AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88261

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88261.t1 MTR_4g033085 97.134 314 9 0 1 314 1 314 0.0 626
MS.gene88261.t1 MTR_4g033170 96.497 314 11 0 1 314 1 314 0.0 623
MS.gene88261.t1 MTR_4g033200 76.730 318 68 1 1 312 1 318 0.0 511
MS.gene88261.t1 MTR_4g088200 77.287 317 68 1 1 313 1 317 0.0 509
MS.gene88261.t1 MTR_4g033015 76.415 318 69 1 1 312 1 318 0.0 508
MS.gene88261.t1 MTR_4g033150 74.214 318 76 1 1 312 1 318 5.21e-178 497
MS.gene88261.t1 MTR_4g088190 74.766 321 74 1 1 314 1 321 4.66e-171 479
MS.gene88261.t1 MTR_0794s0020 73.494 249 60 1 1 243 1 249 4.63e-137 389
MS.gene88261.t1 MTR_7g014570 49.045 314 156 3 6 319 6 315 1.37e-106 315
MS.gene88261.t1 MTR_7g014510 46.541 318 163 3 6 319 6 320 1.37e-101 303
MS.gene88261.t1 MTR_1g076940 47.678 323 158 5 5 319 5 324 5.26e-99 296
MS.gene88261.t1 MTR_7g024500 45.687 313 162 4 6 317 5 310 5.10e-97 291
MS.gene88261.t1 MTR_4g088345 48.418 316 158 4 1 314 1 313 3.40e-95 286
MS.gene88261.t1 MTR_7g024520 45.367 313 163 4 6 317 5 310 1.37e-94 285
MS.gene88261.t1 MTR_5g074680 44.201 319 167 6 3 313 1 316 1.43e-93 283
MS.gene88261.t1 MTR_5g074600 44.728 313 165 5 11 318 11 320 4.94e-93 281
MS.gene88261.t1 MTR_5g074580 43.175 315 171 5 4 313 5 316 3.02e-88 269
MS.gene88261.t1 MTR_5g074540 41.311 305 159 5 11 313 11 297 6.52e-85 259
MS.gene88261.t1 MTR_5g074610 42.810 306 142 3 11 313 7 282 1.73e-84 258
MS.gene88261.t1 MTR_4g088340 47.099 293 150 4 24 314 1 290 4.45e-84 257
MS.gene88261.t1 MTR_4g079160 41.776 304 174 2 11 313 10 311 3.16e-83 256
MS.gene88261.t1 MTR_1g076930 45.833 240 121 5 24 257 1 237 2.34e-59 191
MS.gene88261.t1 MTR_3g083620 35.314 303 182 9 20 317 27 320 1.49e-47 164
MS.gene88261.t1 MTR_0017s0100 33.121 314 203 6 3 313 14 323 4.98e-46 160
MS.gene88261.t1 MTR_4g038440 32.803 314 204 6 3 313 14 323 3.15e-45 158
MS.gene88261.t1 MTR_0079s0070 32.990 291 188 6 26 313 3 289 7.13e-41 145
MS.gene88261.t1 MTR_5g098170 34.586 266 156 9 31 284 33 292 4.18e-35 131
MS.gene88261.t1 MTR_0591s0010 30.249 281 178 7 26 295 41 314 4.34e-33 126
MS.gene88261.t1 MTR_1g096310 30.000 320 202 8 5 312 17 326 3.81e-32 123
MS.gene88261.t1 MTR_7g118320 31.481 270 170 6 26 284 26 291 4.37e-32 123
MS.gene88261.t1 MTR_8g068870 28.428 299 196 7 5 292 6 297 8.06e-32 122
MS.gene88261.t1 MTR_3g092900 31.153 321 184 10 9 312 17 317 9.93e-32 122
MS.gene88261.t1 MTR_7g118300 30.545 275 176 8 26 292 42 309 2.37e-31 121
MS.gene88261.t1 MTR_2g072790 33.333 291 177 8 31 313 27 308 6.13e-31 120
MS.gene88261.t1 MTR_8g068690 29.893 281 179 7 26 295 40 313 6.36e-31 120
MS.gene88261.t1 MTR_7g012090 29.242 277 173 7 32 292 40 309 1.49e-30 119
MS.gene88261.t1 MTR_7g011900 29.371 286 186 7 32 307 40 319 2.11e-30 119
MS.gene88261.t1 MTR_8g068750 29.268 287 185 7 26 301 41 320 3.10e-30 118
MS.gene88261.t1 MTR_7g012040 28.889 270 177 7 32 292 39 302 9.22e-30 117
MS.gene88261.t1 MTR_1g036460 29.831 295 189 7 24 306 29 317 1.39e-29 116
MS.gene88261.t1 MTR_7g012070 29.348 276 174 7 32 292 40 309 1.45e-29 116
MS.gene88261.t1 MTR_1g036490 29.492 295 190 7 24 306 29 317 2.35e-29 115
MS.gene88261.t1 MTR_4g038440 30.620 258 175 4 3 257 14 270 4.87e-29 112
MS.gene88261.t1 MTR_7g011990 26.978 278 186 7 32 300 40 309 2.83e-28 112
MS.gene88261.t1 MTR_7g012020 29.924 264 170 7 32 284 39 298 3.29e-28 112
MS.gene88261.t1 MTR_8g068570 30.357 280 152 8 26 295 41 287 3.83e-28 111
MS.gene88261.t1 MTR_2g055940 30.216 278 167 9 32 294 34 299 1.37e-27 110
MS.gene88261.t1 MTR_3g021440 30.928 291 182 9 28 305 38 322 1.43e-26 108
MS.gene88261.t1 MTR_8g024160 32.195 205 131 4 89 292 65 262 7.07e-26 105
MS.gene88261.t1 MTR_1g036510 28.956 297 187 10 25 306 44 331 1.77e-24 102
MS.gene88261.t1 MTR_6g078850 26.298 289 191 7 9 284 33 312 1.78e-23 100
MS.gene88261.t1 MTR_3g021430 29.010 293 187 10 32 312 29 312 1.62e-22 97.1
MS.gene88261.t1 MTR_7g012020 28.631 241 157 7 32 261 39 275 3.24e-21 92.4
MS.gene88261.t1 MTR_3g096050 27.181 298 201 10 23 313 24 312 5.54e-21 92.4
MS.gene88261.t1 MTR_8g068730 30.000 270 142 8 26 284 41 274 7.76e-21 92.0
MS.gene88261.t1 MTR_3g096070 25.676 296 185 9 23 313 24 289 8.04e-19 86.3
MS.gene88261.t1 MTR_8g068690 26.423 246 166 6 26 260 40 281 8.83e-19 85.1
MS.gene88261.t1 MTR_8g068740 27.839 273 150 7 26 287 41 277 2.27e-17 81.3
MS.gene88261.t1 MTR_8g068600 25.862 232 157 6 38 258 27 254 3.17e-17 80.9
MS.gene88261.t1 MTR_1g036510 28.629 248 156 9 25 257 44 285 3.66e-15 75.1
MS.gene88261.t1 MTR_8g068620 33.929 112 72 1 149 260 4 113 3.75e-14 68.6
MS.gene88261.t1 MTR_4g025570 38.776 98 52 3 6 101 16 107 7.76e-14 70.1
MS.gene88261.t1 MTR_7g012020 28.571 210 135 7 32 230 39 244 2.40e-13 69.3
MS.gene88261.t1 MTR_1g050220 33.613 119 66 2 188 306 147 252 2.33e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88261.t1 AT4G35150 30.323 310 159 8 16 313 15 279 2.47e-36 134
MS.gene88261.t1 AT5G54160 32.526 289 181 6 31 312 34 315 2.88e-36 134
MS.gene88261.t1 AT4G35160 32.188 320 191 10 16 317 29 340 3.16e-34 129
MS.gene88261.t1 AT1G77520 33.453 278 162 7 31 292 44 314 5.45e-33 126
MS.gene88261.t1 AT3G53140 31.987 297 178 10 31 313 27 313 6.64e-31 120
MS.gene88261.t1 AT5G53810 31.915 282 168 8 29 293 38 312 6.07e-27 109
MS.gene88261.t1 AT1G63140 29.870 308 190 9 9 297 19 319 8.37e-27 109
MS.gene88261.t1 AT1G63140 29.870 308 190 9 9 297 39 339 8.64e-27 109
MS.gene88261.t1 AT1G33030 27.645 293 194 8 31 313 21 305 1.96e-26 107
MS.gene88261.t1 AT1G51990 28.571 287 184 8 33 309 32 307 2.58e-25 104
MS.gene88261.t1 AT1G77530 32.258 279 166 7 31 293 44 315 6.13e-25 103
MS.gene88261.t1 AT1G51990 28.571 287 184 8 33 309 32 307 2.97e-24 102
MS.gene88261.t1 AT1G76790 27.077 325 204 10 7 313 9 318 1.40e-21 94.4
MS.gene88261.t1 AT1G62900 36.641 131 80 1 167 297 16 143 2.98e-19 85.1
MS.gene88261.t1 AT1G21130 25.753 299 194 9 31 313 40 326 1.97e-16 79.7
MS.gene88261.t1 AT1G21110 25.084 299 196 9 31 313 40 326 2.96e-16 79.3
MS.gene88261.t1 AT1G21120 25.418 299 195 9 31 313 40 326 2.96e-16 79.3
MS.gene88261.t1 AT1G21120 25.418 299 195 9 31 313 88 374 4.48e-16 79.0
MS.gene88261.t1 AT1G21100 25.753 299 194 9 31 313 40 326 5.63e-16 78.2
MS.gene88261.t1 AT5G37170 30.000 150 100 3 166 313 43 189 8.02e-16 75.9
MS.gene88261.t1 AT5G37170 31.783 129 84 2 166 293 143 268 1.20e-15 77.0
MS.gene88261.t1 AT5G37170 32.824 131 80 4 188 313 14 141 5.38e-15 72.8
MS.gene88261.t1 AT1G63140 27.612 268 171 8 9 257 39 302 2.36e-14 72.8
MS.gene88261.t1 AT1G33030 26.160 237 160 7 31 257 21 252 9.56e-14 70.5
MS.gene88261.t1 AT1G33030 26.160 237 160 7 31 257 21 252 1.70e-13 70.1

Find 65 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATACATGTTTGTATAAA+TGG 0.173388 4.3:-67049156 None:intergenic
ACTGTTCTTCAATATCTTTA+AGG 0.222796 4.3:-67050756 None:intergenic
TTCAAAGATGAGGCTAATTC+AGG 0.265492 4.3:-67049265 None:intergenic
TATAGGACACTAACTTTAGA+AGG 0.276793 4.3:-67049295 None:intergenic
CAGCCATGTGTTTGAGGGTT+TGG 0.284838 4.3:+67049660 MS.gene88261:CDS
GTTTGAGGGTTTGGAGTCTT+TGG 0.303653 4.3:+67049669 MS.gene88261:CDS
CTCAGAGAACCATTTCTTAG+AGG 0.305502 4.3:-67049491 None:intergenic
GTTGATGTGGCAGGTGGGAC+TGG 0.337992 4.3:+67049691 MS.gene88261:CDS
CTAAAGTTAGTGTCCTATAT+AGG 0.347448 4.3:+67049299 MS.gene88261:CDS
TCATTGGCTTTCCATGTTTG+TGG 0.380132 4.3:-67049237 None:intergenic
ACTAACAAGTGGTGCAAAAC+AGG 0.393012 4.3:-67049431 None:intergenic
AAAGAAGCAATTTCAAGAAA+AGG 0.393418 4.3:+67050779 MS.gene88261:CDS
CCACTGATGAGAAGCTTTGA+AGG 0.393766 4.3:-67049400 None:intergenic
CAGGTTGTGGCTAACTTATC+TGG 0.396383 4.3:+67049778 MS.gene88261:CDS
GAGAGACTTGAAGCCGCATA+TGG 0.397843 4.3:-67050987 None:intergenic
GCACTTTATGAAAGTGCAAC+GGG 0.400827 4.3:+67049526 MS.gene88261:CDS
GATAAACTTAGTTTGTTTCA+AGG 0.401061 4.3:+67049589 MS.gene88261:CDS
CAAAATTTGAACTTTGTTGG+TGG 0.427047 4.3:+67049805 MS.gene88261:CDS
ACTCAAAATTTGAACTTTGT+TGG 0.429435 4.3:+67049802 MS.gene88261:CDS
GCTAATTCAGGAACAGTCAT+TGG 0.438437 4.3:-67049253 None:intergenic
AAGAAGCAATTTCAAGAAAA+GGG 0.443928 4.3:+67050780 MS.gene88261:CDS
AATGTTCAAGCTTGCTCTCA+AGG 0.450410 4.3:+67049633 MS.gene88261:CDS
TGTGGCACTCCTCTAAGAAA+TGG 0.452397 4.3:+67049482 MS.gene88261:CDS
GATTGCAGCCATGTGTTTGA+GGG 0.455193 4.3:+67049655 MS.gene88261:CDS
ATAAACTTAGTTTGTTTCAA+GGG 0.456335 4.3:+67049590 MS.gene88261:CDS
AGTGCTTCATCCATGTTCTC+TGG 0.459421 4.3:+67049456 MS.gene88261:CDS
GCAAGCGCAAGAACCTATAT+AGG 0.464145 4.3:-67049312 None:intergenic
GGATTGCAGCCATGTGTTTG+AGG 0.478258 4.3:+67049654 MS.gene88261:CDS
GAGTCTTTGGTTGATGTGGC+AGG 0.479885 4.3:+67049682 MS.gene88261:CDS
ATCCATGTTCTCTGGATATG+TGG 0.500274 4.3:+67049464 MS.gene88261:CDS
TCTTTGGTTGATGTGGCAGG+TGG 0.504535 4.3:+67049685 MS.gene88261:CDS
CTCAAATCAGCTGTGGAACT+AGG 0.506689 4.3:+67049199 MS.gene88261:CDS
TTTAGATCAACCACAGGTTG+TGG 0.518679 4.3:+67049765 MS.gene88261:CDS
CACAGCTGATTTGAGTGCCA+TGG 0.520024 4.3:-67049191 None:intergenic
GAAATGGTTCTCTGAGGAGA+AGG 0.549990 4.3:+67049498 MS.gene88261:CDS
TGCACTTTATGAAAGTGCAA+CGG 0.553435 4.3:+67049525 MS.gene88261:CDS
TTTGGAGTCTTTGGTTGATG+TGG 0.555368 4.3:+67049678 MS.gene88261:CDS
TTGAGTAAAACAGCATCTGC+AGG 0.557051 4.3:-67049850 None:intergenic
TAAACTTAGTTTGTTTCAAG+GGG 0.566712 4.3:+67049591 MS.gene88261:CDS
AGAGACTTGAAGCCGCATAT+GGG 0.571433 4.3:-67050986 None:intergenic
ATACAGCTTTATAAGTTCCA+TGG 0.573273 4.3:+67049174 MS.gene88261:CDS
ACAATGGATTTCAAGAGCAA+TGG 0.576228 4.3:+67049100 None:intergenic
CTTTGGTTGATGTGGCAGGT+GGG 0.578821 4.3:+67049686 MS.gene88261:CDS
GTTGCAATTGCACTTTGACA+TGG 0.578956 4.3:+67050871 MS.gene88261:CDS
CCTTCAAAGCTTCTCATCAG+TGG 0.586184 4.3:+67049400 MS.gene88261:CDS
TGCCACATATCCAGAGAACA+TGG 0.593078 4.3:-67049466 None:intergenic
CATTTGTGAATCAGCTGCCA+TGG 0.594995 4.3:-67049614 None:intergenic
GTCAAAGTGCAATTGCAACT+CGG 0.597693 4.3:-67050867 None:intergenic
TAGTTTGTTTCAAGGGGCCA+TGG 0.599443 4.3:+67049597 MS.gene88261:CDS
AGGGTGAAGTTTCAAAGATG+AGG 0.607728 4.3:-67049275 None:intergenic
GATAAGTTAGCCACAACCTG+TGG 0.609880 4.3:-67049775 None:intergenic
AGTAAAACAGCATCTGCAGG+AGG 0.624714 4.3:-67049847 None:intergenic
TATAAGATTACTCCCATATG+CGG 0.627092 4.3:+67050974 MS.gene88261:CDS
GTAGATGCAATCCACAAACA+TGG 0.627357 4.3:+67049226 MS.gene88261:CDS
ATAGGACACTAACTTTAGAA+GGG 0.629455 4.3:-67049294 None:intergenic
CATGGCACTCAAATCAGCTG+TGG 0.632130 4.3:+67049192 MS.gene88261:CDS
ACTCCAAACCCTCAAACACA+TGG 0.632186 4.3:-67049663 None:intergenic
CACTTTATGAAAGTGCAACG+GGG 0.648505 4.3:+67049527 MS.gene88261:CDS
TTGCGCTTGCTAACACACGA+TGG 0.651331 4.3:+67049325 MS.gene88261:CDS
AAAACTGTGCATTTGATGTG+AGG 0.654544 4.3:-67049745 None:intergenic
TGAAGCACTCCACTAACAAG+TGG 0.666600 4.3:-67049442 None:intergenic
CTCTAAGAAATGGTTCTCTG+AGG 0.677453 4.3:+67049492 MS.gene88261:CDS
ACTTTATGAAAGTGCAACGG+GGG 0.680144 4.3:+67049528 MS.gene88261:CDS
GTAAAACAGCATCTGCAGGA+GGG 0.681270 4.3:-67049846 None:intergenic
CTGATGAGAAGCTTTGAAGG+AGG 0.702373 4.3:-67049397 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATTATTTTAATTTAT+TGG + chr4.3:67050653-67050672 MS.gene88261:intron 0.0%
!! AAAATTCATATTTAATTCAT+TGG - chr4.3:67050345-67050364 None:intergenic 10.0%
!! TTTCATTAAAAAAAATATGA+AGG + chr4.3:67050471-67050490 MS.gene88261:intron 10.0%
!!! AATATGAATTTTTATGTTTT+TGG + chr4.3:67050353-67050372 MS.gene88261:intron 10.0%
!!! ATTTTACAATTTTATTTCTT+TGG - chr4.3:67050635-67050654 None:intergenic 10.0%
!!! TTTTACAATTTTATTTCTTT+GGG - chr4.3:67050634-67050653 None:intergenic 10.0%
!! TTTGTTAAGAAAGTTAAAAA+AGG - chr4.3:67049557-67049576 None:intergenic 15.0%
!! ACAAATTTATATCTTTGATC+AGG - chr4.3:67050298-67050317 None:intergenic 20.0%
!! AGGTGATAAAATAATAACAT+CGG + chr4.3:67050035-67050054 MS.gene88261:intron 20.0%
!! ATCCAAAAAAAAATTACTTG+TGG + chr4.3:67049962-67049981 MS.gene88261:intron 20.0%
!! CAAAATAAAATGGAATCAAA+TGG - chr4.3:67049902-67049921 None:intergenic 20.0%
!! TATGTTCTGATTTCATAATT+AGG + chr4.3:67049928-67049947 MS.gene88261:intron 20.0%
!! TCCAAAAAAAAATTACTTGT+GGG + chr4.3:67049963-67049982 MS.gene88261:intron 20.0%
!! TGATCAAAGATATAAATTTG+TGG + chr4.3:67050297-67050316 MS.gene88261:intron 20.0%
!! TGTATACATGTTTGTATAAA+TGG - chr4.3:67049159-67049178 None:intergenic 20.0%
!!! ACGTAAGAACAATTTTTATT+TGG - chr4.3:67050170-67050189 None:intergenic 20.0%
!!! ATAAACTTAGTTTGTTTCAA+GGG + chr4.3:67049590-67049609 MS.gene88261:CDS 20.0%
!!! TCATAGATGAAAAATTTTGA+GGG + chr4.3:67050531-67050550 MS.gene88261:intron 20.0%
!!! TTCATAGATGAAAAATTTTG+AGG + chr4.3:67050530-67050549 MS.gene88261:intron 20.0%
! AAAGAAGCAATTTCAAGAAA+AGG + chr4.3:67050779-67050798 MS.gene88261:CDS 25.0%
! AAGAAAGAGAAAAGAAAGAA+TGG + chr4.3:67050918-67050937 MS.gene88261:intron 25.0%
! AAGAAGCAATTTCAAGAAAA+GGG + chr4.3:67050780-67050799 MS.gene88261:CDS 25.0%
! AATCAAATGTTGGTACATTT+AGG + chr4.3:67050588-67050607 MS.gene88261:intron 25.0%
! ACTCAAAATTTGAACTTTGT+TGG + chr4.3:67049802-67049821 MS.gene88261:CDS 25.0%
! ACTGTTCTTCAATATCTTTA+AGG - chr4.3:67050759-67050778 None:intergenic 25.0%
! ATCAAATGTTGGTACATTTA+GGG + chr4.3:67050589-67050608 MS.gene88261:intron 25.0%
! ATTATGAAATCAGAACATAC+AGG - chr4.3:67049927-67049946 None:intergenic 25.0%
! ATTTCAAGAAAAGGGAAAAA+AGG + chr4.3:67050788-67050807 MS.gene88261:CDS 25.0%
! CAAAAAAAAATTACTTGTGG+GGG + chr4.3:67049965-67049984 MS.gene88261:intron 25.0%
! CCAAAAAAAAATTACTTGTG+GGG + chr4.3:67049964-67049983 MS.gene88261:intron 25.0%
! TTATGAAATCAGAACATACA+GGG - chr4.3:67049926-67049945 None:intergenic 25.0%
!!! AAGCCTAAGAAATATTTTAG+AGG + chr4.3:67050559-67050578 MS.gene88261:intron 25.0%
!!! AGCCTAAGAAATATTTTAGA+GGG + chr4.3:67050560-67050579 MS.gene88261:intron 25.0%
!!! ATAAGTTTTGACTAAAACGT+AGG + chr4.3:67050500-67050519 MS.gene88261:intron 25.0%
!!! CATAATTTTTAGCAATGTGT+AGG + chr4.3:67050015-67050034 MS.gene88261:intron 25.0%
!!! GATAAACTTAGTTTGTTTCA+AGG + chr4.3:67049589-67049608 MS.gene88261:CDS 25.0%
!!! TAAACTTAGTTTGTTTCAAG+GGG + chr4.3:67049591-67049610 MS.gene88261:CDS 25.0%
!!! TAAGTTTTGACTAAAACGTA+GGG + chr4.3:67050501-67050520 MS.gene88261:intron 25.0%
AAGAAACTCATATATGATGC+AGG + chr4.3:67050941-67050960 MS.gene88261:intron 30.0%
ATCTCAAATGTCTTTCAAAG+TGG + chr4.3:67050705-67050724 MS.gene88261:intron 30.0%
CAAAATTTGAACTTTGTTGG+TGG + chr4.3:67049805-67049824 MS.gene88261:CDS 30.0%
CATACAGGGACAAAATAAAA+TGG - chr4.3:67049912-67049931 None:intergenic 30.0%
CATACATAGAGAGATATTTC+CGG - chr4.3:67050245-67050264 None:intergenic 30.0%
CTAAAGTTAGTGTCCTATAT+AGG + chr4.3:67049299-67049318 MS.gene88261:CDS 30.0%
TATAAGATTACTCCCATATG+CGG + chr4.3:67050974-67050993 MS.gene88261:CDS 30.0%
TATAGGACACTAACTTTAGA+AGG - chr4.3:67049298-67049317 None:intergenic 30.0%
TCAAATGTTGGTACATTTAG+GGG + chr4.3:67050590-67050609 MS.gene88261:intron 30.0%
TCTCAAATGTCTTTCAAAGT+GGG + chr4.3:67050706-67050725 MS.gene88261:intron 30.0%
TTTATATCTTTGATCAGGCA+CGG - chr4.3:67050293-67050312 None:intergenic 30.0%
! ATACAGCTTTATAAGTTCCA+TGG + chr4.3:67049174-67049193 MS.gene88261:CDS 30.0%
! ATAGGACACTAACTTTAGAA+GGG - chr4.3:67049297-67049316 None:intergenic 30.0%
! CTTTGGGTTAAATACGTAAA+TGG - chr4.3:67050618-67050637 None:intergenic 30.0%
!! GTCCCTCTAAAATATTTCTT+AGG - chr4.3:67050565-67050584 None:intergenic 30.0%
!!! CCCCACAAGTAATTTTTTTT+TGG - chr4.3:67049967-67049986 None:intergenic 30.0%
!!! TTGCTGATTTTTAACACTAC+CGG + chr4.3:67050223-67050242 MS.gene88261:intron 30.0%
AAAACTGTGCATTTGATGTG+AGG - chr4.3:67049748-67049767 None:intergenic 35.0%
CAAATGTTGGTACATTTAGG+GGG + chr4.3:67050591-67050610 MS.gene88261:intron 35.0%
GAGGGACTAAAATCAAATGT+TGG + chr4.3:67050578-67050597 MS.gene88261:intron 35.0%
GTAATGATGACTCTGCATAA+TGG + chr4.3:67050893-67050912 MS.gene88261:intron 35.0%
TTCAAAGATGAGGCTAATTC+AGG - chr4.3:67049268-67049287 None:intergenic 35.0%
! TGCACTTTATGAAAGTGCAA+CGG + chr4.3:67049525-67049544 MS.gene88261:CDS 35.0%
!! AAAGTGGGTTTTGCATGATT+GGG + chr4.3:67050721-67050740 MS.gene88261:intron 35.0%
ACTAACAAGTGGTGCAAAAC+AGG - chr4.3:67049434-67049453 None:intergenic 40.0%
AGGGTGAAGTTTCAAAGATG+AGG - chr4.3:67049278-67049297 None:intergenic 40.0%
ATCCACTCAGATGAGATTTG+GGG - chr4.3:67050270-67050289 None:intergenic 40.0%
ATCCATGTTCTCTGGATATG+TGG + chr4.3:67049464-67049483 MS.gene88261:CDS 40.0%
CTCAGAGAACCATTTCTTAG+AGG - chr4.3:67049494-67049513 None:intergenic 40.0%
GATCCACTCAGATGAGATTT+GGG - chr4.3:67050271-67050290 None:intergenic 40.0%
GCTAATTCAGGAACAGTCAT+TGG - chr4.3:67049256-67049275 None:intergenic 40.0%
GTAGATGCAATCCACAAACA+TGG + chr4.3:67049226-67049245 MS.gene88261:CDS 40.0%
GTCAAAGTGCAATTGCAACT+CGG - chr4.3:67050870-67050889 None:intergenic 40.0%
GTTGCAATTGCACTTTGACA+TGG + chr4.3:67050871-67050890 MS.gene88261:CDS 40.0%
TTGAGTAAAACAGCATCTGC+AGG - chr4.3:67049853-67049872 None:intergenic 40.0%
TTTAGATCAACCACAGGTTG+TGG + chr4.3:67049765-67049784 MS.gene88261:CDS 40.0%
! AATGTTCAAGCTTGCTCTCA+AGG + chr4.3:67049633-67049652 MS.gene88261:CDS 40.0%
! ACTTTATGAAAGTGCAACGG+GGG + chr4.3:67049528-67049547 MS.gene88261:CDS 40.0%
! CACTTTATGAAAGTGCAACG+GGG + chr4.3:67049527-67049546 MS.gene88261:CDS 40.0%
! CTCTAAGAAATGGTTCTCTG+AGG + chr4.3:67049492-67049511 MS.gene88261:CDS 40.0%
! GCACTTTATGAAAGTGCAAC+GGG + chr4.3:67049526-67049545 MS.gene88261:CDS 40.0%
! TTTGGAGTCTTTGGTTGATG+TGG + chr4.3:67049678-67049697 MS.gene88261:CDS 40.0%
!! CAAAGTGGGTTTTGCATGAT+TGG + chr4.3:67050720-67050739 MS.gene88261:intron 40.0%
!! TCATTGGCTTTCCATGTTTG+TGG - chr4.3:67049240-67049259 None:intergenic 40.0%
!! TGTTTTGCACCACTTGTTAG+TGG + chr4.3:67049433-67049452 MS.gene88261:CDS 40.0%
!!! CACAGTTTTAGATCAACCAC+AGG + chr4.3:67049759-67049778 MS.gene88261:CDS 40.0%
!!! TGCAGATGCTGTTTTACTCA+AGG + chr4.3:67049852-67049871 MS.gene88261:CDS 40.0%
ACTCCAAACCCTCAAACACA+TGG - chr4.3:67049666-67049685 None:intergenic 45.0%
AGAGACTTGAAGCCGCATAT+GGG - chr4.3:67050989-67051008 None:intergenic 45.0%
AGTAAAACAGCATCTGCAGG+AGG - chr4.3:67049850-67049869 None:intergenic 45.0%
AGTGCTTCATCCATGTTCTC+TGG + chr4.3:67049456-67049475 MS.gene88261:CDS 45.0%
CAGGTTGTGGCTAACTTATC+TGG + chr4.3:67049778-67049797 MS.gene88261:CDS 45.0%
CATTTGTGAATCAGCTGCCA+TGG - chr4.3:67049617-67049636 None:intergenic 45.0%
CCTTCAAAGCTTCTCATCAG+TGG + chr4.3:67049400-67049419 MS.gene88261:CDS 45.0%
CTCAAATCAGCTGTGGAACT+AGG + chr4.3:67049199-67049218 MS.gene88261:CDS 45.0%
GATAAGTTAGCCACAACCTG+TGG - chr4.3:67049778-67049797 None:intergenic 45.0%
GATTGCAGCCATGTGTTTGA+GGG + chr4.3:67049655-67049674 MS.gene88261:CDS 45.0%
GCAAGCGCAAGAACCTATAT+AGG - chr4.3:67049315-67049334 None:intergenic 45.0%
GGATCCACTCAGATGAGATT+TGG - chr4.3:67050272-67050291 None:intergenic 45.0%
GTAAAACAGCATCTGCAGGA+GGG - chr4.3:67049849-67049868 None:intergenic 45.0%
TGCCACATATCCAGAGAACA+TGG - chr4.3:67049469-67049488 None:intergenic 45.0%
! CCACTGATGAGAAGCTTTGA+AGG - chr4.3:67049403-67049422 None:intergenic 45.0%
! GAAATGGTTCTCTGAGGAGA+AGG + chr4.3:67049498-67049517 MS.gene88261:CDS 45.0%
! GTTTGAGGGTTTGGAGTCTT+TGG + chr4.3:67049669-67049688 MS.gene88261:CDS 45.0%
! TGAAGCACTCCACTAACAAG+TGG - chr4.3:67049445-67049464 None:intergenic 45.0%
! TGTGGCACTCCTCTAAGAAA+TGG + chr4.3:67049482-67049501 MS.gene88261:CDS 45.0%
!! CTGATGAGAAGCTTTGAAGG+AGG - chr4.3:67049400-67049419 None:intergenic 45.0%
!! TAGTTTGTTTCAAGGGGCCA+TGG + chr4.3:67049597-67049616 MS.gene88261:CDS 45.0%
!!! ATTAATTTTTCTAAATATAA+TGG - chr4.3:67050132-67050151 None:intergenic 5.0%
CAGCCATGTGTTTGAGGGTT+TGG + chr4.3:67049660-67049679 MS.gene88261:CDS 50.0%
CATGGCACTCAAATCAGCTG+TGG + chr4.3:67049192-67049211 MS.gene88261:CDS 50.0%
GAGAGACTTGAAGCCGCATA+TGG - chr4.3:67050990-67051009 None:intergenic 50.0%
GGATTGCAGCCATGTGTTTG+AGG + chr4.3:67049654-67049673 MS.gene88261:CDS 50.0%
TGCCCCAAATCTCATCTGAG+TGG + chr4.3:67050265-67050284 MS.gene88261:intron 50.0%
! CTTTGGTTGATGTGGCAGGT+GGG + chr4.3:67049686-67049705 MS.gene88261:CDS 50.0%
! GAGTCTTTGGTTGATGTGGC+AGG + chr4.3:67049682-67049701 MS.gene88261:CDS 50.0%
! TCTTTGGTTGATGTGGCAGG+TGG + chr4.3:67049685-67049704 MS.gene88261:CDS 50.0%
! TTGCGCTTGCTAACACACGA+TGG + chr4.3:67049325-67049344 MS.gene88261:CDS 50.0%
!! CACAGCTGATTTGAGTGCCA+TGG - chr4.3:67049194-67049213 None:intergenic 50.0%
GTTGATGTGGCAGGTGGGAC+TGG + chr4.3:67049691-67049710 MS.gene88261:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 67049103 67051013 67049103 ID=MS.gene88261
chr4.3 mRNA 67049103 67051013 67049103 ID=MS.gene88261.t1;Parent=MS.gene88261
chr4.3 exon 67049103 67049873 67049103 ID=MS.gene88261.t1.exon1;Parent=MS.gene88261.t1
chr4.3 CDS 67049103 67049873 67049103 ID=cds.MS.gene88261.t1;Parent=MS.gene88261.t1
chr4.3 exon 67050725 67050892 67050725 ID=MS.gene88261.t1.exon2;Parent=MS.gene88261.t1
chr4.3 CDS 67050725 67050892 67050725 ID=cds.MS.gene88261.t1;Parent=MS.gene88261.t1
chr4.3 exon 67050963 67051013 67050963 ID=MS.gene88261.t1.exon3;Parent=MS.gene88261.t1
chr4.3 CDS 67050963 67051013 67050963 ID=cds.MS.gene88261.t1;Parent=MS.gene88261.t1
Gene Sequence

>MS.gene88261

ATGGATTTCAAGAGCAATGGCAGTGAAGAAACTCAGCTGTATCATGCTCAAATCCATTTATACAAACATGTATACAGCTTTATAAGTTCCATGGCACTCAAATCAGCTGTGGAACTAGGCATAGTAGATGCAATCCACAAACATGGAAAGCCAATGACTGTTCCTGAATTAGCCTCATCTTTGAAACTTCACCCTTCTAAAGTTAGTGTCCTATATAGGTTCTTGCGCTTGCTAACACACGATGGTTTCTTTGCAAAAACAACAGTGAAAGAAGAAACAGCTTATGCTCTCACACCTCCTTCAAAGCTTCTCATCAGTGGTGAATCAACCTGTTTTGCACCACTTGTTAGTGGAGTGCTTCATCCATGTTCTCTGGATATGTGGCACTCCTCTAAGAAATGGTTCTCTGAGGAGAAGGATCTTGCACTTTATGAAAGTGCAACGGGGGAGACCTTTTTTAACTTTCTTAACAAAGATTCTGAATCTGATAAACTTAGTTTGTTTCAAGGGGCCATGGCAGCTGATTCACAAATGTTCAAGCTTGCTCTCAAGGATTGCAGCCATGTGTTTGAGGGTTTGGAGTCTTTGGTTGATGTGGCAGGTGGGACTGGAGTTGTGTCAAAACTCATTCATGAAGAATTTCCTCACATCAAATGCACAGTTTTAGATCAACCACAGGTTGTGGCTAACTTATCTGGAACTCAAAATTTGAACTTTGTTGGTGGAGATATGTTCAAATCTATCCCTCCTGCAGATGCTGTTTTACTCAAGGTACGTTTATGTTTATCAATTTTTACCATTTGATTCCATTTTATTTTGTCCCTGTATGTTCTGATTTCATAATTAGGATTAGTGACAAATCCAAAAAAAAATTACTTGTGGGGGCAAGATTTAATATAATAAATATTTATACATAATTTTTAGCAATGTGTAGGTGATAAAATAATAACATCGGTTTTCTTTTTACTACTAATAAATAATAACATCATTATCTTTGAAAAATATACTTGAGATTTTAAACTCACACCATTATATTTAGAAAAATTAATGATTTTTTAAGATCTCCAAATAAAAATTGTTCTTACGTGTCTTTATATGATTAAAAAAAGTATTGTAAATTTTGCTGATTTTTAACACTACCGGAAATATCTCTCTATGTATGTGCCCCAAATCTCATCTGAGTGGATCCGTGCCTGATCAAAGATATAAATTTGTGGTAACTTTTTTAGTTAAGATATTCCAATGAATTAAATATGAATTTTTATGTTTTTGGTGTTAAAAAATTCATGTTATATTTATCTTTTGTTAAAAAATTCTATTTCTTTTTTAGAAAAATATTGTCATAATTTTCTACACATTTCTTTAAAATTTCATTAAAAAAAATATGAAGGTTAGACATAAGTTTTGACTAAAACGTAGGGACTAATTTCATAGATGAAAAATTTTGAGGGATCGAAAGCCTAAGAAATATTTTAGAGGGACTAAAATCAAATGTTGGTACATTTAGGGGGACCATTTACGTATTTAACCCAAAGAAATAAAATTGTAAAATAATATTATTTTAATTTATTGGTCTATCTCATTTTGTAACATTGTACTAAAATCTCAAATGTCTTTCAAAGTGGGTTTTGCATGATTGGGATGATGAGCTCTCCTTAAAGATATTGAAGAACAGTAAAGAAGCAATTTCAAGAAAAGGGAAAAAAGGAAAGATAATAATCATAGACATATCAATTGATGAAACAAGTGATGATCGTGAGTTAACCGAGTTGCAATTGCACTTTGACATGGTAATGATGACTCTGCATAATGGAAAAGAAAGAGAAAAGAAAGAATGGAAGAAACTCATATATGATGCAGGCTTCAGCAGCTATAAGATTACTCCCATATGCGGCTTCAAGTCTCTCATAG
Protein sequence

>MS.gene88261.t1

MDFKSNGSEETQLYHAQIHLYKHVYSFISSMALKSAVELGIVDAIHKHGKPMTVPELASSLKLHPSKVSVLYRFLRLLTHDGFFAKTTVKEETAYALTPPSKLLISGESTCFAPLVSGVLHPCSLDMWHSSKKWFSEEKDLALYESATGETFFNFLNKDSESDKLSLFQGAMAADSQMFKLALKDCSHVFEGLESLVDVAGGTGVVSKLIHEEFPHIKCTVLDQPQVVANLSGTQNLNFVGGDMFKSIPPADAVLLKWVLHDWDDELSLKILKNSKEAISRKGKKGKIIIIDISIDETSDDRELTELQLHFDMASAAIRLLPYAASSLS