Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88809.t1 | XP_013468822.1 | 100 | 77 | 0 | 0 | 1 | 77 | 57 | 133 | 2.00E-29 | 137.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88809.t1 | A0A072VLL2 | 100.0 | 77 | 0 | 0 | 1 | 77 | 57 | 133 | 1.4e-29 | 137.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057246 | MS.gene88809 | -0.808119 | 3.62E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88809.t1 | MTR_1g077210 | 100.000 | 77 | 0 | 0 | 1 | 77 | 57 | 133 | 5.41e-48 | 152 |
| MS.gene88809.t1 | MTR_1g009030 | 88.312 | 77 | 9 | 0 | 1 | 77 | 57 | 133 | 9.98e-43 | 137 |
| MS.gene88809.t1 | MTR_3g109280 | 88.312 | 77 | 9 | 0 | 1 | 77 | 57 | 133 | 1.26e-42 | 138 |
| MS.gene88809.t1 | MTR_0054s0080 | 88.312 | 77 | 9 | 0 | 1 | 77 | 57 | 133 | 2.55e-42 | 137 |
| MS.gene88809.t1 | MTR_6g066040 | 67.105 | 76 | 25 | 0 | 1 | 76 | 57 | 132 | 8.89e-32 | 110 |
| MS.gene88809.t1 | MTR_6g066090 | 64.407 | 59 | 21 | 0 | 18 | 76 | 1 | 59 | 1.28e-21 | 82.4 |
| MS.gene88809.t1 | MTR_5g068640 | 38.158 | 76 | 46 | 1 | 1 | 76 | 55 | 129 | 1.77e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88809.t1 | AT4G31330 | 84.416 | 77 | 12 | 0 | 1 | 77 | 57 | 133 | 1.23e-40 | 133 |
| MS.gene88809.t1 | AT5G10580 | 81.818 | 77 | 14 | 0 | 1 | 77 | 57 | 133 | 2.18e-40 | 131 |
| MS.gene88809.t1 | AT5G10580 | 81.818 | 77 | 14 | 0 | 1 | 77 | 57 | 133 | 9.85e-40 | 131 |
| MS.gene88809.t1 | AT5G10580 | 81.818 | 77 | 14 | 0 | 1 | 77 | 57 | 133 | 9.85e-40 | 131 |
| MS.gene88809.t1 | AT5G10580 | 81.818 | 77 | 14 | 0 | 1 | 77 | 57 | 133 | 1.18e-39 | 131 |
| MS.gene88809.t1 | AT5G24790 | 68.571 | 70 | 22 | 0 | 1 | 70 | 57 | 126 | 3.38e-31 | 109 |
Find 14 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCTCAATGATTCCATTTA+TGG | 0.062471 | 1.1:-58100516 | MS.gene88809:CDS |
| TGGAGCTCATAGTGAATTTA+TGG | 0.212693 | 1.1:-58100496 | MS.gene88809:CDS |
| AACCCTACGTAATCTAATAA+TGG | 0.254699 | 1.1:-58100622 | MS.gene88809:CDS |
| TTCACTATGAGCTCCATAAA+TGG | 0.279592 | 1.1:+58100503 | None:intergenic |
| AATAATGGGATCAACACTTA+TGG | 0.371912 | 1.1:-58100607 | MS.gene88809:CDS |
| CATTCTACTCTCAGCTGGTT+TGG | 0.383869 | 1.1:-58100574 | MS.gene88809:CDS |
| ACCCTACGTAATCTAATAAT+GGG | 0.403558 | 1.1:-58100621 | MS.gene88809:CDS |
| ATGAGAATAAGAATATAGTT+AGG | 0.446792 | 1.1:+58100451 | None:intergenic |
| TCCCATTATTAGATTACGTA+GGG | 0.517944 | 1.1:+58100620 | None:intergenic |
| ACATCCATTCTACTCTCAGC+TGG | 0.523277 | 1.1:-58100579 | MS.gene88809:CDS |
| CAAACCAGCTGAGAGTAGAA+TGG | 0.570136 | 1.1:+58100575 | None:intergenic |
| CCATAAATGGAATCATTGAG+AGG | 0.651845 | 1.1:+58100516 | None:intergenic |
| TACGTAGGGTTTGAACAGCT+AGG | 0.661798 | 1.1:+58100634 | None:intergenic |
| ATCCCATTATTAGATTACGT+AGG | 0.669460 | 1.1:+58100619 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGAATAAGAATATAGTT+AGG | + | chr1.1:58100651-58100670 | None:intergenic | 20.0% |
| AACCCTACGTAATCTAATAA+TGG | - | chr1.1:58100477-58100496 | MS.gene88809:CDS | 30.0% | |
| ACCCTACGTAATCTAATAAT+GGG | - | chr1.1:58100478-58100497 | MS.gene88809:CDS | 30.0% | |
| ATCCCATTATTAGATTACGT+AGG | + | chr1.1:58100483-58100502 | None:intergenic | 30.0% | |
| TCCCATTATTAGATTACGTA+GGG | + | chr1.1:58100482-58100501 | None:intergenic | 30.0% | |
| ! | AATAATGGGATCAACACTTA+TGG | - | chr1.1:58100492-58100511 | MS.gene88809:CDS | 30.0% |
| !! | TAGGAGTGTGACATATTTTA+GGG | + | chr1.1:58100632-58100651 | None:intergenic | 30.0% |
| !! | TTAGGAGTGTGACATATTTT+AGG | + | chr1.1:58100633-58100652 | None:intergenic | 30.0% |
| CCATAAATGGAATCATTGAG+AGG | + | chr1.1:58100586-58100605 | None:intergenic | 35.0% | |
| CCTCTCAATGATTCCATTTA+TGG | - | chr1.1:58100583-58100602 | MS.gene88809:CDS | 35.0% | |
| TGGAGCTCATAGTGAATTTA+TGG | - | chr1.1:58100603-58100622 | MS.gene88809:CDS | 35.0% | |
| TTCACTATGAGCTCCATAAA+TGG | + | chr1.1:58100599-58100618 | None:intergenic | 35.0% | |
| ACATCCATTCTACTCTCAGC+TGG | - | chr1.1:58100520-58100539 | MS.gene88809:CDS | 45.0% | |
| CAAACCAGCTGAGAGTAGAA+TGG | + | chr1.1:58100527-58100546 | None:intergenic | 45.0% | |
| CATTCTACTCTCAGCTGGTT+TGG | - | chr1.1:58100525-58100544 | MS.gene88809:CDS | 45.0% | |
| TACGTAGGGTTTGAACAGCT+AGG | + | chr1.1:58100468-58100487 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 58100445 | 58100676 | 58100445 | ID=MS.gene88809 |
| chr1.1 | mRNA | 58100445 | 58100676 | 58100445 | ID=MS.gene88809.t1;Parent=MS.gene88809 |
| chr1.1 | exon | 58100445 | 58100676 | 58100445 | ID=MS.gene88809.t1.exon1;Parent=MS.gene88809.t1 |
| chr1.1 | CDS | 58100445 | 58100676 | 58100445 | ID=cds.MS.gene88809.t1;Parent=MS.gene88809.t1 |
| Gene Sequence |
| Protein sequence |
