Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88643.t1 | XP_003610102.1 | 96 | 101 | 4 | 0 | 2 | 102 | 4 | 104 | 3.70E-47 | 197.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88643.t1 | Q9ZQ19 | 52.0 | 102 | 48 | 1 | 2 | 102 | 5 | 106 | 1.4e-23 | 110.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88643.t1 | A0A072UTZ7 | 96.0 | 101 | 4 | 0 | 2 | 102 | 4 | 104 | 2.7e-47 | 197.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049989 | MS.gene88643 | 0.812798 | 3.54E-51 | -1.69E-46 |
| MS.gene053173 | MS.gene88643 | 0.820722 | 5.90E-53 | -1.69E-46 |
| MS.gene053348 | MS.gene88643 | 0.841255 | 5.31E-58 | -1.69E-46 |
| MS.gene054551 | MS.gene88643 | 0.838962 | 2.10E-57 | -1.69E-46 |
| MS.gene054593 | MS.gene88643 | 0.825946 | 3.55E-54 | -1.69E-46 |
| MS.gene056526 | MS.gene88643 | 0.804237 | 2.38E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88643.t1 | MTR_4g127950 | 96.040 | 101 | 4 | 0 | 2 | 102 | 4 | 104 | 1.91e-67 | 202 |
| MS.gene88643.t1 | MTR_4g127950 | 96.040 | 101 | 4 | 0 | 2 | 102 | 4 | 104 | 1.39e-66 | 201 |
| MS.gene88643.t1 | MTR_1g009890 | 52.475 | 101 | 45 | 1 | 2 | 102 | 5 | 102 | 9.07e-30 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88643.t1 | AT2G24490 | 51.961 | 102 | 48 | 1 | 2 | 102 | 5 | 106 | 7.22e-32 | 113 |
| MS.gene88643.t1 | AT2G24490 | 51.961 | 102 | 48 | 1 | 2 | 102 | 5 | 106 | 7.22e-32 | 113 |
| MS.gene88643.t1 | AT3G02920 | 50.505 | 99 | 47 | 1 | 4 | 102 | 6 | 102 | 1.25e-29 | 105 |
| MS.gene88643.t1 | AT3G02920 | 50.505 | 99 | 47 | 1 | 4 | 102 | 6 | 102 | 1.25e-29 | 105 |
| MS.gene88643.t1 | AT3G02920 | 50.505 | 99 | 47 | 1 | 4 | 102 | 6 | 102 | 3.21e-29 | 106 |
Find 29 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAGGGTTTGCAACAAATC+TGG | 0.360046 | 8.4:+6551101 | MS.gene88643:CDS |
| TCTGTCCAGGTTACTCTTGT+TGG | 0.363119 | 8.4:+6551080 | MS.gene88643:intron |
| GAACAATCGAGTGTACTAAA+TGG | 0.372337 | 8.4:+6551165 | MS.gene88643:CDS |
| GACGGTAACGCCGCTTTCTC+CGG | 0.395800 | 8.4:+6550677 | MS.gene88643:CDS |
| CGGCGGTTTCATGCCTTCTC+AGG | 0.416064 | 8.4:+6550700 | MS.gene88643:CDS |
| CCAGGTTACTCTTGTTGGTA+GGG | 0.423374 | 8.4:+6551085 | MS.gene88643:intron |
| AGAGGATTCACCGCCTTGAT+TGG | 0.430570 | 8.4:-6550723 | None:intergenic |
| TCCAGGTTACTCTTGTTGGT+AGG | 0.433239 | 8.4:+6551084 | MS.gene88643:intron |
| ATGCCTTCTCAGGCCAATCA+AGG | 0.473622 | 8.4:+6550710 | MS.gene88643:CDS |
| AACGCCGCTTTCTCCGGCGG+CGG | 0.487082 | 8.4:+6550683 | MS.gene88643:CDS |
| TTTGTACTTGACGATGGCAC+TGG | 0.500314 | 8.4:+6551143 | MS.gene88643:CDS |
| CCGCCTTGATTGGCCTGAGA+AGG | 0.504121 | 8.4:-6550713 | None:intergenic |
| CAATCAAGTGATGATAGAAC+CGG | 0.507029 | 8.4:+6550961 | MS.gene88643:CDS |
| ATCCTCTCTCACTCCCTCCA+AGG | 0.534425 | 8.4:+6550739 | MS.gene88643:CDS |
| AACCTTGGAGGGAGTGAGAG+AGG | 0.538834 | 8.4:-6550741 | None:intergenic |
| GAAACCGCCGCCGGAGAAAG+CGG | 0.554313 | 8.4:-6550687 | None:intergenic |
| GGTCTTACTATTGACGGTGT+TGG | 0.564776 | 8.4:+6550982 | MS.gene88643:CDS |
| CATCACTTGATTGAAGAGCA+TGG | 0.578483 | 8.4:-6550951 | None:intergenic |
| AACACCGTCAATAGTAAGAC+CGG | 0.588874 | 8.4:-6550980 | None:intergenic |
| AGAACTGAGAAGAACCTTGG+AGG | 0.589879 | 8.4:-6550753 | None:intergenic |
| TTTAAGTTTGTACTTGACGA+TGG | 0.593316 | 8.4:+6551137 | MS.gene88643:CDS |
| AGAACCGGTCTTACTATTGA+CGG | 0.593890 | 8.4:+6550976 | MS.gene88643:CDS |
| GGTAACGCCGCTTTCTCCGG+CGG | 0.597825 | 8.4:+6550680 | MS.gene88643:CDS |
| GAACTGAGAAGAACCTTGGA+GGG | 0.608680 | 8.4:-6550752 | None:intergenic |
| GTTACCGTCGAACTGACTCA+TGG | 0.626404 | 8.4:-6550663 | None:intergenic |
| AGAAGGCATGAAACCGCCGC+CGG | 0.632650 | 8.4:-6550696 | None:intergenic |
| TTGATCTGCTTGATCGTCAG+CGG | 0.639696 | 8.4:-6550928 | None:intergenic |
| GGCACCATGAGTCAGTTCGA+CGG | 0.714184 | 8.4:+6550659 | None:intergenic |
| CCTTCTCAGGCCAATCAAGG+CGG | 0.735731 | 8.4:+6550713 | MS.gene88643:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAGATTATTAAGAAGAAAA+TGG | - | chr8.4:6550785-6550804 | None:intergenic | 15.0% |
| !! | CATGTAATTGATGTGTTTTA+GGG | + | chr8.4:6550861-6550880 | MS.gene88643:intron | 25.0% |
| !! | TCATGTAATTGATGTGTTTT+AGG | + | chr8.4:6550860-6550879 | MS.gene88643:intron | 25.0% |
| AACATCAATTAGACATGCTA+GGG | + | chr8.4:6550810-6550829 | MS.gene88643:intron | 30.0% | |
| CTATTGTGAATTACGATTTG+TGG | + | chr8.4:6551033-6551052 | MS.gene88643:intron | 30.0% | |
| ! | TTTAAGTTTGTACTTGACGA+TGG | + | chr8.4:6551137-6551156 | MS.gene88643:CDS | 30.0% |
| AATTAGACATGCTAGGGTTA+GGG | + | chr8.4:6550816-6550835 | MS.gene88643:intron | 35.0% | |
| AGCATGTCTAATTGATGTTG+CGG | - | chr8.4:6550809-6550828 | None:intergenic | 35.0% | |
| CAACATCAATTAGACATGCT+AGG | + | chr8.4:6550809-6550828 | MS.gene88643:intron | 35.0% | |
| CAATCAAGTGATGATAGAAC+CGG | + | chr8.4:6550961-6550980 | MS.gene88643:CDS | 35.0% | |
| CTGGACAGAATAAAATTGTG+AGG | - | chr8.4:6551069-6551088 | None:intergenic | 35.0% | |
| GAACAATCGAGTGTACTAAA+TGG | + | chr8.4:6551165-6551184 | MS.gene88643:CDS | 35.0% | |
| ! | CTCACAATTTTATTCTGTCC+AGG | + | chr8.4:6551067-6551086 | MS.gene88643:intron | 35.0% |
| AACACCGTCAATAGTAAGAC+CGG | - | chr8.4:6550983-6551002 | None:intergenic | 40.0% | |
| AGAACCGGTCTTACTATTGA+CGG | + | chr8.4:6550976-6550995 | MS.gene88643:CDS | 40.0% | |
| CAATTAGACATGCTAGGGTT+AGG | + | chr8.4:6550815-6550834 | MS.gene88643:intron | 40.0% | |
| CATCACTTGATTGAAGAGCA+TGG | - | chr8.4:6550954-6550973 | None:intergenic | 40.0% | |
| ACATGCTAGGGTTAGGGTTT+AGG | + | chr8.4:6550822-6550841 | MS.gene88643:intron | 45.0% | |
| AGAACTGAGAAGAACCTTGG+AGG | - | chr8.4:6550756-6550775 | None:intergenic | 45.0% | |
| CATGCTAGGGTTAGGGTTTA+GGG | + | chr8.4:6550823-6550842 | MS.gene88643:intron | 45.0% | |
| CCAGGTTACTCTTGTTGGTA+GGG | + | chr8.4:6551085-6551104 | MS.gene88643:intron | 45.0% | |
| GAACTGAGAAGAACCTTGGA+GGG | - | chr8.4:6550755-6550774 | None:intergenic | 45.0% | |
| GGTAGGGTTTGCAACAAATC+TGG | + | chr8.4:6551101-6551120 | MS.gene88643:CDS | 45.0% | |
| GGTCTTACTATTGACGGTGT+TGG | + | chr8.4:6550982-6551001 | MS.gene88643:CDS | 45.0% | |
| GTGAGAACTGAGAAGAACCT+TGG | - | chr8.4:6550759-6550778 | None:intergenic | 45.0% | |
| TCCAGGTTACTCTTGTTGGT+AGG | + | chr8.4:6551084-6551103 | MS.gene88643:intron | 45.0% | |
| TCTGTCCAGGTTACTCTTGT+TGG | + | chr8.4:6551080-6551099 | MS.gene88643:intron | 45.0% | |
| TTGATCTGCTTGATCGTCAG+CGG | - | chr8.4:6550931-6550950 | None:intergenic | 45.0% | |
| TTTGTACTTGACGATGGCAC+TGG | + | chr8.4:6551143-6551162 | MS.gene88643:CDS | 45.0% | |
| AGAGGATTCACCGCCTTGAT+TGG | - | chr8.4:6550726-6550745 | None:intergenic | 50.0% | |
| ATGCCTTCTCAGGCCAATCA+AGG | + | chr8.4:6550710-6550729 | MS.gene88643:CDS | 50.0% | |
| CCCTACCAACAAGAGTAACC+TGG | - | chr8.4:6551088-6551107 | None:intergenic | 50.0% | |
| AACCTTGGAGGGAGTGAGAG+AGG | - | chr8.4:6550744-6550763 | None:intergenic | 55.0% | |
| ATCCTCTCTCACTCCCTCCA+AGG | + | chr8.4:6550739-6550758 | MS.gene88643:CDS | 55.0% | |
| CCTTCTCAGGCCAATCAAGG+CGG | + | chr8.4:6550713-6550732 | MS.gene88643:CDS | 55.0% | |
| CGGCGGTTTCATGCCTTCTC+AGG | + | chr8.4:6550700-6550719 | MS.gene88643:CDS | 60.0% | |
| ! | AGAAGGCATGAAACCGCCGC+CGG | - | chr8.4:6550699-6550718 | None:intergenic | 60.0% |
| ! | GACGGTAACGCCGCTTTCTC+CGG | + | chr8.4:6550677-6550696 | MS.gene88643:CDS | 60.0% |
| !! | CCGCCTTGATTGGCCTGAGA+AGG | - | chr8.4:6550716-6550735 | None:intergenic | 60.0% |
| GAAACCGCCGCCGGAGAAAG+CGG | - | chr8.4:6550690-6550709 | None:intergenic | 65.0% | |
| ! | GGTAACGCCGCTTTCTCCGG+CGG | + | chr8.4:6550680-6550699 | MS.gene88643:CDS | 65.0% |
| ! | AACGCCGCTTTCTCCGGCGG+CGG | + | chr8.4:6550683-6550702 | MS.gene88643:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 6550665 | 6551190 | 6550665 | ID=MS.gene88643 |
| chr8.4 | mRNA | 6550665 | 6551190 | 6550665 | ID=MS.gene88643.t1;Parent=MS.gene88643 |
| chr8.4 | exon | 6550665 | 6550760 | 6550665 | ID=MS.gene88643.t1.exon1;Parent=MS.gene88643.t1 |
| chr8.4 | CDS | 6550665 | 6550760 | 6550665 | ID=cds.MS.gene88643.t1;Parent=MS.gene88643.t1 |
| chr8.4 | exon | 6550904 | 6551014 | 6550904 | ID=MS.gene88643.t1.exon2;Parent=MS.gene88643.t1 |
| chr8.4 | CDS | 6550904 | 6551014 | 6550904 | ID=cds.MS.gene88643.t1;Parent=MS.gene88643.t1 |
| chr8.4 | exon | 6551089 | 6551190 | 6551089 | ID=MS.gene88643.t1.exon3;Parent=MS.gene88643.t1 |
| chr8.4 | CDS | 6551089 | 6551190 | 6551089 | ID=cds.MS.gene88643.t1;Parent=MS.gene88643.t1 |
| Gene Sequence |
| Protein sequence |
