Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89168.t1 | XP_003627334.2 | 100 | 107 | 0 | 0 | 1 | 107 | 22 | 128 | 2.60E-51 | 211.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89168.t1 | Q8LFL8 | 87.9 | 107 | 13 | 0 | 1 | 107 | 22 | 128 | 3.3e-47 | 188.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene89168.t1 | G7L7M4 | 100.0 | 107 | 0 | 0 | 1 | 107 | 22 | 128 | 1.9e-51 | 211.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051088 | MS.gene89168 | 0.938193 | 1.01E-98 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGATGCTTCAGAACTAAA+AGG | 0.302591 | 8.1:-78089470 | MS.gene89168:CDS |
| GGGGAAAATGTTGTTCTAAT+TGG | 0.311566 | 8.1:-78091076 | MS.gene89168:CDS |
| AAGAATGGAGTTCCTTGACT+TGG | 0.481713 | 8.1:-78089435 | MS.gene89168:CDS |
| GCAGCTAACGCTGTTCTTGA+GGG | 0.491345 | 8.1:-78091166 | MS.gene89168:intron |
| GTGTTCCTACAGAAGAAATC+AGG | 0.501365 | 8.1:-78090925 | MS.gene89168:CDS |
| TTCTTGAGGGTGCCTGTGAG+AGG | 0.503890 | 8.1:-78091153 | MS.gene89168:CDS |
| GTAGGAACACGCGTCATATG+TGG | 0.510896 | 8.1:+78090938 | None:intergenic |
| TGCAGCTAACGCTGTTCTTG+AGG | 0.511473 | 8.1:-78091167 | MS.gene89168:intron |
| GATATTTCAGGCACAGAAAG+TGG | 0.522168 | 8.1:-78089495 | MS.gene89168:intron |
| CTATATTGTGACATCCCTCT+AGG | 0.524354 | 8.1:-78091115 | MS.gene89168:CDS |
| ACGAGATGGTAGAAAGCTCA+TGG | 0.524959 | 8.1:-78091290 | MS.gene89168:CDS |
| GGGATGCTTCAGAACTAAAA+GGG | 0.534576 | 8.1:-78089469 | MS.gene89168:CDS |
| TAGGAACACGCGTCATATGT+GGG | 0.543008 | 8.1:+78090939 | None:intergenic |
| TCAGGCACAGAAAGTGGAAA+GGG | 0.543526 | 8.1:-78089489 | MS.gene89168:intron |
| GCCTGTGAGAGGGTAATTGT+TGG | 0.562739 | 8.1:-78091142 | MS.gene89168:CDS |
| CTAGGTCTTTATGTAATCCG+TGG | 0.570170 | 8.1:-78091097 | MS.gene89168:CDS |
| TGTTGTTCTAATTGGTGAGA+TGG | 0.571580 | 8.1:-78091068 | MS.gene89168:intron |
| CTTCTTATATTGCTACGAGA+TGG | 0.573728 | 8.1:-78091304 | MS.gene89168:CDS |
| CGAGATGGTAGAAAGCTCAT+GGG | 0.590247 | 8.1:-78091289 | MS.gene89168:CDS |
| TAGGTCTTTATGTAATCCGT+GGG | 0.590765 | 8.1:-78091096 | MS.gene89168:CDS |
| TTCAGGCACAGAAAGTGGAA+AGG | 0.592068 | 8.1:-78089490 | MS.gene89168:intron |
| TCTTGAGGGTGCCTGTGAGA+GGG | 0.598880 | 8.1:-78091152 | MS.gene89168:CDS |
| ACCCTCCTGATTTCTTCTGT+AGG | 0.607048 | 8.1:+78090920 | None:intergenic |
| ACCAACAATTACCCTCTCAC+AGG | 0.608128 | 8.1:+78091141 | None:intergenic |
| AGGAACACGCGTCATATGTG+GGG | 0.634207 | 8.1:+78090940 | None:intergenic |
| TTCCTACAGAAGAAATCAGG+AGG | 0.637433 | 8.1:-78090922 | MS.gene89168:CDS |
| TTAATCACTAGTCCAAGTCA+AGG | 0.651795 | 8.1:+78089423 | None:intergenic |
| GGATTACATAAAGACCTAGA+GGG | 0.677541 | 8.1:+78091101 | None:intergenic |
| GGAACACGCGTCATATGTGG+GGG | 0.684221 | 8.1:+78090941 | None:intergenic |
| CGGATTACATAAAGACCTAG+AGG | 0.700189 | 8.1:+78091100 | None:intergenic |
| TCCTACAGAAGAAATCAGGA+GGG | 0.714873 | 8.1:-78090921 | MS.gene89168:intron |
| GAGATGGTAGAAAGCTCATG+GGG | 0.721011 | 8.1:-78091288 | MS.gene89168:CDS |
| AGGTCTTTATGTAATCCGTG+GGG | 0.758020 | 8.1:-78091095 | MS.gene89168:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATTATACGACAATAAT+AGG | + | chr8.1:78089960-78089979 | None:intergenic | 15.0% |
| !!! | ATCATAAAGAAGTTTAATTT+AGG | + | chr8.1:78089852-78089871 | None:intergenic | 15.0% |
| !!! | TAAGATTTTATTTTTTAAGC+AGG | + | chr8.1:78090984-78091003 | None:intergenic | 15.0% |
| !!! | TTGATATTTTTGTTATGATT+TGG | - | chr8.1:78091126-78091145 | MS.gene89168:CDS | 15.0% |
| !! | AAATAAAGTGGATAAACTAA+TGG | - | chr8.1:78090325-78090344 | MS.gene89168:intron | 20.0% |
| !! | ATCATAACAAAAATATCAAC+TGG | + | chr8.1:78091125-78091144 | None:intergenic | 20.0% |
| !! | GTTAAATATTCTATATTGAG+TGG | - | chr8.1:78091215-78091234 | MS.gene89168:intron | 20.0% |
| !! | TTGAATTAATCCTAAATTTC+AGG | - | chr8.1:78090955-78090974 | MS.gene89168:CDS | 20.0% |
| !!! | AAAAGTACAATAATTGTGTT+TGG | + | chr8.1:78090190-78090209 | None:intergenic | 20.0% |
| !!! | AGAAATAGTTTTAAATGCAA+AGG | + | chr8.1:78089716-78089735 | None:intergenic | 20.0% |
| !!! | GAAATAGTTTTAAATGCAAA+GGG | + | chr8.1:78089715-78089734 | None:intergenic | 20.0% |
| !!! | TGAAATTTAGGATTAATTCA+AGG | + | chr8.1:78090956-78090975 | None:intergenic | 20.0% |
| !!! | TTCAAGGATATATCAAATTT+TGG | + | chr8.1:78090940-78090959 | None:intergenic | 20.0% |
| ! | GAGAGAATATATAGCTAATT+AGG | - | chr8.1:78090508-78090527 | MS.gene89168:intron | 25.0% |
| ! | TAACTAATTAGCTACAACTA+AGG | + | chr8.1:78090621-78090640 | None:intergenic | 25.0% |
| ! | TAGTCAGTTAGAAATTAGAT+TGG | - | chr8.1:78090079-78090098 | MS.gene89168:intron | 25.0% |
| ! | TTATGTATTACCACTGTAAA+TGG | + | chr8.1:78090870-78090889 | None:intergenic | 25.0% |
| ! | TTGGTCAATTTATACATTGA+AGG | - | chr8.1:78090807-78090826 | MS.gene89168:intron | 25.0% |
| !! | AAATTAGATTGGTCATGTTT+GGG | - | chr8.1:78090090-78090109 | MS.gene89168:intron | 25.0% |
| !! | TGCATACTTCATAATCATTT+TGG | - | chr8.1:78090348-78090367 | MS.gene89168:intron | 25.0% |
| ACATCCTGAAAATTGAAAGA+CGG | + | chr8.1:78089762-78089781 | None:intergenic | 30.0% | |
| ACTTGAGAATATAGCTTAGT+TGG | - | chr8.1:78090547-78090566 | MS.gene89168:intron | 30.0% | |
| CATTGTTTGTAAAGAGAAGT+AGG | + | chr8.1:78089993-78090012 | None:intergenic | 30.0% | |
| CTACTTCTCTTTACAAACAA+TGG | - | chr8.1:78089991-78090010 | MS.gene89168:intron | 30.0% | |
| CTTACCAATCATATGTACAA+TGG | + | chr8.1:78091031-78091050 | None:intergenic | 30.0% | |
| GATATGTTAAAGAGGTCATT+TGG | + | chr8.1:78090898-78090917 | None:intergenic | 30.0% | |
| GCAATGTAGATATGTTAAAG+AGG | + | chr8.1:78090906-78090925 | None:intergenic | 30.0% | |
| TAAGCTAGTACGACTTATTA+GGG | - | chr8.1:78090427-78090446 | MS.gene89168:intron | 30.0% | |
| TACAATGGACAAGTAGTTTA+TGG | + | chr8.1:78091016-78091035 | None:intergenic | 30.0% | |
| TACTATATTGAAATGCTTCG+TGG | - | chr8.1:78090775-78090794 | MS.gene89168:intron | 30.0% | |
| TATAACTCAACCATTTACAG+TGG | - | chr8.1:78090857-78090876 | MS.gene89168:intron | 30.0% | |
| TTAAGCTAGTACGACTTATT+AGG | - | chr8.1:78090426-78090445 | MS.gene89168:intron | 30.0% | |
| TTGTCCATTGTACATATGAT+TGG | - | chr8.1:78091024-78091043 | MS.gene89168:intron | 30.0% | |
| ! | AGATGTTTTCTTTGTGTAGT+TGG | - | chr8.1:78090464-78090483 | MS.gene89168:intron | 30.0% |
| ! | GAAATTAGATTGGTCATGTT+TGG | - | chr8.1:78090089-78090108 | MS.gene89168:intron | 30.0% |
| AAGCAGGATTCCTGAAATTT+AGG | + | chr8.1:78090968-78090987 | None:intergenic | 35.0% | |
| AATTAGCTACAACTAAGGAC+AGG | + | chr8.1:78090616-78090635 | None:intergenic | 35.0% | |
| ACATTGTATTGCCTTAAGTG+AGG | + | chr8.1:78089909-78089928 | None:intergenic | 35.0% | |
| AGGGACTATGAGAAAAAGAA+TGG | - | chr8.1:78091289-78091308 | MS.gene89168:CDS | 35.0% | |
| CAGAGTTAGAGTTACAGAAT+TGG | - | chr8.1:78090119-78090138 | MS.gene89168:intron | 35.0% | |
| CATTGTATTGCCTTAAGTGA+GGG | + | chr8.1:78089908-78089927 | None:intergenic | 35.0% | |
| CTACATGAAACAGATTCGTA+GGG | - | chr8.1:78090048-78090067 | MS.gene89168:intron | 35.0% | |
| CTAGCTTAATCGCTAGAATT+TGG | + | chr8.1:78090415-78090434 | None:intergenic | 35.0% | |
| CTTCTTATATTGCTACGAGA+TGG | - | chr8.1:78089435-78089454 | MS.gene89168:CDS | 35.0% | |
| GAGTGGAAATGTGATATTTC+AGG | - | chr8.1:78091232-78091251 | MS.gene89168:intron | 35.0% | |
| GGATTACATAAAGACCTAGA+GGG | + | chr8.1:78089641-78089660 | None:intergenic | 35.0% | |
| GGGGAAAATGTTGTTCTAAT+TGG | - | chr8.1:78089663-78089682 | MS.gene89168:intron | 35.0% | |
| GTAAAGATTTGACATGCGTT+AGG | - | chr8.1:78089519-78089538 | MS.gene89168:intron | 35.0% | |
| GTTAGCTGCATGTTATTGAT+AGG | + | chr8.1:78089562-78089581 | None:intergenic | 35.0% | |
| TAGGAGGTTATCTCATAACT+CGG | - | chr8.1:78090148-78090167 | MS.gene89168:intron | 35.0% | |
| TAGGTCTTTATGTAATCCGT+GGG | - | chr8.1:78089643-78089662 | MS.gene89168:intron | 35.0% | |
| TCTACATGAAACAGATTCGT+AGG | - | chr8.1:78090047-78090066 | MS.gene89168:intron | 35.0% | |
| TGTATGACTGCATGTCAAAA+CGG | + | chr8.1:78091169-78091188 | None:intergenic | 35.0% | |
| TGTTGTTCTAATTGGTGAGA+TGG | - | chr8.1:78089671-78089690 | MS.gene89168:intron | 35.0% | |
| ! | ACAACCGTCTTTCAATTTTC+AGG | - | chr8.1:78089755-78089774 | MS.gene89168:intron | 35.0% |
| ! | ATATGATTGGTAAGCTCTAG+TGG | - | chr8.1:78091037-78091056 | MS.gene89168:intron | 35.0% |
| ! | CTTCGCTCTTTTGATCAATT+TGG | - | chr8.1:78089477-78089496 | MS.gene89168:CDS | 35.0% |
| ! | GATATAGTGTGGGTTACTTT+AGG | + | chr8.1:78090233-78090252 | None:intergenic | 35.0% |
| ! | GTTACAGAATTGGCTTAGAT+AGG | - | chr8.1:78090129-78090148 | MS.gene89168:intron | 35.0% |
| ! | TGCTTCGTGGTTATGATTTT+TGG | - | chr8.1:78090788-78090807 | MS.gene89168:intron | 35.0% |
| ! | TTAGAACAACATTTTCCCCA+CGG | + | chr8.1:78089662-78089681 | None:intergenic | 35.0% |
| !! | GTCTTTCAATTTTCAGGATG+TGG | - | chr8.1:78089761-78089780 | MS.gene89168:intron | 35.0% |
| AAGAATGGAGTTCCTTGACT+TGG | - | chr8.1:78091304-78091323 | MS.gene89168:CDS | 40.0% | |
| AGGGATGCTTCAGAACTAAA+AGG | - | chr8.1:78091269-78091288 | MS.gene89168:CDS | 40.0% | |
| AGGTAAAGTCGGATATAGTG+TGG | + | chr8.1:78090244-78090263 | None:intergenic | 40.0% | |
| AGGTCTTTATGTAATCCGTG+GGG | - | chr8.1:78089644-78089663 | MS.gene89168:intron | 40.0% | |
| AGTACGACTTATTAGGGATG+CGG | - | chr8.1:78090433-78090452 | MS.gene89168:intron | 40.0% | |
| ATAGGCACTTCTGCAATGAA+TGG | + | chr8.1:78089942-78089961 | None:intergenic | 40.0% | |
| CACTATATCCGACTTTACCT+TGG | - | chr8.1:78090244-78090263 | MS.gene89168:intron | 40.0% | |
| CGGATTACATAAAGACCTAG+AGG | + | chr8.1:78089642-78089661 | None:intergenic | 40.0% | |
| CTAGGTCTTTATGTAATCCG+TGG | - | chr8.1:78089642-78089661 | MS.gene89168:intron | 40.0% | |
| CTATATTGTGACATCCCTCT+AGG | - | chr8.1:78089624-78089643 | MS.gene89168:intron | 40.0% | |
| GATATTTCAGGCACAGAAAG+TGG | - | chr8.1:78091244-78091263 | MS.gene89168:intron | 40.0% | |
| GCAACGAGCTAGAAATAAAG+TGG | - | chr8.1:78090313-78090332 | MS.gene89168:intron | 40.0% | |
| GGGATGCTTCAGAACTAAAA+GGG | - | chr8.1:78091270-78091289 | MS.gene89168:CDS | 40.0% | |
| GGTAAAGTCGGATATAGTGT+GGG | + | chr8.1:78090243-78090262 | None:intergenic | 40.0% | |
| GTGTTCCTACAGAAGAAATC+AGG | - | chr8.1:78089814-78089833 | MS.gene89168:intron | 40.0% | |
| TCCTACAGAAGAAATCAGGA+GGG | - | chr8.1:78089818-78089837 | MS.gene89168:intron | 40.0% | |
| TTCCTACAGAAGAAATCAGG+AGG | - | chr8.1:78089817-78089836 | MS.gene89168:intron | 40.0% | |
| TTTCACTCTCTGATTCACCA+GGG | - | chr8.1:78090713-78090732 | MS.gene89168:intron | 40.0% | |
| ! | ACAGAATTGGCTTAGATAGG+AGG | - | chr8.1:78090132-78090151 | MS.gene89168:intron | 40.0% |
| !! | CAATTTTCAGGATGTGGAGA+GGG | - | chr8.1:78089767-78089786 | MS.gene89168:intron | 40.0% |
| !! | TCAATTTTCAGGATGTGGAG+AGG | - | chr8.1:78089766-78089785 | MS.gene89168:intron | 40.0% |
| AAACTCTTGCAGTGGATCCA+AGG | + | chr8.1:78090264-78090283 | None:intergenic | 45.0% | |
| ACCAACAATTACCCTCTCAC+AGG | + | chr8.1:78089601-78089620 | None:intergenic | 45.0% | |
| ACCCTCCTGATTTCTTCTGT+AGG | + | chr8.1:78089822-78089841 | None:intergenic | 45.0% | |
| ACGAGATGGTAGAAAGCTCA+TGG | - | chr8.1:78089449-78089468 | MS.gene89168:CDS | 45.0% | |
| ACTGCTAAAGCAAGTATCCC+TGG | + | chr8.1:78090733-78090752 | None:intergenic | 45.0% | |
| ACTTCTGCAATGAATGGCCA+CGG | + | chr8.1:78089936-78089955 | None:intergenic | 45.0% | |
| CAGTGGATCCAAGGTAAAGT+CGG | + | chr8.1:78090255-78090274 | None:intergenic | 45.0% | |
| CCAAGTTGCCTGTACTAGTA+TGG | - | chr8.1:78090291-78090310 | MS.gene89168:intron | 45.0% | |
| CCATACTAGTACAGGCAACT+TGG | + | chr8.1:78090294-78090313 | None:intergenic | 45.0% | |
| CGAGATGGTAGAAAGCTCAT+GGG | - | chr8.1:78089450-78089469 | MS.gene89168:CDS | 45.0% | |
| CTTAAGTGAGGGCAAGAACA+AGG | + | chr8.1:78089897-78089916 | None:intergenic | 45.0% | |
| CTTGTTCTTGCCCTCACTTA+AGG | - | chr8.1:78089895-78089914 | MS.gene89168:intron | 45.0% | |
| CTTTCACTCTCTGATTCACC+AGG | - | chr8.1:78090712-78090731 | MS.gene89168:intron | 45.0% | |
| GAGATGGTAGAAAGCTCATG+GGG | - | chr8.1:78089451-78089470 | MS.gene89168:CDS | 45.0% | |
| GTGCTGTAAAACTCTTGCAG+TGG | + | chr8.1:78090272-78090291 | None:intergenic | 45.0% | |
| TAGGAACACGCGTCATATGT+GGG | + | chr8.1:78089803-78089822 | None:intergenic | 45.0% | |
| TCAGGCACAGAAAGTGGAAA+GGG | - | chr8.1:78091250-78091269 | MS.gene89168:intron | 45.0% | |
| TTCAGGCACAGAAAGTGGAA+AGG | - | chr8.1:78091249-78091268 | MS.gene89168:intron | 45.0% | |
| AGGAACACGCGTCATATGTG+GGG | + | chr8.1:78089802-78089821 | None:intergenic | 50.0% | |
| GCAGCTAACGCTGTTCTTGA+GGG | - | chr8.1:78089573-78089592 | MS.gene89168:intron | 50.0% | |
| GCATGTCAAAACGGACGACA+TGG | + | chr8.1:78091160-78091179 | None:intergenic | 50.0% | |
| GCCTGTGAGAGGGTAATTGT+TGG | - | chr8.1:78089597-78089616 | MS.gene89168:intron | 50.0% | |
| GCTCGTTGCCATACTAGTAC+AGG | + | chr8.1:78090302-78090321 | None:intergenic | 50.0% | |
| GGCAATACAATGTTGTGCCG+TGG | - | chr8.1:78089916-78089935 | MS.gene89168:intron | 50.0% | |
| GTAGGAACACGCGTCATATG+TGG | + | chr8.1:78089804-78089823 | None:intergenic | 50.0% | |
| TGCAGCTAACGCTGTTCTTG+AGG | - | chr8.1:78089572-78089591 | MS.gene89168:intron | 50.0% | |
| !! | TTTTCAGGATGTGGAGAGGG+AGG | - | chr8.1:78089770-78089789 | MS.gene89168:intron | 50.0% |
| GGAACACGCGTCATATGTGG+GGG | + | chr8.1:78089801-78089820 | None:intergenic | 55.0% | |
| !! | TCTTGAGGGTGCCTGTGAGA+GGG | - | chr8.1:78089587-78089606 | MS.gene89168:intron | 55.0% |
| !! | TTCTTGAGGGTGCCTGTGAG+AGG | - | chr8.1:78089586-78089605 | MS.gene89168:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 78089430 | 78091331 | 78089430 | ID=MS.gene89168 |
| chr8.1 | mRNA | 78089430 | 78091331 | 78089430 | ID=MS.gene89168.t1;Parent=MS.gene89168 |
| chr8.1 | exon | 78091263 | 78091331 | 78091263 | ID=MS.gene89168.t1.exon1;Parent=MS.gene89168.t1 |
| chr8.1 | CDS | 78091263 | 78091331 | 78091263 | ID=cds.MS.gene89168.t1;Parent=MS.gene89168.t1 |
| chr8.1 | exon | 78091069 | 78091184 | 78091069 | ID=MS.gene89168.t1.exon2;Parent=MS.gene89168.t1 |
| chr8.1 | CDS | 78091069 | 78091184 | 78091069 | ID=cds.MS.gene89168.t1;Parent=MS.gene89168.t1 |
| chr8.1 | exon | 78090922 | 78090984 | 78090922 | ID=MS.gene89168.t1.exon3;Parent=MS.gene89168.t1 |
| chr8.1 | CDS | 78090922 | 78090984 | 78090922 | ID=cds.MS.gene89168.t1;Parent=MS.gene89168.t1 |
| chr8.1 | exon | 78089430 | 78089507 | 78089430 | ID=MS.gene89168.t1.exon4;Parent=MS.gene89168.t1 |
| chr8.1 | CDS | 78089430 | 78089507 | 78089430 | ID=cds.MS.gene89168.t1;Parent=MS.gene89168.t1 |
| Gene Sequence |
| Protein sequence |
