Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88856.t1 | XP_024641661.1 | 95.3 | 445 | 21 | 0 | 1 | 445 | 1 | 445 | 4.10E-244 | 853.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88856.t1 | Q9JI38 | 40.9 | 369 | 195 | 7 | 90 | 437 | 61 | 427 | 1.7e-65 | 251.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88856.t1 | A0A396K0J5 | 95.3 | 445 | 21 | 0 | 1 | 445 | 1 | 445 | 3.0e-244 | 853.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050156 | MS.gene88856 | 0.806838 | 6.77E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88856.t1 | MTR_1g097390 | 95.685 | 394 | 17 | 0 | 1 | 394 | 1 | 394 | 0.0 | 794 |
| MS.gene88856.t1 | MTR_4g123970 | 28.685 | 251 | 130 | 8 | 97 | 328 | 46 | 266 | 2.41e-18 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88856.t1 | AT1G34150 | 56.308 | 428 | 164 | 4 | 19 | 441 | 25 | 434 | 2.23e-170 | 486 |
| MS.gene88856.t1 | AT3G06950 | 29.180 | 305 | 165 | 9 | 97 | 379 | 45 | 320 | 7.63e-24 | 102 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGCGACATTCGAATTCT+TGG | 0.178244 | 1.1:-70099793 | MS.gene88856:CDS |
| CTTTAGAAACTTCTGTAAAA+TGG | 0.194487 | 1.1:-70098275 | MS.gene88856:CDS |
| AAGTTATGTACTTTGGTAAA+AGG | 0.235850 | 1.1:-70102579 | MS.gene88856:intron |
| CCTTCTAAATACAGGTTCTA+TGG | 0.259215 | 1.1:-70101761 | MS.gene88856:intron |
| GGGAGTATAGATACTTCTTT+TGG | 0.270110 | 1.1:-70098497 | MS.gene88856:CDS |
| GATATAGGAGCCTTTCTTCT+TGG | 0.298392 | 1.1:+70096031 | None:intergenic |
| GGAGTAAATAATGGAAGTTC+TGG | 0.316799 | 1.1:-70100866 | MS.gene88856:CDS |
| TAATACAGGTTCAGCTGTTC+AGG | 0.318276 | 1.1:-70098522 | MS.gene88856:intron |
| TTCTTCTTGGATCCATGAAT+TGG | 0.333541 | 1.1:+70096044 | None:intergenic |
| TCACGTGCATGAGCTGAGTT+TGG | 0.368699 | 1.1:+70095772 | None:intergenic |
| TCTTGAGACAACAAGGCTAT+TGG | 0.372999 | 1.1:-70101471 | MS.gene88856:CDS |
| GAATCCAAGCCACCAATTCA+TGG | 0.391582 | 1.1:-70096056 | MS.gene88856:CDS |
| TATGGCTTCAGAGGCTCCTT+TGG | 0.394080 | 1.1:-70096470 | MS.gene88856:CDS |
| AAAATGTCTGGAGTAAATAA+TGG | 0.398496 | 1.1:-70100875 | MS.gene88856:CDS |
| GGTGGCTTGGATTCTTGAAT+TGG | 0.401915 | 1.1:+70096065 | None:intergenic |
| TTCTTGGATCCATGAATTGG+TGG | 0.403111 | 1.1:+70096047 | None:intergenic |
| CTCAAGCTTATATGTTCATC+AGG | 0.417049 | 1.1:-70096421 | MS.gene88856:intron |
| TCGTGACATGAGAGGGATAT+AGG | 0.419584 | 1.1:+70096016 | None:intergenic |
| CAGCAACCATGCAGCGAACC+TGG | 0.427482 | 1.1:+70097280 | None:intergenic |
| AGATCAAAGCTCAAAATGTC+TGG | 0.430701 | 1.1:-70100887 | MS.gene88856:CDS |
| GAAAGATTGCAGTTTGAAGC+TGG | 0.435801 | 1.1:+70096233 | None:intergenic |
| AACCTTGCATGGAAATCAAC+AGG | 0.440517 | 1.1:+70099757 | None:intergenic |
| GTTGCTGTTCTCTTCTCGAT+TGG | 0.441960 | 1.1:-70097264 | MS.gene88856:CDS |
| GCTTTAAAAGTTATGTACTT+TGG | 0.453248 | 1.1:-70102586 | MS.gene88856:CDS |
| ACATCACCACCTTGCAAGCC+AGG | 0.457082 | 1.1:-70103645 | MS.gene88856:CDS |
| ACATGCTACTGGACACCAAC+AGG | 0.457973 | 1.1:-70096516 | MS.gene88856:CDS |
| GGTATGAGGATAATCAGCTT+TGG | 0.458695 | 1.1:-70097338 | MS.gene88856:CDS |
| GTATGAGGATAATCAGCTTT+GGG | 0.460075 | 1.1:-70097337 | MS.gene88856:CDS |
| CAGAAAACCTCAGTACGTTA+TGG | 0.462009 | 1.1:-70096488 | MS.gene88856:CDS |
| GGCTTGGATTCTTGAATTGG+TGG | 0.463834 | 1.1:+70096068 | None:intergenic |
| CTAGGTGATTGACATGCTAC+TGG | 0.467264 | 1.1:-70096527 | MS.gene88856:intron |
| GCATGGAAATCAACAGGAAC+AGG | 0.470296 | 1.1:+70099763 | None:intergenic |
| GGATCCATGAATTGGTGGCT+TGG | 0.479927 | 1.1:+70096052 | None:intergenic |
| GAAAGAAAATTTGAATATCT+CGG | 0.480631 | 1.1:-70098475 | MS.gene88856:intron |
| CGTCTCTTCTGATTTCCCGT+TGG | 0.490374 | 1.1:+70103095 | None:intergenic |
| GTTCTCTTCTCGATTGGCAA+AGG | 0.496553 | 1.1:-70097258 | MS.gene88856:CDS |
| TTCCTGTTGATTTCCATGCA+AGG | 0.496812 | 1.1:-70099759 | MS.gene88856:intron |
| ACAGGTTCAGCTGTTCAGGC+AGG | 0.498462 | 1.1:-70098518 | MS.gene88856:intron |
| AATTGTGTGCCTCTATTACA+TGG | 0.503981 | 1.1:-70096196 | MS.gene88856:intron |
| AATGCTGGAACAAAACTAGT+AGG | 0.505399 | 1.1:-70098307 | MS.gene88856:CDS |
| AAAAGCGCTGCATGCACACT+TGG | 0.509337 | 1.1:-70096275 | MS.gene88856:CDS |
| TACCTTAACCCTGGCTTGCA+AGG | 0.510643 | 1.1:+70103636 | None:intergenic |
| GAATGTATGCAAACCTTGCA+TGG | 0.513078 | 1.1:+70099746 | None:intergenic |
| CACCTTGCAAGCCAGGGTTA+AGG | 0.513309 | 1.1:-70103638 | MS.gene88856:intron |
| GCTATTGGTTGGTGATAAGA+AGG | 0.520717 | 1.1:-70101456 | MS.gene88856:CDS |
| CAGAACCGAAAATATCCAAC+GGG | 0.522953 | 1.1:-70103110 | MS.gene88856:CDS |
| CATCAAACTACGAGGAGCAT+GGG | 0.523045 | 1.1:-70103691 | MS.gene88856:CDS |
| CCAAGAATTCGAATGTCGCT+TGG | 0.523997 | 1.1:+70099793 | None:intergenic |
| GCGACATTCGAATTCTTGGC+TGG | 0.528981 | 1.1:-70099789 | MS.gene88856:CDS |
| ATGGCACCAGGTTCGCTGCA+TGG | 0.530252 | 1.1:-70097286 | MS.gene88856:CDS |
| TTTGCAGCACCATGTAATAG+AGG | 0.535715 | 1.1:+70096187 | None:intergenic |
| TTCGTTTCTGAAGAGCAACT+TGG | 0.540628 | 1.1:-70100839 | MS.gene88856:intron |
| ATTCATGGATCCAAGAAGAA+AGG | 0.546667 | 1.1:-70096041 | MS.gene88856:CDS |
| AAGGGGAAATTGATTATGTG+AGG | 0.553779 | 1.1:-70099834 | MS.gene88856:CDS |
| CATTAGGCTATGGAGAATGC+TGG | 0.555814 | 1.1:-70098322 | MS.gene88856:intron |
| GATGTGGTAGAACAGACAAA+GGG | 0.566299 | 1.1:-70101418 | MS.gene88856:CDS |
| TCAAAGCTCTTGAGACAACA+AGG | 0.569509 | 1.1:-70101478 | MS.gene88856:CDS |
| GCATCAAACTACGAGGAGCA+TGG | 0.569888 | 1.1:-70103692 | MS.gene88856:CDS |
| AGGGGAAATTGATTATGTGA+GGG | 0.574580 | 1.1:-70099833 | MS.gene88856:CDS |
| TGCCAGAACTCACGCTCAGT+TGG | 0.576326 | 1.1:+70095993 | None:intergenic |
| CCAACTTACTTGACCAACAG+AGG | 0.576982 | 1.1:+70101391 | None:intergenic |
| GAGACAACAAGGCTATTGGT+TGG | 0.585415 | 1.1:-70101467 | MS.gene88856:CDS |
| CAGTTGGTCGTGACATGAGA+GGG | 0.591980 | 1.1:+70096009 | None:intergenic |
| CATCACCACCTTGCAAGCCA+GGG | 0.592432 | 1.1:-70103644 | MS.gene88856:CDS |
| CATTGTGATACGCCGCATGT+AGG | 0.593237 | 1.1:+70098234 | None:intergenic |
| GACAAAGGGGTATCCTCTGT+TGG | 0.597429 | 1.1:-70101404 | MS.gene88856:CDS |
| CGATGTGGTAGAACAGACAA+AGG | 0.597908 | 1.1:-70101419 | MS.gene88856:CDS |
| CAGGTTCAGCTGTTCAGGCA+GGG | 0.602249 | 1.1:-70098517 | MS.gene88856:CDS |
| ACAAATGGAACCATCTGTTG+AGG | 0.608651 | 1.1:-70101723 | MS.gene88856:intron |
| TCAGTACGTTATGGCTTCAG+AGG | 0.610268 | 1.1:-70096479 | MS.gene88856:CDS |
| TCAGTTGGTCGTGACATGAG+AGG | 0.623226 | 1.1:+70096008 | None:intergenic |
| AGATTTATGCCACAGATGTG+AGG | 0.626380 | 1.1:-70098207 | MS.gene88856:intron |
| TCACAGGACTGAAGAACCAA+AGG | 0.629124 | 1.1:+70096454 | None:intergenic |
| CAATCTTAACCTCACATCTG+TGG | 0.641581 | 1.1:+70098198 | None:intergenic |
| CTTAACCCTGGCTTGCAAGG+TGG | 0.645850 | 1.1:+70103639 | None:intergenic |
| ATGCTCCTCGTAGTTTGATG+CGG | 0.648498 | 1.1:+70103694 | None:intergenic |
| CGCTCATTGTCACTGCTCCG+AGG | 0.656391 | 1.1:-70103388 | MS.gene88856:intron |
| AAAGTACAGTACCTTAACCC+TGG | 0.658561 | 1.1:+70103627 | None:intergenic |
| CTTTGGGTGATTAAAATCAG+AGG | 0.660121 | 1.1:-70097321 | MS.gene88856:CDS |
| CAAATGTGCACACCTACATG+CGG | 0.661945 | 1.1:-70098246 | MS.gene88856:CDS |
| ACAGAACCGAAAATATCCAA+CGG | 0.664654 | 1.1:-70103111 | MS.gene88856:CDS |
| ATCAAACTACGAGGAGCATG+GGG | 0.668088 | 1.1:-70103690 | MS.gene88856:CDS |
| TCTGAAGCCATAACGTACTG+AGG | 0.670877 | 1.1:+70096481 | None:intergenic |
| GTGATACAGGCAATAACACA+AGG | 0.671483 | 1.1:-70102639 | MS.gene88856:intron |
| GAGTCACAATATTCGCGATG+TGG | 0.676266 | 1.1:-70101434 | MS.gene88856:CDS |
| ATGTGGTAGAACAGACAAAG+GGG | 0.734311 | 1.1:-70101417 | MS.gene88856:CDS |
| GAATGCCGCATCAAACTACG+AGG | 0.791038 | 1.1:-70103699 | MS.gene88856:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70095772 | 70103719 | 70095772 | ID=MS.gene88856 |
| chr1.1 | mRNA | 70095772 | 70103719 | 70095772 | ID=MS.gene88856.t1;Parent=MS.gene88856 |
| chr1.1 | exon | 70103639 | 70103719 | 70103639 | ID=MS.gene88856.t1.exon1;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70103639 | 70103719 | 70103639 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70103389 | 70103448 | 70103389 | ID=MS.gene88856.t1.exon2;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70103389 | 70103448 | 70103389 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70103079 | 70103178 | 70103079 | ID=MS.gene88856.t1.exon3;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70103079 | 70103178 | 70103079 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70102580 | 70102652 | 70102580 | ID=MS.gene88856.t1.exon4;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70102580 | 70102652 | 70102580 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70101724 | 70101769 | 70101724 | ID=MS.gene88856.t1.exon5;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70101724 | 70101769 | 70101724 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70101400 | 70101510 | 70101400 | ID=MS.gene88856.t1.exon6;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70101400 | 70101510 | 70101400 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70100840 | 70100927 | 70100840 | ID=MS.gene88856.t1.exon7;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70100840 | 70100927 | 70100840 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70099760 | 70099856 | 70099760 | ID=MS.gene88856.t1.exon8;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70099760 | 70099856 | 70099760 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70098476 | 70098536 | 70098476 | ID=MS.gene88856.t1.exon9;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70098476 | 70098536 | 70098476 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70098208 | 70098338 | 70098208 | ID=MS.gene88856.t1.exon10;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70098208 | 70098338 | 70098208 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70097242 | 70097359 | 70097242 | ID=MS.gene88856.t1.exon11;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70097242 | 70097359 | 70097242 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70096422 | 70096545 | 70096422 | ID=MS.gene88856.t1.exon12;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70096422 | 70096545 | 70096422 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70096197 | 70096304 | 70096197 | ID=MS.gene88856.t1.exon13;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70096197 | 70096304 | 70096197 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70096006 | 70096101 | 70096006 | ID=MS.gene88856.t1.exon14;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70096006 | 70096101 | 70096006 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| chr1.1 | exon | 70095772 | 70095815 | 70095772 | ID=MS.gene88856.t1.exon15;Parent=MS.gene88856.t1 |
| chr1.1 | CDS | 70095772 | 70095815 | 70095772 | ID=cds.MS.gene88856.t1;Parent=MS.gene88856.t1 |
| Gene Sequence |
| Protein sequence |
