Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053107.t1 | XP_013450693.1 | 88.1 | 303 | 25 | 2 | 1 | 294 | 1 | 301 | 1.30E-140 | 509.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| No data available in table | |||||||||||
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053107.t1 | A0A072UG11 | 88.1 | 303 | 25 | 2 | 1 | 294 | 1 | 301 | 9.1e-141 | 509.2 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene053107 | 0.819142 | 1.36E-52 | -1.69E-46 |
| MS.gene049217 | MS.gene053107 | 0.815806 | 7.65E-52 | -1.69E-46 |
| MS.gene049222 | MS.gene053107 | 0.828805 | 7.33E-55 | -1.69E-46 |
| MS.gene049285 | MS.gene053107 | 0.806364 | 8.53E-50 | -1.69E-46 |
| MS.gene049351 | MS.gene053107 | 0.817408 | 3.35E-52 | -1.69E-46 |
| MS.gene049427 | MS.gene053107 | 0.883427 | 4.53E-71 | -1.69E-46 |
| MS.gene049609 | MS.gene053107 | 0.810329 | 1.22E-50 | -1.69E-46 |
| MS.gene049615 | MS.gene053107 | 0.827534 | 1.48E-54 | -1.69E-46 |
| MS.gene050163 | MS.gene053107 | 0.804477 | 2.12E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene053107 | 0.833675 | 4.65E-56 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053107.t1 | MTR_6g004030 | 91.776 | 304 | 12 | 4 | 1 | 294 | 1 | 301 | 0.0 | 544 |
| MS.gene053107.t1 | MTR_6g004030 | 89.474 | 247 | 13 | 4 | 1 | 237 | 1 | 244 | 2.30e-152 | 426 |
| MS.gene053107.t1 | MTR_8g046180 | 43.750 | 192 | 108 | 0 | 103 | 294 | 127 | 318 | 3.58e-57 | 186 |
| MS.gene053107.t1 | MTR_8g046180 | 44.444 | 162 | 90 | 0 | 101 | 262 | 125 | 286 | 1.59e-46 | 158 |
| MS.gene053107.t1 | MTR_8g046180 | 42.708 | 96 | 55 | 0 | 101 | 196 | 125 | 220 | 5.55e-22 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053107.t1 | AT5G08400 | 74.615 | 260 | 65 | 1 | 36 | 294 | 41 | 300 | 1.18e-152 | 429 |
| MS.gene053107.t1 | AT5G08400 | 66.212 | 293 | 65 | 2 | 36 | 294 | 41 | 333 | 1.18e-145 | 412 |
| MS.gene053107.t1 | AT4G29400 | 42.784 | 194 | 111 | 0 | 101 | 294 | 109 | 302 | 2.22e-59 | 192 |
Find 68 sgRNAs with CRISPR-Local
Find 238 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACACAGAACGAGTAGTAGA+AGG | 0.460648 | 6.2:-59703 | None:intergenic |
| AACCAGATATATGAATCAAG+TGG | 0.567513 | 6.2:-60631 | None:intergenic |
| AAGTACTTGTAACCATACTC+AGG | 0.526116 | 6.2:-63545 | None:intergenic |
| AATACTTACTTAGCCCAAGC+AGG | 0.499987 | 6.2:-59883 | None:intergenic |
| ACCGAGGATGATGACTGTGG+GGG | 0.704905 | 6.2:-59667 | None:intergenic |
| ACTCAACATCACTGATATCA+TGG | 0.462409 | 6.2:-62061 | None:intergenic |
| ACTCGTTCCTTCATGAGTTG+AGG | 0.531698 | 6.2:-60083 | None:intergenic |
| ACTCTGAGCTTAAATTAGTA+AGG | 0.421880 | 6.2:-63325 | None:intergenic |
| ACTGAACTACGTGCTGTACT+TGG | 0.518691 | 6.2:+60044 | MS.gene053107:CDS |
| AGAAAGTGAGATGAGTAGCA+TGG | 0.631747 | 6.2:+59612 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGGAGAAACTAAATGATC+AGG | - | chr6.2:62521-62540 | None:intergenic | 30.0% | |
| AACCAGATATATGAATCAAG+TGG | - | chr6.2:60634-60653 | None:intergenic | 30.0% | |
| AAGACCACATACATAGTAAA+TGG | + | chr6.2:60448-60467 | MS.gene053107:intron | 30.0% | |
| AAGCATGTAATGAATGAATG+GGG | - | chr6.2:60861-60880 | None:intergenic | 30.0% | |
| ACTCTGAGCTTAAATTAGTA+AGG | - | chr6.2:63328-63347 | None:intergenic | 30.0% | |
| ATAGCTACACTTCAAAAAGA+GGG | - | chr6.2:61437-61456 | None:intergenic | 30.0% | |
| ATATAATCAACTTCACCTAG+TGG | + | chr6.2:62980-62999 | MS.gene053107:intron | 30.0% | |
| ATCATCATTATCCTTCAAGT+TGG | - | chr6.2:59755-59774 | None:intergenic | 30.0% | |
| ATCCACTTGATTCATATATC+TGG | + | chr6.2:60629-60648 | MS.gene053107:CDS | 30.0% | |
| ATGAGACCACAAAAAACTAT+AGG | - | chr6.2:62335-62354 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 59622 | 63577 | 59622 | ID=MS.gene053107 |
| chr6.2 | mRNA | 59622 | 63577 | 59622 | ID=MS.gene053107.t1;Parent=MS.gene053107 |
| chr6.2 | exon | 59622 | 59896 | 59622 | ID=MS.gene053107.t1.exon1;Parent=MS.gene053107.t1 |
| chr6.2 | CDS | 59622 | 59896 | 59622 | ID=cds.MS.gene053107.t1;Parent=MS.gene053107.t1 |
| chr6.2 | exon | 60016 | 60103 | 60016 | ID=MS.gene053107.t1.exon2;Parent=MS.gene053107.t1 |
| chr6.2 | CDS | 60016 | 60103 | 60016 | ID=cds.MS.gene053107.t1;Parent=MS.gene053107.t1 |
| chr6.2 | exon | 60195 | 60280 | 60195 | ID=MS.gene053107.t1.exon3;Parent=MS.gene053107.t1 |
| chr6.2 | CDS | 60195 | 60280 | 60195 | ID=cds.MS.gene053107.t1;Parent=MS.gene053107.t1 |
| chr6.2 | exon | 60624 | 60702 | 60624 | ID=MS.gene053107.t1.exon4;Parent=MS.gene053107.t1 |
| chr6.2 | CDS | 60624 | 60702 | 60624 | ID=cds.MS.gene053107.t1;Parent=MS.gene053107.t1 |
| chr6.2 | exon | 60791 | 60850 | 60791 | ID=MS.gene053107.t1.exon5;Parent=MS.gene053107.t1 |
| chr6.2 | CDS | 60791 | 60850 | 60791 | ID=cds.MS.gene053107.t1;Parent=MS.gene053107.t1 |
| chr6.2 | exon | 61987 | 62108 | 61987 | ID=MS.gene053107.t1.exon6;Parent=MS.gene053107.t1 |
| chr6.2 | CDS | 61987 | 62108 | 61987 | ID=cds.MS.gene053107.t1;Parent=MS.gene053107.t1 |
| chr6.2 | exon | 63274 | 63345 | 63274 | ID=MS.gene053107.t1.exon7;Parent=MS.gene053107.t1 |
| chr6.2 | CDS | 63274 | 63345 | 63274 | ID=cds.MS.gene053107.t1;Parent=MS.gene053107.t1 |
| chr6.2 | exon | 63475 | 63577 | 63475 | ID=MS.gene053107.t1.exon8;Parent=MS.gene053107.t1 |
| chr6.2 | CDS | 63475 | 63577 | 63475 | ID=cds.MS.gene053107.t1;Parent=MS.gene053107.t1 |
| Gene Sequence |
>MS.gene053107 ATGAGTAGCATGGCGTGTGGATGTTGGTACTACAACTACTGCTGTCCCCCACAGTCATCATCCTCGGTGCTGAAGCGTAGTCCTTCTACTACTCGTTCTGTGTTTGTTTGTTGTGTCTCTAAATCCAAATCCAACTTGAAGGATAATGATGATGAAATTGATAAATCGATAAAAGGATCAGGAACCACTGCTAGAGGTCGAAGATTACTAAGAATTCGACAAGATAAACAACAACGCGAACACGAACGCCTTCACAATTACCCTGCTTGGGCTAAGTAAGTATTAGTAGTACTTTCTTTCTTTCTTTCTTTCTTTTTTATTATTGCATTATTGTATTTTATTTGAAGTATATATATGGTTTCTGAATGAATGAATATTTGCATAATAATGATAGAGTCCTTGAAGATGCTTGCAAAAACGACACTGAACTACGTGCTGTACTTGGTGATAGTATTGGCAACCCTCAACTCATGAAGGAACGAGTATGTTATCTTATGTTATCTTCTTTCTTACTTGCTACTTCCTTATTTTCAATTCTAATTAAATTTAATGTGTTCCTTTCTTTCCTTCCAGGTGGAAGATAGAATTCGAAAAAAGGGTAAAGACTTTCGTCGCTCCAAAACTGGATCTGTTGTTGCCTCCAAAGTCACCTTTAGAGAGTATGTATTATCATAATAATTAATGTTTTTGCTTCGTCTATGACTTTGTAGGGAGCTCTCAAATAGATTCATATAGTTATGAATATTTCATAAAACTCCCGGCTCTGTCAAATTTTTACTCATATTAGTCCTTCTAAGAGTCCAAAACTAAATTTGATAGTAGGTTAAAGACCACATACATAGTAAATGGTTAAAATATAGAGATCTAATTAATAGTTTAATAGAGTCGATGATCTGTTTGAAAAATTCATACTCTCAGAGACCTACTTGGTGGTATACTCTTCTAAATTTGTTCTCGCATTACTCTAATTATTATTTGTCATGGGATGCCTTTTGCTCGCAGCTTCAATCCACTTGATTCATATATCTGGTTTGAATTGTATGGATCACCATCTGATCGCGACGTTAATCTAATTGGCAGTGTATGTCTTTTCTTCTTTAGCTTTTCAATCTATATAATACATTCATCAAATCTTATTCTTTCATTCATGTCAATTAATTATTATATAGGTTATTCAAGCATGGTATGTCATGGGTCGACTTGGGGCCTACAATTCTTCAAACTTGCAGGTACTTACCCCATTCATTCATTACATGCTTACTATCGTCAATTCCTCCCACTCACTTTCATTTGCTACTTCTTCAGTTCTTTTAGGTATGCCTTACCTTAATGAAATCATCAAATCTACTTTATTTTGGTTCCGTTGACTTGAAATTCCTCAAAATTCTGGCACCAATTTTGGTATTTAATTCAAATTTATAATGGAGAGAGCCAAGCAAGCATTGTAGTAGAGAGTGAAATTGAGACGCCCGAACCCAATTGGCTTATGAGTTTTATACACATGTACTTTTATAAAAGTACTAAATAGCAAATACCTCCTGGAATATTATAATTCTTACCAGTTTAGTAGTCTTTATGTTAGTAGATAGTAGTTTCAATTGGTTGTCCAAGTTGTACTAGGTACGGTTCATACTTGACCAAAATAATTAGTTCAAGTAAGAGTATTACAGTCGCGATCATAGCAGAGTATTCTTGTTTATTGATAACTAGGTAAGAGCTTAAGTAGCTTTCATGCTTCATATAGACAGGGAATATTACAATTAAGATTATTATTAGTTGTTATACCTTATTGGACTGAGCTTTTCATGCTTTCCCTCTTTTTGAAGTGTAGCTATATGATTGAAATTACATGTCTAGATTTCCTATTTGCATGTTTTTCTTAAAGAGAACTTTTACATTATGGAATAGCATGAGCTCAGTACTATAACTGTGAAAATTTCCTTACAAATTTTAGGAGTGAAGTTTATGTGTTGAGGTGACTTTTTGTGTGATAGTATAGTACATGCACGTTATTGTATTCGTTATGTAATTACATTTAACCATCTGACAGCACATGCTTACACTTACACGTTAGCGACAACTAGCTCGAAAATATTTTGGCGATTAGATTTGTATTGCTAATAATATCCATGTCTCTTCTGATACAAGTTCTAGGATCATTTTCTCTGCCTATCAGAATGTTTACAAAATAGCAAGAACAGCAACAATGGAAGACACTGTAAAAGTTTAATTAATTTTACTTTTTGGATTGTCTGATTGCTTCGGCTATATGTGTCATAGAATTATCATTCATTTTTAGTTTCCAACCTGTGTGTGGCTGCTCCCTTTTAACAAAGAATTGAAGTATCTTTTTAATTGATGGCAGATGTCAAATTCGTCAATGGAGTATGATCCTCTCTATGACTCAGATAAGGGTTTCAAAGTGATGCCGTCATCTTTCCATGATATCAGTGATGTTGAGTTCCAGGATAATTGGGGGCGTGTTTGGTATATTGTCTAAAACTCTGTTAGTCTTTTTAATAAGTGGTTTTATGACCTTGCAATGGACAGAGGTTGAAAAAATAGAATGATGCAGATTAAATTATTAACAACATTAACCACAATAAAATGCCTTTTCACTCTAGGAGGAACTGACAACATGGATTCTATGATATCATAAATCATGTCTAGTGACAAACTATTTAAACCTAAAACCTTTTTACTAGTTTAGCCTATAGTTTTTTGTGGTCTCATTTTTCCTCTAACTAATGGACTTTTCTCCATTTGATTTACTCTTCTTAGCGAGGCTTTTAGAGATCTTCTCCTCAGTACACACATGTCTAAATTACCTAGGACTAGTTTTGACCATCTTTTCTGAATAGGAGGCTACCCCAACTTTCTCTTCAAAGTAACCACTCCTGATCATTTAGTTTCTCCTTTATAGCTTTTATTGACTTTTCAATTATGAGTATACCATTCGGAAAAAGGCATTATCGTGGCATTGTCTCTTGGATATGTTTCGCAGGAACATGAAATAAAACCAAGCTCAGTGTTGCAGAGATGAGAGTATTACGATAGGCGAGTTATTACTTACAAAAAAAAAGAAACAAGATTGTATCAAGAGAGAAACCACAAGAGAAAAAATAGGGTAGCTCCCAAAAGAGAAAGGATGCTAAAGTTGTGTCTTGTATTGTTTAGAGAGCGGGGAAAACTAGTTGAAACACCGGTATGAAGGATAGACCAGATGGAGAATAGTTCAATTGTTAGAGGTACAGGGAGACCCAGAAAAATTATTGGTCATATCATTAAAAAAGATGTATATTTAAATGGGATATCATTAGACTTGATTGGCAACAAAACATTGTGGATTATTTGATTCATATAATCAACTTCACCTAGTGGAAAAAACAAAGGCTTCGATGGTTGTCACCAGCTAAATAGGTATTGAAAAGGAGGGAATTTTTCTTTATTATACAATTTCTAGTAACTGAAAGTTTCAGAATTATAATTAAAGTTGTAGAGACTTAATAATTTAATACACAAACAATTAATAACAATGTCATTTAGTTAAATACATGACTGCAGTAGAATATGAAAACACGGTAGGATATATTTTCAATCAAAACTCATGTTGGTTCAATATGTTTAAGCTTTAAACAAATCGTACTTGCAGGGTGGACCTCGGTACTGCTGATTATTTTGCGATTGATGTGCTTCTCAACTGCCTTACTAATTTAAGCTCAGAGTAAGTTTGAATTACTGAACTACATGTAATAAAAATGTAAAGAATATATGTAATGTAACTATATGTCCAAGCGCGCATTTATTGTTTTTGTTATGCAAAAGTAATGATTGACCTTTCTCTGAATGGCAGATATCTTGGTATCCAACAAATTGTTTTTGGTGGTCGGTCCATGGGTGATTGGGAAGAGGGGATGACAAACCCTGAGTATGGTTACAAGTACTTCAAGATATGA |
| Protein sequence |
>MS.gene053107.t1 MSSMACGCWYYNYCCPPQSSSSVLKRSPSTTRSVFVCCVSKSKSNLKDNDDEIDKSIKGSGTTARGRRLLRIRQDKQQREHERLHNYPAWAKVLEDACKNDTELRAVLGDSIGNPQLMKERVEDRIRKKGKDFRRSKTGSVVASKVTFRDFNPLDSYIWFELYGSPSDRDVNLIGSVIQAWYVMGRLGAYNSSNLQMSNSSMEYDPLYDSDKGFKVMPSSFHDISDVEFQDNWGRVWVDLGTADYFAIDVLLNCLTNLSSEYLGIQQIVFGGRSMGDWEEGMTNPEYGYKYFKI |
