Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88937.t1 | XP_013466206.1 | 84 | 991 | 80 | 5 | 1 | 920 | 1 | 983 | 0.00E+00 | 1485.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88937.t1 | D5LXJ0 | 32.6 | 497 | 296 | 12 | 6 | 473 | 3 | 489 | 9.4e-50 | 200.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88937.t1 | A0A072VF72 | 84.0 | 991 | 80 | 5 | 1 | 920 | 1 | 983 | 0.0e+00 | 1485.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052309 | MS.gene88937 | 0.837295 | 5.66E-57 | -1.69E-46 |
| MS.gene052358 | MS.gene88937 | 0.805989 | 1.02E-49 | -1.69E-46 |
| MS.gene059033 | MS.gene88937 | 0.806654 | 7.40E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene88937 | 0.804945 | 1.69E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88937.t1 | MTR_1g026290 | 88.798 | 973 | 37 | 7 | 14 | 920 | 17 | 983 | 0.0 | 1646 |
| MS.gene88937.t1 | MTR_1g026290 | 88.332 | 977 | 38 | 7 | 14 | 920 | 17 | 987 | 0.0 | 1644 |
| MS.gene88937.t1 | MTR_1g026290 | 96.651 | 836 | 20 | 3 | 14 | 849 | 17 | 844 | 0.0 | 1591 |
| MS.gene88937.t1 | MTR_1g026290 | 96.651 | 836 | 20 | 3 | 14 | 849 | 17 | 844 | 0.0 | 1591 |
| MS.gene88937.t1 | MTR_1g026290 | 86.701 | 767 | 31 | 6 | 220 | 920 | 1 | 762 | 0.0 | 1244 |
| MS.gene88937.t1 | MTR_4g116370 | 34.615 | 520 | 279 | 16 | 10 | 493 | 19 | 513 | 2.68e-60 | 219 |
| MS.gene88937.t1 | MTR_4g116370 | 34.615 | 520 | 279 | 16 | 10 | 493 | 11 | 505 | 3.14e-60 | 219 |
| MS.gene88937.t1 | MTR_4g116370 | 33.201 | 503 | 277 | 16 | 29 | 493 | 10 | 491 | 3.25e-52 | 195 |
| MS.gene88937.t1 | MTR_4g116370 | 31.061 | 396 | 225 | 13 | 134 | 493 | 34 | 417 | 1.31e-30 | 129 |
| MS.gene88937.t1 | MTR_4g116370 | 33.569 | 283 | 160 | 9 | 234 | 493 | 58 | 335 | 4.03e-27 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88937.t1 | AT5G42220 | 46.113 | 952 | 405 | 27 | 1 | 919 | 1 | 877 | 0.0 | 659 |
| MS.gene88937.t1 | AT5G42220 | 46.113 | 952 | 405 | 27 | 1 | 919 | 1 | 877 | 0.0 | 659 |
| MS.gene88937.t1 | AT5G25270 | 34.988 | 423 | 225 | 14 | 24 | 429 | 20 | 409 | 5.74e-52 | 194 |
| MS.gene88937.t1 | AT5G11080 | 33.939 | 165 | 91 | 6 | 24 | 184 | 10 | 160 | 3.36e-13 | 72.4 |
| MS.gene88937.t1 | AT5G11080 | 33.939 | 165 | 91 | 6 | 24 | 184 | 10 | 160 | 6.80e-13 | 71.6 |
Find 283 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTTCATCTGTGGGCTTTC+TGG | 0.145540 | 1.3:-93819 | None:intergenic |
| CTCTGTTCATTTACCTGTTC+AGG | 0.186974 | 1.3:-91325 | None:intergenic |
| CTTTCTTGTACAGGAATATT+TGG | 0.188581 | 1.3:+95176 | MS.gene88937:intron |
| GTAATATGGTTTCAGGTTAT+TGG | 0.197097 | 1.3:+87238 | MS.gene88937:intron |
| TGACAAACCTGCACCATTAT+AGG | 0.224010 | 1.3:-89545 | None:intergenic |
| TAATATGGTTTCAGGTTATT+GGG | 0.238705 | 1.3:+87239 | MS.gene88937:intron |
| AAGGGCTTCTGATGATCTTT+TGG | 0.244313 | 1.3:+95012 | MS.gene88937:CDS |
| GCAGGTTGGCAGTCAGGATA+TGG | 0.262907 | 1.3:+93662 | MS.gene88937:CDS |
| GGGTGCACTTCTACTTGAAC+TGG | 0.270093 | 1.3:+89346 | MS.gene88937:CDS |
| GAAGAAGTTTGTGCGGAATT+AGG | 0.272535 | 1.3:-87310 | None:intergenic |
| CCCTGCTAGTTGATTGCATT+TGG | 0.273481 | 1.3:-91276 | None:intergenic |
| AGGTTAGTTGAAGATATATT+TGG | 0.275645 | 1.3:-88312 | None:intergenic |
| GAGCCCGGTTCTGGTGATTC+AGG | 0.283347 | 1.3:+90857 | MS.gene88937:CDS |
| TCATTTACCTGTTCAGGTCT+TGG | 0.283401 | 1.3:-91319 | None:intergenic |
| AAGCTGCTCGAAGGACGTTC+TGG | 0.292892 | 1.3:+95222 | MS.gene88937:CDS |
| CTGTCAACCTGCTGGGCAAT+CGG | 0.292962 | 1.3:-93849 | None:intergenic |
| ACTAGCAGGGACTTGTCTTC+TGG | 0.308253 | 1.3:+91289 | MS.gene88937:CDS |
| CTTGTCCCTTCCCCATTGTT+TGG | 0.310487 | 1.3:-90818 | None:intergenic |
| CAGTCCAAGAGGAGCAGTTT+TGG | 0.314139 | 1.3:-91695 | None:intergenic |
| TATTACCAACAGAGTTTCTA+AGG | 0.317262 | 1.3:-91048 | None:intergenic |
| AGGAATTGCTATGCAGCATT+TGG | 0.319967 | 1.3:+89325 | MS.gene88937:CDS |
| ACCTGCACCATTATAGGATT+TGG | 0.321015 | 1.3:-89539 | None:intergenic |
| GGGTTAGATGGGAGAGTTGC+TGG | 0.327771 | 1.3:-90914 | None:intergenic |
| GCACCAATTGTTTCAGCAAT+TGG | 0.338308 | 1.3:+90788 | MS.gene88937:CDS |
| TCTTCAACTCCTGCAACTTT+AGG | 0.346394 | 1.3:+90634 | MS.gene88937:CDS |
| CACCGTAGTGAGCCCGGTTC+TGG | 0.349753 | 1.3:+90848 | MS.gene88937:CDS |
| CTTCAAACAAGCTCCTTGTT+TGG | 0.351103 | 1.3:+90598 | MS.gene88937:CDS |
| ATGAGTCGTTGCTGATTAAC+TGG | 0.353590 | 1.3:-86607 | None:intergenic |
| GAACATGCACTCTCTCAAAA+TGG | 0.354321 | 1.3:+88209 | MS.gene88937:CDS |
| ATGAGCGGACGTGGGGCTTC+CGG | 0.360117 | 1.3:+91361 | MS.gene88937:CDS |
| ACAACTTTGTAAATCTTGAT+TGG | 0.360242 | 1.3:-91590 | None:intergenic |
| GCAGTTTATATTTCTCCATC+AGG | 0.362139 | 1.3:+89515 | MS.gene88937:CDS |
| TAATGATGAGAGAGATGTTT+TGG | 0.365444 | 1.3:+91543 | MS.gene88937:CDS |
| TTGTGACTCTAGCATATTGC+AGG | 0.375677 | 1.3:+89230 | MS.gene88937:intron |
| TTTCACACAGTGTGGTTCTT+GGG | 0.376592 | 1.3:+87031 | MS.gene88937:CDS |
| GCTGCTGGCAGCACACCATC+TGG | 0.377689 | 1.3:+90950 | MS.gene88937:CDS |
| GGAGCACTTCCAACACCAAC+GGG | 0.378459 | 1.3:-90658 | None:intergenic |
| CAGTCAGAGTCAGTACAAAT+AGG | 0.378536 | 1.3:+89305 | MS.gene88937:CDS |
| CCTCTGCCCTCCTTCCGTTC+CGG | 0.379314 | 1.3:-93697 | None:intergenic |
| CCGATGCCAGAAGGGTTAGA+TGG | 0.380811 | 1.3:-90926 | None:intergenic |
| TTAATTTGATAAATGCTGAT+AGG | 0.382229 | 1.3:+93436 | MS.gene88937:CDS |
| AGATGACAATGGAGCTGAAT+CGG | 0.385957 | 1.3:-91002 | None:intergenic |
| GATCCGTCATCAGCACCTTT+AGG | 0.387358 | 1.3:-93327 | None:intergenic |
| GGCAACCCTTGTGTATTAGA+AGG | 0.388446 | 1.3:-88357 | None:intergenic |
| ATTTCACACAGTGTGGTTCT+TGG | 0.390711 | 1.3:+87030 | MS.gene88937:CDS |
| CTAAATAGTCCTGCTTTGAA+TGG | 0.397811 | 1.3:+93522 | MS.gene88937:CDS |
| TGCCCAGCAGGTTGGCAGTC+AGG | 0.400197 | 1.3:+93656 | MS.gene88937:CDS |
| TGTTGCAACGAGAGTCTTGC+CGG | 0.402364 | 1.3:+95043 | MS.gene88937:CDS |
| CGAACCTGAATCACCAGAAC+CGG | 0.412370 | 1.3:-90861 | None:intergenic |
| ATCAGGGCCAAATCCTATAA+TGG | 0.413483 | 1.3:+89532 | MS.gene88937:CDS |
| ACTCTCCCATCTAACCCTTC+TGG | 0.413671 | 1.3:+90920 | MS.gene88937:CDS |
| GTGCTCCTCGTAGCCGTGTT+GGG | 0.414635 | 1.3:+87004 | MS.gene88937:CDS |
| GCTGGTATGACATTTCTAAC+AGG | 0.415527 | 1.3:-90896 | None:intergenic |
| ACCTTCAACCAGTGGCTGAT+AGG | 0.418486 | 1.3:+94915 | MS.gene88937:CDS |
| ACCTTTAGGAGGTTGGAGTC+TGG | 0.421199 | 1.3:-93313 | None:intergenic |
| TTGTTCAGAAACATTGGTAC+TGG | 0.424220 | 1.3:-86348 | None:intergenic |
| ATCTGAACCAAGACCTGAAC+AGG | 0.426549 | 1.3:+91312 | MS.gene88937:CDS |
| AGGAAATCAAGCTGGAAATC+AGG | 0.426719 | 1.3:+87955 | MS.gene88937:CDS |
| GGTCAGCCCGATTGCCCAGC+AGG | 0.428555 | 1.3:+93842 | MS.gene88937:CDS |
| GGAATTGCTATGCAGCATTT+GGG | 0.432404 | 1.3:+89326 | MS.gene88937:CDS |
| GCTGGAAATCAGGCACCATC+TGG | 0.433495 | 1.3:+87965 | MS.gene88937:CDS |
| TATCCTGACTGCCAACCTGC+TGG | 0.435244 | 1.3:-93659 | None:intergenic |
| TTGACTCACCTGATGGCTTA+AGG | 0.438048 | 1.3:+93577 | MS.gene88937:CDS |
| TTGTCTTTGAAGAGAGAAAC+GGG | 0.438410 | 1.3:-86562 | None:intergenic |
| CCAACGGGGCCTAAAGTTGC+AGG | 0.440187 | 1.3:-90643 | None:intergenic |
| GACAAAATTGCAACTGAAGT+AGG | 0.442503 | 1.3:+86580 | MS.gene88937:CDS |
| TTCGCAGTGTTAACATAGTT+CGG | 0.443754 | 1.3:-89370 | None:intergenic |
| GAATGGCCATACATTGCATT+TGG | 0.444494 | 1.3:+86784 | MS.gene88937:CDS |
| TCACTGCCGGACCCCGTAGC+AGG | 0.447048 | 1.3:-86836 | None:intergenic |
| CTTTGAATGGTTTGTTGGAA+GGG | 0.448136 | 1.3:+93535 | MS.gene88937:CDS |
| CCTGCCAAAACTGCTCCTCT+TGG | 0.448981 | 1.3:+91691 | MS.gene88937:CDS |
| CCTTCTGGCATCGGAGCTGC+TGG | 0.449331 | 1.3:+90935 | MS.gene88937:CDS |
| AAAGGAAACAATGTTGGATC+AGG | 0.451832 | 1.3:+86982 | MS.gene88937:intron |
| CAGACAGTGTGTTGAGGGAA+TGG | 0.453201 | 1.3:-88169 | None:intergenic |
| CGTACAGAACAGTTGCTTAG+TGG | 0.453630 | 1.3:+88411 | MS.gene88937:CDS |
| AGACAGTGTGTTGAGGGAAT+GGG | 0.453746 | 1.3:-88168 | None:intergenic |
| GTGTTGAGGGAATGGGGAAT+TGG | 0.453924 | 1.3:-88161 | None:intergenic |
| ACTGCGAATGGGACAGTCTT+CGG | 0.454831 | 1.3:+89385 | MS.gene88937:CDS |
| TCCTATCAGCCACTGGTTGA+AGG | 0.458836 | 1.3:-94916 | None:intergenic |
| TAATTCCGCACAAACTTCTT+CGG | 0.459355 | 1.3:+87312 | MS.gene88937:CDS |
| ACGGAAGGAGGGCAGAGGGG+TGG | 0.459563 | 1.3:+93702 | MS.gene88937:CDS |
| GAGCCAATTGCTGAAACAAT+TGG | 0.459970 | 1.3:-90791 | None:intergenic |
| AGCTGCTCGAAGGACGTTCT+GGG | 0.461367 | 1.3:+95223 | MS.gene88937:CDS |
| CAAAACTGCTCCTCTTGGAC+TGG | 0.461628 | 1.3:+91696 | MS.gene88937:CDS |
| TTCTTTCATCAATCAAAGTC+AGG | 0.461942 | 1.3:+93356 | MS.gene88937:CDS |
| TTTCCTTCAGTTTCGTTCCC+CGG | 0.463751 | 1.3:-87938 | None:intergenic |
| GAGGGAACTTCTGCTGATCT+AGG | 0.464850 | 1.3:+89266 | MS.gene88937:CDS |
| CTGACTACCGCTGGTGGGCA+AGG | 0.465410 | 1.3:-93469 | None:intergenic |
| CAAACAAGCTCCTTGTTTGG+CGG | 0.466746 | 1.3:+90601 | MS.gene88937:CDS |
| CAGAGTTTCTAAGGTGTGAC+TGG | 0.466854 | 1.3:-91039 | None:intergenic |
| GCAGGAGTGTGTGTCTGATT+TGG | 0.470969 | 1.3:-86818 | None:intergenic |
| GATATGGGGAATATGTTAAC+CGG | 0.471960 | 1.3:+93678 | MS.gene88937:CDS |
| GCAGAATCTGTTGTTAATGC+TGG | 0.472329 | 1.3:+89488 | MS.gene88937:CDS |
| ATGTGAATGTTTACGTTCCT+AGG | 0.472527 | 1.3:-90679 | None:intergenic |
| CATTTGAAAGGAAACAATGT+TGG | 0.473006 | 1.3:+86976 | MS.gene88937:intron |
| TTGGCTCTAGACCAAACAAT+GGG | 0.473293 | 1.3:+90807 | MS.gene88937:CDS |
| TGTCAGTCAGATTGCCCAGC+AGG | 0.474074 | 1.3:+93644 | MS.gene88937:CDS |
| TCATGTTGTTCAGATTCCTA+TGG | 0.474117 | 1.3:+88009 | MS.gene88937:CDS |
| CCATCTAACCCTTCTGGCAT+CGG | 0.474223 | 1.3:+90926 | MS.gene88937:CDS |
| CCCTTTCACTGCTAGACAGC+TGG | 0.474737 | 1.3:-94994 | None:intergenic |
| ACTTTAGGCCCCGTTGGTGT+TGG | 0.474796 | 1.3:+90649 | MS.gene88937:CDS |
| TTTCAACCAAATGCAATGTA+TGG | 0.475715 | 1.3:-86790 | None:intergenic |
| TCCTGCTTTGAATGGTTTGT+TGG | 0.475916 | 1.3:+93530 | MS.gene88937:CDS |
| AACTATGTTAACACTGCGAA+TGG | 0.476187 | 1.3:+89373 | MS.gene88937:CDS |
| TCTCGCCTTTCACTTGAAGA+TGG | 0.477787 | 1.3:-91658 | None:intergenic |
| CACACTCCTGCTACGGGGTC+CGG | 0.479486 | 1.3:+86830 | MS.gene88937:CDS |
| ACTTTGATTGATGAAAGAAC+TGG | 0.480329 | 1.3:-93352 | None:intergenic |
| AAAATATATTGCAAACACTC+GGG | 0.480549 | 1.3:+93397 | MS.gene88937:CDS |
| GCCAAATGCAATCAACTAGC+AGG | 0.482738 | 1.3:+91275 | MS.gene88937:CDS |
| GAGCTTGTTTGAAGTGGAAA+TGG | 0.485347 | 1.3:-90589 | None:intergenic |
| ACCGCGGAGATGAGCGGACG+TGG | 0.485367 | 1.3:+91352 | MS.gene88937:CDS |
| TGTCAACCTGCTGGGCAATC+GGG | 0.485522 | 1.3:-93848 | None:intergenic |
| GCATTTCTCAAGACATTACT+CGG | 0.487020 | 1.3:+87085 | MS.gene88937:CDS |
| GTTGGTTGCACTTCTGAAAG+TGG | 0.487800 | 1.3:+91394 | MS.gene88937:CDS |
| GGCTGACCATGTTCATCTGT+GGG | 0.487914 | 1.3:-93827 | None:intergenic |
| GTTCAGATTCCTATGGCTGC+GGG | 0.490634 | 1.3:+88016 | MS.gene88937:CDS |
| CCAGCAGCTCCGATGCCAGA+AGG | 0.490801 | 1.3:-90935 | None:intergenic |
| GTGGTTAGTGCTTCAAGTGT+TGG | 0.491485 | 1.3:-88378 | None:intergenic |
| ATCTCTCTCATCATTACTAC+AGG | 0.491809 | 1.3:-91536 | None:intergenic |
| CAGGTTGGCAGTCAGGATAT+GGG | 0.492293 | 1.3:+93663 | MS.gene88937:CDS |
| AATATATCTTCAACTAACCT+TGG | 0.492822 | 1.3:+88315 | MS.gene88937:CDS |
| CATCAGCACCTTTAGGAGGT+TGG | 0.493318 | 1.3:-93320 | None:intergenic |
| GCTTTGAATGGTTTGTTGGA+AGG | 0.495809 | 1.3:+93534 | MS.gene88937:CDS |
| TCAGACACACACTCCTGCTA+CGG | 0.495909 | 1.3:+86823 | MS.gene88937:CDS |
| CAGACACACACTCCTGCTAC+GGG | 0.497357 | 1.3:+86824 | MS.gene88937:CDS |
| AACATGCTCCTTAAGCCATC+AGG | 0.499359 | 1.3:-93585 | None:intergenic |
| AACAAGGAGCTTGTTTGAAG+TGG | 0.499575 | 1.3:-90595 | None:intergenic |
| CCGGAACGGAAGGAGGGCAG+AGG | 0.500044 | 1.3:+93697 | MS.gene88937:CDS |
| CCAGTTTGACCTTCAACCAG+TGG | 0.500150 | 1.3:+94907 | MS.gene88937:intron |
| ATTGTATCACAAGCCCTTGG+TGG | 0.501552 | 1.3:+93762 | MS.gene88937:CDS |
| GGTGCTCCTCGTAGCCGTGT+TGG | 0.503290 | 1.3:+87003 | MS.gene88937:CDS |
| GATGACAATGGAGCTGAATC+GGG | 0.503305 | 1.3:-91001 | None:intergenic |
| GCGAATCAATCTTCAAATGA+AGG | 0.505900 | 1.3:+86317 | MS.gene88937:CDS |
| ATTATAGGATTTGGCCCTGA+TGG | 0.508302 | 1.3:-89530 | None:intergenic |
| CGGAACGGAAGGAGGGCAGA+GGG | 0.509216 | 1.3:+93698 | MS.gene88937:CDS |
| TTTGTCTTTGAAGAGAGAAA+CGG | 0.510561 | 1.3:-86563 | None:intergenic |
| CTGGTGATTCAGGTTCGACA+CGG | 0.513653 | 1.3:+90867 | MS.gene88937:CDS |
| GATTTACAAAGTTGTTCTAG+TGG | 0.515216 | 1.3:+91598 | MS.gene88937:CDS |
| ATCTGAACAACATGAGGAAA+CGG | 0.516291 | 1.3:-88001 | None:intergenic |
| TGTGGCGGGTTTAATGTCTC+AGG | 0.517663 | 1.3:+93497 | MS.gene88937:CDS |
| TGTTCAGATTCCTATGGCTG+CGG | 0.519569 | 1.3:+88015 | MS.gene88937:CDS |
| GAACACAGACAGTGTGTTGA+GGG | 0.521105 | 1.3:-88174 | None:intergenic |
| TCCACTAAAGATGTTGAGAA+TGG | 0.521526 | 1.3:+86767 | MS.gene88937:intron |
| ATTCCTACTGCATTGAAAGA+AGG | 0.523356 | 1.3:-88052 | None:intergenic |
| GTTCTTGGGACTATTAATGT+GGG | 0.523988 | 1.3:+87045 | MS.gene88937:CDS |
| GCCAAATCCTATAATGGTGC+AGG | 0.524609 | 1.3:+89538 | MS.gene88937:CDS |
| GGTTCTTGGGACTATTAATG+TGG | 0.525047 | 1.3:+87044 | MS.gene88937:CDS |
| CAAAATATATTGCAAACACT+CGG | 0.525718 | 1.3:+93396 | MS.gene88937:CDS |
| GGAACGGAAGGAGGGCAGAG+GGG | 0.527535 | 1.3:+93699 | MS.gene88937:CDS |
| TCCAGCTGTCTAGCAGTGAA+AGG | 0.528815 | 1.3:+94993 | MS.gene88937:CDS |
| ATCTTGTTGTTCAGAAACAT+TGG | 0.528977 | 1.3:-86354 | None:intergenic |
| GGGCTGACCATGTTCATCTG+TGG | 0.529144 | 1.3:-93828 | None:intergenic |
| TCTGCTGATCTAGGTGTAAG+AGG | 0.529800 | 1.3:+89275 | MS.gene88937:CDS |
| CCAAGGGCTTGTGATACAAT+GGG | 0.531514 | 1.3:-93759 | None:intergenic |
| GCTTCCGGTGTATCCTCAGT+TGG | 0.536156 | 1.3:+91376 | MS.gene88937:CDS |
| GATGGAGAAATATAAACTGC+TGG | 0.536793 | 1.3:-89512 | None:intergenic |
| GGGGTGGTGTTGACCTCTCA+AGG | 0.536912 | 1.3:+93718 | MS.gene88937:CDS |
| TGCAGGACGCATGGAGCGAG+AGG | 0.537065 | 1.3:+89247 | MS.gene88937:CDS |
| TGGTGATTCAGGTTCGACAC+GGG | 0.537877 | 1.3:+90868 | MS.gene88937:CDS |
| CGGTAGTCAGATTGATGTGG+CGG | 0.538847 | 1.3:+93482 | MS.gene88937:CDS |
| AAAACTGCTCCTCTTGGACT+GGG | 0.539076 | 1.3:+91697 | MS.gene88937:CDS |
| TTGTATCACAAGCCCTTGGT+GGG | 0.540065 | 1.3:+93763 | MS.gene88937:CDS |
| TGACAGTGTTCATCATCTGC+GGG | 0.540994 | 1.3:-93626 | None:intergenic |
| ACCAGACTCCAACCTCCTAA+AGG | 0.542230 | 1.3:+93312 | MS.gene88937:CDS |
| ACCAAGGGCTTGTGATACAA+TGG | 0.542806 | 1.3:-93760 | None:intergenic |
| AACTCTGTTGGTAATATGCA+AGG | 0.543610 | 1.3:+91055 | MS.gene88937:CDS |
| GAAACTGAAGGAAATCAAGC+TGG | 0.543666 | 1.3:+87947 | MS.gene88937:CDS |
| TAGGAGCATTCATTGTGCCC+TGG | 0.544651 | 1.3:-87291 | None:intergenic |
| AAATCAGGCACCATCTGGAC+AGG | 0.545938 | 1.3:+87970 | MS.gene88937:CDS |
| TGCACTAGCTGTCTCACTGC+CGG | 0.547750 | 1.3:-86849 | None:intergenic |
| GTCTGTGTTCATAAATCGCA+TGG | 0.548128 | 1.3:+88187 | MS.gene88937:CDS |
| ATCCTGACTGCCAACCTGCT+GGG | 0.548407 | 1.3:-93658 | None:intergenic |
| CTAGCATATTGCAGGACGCA+TGG | 0.549101 | 1.3:+89238 | MS.gene88937:intron |
| TGACTACCGCTGGTGGGCAA+GGG | 0.550222 | 1.3:-93468 | None:intergenic |
| ACTGAACACCGTAGTGAGCC+CGG | 0.552562 | 1.3:+90842 | MS.gene88937:CDS |
| ATTGAAAGAAGGAACGGGTA+TGG | 0.552998 | 1.3:-88041 | None:intergenic |
| ATCAATCTGACTACCGCTGG+TGG | 0.553084 | 1.3:-93475 | None:intergenic |
| GGTGCACTTCTACTTGAACT+GGG | 0.554167 | 1.3:+89347 | MS.gene88937:CDS |
| AGAAAGTGGACCGCCAAACA+AGG | 0.554785 | 1.3:-90611 | None:intergenic |
| TATGAGTGGTTTAGAGCGTA+AGG | 0.555258 | 1.3:+91720 | MS.gene88937:CDS |
| TTCTGAAAGTGGAGTAGAGA+AGG | 0.555816 | 1.3:+91405 | MS.gene88937:CDS |
| ACTATGTTAACACTGCGAAT+GGG | 0.555829 | 1.3:+89374 | MS.gene88937:CDS |
| AGATATCTGTAGGTGGACTC+AGG | 0.555870 | 1.3:-94946 | None:intergenic |
| ACTGCATTGAAAGAAGGAAC+GGG | 0.556439 | 1.3:-88046 | None:intergenic |
| GGTATGGCTCCCGCAGCCAT+AGG | 0.556523 | 1.3:-88025 | None:intergenic |
| CCAGCTGTCTAGCAGTGAAA+GGG | 0.556611 | 1.3:+94994 | MS.gene88937:CDS |
| TCAATCAAAGTCAGGAAACC+AGG | 0.557847 | 1.3:+93364 | MS.gene88937:CDS |
| GAACAACATGAGGAAACGGT+TGG | 0.558266 | 1.3:-87997 | None:intergenic |
| CCCATTGTATCACAAGCCCT+TGG | 0.558380 | 1.3:+93759 | MS.gene88937:CDS |
| ATTTGAAGATTGATTCGCCA+TGG | 0.560305 | 1.3:-86312 | None:intergenic |
| GGTTCTGTAGCAGAGAATAG+TGG | 0.560598 | 1.3:-93798 | None:intergenic |
| TACTGCATTGAAAGAAGGAA+CGG | 0.562217 | 1.3:-88047 | None:intergenic |
| CGATCAATCCTATCAGCCAC+TGG | 0.564835 | 1.3:-94923 | None:intergenic |
| GTATCACAAGCCCTTGGTGG+GGG | 0.565830 | 1.3:+93765 | MS.gene88937:CDS |
| GCAGGAGTTGAAGAAGAAAG+TGG | 0.566669 | 1.3:-90625 | None:intergenic |
| CAGTTTATATTTCTCCATCA+GGG | 0.566861 | 1.3:+89516 | MS.gene88937:CDS |
| CGATGCCAGAAGGGTTAGAT+GGG | 0.567423 | 1.3:-90925 | None:intergenic |
| GACAGTGTGTTGAGGGAATG+GGG | 0.567926 | 1.3:-88167 | None:intergenic |
| ATCCTGACTGTCAACCTGCT+GGG | 0.568139 | 1.3:-93856 | None:intergenic |
| AGTCAGGAAACCAGGACAGA+TGG | 0.569397 | 1.3:+93372 | MS.gene88937:CDS |
| TTCTTGGGACTATTAATGTG+GGG | 0.569682 | 1.3:+87046 | MS.gene88937:CDS |
| AAATATATTGCAAACACTCG+GGG | 0.570038 | 1.3:+93398 | MS.gene88937:CDS |
| GGATACGATAGTGAGAAAGA+AGG | 0.570907 | 1.3:-86660 | None:intergenic |
| ATGTTAACCGGAACGGAAGG+AGG | 0.571880 | 1.3:+93690 | MS.gene88937:CDS |
| CACATCAATCTGACTACCGC+TGG | 0.573025 | 1.3:-93478 | None:intergenic |
| CCTCTTGGACTGGGTATGAG+TGG | 0.573762 | 1.3:+91706 | MS.gene88937:CDS |
| TCTTGGGACTATTAATGTGG+GGG | 0.574459 | 1.3:+87047 | MS.gene88937:CDS |
| GCTCGAAGGACGTTCTGGGA+GGG | 0.576177 | 1.3:+95227 | MS.gene88937:CDS |
| TCCAACAAACCATTCAAAGC+AGG | 0.577193 | 1.3:-93531 | None:intergenic |
| CTGACAGTGTTCATCATCTG+CGG | 0.578106 | 1.3:-93627 | None:intergenic |
| CCGTCATCAGCACCTTTAGG+AGG | 0.578916 | 1.3:-93324 | None:intergenic |
| TTGGTGTTGGAAGTGCTCCT+AGG | 0.580894 | 1.3:+90662 | MS.gene88937:CDS |
| CACCAGAACCGGGCTCACTA+CGG | 0.581466 | 1.3:-90850 | None:intergenic |
| ATGCTCCATCTTCAAGTGAA+AGG | 0.582046 | 1.3:+91653 | MS.gene88937:CDS |
| CCTGCAACTTTAGGCCCCGT+TGG | 0.585045 | 1.3:+90643 | MS.gene88937:CDS |
| ACGGGAGTTGACTCACCTGA+TGG | 0.587577 | 1.3:+93570 | MS.gene88937:CDS |
| CGCGGAGATGAGCGGACGTG+GGG | 0.588211 | 1.3:+91354 | MS.gene88937:CDS |
| TGAAACAATTGGTGCAAGAG+AGG | 0.589969 | 1.3:-90780 | None:intergenic |
| CCTTCAGTTTCGTTCCCCGG+TGG | 0.591488 | 1.3:-87935 | None:intergenic |
| CAGCGACCCTTGCCCACCAG+CGG | 0.592187 | 1.3:+93462 | MS.gene88937:CDS |
| TGTATCACAAGCCCTTGGTG+GGG | 0.593198 | 1.3:+93764 | MS.gene88937:CDS |
| CAAACAATGGGGAAGGGACA+AGG | 0.594079 | 1.3:+90819 | MS.gene88937:CDS |
| AATATGTTAACCGGAACGGA+AGG | 0.594563 | 1.3:+93687 | MS.gene88937:CDS |
| CAGCAGCTCCGATGCCAGAA+GGG | 0.595475 | 1.3:-90934 | None:intergenic |
| GGTAGTCAGATTGATGTGGC+GGG | 0.595486 | 1.3:+93483 | MS.gene88937:CDS |
| GTAGCAGAGAATAGTGGTGG+AGG | 0.597993 | 1.3:-93792 | None:intergenic |
| GTAAACATTCACATACATGC+TGG | 0.598953 | 1.3:+90688 | MS.gene88937:CDS |
| TGAACACAGACAGTGTGTTG+AGG | 0.599080 | 1.3:-88175 | None:intergenic |
| CCAAATGCAATCAACTAGCA+GGG | 0.599347 | 1.3:+91276 | MS.gene88937:CDS |
| CCTCCTAAAGGTGCTGATGA+CGG | 0.599760 | 1.3:+93324 | MS.gene88937:CDS |
| GGGGAATATGTTAACCGGAA+CGG | 0.601844 | 1.3:+93683 | MS.gene88937:CDS |
| GGTGATTCAGGTTCGACACG+GGG | 0.602931 | 1.3:+90869 | MS.gene88937:CDS |
| TCTAGACCAAACAATGGGGA+AGG | 0.604140 | 1.3:+90812 | MS.gene88937:CDS |
| AATATGGTTTCAGGTTATTG+GGG | 0.609927 | 1.3:+87240 | MS.gene88937:intron |
| TTGATTGATGAAAGAACTGG+AGG | 0.610446 | 1.3:-93349 | None:intergenic |
| CATTTCTCAAGACATTACTC+GGG | 0.611396 | 1.3:+87086 | MS.gene88937:CDS |
| AGGAGCACTTCCAACACCAA+CGG | 0.613156 | 1.3:-90659 | None:intergenic |
| AGTCAGATTGCCCAGCAGGT+TGG | 0.619153 | 1.3:+93648 | MS.gene88937:CDS |
| AGGTTGGCAGTCAGGATATG+GGG | 0.622013 | 1.3:+93664 | MS.gene88937:CDS |
| ATCGGGTGGTAGCTGTGAAG+TGG | 0.627292 | 1.3:-90984 | None:intergenic |
| TCTGTTGAAGCATCCTTGAG+AGG | 0.630215 | 1.3:-93731 | None:intergenic |
| ATAGGAATCTGAACAACATG+AGG | 0.630849 | 1.3:-88007 | None:intergenic |
| CCACTCATACCCAGTCCAAG+AGG | 0.633234 | 1.3:-91706 | None:intergenic |
| TGTTAACCGGAACGGAAGGA+GGG | 0.633672 | 1.3:+93691 | MS.gene88937:CDS |
| TTCAGAAGTGCAACCAACTG+AGG | 0.633745 | 1.3:-91389 | None:intergenic |
| GGTTGGAATGCCTGTCCAGA+TGG | 0.633876 | 1.3:-87980 | None:intergenic |
| TGGGCAAATTTCACACAGTG+TGG | 0.634556 | 1.3:+87023 | MS.gene88937:CDS |
| GAACCTGAATCACCAGAACC+GGG | 0.636088 | 1.3:-90860 | None:intergenic |
| ATTCTGCTCGTTGATCTCCA+AGG | 0.637073 | 1.3:-88332 | None:intergenic |
| CCGCGGAGATGAGCGGACGT+GGG | 0.638934 | 1.3:+91353 | MS.gene88937:CDS |
| CCACCGGGGAACGAAACTGA+AGG | 0.639658 | 1.3:+87935 | MS.gene88937:CDS |
| TGCTCGAAGGACGTTCTGGG+AGG | 0.640302 | 1.3:+95226 | MS.gene88937:CDS |
| GAATTGCCTTCTAATACACA+AGG | 0.641086 | 1.3:+88351 | MS.gene88937:CDS |
| CTGGTGGGCAAGGGTCGCTG+AGG | 0.641101 | 1.3:-93459 | None:intergenic |
| ACAGCACGGAAGATATCTGT+AGG | 0.645362 | 1.3:-94956 | None:intergenic |
| TCAGCTAAAACAGATGACAA+TGG | 0.645638 | 1.3:-91013 | None:intergenic |
| TGTGTGAAATTTGCCCAACA+CGG | 0.647094 | 1.3:-87017 | None:intergenic |
| TCAATCTGACTACCGCTGGT+GGG | 0.647138 | 1.3:-93474 | None:intergenic |
| GATGTAAGTAAGCTGCTCGA+AGG | 0.650206 | 1.3:+95213 | MS.gene88937:CDS |
| AGAGATACCGCGGAGATGAG+CGG | 0.652439 | 1.3:+91346 | MS.gene88937:CDS |
| ATTGGCTCTAGACCAAACAA+TGG | 0.655176 | 1.3:+90806 | MS.gene88937:CDS |
| TCTGTAGCAGAGAATAGTGG+TGG | 0.656147 | 1.3:-93795 | None:intergenic |
| CAGCGGTAGTCAGATTGATG+TGG | 0.657434 | 1.3:+93479 | MS.gene88937:CDS |
| AGAAAGCCCACAGATGAACA+TGG | 0.659366 | 1.3:+93821 | MS.gene88937:CDS |
| GCAGGACGCATGGAGCGAGA+GGG | 0.662033 | 1.3:+89248 | MS.gene88937:CDS |
| CTAGACCAAACAATGGGGAA+GGG | 0.662121 | 1.3:+90813 | MS.gene88937:CDS |
| TCACACCTTAGAAACTCTGT+TGG | 0.665440 | 1.3:+91043 | MS.gene88937:CDS |
| CCCACGTCCGCTCATCTCCG+CGG | 0.669954 | 1.3:-91353 | None:intergenic |
| GACAATGGAGCTGAATCGGG+TGG | 0.672692 | 1.3:-90998 | None:intergenic |
| GCAACCAACTGAGGATACAC+CGG | 0.678404 | 1.3:-91380 | None:intergenic |
| ACTGACCGAAGAAGTTTGTG+CGG | 0.679232 | 1.3:-87317 | None:intergenic |
| GCTAGTGCAAATAGTAACCG+AGG | 0.687450 | 1.3:+86863 | MS.gene88937:CDS |
| GCACGGAAGATATCTGTAGG+TGG | 0.700840 | 1.3:-94953 | None:intergenic |
| TCACAACCAGACCCAAACTG+AGG | 0.703188 | 1.3:+91504 | MS.gene88937:intron |
| AATATATTGCAAACACTCGG+GGG | 0.705829 | 1.3:+93399 | MS.gene88937:CDS |
| ATTTGCCCAACACGGCTACG+AGG | 0.706337 | 1.3:-87009 | None:intergenic |
| TTCTGTACGATGCAATACAG+TGG | 0.709351 | 1.3:-88397 | None:intergenic |
| GTGGAGATACTGAATCCAGA+TGG | 0.710982 | 1.3:-90965 | None:intergenic |
| AAATGAACAGAGAGATACCG+CGG | 0.715935 | 1.3:+91336 | MS.gene88937:CDS |
| AATTGCCTTCTAATACACAA+GGG | 0.735513 | 1.3:+88352 | MS.gene88937:CDS |
| TGGCTCTAGACCAAACAATG+GGG | 0.735668 | 1.3:+90808 | MS.gene88937:CDS |
| AGACACACACTCCTGCTACG+GGG | 0.754610 | 1.3:+86825 | MS.gene88937:CDS |
| GAGCACTTCCAACACCAACG+GGG | 0.778341 | 1.3:-90657 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 86314 | 95260 | 86314 | ID=MS.gene88937 |
| chr1.3 | mRNA | 86314 | 95260 | 86314 | ID=MS.gene88937.t1;Parent=MS.gene88937 |
| chr1.3 | exon | 86314 | 86445 | 86314 | ID=MS.gene88937.t1.exon1;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 86314 | 86445 | 86314 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 86559 | 86676 | 86559 | ID=MS.gene88937.t1.exon2;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 86559 | 86676 | 86559 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 86777 | 86884 | 86777 | ID=MS.gene88937.t1.exon3;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 86777 | 86884 | 86777 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 86986 | 87107 | 86986 | ID=MS.gene88937.t1.exon4;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 86986 | 87107 | 86986 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 87253 | 87333 | 87253 | ID=MS.gene88937.t1.exon5;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 87253 | 87333 | 87253 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 87932 | 88066 | 87932 | ID=MS.gene88937.t1.exon6;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 87932 | 88066 | 87932 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 88161 | 88230 | 88161 | ID=MS.gene88937.t1.exon7;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 88161 | 88230 | 88161 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 88304 | 88455 | 88304 | ID=MS.gene88937.t1.exon8;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 88304 | 88455 | 88304 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 89242 | 89406 | 89242 | ID=MS.gene88937.t1.exon9;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 89242 | 89406 | 89242 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 89488 | 89559 | 89488 | ID=MS.gene88937.t1.exon10;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 89488 | 89559 | 89488 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 90589 | 90709 | 90589 | ID=MS.gene88937.t1.exon11;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 90589 | 90709 | 90589 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 90777 | 91097 | 90777 | ID=MS.gene88937.t1.exon12;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 90777 | 91097 | 90777 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 91275 | 91426 | 91275 | ID=MS.gene88937.t1.exon13;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 91275 | 91426 | 91275 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 91514 | 91741 | 91514 | ID=MS.gene88937.t1.exon14;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 91514 | 91741 | 91514 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 93306 | 93863 | 93306 | ID=MS.gene88937.t1.exon15;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 93306 | 93863 | 93306 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 94911 | 95066 | 94911 | ID=MS.gene88937.t1.exon16;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 94911 | 95066 | 94911 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| chr1.3 | exon | 95189 | 95260 | 95189 | ID=MS.gene88937.t1.exon17;Parent=MS.gene88937.t1 |
| chr1.3 | CDS | 95189 | 95260 | 95189 | ID=cds.MS.gene88937.t1;Parent=MS.gene88937.t1 |
| Gene Sequence |
| Protein sequence |
