AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89051

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89051.t1 MTR_2g103370 99.044 837 8 0 1 837 1 837 0.0 1707
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89051.t1 AT2G41620 76.134 838 196 4 1 835 1 837 0.0 1313
MS.gene89051.t1 AT3G57350 73.302 839 217 6 1 835 1 836 0.0 1251

Find 223 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTCTGGCAATGAGATTT+TGG 0.159071 2.4:+2096465 MS.gene89051:CDS
CGCTCAGTTCCCTCCTCTTC+AGG 0.194338 2.4:+2089191 MS.gene89051:CDS
CAGATTCGGTTGAGGGATTA+TGG 0.218780 2.4:+2093601 MS.gene89051:intron
ATGATGGTTCAAATTTATTT+AGG 0.234703 2.4:-2095277 None:intergenic
AAAGGATGTCATTGATTATT+GGG 0.266183 2.4:+2093250 MS.gene89051:CDS
GGAATCATGTTGATGCATTA+AGG 0.269660 2.4:+2097199 MS.gene89051:CDS
AAAAGGATGTCATTGATTAT+TGG 0.273592 2.4:+2093249 MS.gene89051:CDS
ATCCCGTGGATTCCCTTCTT+TGG 0.277216 2.4:+2097407 MS.gene89051:CDS
CAAGGGTGCATACGAAAGTT+TGG 0.280694 2.4:+2093000 MS.gene89051:CDS
TGATTATATGATGAAAGTTT+TGG 0.290773 2.4:+2091290 MS.gene89051:CDS
TTCACATCAATTTGCTCCTT+TGG 0.293661 2.4:+2094854 MS.gene89051:CDS
CCCTGGTCAGATTGTGTCTA+TGG 0.300095 2.4:+2092255 MS.gene89051:CDS
CTTTCTGAAGGCATCGGTAC+TGG 0.302430 2.4:+2095461 MS.gene89051:CDS
AATAATCAATGACATCCTTT+TGG 0.303162 2.4:-2093247 None:intergenic
CAGAAGTTTCCACAATTATC+AGG 0.308981 2.4:+2095513 MS.gene89051:CDS
TCCACAATTATCAGGCAATA+TGG 0.312023 2.4:+2095521 MS.gene89051:CDS
TAGACCCATCGCAGATAAAA+AGG 0.313748 2.4:+2092330 MS.gene89051:CDS
AATGCTTAAAAGCAAGATAT+AGG 0.316195 2.4:-2095336 None:intergenic
GGTTATCCTTCTGAGCTTCC+TGG 0.316241 2.4:-2091254 None:intergenic
TAATAATTTCACCTGACATC+TGG 0.338858 2.4:-2096505 None:intergenic
GCAGCAGTAGCCACCTCTTC+TGG 0.339368 2.4:-2094987 None:intergenic
CTGCTGTACGCCATCATATC+AGG 0.341394 2.4:+2095083 MS.gene89051:CDS
CGCTTCAGCTCTTACAGTCT+TGG 0.344037 2.4:-2089463 None:intergenic
AAAGAATCCGCGCCTTCCTT+CGG 0.347541 2.4:+2093483 MS.gene89051:CDS
AAAGATATGGCACCTTGTTC+AGG 0.349423 2.4:+2093060 MS.gene89051:CDS
TCGACGCTTGTTGCCTCAAC+AGG 0.349996 2.4:-2090699 None:intergenic
TGCCGGAGAAGAAGGAGAAT+TGG 0.353697 2.4:+2095748 MS.gene89051:CDS
TCTAACTGAATTATTGTTAA+GGG 0.357730 2.4:+2095691 MS.gene89051:CDS
GTTGAGGGATTATGGGGTTC+TGG 0.358855 2.4:+2093609 MS.gene89051:CDS
TTTAAGCATTCAATTGCTTC+CGG 0.360646 2.4:+2095349 MS.gene89051:CDS
TCTACTCCACAAGTCTCTTC+TGG 0.369570 2.4:+2092280 MS.gene89051:CDS
CGTGGATTCCCTTCTTTGGT+TGG 0.378457 2.4:+2097411 MS.gene89051:CDS
GGCTGTATTATACTTGTCTA+AGG 0.380243 2.4:+2095370 MS.gene89051:CDS
TCGTTCTCAGGTTCATGAAA+TGG 0.382869 2.4:+2091215 MS.gene89051:intron
AAGAATCCGCGCCTTCCTTC+GGG 0.385464 2.4:+2093484 MS.gene89051:CDS
CAATGTTGTTTAGAGGAACT+TGG 0.385531 2.4:+2089407 MS.gene89051:intron
ACTGCTGGCCTTATCCATTC+TGG 0.387759 2.4:+2096451 MS.gene89051:CDS
TTCTAACTGAATTATTGTTA+AGG 0.393622 2.4:+2095690 MS.gene89051:CDS
TTTGAAGTCATTTGAGTTGA+AGG 0.396716 2.4:+2090166 MS.gene89051:CDS
AGATTCGGTTGAGGGATTAT+GGG 0.398506 2.4:+2093602 MS.gene89051:intron
TTCAGAGAGGCACTTGTTGA+TGG 0.398841 2.4:-2096383 None:intergenic
CAGTAACGATGCGAAAGATA+TGG 0.400785 2.4:+2093047 MS.gene89051:CDS
TATCTAATGTAGGCTTCTCT+TGG 0.401085 2.4:+2093440 MS.gene89051:intron
GCTGCTGCTGCAGTCGGCGG+TGG 0.402127 2.4:+2095599 MS.gene89051:CDS
GCATGCCAGTGTCAGGAGGC+TGG 0.405039 2.4:+2095821 MS.gene89051:CDS
GCTTCTCTTGGTGGTGGTGT+TGG 0.405273 2.4:+2093452 MS.gene89051:CDS
CTGAATAGAGCAAGGCAGTC+TGG 0.408430 2.4:+2092382 MS.gene89051:CDS
AAGGGTGCATACGAAAGTTT+GGG 0.416231 2.4:+2093001 MS.gene89051:CDS
GAAGGTTGCTCTGACTTGTC+TGG 0.416937 2.4:+2097332 MS.gene89051:CDS
ATGCTTAAAAGCAAGATATA+GGG 0.417403 2.4:-2095335 None:intergenic
CTCACAGAGTGGATTAATAC+TGG 0.419658 2.4:+2094954 MS.gene89051:CDS
ATAGCTTGCCGCAACTTTCA+AGG 0.420161 2.4:+2092982 MS.gene89051:intron
TTTCTTGTGGTTCAAATTGT+CGG 0.427428 2.4:+2095169 MS.gene89051:CDS
AATTCACAAATTGTCAAGAC+TGG 0.427511 2.4:+2097176 MS.gene89051:CDS
GGGCAAGGCGCCACCTTGAG+TGG 0.430038 2.4:+2093271 MS.gene89051:CDS
TGCTGCGCAGATTCGGTTGA+GGG 0.430889 2.4:+2093594 MS.gene89051:intron
CTAATGTAGGCTTCTCTTGG+TGG 0.431600 2.4:+2093443 MS.gene89051:intron
GCAAGAGGTGCCGGAGAAGA+AGG 0.439378 2.4:+2095740 MS.gene89051:CDS
TTGTATACCAGACCTCTTGA+AGG 0.447490 2.4:+2097314 MS.gene89051:CDS
AACAGCAATATCAGGTCCTC+GGG 0.447540 2.4:-2097256 None:intergenic
AGCGGCTTGTTCGAGAAGCT+TGG 0.447832 2.4:-2089163 None:intergenic
AACACATCAACAGCAATATC+AGG 0.449707 2.4:-2097264 None:intergenic
TCCCAATTCTCCTTCTTCTC+CGG 0.453235 2.4:-2095750 None:intergenic
CATCGGTACTGGGCAAAAGT+TGG 0.453262 2.4:+2095472 MS.gene89051:CDS
GTGAAGTAGATCGGTCCAAC+TGG 0.454319 2.4:-2089133 None:intergenic
CATGCCAGTGTCAGGAGGCT+GGG 0.455198 2.4:+2095822 MS.gene89051:CDS
TGTTGATACCACATGGCAAC+AGG 0.460280 2.4:+2093666 MS.gene89051:CDS
GCCGGAGAAGAAGGAGAATT+GGG 0.461170 2.4:+2095749 MS.gene89051:CDS
TTACTTGGTGCAAGAGGTGC+CGG 0.461950 2.4:+2095731 MS.gene89051:CDS
TTAGCAATCATGTTTGTCCT+TGG 0.468677 2.4:-2092214 None:intergenic
GAATCATGTTGATGCATTAA+GGG 0.468904 2.4:+2097200 MS.gene89051:CDS
AGCAATGTGGGTGCCCGCCC+TGG 0.473304 2.4:+2092238 MS.gene89051:CDS
AAGCTCAGAAGGATAACCTT+AGG 0.474420 2.4:+2091259 MS.gene89051:CDS
CCAAAATCTCATTGCCAGAA+TGG 0.474737 2.4:-2096465 None:intergenic
TGCAAATCATCCAAACTGTA+TGG 0.475966 2.4:-2095248 None:intergenic
GTTCAAATTGTCGGCTGTGC+GGG 0.476027 2.4:+2095178 MS.gene89051:CDS
TGAAGCGGCATGCCAGTGTC+AGG 0.479438 2.4:+2095814 MS.gene89051:CDS
GATCGGCTAGCACAATTGAT+AGG 0.481113 2.4:-2095430 None:intergenic
TTGCTGCGCAGATTCGGTTG+AGG 0.481656 2.4:+2093593 MS.gene89051:intron
GGATATAGACCAACCAAAGA+AGG 0.481700 2.4:-2097420 None:intergenic
ATCGGTACTGGGCAAAAGTT+GGG 0.482123 2.4:+2095473 MS.gene89051:CDS
TGGTTCCTTCTCAATGGCGC+TGG 0.484367 2.4:+2096357 MS.gene89051:CDS
TTGGGTGTTGATGCTGGTGG+TGG 0.485994 2.4:+2093019 MS.gene89051:CDS
TCGTCCATTGTTCTAAGTGA+TGG 0.486274 2.4:+2095218 MS.gene89051:CDS
AATGTTGAGAACTGGTGATC+GGG 0.486654 2.4:+2095028 MS.gene89051:CDS
TCTCATTGCCAGAATGGATA+AGG 0.486894 2.4:-2096459 None:intergenic
CTAGCTGCTGCTGCAGTCGG+CGG 0.493608 2.4:+2095596 MS.gene89051:CDS
TGGTCTGATTCCATACAGTT+TGG 0.493704 2.4:+2095238 MS.gene89051:CDS
GGCAATGGTCTCTGCCGTCC+AGG 0.496524 2.4:+2091236 MS.gene89051:CDS
CCTTGCGACGTGTTTCCAAA+AGG 0.497996 2.4:+2093232 MS.gene89051:CDS
TCAATTGTGCTAGCCGATCA+TGG 0.498941 2.4:+2095434 MS.gene89051:CDS
TGTTGCCATGTGGTATCAAC+AGG 0.498947 2.4:-2093664 None:intergenic
AACAATGGACGAAGATCCAC+TGG 0.500001 2.4:-2095207 None:intergenic
GGTGGACAGTTGTCGTGGAC+TGG 0.502670 2.4:+2095617 MS.gene89051:CDS
GAAAGTTTGGGTGTTGATGC+TGG 0.502944 2.4:+2093013 MS.gene89051:CDS
AGAGCAACCTTCAAGAGGTC+TGG 0.503916 2.4:-2097321 None:intergenic
GGCAAGGCGCCACCTTGAGT+GGG 0.505759 2.4:+2093272 MS.gene89051:CDS
GAGAGTTGGTTCCTTCTCAA+TGG 0.507390 2.4:+2096351 MS.gene89051:CDS
GATGGTGAGAGCCGGACTGC+TGG 0.508649 2.4:+2096436 MS.gene89051:CDS
GAATAACACATTACTTGTGC+GGG 0.509007 2.4:-2093333 None:intergenic
TGTGAAAGAATGTTGAGAAC+TGG 0.510947 2.4:+2095020 MS.gene89051:CDS
TGCGATGGGTCTACTAGCCA+TGG 0.511840 2.4:-2092320 None:intergenic
TGGGCAAAAGTTGGGAGTGA+TGG 0.514092 2.4:+2095481 MS.gene89051:CDS
TTTCTGAAGGCATCGGTACT+GGG 0.514619 2.4:+2095462 MS.gene89051:CDS
AATAATTTCACCTGACATCT+GGG 0.517279 2.4:-2096504 None:intergenic
TCTAAGGAAGCAGGAGATGA+AGG 0.518714 2.4:+2095386 MS.gene89051:CDS
CAGTTGTCGTGGACTGGTAG+AGG 0.522609 2.4:+2095623 MS.gene89051:CDS
AATGGATAAGGCCAGCAGTC+CGG 0.524111 2.4:-2096447 None:intergenic
GCACTAGCTGCTGCTGCAGT+CGG 0.524782 2.4:+2095593 MS.gene89051:CDS
CAACAGCAATATCAGGTCCT+CGG 0.524800 2.4:-2097257 None:intergenic
GGCTAGCACAATTGATAGGT+GGG 0.527583 2.4:-2095426 None:intergenic
GGATCTATGTATTTGCGTCT+TGG 0.529918 2.4:+2095542 MS.gene89051:CDS
ACTGAATTATTGTTAAGGGA+TGG 0.530872 2.4:+2095695 MS.gene89051:CDS
ATTTCCTTCAGAGTTTAAGC+AGG 0.532379 2.4:+2092179 MS.gene89051:CDS
TTATACTTGTCTAAGGAAGC+AGG 0.533407 2.4:+2095377 MS.gene89051:CDS
GGCTTGTTCGAGAAGCTTGG+AGG 0.534973 2.4:-2089160 None:intergenic
GCGTCTTGGTGATCTTCAGA+TGG 0.537770 2.4:+2095556 MS.gene89051:CDS
AAATCCTGCTTAAACTCTGA+AGG 0.537989 2.4:-2092183 None:intergenic
ATACTTACTACCCAGATGTC+AGG 0.538443 2.4:+2096494 MS.gene89051:CDS
ATAGAAATTCAGAAGAGAGT+TGG 0.538979 2.4:+2096337 MS.gene89051:CDS
ATTCACAAATTGTCAAGACT+GGG 0.541007 2.4:+2097177 MS.gene89051:CDS
TGCCGTCCAGGAAGCTCAGA+AGG 0.542467 2.4:+2091248 MS.gene89051:CDS
GGATAAATCTTCGTTCGCCA+TGG 0.543632 2.4:-2089112 None:intergenic
ACAAGTCTCTTCTGGTAGCA+TGG 0.544655 2.4:+2092288 MS.gene89051:CDS
AGACACAATCTGACCAGGGC+GGG 0.545465 2.4:-2092251 None:intergenic
AGGGAACTGAGCGGAAGGAG+CGG 0.547144 2.4:-2089181 None:intergenic
TCTTCGAGAACCTGATATGA+TGG 0.547163 2.4:-2095093 None:intergenic
TTCAGAAAGGACGCCATGAT+CGG 0.547176 2.4:-2095447 None:intergenic
GAATGTTGAGAACTGGTGAT+CGG 0.548567 2.4:+2095027 MS.gene89051:CDS
AGAGGTCTGGTATACAAGCT+TGG 0.551386 2.4:-2097308 None:intergenic
GAGTTGGTTGATCTCTAAGC+AGG 0.552770 2.4:-2095127 None:intergenic
TATAATCATTGAAACTCCTA+AGG 0.553720 2.4:-2091275 None:intergenic
ATGTAGGCTTCTCTTGGTGG+TGG 0.555114 2.4:+2093446 MS.gene89051:intron
GAAACAGATGCTGATGGATG+AGG 0.555847 2.4:+2093203 MS.gene89051:intron
CGTCGAAGAGTATCTGCAGC+AGG 0.556391 2.4:+2090716 MS.gene89051:CDS
TAAATGCAGAGCAGCTTGCA+AGG 0.557101 2.4:+2090141 MS.gene89051:CDS
TAGCTTGCCGCAACTTTCAA+GGG 0.559302 2.4:+2092983 MS.gene89051:intron
AGAGTACCTAGTTGCAGCAA+TGG 0.560531 2.4:-2089496 None:intergenic
TCCATATTGCCTGATAATTG+TGG 0.560589 2.4:-2095522 None:intergenic
GTGGATTAATACTGGAGGAA+TGG 0.561929 2.4:+2094962 MS.gene89051:CDS
GTATGCACCCTTGAAAGTTG+CGG 0.563942 2.4:-2092990 None:intergenic
GGTATCCAGCGCCATTGAGA+AGG 0.565340 2.4:-2096362 None:intergenic
GAATTATTGTTAAGGGATGG+AGG 0.568335 2.4:+2095698 MS.gene89051:CDS
TGTCATTGATTATTGGGGCA+AGG 0.568514 2.4:+2093256 MS.gene89051:CDS
GCAAGATATAGGGATATACC+AGG 0.570594 2.4:-2095325 None:intergenic
CGGCTAGCACAATTGATAGG+TGG 0.571111 2.4:-2095427 None:intergenic
ACTTCGTGCTTTGAGAGCTA+AGG 0.571432 2.4:+2097380 MS.gene89051:CDS
GCCATAGACACAATCTGACC+AGG 0.571806 2.4:-2092256 None:intergenic
TCTCACCATCTAACCTTCCA+CGG 0.572182 2.4:-2096423 None:intergenic
ATTTCTCATGTCCCCACTCA+AGG 0.575810 2.4:-2093284 None:intergenic
GAACTGGTGATCGGGTAGGC+CGG 0.576829 2.4:+2095036 MS.gene89051:CDS
TTAGACAAGTATAATACAGC+CGG 0.577630 2.4:-2095368 None:intergenic
GGTATACAAGCTTGGACATG+AGG 0.577743 2.4:-2097300 None:intergenic
TGGAGGAATGGTGCCAGAAG+AGG 0.578745 2.4:+2094974 MS.gene89051:CDS
GATTCGGTTGAGGGATTATG+GGG 0.578836 2.4:+2093603 MS.gene89051:intron
TCGGCGGTGGACAGTTGTCG+TGG 0.580039 2.4:+2095612 MS.gene89051:CDS
ATCCTTCTGAGCTTCCTGGA+CGG 0.581007 2.4:-2091250 None:intergenic
GGCGTCCTTTCTGAAGGCAT+CGG 0.581437 2.4:+2095455 MS.gene89051:CDS
GACAATGTGACAGACTCTGA+TGG 0.582532 2.4:+2097354 MS.gene89051:CDS
TAGCATGGAGATTGTTCCCA+TGG 0.582635 2.4:+2092303 MS.gene89051:CDS
ATACAGGCTTCTTGCTCGTG+AGG 0.583757 2.4:+2090115 MS.gene89051:intron
CAACCAAAGAAGGGAATCCA+CGG 0.583960 2.4:-2097410 None:intergenic
GATATAGACCAACCAAAGAA+GGG 0.585209 2.4:-2097419 None:intergenic
TGAATAACACATTACTTGTG+CGG 0.585399 2.4:-2093334 None:intergenic
ACATCCCAGCCTCCTGACAC+TGG 0.585632 2.4:-2095826 None:intergenic
GATCATGGCGTCCTTTCTGA+AGG 0.591595 2.4:+2095449 MS.gene89051:CDS
TACAGGCTTCTTGCTCGTGA+GGG 0.592156 2.4:+2090116 MS.gene89051:intron
CGAACGAAGATTTATCCAGT+TGG 0.592391 2.4:+2089118 MS.gene89051:CDS
GAAGGTTAGATGGTGAGAGC+CGG 0.594461 2.4:+2096428 MS.gene89051:CDS
CACAGTCCATTGCTGCAACT+AGG 0.595342 2.4:+2089490 MS.gene89051:CDS
AAGGATGTCATTGATTATTG+GGG 0.595466 2.4:+2093251 MS.gene89051:CDS
ATGCTACCAGAAGAGACTTG+TGG 0.595882 2.4:-2092286 None:intergenic
GCAAGGCGCCACCTTGAGTG+GGG 0.595896 2.4:+2093273 MS.gene89051:CDS
CTAATTGAAACAGATGCTGA+TGG 0.595941 2.4:+2093197 MS.gene89051:intron
ATTGAGAGAACCTGAAGAGG+AGG 0.596014 2.4:-2089201 None:intergenic
CAGTACCGATGCCTTCAGAA+AGG 0.598482 2.4:-2095460 None:intergenic
GAGGTAATGTTGATCAACAA+AGG 0.600325 2.4:+2095642 MS.gene89051:CDS
GGGACATGAGAAATACATCA+TGG 0.600464 2.4:+2093293 MS.gene89051:CDS
AGGAATGGTGCCAGAAGAGG+TGG 0.600952 2.4:+2094977 MS.gene89051:CDS
AAGTCAGAGCAACCTTCAAG+AGG 0.601145 2.4:-2097326 None:intergenic
CAACAAAAGCAACTAAAGCA+TGG 0.605798 2.4:-2097441 None:intergenic
GTTGAGACGAACCCGAAGGA+AGG 0.608990 2.4:-2093495 None:intergenic
GGAGGAAGAGTGAAGTAGAT+CGG 0.609858 2.4:-2089142 None:intergenic
AGCGGCATGCCAGTGTCAGG+AGG 0.613053 2.4:+2095817 MS.gene89051:CDS
AGAGGAGGGAACTGAGCGGA+AGG 0.613962 2.4:-2089186 None:intergenic
AGAGCTAAGGTAATATCCCG+TGG 0.614814 2.4:+2097393 MS.gene89051:CDS
TCAGGTTCATGAAATGGCAA+TGG 0.615202 2.4:+2091221 MS.gene89051:intron
CAGACCATCACTTAGAACAA+TGG 0.617186 2.4:-2095222 None:intergenic
GCGATGGGTCTACTAGCCAT+GGG 0.617383 2.4:-2092319 None:intergenic
CAACAAGTACAACCTGAACA+AGG 0.624193 2.4:-2093072 None:intergenic
TGCCATGTGGTATCAACAGG+TGG 0.627027 2.4:-2093661 None:intergenic
ATGATTGCTAATAGCAATGT+GGG 0.627930 2.4:+2092226 MS.gene89051:CDS
GCGATTGAGAGAACCTGAAG+AGG 0.631149 2.4:-2089204 None:intergenic
TTGAGAACTGGTGATCGGGT+AGG 0.632782 2.4:+2095032 MS.gene89051:CDS
TTGTAGCGGATCCAAATGCA+AGG 0.634980 2.4:+2095777 MS.gene89051:CDS
CAAGATATAGGGATATACCA+GGG 0.636735 2.4:-2095324 None:intergenic
TCAGGTCCTCGGGGATCTAG+AGG 0.636836 2.4:-2097246 None:intergenic
GCTGTGCGGGACTGCCCCAG+TGG 0.639313 2.4:+2095191 MS.gene89051:CDS
GCACGATAGAGGATTTCTTG+TGG 0.640475 2.4:+2095156 MS.gene89051:CDS
GCAGGATTTCAACATTACCA+AGG 0.642066 2.4:+2092197 MS.gene89051:CDS
TTGAGAGAACCTGAAGAGGA+GGG 0.643698 2.4:-2089200 None:intergenic
GGTTCAAATTGTCGGCTGTG+CGG 0.644450 2.4:+2095177 MS.gene89051:CDS
GTTGCAGCAATGGACTGTGA+CGG 0.646464 2.4:-2089486 None:intergenic
GACGAACCCGAAGGAAGGCG+CGG 0.646810 2.4:-2093490 None:intergenic
AAATGCAGAGCAGCTTGCAA+GGG 0.647635 2.4:+2090142 MS.gene89051:CDS
AACCAAAGAAGGGAATCCAC+GGG 0.647934 2.4:-2097409 None:intergenic
TCATGTTGATGCATTAAGGG+AGG 0.649430 2.4:+2097203 MS.gene89051:CDS
CATGATTGCTAATAGCAATG+TGG 0.654831 2.4:+2092225 MS.gene89051:CDS
AGCCACCTGTTGATACCACA+TGG 0.668750 2.4:+2093659 MS.gene89051:CDS
GTTTCTTACAGCTCACAGAG+TGG 0.670484 2.4:+2094943 MS.gene89051:intron
TCTCATGTCCCCACTCAAGG+TGG 0.676167 2.4:-2093281 None:intergenic
TTCACAAATTGTCAAGACTG+GGG 0.679733 2.4:+2097178 MS.gene89051:CDS
AAGATATAGGGATATACCAG+GGG 0.683071 2.4:-2095323 None:intergenic
ACAATGGACGAAGATCCACT+GGG 0.689644 2.4:-2095206 None:intergenic
ACAGAGTGGATTAATACTGG+AGG 0.690461 2.4:+2094957 MS.gene89051:CDS
CCATAGACACAATCTGACCA+GGG 0.701596 2.4:-2092255 None:intergenic
GAGAACAAATAGCTTCAGAG+AGG 0.705513 2.4:-2096396 None:intergenic
CTGAAGAGGAGGGAACTGAG+CGG 0.706436 2.4:-2089190 None:intergenic
AGTCAAGACCTGTTGCCATG+TGG 0.718128 2.4:-2093674 None:intergenic
AGTTTGGGTGTTGATGCTGG+TGG 0.734098 2.4:+2093016 MS.gene89051:CDS
TAGACACAATCTGACCAGGG+CGG 0.737233 2.4:-2092252 None:intergenic
ACAGCAATATCAGGTCCTCG+GGG 0.757630 2.4:-2097255 None:intergenic
CAATGGACGAAGATCCACTG+GGG 0.814652 2.4:-2095205 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.4 gene 2089114 2097464 2089114 ID=MS.gene89051
chr2.4 mRNA 2089114 2097464 2089114 ID=MS.gene89051.t1;Parent=MS.gene89051
chr2.4 exon 2089114 2089212 2089114 ID=MS.gene89051.t1.exon1;Parent=MS.gene89051.t1
chr2.4 CDS 2089114 2089212 2089114 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2089420 2089511 2089420 ID=MS.gene89051.t1.exon2;Parent=MS.gene89051.t1
chr2.4 CDS 2089420 2089511 2089420 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2090121 2090187 2090121 ID=MS.gene89051.t1.exon3;Parent=MS.gene89051.t1
chr2.4 CDS 2090121 2090187 2090121 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2090678 2090737 2090678 ID=MS.gene89051.t1.exon4;Parent=MS.gene89051.t1
chr2.4 CDS 2090678 2090737 2090678 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2091225 2091314 2091225 ID=MS.gene89051.t1.exon5;Parent=MS.gene89051.t1
chr2.4 CDS 2091225 2091314 2091225 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2092154 2092417 2092154 ID=MS.gene89051.t1.exon6;Parent=MS.gene89051.t1
chr2.4 CDS 2092154 2092417 2092154 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2092986 2093081 2092986 ID=MS.gene89051.t1.exon7;Parent=MS.gene89051.t1
chr2.4 CDS 2092986 2093081 2092986 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2093210 2093341 2093210 ID=MS.gene89051.t1.exon8;Parent=MS.gene89051.t1
chr2.4 CDS 2093210 2093341 2093210 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2093452 2093505 2093452 ID=MS.gene89051.t1.exon9;Parent=MS.gene89051.t1
chr2.4 CDS 2093452 2093505 2093452 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2093604 2093687 2093604 ID=MS.gene89051.t1.exon10;Parent=MS.gene89051.t1
chr2.4 CDS 2093604 2093687 2093604 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2094789 2094875 2094789 ID=MS.gene89051.t1.exon11;Parent=MS.gene89051.t1
chr2.4 CDS 2094789 2094875 2094789 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2094954 2095856 2094954 ID=MS.gene89051.t1.exon12;Parent=MS.gene89051.t1
chr2.4 CDS 2094954 2095856 2094954 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2096334 2096515 2096334 ID=MS.gene89051.t1.exon13;Parent=MS.gene89051.t1
chr2.4 CDS 2096334 2096515 2096334 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
chr2.4 exon 2097110 2097464 2097110 ID=MS.gene89051.t1.exon14;Parent=MS.gene89051.t1
chr2.4 CDS 2097110 2097464 2097110 ID=cds.MS.gene89051.t1;Parent=MS.gene89051.t1
Gene Sequence

>MS.gene89051

ATGGCGAACGAAGATTTATCCAGTTGGACCGATCTACTTCACTCTTCCTCCAAGCTTCTCGAACAAGCCGCTCCTTCCGCTCAGTTCCCTCCTCTTCAGGTTCTCTCAATCGCACCATTTTTCACTCTTCTTTTTTTAGGTTAAGTTTATATGCATTTTGATTTTCAACTATGCTTAGCTTCAATTCGATTGAATAATTGGATAATTATTACTTTCTGTTAGTTAGAATTGTTTTTGACTGAAAATTGAAATAATTATTGATTTTTACTATGATTTTGGGGATTTATTTTTTTCAATGTTGTTTAGAGGAACTTGGATCAACTTGAAGCTTTATCGAAGAAGCTTAAATCCAAGACTGTAAGAGCTGAAGCGCCGTCACAGTCCATTGCTGCAACTAGGTACTCTTGTTAATGCGAAATTTATATTCTGTTCATAAGTTGTTCTATTATGTTTTGGTAAGTTTCTAGTTTAGTTGGATTGCTTTTAGTGCATGTTTGGTTTTGCCGTGACAAAAATTGATTTTGTAAAATTGATTTTACTTAAAAGTGAGTTGAAGGATTGTAAAAGGATTTATGTTTGGTTGGATACATTTAGGTAAAAGTGAGTTGAAAAATAAATTTCAGTTAAACAACACGTTTAGAATTAAAGCTAAAAATCCTGGATTTAAGATAGAATCAATTATGGAGGCAAAATCAATTTTACTCAAGAGCAACCAAACATGTCAAAATCAATTTTACACTTTTAGAATCAATTCTGGCTGATCTGAACGTAGAACCAATTTCTTTGATTATGATACATTATTATTCTTTCAAATGCTTTTTTTGCTTTCTCAGTAGGGGCATTGCTTTTGTTTTACATATTTCTAATAGGGCATGTGGAGCCATCATACATGTCCTAATAGACTGCCTTCCATCATTTTCTTAAAGCTAAAGTTACTTGAATTCACGACAGCATATAGGCAATTAGTGTAATAATTTCTGTTATTTATTTTGCACTCACATATACAGGCTTCTTGCTCGTGAGGGTATAAATGCAGAGCAGCTTGCAAGGGATTTGAAGTCATTTGAGTTGAAGGTACCACTTAACATATATTTTGCAATCATTTTTACATTTAACATCTTATTGTTGTTGCTTTGCGTACCATGTGCGTCTTGTGCGGGTATTGCTTAATAAAATTTGCCGTTTCAAAAAAAAAAAAAACATCTTTACCTTTTCTTGGATTGTACTATTCTATTTATTTGCCCTTACACTATAATTTATGCACAACAAATTAGTTTTCATTATTTTTGAGCTGCCATACTTAAAGTTGCACAAACTTCCTTGTTTTCATGATATAACCTATTTGGATAGAATTCCTTGTATAAACTTTCCTGATGTAACCATACTTTAAGTTGTATAGACATCCTTGTTTTCCTGATATAACCTAATTGGATGTAAACGTAATTTTGTTGGAAGTTGACGTCAAATTACAAGCTTAGTTGCTTTTGTTGGGATTTAAATTCTTCAACTCATATATTTTTTTGCTAATGCTGTGCCCTTTGCCTGTTTTTGTTGTTTTGTAAAGACAACTTTTGAGGATGTTTTTCCTGTTGAGGCAACAAGCGTCGAAGAGTATCTGCAGCAGGTAATTTCTACTTTCAAATTGCTTGCTATCCATCTTGGATTTAGTTATCACTTTAAAATCATCATTAGACATCAGTTTGATTCAATCTGGATAGTTTTTATGCGTGTTAGATATCATGTACTCTAAGTCTACCTTTTTGAGTTTGCTTTCATTTTTCTCCAGCAAAAATGTTCACAAAAATCAATGAAAACTATAATGTCGGAGTTCTGAATAGGATGTTTGATTTATTCAATAAATCACTAGTCAAAGTTAAAGAGCTGTCCTCTGAACTATCTTAAAAAAAAAAAGAGCTGTCTTTTGTTGATGTGCGTGGTATTGTAGCATGTTCCCCCTTATTTTTTATGAGCATAGCAAAATTAGTTCAGTGTTTGTATGTGAAGTTATGTACCTTGCTATGGGAAACCAATTAAGTCTCCGTACTCTTCACTTGACTTGGTCCTTTCTGAATAGAATTGTGACAATATTGAATACTCTTTTTCGTTCTCAGGTTCATGAAATGGCAATGGTCTCTGCCGTCCAGGAAGCTCAGAAGGATAACCTTAGGAGTTTCAATGATTATATGATGAAAGTTTTGGAGGTTAGTGTAATCTTGCCTTTCATGTCATGGCACACCATGATTTTTTCTGTATTCTTTGTGTAATTATTGGTTATTACTGGAGAAATTTTTGTAATGTGTTTTGCTAAGCTATTTATAAAATAAGAGTTATTGGGGTAAGATTTGGTAAACCATTAATTTGGATACTCAATGTGTCCTGTCTTTTTATTGCTTTGCTGAAAATAAGATATCTTATGGATGTGCCTATTTTATTTTCTTTATTTATTTGATTGTAGTCAAAAAGCCTTAAACTTTCAAAAGGAAATATCAGTCCTTATATTTTTGTGTGGATATGGGTACAAGATTATGACAAAGGAATTGAAGGGATTTATTGAACTATAATAAGGTTGGTTGTTTTTGCATACTTTTTGATGAGGATATATCCTATTTCGGTGAACCTTGTCTGTAATCCTTGTTTCCTCTGTGTATGAAAATTTCTCACATTCTTTTAGAAAGTCCAATATTAAAAATGAATAGAAAACAAAATATGTAAAGCGTAATTTTTGTTTAATTTATACAACTCGATAGTTTTCCATAATTTTGACTTTGGCAATGAGGTTTACTGACCTACTTAAACTTCTCACCAGTCAAATCATCCCATTTGGCTGATTGATTGTTTTTCTATAAAATATGCTTAGGTTAACATGTGGAACTTCAATATGAAATGAAGTAGCATAGTATATATATCATATTTGCAATTTTATAATTGGGATGAGTATTTTATTGCATTGTTATATTGGTCATAAAAATATGTTTATTTGTTCTTGATATTATGTGGCTCAACCTTCTTACTGCTCAGTAGCATTTAATGTTTTTGACAGGAGGATTGGCAAAAAGAAAAGCGTGATTTCCTTCAGAGTTTAAGCAGGATTTCAACATTACCAAGGACAAACATGATTGCTAATAGCAATGTGGGTGCCCGCCCTGGTCAGATTGTGTCTATGGCTTCTACTCCACAAGTCTCTTCTGGTAGCATGGAGATTGTTCCCATGGCTAGTAGACCCATCGCAGATAAAAAGGCATCTGTCTATGCTGAAGTAGTTAAAAATCTGAATAGAGCAAGGCAGTCTGGATTGCCATTTAAAGTAAGCCCCCTACTTTCAGAGCTCTATATTAGATCAAAAACCTAGTAGATGGTGTAGCCTGTTCATGACTTCAGTGATCGTGTTAGGAACCCAAATTCAAGAGAAATAGAAATTGTATTATTGATTGGAATAAGGAGAACTATTACAGAGAATGATCCCTATACTCCCAGAGGAAAAGCTCCTCAGAGAAGAGACATTTCTCTCTCTCTGCCCAAATTACAAGATTCTAACTAAATAATGGTGAATACTCCTCTCAATTGCTATCCCTATCCTTTATATAACTAACATTTCTCTAACAAACTAGCCCCCTCACCCAATGTAACTAACTCTCCTAGTAAGCACATTAGTTGGGCTTGACCCCAAACTCAGTACCCAACAGATCAACATTGTGATTTGCATGAAGCTAATGTATAAACTTTTAATTCAATACTGTTACAACATCATTTTGAACATAAGCAATGCTTTATTGTTTTTGAGATCCTTTTCCAAAAGTTCTTACTAGTATTCACCTTTTTGTATTTATATGTATGTACGCTAATTTTCATAATAATTTTTCTATGCTTGATAGCTTGCCGCAACTTTCAAGGGTGCATACGAAAGTTTGGGTGTTGATGCTGGTGGTGGAAAATCAGTAACGATGCGAAAGATATGGCACCTTGTTCAGGTTGTACTTGTTGTTGTATACTGTTAACAGGCAAAACAGAGATAAACTTTTGCCTGCAACTTTATTGGTTCTACCTGTTATGAAGTAAACATTTTTATTTATTTTCTAGACTTGGCTAATTGAAACAGATGCTGATGGATGAGGACTCAACCTTGCGACGTGTTTCCAAAAGGATGTCATTGATTATTGGGGCAAGGCGCCACCTTGAGTGGGGACATGAGAAATACATCATGGATACTATACATAATCATCCCGCACAAGTAATGTGTTATTCAGAATTTCAAGTGGTTTATTCATATGAGAGCATGCATTGATTTTTATTTGTTGTATCAACACTTAATTTCTGACATCTTTTTTTTATCTAATGTAGGCTTCTCTTGGTGGTGGTGTTGGAAACTTGCAAAGAATCCGCGCCTTCCTTCGGGTTCGTCTCAACTTGAAGAATATGCATCTTGTTAAACACTTTACCATGTATTTAATTTTTTTTTTCTTCAAAAAATCCCTGATTTTATTGCTGCGCAGATTCGGTTGAGGGATTATGGGGTTCTGGATTTTGATGCTGGTGATGCTCGGAGACAGCCACCTGTTGATACCACATGGCAACAGGTCTTGACTACTTTATCTTACTATAAAATTATTAGGGACGAGACTTGAAATTGAATCCGAAGGGTGCATACAAATTACTCGCCATGAGTACGATTTTTAGTCATCAAATGGATTTGGGTCTAGGCATGGGTAATACTAATACATATAAAAAATTGTGGGCATTACCATGGGGCATGGGGGCCATATGTTTTATTATATTTTATCCAAACGATATGCTTCATATATTTTATAACTAGCCGTAACAGTAATACCCATGCTTTGTAAGTTTGTATATGTTACTTTTGAATGGAGTAAATTCCGTTTTTTAATTATATGTTAATCATCTTATAAAGAATTTAGAGTGAAAGAAAAAAAGGTTCCGGGCCTCCAAGAGTATGTGAAAAAAGCTTTAATGCAAAAAAGTATCTATAAAAACTACGGGTATAAGTATCTAAACCTACATCTTACCTGCACAATATGCTCATCATATTTTATAACAACACGTTATACCCTTCCTTTGTATGGGTTACTTATAAATGAAACAAGAATTCTGTTTAAAAAAGGGCAACCCGGTGCACTAAAGCTCCCGCATACGCAGGGTCCGGGAAGGGAAGTCGTGGTACTGTTGGGATATGTAGACAGATTTATAGAACTCTATAGATTAGGATAGGATTTCAAATATCTCTCTGGATATTATTTAATGAAGTTAGATTTTACACTTGTCAATGCTATTTTTGTTGACTTATCATATACTCAAATAAATTTACCAGTATCTTAAAAGAAAAAGATACATCAATGGAAACAAATCCCATAACCACAACATGAGTTTGTTTGCGTTCATATCCGAGTATATAGGCATCTATTAGGTACGGTTAAGGGCCTCCAAGTTGCTACCCGCATGAGTTTGGGTTTGTGGCAATTTCTTAAATGTGGATATGGATATTCTAGTTTTTTACATAGACTCCACGCTTTGCTATCCCTACAATTTAGTTACACTTCATTGTTTCTTTTCTTTTCATGTTTTTTCTATGCTTTAACAGAGTTCAGTCCTATATCTAATTTTTTTGTTGCTTAAAATCAATATTAATGATGAGCTAAGCTTGTACTTTCTTTTTTTAACAGATATATTTTTGTCTGAGGTCTGGTTATTACGATGAAGCAAGAAATGTTGCTCTATCTTCGCGTGCTTCACATCAATTTGCTCCTTTGGTACATATACATAGTGTGATCACTTCTTGTTATGTGCTTATTTATAGTGCACTTTTAATAATTTACTGTTTCTTACAGCTCACAGAGTGGATTAATACTGGAGGAATGGTGCCAGAAGAGGTGGCTACTGCTGCATCAGAAGAATGTGAAAGAATGTTGAGAACTGGTGATCGGGTAGGCCGGACTGCATATGACAAGAAAAAATTACTGCTGTACGCCATCATATCAGGTTCTCGAAGACATATTGATCGCCTGCTTAGAGATCAACCAACTCTTTTTAGCACGATAGAGGATTTCTTGTGGTTCAAATTGTCGGCTGTGCGGGACTGCCCCAGTGGATCTTCGTCCATTGTTCTAAGTGATGGTCTGATTCCATACAGTTTGGATGATTTGCAAAGTTACCTAAATAAATTTGAACCATCATACTATACAAAAAATGGAAAAGATCCCCTGGTATATCCCTATATCTTGCTTTTAAGCATTCAATTGCTTCCGGCTGTATTATACTTGTCTAAGGAAGCAGGAGATGAAGGATATAACATTGATGCTGCCCACCTATCAATTGTGCTAGCCGATCATGGCGTCCTTTCTGAAGGCATCGGTACTGGGCAAAAGTTGGGAGTGATGGATGCTTATGCAGAAGTTTCCACAATTATCAGGCAATATGGATCTATGTATTTGCGTCTTGGTGATCTTCAGATGGCACTAGAATATTATGCACTAGCTGCTGCTGCAGTCGGCGGTGGACAGTTGTCGTGGACTGGTAGAGGTAATGTTGATCAACAAAGGCAGAGAAACTTGATGCTGAAGCAGCTTCTAACTGAATTATTGTTAAGGGATGGAGGTATATATCTTTTACTTGGTGCAAGAGGTGCCGGAGAAGAAGGAGAATTGGGACGTTTTGTAGCGGATCCAAATGCAAGGCAACAATTTTTAATTGAAGCGGCATGCCAGTGTCAGGAGGCTGGGATGTATGACAAAGTAATTTTTTTCTCATGCTACTCTTTTATTTTAGAGGAACAATAGGGAAAGTAAGAAAACCTTCAACTGCTTTTACTGAATTACAACATTACCTAGCTCACAATCATTGTTCTTCATGCCCTTATTGCGGCACTTAGATATGGAAATTACTCCACTCAGTTTTTAGACCTTTGTTTTGGCCTCTTAGACATCCTTTTTCTGGTGAAGCTAAAGATATTTTTAACACCATCTAGGAATTTTGCTAACTCTTGTTTATTTTCAATTTCTGATACCTTTTTGATTACATTTCATTATTTCTCACATTTGTGTCTCTCATACTGTGTCTCTACTCTCTAGATTTTTCCTACCTAGGTTATTTAAACTTATTTTCTCCTAATTTTTGTTACCTCCGTGTTCCCTTATTCATCACCATTTGTGAGTAAGTTTTTTCATCCAACCTTTATGTTCCTTTTGCTGATGCGGAATTTTTTGGTTCAGTCTATAGAAATTCAGAAGAGAGTTGGTTCCTTCTCAATGGCGCTGGATACCATCAACAAGTGCCTCTCTGAAGCTATTTGTTCTCTTTTCCGTGGAAGGTTAGATGGTGAGAGCCGGACTGCTGGCCTTATCCATTCTGGCAATGAGATTTTGGAGACATATACTTACTACCCAGATGTCAGGTGAAATTATTACCTTTTATTTCCTTGCTCACATATTTCTCTGCTTGGACTTTGGGATTGTGATATTCTTAAACTTTGCATGATATTTCAAAATTGAACCTTTTCAAGTTTAGAACTTATTCTGTGGTGATTTGACACAGATTATGCTTTGGTTGTTATTTTCATCTTTTCCCTTATCTTTTTTCATGGAATGATCTTTGAGGTCAAACCCTGCTTGTAACTTGGAAGTCCATTTTCGCCATATCCTTACTTCACATGGCTATTGGTGGTAATTTACATGCAAAAACAGGTGGCACAAAGATGAATGACGGGTGATATGGGATATACCATTCACAGGATTACGCCATCTTTTTGCTGATACTACGGTTGTAGTCTTTGTTAGGTGTTGGTTTTGTTTTGTTTTTTCTGCTGTACCAAAAAAAGCTATCAGCCTAATCAATTTGTAACTTTCTAAATAAAATTTGTCAGTTTTGGCTCTTCAAACTAAATATCTATGGAAGTTTCTTTCAAGTTTCAACTATGAAGAATTTGTTGTTTATCAATCTATAGCACAATCCTTAAGAAAAAAAATGATCAATTGAATTCTTTTTGCAGTCATCAAGAGAGAGAGCAAGTTTTTGAGCAGCAAACTGTATTGAGACAACTTGAGTCAATATTATCAATTCACAAATTGTCAAGACTGGGGAATCATGTTGATGCATTAAGGGAGGTTGCTAAACTTCCATTTTTGCCTCTAGATCCCCGAGGACCTGATATTGCTGTTGATGTGTTTGAAAATTTATCTCCTCATGTCCAAGCTTGTATACCAGACCTCTTGAAGGTTGCTCTGACTTGTCTGGACAATGTGACAGACTCTGATGGATCACTTCGTGCTTTGAGAGCTAAGGTAATATCCCGTGGATTCCCTTCTTTGGTTGGTCTATATCCATGCTTTAGTTGCTTTTGTTGA
Protein sequence

>MS.gene89051.t1

MANEDLSSWTDLLHSSSKLLEQAAPSAQFPPLQRNLDQLEALSKKLKSKTVRAEAPSQSIAATRLLAREGINAEQLARDLKSFELKTTFEDVFPVEATSVEEYLQQVHEMAMVSAVQEAQKDNLRSFNDYMMKVLEEDWQKEKRDFLQSLSRISTLPRTNMIANSNVGARPGQIVSMASTPQVSSGSMEIVPMASRPIADKKASVYAEVVKNLNRARQSGLPFKLAATFKGAYESLGVDAGGGKSVTMRKIWHLVQMLMDEDSTLRRVSKRMSLIIGARRHLEWGHEKYIMDTIHNHPAQASLGGGVGNLQRIRAFLRIRLRDYGVLDFDAGDARRQPPVDTTWQQIYFCLRSGYYDEARNVALSSRASHQFAPLLTEWINTGGMVPEEVATAASEECERMLRTGDRVGRTAYDKKKLLLYAIISGSRRHIDRLLRDQPTLFSTIEDFLWFKLSAVRDCPSGSSSIVLSDGLIPYSLDDLQSYLNKFEPSYYTKNGKDPLVYPYILLLSIQLLPAVLYLSKEAGDEGYNIDAAHLSIVLADHGVLSEGIGTGQKLGVMDAYAEVSTIIRQYGSMYLRLGDLQMALEYYALAAAAVGGGQLSWTGRGNVDQQRQRNLMLKQLLTELLLRDGGIYLLLGARGAGEEGELGRFVADPNARQQFLIEAACQCQEAGMYDKSIEIQKRVGSFSMALDTINKCLSEAICSLFRGRLDGESRTAGLIHSGNEILETYTYYPDVSHQEREQVFEQQTVLRQLESILSIHKLSRLGNHVDALREVAKLPFLPLDPRGPDIAVDVFENLSPHVQACIPDLLKVALTCLDNVTDSDGSLRALRAKVISRGFPSLVGLYPCFSCFC