Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88384.t1 | XP_013464784.1 | 96.6 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 2.50E-39 | 171 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88384.t1 | A0A072V9V8 | 96.6 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 1.8e-39 | 171.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053108 | MS.gene88384 | 0.836787 | 7.63E-57 | -1.69E-46 |
| MS.gene053421 | MS.gene88384 | 0.823319 | 1.48E-53 | -1.69E-46 |
| MS.gene056618 | MS.gene88384 | 0.815917 | 7.23E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88384.t1 | MTR_2g481150 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 1.93e-60 | 179 |
| MS.gene88384.t1 | MTR_2g481160 | 93.258 | 89 | 6 | 0 | 1 | 89 | 1 | 89 | 9.80e-53 | 159 |
| MS.gene88384.t1 | MTR_2g081155 | 74.725 | 91 | 17 | 4 | 1 | 89 | 1 | 87 | 7.87e-41 | 129 |
| MS.gene88384.t1 | MTR_4g045990 | 56.180 | 89 | 35 | 1 | 1 | 85 | 1 | 89 | 3.72e-31 | 105 |
| MS.gene88384.t1 | MTR_2g089945 | 53.933 | 89 | 34 | 2 | 2 | 85 | 5 | 91 | 5.14e-28 | 97.4 |
| MS.gene88384.t1 | MTR_2g089905 | 55.914 | 93 | 32 | 3 | 1 | 85 | 1 | 92 | 1.97e-27 | 95.9 |
| MS.gene88384.t1 | MTR_2g089930 | 51.724 | 87 | 39 | 1 | 2 | 85 | 5 | 91 | 1.18e-26 | 94.0 |
| MS.gene88384.t1 | MTR_2g089940 | 52.174 | 92 | 36 | 3 | 1 | 85 | 1 | 91 | 4.43e-26 | 92.4 |
| MS.gene88384.t1 | MTR_2g089985 | 52.174 | 92 | 36 | 3 | 1 | 85 | 1 | 91 | 4.43e-26 | 92.4 |
| MS.gene88384.t1 | MTR_2g089960 | 52.174 | 92 | 36 | 3 | 1 | 85 | 1 | 91 | 4.43e-26 | 92.4 |
| MS.gene88384.t1 | MTR_2g089970 | 51.685 | 89 | 37 | 3 | 1 | 84 | 1 | 88 | 5.12e-25 | 89.7 |
| MS.gene88384.t1 | MTR_2g089990 | 50.562 | 89 | 38 | 3 | 1 | 84 | 1 | 88 | 4.98e-24 | 87.0 |
| MS.gene88384.t1 | MTR_2g089965 | 52.577 | 97 | 33 | 3 | 1 | 85 | 1 | 96 | 5.09e-24 | 87.4 |
| MS.gene88384.t1 | MTR_2g089950 | 50.000 | 86 | 38 | 3 | 1 | 84 | 1 | 83 | 5.16e-22 | 82.0 |
| MS.gene88384.t1 | MTR_2g089865 | 54.321 | 81 | 30 | 2 | 2 | 77 | 5 | 83 | 8.01e-21 | 84.0 |
| MS.gene88384.t1 | MTR_2g089925 | 46.512 | 86 | 41 | 3 | 1 | 84 | 1 | 83 | 4.28e-20 | 77.0 |
| MS.gene88384.t1 | MTR_2g089835 | 42.697 | 89 | 46 | 3 | 1 | 85 | 1 | 88 | 1.64e-15 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88384.t1 | AT4G10265 | 65.882 | 85 | 27 | 2 | 1 | 85 | 1 | 83 | 3.29e-34 | 112 |
| MS.gene88384.t1 | AT4G10270 | 53.333 | 90 | 37 | 1 | 1 | 85 | 1 | 90 | 1.10e-27 | 96.7 |
| MS.gene88384.t1 | AT4G33560 | 40.625 | 96 | 49 | 3 | 1 | 89 | 1 | 95 | 3.89e-15 | 64.7 |
Find 22 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGTCCATGACTTTCTTCA+TGG | 0.260032 | 2.4:-17193114 | None:intergenic |
| CTTGGTTCTCTTGACTTTGT+TGG | 0.278878 | 2.4:-17193084 | None:intergenic |
| AGAAGCAGATGACAATTTCT+TGG | 0.287978 | 2.4:-17193048 | None:intergenic |
| GTATGATCTGATATTGTTCT+TGG | 0.335862 | 2.4:-17193018 | None:intergenic |
| AAGACCAACTTGGTGTTTGC+AGG | 0.345102 | 2.4:+17192961 | MS.gene88384:CDS |
| TTTCTTCATGGACTCTTCCT+TGG | 0.358781 | 2.4:-17193102 | None:intergenic |
| CATTAAAACCTTGCAGTACT+AGG | 0.414275 | 2.4:-17193149 | None:intergenic |
| AAGTCATGGACTTGAATTGC+TGG | 0.419101 | 2.4:+17193123 | MS.gene88384:CDS |
| TGAGTGCAGCAACAAAAGCT+TGG | 0.493392 | 2.4:+17192901 | MS.gene88384:CDS |
| GAGTGCAGCAACAAAAGCTT+GGG | 0.515342 | 2.4:+17192902 | MS.gene88384:CDS |
| AGCATCAAGCATAGGAGCAG+TGG | 0.536575 | 2.4:+17192929 | MS.gene88384:CDS |
| AGTCATGGACTTGAATTGCT+GGG | 0.546309 | 2.4:+17193124 | MS.gene88384:CDS |
| ATCAAGCATAGGAGCAGTGG+AGG | 0.556011 | 2.4:+17192932 | MS.gene88384:CDS |
| ACCAACTTGGTGTTTGCAGG+TGG | 0.578723 | 2.4:+17192964 | MS.gene88384:CDS |
| TGGGTAGTAGCATCAAGCAT+AGG | 0.606039 | 2.4:+17192921 | MS.gene88384:CDS |
| GCTGGGGTCCTAGTACTGCA+AGG | 0.615045 | 2.4:+17193141 | MS.gene88384:CDS |
| TCCACCTGCAAACACCAAGT+TGG | 0.616927 | 2.4:-17192965 | None:intergenic |
| AGAGTCCATGAAGAAAGTCA+TGG | 0.627949 | 2.4:+17193109 | MS.gene88384:CDS |
| GTCATGGACTTGAATTGCTG+GGG | 0.644611 | 2.4:+17193125 | MS.gene88384:CDS |
| GAGGCCTTAAAAGACCAACT+TGG | 0.665081 | 2.4:+17192951 | MS.gene88384:CDS |
| GATGCAGTGATCTAAAAGCA+TGG | 0.715842 | 2.4:-17192989 | None:intergenic |
| CAACAAAGTCAAGAGAACCA+AGG | 0.721950 | 2.4:+17193085 | MS.gene88384:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTATGATCTGATATTGTTCT+TGG | - | chr2.4:17193021-17193040 | None:intergenic | 30.0% | |
| AGAAGCAGATGACAATTTCT+TGG | - | chr2.4:17193051-17193070 | None:intergenic | 35.0% | |
| !!! | AACACCAAGTTGGTCTTTTA+AGG | - | chr2.4:17192958-17192977 | None:intergenic | 35.0% |
| AAGTCATGGACTTGAATTGC+TGG | + | chr2.4:17193123-17193142 | MS.gene88384:CDS | 40.0% | |
| AGAGTCCATGAAGAAAGTCA+TGG | + | chr2.4:17193109-17193128 | MS.gene88384:CDS | 40.0% | |
| AGTCATGGACTTGAATTGCT+GGG | + | chr2.4:17193124-17193143 | MS.gene88384:CDS | 40.0% | |
| CAACAAAGTCAAGAGAACCA+AGG | + | chr2.4:17193085-17193104 | MS.gene88384:CDS | 40.0% | |
| CAAGTCCATGACTTTCTTCA+TGG | - | chr2.4:17193117-17193136 | None:intergenic | 40.0% | |
| GATGCAGTGATCTAAAAGCA+TGG | - | chr2.4:17192992-17193011 | None:intergenic | 40.0% | |
| TTTCTTCATGGACTCTTCCT+TGG | - | chr2.4:17193105-17193124 | None:intergenic | 40.0% | |
| ! | CTTGGTTCTCTTGACTTTGT+TGG | - | chr2.4:17193087-17193106 | None:intergenic | 40.0% |
| GAGGCCTTAAAAGACCAACT+TGG | + | chr2.4:17192951-17192970 | MS.gene88384:CDS | 45.0% | |
| GAGTGCAGCAACAAAAGCTT+GGG | + | chr2.4:17192902-17192921 | MS.gene88384:CDS | 45.0% | |
| GTCATGGACTTGAATTGCTG+GGG | + | chr2.4:17193125-17193144 | MS.gene88384:CDS | 45.0% | |
| TGAGTGCAGCAACAAAAGCT+TGG | + | chr2.4:17192901-17192920 | MS.gene88384:CDS | 45.0% | |
| TGGGTAGTAGCATCAAGCAT+AGG | + | chr2.4:17192921-17192940 | MS.gene88384:CDS | 45.0% | |
| !! | AAGACCAACTTGGTGTTTGC+AGG | + | chr2.4:17192961-17192980 | MS.gene88384:CDS | 45.0% |
| AGCATCAAGCATAGGAGCAG+TGG | + | chr2.4:17192929-17192948 | MS.gene88384:CDS | 50.0% | |
| ATCAAGCATAGGAGCAGTGG+AGG | + | chr2.4:17192932-17192951 | MS.gene88384:CDS | 50.0% | |
| TCCACCTGCAAACACCAAGT+TGG | - | chr2.4:17192968-17192987 | None:intergenic | 50.0% | |
| !! | ACCAACTTGGTGTTTGCAGG+TGG | + | chr2.4:17192964-17192983 | MS.gene88384:CDS | 50.0% |
| GCTGGGGTCCTAGTACTGCA+AGG | + | chr2.4:17193141-17193160 | MS.gene88384:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 17192900 | 17193169 | 17192900 | ID=MS.gene88384 |
| chr2.4 | mRNA | 17192900 | 17193169 | 17192900 | ID=MS.gene88384.t1;Parent=MS.gene88384 |
| chr2.4 | exon | 17192900 | 17193169 | 17192900 | ID=MS.gene88384.t1.exon1;Parent=MS.gene88384.t1 |
| chr2.4 | CDS | 17192900 | 17193169 | 17192900 | ID=cds.MS.gene88384.t1;Parent=MS.gene88384.t1 |
| Gene Sequence |
| Protein sequence |
