AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89188

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 63 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCAGCTGGAGTCAGATTT+TGG 0.184824 5.2:+66825030 MS.gene89188:CDS
GTCCTAATTTGCAGAATAAA+TGG 0.187586 5.2:+66827526 MS.gene89188:intron
AATTGGATCACAAAGTTAAA+TGG 0.272678 5.2:+66824951 MS.gene89188:CDS
TATGAGAAATTTGTGTATTT+AGG 0.278442 5.2:+66825064 MS.gene89188:CDS
ACCCGTACAGTCTTCTTCTT+AGG 0.292295 5.2:-66827585 None:intergenic
TTCAGCATCAATTCCAAATC+TGG 0.309760 5.2:-66827338 None:intergenic
TTTGGGCAGGCATTGGGCAT+TGG 0.319761 5.2:+66826859 MS.gene89188:intron
TCTACAGTGGACTCCAGATT+TGG 0.325328 5.2:+66827325 MS.gene89188:intron
TTGGGCAGGCATTGGGCATT+GGG 0.331705 5.2:+66826860 MS.gene89188:intron
GTTCGAAAATTGCTGTTAAG+AGG 0.349529 5.2:-66827800 None:intergenic
TTTCCATCGACAGACTTCTT+TGG 0.350193 5.2:+66827704 MS.gene89188:intron
GAAGACGCTGTGGCAGACTA+TGG 0.357365 5.2:+66824998 MS.gene89188:CDS
TTCTGCTGTGCCTGCTGTGA+TGG 0.363537 5.2:+66827758 MS.gene89188:CDS
TGTGTCTTTGGGCAGGCATT+GGG 0.368874 5.2:+66826853 MS.gene89188:intron
TAAATCAGAATCCATCCTTA+AGG 0.383863 5.2:+66827400 MS.gene89188:CDS
GAAGTCTGTGGCATTGGAAT+TGG 0.392646 5.2:+66824934 MS.gene89188:CDS
AATTTGTGTATTTAGGCATT+GGG 0.396459 5.2:+66825071 MS.gene89188:CDS
TCCTAATTTGCAGAATAAAT+GGG 0.404078 5.2:+66827527 MS.gene89188:intron
ACCAGGCTCAGCAGAAGTAC+TGG 0.405633 5.2:-66827733 None:intergenic
AAATTTGTGTATTTAGGCAT+TGG 0.429814 5.2:+66825070 MS.gene89188:CDS
GGGAAGCCCGTCAGCTAGCT+AGG 0.436166 5.2:+66826724 MS.gene89188:CDS
CCCAGTACTTCTGCTGAGCC+TGG 0.444702 5.2:+66827732 MS.gene89188:CDS
CAAGATGAAGTCTGTGGCAT+TGG 0.445821 5.2:+66824928 MS.gene89188:CDS
CTTGTCTGCTGCAGCTTAAG+TGG 0.445957 5.2:+66826664 MS.gene89188:intron
CTAGGTCTAATGCAGGGACT+CGG 0.461461 5.2:+66826742 MS.gene89188:CDS
AGCCAAAATCTGACTCCAGC+TGG 0.478708 5.2:-66825032 None:intergenic
GAGCAAAATCAACATCCTTA+AGG 0.503039 5.2:-66827415 None:intergenic
GCAGGCACAGCAGAAGTACC+AGG 0.511520 5.2:-66827750 None:intergenic
TAGGTCTAATGCAGGGACTC+GGG 0.513819 5.2:+66826743 MS.gene89188:CDS
TACTGCGAATGCAGGTATCA+AGG 0.530740 5.2:+66827830 MS.gene89188:CDS
CATTGGGGAACGTGAACTAA+AGG 0.533880 5.2:+66825087 MS.gene89188:CDS
CAGATGGTTACTGCGAATGC+AGG 0.537426 5.2:+66827822 MS.gene89188:CDS
ATCCTAAGAAGAAGACTGTA+CGG 0.543822 5.2:+66827583 MS.gene89188:CDS
ATCCTCCCCATGTATATCTA+CGG 0.546575 5.2:-66827366 None:intergenic
TTGTCTGCTGCAGCTTAAGT+GGG 0.549453 5.2:+66826665 MS.gene89188:intron
ATGGATCTGATGACTCCAGC+TGG 0.553415 5.2:+66825017 MS.gene89188:CDS
CAGCTAGCTAGGTCTAATGC+AGG 0.557805 5.2:+66826735 MS.gene89188:CDS
AAAAGCACAAAAGCTGAAGA+GGG 0.560388 5.2:+66826704 MS.gene89188:CDS
AAAGATGCCGTAGATATACA+TGG 0.565113 5.2:+66827359 MS.gene89188:CDS
GTTAAGAGGTTCACAACAGC+AGG 0.566554 5.2:-66827786 None:intergenic
CCTAATTTGCAGAATAAATG+GGG 0.566683 5.2:+66827528 MS.gene89188:intron
CCAGGCTCAGCAGAAGTACT+GGG 0.570943 5.2:-66827732 None:intergenic
CATCATGAGTCCATTTGTGA+AGG 0.572977 5.2:-66827439 None:intergenic
AAAATCAACATCCTTAAGGA+TGG 0.578151 5.2:-66827411 None:intergenic
GCAGGCACATCCATCACAGC+AGG 0.580554 5.2:-66827768 None:intergenic
CATTGGGAAACGTGAACCAA+AGG 0.594101 5.2:+66826876 MS.gene89188:CDS
ATCATGAGTCCATTTGTGAA+GGG 0.594860 5.2:-66827438 None:intergenic
TCCTAAGAAGAAGACTGTAC+GGG 0.599111 5.2:+66827584 MS.gene89188:CDS
ATGGACTCATGATGCACTGA+GGG 0.605994 5.2:+66827448 MS.gene89188:CDS
ATTTGTGTATTTAGGCATTG+GGG 0.609636 5.2:+66825072 MS.gene89188:CDS
GGTTAATGAAAACATCAGAA+AGG 0.612698 5.2:+66827605 MS.gene89188:CDS
AGGTCTAATGCAGGGACTCG+GGG 0.616145 5.2:+66826744 MS.gene89188:CDS
TGCCGTAGATATACATGGGG+AGG 0.620440 5.2:+66827364 MS.gene89188:CDS
GTCTCTGCTTGAAGACGCTG+TGG 0.621674 5.2:+66824988 MS.gene89188:CDS
TCATGAGTCCATTTGTGAAG+GGG 0.624080 5.2:-66827437 None:intergenic
CATTAGACCTAGCTAGCTGA+CGG 0.635381 5.2:-66826731 None:intergenic
AAGATGCCGTAGATATACAT+GGG 0.637221 5.2:+66827360 MS.gene89188:CDS
ATTAGACCTAGCTAGCTGAC+GGG 0.642091 5.2:-66826730 None:intergenic
AGCTAGCTAGGTCTAATGCA+GGG 0.656329 5.2:+66826736 MS.gene89188:CDS
CTAATTTGCAGAATAAATGG+GGG 0.682270 5.2:+66827529 MS.gene89188:intron
AGATCGCAAGATGAAGTCTG+TGG 0.683681 5.2:+66824922 MS.gene89188:CDS
AATGGACTCATGATGCACTG+AGG 0.724301 5.2:+66827447 MS.gene89188:CDS
AGATGCCGTAGATATACATG+GGG 0.731909 5.2:+66827361 MS.gene89188:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTATTTTTTACCTTT+GGG - chr5.2:66826788-66826807 None:intergenic 10.0%
!!! TTTATTTTATTTTTTACCTT+TGG - chr5.2:66826789-66826808 None:intergenic 10.0%
!! AATAAACAAACTACTATATA+TGG + chr5.2:66826803-66826822 MS.gene89188:intron 15.0%
!! AATTGTTATAGATTAATTAG+TGG + chr5.2:66826331-66826350 MS.gene89188:intron 15.0%
!!! ATAAGTTTTAAAAATACAGA+TGG + chr5.2:66827637-66827656 MS.gene89188:intron 15.0%
!!! TAAGTTTTAAAAATACAGAT+GGG + chr5.2:66827638-66827657 MS.gene89188:intron 15.0%
!!! TATTTTATTTTTTACCTTTG+GGG - chr5.2:66826787-66826806 None:intergenic 15.0%
!!! TTTACTTTTTATACATGAAT+TGG + chr5.2:66825153-66825172 MS.gene89188:intron 15.0%
!! TATAAAAAAGAACATAGCTT+GGG + chr5.2:66825459-66825478 MS.gene89188:intron 20.0%
!! TATGAGAAATTTGTGTATTT+AGG + chr5.2:66825064-66825083 MS.gene89188:CDS 20.0%
!! TTGAATTATGTGATAGAAAA+TGG + chr5.2:66827204-66827223 MS.gene89188:intron 20.0%
!!! AAATGTGTTTAAGATTGAAA+AGG - chr5.2:66825622-66825641 None:intergenic 20.0%
!!! AATAAAGCAAGTTAGTTTTT+TGG - chr5.2:66826482-66826501 None:intergenic 20.0%
!!! ACTAAAAAAAGATAATGGTT+GGG + chr5.2:66827554-66827573 MS.gene89188:CDS 20.0%
!!! ATTTTGTTATTGGAAAAGAT+TGG - chr5.2:66825838-66825857 None:intergenic 20.0%
!!! TAATATCGATTCAAAATTTG+AGG - chr5.2:66825269-66825288 None:intergenic 20.0%
!!! TATTTTCTTCTTCATAACAA+AGG - chr5.2:66825943-66825962 None:intergenic 20.0%
!!! TGAACAAGTTATTTTGTTAT+TGG - chr5.2:66825848-66825867 None:intergenic 20.0%
!!! TGGTTCTTAAATTTAGTTTA+TGG - chr5.2:66826027-66826046 None:intergenic 20.0%
! AAACAAACTATCTATCTATG+AGG + chr5.2:66827258-66827277 MS.gene89188:intron 25.0%
! AAATTTGTGTATTTAGGCAT+TGG + chr5.2:66825070-66825089 MS.gene89188:CDS 25.0%
! AACAAACTATCTATCTATGA+GGG + chr5.2:66827259-66827278 MS.gene89188:intron 25.0%
! AATTGGATCACAAAGTTAAA+TGG + chr5.2:66824951-66824970 MS.gene89188:CDS 25.0%
! AATTTGTGTATTTAGGCATT+GGG + chr5.2:66825071-66825090 MS.gene89188:CDS 25.0%
! AGAAAAACACTATAATGAGA+AGG - chr5.2:66827491-66827510 None:intergenic 25.0%
! ATATGAAAATTACATGAACC+TGG + chr5.2:66827064-66827083 MS.gene89188:intron 25.0%
! ATTGAATGAAAAATGAACAC+AGG + chr5.2:66826296-66826315 MS.gene89188:intron 25.0%
! ATTGTGTATAATAGCTAGAA+TGG - chr5.2:66825353-66825372 None:intergenic 25.0%
! GATTAAAATGCTATCTATGA+TGG + chr5.2:66827226-66827245 MS.gene89188:intron 25.0%
! GTATAAAAAAGAACATAGCT+TGG + chr5.2:66825458-66825477 MS.gene89188:intron 25.0%
! TGTGATTGATTAGAATGTTA+AGG + chr5.2:66826555-66826574 MS.gene89188:intron 25.0%
!! AAACTTTTTGAATTATGCCT+CGG + chr5.2:66826272-66826291 MS.gene89188:intron 25.0%
!! ATATGACATTTTGTTGCAAT+TGG - chr5.2:66825909-66825928 None:intergenic 25.0%
!! GACTAAAAAAAGATAATGGT+TGG + chr5.2:66827553-66827572 MS.gene89188:CDS 25.0%
!! GTAGTTTACTATTAGTTTGA+TGG + chr5.2:66826520-66826539 MS.gene89188:intron 25.0%
!! TAATCATTCATTTGTTGCAA+AGG + chr5.2:66825509-66825528 MS.gene89188:intron 25.0%
!! TATCTGTTCTGATTTTTACA+TGG + chr5.2:66827299-66827318 MS.gene89188:intron 25.0%
!! TCCTAATTTGCAGAATAAAT+GGG + chr5.2:66827527-66827546 MS.gene89188:intron 25.0%
!! TGTTCTAATTTTGTGTCTTT+GGG + chr5.2:66826842-66826861 MS.gene89188:intron 25.0%
!! TTGTTCTAATTTTGTGTCTT+TGG + chr5.2:66826841-66826860 MS.gene89188:intron 25.0%
!!! GTTTATGGTTTTTTTGTTGT+AGG - chr5.2:66826012-66826031 None:intergenic 25.0%
!!! TTATTTTTACCTTGCTCAAA+AGG - chr5.2:66827009-66827028 None:intergenic 25.0%
AAAATCAACATCCTTAAGGA+TGG - chr5.2:66827414-66827433 None:intergenic 30.0%
ACACTATAATGAGAAGGAAA+AGG - chr5.2:66827485-66827504 None:intergenic 30.0%
CTAACATAGTACTTTAACAG+AGG + chr5.2:66825716-66825735 MS.gene89188:intron 30.0%
CTACAGTGAAGAAATGAAAT+CGG - chr5.2:66825577-66825596 None:intergenic 30.0%
GCAACAAATGAATGATTAAC+AGG - chr5.2:66825507-66825526 None:intergenic 30.0%
GGGAGACTAAAAAAAGATAA+TGG + chr5.2:66827549-66827568 MS.gene89188:CDS 30.0%
GGTTAATGAAAACATCAGAA+AGG + chr5.2:66827605-66827624 MS.gene89188:CDS 30.0%
TAAATCAGAATCCATCCTTA+AGG + chr5.2:66827400-66827419 MS.gene89188:CDS 30.0%
TTCTTCTTCTTCTTCTTTGT+TGG - chr5.2:66826047-66826066 None:intergenic 30.0%
TTGTGATGATTAGAAGGTTA+AGG + chr5.2:66827163-66827182 MS.gene89188:intron 30.0%
TTTAAAAATACAGATGGGAC+TGG + chr5.2:66827643-66827662 MS.gene89188:intron 30.0%
! AAAAAGATAATGGTTGGGTT+AGG + chr5.2:66827559-66827578 MS.gene89188:CDS 30.0%
! ATAGCTAGAATGGATTCATT+TGG - chr5.2:66825343-66825362 None:intergenic 30.0%
! ATTTGTGTATTTAGGCATTG+GGG + chr5.2:66825072-66825091 MS.gene89188:CDS 30.0%
! CCTAATTTGCAGAATAAATG+GGG + chr5.2:66827528-66827547 MS.gene89188:intron 30.0%
! CTAATTTGCAGAATAAATGG+GGG + chr5.2:66827529-66827548 MS.gene89188:intron 30.0%
! GTCCTAATTTGCAGAATAAA+TGG + chr5.2:66827526-66827545 MS.gene89188:intron 30.0%
! TTTTACATGGTATTCTACAG+TGG + chr5.2:66827312-66827331 MS.gene89188:intron 30.0%
!! TCATTTTTCATTCAATACCG+AGG - chr5.2:66826292-66826311 None:intergenic 30.0%
!!! AAGTTAGTTTTTTGGCAAGT+TGG - chr5.2:66826474-66826493 None:intergenic 30.0%
!!! TCGCAGCAACATTTTTTTAT+AGG - chr5.2:66825429-66825448 None:intergenic 30.0%
AAAGATGCCGTAGATATACA+TGG + chr5.2:66827359-66827378 MS.gene89188:CDS 35.0%
AAGATGCCGTAGATATACAT+GGG + chr5.2:66827360-66827379 MS.gene89188:CDS 35.0%
AATCATTGAAAAAGCCCCAA+AGG + chr5.2:66826770-66826789 MS.gene89188:CDS 35.0%
AGAAGAGAGCAAATTACCTT+TGG - chr5.2:66826895-66826914 None:intergenic 35.0%
AGATTGGAAGAGATAAGGAT+CGG - chr5.2:66825822-66825841 None:intergenic 35.0%
AGGAGGATATTTGTTCTATG+TGG - chr5.2:66825784-66825803 None:intergenic 35.0%
ATCATGAGTCCATTTGTGAA+GGG - chr5.2:66827441-66827460 None:intergenic 35.0%
ATCCTAAGAAGAAGACTGTA+CGG + chr5.2:66827583-66827602 MS.gene89188:CDS 35.0%
CAAAGCTGGATATTTGTTCT+AGG - chr5.2:66825756-66825775 None:intergenic 35.0%
CCCCATTTATTCTGCAAATT+AGG - chr5.2:66827531-66827550 None:intergenic 35.0%
GAGCAAAATCAACATCCTTA+AGG - chr5.2:66827418-66827437 None:intergenic 35.0%
GGAAAAGATTGGAAGAGATA+AGG - chr5.2:66825827-66825846 None:intergenic 35.0%
TTCAGCATCAATTCCAAATC+TGG - chr5.2:66827341-66827360 None:intergenic 35.0%
! AAAAAGCACAAAAGCTGAAG+AGG + chr5.2:66826703-66826722 MS.gene89188:CDS 35.0%
! AAAAGCACAAAAGCTGAAGA+GGG + chr5.2:66826704-66826723 MS.gene89188:CDS 35.0%
! AACAGCAATTTTCGAACAGA+TGG + chr5.2:66827806-66827825 MS.gene89188:CDS 35.0%
! ATGGATTCATTTGGTGATAG+AGG - chr5.2:66825334-66825353 None:intergenic 35.0%
! GACATTTTGTTGCAATTGGA+GGG - chr5.2:66825905-66825924 None:intergenic 35.0%
! GGAAATTGTGACTCTTTTGA+AGG + chr5.2:66827851-66827870 MS.gene89188:CDS 35.0%
! GTTCGAAAATTGCTGTTAAG+AGG - chr5.2:66827803-66827822 None:intergenic 35.0%
! TGACATTTTGTTGCAATTGG+AGG - chr5.2:66825906-66825925 None:intergenic 35.0%
! TGCTGTTTGTGATGATTAGA+AGG + chr5.2:66827157-66827176 MS.gene89188:intron 35.0%
! TTATTCATGCCTTTTGAGCA+AGG + chr5.2:66826997-66827016 MS.gene89188:intron 35.0%
! TTGAAAAGGAAAAGCACACA+TGG - chr5.2:66825608-66825627 None:intergenic 35.0%
! TTGGGTTGTTTGAGTAAGTT+AGG + chr5.2:66825477-66825496 MS.gene89188:intron 35.0%
! TTTTCATTATCTCCCAAACC+TGG + chr5.2:66825375-66825394 MS.gene89188:intron 35.0%
! TTTTCTCTATCTCCAGGTTT+GGG - chr5.2:66825390-66825409 None:intergenic 35.0%
AAGATGCCAAAGGTAGAGAT+AGG + chr5.2:66826065-66826084 MS.gene89188:intron 40.0%
AGAAGAAGAAGAAGCTACAC+TGG + chr5.2:66826116-66826135 MS.gene89188:intron 40.0%
AGATGCCGTAGATATACATG+GGG + chr5.2:66827361-66827380 MS.gene89188:CDS 40.0%
ATCCTCCCCATGTATATCTA+CGG - chr5.2:66827369-66827388 None:intergenic 40.0%
CATCATGAGTCCATTTGTGA+AGG - chr5.2:66827442-66827461 None:intergenic 40.0%
GAAGAAGAAGAAGATGCCAA+AGG + chr5.2:66826055-66826074 MS.gene89188:intron 40.0%
TCAACGTTGTATGGTGTGAA+TGG + chr5.2:66826142-66826161 MS.gene89188:intron 40.0%
TCATGAGTCCATTTGTGAAG+GGG - chr5.2:66827440-66827459 None:intergenic 40.0%
TCCTAAGAAGAAGACTGTAC+GGG + chr5.2:66827584-66827603 MS.gene89188:CDS 40.0%
TTTCCATCGACAGACTTCTT+TGG + chr5.2:66827704-66827723 MS.gene89188:intron 40.0%
! ATTTTGCTCCCCTTCACAAA+TGG + chr5.2:66827429-66827448 MS.gene89188:CDS 40.0%
! CTAATTTTGTGTCTTTGGGC+AGG + chr5.2:66826846-66826865 MS.gene89188:intron 40.0%
! CTTTTCTCTATCTCCAGGTT+TGG - chr5.2:66825391-66825410 None:intergenic 40.0%
! TTGAGCTTTTCTCTATCTCC+AGG - chr5.2:66825396-66825415 None:intergenic 40.0%
!!! CACTGGTTTTCAACGTTGTA+TGG + chr5.2:66826133-66826152 MS.gene89188:intron 40.0%
ACCCGTACAGTCTTCTTCTT+AGG - chr5.2:66827588-66827607 None:intergenic 45.0%
ACCTGGCTACATGAACTTGA+AGG + chr5.2:66827081-66827100 MS.gene89188:intron 45.0%
ACGTTGTATGGTGTGAATGG+AGG + chr5.2:66826145-66826164 MS.gene89188:intron 45.0%
AGATCGCAAGATGAAGTCTG+TGG + chr5.2:66824922-66824941 MS.gene89188:CDS 45.0%
AGCTAGCTAGGTCTAATGCA+GGG + chr5.2:66826736-66826755 MS.gene89188:CDS 45.0%
ATGCCAAAGGTAGAGATAGG+TGG + chr5.2:66826068-66826087 MS.gene89188:intron 45.0%
ATGGACTCATGATGCACTGA+GGG + chr5.2:66827448-66827467 MS.gene89188:CDS 45.0%
ATGGTGTGAATGGAGGGAAA+TGG + chr5.2:66826152-66826171 MS.gene89188:intron 45.0%
ATTAGACCTAGCTAGCTGAC+GGG - chr5.2:66826733-66826752 None:intergenic 45.0%
CAAGATGAAGTCTGTGGCAT+TGG + chr5.2:66824928-66824947 MS.gene89188:CDS 45.0%
CATCCAAAGAAGTCTGTCGA+TGG - chr5.2:66827710-66827729 None:intergenic 45.0%
CATTAGACCTAGCTAGCTGA+CGG - chr5.2:66826734-66826753 None:intergenic 45.0%
CATTGGGAAACGTGAACCAA+AGG + chr5.2:66826876-66826895 MS.gene89188:CDS 45.0%
CATTGGGGAACGTGAACTAA+AGG + chr5.2:66825087-66825106 MS.gene89188:CDS 45.0%
CCTGGCTACATGAACTTGAA+GGG + chr5.2:66827082-66827101 MS.gene89188:intron 45.0%
CGTTGTATGGTGTGAATGGA+GGG + chr5.2:66826146-66826165 MS.gene89188:intron 45.0%
GTTAAGAGGTTCACAACAGC+AGG - chr5.2:66827789-66827808 None:intergenic 45.0%
TACTGCGAATGCAGGTATCA+AGG + chr5.2:66827830-66827849 MS.gene89188:CDS 45.0%
TCTATGTGGTGACACAAAGC+TGG - chr5.2:66825770-66825789 None:intergenic 45.0%
TTGTCTGCTGCAGCTTAAGT+GGG + chr5.2:66826665-66826684 MS.gene89188:intron 45.0%
! AATACAGATGGGACTGGTAC+TGG + chr5.2:66827649-66827668 MS.gene89188:intron 45.0%
! AATGGACTCATGATGCACTG+AGG + chr5.2:66827447-66827466 MS.gene89188:CDS 45.0%
! GAAGTCTGTGGCATTGGAAT+TGG + chr5.2:66824934-66824953 MS.gene89188:CDS 45.0%
! TCTACAGTGGACTCCAGATT+TGG + chr5.2:66827325-66827344 MS.gene89188:intron 45.0%
AGCCAAAATCTGACTCCAGC+TGG - chr5.2:66825035-66825054 None:intergenic 50.0%
ATGGATCTGATGACTCCAGC+TGG + chr5.2:66825017-66825036 MS.gene89188:CDS 50.0%
CACTTAAGCTGCAGCAGACA+AGG - chr5.2:66826666-66826685 None:intergenic 50.0%
CAGATGGTTACTGCGAATGC+AGG + chr5.2:66827822-66827841 MS.gene89188:CDS 50.0%
CAGCTAGCTAGGTCTAATGC+AGG + chr5.2:66826735-66826754 MS.gene89188:CDS 50.0%
CCAAAGGTAGAGATAGGTGG+TGG + chr5.2:66826071-66826090 MS.gene89188:intron 50.0%
CCACCACCTATCTCTACCTT+TGG - chr5.2:66826074-66826093 None:intergenic 50.0%
CTAGGTCTAATGCAGGGACT+CGG + chr5.2:66826742-66826761 MS.gene89188:CDS 50.0%
CTTGTCTGCTGCAGCTTAAG+TGG + chr5.2:66826664-66826683 MS.gene89188:intron 50.0%
GGAAGAGATAAGGATCGGCT+GGG - chr5.2:66825817-66825836 None:intergenic 50.0%
TAGGTCTAATGCAGGGACTC+GGG + chr5.2:66826743-66826762 MS.gene89188:CDS 50.0%
TGCCGTAGATATACATGGGG+AGG + chr5.2:66827364-66827383 MS.gene89188:CDS 50.0%
TGGAAGAGATAAGGATCGGC+TGG - chr5.2:66825818-66825837 None:intergenic 50.0%
TGTGTCTTTGGGCAGGCATT+GGG + chr5.2:66826853-66826872 MS.gene89188:intron 50.0%
TTGTGTCTTTGGGCAGGCAT+TGG + chr5.2:66826852-66826871 MS.gene89188:intron 50.0%
! AAGGTAGAGATAGGTGGTGG+AGG + chr5.2:66826074-66826093 MS.gene89188:intron 50.0%
! ATCGGCTGGGTTGACAAAGA+AGG - chr5.2:66825804-66825823 None:intergenic 50.0%
! CCCTTCAAGTTCATGTAGCC+AGG - chr5.2:66827085-66827104 None:intergenic 50.0%
! CTCCAGCTGGAGTCAGATTT+TGG + chr5.2:66825030-66825049 MS.gene89188:CDS 50.0%
AGGTCTAATGCAGGGACTCG+GGG + chr5.2:66826744-66826763 MS.gene89188:CDS 55.0%
GAAGACGCTGTGGCAGACTA+TGG + chr5.2:66824998-66825017 MS.gene89188:CDS 55.0%
GTCTCTGCTTGAAGACGCTG+TGG + chr5.2:66824988-66825007 MS.gene89188:CDS 55.0%
TTCTGCTGTGCCTGCTGTGA+TGG + chr5.2:66827758-66827777 MS.gene89188:CDS 55.0%
! ACCAGGCTCAGCAGAAGTAC+TGG - chr5.2:66827736-66827755 None:intergenic 55.0%
! GGCTGGGTTGACAAAGAAGG+AGG - chr5.2:66825801-66825820 None:intergenic 55.0%
!! CCAGGCTCAGCAGAAGTACT+GGG - chr5.2:66827735-66827754 None:intergenic 55.0%
!! GTAGAGATAGGTGGTGGAGG+AGG + chr5.2:66826077-66826096 MS.gene89188:intron 55.0%
!! TTGGGCAGGCATTGGGCATT+GGG + chr5.2:66826860-66826879 MS.gene89188:intron 55.0%
!! TTTGGGCAGGCATTGGGCAT+TGG + chr5.2:66826859-66826878 MS.gene89188:intron 55.0%
CCCAGTACTTCTGCTGAGCC+TGG + chr5.2:66827732-66827751 MS.gene89188:CDS 60.0%
GCAGGCACATCCATCACAGC+AGG - chr5.2:66827771-66827790 None:intergenic 60.0%
!! GAGATAGGTGGTGGAGGAGG+AGG + chr5.2:66826080-66826099 MS.gene89188:intron 60.0%
!! GCAGGCACAGCAGAAGTACC+AGG - chr5.2:66827753-66827772 None:intergenic 60.0%
GGGAAGCCCGTCAGCTAGCT+AGG + chr5.2:66826724-66826743 MS.gene89188:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 66824917 66827884 66824917 ID=MS.gene89188
chr5.2 mRNA 66824917 66827884 66824917 ID=MS.gene89188.t1;Parent=MS.gene89188
chr5.2 exon 66824917 66825108 66824917 ID=MS.gene89188.t1.exon1;Parent=MS.gene89188.t1
chr5.2 CDS 66824917 66825108 66824917 ID=cds.MS.gene89188.t1;Parent=MS.gene89188.t1
chr5.2 exon 66826678 66826791 66826678 ID=MS.gene89188.t1.exon2;Parent=MS.gene89188.t1
chr5.2 CDS 66826678 66826791 66826678 ID=cds.MS.gene89188.t1;Parent=MS.gene89188.t1
chr5.2 exon 66826868 66826897 66826868 ID=MS.gene89188.t1.exon3;Parent=MS.gene89188.t1
chr5.2 CDS 66826868 66826897 66826868 ID=cds.MS.gene89188.t1;Parent=MS.gene89188.t1
chr5.2 exon 66827332 66827469 66827332 ID=MS.gene89188.t1.exon4;Parent=MS.gene89188.t1
chr5.2 CDS 66827332 66827469 66827332 ID=cds.MS.gene89188.t1;Parent=MS.gene89188.t1
chr5.2 exon 66827540 66827626 66827540 ID=MS.gene89188.t1.exon5;Parent=MS.gene89188.t1
chr5.2 CDS 66827540 66827626 66827540 ID=cds.MS.gene89188.t1;Parent=MS.gene89188.t1
chr5.2 exon 66827717 66827884 66827717 ID=MS.gene89188.t1.exon6;Parent=MS.gene89188.t1
chr5.2 CDS 66827717 66827884 66827717 ID=cds.MS.gene89188.t1;Parent=MS.gene89188.t1
Gene Sequence

>MS.gene89188

ATGAAAGATCGCAAGATGAAGTCTGTGGCATTGGAATTGGATCACAAAGTTAAATGGAGCACGCTTAGAGAGTCTCTGCTTGAAGACGCTGTGGCAGACTATGGATCTGATGACTCCAGCTGGAGTCAGATTTTGGCTGATGAGAAGTATGAGAAATTTGTGTATTTAGGCATTGGGGAACGTGAACTAAAGGTAATTTGCTTTCTTCTTGCAATTCTGTTATTGCTATACTTACTTTTACTTTTTATACATGAATTGGTGTGAATGTGTTTTGAGATGTAGTGATACTTTTTATTCATATATGACATTAGTGTACTTACTTTTTAGTATATCAGTCTGCAAGCAAGAACCTCAAATTTTGAATCGATATTAGTGAGATGAACTTGCATACAAAAGCTTAAAAATTATAATTTTCCTCTATCACCAAATGAATCCATTCTAGCTATTATACACAATTTTTTTCATTATCTCCCAAACCTGGAGATAGAGAAAAGCTCAATTAGCAAAATCCTATAAAAAAATGTTGCTGCGACTAAACTATGTATAAAAAAGAACATAGCTTGGGTTGTTTGAGTAAGTTAGGAAAGCCTGTTAATCATTCATTTGTTGCAAAGGTTTGTGTTTTTAAATTGATTTATCTTTTAAATTATATAAGCGCCGATTTCATTTCTTCACTGTAGTATATCAGCCATGTGTGCTTTTCCTTTTCAATCTTAAACACATTTGCTTTTAGAATTATGTGAAACATATATACAAATTTGAAGAAGAACATAAGTAATGACATAAAAATATACAAAGTCTAACATAGTACTTTAACAGAGGAATTGTGCATTTCTCCTAGAACAAATATCCAGCTTTGTGTCACCACATAGAACAAATATCCTCCTTCTTTGTCAACCCAGCCGATCCTTATCTCTTCCAATCTTTTCCAATAACAAAATAACTTGTTCATGACTATTTTATGAAGAAGAAATCTCACTTTTGTCCCTCCAATTGCAACAAAATGTCATATTTTCAATGTCACCTTTGTTATGAAGAAGAAAATAAAATGATTAGAAAAAGTGAAGAAGAAACTGAACTCTGATAGTCTCTCCTACAACAAAAAAACCATAAACTAAATTTAAGAACCAACAAAGAAGAAGAAGAAGAAGATGCCAAAGGTAGAGATAGGTGGTGGAGGAGGAGGAGAATGATGAGAGAGAAGAAGAAGAAGCTACACTGGTTTTCAACGTTGTATGGTGTGAATGGAGGGAAATGGAGATAAGAAAGAAAAAAAAAAAACTTAAAACTTGAAATGTGAGAAGATTGAAATACAATTTTTTGTGTTCAAAGAAACAAAATGAATTCATTGATACAAACTTTTTGAATTATGCCTCGGTATTGAATGAAAAATGAACACAGGCATTGAAAATATAATTGTTATAGATTAATTAGTGGAGTAATCAATAAAAGATAAATATACAAATAAAAAATAATTGAAACAAATAATTATAGATTAATTACTATTAATAGAAAGTTTAGAAGAGCTAAGTACTCTAATTGATTGACAAATGTCCAACTTGCCAAAAAACTAACTTGCTTTATTATTATCTATAGATAGATAGTAGTTTACTATTAGTTTGATAGAATTTTGTTGTTTGTGATTGATTAGAATGTTAAGGTTCATAGATAGAGATAAGTTGAATTATTTGATAGAGAATGAATTAAAATGCTTTTTTTTACTAGTATATATTTTACTTTCACTGTCCTTGTCTGCTGCAGCTTAAGTGGGAAGCTTCTGAGAAGAAAAAAGCACAAAAGCTGAAGAGGGAAGCCCGTCAGCTAGCTAGGTCTAATGCAGGGACTCGGGGTGTAATCATTGAAAAAGCCCCAAAGGTAAAAAATAAAATAAACAAACTACTATATATGGATTAAAGCAAACTATTTGTTCTAATTTTGTGTCTTTGGGCAGGCATTGGGCATTGGGAAACGTGAACCAAAGGTAATTTGCTCTCTTCTTGCAATTCTGTTATTGCTCTACTTATTTTCACTTTTTATTCATGATATGACTAGTACTTAAGATATAAATAGTACTTACTTTTTATTCATGCCTTTTGAGCAAGGTAAAAATAATTAAGATATAAATAGTACTTGCTTTTTATTCATGAATATGAAAATTACATGAACCTGGCTACATGAACTTGAAGGGAAGTTCATTGTTAAGATGTAAATAGTAGTTTACTATTAGTTTGATGGAATTTTGCTGTTTGTGATGATTAGAAGGTTAAGGTTCATAGATAGAGATAAGTTGAATTATGTGATAGAAAATGGATTAAAATGCTATCTATGATGGATTAAAAATAAACAAACTATCTATCTATGAGGGATTAGAAATAAACAAACTATCTGTTCTGATTTTTACATGGTATTCTACAGTGGACTCCAGATTTGGAATTGATGCTGAAAGATGCCGTAGATATACATGGGGAGGATCATGCTGATTTTAAATCAGAATCCATCCTTAAGGATGTTGATTTTGCTCCCCTTCACAAATGGACTCATGATGCACTGAGGGTTTGTATCTGTCCTTTTCCTTCTCATTATAGTGTTTTTCTTTAATTTAATAATAAGTCCTAATTTGCAGAATAAATGGGGGAGACTAAAAAAAGATAATGGTTGGGTTAGGAATCCTAAGAAGAAGACTGTACGGGTTAATGAAAACATCAGAAAGGTACGCTTAAATAAGTTTTAAAAATACAGATGGGACTGGTACTGGTACTTCTGATGTAAAGTTTTATTGTGTTGCTTTTTCCATCGACAGACTTCTTTGGATGTGCCCAGTACTTCTGCTGAGCCTGGTACTTCTGCTGTGCCTGCTGTGATGGATGTGCCTGCTGTTGTGAACCTCTTAACAGCAATTTTCGAACAGATGGTTACTGCGAATGCAGGTATCAAGGAAATTGTGACTCTTTTGAAGGATAGAAAGTGA
Protein sequence

>MS.gene89188.t1

MKDRKMKSVALELDHKVKWSTLRESLLEDAVADYGSDDSSWSQILADEKYEKFVYLGIGERELKLKWEASEKKKAQKLKREARQLARSNAGTRGVIIEKAPKALGIGKREPKWTPDLELMLKDAVDIHGEDHADFKSESILKDVDFAPLHKWTHDALRNKWGRLKKDNGWVRNPKKKTVRVNENIRKTSLDVPSTSAEPGTSAVPAVMDVPAVVNLLTAIFEQMVTANAGIKEIVTLLKDRK