Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88670.t1 | XP_003610146.1 | 99.2 | 132 | 1 | 0 | 1 | 132 | 1 | 132 | 1.60E-63 | 251.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88670.t1 | G7JD05 | 99.2 | 132 | 1 | 0 | 1 | 132 | 1 | 132 | 1.2e-63 | 251.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052053 | MS.gene88670 | 0.800217 | 1.60E-48 | -1.69E-46 |
| MS.gene055549 | MS.gene88670 | 0.816594 | 5.10E-52 | -1.69E-46 |
| MS.gene055550 | MS.gene88670 | 0.825525 | 4.47E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88670.t1 | MTR_4g128470 | 99.242 | 132 | 1 | 0 | 1 | 132 | 1 | 132 | 4.02e-94 | 267 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88670.t1 | AT5G57510 | 48.800 | 125 | 58 | 5 | 6 | 127 | 3 | 124 | 8.72e-27 | 97.1 |
Find 18 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAAGGAACAAAATAGTTC+TGG | 0.290844 | 8.1:+4487176 | MS.gene88670:CDS |
| CTCCATCTCCTTGTCTTCAA+AGG | 0.416512 | 8.1:+4487039 | MS.gene88670:CDS |
| CCTACGAACGGCGGTGCAAA+AGG | 0.478843 | 8.1:+4486860 | MS.gene88670:CDS |
| CTTATTGTCCTTTGAAGACA+AGG | 0.489660 | 8.1:-4487047 | None:intergenic |
| GTTCCATGTGTGATTGAGTA+TGG | 0.495036 | 8.1:-4486904 | None:intergenic |
| CTAAGTTTCAACGATCGTCC+TGG | 0.511331 | 8.1:+4486969 | MS.gene88670:CDS |
| GTCCTTTGAAGACAAGGAGA+TGG | 0.521798 | 8.1:-4487041 | None:intergenic |
| TTCAGATGAAACTGATTCAG+AGG | 0.544944 | 8.1:+4487007 | MS.gene88670:CDS |
| TCAGATGAAACTGATTCAGA+GGG | 0.570568 | 8.1:+4487008 | MS.gene88670:CDS |
| GAAGAACACATCATCAATCC+AGG | 0.580359 | 8.1:-4486987 | None:intergenic |
| TTTGCACCGCCGTTCGTAGG+CGG | 0.592132 | 8.1:-4486857 | None:intergenic |
| GGATGACATTGATAAGAGAT+CGG | 0.600101 | 8.1:+4487085 | MS.gene88670:CDS |
| TCAATGTCATCCTCATCTGA+AGG | 0.601031 | 8.1:-4487074 | None:intergenic |
| CCACCATACTCAATCACACA+TGG | 0.607769 | 8.1:+4486901 | MS.gene88670:CDS |
| ATTGCAGCTACGTTATTGCA+AGG | 0.608652 | 8.1:+4487160 | MS.gene88670:CDS |
| CCATGTGTGATTGAGTATGG+TGG | 0.643381 | 8.1:-4486901 | None:intergenic |
| TCTACTAAGCCGCCTACGAA+CGG | 0.672450 | 8.1:+4486848 | MS.gene88670:CDS |
| ACTAAGCCGCCTACGAACGG+CGG | 0.714763 | 8.1:+4486851 | MS.gene88670:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAAGTTTTCCTTCAGATG+AGG | + | chr8.1:4487064-4487083 | MS.gene88670:CDS | 30.0% |
| GGATGACATTGATAAGAGAT+CGG | + | chr8.1:4487085-4487104 | MS.gene88670:CDS | 35.0% | |
| TGCAAGGAACAAAATAGTTC+TGG | + | chr8.1:4487176-4487195 | MS.gene88670:CDS | 35.0% | |
| ! | CTTATTGTCCTTTGAAGACA+AGG | - | chr8.1:4487050-4487069 | None:intergenic | 35.0% |
| ! | TCAGATGAAACTGATTCAGA+GGG | + | chr8.1:4487008-4487027 | MS.gene88670:CDS | 35.0% |
| ! | TTCAGATGAAACTGATTCAG+AGG | + | chr8.1:4487007-4487026 | MS.gene88670:CDS | 35.0% |
| ATTGCAGCTACGTTATTGCA+AGG | + | chr8.1:4487160-4487179 | MS.gene88670:CDS | 40.0% | |
| GAAGAACACATCATCAATCC+AGG | - | chr8.1:4486990-4487009 | None:intergenic | 40.0% | |
| GTTCCATGTGTGATTGAGTA+TGG | - | chr8.1:4486907-4486926 | None:intergenic | 40.0% | |
| TCAATGTCATCCTCATCTGA+AGG | - | chr8.1:4487077-4487096 | None:intergenic | 40.0% | |
| AACGCTGCAAAAATGCTTCG+CGG | + | chr8.1:4486924-4486943 | MS.gene88670:CDS | 45.0% | |
| CCACCATACTCAATCACACA+TGG | + | chr8.1:4486901-4486920 | MS.gene88670:CDS | 45.0% | |
| CCATGTGTGATTGAGTATGG+TGG | - | chr8.1:4486904-4486923 | None:intergenic | 45.0% | |
| CTAAGTTTCAACGATCGTCC+TGG | + | chr8.1:4486969-4486988 | MS.gene88670:CDS | 45.0% | |
| CTCCATCTCCTTGTCTTCAA+AGG | + | chr8.1:4487039-4487058 | MS.gene88670:CDS | 45.0% | |
| ! | GTCCTTTGAAGACAAGGAGA+TGG | - | chr8.1:4487044-4487063 | None:intergenic | 45.0% |
| TCTACTAAGCCGCCTACGAA+CGG | + | chr8.1:4486848-4486867 | MS.gene88670:CDS | 50.0% | |
| ACTAAGCCGCCTACGAACGG+CGG | + | chr8.1:4486851-4486870 | MS.gene88670:CDS | 60.0% | |
| CCTACGAACGGCGGTGCAAA+AGG | + | chr8.1:4486860-4486879 | MS.gene88670:CDS | 60.0% | |
| TTTGCACCGCCGTTCGTAGG+CGG | - | chr8.1:4486860-4486879 | None:intergenic | 60.0% | |
| ! | CCTTTTGCACCGCCGTTCGT+AGG | - | chr8.1:4486863-4486882 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 4486816 | 4487214 | 4486816 | ID=MS.gene88670 |
| chr8.1 | mRNA | 4486816 | 4487214 | 4486816 | ID=MS.gene88670.t1;Parent=MS.gene88670 |
| chr8.1 | exon | 4486816 | 4487214 | 4486816 | ID=MS.gene88670.t1.exon1;Parent=MS.gene88670.t1 |
| chr8.1 | CDS | 4486816 | 4487214 | 4486816 | ID=cds.MS.gene88670.t1;Parent=MS.gene88670.t1 |
| Gene Sequence |
| Protein sequence |
