Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88891.t1 | XP_003592048.2 | 98.6 | 144 | 2 | 0 | 1 | 144 | 1034 | 1177 | 1.50E-75 | 292 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88891.t1 | Q9SQI2 | 68.2 | 148 | 43 | 2 | 1 | 144 | 1024 | 1171 | 3.3e-50 | 199.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88891.t1 | G7IBU3 | 98.6 | 144 | 2 | 0 | 1 | 144 | 1034 | 1177 | 1.1e-75 | 292.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057249 | MS.gene88891 | 0.82369 | 1.21E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88891.t1 | MTR_1g098160 | 98.611 | 144 | 2 | 0 | 1 | 144 | 1034 | 1177 | 3.85e-94 | 295 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88891.t1 | AT1G22770 | 68.243 | 148 | 43 | 2 | 1 | 144 | 1024 | 1171 | 1.13e-60 | 201 |
Find 32 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGTTTATTCGCAGTGGTTT+AGG | 0.252575 | 1.1:-70607748 | None:intergenic |
| ATTCAGCCAGTGCTTGAGTT+TGG | 0.346143 | 1.1:+70607288 | MS.gene88891:CDS |
| ACGACGTCCAACTTGAAGTA+TGG | 0.355036 | 1.1:-70607790 | None:intergenic |
| GCAGTGTCAAGAAACTTTAT+AGG | 0.363840 | 1.1:-70607880 | None:intergenic |
| AGCTTGTACTTTACCACAGC+TGG | 0.413705 | 1.1:+70606716 | MS.gene88891:CDS |
| CAACTTGAAGTATGGATAAG+AGG | 0.421383 | 1.1:-70607782 | None:intergenic |
| GAAGTTTGTTTACCTTCAAA+AGG | 0.429452 | 1.1:-70607347 | None:intergenic |
| CCTAAACCACTGCGAATAAA+CGG | 0.439715 | 1.1:+70607748 | MS.gene88891:CDS |
| AGGACTACACCTTATTGAAC+CGG | 0.444643 | 1.1:-70607728 | None:intergenic |
| TCAAGTTGGACGTCGTTGAT+TGG | 0.452510 | 1.1:+70607797 | MS.gene88891:CDS |
| GTGCTTGAGTTTGGAGAACC+AGG | 0.473833 | 1.1:+70607297 | MS.gene88891:CDS |
| CTCTTATCCATACTTCAAGT+TGG | 0.475092 | 1.1:+70607783 | MS.gene88891:CDS |
| ACAGATGGGATGCTTGTTGA+TGG | 0.476223 | 1.1:+70606690 | None:intergenic |
| CCATCTGCTACAGCCAATCC+TGG | 0.478975 | 1.1:-70607315 | None:intergenic |
| GCTCACAGCAGAATTTCTGC+TGG | 0.488260 | 1.1:+70607853 | MS.gene88891:CDS |
| TTACCTTCAAAAGGTTTGAA+AGG | 0.507336 | 1.1:-70607338 | None:intergenic |
| GGTTTCACTTCCATTTCATA+CGG | 0.517859 | 1.1:-70607929 | None:intergenic |
| GTCGTCTAGCAGCTACCATT+AGG | 0.520793 | 1.1:+70607638 | MS.gene88891:CDS |
| TGGATGACAAAGGCACCTAA+TGG | 0.536734 | 1.1:-70607653 | None:intergenic |
| TGAGTTTGGAGAACCAGGAT+TGG | 0.545639 | 1.1:+70607302 | MS.gene88891:CDS |
| GGTTCTCCAAACTCAAGCAC+TGG | 0.546771 | 1.1:-70607294 | None:intergenic |
| AACTTGAAGTATGGATAAGA+GGG | 0.553585 | 1.1:-70607781 | None:intergenic |
| AGAGCACGGACATGTGCACT+TGG | 0.561271 | 1.1:-70607673 | None:intergenic |
| CCAGGATTGGCTGTAGCAGA+TGG | 0.565829 | 1.1:+70607315 | MS.gene88891:CDS |
| AGTATGGATAAGAGGGATTG+TGG | 0.581698 | 1.1:-70607774 | None:intergenic |
| GACACTGCTGCTAAAGAACT+AGG | 0.582788 | 1.1:+70607895 | MS.gene88891:CDS |
| TGGCTGCCGTTTATTCGCAG+TGG | 0.589244 | 1.1:-70607754 | None:intergenic |
| AACCGGTATGCAAAATGTCA+CGG | 0.603366 | 1.1:-70607711 | None:intergenic |
| GAACTGAGACACTCAGAGCA+CGG | 0.621434 | 1.1:-70607687 | None:intergenic |
| TTGTACTTTACCACAGCTGG+AGG | 0.642883 | 1.1:+70606719 | MS.gene88891:CDS |
| TAACATTCTACCTCCAGCTG+TGG | 0.669437 | 1.1:-70606729 | None:intergenic |
| CATGTGCACTTGGATGACAA+AGG | 0.690430 | 1.1:-70607663 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAATTAAAGAACTAAA+AGG | + | chr1.1:70606921-70606940 | MS.gene88891:intron | 10.0% |
| !! | AAAAAAATTGATGTAGTTAA+TGG | - | chr1.1:70606783-70606802 | None:intergenic | 15.0% |
| !!! | AAATTAATAGTTTTCAACAA+GGG | - | chr1.1:70607372-70607391 | None:intergenic | 15.0% |
| !!! | TAAATTAATAGTTTTCAACA+AGG | - | chr1.1:70607373-70607392 | None:intergenic | 15.0% |
| !!! | CATCTATTTGGATAATATTT+TGG | + | chr1.1:70607499-70607518 | MS.gene88891:intron | 20.0% |
| !!! | TTTCTAAGATCTGAATTTTT+AGG | - | chr1.1:70607089-70607108 | None:intergenic | 20.0% |
| ! | AATAGATGAGATCAGTTAAA+TGG | - | chr1.1:70607487-70607506 | None:intergenic | 25.0% |
| ! | TTAAAGAACTAAAAGGTCAA+AGG | + | chr1.1:70606928-70606947 | MS.gene88891:intron | 25.0% |
| ! | TTTAACTGATCTCATCTATT+TGG | + | chr1.1:70607487-70607506 | MS.gene88891:intron | 25.0% |
| !! | TCATTTTGTAGCTTTTCAAT+TGG | + | chr1.1:70607059-70607078 | MS.gene88891:intron | 25.0% |
| !! | TTTGATTAATGACTAGCTAT+CGG | + | chr1.1:70607164-70607183 | MS.gene88891:intron | 25.0% |
| !!! | TATTTCTATGTTGATGATGA+TGG | + | chr1.1:70607237-70607256 | MS.gene88891:intron | 25.0% |
| AACTGTTCTATCATGAAACA+TGG | + | chr1.1:70607430-70607449 | MS.gene88891:intron | 30.0% | |
| AACTTGAAGTATGGATAAGA+GGG | - | chr1.1:70607784-70607803 | None:intergenic | 30.0% | |
| TATGAGTATTAGCAAAGCTT+CGG | - | chr1.1:70607219-70607238 | None:intergenic | 30.0% | |
| TTACCTTCAAAAGGTTTGAA+AGG | - | chr1.1:70607341-70607360 | None:intergenic | 30.0% | |
| ! | CAAAGCACTTAAAAGTTGTT+TGG | - | chr1.1:70607558-70607577 | None:intergenic | 30.0% |
| ! | GAAGTTTGTTTACCTTCAAA+AGG | - | chr1.1:70607350-70607369 | None:intergenic | 30.0% |
| ! | TTTCTGATAAACGTTTTCCA+TGG | + | chr1.1:70606832-70606851 | MS.gene88891:intron | 30.0% |
| ACATAGAAAAATGCTTGGCA+TGG | + | chr1.1:70607827-70607846 | MS.gene88891:CDS | 35.0% | |
| AGCTGACATAGAAAAATGCT+TGG | + | chr1.1:70607822-70607841 | MS.gene88891:CDS | 35.0% | |
| CAACTTGAAGTATGGATAAG+AGG | - | chr1.1:70607785-70607804 | None:intergenic | 35.0% | |
| CATAGAAAAATGCTTGGCAT+GGG | + | chr1.1:70607828-70607847 | MS.gene88891:CDS | 35.0% | |
| CTCTTATCCATACTTCAAGT+TGG | + | chr1.1:70607783-70607802 | MS.gene88891:CDS | 35.0% | |
| GCAGTGTCAAGAAACTTTAT+AGG | - | chr1.1:70607883-70607902 | None:intergenic | 35.0% | |
| TCGGTATAGAATGCAGTATA+TGG | - | chr1.1:70607200-70607219 | None:intergenic | 35.0% | |
| TTAAATGTGCAATTACGAGC+TGG | - | chr1.1:70607525-70607544 | None:intergenic | 35.0% | |
| !! | TTCTTGCAACCAAGTATTGA+GGG | + | chr1.1:70607006-70607025 | MS.gene88891:intron | 35.0% |
| !!! | TTTTGCATACCGGTTCAATA+AGG | + | chr1.1:70607719-70607738 | MS.gene88891:CDS | 35.0% |
| AACCGGTATGCAAAATGTCA+CGG | - | chr1.1:70607714-70607733 | None:intergenic | 40.0% | |
| AAGGAGTGACAATGACTTGA+TGG | - | chr1.1:70606882-70606901 | None:intergenic | 40.0% | |
| AGGACTACACCTTATTGAAC+CGG | - | chr1.1:70607731-70607750 | None:intergenic | 40.0% | |
| AGTATGGATAAGAGGGATTG+TGG | - | chr1.1:70607777-70607796 | None:intergenic | 40.0% | |
| ATCAAGTCATTGTCACTCCT+TGG | + | chr1.1:70606881-70606900 | MS.gene88891:intron | 40.0% | |
| CATGAAACATGGCTCATGAT+AGG | + | chr1.1:70607441-70607460 | MS.gene88891:intron | 40.0% | |
| CCTAAACCACTGCGAATAAA+CGG | + | chr1.1:70607748-70607767 | MS.gene88891:CDS | 40.0% | |
| TTGAGTACACCCTCAATACT+TGG | - | chr1.1:70607018-70607037 | None:intergenic | 40.0% | |
| ! | GTTCTTGCAACCAAGTATTG+AGG | + | chr1.1:70607005-70607024 | MS.gene88891:intron | 40.0% |
| ! | TGGCCTTTCAAACCTTTTGA+AGG | + | chr1.1:70607335-70607354 | MS.gene88891:CDS | 40.0% |
| AAAGGCAATTTCGTGCTGTG+TGG | + | chr1.1:70606946-70606965 | MS.gene88891:intron | 45.0% | |
| AAGTCATTGTCACTCCTTGG+TGG | + | chr1.1:70606884-70606903 | MS.gene88891:intron | 45.0% | |
| ACGACGTCCAACTTGAAGTA+TGG | - | chr1.1:70607793-70607812 | None:intergenic | 45.0% | |
| AGCTTGTACTTTACCACAGC+TGG | + | chr1.1:70606716-70606735 | MS.gene88891:CDS | 45.0% | |
| ATTCAGCCAGTGCTTGAGTT+TGG | + | chr1.1:70607288-70607307 | MS.gene88891:CDS | 45.0% | |
| CATGTGCACTTGGATGACAA+AGG | - | chr1.1:70607666-70607685 | None:intergenic | 45.0% | |
| CCGTTTATTCGCAGTGGTTT+AGG | - | chr1.1:70607751-70607770 | None:intergenic | 45.0% | |
| GACACTGCTGCTAAAGAACT+AGG | + | chr1.1:70607895-70607914 | MS.gene88891:CDS | 45.0% | |
| TAACATTCTACCTCCAGCTG+TGG | - | chr1.1:70606732-70606751 | None:intergenic | 45.0% | |
| TGAGTTTGGAGAACCAGGAT+TGG | + | chr1.1:70607302-70607321 | MS.gene88891:CDS | 45.0% | |
| TTGTACTTTACCACAGCTGG+AGG | + | chr1.1:70606719-70606738 | MS.gene88891:CDS | 45.0% | |
| ! | AATGACTTGATGGCAGGGAT+GGG | - | chr1.1:70606872-70606891 | None:intergenic | 45.0% |
| ! | AGTGACAATGACTTGATGGC+AGG | - | chr1.1:70606878-70606897 | None:intergenic | 45.0% |
| ! | CTCCGTGACATTTTGCATAC+CGG | + | chr1.1:70607709-70607728 | MS.gene88891:CDS | 45.0% |
| ! | GTGACAATGACTTGATGGCA+GGG | - | chr1.1:70606877-70606896 | None:intergenic | 45.0% |
| !! | TCAAGTTGGACGTCGTTGAT+TGG | + | chr1.1:70607797-70607816 | MS.gene88891:CDS | 45.0% |
| !! | TGATGATGATGGCCAGCTAT+TGG | + | chr1.1:70607248-70607267 | MS.gene88891:intron | 45.0% |
| !! | TGGATGACAAAGGCACCTAA+TGG | - | chr1.1:70607656-70607675 | None:intergenic | 45.0% |
| GAACTGAGACACTCAGAGCA+CGG | - | chr1.1:70607690-70607709 | None:intergenic | 50.0% | |
| GCTCACAGCAGAATTTCTGC+TGG | + | chr1.1:70607853-70607872 | MS.gene88891:CDS | 50.0% | |
| GGGTGCGTAAGAAAGAACCA+TGG | - | chr1.1:70606852-70606871 | None:intergenic | 50.0% | |
| GGTTCTCCAAACTCAAGCAC+TGG | - | chr1.1:70607297-70607316 | None:intergenic | 50.0% | |
| GTCGTCTAGCAGCTACCATT+AGG | + | chr1.1:70607638-70607657 | MS.gene88891:CDS | 50.0% | |
| GTGCTTGAGTTTGGAGAACC+AGG | + | chr1.1:70607297-70607316 | MS.gene88891:CDS | 50.0% | |
| TTAAGACCCTGTGACCACCA+AGG | - | chr1.1:70606901-70606920 | None:intergenic | 50.0% | |
| ! | CAATGACTTGATGGCAGGGA+TGG | - | chr1.1:70606873-70606892 | None:intergenic | 50.0% |
| ! | TAGCTGTCGCTTCCAATAGC+TGG | - | chr1.1:70607263-70607282 | None:intergenic | 50.0% |
| AGAGCACGGACATGTGCACT+TGG | - | chr1.1:70607676-70607695 | None:intergenic | 55.0% | |
| CCATCTGCTACAGCCAATCC+TGG | - | chr1.1:70607318-70607337 | None:intergenic | 55.0% | |
| CTGCTAGACGACACTGCAAG+TGG | - | chr1.1:70607630-70607649 | None:intergenic | 55.0% | |
| GCAATTTCGTGCTGTGTGGC+AGG | + | chr1.1:70606950-70606969 | MS.gene88891:intron | 55.0% | |
| ! | GTCACTCCTTGGTGGTCACA+GGG | + | chr1.1:70606892-70606911 | MS.gene88891:intron | 55.0% |
| ! | TGGCTGCCGTTTATTCGCAG+TGG | - | chr1.1:70607757-70607776 | None:intergenic | 55.0% |
| ! | TGTCACTCCTTGGTGGTCAC+AGG | + | chr1.1:70606891-70606910 | MS.gene88891:intron | 55.0% |
| !! | CCAGGATTGGCTGTAGCAGA+TGG | + | chr1.1:70607315-70607334 | MS.gene88891:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70606699 | 70607936 | 70606699 | ID=MS.gene88891 |
| chr1.1 | mRNA | 70606699 | 70607936 | 70606699 | ID=MS.gene88891.t1;Parent=MS.gene88891 |
| chr1.1 | exon | 70606699 | 70606740 | 70606699 | ID=MS.gene88891.t1.exon1;Parent=MS.gene88891.t1 |
| chr1.1 | CDS | 70606699 | 70606740 | 70606699 | ID=cds.MS.gene88891.t1;Parent=MS.gene88891.t1 |
| chr1.1 | exon | 70607264 | 70607356 | 70607264 | ID=MS.gene88891.t1.exon2;Parent=MS.gene88891.t1 |
| chr1.1 | CDS | 70607264 | 70607356 | 70607264 | ID=cds.MS.gene88891.t1;Parent=MS.gene88891.t1 |
| chr1.1 | exon | 70607637 | 70607936 | 70607637 | ID=MS.gene88891.t1.exon3;Parent=MS.gene88891.t1 |
| chr1.1 | CDS | 70607637 | 70607936 | 70607637 | ID=cds.MS.gene88891.t1;Parent=MS.gene88891.t1 |
| Gene Sequence |
| Protein sequence |
