AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057249

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057249.t1 MTR_3g092780 94.298 684 14 3 1 659 1 684 0.0 1328
MS.gene057249.t1 MTR_3g092780 94.161 685 14 4 1 659 1 685 0.0 1323
MS.gene057249.t1 MTR_3g092780 88.158 684 15 4 1 659 1 643 0.0 1167
MS.gene057249.t1 MTR_3g092780 88.029 685 15 5 1 659 1 644 0.0 1162
MS.gene057249.t1 MTR_7g118260 38.961 616 322 16 47 647 7 583 1.86e-106 335
MS.gene057249.t1 MTR_1g067110 31.044 699 354 21 42 647 73 736 3.95e-67 234
MS.gene057249.t1 MTR_7g118260 71.212 132 38 0 47 178 7 138 8.93e-61 213
MS.gene057249.t1 MTR_7g118260 34.118 340 193 11 323 647 223 546 1.09e-30 127
MS.gene057249.t1 MTR_1g067110 29.161 679 353 21 42 627 73 716 1.13e-52 194
MS.gene057249.t1 MTR_4g061360 58.015 131 55 0 45 175 80 210 2.44e-48 182
MS.gene057249.t1 MTR_4g061360 76.000 50 12 0 598 647 714 763 1.01e-17 87.8
MS.gene057249.t1 MTR_7g118260 30.849 483 282 16 187 647 45 497 2.67e-34 137
MS.gene057249.t1 MTR_7g118260 34.118 340 193 11 323 647 90 413 8.63e-31 125
MS.gene057249.t1 MTR_3g037390 44.203 138 77 0 48 185 18 155 3.84e-30 125
MS.gene057249.t1 MTR_3g037390 68.889 45 14 0 605 649 498 542 1.53e-12 70.9
MS.gene057249.t1 MTR_4g108880 40.940 149 77 1 28 176 18 155 1.78e-29 124
MS.gene057249.t1 MTR_4g108880 73.333 45 12 0 605 649 505 549 5.90e-14 75.5
MS.gene057249.t1 MTR_1g098620 81.667 60 11 0 367 426 28 87 3.04e-26 103
MS.gene057249.t1 MTR_1g032570 42.276 123 66 2 54 175 15 133 6.40e-24 107
MS.gene057249.t1 MTR_3g086100 41.129 124 68 2 54 176 17 136 2.01e-23 105
MS.gene057249.t1 MTR_2g450070 37.302 126 74 2 54 178 22 143 2.13e-23 105
MS.gene057249.t1 MTR_2g450070 37.302 126 74 2 54 178 22 143 2.39e-23 105
MS.gene057249.t1 MTR_2g034960 36.943 157 93 3 54 208 33 185 9.79e-23 103
MS.gene057249.t1 MTR_4g131580 37.903 124 72 2 54 176 21 140 1.44e-22 102
MS.gene057249.t1 MTR_3g106220 31.677 161 98 3 54 213 12 161 1.48e-22 102
MS.gene057249.t1 MTR_1g013170 33.813 139 87 2 54 191 9 143 5.53e-22 100
MS.gene057249.t1 MTR_4g121020 35.669 157 95 3 54 208 35 187 5.79e-22 101
MS.gene057249.t1 MTR_4g121020 35.669 157 95 3 54 208 35 187 5.96e-22 101
MS.gene057249.t1 MTR_8g079940 35.088 171 96 5 54 214 29 194 2.38e-21 98.6
MS.gene057249.t1 MTR_4g131570 37.097 124 73 2 54 176 21 140 2.94e-21 98.6
MS.gene057249.t1 MTR_4g131600 36.290 124 74 2 54 176 24 143 7.09e-21 97.4
MS.gene057249.t1 MTR_3g102600 38.095 126 73 2 54 178 12 133 9.11e-21 90.9
MS.gene057249.t1 MTR_3g102600 33.140 172 97 4 54 224 12 166 1.91e-20 91.3
MS.gene057249.t1 MTR_1g013180 33.113 151 95 3 41 190 148 293 1.79e-19 93.2
MS.gene057249.t1 MTR_8g024260 73.333 60 10 1 599 652 578 637 1.11e-17 87.4
MS.gene057249.t1 MTR_7g117705 31.429 140 90 3 54 191 9 144 8.35e-17 84.0
MS.gene057249.t1 MTR_1g049100 30.435 138 85 1 54 180 8 145 1.21e-15 76.3
MS.gene057249.t1 MTR_7g490310 29.496 139 80 2 54 174 9 147 2.01e-15 76.6
MS.gene057249.t1 MTR_3g102600 44.304 79 39 2 101 178 10 84 3.60e-15 73.2
MS.gene057249.t1 MTR_5g036480 27.513 189 111 4 54 229 16 191 4.28e-15 75.9
MS.gene057249.t1 MTR_3g102590 31.707 164 105 3 54 216 12 169 1.40e-14 74.3
MS.gene057249.t1 MTR_3g102600 44.304 79 39 2 101 178 10 84 1.45e-14 72.8
MS.gene057249.t1 MTR_3g102590 31.138 167 108 3 54 219 12 172 1.63e-14 73.2
MS.gene057249.t1 MTR_1g067110 44.000 100 55 1 135 233 1 100 1.68e-14 77.4
MS.gene057249.t1 MTR_1g067110 37.589 141 69 4 526 647 431 571 3.78e-13 72.8
MS.gene057249.t1 MTR_3g078613 27.703 148 98 2 54 192 23 170 1.10e-13 70.9
MS.gene057249.t1 MTR_4g098870 31.034 145 91 4 42 185 8 144 2.08e-13 73.6
MS.gene057249.t1 MTR_4g098870 31.034 145 91 4 42 185 28 164 2.66e-13 73.6
MS.gene057249.t1 MTR_4g098870 31.034 145 91 4 42 185 8 144 3.16e-13 73.2
MS.gene057249.t1 MTR_4g098870 31.034 145 91 4 42 185 28 164 3.67e-13 73.2
MS.gene057249.t1 MTR_4g098870 31.034 145 91 4 42 185 47 183 4.39e-13 72.8
MS.gene057249.t1 MTR_3g037390 68.889 45 14 0 605 649 394 438 1.15e-12 71.2
MS.gene057249.t1 MTR_3g037390 69.048 42 13 0 135 176 1 42 1.43e-12 70.9
MS.gene057249.t1 MTR_1g032570 48.529 68 30 2 109 175 1 64 1.31e-12 71.2
MS.gene057249.t1 MTR_4g106590 29.771 131 82 2 54 174 26 156 2.56e-12 67.0
MS.gene057249.t1 MTR_3g078613 27.517 149 98 3 54 192 23 171 3.62e-12 66.2
MS.gene057249.t1 MTR_5g014040 29.167 144 93 4 42 184 8 143 7.14e-12 68.9
MS.gene057249.t1 MTR_5g014040 29.167 144 93 4 42 184 8 143 7.79e-12 68.6
MS.gene057249.t1 MTR_3g105710 50.769 65 31 1 592 655 219 283 8.16e-12 67.0
MS.gene057249.t1 MTR_1g013160 38.272 81 45 2 112 191 1 77 1.18e-11 63.9
MS.gene057249.t1 MTR_3g093860 31.746 126 71 3 56 168 20 143 1.32e-11 63.5
MS.gene057249.t1 MTR_8g038620 28.261 138 82 2 54 174 9 146 2.43e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057249.t1 AT5G24470 42.350 451 175 14 39 464 35 425 2.47e-93 300
MS.gene057249.t1 AT5G24470 68.657 67 11 2 587 647 489 551 1.02e-18 90.5
MS.gene057249.t1 AT2G46790 74.627 134 34 0 42 175 25 158 1.60e-68 231
MS.gene057249.t1 AT2G46790 74.627 134 34 0 42 175 25 158 2.33e-68 231
MS.gene057249.t1 AT2G46790 46.281 121 56 3 534 652 351 464 1.20e-17 86.7
MS.gene057249.t1 AT5G60100 60.448 134 53 0 42 175 52 185 5.94e-52 185
MS.gene057249.t1 AT5G60100 60.448 134 53 0 42 175 52 185 5.94e-52 185
MS.gene057249.t1 AT5G60100 60.448 134 53 0 42 175 52 185 5.94e-52 185
MS.gene057249.t1 AT5G60100 60.448 134 53 0 42 175 52 185 1.38e-51 186
MS.gene057249.t1 AT5G60100 69.841 63 18 1 596 658 433 494 3.90e-17 85.1
MS.gene057249.t1 AT5G60100 60.448 134 53 0 42 175 52 185 1.38e-51 186
MS.gene057249.t1 AT5G60100 69.841 63 18 1 596 658 433 494 3.90e-17 85.1
MS.gene057249.t1 AT5G60100 60.448 134 53 0 42 175 52 185 1.38e-51 186
MS.gene057249.t1 AT5G60100 69.841 63 18 1 596 658 433 494 3.90e-17 85.1
MS.gene057249.t1 AT5G60100 60.448 134 53 0 42 175 52 185 1.43e-50 184
MS.gene057249.t1 AT5G60100 69.841 63 18 1 596 658 460 521 5.22e-17 85.1
MS.gene057249.t1 AT5G02810 49.153 177 79 2 10 175 23 199 8.44e-48 179
MS.gene057249.t1 AT5G02810 83.721 43 7 0 605 647 669 711 8.83e-16 81.6
MS.gene057249.t1 AT5G61380 43.571 140 76 1 37 176 5 141 1.89e-29 124
MS.gene057249.t1 AT5G61380 68.889 45 14 0 605 649 533 577 1.27e-12 71.2
MS.gene057249.t1 AT2G25180 35.294 204 122 4 54 255 17 212 1.40e-24 108
MS.gene057249.t1 AT4G31920 40.164 122 68 2 54 174 17 134 1.28e-22 102
MS.gene057249.t1 AT3G16857 38.356 146 79 4 34 178 23 158 6.19e-22 101
MS.gene057249.t1 AT3G16857 38.356 146 79 4 34 178 23 158 6.36e-22 101
MS.gene057249.t1 AT2G01760 40.800 125 68 2 54 176 11 131 1.29e-20 94.7
MS.gene057249.t1 AT1G67710 38.710 124 71 2 54 176 11 130 3.03e-20 95.1
MS.gene057249.t1 AT2G46670 81.250 48 9 0 605 652 132 179 5.26e-19 85.9
MS.gene057249.t1 AT5G58080 33.333 150 92 3 44 193 10 151 1.98e-18 90.1
MS.gene057249.t1 AT2G46790 81.250 48 9 0 605 652 260 307 3.67e-18 86.3
MS.gene057249.t1 AT2G46790 81.250 48 9 0 605 652 260 307 3.67e-18 86.3
MS.gene057249.t1 AT2G46790 55.294 85 35 2 568 652 266 347 7.01e-18 86.3
MS.gene057249.t1 AT2G46790 80.488 41 8 0 135 175 1 41 4.40e-17 84.0
MS.gene057249.t1 AT2G01760 37.143 140 72 5 48 176 25 159 9.98e-17 83.6
MS.gene057249.t1 AT5G02810 83.721 43 7 0 605 647 525 567 5.65e-16 82.0
MS.gene057249.t1 AT5G02810 54.237 59 23 1 117 175 1 55 1.98e-13 73.9
MS.gene057249.t1 AT3G16857 40.659 91 49 2 89 178 2 88 1.20e-15 81.3
MS.gene057249.t1 AT1G19050 30.827 133 83 2 54 177 24 156 9.67e-15 73.9
MS.gene057249.t1 AT1G59940 32.836 134 81 1 56 180 35 168 1.37e-14 74.3
MS.gene057249.t1 AT3G62670 30.857 175 109 5 46 216 34 200 1.64e-14 76.6
MS.gene057249.t1 AT1G10470 32.031 128 78 1 56 174 36 163 2.31e-14 73.9
MS.gene057249.t1 AT1G10470 32.031 128 78 1 56 174 22 149 2.53e-14 73.6
MS.gene057249.t1 AT1G59940 32.836 134 81 1 56 180 127 260 3.70e-14 74.7
MS.gene057249.t1 AT3G48100 31.429 140 85 3 50 179 22 160 1.67e-13 69.7
MS.gene057249.t1 AT5G62920 26.829 164 111 1 38 192 9 172 2.25e-13 69.7
MS.gene057249.t1 AT3G57040 28.571 140 81 1 54 174 9 148 2.63e-13 70.5
MS.gene057249.t1 AT3G57040 28.571 140 81 1 54 174 9 148 2.63e-13 70.5
MS.gene057249.t1 AT1G74890 30.000 130 82 1 54 174 18 147 4.30e-13 69.3
MS.gene057249.t1 AT2G41310 30.435 138 79 2 54 174 9 146 8.31e-13 68.9
MS.gene057249.t1 AT5G49240 33.871 124 77 2 54 176 42 161 1.19e-11 66.6
MS.gene057249.t1 AT3G56380 28.358 134 86 2 54 177 20 153 1.27e-11 63.5
MS.gene057249.t1 AT1G49190 28.689 122 82 2 56 176 36 153 2.81e-11 67.0
MS.gene057249.t1 AT1G49190 28.689 122 82 2 56 176 36 153 3.84e-11 65.9

Find 124 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGAATCCCGATTCTCTTTC+TGG 0.070056 3.2:+78075991 MS.gene057249:CDS
CGCACTTCTCACATTAATTA+TGG 0.184807 3.2:+78073786 MS.gene057249:CDS
TACGAACCTCGCGGTCTAAA+TGG 0.244627 3.2:+78075465 MS.gene057249:CDS
TCTCTTCCGATTCCTGTAAA+AGG 0.269959 3.2:+78075273 MS.gene057249:CDS
GAGCAGCGTCCTCGAGTTAA+AGG 0.270096 3.2:+78075952 MS.gene057249:CDS
CAAGAACTTGACATTCATTT+AGG 0.270340 3.2:+78074386 MS.gene057249:CDS
TCCAGTAGTATTTGTAATAA+TGG 0.285223 3.2:+78075585 MS.gene057249:CDS
GGAATTTGTGGCAGCATGTT+TGG 0.301229 3.2:+78074034 MS.gene057249:CDS
GCCGCAACCGCTGGCATAAA+TGG 0.311050 3.2:+78074188 MS.gene057249:CDS
GCCATTTATGCCAGCGGTTG+CGG 0.331299 3.2:-78074189 None:intergenic
TGCTGTTGCTGATGGCTTGA+AGG 0.353593 3.2:+78073684 MS.gene057249:CDS
TGCTTGCATTCAAAGAAATA+AGG 0.359592 3.2:+78074295 MS.gene057249:CDS
AAGGAAGCTATTGACTTGAT+TGG 0.361168 3.2:+78074700 MS.gene057249:CDS
ACCAATGCTGGTAAAATTGC+TGG 0.363758 3.2:-78075613 None:intergenic
GTTCCTGTGTATACACAATT+TGG 0.365801 3.2:-78075503 None:intergenic
GTATTGAACTATCTGAGTTA+TGG 0.368410 3.2:-78075197 None:intergenic
CTTAGAAAATGCAACTATAA+AGG 0.375160 3.2:+78072856 MS.gene057249:CDS
TTCGAGGAACACAACTGAAC+CGG 0.382827 3.2:-78075379 None:intergenic
GAGATCCTTCTCAAAGTTGC+TGG 0.389986 3.2:-78074830 None:intergenic
TGTGTATACACAATTTGGTT+TGG 0.392077 3.2:-78075498 None:intergenic
ACAACTGAACCGGGCATTGA+TGG 0.393075 3.2:-78075369 None:intergenic
GATACACTTTGATCAGTTGT+AGG 0.394686 3.2:-78075564 None:intergenic
TCCATTATTACAAATACTAC+TGG 0.397236 3.2:-78075586 None:intergenic
GCGTATCACTTTCATCTTGA+GGG 0.403662 3.2:-78074211 None:intergenic
CAGAACAGCAGCAGTTTCAG+AGG 0.404213 3.2:+78075686 MS.gene057249:CDS
GAGAAGGTGAGGGAAGAAGA+AGG 0.408178 3.2:+78072694 MS.gene057249:CDS
CTTAGTTCATTTATTCGAAT+CGG 0.408502 3.2:-78074012 None:intergenic
CATAAGGGTGTGCCTTTCTT+CGG 0.412245 3.2:-78074854 None:intergenic
TCAAATTTACTCTACTACTA+AGG 0.413508 3.2:+78074681 MS.gene057249:CDS
GAGAAGTGCGTAGCCGGATA+TGG 0.417405 3.2:-78073773 None:intergenic
TGTCCTGTGCGATCACTGCC+TGG 0.424540 3.2:-78074409 None:intergenic
CTGAAGCATTGGAATGCATA+AGG 0.429243 3.2:-78074870 None:intergenic
AATGATCTTTGTATGGCCTA+TGG 0.430478 3.2:+78075306 MS.gene057249:CDS
GTCGATTTGCCAGCCATATC+CGG 0.434584 3.2:+78073760 MS.gene057249:CDS
CGATTCCTGTAAAAGGTGTA+AGG 0.438701 3.2:+78075280 MS.gene057249:CDS
CCAGAAAGAGAATCGGGATT+CGG 0.439697 3.2:-78075991 None:intergenic
TTCAATATCATACTTATGAC+AGG 0.442500 3.2:-78073832 None:intergenic
TCTTGTGTCTTTATTTCACT+TGG 0.446311 3.2:-78074368 None:intergenic
GCAGTTGCTGCTGTTGCTGA+TGG 0.454486 3.2:+78073676 MS.gene057249:intron
AGAGAGATGTTATGAAAAGA+AGG 0.455803 3.2:+78075797 MS.gene057249:CDS
CCAGGGTTTCGTACACAATC+AGG 0.457218 3.2:+78075342 MS.gene057249:CDS
AATAAGGACTTAATTGAGAA+AGG 0.462433 3.2:+78074311 MS.gene057249:CDS
AAGGTTAAATGATCTTTGTA+TGG 0.462879 3.2:+78075299 MS.gene057249:CDS
CAGCAGTCAGCCGCAACCGC+TGG 0.465375 3.2:+78074179 MS.gene057249:intron
GTGAAATGAAAGGGCTTATG+AGG 0.465706 3.2:+78072749 MS.gene057249:CDS
GTTTGACCATTCTCCACAGT+TGG 0.467281 3.2:+78074783 MS.gene057249:CDS
TTGCTGATGGCTTGAAGGCA+TGG 0.467739 3.2:+78073689 MS.gene057249:CDS
ATTCATGCAGGTATACAAAC+AGG 0.476773 3.2:+78075100 MS.gene057249:intron
TAAATGAACTAAGGAATTTG+TGG 0.487302 3.2:+78074022 MS.gene057249:CDS
ATACACTTTGATCAGTTGTA+GGG 0.491753 3.2:-78075563 None:intergenic
GAGAAGTTAATTACTACTGC+AGG 0.495962 3.2:-78075138 None:intergenic
AGTGGAAGAAGAAGAAGAGA+AGG 0.496753 3.2:+78072678 MS.gene057249:CDS
TGCTGATGGCTTGAAGGCAT+GGG 0.496945 3.2:+78073690 MS.gene057249:CDS
AATTCATCAATTGATTGCAC+AGG 0.498212 3.2:+78074757 MS.gene057249:CDS
CCGTTTGAAAGCTGAAGCAT+TGG 0.498244 3.2:-78074881 None:intergenic
TAATGTGAGAAGTGCGTAGC+CGG 0.502440 3.2:-78073779 None:intergenic
AGAAGGTGAGGGAAGAAGAA+GGG 0.503650 3.2:+78072695 MS.gene057249:CDS
GTGAGGGAAGAAGAAGGGAG+TGG 0.503857 3.2:+78072700 MS.gene057249:CDS
TGGTGAGAAAATAGTGAGAG+TGG 0.512691 3.2:+78072660 MS.gene057249:CDS
CCAATGCTTCAGCTTTCAAA+CGG 0.514454 3.2:+78074881 MS.gene057249:CDS
GGAACACAAGTCAGAACATC+CGG 0.514906 3.2:+78075521 MS.gene057249:CDS
TGCGTATCACTTTCATCTTG+AGG 0.515709 3.2:-78074212 None:intergenic
AATGAAGAACTCTCAAACAG+TGG 0.522421 3.2:+78075714 MS.gene057249:CDS
ATTACTTCCATAACCAATGC+TGG 0.523079 3.2:-78075625 None:intergenic
AATGAAAGGGCTTATGAGGT+GGG 0.528311 3.2:+78072753 MS.gene057249:CDS
TGAAGCATTGGAATGCATAA+GGG 0.528347 3.2:-78074869 None:intergenic
GGTGGTGGTGGTGAAATGAA+AGG 0.529163 3.2:+78072739 MS.gene057249:CDS
GAGATAAGTCTTTGATCCTC+CGG 0.529482 3.2:-78075540 None:intergenic
GAGAGTAGAGGTGGTGGTGG+TGG 0.529593 3.2:+78072727 MS.gene057249:CDS
AGTGCGTAGCCGGATATGGC+TGG 0.534736 3.2:-78073769 None:intergenic
AAAGATCCAACTGTGGAGAA+TGG 0.536900 3.2:-78074789 None:intergenic
TGGTGGAAGTGTTGAACCAT+AGG 0.536921 3.2:-78075322 None:intergenic
GTGGTGGTGGTGAAATGAAA+GGG 0.545693 3.2:+78072740 MS.gene057249:CDS
AGTGGAACTGAGAGTAGAGG+TGG 0.546233 3.2:+78072718 MS.gene057249:CDS
ATTCACATCGAGCTATGCTA+CGG 0.546537 3.2:+78075739 MS.gene057249:CDS
AGAAGGATCTCACCGAAGAA+AGG 0.548442 3.2:+78074842 MS.gene057249:CDS
AGATCCTTCTCAAAGTTGCT+GGG 0.549454 3.2:-78074829 None:intergenic
GGGAGTGGAACTGAGAGTAG+AGG 0.550599 3.2:+78072715 MS.gene057249:CDS
AAGAAGAAGAAGAGAAGGTG+AGG 0.553208 3.2:+78072683 MS.gene057249:CDS
ACTGAGAGTAGAGGTGGTGG+TGG 0.553345 3.2:+78072724 MS.gene057249:CDS
AACGCTGCTAGTAATCGTTC+CGG 0.555367 3.2:+78074263 MS.gene057249:CDS
TTTAGGAATGCTAGCATCAG+TGG 0.555975 3.2:+78074634 MS.gene057249:CDS
AGAAGAAGAAGAGAAGGTGA+GGG 0.557623 3.2:+78072684 MS.gene057249:CDS
CGATTCGAATAAATGAACTA+AGG 0.559225 3.2:+78074013 MS.gene057249:CDS
AAATGAAAGGGCTTATGAGG+TGG 0.560859 3.2:+78072752 MS.gene057249:CDS
TCGAGGAACACAACTGAACC+GGG 0.562948 3.2:-78075378 None:intergenic
ACTTGACATTCATTTAGGCC+AGG 0.566880 3.2:+78074391 MS.gene057249:CDS
AGAACAGCAGCAGTTTCAGA+GGG 0.568196 3.2:+78075687 MS.gene057249:CDS
CAACTTCACATTCTCAGCAA+GGG 0.576831 3.2:+78075247 MS.gene057249:CDS
GGAACTGAGAGTAGAGGTGG+TGG 0.580734 3.2:+78072721 MS.gene057249:CDS
GCAACTTCACATTCTCAGCA+AGG 0.583947 3.2:+78075246 MS.gene057249:CDS
TCAGGTCCACCATCAATGCC+CGG 0.589208 3.2:+78075360 MS.gene057249:CDS
AGCGCAAATAATTGCAAGGA+CGG 0.590278 3.2:+78074595 MS.gene057249:CDS
TCATCCCAGCAACTTTGAGA+AGG 0.593867 3.2:+78074825 MS.gene057249:CDS
ACTGAACCGGGCATTGATGG+TGG 0.596008 3.2:-78075366 None:intergenic
ATTGGTTATGGAAGTAATTG+CGG 0.597392 3.2:+78075630 MS.gene057249:CDS
TTCACATCGAGCTATGCTAC+GGG 0.598642 3.2:+78075740 MS.gene057249:CDS
AAAGAGAATCGGGATTCGGT+TGG 0.599964 3.2:-78075987 None:intergenic
TATGGTTCAACACTTCCACC+AGG 0.607254 3.2:+78075324 MS.gene057249:CDS
GACAAGCGCAAATAATTGCA+AGG 0.612224 3.2:+78074591 MS.gene057249:CDS
GGAAGTAATTGCGGAAGTAG+CGG 0.613525 3.2:+78075639 MS.gene057249:CDS
AATGCAAGCAGCATCACCGC+CGG 0.616301 3.2:-78074282 None:intergenic
ACAAATTGTCCTTTAACTCG+AGG 0.617609 3.2:-78075961 None:intergenic
TTGAGGGCCATTTATGCCAG+CGG 0.618633 3.2:-78074195 None:intergenic
AATAAACAACAACATGGGTG+AGG 0.620435 3.2:+78072627 None:intergenic
ATCGCACAGGACAGTCACGC+CGG 0.625686 3.2:+78074419 MS.gene057249:CDS
CCTGATTGTGTACGAAACCC+TGG 0.626598 3.2:-78075342 None:intergenic
AAGGCATGGGAAATACTCAA+AGG 0.628269 3.2:+78073703 MS.gene057249:CDS
TACACTTTGATCAGTTGTAG+GGG 0.630188 3.2:-78075562 None:intergenic
AAACCAAATTGTGTATACAC+AGG 0.639133 3.2:+78075500 MS.gene057249:CDS
AGGCCAGGCAGTGATCGCAC+AGG 0.641121 3.2:+78074406 MS.gene057249:CDS
GAATGCTAGCATCAGTGGTG+AGG 0.644176 3.2:+78074639 MS.gene057249:CDS
GTATACAAATGCATGTTGAG+AGG 0.663604 3.2:+78073967 MS.gene057249:CDS
CTGTTTCCATTTAGACCGCG+AGG 0.673170 3.2:-78075471 None:intergenic
ATGGTTCAACACTTCCACCA+GGG 0.675718 3.2:+78075325 MS.gene057249:CDS
GCTGCTAGTAATCGTTCCGG+CGG 0.691660 3.2:+78074266 MS.gene057249:CDS
TCAATATCATACTTATGACA+GGG 0.693313 3.2:-78073831 None:intergenic
ATGGGTGAGGTTGTAATGAG+TGG 0.695889 3.2:+78072640 MS.gene057249:CDS
CTGAGAGAAAGATCCAACTG+TGG 0.713368 3.2:-78074796 None:intergenic
GCACAGGACAGTCACGCCGG+AGG 0.715159 3.2:+78074422 MS.gene057249:CDS
GAGCAGCTTTACGAACCTCG+CGG 0.732658 3.2:+78075456 MS.gene057249:CDS
ACACAAGTCAGAACATCCGG+AGG 0.742561 3.2:+78075524 MS.gene057249:CDS
GATTGTGTACGAAACCCTGG+TGG 0.767923 3.2:-78075339 None:intergenic
AGTATCAGATCAATACTGCG+CGG 0.783873 3.2:-78073730 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATTTATTAACAAAATT+GGG + chr3.2:78074125-78074144 MS.gene057249:intron 10.0%
!! TAAGAATTTATTAACAAAAT+TGG + chr3.2:78074124-78074143 MS.gene057249:intron 10.0%
!! TAATAATTACAAAAATTAAG+GGG + chr3.2:78073286-78073305 MS.gene057249:intron 10.0%
!! ACGAAAAAATTAGAATAAAA+AGG - chr3.2:78074920-78074939 None:intergenic 15.0%
!! CGAAAAAATTAGAATAAAAA+GGG - chr3.2:78074919-78074938 None:intergenic 15.0%
!!! ACTTATTTATACTTTTTAAC+AGG + chr3.2:78075900-78075919 MS.gene057249:intron 15.0%
!!! ATTATTGAGTTTTATTATCT+TGG + chr3.2:78073567-78073586 MS.gene057249:intron 15.0%
!!! TTGAACAAACTTTTATTTTT+AGG + chr3.2:78074508-78074527 MS.gene057249:intron 15.0%
!! ATTAGAAATTTAGAAGTCTT+TGG + chr3.2:78073198-78073217 MS.gene057249:intron 20.0%
!! GTAAATATTAGGTTATATTG+AGG + chr3.2:78073103-78073122 MS.gene057249:intron 20.0%
! AAGAACTTCAAAACATGATA+CGG + chr3.2:78074983-78075002 MS.gene057249:intron 25.0%
! AAGGTTAAATGATCTTTGTA+TGG + chr3.2:78075299-78075318 MS.gene057249:CDS 25.0%
! AATAAGGACTTAATTGAGAA+AGG + chr3.2:78074311-78074330 MS.gene057249:CDS 25.0%
! AATATTGCTGTGTGTTTAAT+TGG + chr3.2:78072931-78072950 MS.gene057249:intron 25.0%
! AATTACAAAAATTAAGGGGA+TGG + chr3.2:78073290-78073309 MS.gene057249:intron 25.0%
! AGCACAATTAACTGTAAAAA+TGG + chr3.2:78074565-78074584 MS.gene057249:CDS 25.0%
! CTTAGAAAATGCAACTATAA+AGG + chr3.2:78072856-78072875 MS.gene057249:CDS 25.0%
! CTTAGTTCATTTATTCGAAT+CGG - chr3.2:78074015-78074034 None:intergenic 25.0%
! GAATAATTGTACCAAGTTTA+TGG - chr3.2:78073520-78073539 None:intergenic 25.0%
! TAAATGAACTAAGGAATTTG+TGG + chr3.2:78074022-78074041 MS.gene057249:CDS 25.0%
! TAGAACTTAGATTTACTTTC+AGG - chr3.2:78073626-78073645 None:intergenic 25.0%
! TCAAATTTACTCTACTACTA+AGG + chr3.2:78074681-78074700 MS.gene057249:CDS 25.0%
! TCAATATCATACTTATGACA+GGG - chr3.2:78073834-78073853 None:intergenic 25.0%
! TCCAGTAGTATTTGTAATAA+TGG + chr3.2:78075585-78075604 MS.gene057249:CDS 25.0%
! TCCATTATTACAAATACTAC+TGG - chr3.2:78075589-78075608 None:intergenic 25.0%
! TTCAATATCATACTTATGAC+AGG - chr3.2:78073835-78073854 None:intergenic 25.0%
!!! TTTATTTTTAGGATTAAGCG+TGG + chr3.2:78074519-78074538 MS.gene057249:intron 25.0%
!!! TTTTTTAAAGAGAAGTGACA+TGG + chr3.2:78073414-78073433 MS.gene057249:intron 25.0%
AAACCAAATTGTGTATACAC+AGG + chr3.2:78075500-78075519 MS.gene057249:CDS 30.0%
AATTCATCAATTGATTGCAC+AGG + chr3.2:78074757-78074776 MS.gene057249:CDS 30.0%
AGAGAGATGTTATGAAAAGA+AGG + chr3.2:78075797-78075816 MS.gene057249:CDS 30.0%
ATACACTTTGATCAGTTGTA+GGG - chr3.2:78075566-78075585 None:intergenic 30.0%
ATTGGTTATGGAAGTAATTG+CGG + chr3.2:78075630-78075649 MS.gene057249:CDS 30.0%
CAAGAACTTGACATTCATTT+AGG + chr3.2:78074386-78074405 MS.gene057249:CDS 30.0%
CGATTCGAATAAATGAACTA+AGG + chr3.2:78074013-78074032 MS.gene057249:CDS 30.0%
GAAAGAAAGAAATTTACCTC+CGG - chr3.2:78074441-78074460 None:intergenic 30.0%
GTATTGAACTATCTGAGTTA+TGG - chr3.2:78075200-78075219 None:intergenic 30.0%
TGCTATAAGACTCTATTTCA+AGG - chr3.2:78073475-78073494 None:intergenic 30.0%
TGCTTGCATTCAAAGAAATA+AGG + chr3.2:78074295-78074314 MS.gene057249:CDS 30.0%
TGTGTATACACAATTTGGTT+TGG - chr3.2:78075501-78075520 None:intergenic 30.0%
! AATAGAGTCTTATAGCAGTA+TGG + chr3.2:78073478-78073497 MS.gene057249:intron 30.0%
! ATCTCTCTTTTCGTTTCAAA+CGG - chr3.2:78075785-78075804 None:intergenic 30.0%
! GTGTATGATTTTTATGCTTG+TGG + chr3.2:78075019-78075038 MS.gene057249:intron 30.0%
! TCAGATTCTTTTTGCATACT+TGG - chr3.2:78075222-78075241 None:intergenic 30.0%
! TCTTGTGTCTTTATTTCACT+TGG - chr3.2:78074371-78074390 None:intergenic 30.0%
! TTTAACCTTACACCTTTTAC+AGG - chr3.2:78075288-78075307 None:intergenic 30.0%
! TTTTGTTAGCTGAGAATCTT+AGG + chr3.2:78073438-78073457 MS.gene057249:intron 30.0%
! TTTTTCTCCAGAAAGAGAAT+CGG - chr3.2:78076001-78076020 None:intergenic 30.0%
!!! AATTTTACCAGCATTGGTTA+TGG + chr3.2:78075618-78075637 MS.gene057249:CDS 30.0%
!!! GAATTTTTGAGACTGCAATT+CGG - chr3.2:78074742-78074761 None:intergenic 30.0%
AATGAAGAACTCTCAAACAG+TGG + chr3.2:78075714-78075733 MS.gene057249:CDS 35.0%
AATGATCTTTGTATGGCCTA+TGG + chr3.2:78075306-78075325 MS.gene057249:CDS 35.0%
ACAAATTGTCCTTTAACTCG+AGG - chr3.2:78075964-78075983 None:intergenic 35.0%
ATTACTTCCATAACCAATGC+TGG - chr3.2:78075628-78075647 None:intergenic 35.0%
ATTCATGCAGGTATACAAAC+AGG + chr3.2:78075100-78075119 MS.gene057249:intron 35.0%
CGCACTTCTCACATTAATTA+TGG + chr3.2:78073786-78073805 MS.gene057249:CDS 35.0%
GAGAAGTTAATTACTACTGC+AGG - chr3.2:78075141-78075160 None:intergenic 35.0%
GATACACTTTGATCAGTTGT+AGG - chr3.2:78075567-78075586 None:intergenic 35.0%
GGAGAAATTCTTGCCAAAAA+TGG + chr3.2:78072774-78072793 MS.gene057249:CDS 35.0%
GTATACAAATGCATGTTGAG+AGG + chr3.2:78073967-78073986 MS.gene057249:CDS 35.0%
GTTCCTGTGTATACACAATT+TGG - chr3.2:78075506-78075525 None:intergenic 35.0%
TACACTTTGATCAGTTGTAG+GGG - chr3.2:78075565-78075584 None:intergenic 35.0%
TGTTTGTATACCTGCATGAA+TGG - chr3.2:78075101-78075120 None:intergenic 35.0%
! ACTAGCTTAAGCCATAAACT+TGG + chr3.2:78073506-78073525 MS.gene057249:intron 35.0%
! AGTCTTTGGACTGCAAATTT+TGG + chr3.2:78073212-78073231 MS.gene057249:intron 35.0%
! CTTACACCTTTTACAGGAAT+CGG - chr3.2:78075282-78075301 None:intergenic 35.0%
! TTTTCTCCAGAAAGAGAATC+GGG - chr3.2:78076000-78076019 None:intergenic 35.0%
!! AAGGAAGCTATTGACTTGAT+TGG + chr3.2:78074700-78074719 MS.gene057249:CDS 35.0%
!! GCAAATTTTGGAAAGGATCA+AGG + chr3.2:78073224-78073243 MS.gene057249:intron 35.0%
!! TCTTGCCAAAAATGGTTTTG+AGG + chr3.2:78072782-78072801 MS.gene057249:CDS 35.0%
!! TGAAGCATTGGAATGCATAA+GGG - chr3.2:78074872-78074891 None:intergenic 35.0%
!! TTGGACTGCAAATTTTGGAA+AGG + chr3.2:78073217-78073236 MS.gene057249:intron 35.0%
!!! CTTGCCAAAAATGGTTTTGA+GGG + chr3.2:78072783-78072802 MS.gene057249:CDS 35.0%
AAAGATCCAACTGTGGAGAA+TGG - chr3.2:78074792-78074811 None:intergenic 40.0%
AAATGAAAGGGCTTATGAGG+TGG + chr3.2:78072752-78072771 MS.gene057249:CDS 40.0%
AAATGGTGTTCCATTCATGC+AGG + chr3.2:78075088-78075107 MS.gene057249:intron 40.0%
AAGAAGAAGAAGAGAAGGTG+AGG + chr3.2:78072683-78072702 MS.gene057249:CDS 40.0%
AAGGCATGGGAAATACTCAA+AGG + chr3.2:78073703-78073722 MS.gene057249:CDS 40.0%
AATGAAAGGGCTTATGAGGT+GGG + chr3.2:78072753-78072772 MS.gene057249:CDS 40.0%
ACCAATGCTGGTAAAATTGC+TGG - chr3.2:78075616-78075635 None:intergenic 40.0%
ACTTGACATTCATTTAGGCC+AGG + chr3.2:78074391-78074410 MS.gene057249:CDS 40.0%
AGAAGAAGAAGAGAAGGTGA+GGG + chr3.2:78072684-78072703 MS.gene057249:CDS 40.0%
AGATCCTTCTCAAAGTTGCT+GGG - chr3.2:78074832-78074851 None:intergenic 40.0%
AGCGCAAATAATTGCAAGGA+CGG + chr3.2:78074595-78074614 MS.gene057249:CDS 40.0%
AGTATCAGATCAATACTGCG+CGG - chr3.2:78073733-78073752 None:intergenic 40.0%
AGTGGAAGAAGAAGAAGAGA+AGG + chr3.2:78072678-78072697 MS.gene057249:CDS 40.0%
ATTCACATCGAGCTATGCTA+CGG + chr3.2:78075739-78075758 MS.gene057249:CDS 40.0%
CAACTTCACATTCTCAGCAA+GGG + chr3.2:78075247-78075266 MS.gene057249:CDS 40.0%
CCAATGCTTCAGCTTTCAAA+CGG + chr3.2:78074881-78074900 MS.gene057249:CDS 40.0%
CTGCATGCTTATCACTGAAA+TGG + chr3.2:78075071-78075090 MS.gene057249:intron 40.0%
GACAAGCGCAAATAATTGCA+AGG + chr3.2:78074591-78074610 MS.gene057249:CDS 40.0%
GAGATAAGTCTTTGATCCTC+CGG - chr3.2:78075543-78075562 None:intergenic 40.0%
GCATGCAGAATTCAACAAAG+CGG - chr3.2:78075059-78075078 None:intergenic 40.0%
GCGTATCACTTTCATCTTGA+GGG - chr3.2:78074214-78074233 None:intergenic 40.0%
GTGAAATGAAAGGGCTTATG+AGG + chr3.2:78072749-78072768 MS.gene057249:CDS 40.0%
GTTTGGAGAAGACAATCAGT+AGG + chr3.2:78074051-78074070 MS.gene057249:intron 40.0%
TCTCTTCCGATTCCTGTAAA+AGG + chr3.2:78075273-78075292 MS.gene057249:CDS 40.0%
TGCGTATCACTTTCATCTTG+AGG - chr3.2:78074215-78074234 None:intergenic 40.0%
TGGTGAGAAAATAGTGAGAG+TGG + chr3.2:78072660-78072679 MS.gene057249:CDS 40.0%
TTTAGGAATGCTAGCATCAG+TGG + chr3.2:78074634-78074653 MS.gene057249:CDS 40.0%
! AGCACCCTCAAAACCATTTT+TGG - chr3.2:78072790-78072809 None:intergenic 40.0%
! CGATTCCTGTAAAAGGTGTA+AGG + chr3.2:78075280-78075299 MS.gene057249:CDS 40.0%
!! ATCATTTTGTTCGACTCGCA+AGG + chr3.2:78074469-78074488 MS.gene057249:intron 40.0%
!! CTGAAGCATTGGAATGCATA+AGG - chr3.2:78074873-78074892 None:intergenic 40.0%
!!! AATGGTTTTGAGGGTGCTTT+TGG + chr3.2:78072792-78072811 MS.gene057249:CDS 40.0%
!!! CTGCTTGAAAGTTTTGTTCG+AGG - chr3.2:78075398-78075417 None:intergenic 40.0%
!!! GCGATGATCAAGAGCATTTT+AGG + chr3.2:78074617-78074636 MS.gene057249:CDS 40.0%
AACGCTGCTAGTAATCGTTC+CGG + chr3.2:78074263-78074282 MS.gene057249:CDS 45.0%
AGAACAGCAGCAGTTTCAGA+GGG + chr3.2:78075687-78075706 MS.gene057249:CDS 45.0%
AGAAGGATCTCACCGAAGAA+AGG + chr3.2:78074842-78074861 MS.gene057249:CDS 45.0%
AGAAGGTGAGGGAAGAAGAA+GGG + chr3.2:78072695-78072714 MS.gene057249:CDS 45.0%
AGCGTGGCTAACTGCAAAAA+TGG + chr3.2:78074535-78074554 MS.gene057249:CDS 45.0%
ATGGGTGAGGTTGTAATGAG+TGG + chr3.2:78072640-78072659 MS.gene057249:CDS 45.0%
ATGGTTCAACACTTCCACCA+GGG + chr3.2:78075325-78075344 MS.gene057249:CDS 45.0%
CCAGAAAGAGAATCGGGATT+CGG - chr3.2:78075994-78076013 None:intergenic 45.0%
CCGTTTGAAAGCTGAAGCAT+TGG - chr3.2:78074884-78074903 None:intergenic 45.0%
CTGAGAGAAAGATCCAACTG+TGG - chr3.2:78074799-78074818 None:intergenic 45.0%
GAGATCCTTCTCAAAGTTGC+TGG - chr3.2:78074833-78074852 None:intergenic 45.0%
GCAACTTCACATTCTCAGCA+AGG + chr3.2:78075246-78075265 MS.gene057249:CDS 45.0%
GGAACACAAGTCAGAACATC+CGG + chr3.2:78075521-78075540 MS.gene057249:CDS 45.0%
GGAAGTAATTGCGGAAGTAG+CGG + chr3.2:78075639-78075658 MS.gene057249:CDS 45.0%
GGAATTTGTGGCAGCATGTT+TGG + chr3.2:78074034-78074053 MS.gene057249:CDS 45.0%
GGCTAACTGCAAAAATGGAG+TGG + chr3.2:78074540-78074559 MS.gene057249:CDS 45.0%
GTTTGACCATTCTCCACAGT+TGG + chr3.2:78074783-78074802 MS.gene057249:CDS 45.0%
TATGGTTCAACACTTCCACC+AGG + chr3.2:78075324-78075343 MS.gene057249:CDS 45.0%
TGGAAAGGATCAAGGCTCAA+GGG + chr3.2:78073232-78073251 MS.gene057249:intron 45.0%
TTCACATCGAGCTATGCTAC+GGG + chr3.2:78075740-78075759 MS.gene057249:CDS 45.0%
TTCGAGGAACACAACTGAAC+CGG - chr3.2:78075382-78075401 None:intergenic 45.0%
TTGGAAAGGATCAAGGCTCA+AGG + chr3.2:78073231-78073250 MS.gene057249:intron 45.0%
! AAAGAGAATCGGGATTCGGT+TGG - chr3.2:78075990-78076009 None:intergenic 45.0%
! CATAAGGGTGTGCCTTTCTT+CGG - chr3.2:78074857-78074876 None:intergenic 45.0%
! GCCAGCAATTTTACCAGCAT+TGG + chr3.2:78075612-78075631 MS.gene057249:CDS 45.0%
! TAATGTGAGAAGTGCGTAGC+CGG - chr3.2:78073782-78073801 None:intergenic 45.0%
! TCATCCCAGCAACTTTGAGA+AGG + chr3.2:78074825-78074844 MS.gene057249:CDS 45.0%
!! GTGGTGGTGGTGAAATGAAA+GGG + chr3.2:78072740-78072759 MS.gene057249:CDS 45.0%
!! TGGTGGAAGTGTTGAACCAT+AGG - chr3.2:78075325-78075344 None:intergenic 45.0%
!! AATTAATTTAAGTATTAATA+AGG - chr3.2:78073890-78073909 None:intergenic 5.0%
!! TTAATAATTACAAAAATTAA+GGG + chr3.2:78073285-78073304 MS.gene057249:intron 5.0%
!! TTTAATAATTACAAAAATTA+AGG + chr3.2:78073284-78073303 MS.gene057249:intron 5.0%
!! TTTAATTAATTGTAAATATT+AGG + chr3.2:78073092-78073111 MS.gene057249:intron 5.0%
ACACAAGTCAGAACATCCGG+AGG + chr3.2:78075524-78075543 MS.gene057249:CDS 50.0%
AGTGGAACTGAGAGTAGAGG+TGG + chr3.2:78072718-78072737 MS.gene057249:CDS 50.0%
CAGAACAGCAGCAGTTTCAG+AGG + chr3.2:78075686-78075705 MS.gene057249:CDS 50.0%
CCAGGGTTTCGTACACAATC+AGG + chr3.2:78075342-78075361 MS.gene057249:CDS 50.0%
CCGAATCCCGATTCTCTTTC+TGG + chr3.2:78075991-78076010 MS.gene057249:CDS 50.0%
CCTGATTGTGTACGAAACCC+TGG - chr3.2:78075345-78075364 None:intergenic 50.0%
CTGTTTCCATTTAGACCGCG+AGG - chr3.2:78075474-78075493 None:intergenic 50.0%
GAATCTTGCGACGACATCAC+TGG - chr3.2:78073937-78073956 None:intergenic 50.0%
GAATGCTAGCATCAGTGGTG+AGG + chr3.2:78074639-78074658 MS.gene057249:CDS 50.0%
GAGAAGGTGAGGGAAGAAGA+AGG + chr3.2:78072694-78072713 MS.gene057249:CDS 50.0%
GATTGTGTACGAAACCCTGG+TGG - chr3.2:78075342-78075361 None:intergenic 50.0%
TACGAACCTCGCGGTCTAAA+TGG + chr3.2:78075465-78075484 MS.gene057249:CDS 50.0%
TCGAGGAACACAACTGAACC+GGG - chr3.2:78075381-78075400 None:intergenic 50.0%
TGCTGTTGCTGATGGCTTGA+AGG + chr3.2:78073684-78073703 MS.gene057249:CDS 50.0%
TTGAGGGCCATTTATGCCAG+CGG - chr3.2:78074198-78074217 None:intergenic 50.0%
TTGCTGATGGCTTGAAGGCA+TGG + chr3.2:78073689-78073708 MS.gene057249:CDS 50.0%
! TGCTGATGGCTTGAAGGCAT+GGG + chr3.2:78073690-78073709 MS.gene057249:CDS 50.0%
!! ACAACTGAACCGGGCATTGA+TGG - chr3.2:78075372-78075391 None:intergenic 50.0%
!! AGCAGCGTTGTTTTCAGCAG+TGG - chr3.2:78074252-78074271 None:intergenic 50.0%
!! GGTGGTGGTGGTGAAATGAA+AGG + chr3.2:78072739-78072758 MS.gene057249:CDS 50.0%
!! GTCGATTTGCCAGCCATATC+CGG + chr3.2:78073760-78073779 MS.gene057249:CDS 50.0%
AATGCAAGCAGCATCACCGC+CGG - chr3.2:78074285-78074304 None:intergenic 55.0%
GAGCAGCGTCCTCGAGTTAA+AGG + chr3.2:78075952-78075971 MS.gene057249:CDS 55.0%
GAGCAGCTTTACGAACCTCG+CGG + chr3.2:78075456-78075475 MS.gene057249:CDS 55.0%
GCAGTTGCTGCTGTTGCTGA+TGG + chr3.2:78073676-78073695 MS.gene057249:intron 55.0%
GCCATTTATGCCAGCGGTTG+CGG - chr3.2:78074192-78074211 None:intergenic 55.0%
GCTGCTAGTAATCGTTCCGG+CGG + chr3.2:78074266-78074285 MS.gene057249:CDS 55.0%
GGAACTGAGAGTAGAGGTGG+TGG + chr3.2:78072721-78072740 MS.gene057249:CDS 55.0%
GGGAGTGGAACTGAGAGTAG+AGG + chr3.2:78072715-78072734 MS.gene057249:CDS 55.0%
GTGAGGGAAGAAGAAGGGAG+TGG + chr3.2:78072700-78072719 MS.gene057249:CDS 55.0%
TCAGGTCCACCATCAATGCC+CGG + chr3.2:78075360-78075379 MS.gene057249:CDS 55.0%
! ACTGAGAGTAGAGGTGGTGG+TGG + chr3.2:78072724-78072743 MS.gene057249:CDS 55.0%
! GAGAAGTGCGTAGCCGGATA+TGG - chr3.2:78073776-78073795 None:intergenic 55.0%
!! ACTGAACCGGGCATTGATGG+TGG - chr3.2:78075369-78075388 None:intergenic 55.0%
AGTGCGTAGCCGGATATGGC+TGG - chr3.2:78073772-78073791 None:intergenic 60.0%
ATCGCACAGGACAGTCACGC+CGG + chr3.2:78074419-78074438 MS.gene057249:CDS 60.0%
GCCGCAACCGCTGGCATAAA+TGG + chr3.2:78074188-78074207 MS.gene057249:CDS 60.0%
TGTCCTGTGCGATCACTGCC+TGG - chr3.2:78074412-78074431 None:intergenic 60.0%
!! GAGAGTAGAGGTGGTGGTGG+TGG + chr3.2:78072727-78072746 MS.gene057249:CDS 60.0%
AGGCCAGGCAGTGATCGCAC+AGG + chr3.2:78074406-78074425 MS.gene057249:CDS 65.0%
CAGCAGTCAGCCGCAACCGC+TGG + chr3.2:78074179-78074198 MS.gene057249:intron 70.0%
GCACAGGACAGTCACGCCGG+AGG + chr3.2:78074422-78074441 MS.gene057249:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 78072640 78076029 78072640 ID=MS.gene057249
chr3.2 mRNA 78072640 78076029 78072640 ID=MS.gene057249.t1;Parent=MS.gene057249
chr3.2 exon 78072640 78072877 78072640 ID=MS.gene057249.t1.exon1;Parent=MS.gene057249.t1
chr3.2 CDS 78072640 78072877 78072640 ID=cds.MS.gene057249.t1;Parent=MS.gene057249.t1
chr3.2 exon 78073680 78073841 78073680 ID=MS.gene057249.t1.exon2;Parent=MS.gene057249.t1
chr3.2 CDS 78073680 78073841 78073680 ID=cds.MS.gene057249.t1;Parent=MS.gene057249.t1
chr3.2 exon 78073938 78074068 78073938 ID=MS.gene057249.t1.exon3;Parent=MS.gene057249.t1
chr3.2 CDS 78073938 78074068 78073938 ID=cds.MS.gene057249.t1;Parent=MS.gene057249.t1
chr3.2 exon 78074185 78074443 78074185 ID=MS.gene057249.t1.exon4;Parent=MS.gene057249.t1
chr3.2 CDS 78074185 78074443 78074185 ID=cds.MS.gene057249.t1;Parent=MS.gene057249.t1
chr3.2 exon 78074530 78074902 78074530 ID=MS.gene057249.t1.exon5;Parent=MS.gene057249.t1
chr3.2 CDS 78074530 78074902 78074530 ID=cds.MS.gene057249.t1;Parent=MS.gene057249.t1
chr3.2 exon 78075110 78075818 78075110 ID=MS.gene057249.t1.exon6;Parent=MS.gene057249.t1
chr3.2 CDS 78075110 78075818 78075110 ID=cds.MS.gene057249.t1;Parent=MS.gene057249.t1
chr3.2 exon 78075922 78076029 78075922 ID=MS.gene057249.t1.exon7;Parent=MS.gene057249.t1
chr3.2 CDS 78075922 78076029 78075922 ID=cds.MS.gene057249.t1;Parent=MS.gene057249.t1
Gene Sequence

>MS.gene057249

ATGGGTGAGGTTGTAATGAGTGGTGAGAAAATAGTGAGAGTGGAAGAAGAAGAAGAGAAGGTGAGGGAAGAAGAAGGGAGTGGAACTGAGAGTAGAGGTGGTGGTGGTGGTGAAATGAAAGGGCTTATGAGGTGGGAGAAATTCTTGCCAAAAATGGTTTTGAGGGTGCTTTTGGTTGAAGCTGATGATTGTACAAGACAGATTATTACAGCACTTCTTAGAAAATGCAACTATAAAGGTACTATTTGTATAATTATTATTTTTTCTCTGATTTCATTGTTTTTGTTTTGTAATATTGCTGTGTGTTTAATTGGTTATTCAATTTTCTGTAAGTATTCTCAATTTTCAATTTTATGTGCTCAATTGCTTACTGTCATATGAGCATGTTATGTTGCTCTGATTATTCATATTTTTTCACATGTACAATTTTTTTTTTTGTGTGTTAAATTTGATTTAATTAATTGTAAATATTAGGTTATATTGAGGCTGAGTTGTTAATTAAGAGTATATATTTGACATGACAACAAAAATAAAAAAATAAAATAAAAGCTACTAGTAATTAGAAATTTAGAAGTCTTTGGACTGCAAATTTTGGAAAGGATCAAGGCTCAAGGGTGTACTGATAGATTTGTAGATTTTGATGATTTAATAATTACAAAAATTAAGGGGATGGAAAACAAAATTTGTGTGCACAAATTAATTGAAGACATTTTGTTTTTAATATAGTTTGATAAATTTTTAACTCATGAGAAAAAATGTAAAAAATTATGTATGTTTTTTAAAGAGAAGTGACATGGATTTTGTTAGCTGAGAATCTTAGGAATTTATGACACCTTGAAATAGAGTCTTATAGCAGTATGGAAATTACTAGCTTAAGCCATAAACTTGGTACAATTATTCAATTTATATCTGCGATAAAGATTTTATATTATTGAGTTTTATTATCTTGGTCGTATTGTAATTTGAAAAACAAAATTAGTGTACCTGAAAGTAAATCTAAGTTCTAGAACTTCAAAATAACTTTGATCTGTTTTGAGCAGTTGCTGCTGTTGCTGATGGCTTGAAGGCATGGGAAATACTCAAAGGAAGACCGCGCAGTATTGATCTGATACTTACAGAAGTCGATTTGCCAGCCATATCCGGCTACGCACTTCTCACATTAATTATGGAGCATGATATTTGCAAGAGCATCCCTGTCATAAGTATGATATTGAAGATAAATTTTCTTTCTGCTTGTCAATTTTTTTCCTTATTAATACTTAAATTAATTTTTCTGTAATTTTTTCATGCTTTTCCAGTGATGTCGTCGCAAGATTCAGTTAGCACAGTATACAAATGCATGTTGAGAGGTGCAGCTGACTATCTTGTTAAGCCGATTCGAATAAATGAACTAAGGAATTTGTGGCAGCATGTTTGGAGAAGACAATCAGTAGGACTTCTGCTCTCTCAATTTTCAGTTCGTTTTCAGTTTCTCTCTAAAATACTAAGAATTTATTAACAAAATTGGGTGAACTTATGTTTATTATTTGTTTACTCTATCAGCAGTCAGCCGCAACCGCTGGCATAAATGGCCCTCAAGATGAAAGTGATACGCAACAGAAGTTTGAAGCCACTGCTGAAAACAACGCTGCTAGTAATCGTTCCGGCGGTGATGCTGCTTGCATTCAAAGAAATAAGGACTTAATTGAGAAAGGAAGTGATGCACAGAGCTCTTGTGCTGAAGCATATCCAAGTGAAATAAAGACACAAGAACTTGACATTCATTTAGGCCAGGCAGTGATCGCACAGGACAGTCACGCCGGAGGTAAATTTCTTTCTTTCACAGCAATATCATTTTGTTCGACTCGCAAGGAAGAATTGTCAAAAATTTGAACAAACTTTTATTTTTAGGATTAAGCGTGGCTAACTGCAAAAATGGAGTGGCAAGCACAATTAACTGTAAAAATGGAGAGACAAGCGCAAATAATTGCAAGGACGGCGATGATCAAGAGCATTTTAGGAATGCTAGCATCAGTGGTGAGGTTCATGACAATCACTATGTTCAAATTTACTCTACTACTAAGGAAGCTATTGACTTGATTGGAGCATTTCGCACTGATCCGAATTGCAGTCTCAAAAATTCATCAATTGATTGCACAGGCAAGTTTGACCATTCTCCACAGTTGGATCTTTCTCTCAGAAGCTCTCATCCCAGCAACTTTGAGAAGGATCTCACCGAAGAAAGGCACACCCTTATGCATTCCAATGCTTCAGCTTTCAAACGGTAATTGCTAAATCCCTTTTTATTCTAATTTTTTCGTTTCTGTGTAATTAAAAATTGTTGTACTCTTAGTGATGAGTTTGAAGAACTTCAAAACATGATACGGATGAAGCTAGCAAGTGTATGATTTTTATGCTTGTGGTCTCTATAACTTCGCCGCTTTGTTGAATTCTGCATGCTTATCACTGAAATGGTGTTCCATTCATGCAGGTATACAAACAGGCAATTGCAAGCGTCACCTGCAGTAGTAATTAACTTCTCTGATCAACAAAGAGAACAAAAAACAAATAATGAGAACCATAACTCAGATAGTTCAATACCAAGTATGCAAAAAGAATCTGAACTTGCAACTTCACATTCTCAGCAAGGGCATTCTCTTCCGATTCCTGTAAAAGGTGTAAGGTTAAATGATCTTTGTATGGCCTATGGTTCAACACTTCCACCAGGGTTTCGTACACAATCAGGTCCACCATCAATGCCCGGTTCAGTTGTGTTCCTCGAACAAAACTTTCAAGCAGATGCATTTTATCAGTCAAATGTTAAAGAAAATAATTCAGAGCAGCTTTACGAACCTCGCGGTCTAAATGGAAACAGCACTCCAAACCAAATTGTGTATACACAGGAACACAAGTCAGAACATCCGGAGGATCAAAGACTTATCTCCCCTACAACTGATCAAAGTGTATCCAGTAGTATTTGTAATAATGGAAATGCCAGCAATTTTACCAGCATTGGTTATGGAAGTAATTGCGGAAGTAGCGGCAATGTTGAACAAGTTGCAACTTTCAGAACAGCAGCAGTTTCAGAGGGAAAGAATGAAGAACTCTCAAACAGTGGATATTCACATCGAGCTATGCTACGGGAAGCAGCTCTAAACAAGTTCCGTTTGAAACGAAAAGAGAGATGTTATGAAAAGAAGGTATATCAATATCAATATGAGCTTGTAATTATACTAGCATGATCATTGATCAATGATCATCATTGTTTTCATATTATGCTAACTTATTTATACTTTTTAACAGGTTCGATATGAGAGCAGAAAGAAACTAGCAGAGCAGCGTCCTCGAGTTAAAGGACAATTTGTTCGCCAACCGAATCCCGATTCTCTTTCTGGAGAAAAAGATTGCTAA
Protein sequence

>MS.gene057249.t1

MGEVVMSGEKIVRVEEEEEKVREEEGSGTESRGGGGGEMKGLMRWEKFLPKMVLRVLLVEADDCTRQIITALLRKCNYKVAAVADGLKAWEILKGRPRSIDLILTEVDLPAISGYALLTLIMEHDICKSIPVIMMSSQDSVSTVYKCMLRGAADYLVKPIRINELRNLWQHVWRRQSSAATAGINGPQDESDTQQKFEATAENNAASNRSGGDAACIQRNKDLIEKGSDAQSSCAEAYPSEIKTQELDIHLGQAVIAQDSHAGGLSVANCKNGVASTINCKNGETSANNCKDGDDQEHFRNASISGEVHDNHYVQIYSTTKEAIDLIGAFRTDPNCSLKNSSIDCTGKFDHSPQLDLSLRSSHPSNFEKDLTEERHTLMHSNASAFKRYTNRQLQASPAVVINFSDQQREQKTNNENHNSDSSIPSMQKESELATSHSQQGHSLPIPVKGVRLNDLCMAYGSTLPPGFRTQSGPPSMPGSVVFLEQNFQADAFYQSNVKENNSEQLYEPRGLNGNSTPNQIVYTQEHKSEHPEDQRLISPTTDQSVSSSICNNGNASNFTSIGYGSNCGSSGNVEQVATFRTAAVSEGKNEELSNSGYSHRAMLREAALNKFRLKRKERCYEKKVRYESRKKLAEQRPRVKGQFVRQPNPDSLSGEKDC