gene87690 | 0.801723 | 7.87E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87690.t1 | MTR_7g073500 | 91.667 | 168 | 11 | 1 | 1 | 165 | 1 | 168 | 3.65e-105 | 300 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87690.t1 | AT5G01910 | 49.635 | 137 | 54 | 3 | 19 | 152 | 4 | 128 | 4.46e-30 | 107 |
| MS.gene87690.t1 | AT5G01910 | 49.635 | 137 | 54 | 3 | 19 | 152 | 4 | 128 | 4.78e-30 | 108 |
Find 30 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGATCGGCGGGAGTGTTTA+TGG | 0.245932 | 7.1:-28891212 | None:intergenic |
| CGATAGATTGTTCCGTTTGA+AGG | 0.364571 | 7.1:+28891507 | MS.gene87690:CDS |
| GAAACATAATTGATCGAGCT+TGG | 0.412716 | 7.1:-28891263 | None:intergenic |
| GAGCTTGGGTTTGTGCTTTG+TGG | 0.423141 | 7.1:-28891248 | None:intergenic |
| GTGTCCGGCCAGAGCGTGGC+CGG | 0.445137 | 7.1:-28891180 | None:intergenic |
| AAACATAATTGATCGAGCTT+GGG | 0.455044 | 7.1:-28891262 | None:intergenic |
| AGATCGGCGGGAGTGTTTAT+GGG | 0.461473 | 7.1:-28891211 | None:intergenic |
| TATTCGAACCCTTAGAGAAA+AGG | 0.487625 | 7.1:+28891650 | MS.gene87690:CDS |
| GTTGCTTGACGCGAAGATGA+AGG | 0.492065 | 7.1:+28891438 | MS.gene87690:CDS |
| TCCGATCACCACTTCTCCAC+TGG | 0.492835 | 7.1:+28891315 | MS.gene87690:CDS |
| GTTTGTGCTTTGTGGAAAGA+TGG | 0.506717 | 7.1:-28891240 | None:intergenic |
| AGATGGTGAGTGAGATCGGC+GGG | 0.512562 | 7.1:-28891223 | None:intergenic |
| GATATTTGAGTTCCTTCAAA+CGG | 0.520675 | 7.1:-28891519 | None:intergenic |
| GACTGCGATGAAGAAGAAGC+TGG | 0.522184 | 7.1:+28891360 | MS.gene87690:CDS |
| AAATCCGGCCACGCTCTGGC+CGG | 0.529930 | 7.1:+28891176 | MS.gene87690:CDS |
| TTATGGGCTGAAGTGGTGTC+CGG | 0.550025 | 7.1:-28891195 | None:intergenic |
| TGACGCGAAGATGAAGGAGA+TGG | 0.559497 | 7.1:+28891444 | MS.gene87690:CDS |
| AGTGGTGTCCGGCCAGAGCG+TGG | 0.560975 | 7.1:-28891184 | None:intergenic |
| CGACGTCTCCAGTGGAGAAG+TGG | 0.569616 | 7.1:-28891323 | None:intergenic |
| ACTGGAGACGTCGCTTGCAG+AGG | 0.576756 | 7.1:+28891333 | MS.gene87690:CDS |
| GGAGTGTTTATGGGCTGAAG+TGG | 0.581810 | 7.1:-28891202 | None:intergenic |
| GGAAAGATGGTGAGTGAGAT+CGG | 0.586737 | 7.1:-28891227 | None:intergenic |
| TCCAGTGGAGAAGTGGTGAT+CGG | 0.588457 | 7.1:-28891316 | None:intergenic |
| TCTGCAAGCGACGTCTCCAG+TGG | 0.592842 | 7.1:-28891331 | None:intergenic |
| CTTATGCAAACACCCGCCAC+CGG | 0.606622 | 7.1:+28891145 | MS.gene87690:CDS |
| TTTCATACCTCTGATGTCGA+TGG | 0.613539 | 7.1:-28891702 | None:intergenic |
| CGAAATCACACCGATCCAGA+AGG | 0.617005 | 7.1:+28891285 | MS.gene87690:CDS |
| GGAACTCAAATATCGATGCA+TGG | 0.636808 | 7.1:+28891528 | MS.gene87690:CDS |
| AACAGCACCATCGACATCAG+AGG | 0.666142 | 7.1:+28891695 | MS.gene87690:CDS |
| AAGATGGTGAGTGAGATCGG+CGG | 0.726270 | 7.1:-28891224 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACATAATTGATCGAGCTT+GGG | - | chr7.1:28891265-28891284 | None:intergenic | 30.0% | |
| AGAAACAAAACGAAACTGAA+TGG | - | chr7.1:28891587-28891606 | None:intergenic | 30.0% | |
| ! | GATATTTGAGTTCCTTCAAA+CGG | - | chr7.1:28891522-28891541 | None:intergenic | 30.0% |
| ! | TTCATGTTCCTTTTCTCTAA+GGG | - | chr7.1:28891661-28891680 | None:intergenic | 30.0% |
| ! | TTTCATGTTCCTTTTCTCTA+AGG | - | chr7.1:28891662-28891681 | None:intergenic | 30.0% |
| GAAACATAATTGATCGAGCT+TGG | - | chr7.1:28891266-28891285 | None:intergenic | 35.0% | |
| TATTCGAACCCTTAGAGAAA+AGG | + | chr7.1:28891650-28891669 | MS.gene87690:CDS | 35.0% | |
| !! | TTTTCTCTAAGGGTTCGAAT+AGG | - | chr7.1:28891651-28891670 | None:intergenic | 35.0% |
| CGATAGATTGTTCCGTTTGA+AGG | + | chr7.1:28891507-28891526 | MS.gene87690:CDS | 40.0% | |
| GGAACTCAAATATCGATGCA+TGG | + | chr7.1:28891528-28891547 | MS.gene87690:CDS | 40.0% | |
| GTTTGTGCTTTGTGGAAAGA+TGG | - | chr7.1:28891243-28891262 | None:intergenic | 40.0% | |
| GGAAAGATGGTGAGTGAGAT+CGG | - | chr7.1:28891230-28891249 | None:intergenic | 45.0% | |
| ! | GGACAGTTTTCCTTCTGGAT+CGG | - | chr7.1:28891298-28891317 | None:intergenic | 45.0% |
| ! | TGATCGGACAGTTTTCCTTC+TGG | - | chr7.1:28891303-28891322 | None:intergenic | 45.0% |
| AACAGCACCATCGACATCAG+AGG | + | chr7.1:28891695-28891714 | MS.gene87690:CDS | 50.0% | |
| AAGATGGTGAGTGAGATCGG+CGG | - | chr7.1:28891227-28891246 | None:intergenic | 50.0% | |
| CCACCGGAACACCAAAAATC+CGG | + | chr7.1:28891161-28891180 | MS.gene87690:CDS | 50.0% | |
| CGAAATCACACCGATCCAGA+AGG | + | chr7.1:28891285-28891304 | MS.gene87690:CDS | 50.0% | |
| GACTGCGATGAAGAAGAAGC+TGG | + | chr7.1:28891360-28891379 | MS.gene87690:CDS | 50.0% | |
| GAGCTTGGGTTTGTGCTTTG+TGG | - | chr7.1:28891251-28891270 | None:intergenic | 50.0% | |
| GTTGCTTGACGCGAAGATGA+AGG | + | chr7.1:28891438-28891457 | MS.gene87690:CDS | 50.0% | |
| TCCAGTGGAGAAGTGGTGAT+CGG | - | chr7.1:28891319-28891338 | None:intergenic | 50.0% | |
| TGACGCGAAGATGAAGGAGA+TGG | + | chr7.1:28891444-28891463 | MS.gene87690:CDS | 50.0% | |
| ! | GGAGTGTTTATGGGCTGAAG+TGG | - | chr7.1:28891205-28891224 | None:intergenic | 50.0% |
| ! | TTATGGGCTGAAGTGGTGTC+CGG | - | chr7.1:28891198-28891217 | None:intergenic | 50.0% |
| !! | AGATCGGCGGGAGTGTTTAT+GGG | - | chr7.1:28891214-28891233 | None:intergenic | 50.0% |
| !!! | ATTTTTGGTGTTCCGGTGGC+GGG | - | chr7.1:28891160-28891179 | None:intergenic | 50.0% |
| !!! | GATTTTTGGTGTTCCGGTGG+CGG | - | chr7.1:28891161-28891180 | None:intergenic | 50.0% |
| !!! | TGGCCGGATTTTTGGTGTTC+CGG | - | chr7.1:28891167-28891186 | None:intergenic | 50.0% |
| AGATGGTGAGTGAGATCGGC+GGG | - | chr7.1:28891226-28891245 | None:intergenic | 55.0% | |
| CTTATGCAAACACCCGCCAC+CGG | + | chr7.1:28891145-28891164 | MS.gene87690:CDS | 55.0% | |
| TCCGATCACCACTTCTCCAC+TGG | + | chr7.1:28891315-28891334 | MS.gene87690:CDS | 55.0% | |
| !! | GAGATCGGCGGGAGTGTTTA+TGG | - | chr7.1:28891215-28891234 | None:intergenic | 55.0% |
| !!! | CCGGATTTTTGGTGTTCCGG+TGG | - | chr7.1:28891164-28891183 | None:intergenic | 55.0% |
| ACTGGAGACGTCGCTTGCAG+AGG | + | chr7.1:28891333-28891352 | MS.gene87690:CDS | 60.0% | |
| CCAAAAATCCGGCCACGCTC+TGG | + | chr7.1:28891172-28891191 | MS.gene87690:CDS | 60.0% | |
| CGACGTCTCCAGTGGAGAAG+TGG | - | chr7.1:28891326-28891345 | None:intergenic | 60.0% | |
| TCTGCAAGCGACGTCTCCAG+TGG | - | chr7.1:28891334-28891353 | None:intergenic | 60.0% | |
| ! | CCAGAGCGTGGCCGGATTTT+TGG | - | chr7.1:28891175-28891194 | None:intergenic | 60.0% |
| AAATCCGGCCACGCTCTGGC+CGG | + | chr7.1:28891176-28891195 | MS.gene87690:CDS | 65.0% | |
| AGTGGTGTCCGGCCAGAGCG+TGG | - | chr7.1:28891187-28891206 | None:intergenic | 70.0% | |
| GTGTCCGGCCAGAGCGTGGC+CGG | - | chr7.1:28891183-28891202 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 28891136 | 28891722 | 28891136 | ID=MS.gene87690 |
| chr7.1 | mRNA | 28891136 | 28891722 | 28891136 | ID=MS.gene87690.t1;Parent=MS.gene87690 |
| chr7.1 | exon | 28891136 | 28891549 | 28891136 | ID=MS.gene87690.t1.exon1;Parent=MS.gene87690.t1 |
| chr7.1 | CDS | 28891136 | 28891549 | 28891136 | ID=cds.MS.gene87690.t1;Parent=MS.gene87690.t1 |
| chr7.1 | exon | 28891639 | 28891722 | 28891639 | ID=MS.gene87690.t1.exon2;Parent=MS.gene87690.t1 |
| chr7.1 | CDS | 28891639 | 28891722 | 28891639 | ID=cds.MS.gene87690.t1;Parent=MS.gene87690.t1 |
| Gene Sequence |
| Protein sequence |
