AlfalfaGEDB | MS.gene88767

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88767.t1	PNY10045.1	86.2	123	12	2	1	118	1	123	2.20E-43	184.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88767.t1	A0A2K3P419	86.2	123	12	2	1	118	1	123	1.6e-43	184.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene054801	MS.gene88767	0.819262	1.27E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88767.t1	MTR_5g069980	94.915	118	6	0	1	118	9	126	1.15e-63	189
MS.gene88767.t1	MTR_3g462960	54.206	107	46	2	10	113	2	108	1.95e-31	108
MS.gene88767.t1	MTR_3g462980	54.237	118	50	3	5	118	9	126	2.57e-29	103

Find 25 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAAATTCTAATAGAAAAT+CGG	0.330134	5.4:+54969078	MS.gene88767:CDS
AGAATCTTCGCGGGATGTTT+TGG	0.334299	5.4:-54969362	None:intergenic
CTCGGAGGAGATCATTAAAC+CGG	0.424159	5.4:+54969218	MS.gene88767:CDS
ATGCTTTACGCTTGGTCTCT+CGG	0.435166	5.4:+54969200	MS.gene88767:CDS
GGTCATCTGCTGATTCTCAT+AGG	0.439668	5.4:+54969275	MS.gene88767:CDS
CAAAATTCTAATAGAAAATC+GGG	0.444165	5.4:+54969079	MS.gene88767:CDS
CGGGTAGAAATCGACATGTT+AGG	0.447701	5.4:+54969098	MS.gene88767:CDS
AACCGGGACCCTTCTTCCTT+AGG	0.458094	5.4:+54969235	MS.gene88767:CDS
ATGTTAGGAGCTGTACATTC+AGG	0.460663	5.4:+54969113	MS.gene88767:CDS
CTCTCCCATTGAACTTGTCA+AGG	0.483017	5.4:+54969156	MS.gene88767:CDS
GCTGTACATTCAGGATTCCT+TGG	0.491004	5.4:+54969122	MS.gene88767:CDS
AAGATCCTTGACAAGTTCAA+TGG	0.492476	5.4:-54969161	None:intergenic
AGATCCTTGACAAGTTCAAT+GGG	0.508733	5.4:-54969160	None:intergenic
TTAGGAAGATCAGCATCAGC+TGG	0.514570	5.4:+54969253	MS.gene88767:CDS
GCTGATCTTCCTAAGGAAGA+AGG	0.517874	5.4:-54969244	None:intergenic
AGTGACAAATGCTTTACGCT+TGG	0.531836	5.4:+54969192	MS.gene88767:CDS
TTCCTAAGGAAGAAGGGTCC+CGG	0.543945	5.4:-54969237	None:intergenic
AGCTGATGCTGATCTTCCTA+AGG	0.546955	5.4:-54969251	None:intergenic
AGCTTCTATAGAATCTTCGC+GGG	0.555601	5.4:-54969371	None:intergenic
AAGCTTCTATAGAATCTTCG+CGG	0.574249	5.4:-54969372	None:intergenic
CTGATCTTCCTAAGGAAGAA+GGG	0.574518	5.4:-54969243	None:intergenic
TAGGAAGATCAGCATCAGCT+GGG	0.614174	5.4:+54969254	MS.gene88767:CDS
GGAGAGAATTCAGATTGCCA+AGG	0.633518	5.4:-54969139	None:intergenic
TCGGAGGAGATCATTAAACC+GGG	0.670094	5.4:+54969219	MS.gene88767:CDS
CTTTACGCTTGGTCTCTCGG+AGG	0.743015	5.4:+54969203	MS.gene88767:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACAAAATTCTAATAGAAAAT+CGG	+	chr5.4:54969078-54969097	MS.gene88767:CDS	15.0%
!!	CAAAATTCTAATAGAAAATC+GGG	+	chr5.4:54969079-54969098	MS.gene88767:CDS	20.0%
!	ACGAAAAAACAGGTTTTTCA+TGG	+	chr5.4:54969053-54969072	MS.gene88767:CDS	30.0%
	AAGATCCTTGACAAGTTCAA+TGG	-	chr5.4:54969164-54969183	None:intergenic	35.0%
	AAGCTTCTATAGAATCTTCG+CGG	-	chr5.4:54969375-54969394	None:intergenic	35.0%
	AGATCCTTGACAAGTTCAAT+GGG	-	chr5.4:54969163-54969182	None:intergenic	35.0%
	ATGAGTCACAACGAAAAAAC+AGG	+	chr5.4:54969043-54969062	MS.gene88767:CDS	35.0%
	TCGATCTAGAAAAAGAAGAG+TGG	-	chr5.4:54969333-54969352	None:intergenic	35.0%
	AGCTTCTATAGAATCTTCGC+GGG	-	chr5.4:54969374-54969393	None:intergenic	40.0%
	AGTGACAAATGCTTTACGCT+TGG	+	chr5.4:54969192-54969211	MS.gene88767:CDS	40.0%
	ATGTTAGGAGCTGTACATTC+AGG	+	chr5.4:54969113-54969132	MS.gene88767:CDS	40.0%
	CTGATCTTCCTAAGGAAGAA+GGG	-	chr5.4:54969246-54969265	None:intergenic	40.0%
	AGCTGATGCTGATCTTCCTA+AGG	-	chr5.4:54969254-54969273	None:intergenic	45.0%
	ATGCTTTACGCTTGGTCTCT+CGG	+	chr5.4:54969200-54969219	MS.gene88767:CDS	45.0%
	CGGGTAGAAATCGACATGTT+AGG	+	chr5.4:54969098-54969117	MS.gene88767:CDS	45.0%
	CTCGGAGGAGATCATTAAAC+CGG	+	chr5.4:54969218-54969237	MS.gene88767:CDS	45.0%
	CTCTCCCATTGAACTTGTCA+AGG	+	chr5.4:54969156-54969175	MS.gene88767:CDS	45.0%
	GCTGATCTTCCTAAGGAAGA+AGG	-	chr5.4:54969247-54969266	None:intergenic	45.0%
	GCTGTACATTCAGGATTCCT+TGG	+	chr5.4:54969122-54969141	MS.gene88767:CDS	45.0%
	GGAGAGAATTCAGATTGCCA+AGG	-	chr5.4:54969142-54969161	None:intergenic	45.0%
	TAGGAAGATCAGCATCAGCT+GGG	+	chr5.4:54969254-54969273	MS.gene88767:CDS	45.0%
	TCGGAGGAGATCATTAAACC+GGG	+	chr5.4:54969219-54969238	MS.gene88767:CDS	45.0%
	TTAGGAAGATCAGCATCAGC+TGG	+	chr5.4:54969253-54969272	MS.gene88767:CDS	45.0%
!	AGAATCTTCGCGGGATGTTT+TGG	-	chr5.4:54969365-54969384	None:intergenic	45.0%
!!	GGTCATCTGCTGATTCTCAT+AGG	+	chr5.4:54969275-54969294	MS.gene88767:CDS	45.0%
	TTCCTAAGGAAGAAGGGTCC+CGG	-	chr5.4:54969240-54969259	None:intergenic	50.0%
!!!	TTCGCGGGATGTTTTGGTAG+AGG	-	chr5.4:54969359-54969378	None:intergenic	50.0%
	AACCGGGACCCTTCTTCCTT+AGG	+	chr5.4:54969235-54969254	MS.gene88767:CDS	55.0%
	CTTTACGCTTGGTCTCTCGG+AGG	+	chr5.4:54969203-54969222	MS.gene88767:CDS	55.0%

Gene Sequence	>MS.gene88767 ATGAGTCACAACGAAAAAACAGGTTTTTCATGGAGACAAAATTCTAATAGAAAATCGGGTAGAAATCGACATGTTAGGAGCTGTACATTCAGGATTCCTTGGCAATCTGAATTCTCTCCCATTGAACTTGTCAAGGATCTTGCAGAAAGAGTGACAAATGCTTTACGCTTGGTCTCTCGGAGGAGATCATTAAACCGGGACCCTTCTTCCTTAGGAAGATCAGCATCAGCTGGGTCATCTGCTGATTCTCATAGGACAGCTGCTGTTGAAGATTGCATTGAATTCATCCACTCTTCTTTTTCTAGATCGAATTCCTCTACCAAAACATCCCGCGAAGATTCTATAGAAGCTTCTTGA
Protein sequence	>MS.gene88767.t1 MSHNEKTGFSWRQNSNRKSGRNRHVRSCTFRIPWQSEFSPIELVKDLAERVTNALRLVSRRRSLNRDPSSLGRSASAGSSADSHRTAAVEDCIEFIHSSFSRSNSSTKTSREDSIEAS

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	54969043	54969399	54969043	ID=MS.gene88767
chr5.4	mRNA	54969043	54969399	54969043	ID=MS.gene88767.t1;Parent=MS.gene88767
chr5.4	exon	54969043	54969399	54969043	ID=MS.gene88767.t1.exon1;Parent=MS.gene88767.t1
chr5.4	CDS	54969043	54969399	54969043	ID=cds.MS.gene88767.t1;Parent=MS.gene88767.t1

M. sativa cultivar XinJiangDaYe / MS.gene88767