Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88200.t1 | XP_024636833.1 | 96.2 | 266 | 10 | 0 | 12 | 277 | 52 | 317 | 3.20E-138 | 501.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88200.t1 | A0A396I6X6 | 96.2 | 266 | 10 | 0 | 12 | 277 | 52 | 317 | 2.3e-138 | 501.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053107 | MS.gene88200 | 0.803211 | 3.88E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88200.t1 | MTR_4g052500 | 96.016 | 251 | 10 | 0 | 11 | 261 | 51 | 301 | 3.73e-166 | 471 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88200.t1 | AT5G22340 | 61.255 | 271 | 96 | 3 | 14 | 277 | 55 | 323 | 1.41e-113 | 330 |
| MS.gene88200.t1 | AT5G22340 | 59.664 | 238 | 87 | 3 | 14 | 244 | 55 | 290 | 1.66e-92 | 277 |
Find 49 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGCAGCAATTTATGGTCTT+TGG | 0.181311 | 4.4:-50084536 | MS.gene88200:CDS |
| GCAGTCATCTTCCTATTTAC+TGG | 0.187800 | 4.4:+50087216 | None:intergenic |
| CTCAGCCTTCAAAATTCTTC+TGG | 0.224073 | 4.4:+50084026 | None:intergenic |
| TAGCTTGTGCCAGCCATGTT+TGG | 0.268429 | 4.4:-50084119 | MS.gene88200:CDS |
| GTGATCCCGGCAGCAATTTA+TGG | 0.287109 | 4.4:-50084543 | MS.gene88200:CDS |
| CTACTTCTCTCTGTGGGTAT+TGG | 0.302680 | 4.4:-50086775 | MS.gene88200:CDS |
| AGTACCCAAGAGTGGTTATT+AGG | 0.309012 | 4.4:-50087252 | MS.gene88200:CDS |
| CTCTCTGTGGGTATTGGATT+GGG | 0.310124 | 4.4:-50086769 | MS.gene88200:CDS |
| TCTCTCTGTGGGTATTGGAT+TGG | 0.323276 | 4.4:-50086770 | MS.gene88200:CDS |
| ACAATTCTGCACCAGTAAAT+AGG | 0.325690 | 4.4:-50087227 | MS.gene88200:CDS |
| GGCAGCAATTTATGGTCTTT+GGG | 0.326533 | 4.4:-50084535 | MS.gene88200:CDS |
| CGAATGGATTACAGAAATTC+TGG | 0.335666 | 4.4:-50087525 | MS.gene88200:CDS |
| CAAGAACTTGTGATTTACTA+TGG | 0.342168 | 4.4:-50087548 | MS.gene88200:CDS |
| AGCTTGTGCCAGCCATGTTT+GGG | 0.354796 | 4.4:-50084118 | MS.gene88200:CDS |
| CATCCAATGTATATTATTAG+AGG | 0.371654 | 4.4:-50095050 | MS.gene88200:intron |
| TGAGTCTTGCTCTGTTGTCT+TGG | 0.381989 | 4.4:+50086983 | None:intergenic |
| CTGCTATAAGTTATGCGATT+GGG | 0.398513 | 4.4:-50086413 | MS.gene88200:CDS |
| ATCTCCAAGGCTTGTGATCC+CGG | 0.413235 | 4.4:-50084556 | MS.gene88200:CDS |
| TGTCGCCTAATAACCACTCT+TGG | 0.416392 | 4.4:+50087247 | None:intergenic |
| ATTCTTTCGAGAAAACAATC+AGG | 0.440956 | 4.4:-50084599 | MS.gene88200:CDS |
| TATAGAGACAATAAAGACTT+AGG | 0.447368 | 4.4:-50084059 | MS.gene88200:CDS |
| ATGCAATAACAAACCTGAAA+AGG | 0.451361 | 4.4:+50086388 | None:intergenic |
| TTGAAGGATAGAGAATTAAA+CGG | 0.472253 | 4.4:-50087582 | MS.gene88200:CDS |
| TATTTGTGGAACAGTTTCAC+TGG | 0.472479 | 4.4:+50084778 | None:intergenic |
| TTGTGATTTACTATGGCGAA+TGG | 0.515409 | 4.4:-50087541 | MS.gene88200:CDS |
| GTCGCCTAATAACCACTCTT+GGG | 0.520653 | 4.4:+50087248 | None:intergenic |
| CAAAGACCATAAATTGCTGC+CGG | 0.523449 | 4.4:+50084537 | None:intergenic |
| CAATGCTCAACAAATAGAAC+AGG | 0.524035 | 4.4:-50087469 | MS.gene88200:intron |
| GAGAAAACAATCAGGGGTAG+TGG | 0.540135 | 4.4:-50084591 | MS.gene88200:CDS |
| TTTAGTGAGTAATGTAGATG+AGG | 0.543159 | 4.4:-50087622 | MS.gene88200:intron |
| GAATTACTACTTCTCTCTGT+GGG | 0.553752 | 4.4:-50086781 | MS.gene88200:CDS |
| GCTGCCGGGATCACAAGCCT+TGG | 0.555710 | 4.4:+50084552 | None:intergenic |
| ACAAACATCCCAAACATGGC+TGG | 0.556898 | 4.4:+50084110 | None:intergenic |
| TGCTATAAGTTATGCGATTG+GGG | 0.558016 | 4.4:-50086412 | MS.gene88200:CDS |
| GTATAGCACTTTCATCTCCA+AGG | 0.559840 | 4.4:-50084569 | MS.gene88200:CDS |
| TTCTTTCGAGAAAACAATCA+GGG | 0.581970 | 4.4:-50084598 | MS.gene88200:CDS |
| GCTGCTATAAGTTATGCGAT+TGG | 0.586026 | 4.4:-50086414 | MS.gene88200:CDS |
| TTACCTCTAATAATATACAT+TGG | 0.588813 | 4.4:+50095047 | None:intergenic |
| CATGTTGATACAAGAGCTGA+AGG | 0.591014 | 4.4:+50084813 | None:intergenic |
| AAAGACCATAAATTGCTGCC+GGG | 0.592930 | 4.4:+50084538 | None:intergenic |
| TTGTTGAGCATTGTTGTCGT+CGG | 0.594031 | 4.4:+50087479 | None:intergenic |
| GGTATTGGATTGGGTTGTAG+CGG | 0.607478 | 4.4:-50086760 | MS.gene88200:CDS |
| AACTTTCAAGTACCCAAGAG+TGG | 0.615621 | 4.4:-50087260 | MS.gene88200:CDS |
| ATCATAGAGACTAACAGCGA+TGG | 0.632346 | 4.4:-50087294 | MS.gene88200:CDS |
| AAATAGGAAGATGACTGCAA+AGG | 0.634207 | 4.4:-50087211 | MS.gene88200:intron |
| GTAGACAAACATCCCAAACA+TGG | 0.634660 | 4.4:+50084106 | None:intergenic |
| AGAATTACTACTTCTCTCTG+TGG | 0.661836 | 4.4:-50086782 | MS.gene88200:CDS |
| ATAGAGACTAACAGCGATGG+CGG | 0.666763 | 4.4:-50087291 | MS.gene88200:CDS |
| TCTTTCGAGAAAACAATCAG+GGG | 0.694510 | 4.4:-50084597 | MS.gene88200:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 50084034 | 50095081 | 50084034 | ID=MS.gene88200 |
| chr4.4 | mRNA | 50084034 | 50095081 | 50084034 | ID=MS.gene88200.t1;Parent=MS.gene88200 |
| chr4.4 | exon | 50095051 | 50095081 | 50095051 | ID=MS.gene88200.t1.exon1;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50095051 | 50095081 | 50095051 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| chr4.4 | exon | 50087470 | 50087639 | 50087470 | ID=MS.gene88200.t1.exon2;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50087470 | 50087639 | 50087470 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| chr4.4 | exon | 50087212 | 50087316 | 50087212 | ID=MS.gene88200.t1.exon3;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50087212 | 50087316 | 50087212 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| chr4.4 | exon | 50086954 | 50087010 | 50086954 | ID=MS.gene88200.t1.exon4;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50086954 | 50087010 | 50086954 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| chr4.4 | exon | 50086732 | 50086812 | 50086732 | ID=MS.gene88200.t1.exon5;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50086732 | 50086812 | 50086732 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| chr4.4 | exon | 50086402 | 50086436 | 50086402 | ID=MS.gene88200.t1.exon6;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50086402 | 50086436 | 50086402 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| chr4.4 | exon | 50084755 | 50084844 | 50084755 | ID=MS.gene88200.t1.exon7;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50084755 | 50084844 | 50084755 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| chr4.4 | exon | 50084491 | 50084650 | 50084491 | ID=MS.gene88200.t1.exon8;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50084491 | 50084650 | 50084491 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| chr4.4 | exon | 50084034 | 50084138 | 50084034 | ID=MS.gene88200.t1.exon9;Parent=MS.gene88200.t1 |
| chr4.4 | CDS | 50084034 | 50084138 | 50084034 | ID=cds.MS.gene88200.t1;Parent=MS.gene88200.t1 |
| Gene Sequence |
| Protein sequence |
