Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90096.t1 | XP_013453265.1 | 99.3 | 1109 | 7 | 1 | 23 | 1131 | 95 | 1202 | 0.00E+00 | 2124.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90096.t1 | Q6ZY51 | 64.7 | 1136 | 366 | 5 | 16 | 1130 | 74 | 1195 | 0.0e+00 | 1462.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90096.t1 | A0A072UBU6 | 99.3 | 1109 | 7 | 1 | 23 | 1131 | 95 | 1202 | 0.0e+00 | 2124.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051360 | MS.gene90096 | 0.801031 | 1.09E-48 | -1.69E-46 |
| MS.gene05601 | MS.gene90096 | 0.802272 | 6.07E-49 | -1.69E-46 |
| MS.gene058948 | MS.gene90096 | 0.802386 | 5.75E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene90096 | 0.807057 | 6.09E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene90096 | 0.825891 | 3.66E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90096.t1 | MTR_6g093240 | 98.320 | 1131 | 16 | 2 | 3 | 1131 | 73 | 1202 | 0.0 | 2291 |
| MS.gene90096.t1 | MTR_6g093240 | 99.216 | 893 | 7 | 0 | 239 | 1131 | 1 | 893 | 0.0 | 1813 |
| MS.gene90096.t1 | MTR_1g117000 | 92.444 | 900 | 25 | 2 | 232 | 1131 | 370 | 1226 | 0.0 | 1667 |
| MS.gene90096.t1 | MTR_6g093240 | 99.071 | 646 | 6 | 0 | 486 | 1131 | 6 | 651 | 0.0 | 1298 |
| MS.gene90096.t1 | MTR_8g069760 | 27.825 | 593 | 345 | 17 | 565 | 1129 | 945 | 1482 | 1.13e-45 | 180 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90096.t1 | AT5G26570 | 65.328 | 1145 | 366 | 7 | 3 | 1130 | 65 | 1195 | 0.0 | 1524 |
| MS.gene90096.t1 | AT5G26570 | 66.138 | 756 | 241 | 5 | 3 | 757 | 65 | 806 | 0.0 | 1038 |
| MS.gene90096.t1 | AT1G10760 | 27.861 | 603 | 336 | 20 | 562 | 1129 | 860 | 1398 | 1.34e-43 | 174 |
| MS.gene90096.t1 | AT1G10760 | 27.861 | 603 | 336 | 20 | 562 | 1129 | 860 | 1398 | 1.34e-43 | 174 |
| MS.gene90096.t1 | AT1G10760 | 27.861 | 603 | 336 | 20 | 562 | 1129 | 860 | 1398 | 1.34e-43 | 174 |
| MS.gene90096.t1 | AT4G24450 | 25.845 | 592 | 353 | 16 | 565 | 1129 | 745 | 1277 | 3.62e-43 | 172 |
| MS.gene90096.t1 | AT4G24450 | 25.845 | 592 | 353 | 16 | 565 | 1129 | 745 | 1277 | 3.62e-43 | 172 |
| MS.gene90096.t1 | AT4G24450 | 25.845 | 592 | 353 | 16 | 565 | 1129 | 745 | 1277 | 3.62e-43 | 172 |
Find 302 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCATTGTCTTCAGTTTC+TGG | 0.094656 | 1.4:-88600830 | MS.gene90096:CDS |
| GCTTCCTTTCAGGTTCCTTC+TGG | 0.155466 | 1.4:-88600366 | MS.gene90096:CDS |
| CCACTTGGAGCCCTTATTAT+TGG | 0.185336 | 1.4:-88604102 | MS.gene90096:CDS |
| CCCAAGTCCTTTGTGTTTCA+TGG | 0.188668 | 1.4:+88609440 | None:intergenic |
| GCTGATATTCAAAGGCTTAT+TGG | 0.194561 | 1.4:-88601952 | MS.gene90096:CDS |
| ATTACCTACCTGACTATATT+TGG | 0.196451 | 1.4:+88601629 | None:intergenic |
| CAGATGCCATTGGCCAAGTA+TGG | 0.234162 | 1.4:-88600023 | MS.gene90096:CDS |
| TGAAATATTCAAGTCTTTAC+TGG | 0.249927 | 1.4:-88600289 | MS.gene90096:CDS |
| TCTGGTGGACTCCAATAATA+AGG | 0.251775 | 1.4:+88604091 | None:intergenic |
| GGTGACCATGTTGTGCTTCT+TGG | 0.252280 | 1.4:-88609895 | MS.gene90096:CDS |
| TCCTCTTCAGTTGACCTTGA+TGG | 0.269108 | 1.4:-88601729 | MS.gene90096:CDS |
| CTGATATTCAAAGGCTTATT+GGG | 0.271077 | 1.4:-88601951 | MS.gene90096:CDS |
| TTCATATTTCTACTGGGGTT+GGG | 0.275376 | 1.4:+88599498 | None:intergenic |
| CCAAGTTGTCGACGGAAAAC+TGG | 0.278406 | 1.4:+88599628 | None:intergenic |
| CTTCCTTTCAGGTTCCTTCT+GGG | 0.279361 | 1.4:-88600365 | MS.gene90096:CDS |
| ACTTATCTCATCTAGGTGTT+AGG | 0.288791 | 1.4:-88602124 | MS.gene90096:CDS |
| CGCATAGCTATTGCAGAATC+TGG | 0.289459 | 1.4:+88604818 | None:intergenic |
| TTGTTAACAATGAGGATAAT+AGG | 0.290979 | 1.4:+88602296 | None:intergenic |
| GCTGAGATTGCTTCTGGCCT+TGG | 0.293888 | 1.4:-88599838 | MS.gene90096:CDS |
| GTGGAGGCTGAGATTGCTTC+TGG | 0.297947 | 1.4:-88599844 | MS.gene90096:CDS |
| ATGAAGATGGCAAGAAGATT+TGG | 0.303471 | 1.4:-88603870 | MS.gene90096:CDS |
| CCACCCATGTTCCCTTGAAT+TGG | 0.305119 | 1.4:-88609846 | MS.gene90096:CDS |
| TGAAATTTCAGCATGCCTAT+TGG | 0.325389 | 1.4:+88607573 | None:intergenic |
| CAAGATAGAAACAGCAAAAC+TGG | 0.333922 | 1.4:-88600265 | MS.gene90096:CDS |
| TATGAATCTATGGATGAATA+TGG | 0.337230 | 1.4:-88605359 | MS.gene90096:CDS |
| ACCATTTGGATCCATGGAAT+TGG | 0.338771 | 1.4:-88600334 | MS.gene90096:CDS |
| TCATATTTCTACTGGGGTTG+GGG | 0.340681 | 1.4:+88599499 | None:intergenic |
| TCAATAGCACCACATTCTTC+TGG | 0.340741 | 1.4:+88604048 | None:intergenic |
| TCTCAACGTGCTTGAAGTTA+TGG | 0.341691 | 1.4:-88604184 | MS.gene90096:CDS |
| ATTAATGTCTTCTTTGTTGT+TGG | 0.342523 | 1.4:+88609957 | None:intergenic |
| CTCAACGTGCTTGAAGTTAT+GGG | 0.344693 | 1.4:-88604183 | MS.gene90096:CDS |
| ACCAGATGAAATTATCTAAC+TGG | 0.345548 | 1.4:-88604074 | MS.gene90096:CDS |
| CGGTGCAGGGCCTGCGGATG+GGG | 0.349145 | 1.4:-88599701 | MS.gene90096:CDS |
| GAAATTTCAGCATGCCTATT+GGG | 0.352024 | 1.4:+88607574 | None:intergenic |
| GCGGTGCAGGGCCTGCGGAT+GGG | 0.354005 | 1.4:-88599702 | MS.gene90096:CDS |
| CGCCGGCAGGTAACAGCTGC+TGG | 0.356703 | 1.4:-88602188 | MS.gene90096:intron |
| CAGTGGCGCCTCTGTGAGAT+TGG | 0.358646 | 1.4:-88604702 | MS.gene90096:CDS |
| GATCTGGTGAAAGCATTTCT+TGG | 0.362729 | 1.4:+88599917 | None:intergenic |
| AGAATCAATATATGAATCTA+TGG | 0.365446 | 1.4:-88605369 | MS.gene90096:CDS |
| ATATCTCTGATTCGAGTTAG+AGG | 0.376143 | 1.4:+88607227 | None:intergenic |
| TTGAGAGATATTATTGTAAA+GGG | 0.378655 | 1.4:-88604869 | MS.gene90096:CDS |
| TAGCTGGCTAGCTGCAAATT+TGG | 0.378739 | 1.4:-88604154 | MS.gene90096:CDS |
| GAGTTGCTTGTACGCGGTGC+AGG | 0.381894 | 1.4:-88599715 | MS.gene90096:CDS |
| CGCGGTGCAGGGCCTGCGGA+TGG | 0.382100 | 1.4:-88599703 | MS.gene90096:CDS |
| TGGGATACCTCAGGTCTTCA+AGG | 0.383554 | 1.4:-88609421 | MS.gene90096:CDS |
| GGCACTTTACACTGGGAATC+TGG | 0.387828 | 1.4:-88609742 | MS.gene90096:CDS |
| GATGTTCCTGCTTTCTCTGC+TGG | 0.389751 | 1.4:-88601660 | MS.gene90096:CDS |
| GGTTGGGATGTCATAGTTCC+AGG | 0.393806 | 1.4:-88603068 | MS.gene90096:CDS |
| ATTCATATTTCTACTGGGGT+TGG | 0.394846 | 1.4:+88599497 | None:intergenic |
| GAACAAAACACACCAGACTA+TGG | 0.395168 | 1.4:-88609662 | MS.gene90096:CDS |
| GGTCCAGGAGCCCAACTGTT+TGG | 0.395487 | 1.4:+88609867 | None:intergenic |
| CTTATCTCATCTAGGTGTTA+GGG | 0.411036 | 1.4:-88602123 | MS.gene90096:CDS |
| TGACCAGAATAATAATGATC+AGG | 0.411622 | 1.4:-88609581 | MS.gene90096:CDS |
| GATATAGTCGGAAGTGTTCA+TGG | 0.414405 | 1.4:-88607786 | MS.gene90096:CDS |
| GGTGATCGCCGGCGTTGAAA+TGG | 0.414806 | 1.4:+88609797 | None:intergenic |
| GGGATCAAAGCGCAAGGAAT+TGG | 0.415864 | 1.4:-88609375 | MS.gene90096:CDS |
| GGCTTATTGGGTCATGTGTA+AGG | 0.417870 | 1.4:-88601939 | MS.gene90096:intron |
| GTGTTGAGCCGCCGAGCTGC+TGG | 0.419748 | 1.4:-88599976 | MS.gene90096:CDS |
| TTGCCTCTCAAGTTTGTTCA+AGG | 0.420915 | 1.4:-88609397 | MS.gene90096:CDS |
| CCAAGTCCTTTGTGTTTCAT+GGG | 0.422081 | 1.4:+88609441 | None:intergenic |
| TCCAGTTAGATAATTTCATC+TGG | 0.425951 | 1.4:+88604073 | None:intergenic |
| TACGCTTGGTTCTCAAGGTT+GGG | 0.427931 | 1.4:-88603084 | MS.gene90096:CDS |
| AATGACAGTTTACAGCGATC+AGG | 0.429924 | 1.4:-88600397 | MS.gene90096:intron |
| CAACCAAACAGTTGGGCTCC+TGG | 0.430043 | 1.4:-88609870 | MS.gene90096:CDS |
| ACCATTTGGTGTCCAATTCA+AGG | 0.433494 | 1.4:+88609834 | None:intergenic |
| TCCATGTTTGCCTTCATTCA+AGG | 0.434190 | 1.4:-88607270 | MS.gene90096:CDS |
| TTGTCTTCAGTTTCTGGTAA+AGG | 0.434711 | 1.4:-88600824 | MS.gene90096:intron |
| CTCAAAGACTTCTTTAATGC+TGG | 0.438334 | 1.4:-88606895 | MS.gene90096:CDS |
| TGTTCATGGGGAAGATCAAT+TGG | 0.439782 | 1.4:-88607772 | MS.gene90096:CDS |
| CTTTATATGTTGCAGATACT+AGG | 0.440528 | 1.4:-88603399 | MS.gene90096:intron |
| GATGCTGAGACACAGACTTC+CGG | 0.440912 | 1.4:-88600881 | MS.gene90096:CDS |
| CAGCGCCAATGTGGAGGACT+TGG | 0.443634 | 1.4:-88600115 | MS.gene90096:CDS |
| GGTCAACGCCTTTGTGCAGT+TGG | 0.444213 | 1.4:-88599607 | MS.gene90096:CDS |
| ATATCATGTGGGAAATTTGA+TGG | 0.446585 | 1.4:-88599775 | MS.gene90096:CDS |
| AATTGTTATCCTGGTCAGTT+GGG | 0.449370 | 1.4:+88599869 | None:intergenic |
| AGTCAAAGAATGTAAACTCT+TGG | 0.452927 | 1.4:-88604131 | MS.gene90096:CDS |
| TAGGTTGAAAGAATTGTTCC+TGG | 0.453289 | 1.4:-88602344 | MS.gene90096:CDS |
| GAGGTCACCACCGTCCCAAT+AGG | 0.453384 | 1.4:-88607588 | MS.gene90096:CDS |
| CATGGTCACCGAATTGAACT+TGG | 0.453626 | 1.4:+88609908 | None:intergenic |
| CAACAGAAGAATTGCCATCA+AGG | 0.454799 | 1.4:+88601715 | None:intergenic |
| GGTCATGTGGGATGTCATTT+CGG | 0.454813 | 1.4:+88607198 | None:intergenic |
| ATAGGCATGCTGAAATTTCA+AGG | 0.457266 | 1.4:-88607570 | MS.gene90096:CDS |
| GAAGTCTGTGTCTCAGCATC+AGG | 0.457590 | 1.4:+88600884 | None:intergenic |
| AATGTTTCACTATATTCTCC+AGG | 0.458569 | 1.4:+88606949 | None:intergenic |
| TCTGTAGCAACCAAGTCTTC+AGG | 0.459388 | 1.4:+88607000 | None:intergenic |
| TGCTGTAATCCACCGTCAAA+TGG | 0.461217 | 1.4:+88599671 | None:intergenic |
| TCAGGGAAGAAATCAATTCC+TGG | 0.463881 | 1.4:+88600208 | None:intergenic |
| ACAATCAATCGTGCATTGCT+TGG | 0.463882 | 1.4:+88600144 | None:intergenic |
| GTACGCTTGGTTCTCAAGGT+TGG | 0.464072 | 1.4:-88603085 | MS.gene90096:CDS |
| CTGGAGGGTGGTGAACTTGA+TGG | 0.465243 | 1.4:-88600246 | MS.gene90096:CDS |
| TCTGGTCATGGTCATGGTCG+TGG | 0.465639 | 1.4:+88609596 | None:intergenic |
| AGAAATAAGACTTGCCTGCC+GGG | 0.466275 | 1.4:+88602100 | None:intergenic |
| GCTCCTCTTTCAGATGCAGC+AGG | 0.466346 | 1.4:-88609532 | MS.gene90096:CDS |
| CAAGGGAACATGGGTGGTCC+AGG | 0.466515 | 1.4:+88609852 | None:intergenic |
| GTCAACGCCTTTGTGCAGTT+GGG | 0.466881 | 1.4:-88599606 | MS.gene90096:CDS |
| GCCTCTCAAGTTTGTTCAAG+GGG | 0.467475 | 1.4:-88609395 | MS.gene90096:CDS |
| TTTGAGAGATATTATTGTAA+AGG | 0.474214 | 1.4:-88604870 | MS.gene90096:CDS |
| CCCTTGAATTGGACACCAAA+TGG | 0.474571 | 1.4:-88609835 | MS.gene90096:CDS |
| AAGACTTCTTTAATGCTGGC+AGG | 0.474634 | 1.4:-88606891 | MS.gene90096:intron |
| AATGTTCCTAAAACAGCTCC+TGG | 0.476666 | 1.4:+88603050 | None:intergenic |
| CAATGATGAATTTGAATTCA+AGG | 0.476919 | 1.4:+88609776 | None:intergenic |
| CTTCTCTAGCTCCAATTCCA+TGG | 0.477253 | 1.4:+88600323 | None:intergenic |
| CCCATGAAACACAAAGGACT+TGG | 0.478253 | 1.4:-88609441 | MS.gene90096:CDS |
| TATTATTCTGGTCATGGTCA+TGG | 0.479350 | 1.4:+88609590 | None:intergenic |
| GTTCGGATGCCCACAGGATG+TGG | 0.479660 | 1.4:-88599566 | MS.gene90096:CDS |
| GAATTGTTATCCTGGTCAGT+TGG | 0.482327 | 1.4:+88599868 | None:intergenic |
| CCCAGTGTAAAGTGCCATCC+TGG | 0.482451 | 1.4:+88609749 | None:intergenic |
| AAGGAAGTAACTCCATAGTC+TGG | 0.482731 | 1.4:+88609650 | None:intergenic |
| GTATTAAACTTGCCCAATGC+TGG | 0.484652 | 1.4:-88609709 | MS.gene90096:CDS |
| TCTTGGATCAACCAAACAGT+TGG | 0.488116 | 1.4:-88609878 | MS.gene90096:CDS |
| GATCATTATTATTCTGGTCA+TGG | 0.489429 | 1.4:+88609584 | None:intergenic |
| ACCAGGATGGCACTTTACAC+TGG | 0.489768 | 1.4:-88609750 | MS.gene90096:CDS |
| GTCTGAAAATGACCAGCATT+GGG | 0.490068 | 1.4:+88609697 | None:intergenic |
| CCTTGCCACTCTCCTACAAA+AGG | 0.490300 | 1.4:+88609502 | None:intergenic |
| CGCAAGGAATTGGTGGAGAA+AGG | 0.490585 | 1.4:-88609365 | MS.gene90096:intron |
| TGCAATAGCTATGCGCCAAA+AGG | 0.491670 | 1.4:-88604810 | MS.gene90096:intron |
| GAATTGGACACCAAATGGTT+GGG | 0.493628 | 1.4:-88609830 | MS.gene90096:CDS |
| GCCTCAGAAGGAAGCTTCAA+TGG | 0.494456 | 1.4:-88599950 | MS.gene90096:CDS |
| CAAATTCATCATTGTCCACC+AGG | 0.495495 | 1.4:-88609767 | MS.gene90096:CDS |
| CTCATCTAGGTGTTAGGGCC+CGG | 0.496779 | 1.4:-88602118 | MS.gene90096:CDS |
| TGCCTCTCAAGTTTGTTCAA+GGG | 0.498175 | 1.4:-88609396 | MS.gene90096:CDS |
| CTTGGATCAACCAAACAGTT+GGG | 0.498856 | 1.4:-88609877 | MS.gene90096:CDS |
| AAGTTATGGGAGGTGCTAGC+TGG | 0.498916 | 1.4:-88604170 | MS.gene90096:CDS |
| GGTCTGAAAATGACCAGCAT+TGG | 0.500178 | 1.4:+88609696 | None:intergenic |
| TGCAGGGCCTGCGGATGGGG+AGG | 0.500294 | 1.4:-88599698 | MS.gene90096:CDS |
| CTGGTGGACTCCAATAATAA+GGG | 0.500895 | 1.4:+88604092 | None:intergenic |
| TGCAGTACAAATGATAGATC+TGG | 0.502410 | 1.4:+88599901 | None:intergenic |
| AGAATATAGTGAAACATTTG+TGG | 0.502804 | 1.4:-88606944 | MS.gene90096:CDS |
| AGTGGCGCCTCTGTGAGATT+GGG | 0.503050 | 1.4:-88604701 | MS.gene90096:CDS |
| TTGGCTGGAATGTCAGCAGC+TGG | 0.503812 | 1.4:-88600096 | MS.gene90096:CDS |
| GCCAATGTGGAGGACTTGGC+TGG | 0.503814 | 1.4:-88600111 | MS.gene90096:CDS |
| GCCATTGAAGCTTCCTTCTG+AGG | 0.504435 | 1.4:+88599949 | None:intergenic |
| GTGTCCAATTCAAGGGAACA+TGG | 0.504504 | 1.4:+88609842 | None:intergenic |
| TTTGTGTTTCATGGGTCTGA+TGG | 0.505071 | 1.4:+88609449 | None:intergenic |
| CTTGGTGAAACTCTAGCTTC+AGG | 0.505406 | 1.4:-88599820 | MS.gene90096:CDS |
| ACAGTCTTTGCAGATGCCAT+TGG | 0.505593 | 1.4:-88600033 | MS.gene90096:CDS |
| TGGTCTATGCCACCAACTCC+AGG | 0.506227 | 1.4:-88600226 | MS.gene90096:CDS |
| TGTACAGTGAAGCCCATACT+TGG | 0.510689 | 1.4:+88600010 | None:intergenic |
| GCAAAGACTGTTGGATTGGA+AGG | 0.511836 | 1.4:+88600045 | None:intergenic |
| TCTAGGTGTTAGGGCCCGGC+AGG | 0.511895 | 1.4:-88602114 | MS.gene90096:intron |
| AAGTCGCATACCCAACCATT+TGG | 0.512231 | 1.4:+88609820 | None:intergenic |
| TATCATGTGGGAAATTTGAT+GGG | 0.512524 | 1.4:-88599774 | MS.gene90096:CDS |
| CAAAGCATTCAAAGACTCCA+TGG | 0.513340 | 1.4:+88604889 | None:intergenic |
| GGATTGGAAGGACTCACATT+GGG | 0.513845 | 1.4:+88600057 | None:intergenic |
| TGAATTGGACACCAAATGGT+TGG | 0.514554 | 1.4:-88609831 | MS.gene90096:CDS |
| AAATCAATTCCTGGAGTTGG+TGG | 0.514647 | 1.4:+88600217 | None:intergenic |
| AGCTGCTGGTGTGCCTCAGA+AGG | 0.514713 | 1.4:-88599962 | MS.gene90096:CDS |
| TTAGATCACCAAGTTCAATT+CGG | 0.515191 | 1.4:-88609916 | MS.gene90096:CDS |
| TTCATGGGTCTGATGGTCGT+TGG | 0.517749 | 1.4:+88609456 | None:intergenic |
| TAGAATCTCCAAATATAGTC+AGG | 0.519197 | 1.4:-88601637 | MS.gene90096:intron |
| ATGGCATCTGCAAAGACTGT+TGG | 0.520181 | 1.4:+88600036 | None:intergenic |
| CAAGTTGTCGACGGAAAACT+GGG | 0.521663 | 1.4:+88599629 | None:intergenic |
| AAACTCTAGCTTCAGGAACC+CGG | 0.522157 | 1.4:-88599813 | MS.gene90096:CDS |
| CAGATACTAGGAAAAGCTAT+AGG | 0.523724 | 1.4:-88603387 | MS.gene90096:CDS |
| TTGCCTCACTTATCTCATCT+AGG | 0.523928 | 1.4:-88602131 | MS.gene90096:CDS |
| CAAGTCCTCCACATTGGCGC+TGG | 0.523932 | 1.4:+88600116 | None:intergenic |
| ACAGAAGCGGAGATTCGTGC+TGG | 0.524256 | 1.4:-88603336 | MS.gene90096:CDS |
| GGTGCAAAGGCGGCTGCATG+TGG | 0.525436 | 1.4:-88600860 | MS.gene90096:CDS |
| CCTCCACAGAATTGTTATCC+TGG | 0.525913 | 1.4:+88599860 | None:intergenic |
| ATGACAGTTTACAGCGATCA+GGG | 0.526473 | 1.4:-88600396 | MS.gene90096:intron |
| TACTATGAGCCCAACTGACC+AGG | 0.526612 | 1.4:-88599878 | MS.gene90096:CDS |
| ACATGCAGCCGCCTTTGCAC+CGG | 0.527139 | 1.4:+88600862 | None:intergenic |
| AGTCTTCAGGACCAGCGTTA+CGG | 0.527185 | 1.4:+88607013 | None:intergenic |
| GACCAGAATAATAATGATCA+GGG | 0.527886 | 1.4:-88609580 | MS.gene90096:CDS |
| GAAGGAACCTGAAAGGAAGC+AGG | 0.528930 | 1.4:+88600369 | None:intergenic |
| CCTCTGTGAGATTGGGCTTG+AGG | 0.529091 | 1.4:-88604694 | MS.gene90096:CDS |
| AAAGACATCATTGAAAACAT+TGG | 0.531001 | 1.4:-88600177 | MS.gene90096:CDS |
| GCCGGCAGGTAACAGCTGCT+GGG | 0.531899 | 1.4:-88602187 | MS.gene90096:intron |
| GATGTGGAAGGATGTCTTGT+TGG | 0.534790 | 1.4:-88599550 | MS.gene90096:CDS |
| AGTTAGATAATTTCATCTGG+TGG | 0.534870 | 1.4:+88604076 | None:intergenic |
| AAGAAATCAATTCCTGGAGT+TGG | 0.535317 | 1.4:+88600214 | None:intergenic |
| TTCCTTTCAGGTTCCTTCTG+GGG | 0.537441 | 1.4:-88600364 | MS.gene90096:CDS |
| GCACTTCTCCAGAATATGGA+TGG | 0.538299 | 1.4:-88604965 | MS.gene90096:intron |
| GCCTTGAATGAAGGCAAACA+TGG | 0.538777 | 1.4:+88607269 | None:intergenic |
| TCCAATTCCATGGATCCAAA+TGG | 0.538812 | 1.4:+88600333 | None:intergenic |
| TGTTGTTCATTGAAATTCAA+AGG | 0.538965 | 1.4:+88609631 | None:intergenic |
| ATTGTAAAGGGTCTTGAAAG+TGG | 0.539311 | 1.4:-88604857 | MS.gene90096:CDS |
| AGTTGCTTGTACGCGGTGCA+GGG | 0.540037 | 1.4:-88599714 | MS.gene90096:CDS |
| ATCTGCAAAGACTGTTGGAT+TGG | 0.540161 | 1.4:+88600041 | None:intergenic |
| AGAAGTACGCTTGGTTCTCA+AGG | 0.540545 | 1.4:-88603089 | MS.gene90096:CDS |
| ATCAAAGCGCAAGGAATTGG+TGG | 0.540940 | 1.4:-88609372 | MS.gene90096:CDS |
| AGGTTGGAAGCATCTGCCGC+TGG | 0.541808 | 1.4:-88601768 | MS.gene90096:CDS |
| ATAGGACCTTCCACTGGAGA+TGG | 0.542144 | 1.4:+88602314 | None:intergenic |
| GCAAGAAGATTTGGACATTG+AGG | 0.545578 | 1.4:-88603861 | MS.gene90096:CDS |
| TAACGCTGGTCCTGAAGACT+TGG | 0.545660 | 1.4:-88607010 | MS.gene90096:CDS |
| AGATGCCATTGGCCAAGTAT+GGG | 0.546034 | 1.4:-88600022 | MS.gene90096:CDS |
| TTTGAATTCAAGGTGATCGC+CGG | 0.546998 | 1.4:+88609786 | None:intergenic |
| TATCCATTCATATTTCTACT+GGG | 0.548101 | 1.4:+88599492 | None:intergenic |
| GGCCCTGCTGCATCTGAAAG+AGG | 0.549272 | 1.4:+88609529 | None:intergenic |
| TGGGTTGTTACCATCTCCAG+TGG | 0.549865 | 1.4:-88602324 | MS.gene90096:CDS |
| TGGATGACCTCCCCATCCGC+AGG | 0.551136 | 1.4:+88599691 | None:intergenic |
| CCATTTGGTGTCCAATTCAA+GGG | 0.551707 | 1.4:+88609835 | None:intergenic |
| ATATAGTCGGAAGTGTTCAT+GGG | 0.552947 | 1.4:-88607785 | MS.gene90096:CDS |
| AGCTGCTGGGAGGAACATAG+TGG | 0.553213 | 1.4:-88602174 | MS.gene90096:CDS |
| TGGGGAGGTCATCCATTTGA+CGG | 0.553323 | 1.4:-88599683 | MS.gene90096:CDS |
| CAAAGCTGACGGTGATGAAG+AGG | 0.560582 | 1.4:-88602276 | MS.gene90096:intron |
| AAAATAACCTTGAGGTCATG+TGG | 0.562951 | 1.4:+88607185 | None:intergenic |
| AACTCTAGCTTCAGGAACCC+GGG | 0.563497 | 1.4:-88599812 | MS.gene90096:CDS |
| GTGAATTCTGCCTTGAATGA+AGG | 0.564824 | 1.4:+88607260 | None:intergenic |
| TCGAAAGGATATATCTCCAC+AGG | 0.564996 | 1.4:-88607515 | MS.gene90096:intron |
| TGATGGGCTTGTGCAAACAC+TGG | 0.566697 | 1.4:-88599758 | MS.gene90096:CDS |
| CCAGGATGGCACTTTACACT+GGG | 0.568160 | 1.4:-88609749 | MS.gene90096:CDS |
| AGGGTCTTGAAAGTGGCCTC+AGG | 0.569043 | 1.4:-88604850 | MS.gene90096:CDS |
| TTGTTACCATCTCCAGTGGA+AGG | 0.569816 | 1.4:-88602320 | MS.gene90096:CDS |
| TTGAAAATGAGCTTATTGCA+TGG | 0.570806 | 1.4:-88604029 | MS.gene90096:CDS |
| GGCTTCACTGTACACGCGAA+GGG | 0.571618 | 1.4:-88600001 | MS.gene90096:CDS |
| GAGAAAGTTCGGATGCCCAC+AGG | 0.572628 | 1.4:-88599572 | MS.gene90096:CDS |
| ATACTCTTCTATCTATCTGA+AGG | 0.573630 | 1.4:-88607739 | MS.gene90096:intron |
| ATTCTACCAGCAGAGAAAGC+AGG | 0.577027 | 1.4:+88601654 | None:intergenic |
| AGGTTGAAAGAATTGTTCCT+GGG | 0.577202 | 1.4:-88602343 | MS.gene90096:CDS |
| CTCCTCTTTCAGATGCAGCA+GGG | 0.578168 | 1.4:-88609531 | MS.gene90096:CDS |
| CTCATTGTTAACAAAGCTGA+CGG | 0.584239 | 1.4:-88602287 | MS.gene90096:CDS |
| CTTATTGCATGGAGTGCAAG+AGG | 0.585968 | 1.4:-88604018 | MS.gene90096:CDS |
| AACAAGCTTCACCGTAACGC+TGG | 0.586434 | 1.4:-88607024 | MS.gene90096:CDS |
| CGTCAGCTTTGTTAACAATG+AGG | 0.586566 | 1.4:+88602288 | None:intergenic |
| ATGTTTCACTATATTCTCCA+GGG | 0.588007 | 1.4:+88606950 | None:intergenic |
| CAAAGGACTTGGGATACCTC+AGG | 0.590864 | 1.4:-88609430 | MS.gene90096:CDS |
| AAAGCTGTGAGAAGTACGCT+TGG | 0.590866 | 1.4:-88603098 | MS.gene90096:CDS |
| GACACAGACTTCCGGTGCAA+AGG | 0.592849 | 1.4:-88600873 | MS.gene90096:CDS |
| TTCATCATTGTCCACCAGGA+TGG | 0.593734 | 1.4:-88609763 | MS.gene90096:CDS |
| TTCAAGGGGATCAAAGCGCA+AGG | 0.595435 | 1.4:-88609381 | MS.gene90096:CDS |
| GCCATCAAGGTCAACTGAAG+AGG | 0.595713 | 1.4:+88601728 | None:intergenic |
| CTTATGCAGTGGATAAACAC+AGG | 0.598140 | 1.4:-88607634 | MS.gene90096:intron |
| AGACATCCTTCCACATCCTG+TGG | 0.598150 | 1.4:+88599556 | None:intergenic |
| TGACATCCCACATGACCTCA+AGG | 0.598443 | 1.4:-88607192 | MS.gene90096:intron |
| ACAGACTTCCGGTGCAAAGG+CGG | 0.598486 | 1.4:-88600870 | MS.gene90096:CDS |
| TGCGACTTCCATTTCAACGC+CGG | 0.599134 | 1.4:-88609805 | MS.gene90096:CDS |
| TAAAGACTTGAATATTTCAG+TGG | 0.600082 | 1.4:+88600293 | None:intergenic |
| CCATGAAACACAAAGGACTT+GGG | 0.601641 | 1.4:-88609440 | MS.gene90096:CDS |
| ATCCATTCATATTTCTACTG+GGG | 0.601788 | 1.4:+88599493 | None:intergenic |
| ACACCATGGCGTATATCATG+TGG | 0.604594 | 1.4:-88599787 | MS.gene90096:CDS |
| AACTCTTGGAATGATCCACT+TGG | 0.608296 | 1.4:-88604117 | MS.gene90096:CDS |
| AGCGAGGAGTTGCTTGTACG+CGG | 0.609309 | 1.4:-88599721 | MS.gene90096:CDS |
| TATACGCCATGGTGTACCCC+GGG | 0.609937 | 1.4:+88599795 | None:intergenic |
| TTTCAGCATGCCTATTGGGA+CGG | 0.611247 | 1.4:+88607578 | None:intergenic |
| ATATACGCCATGGTGTACCC+CGG | 0.611453 | 1.4:+88599794 | None:intergenic |
| TCTGAGGCACACCAGCAGCT+CGG | 0.611849 | 1.4:+88599965 | None:intergenic |
| GGCATCCGAACTTTCTCTCG+AGG | 0.611937 | 1.4:+88599578 | None:intergenic |
| GCTAAAATCACAAAGAACCC+TGG | 0.612235 | 1.4:-88606967 | MS.gene90096:CDS |
| GGCGTTGACCAAGTTGTCGA+CGG | 0.612768 | 1.4:+88599620 | None:intergenic |
| TGGATTGGAAGGACTCACAT+TGG | 0.612854 | 1.4:+88600056 | None:intergenic |
| TCGATCCAGCGCCAATGTGG+AGG | 0.615600 | 1.4:-88600121 | MS.gene90096:CDS |
| AATGGTAAAACTGCCCCAGA+AGG | 0.615771 | 1.4:+88600351 | None:intergenic |
| TGTCAAAACATATACAGAAG+CGG | 0.618415 | 1.4:-88603349 | MS.gene90096:CDS |
| TCCAGCCAAGTCCTCCACAT+TGG | 0.618432 | 1.4:+88600110 | None:intergenic |
| GATCTTGTACGTGATATAGT+CGG | 0.620324 | 1.4:-88607798 | MS.gene90096:CDS |
| TGCCCCAGAAGGAACCTGAA+AGG | 0.621431 | 1.4:+88600362 | None:intergenic |
| AGGATAATAGGACCTTCCAC+TGG | 0.621720 | 1.4:+88602308 | None:intergenic |
| CAGCATGCCTATTGGGACGG+TGG | 0.622344 | 1.4:+88607581 | None:intergenic |
| AGAGCAACATACAAGTCGAA+AGG | 0.623148 | 1.4:-88607530 | MS.gene90096:CDS |
| GACATCCTTCCACATCCTGT+GGG | 0.623860 | 1.4:+88599557 | None:intergenic |
| TCCCCTTGAACAAACTTGAG+AGG | 0.625441 | 1.4:+88609394 | None:intergenic |
| ACATTTATATTGTTCAGACA+AGG | 0.626137 | 1.4:-88599522 | MS.gene90096:CDS |
| GGGCTTCACTGTACACGCGA+AGG | 0.626376 | 1.4:-88600002 | MS.gene90096:CDS |
| TCATGGGGAAGATCAATTGG+AGG | 0.627527 | 1.4:-88607769 | MS.gene90096:CDS |
| CAGGAACCCGGGGTACACCA+TGG | 0.628230 | 1.4:-88599801 | MS.gene90096:CDS |
| TGTACGCGGTGCAGGGCCTG+CGG | 0.628396 | 1.4:-88599707 | MS.gene90096:CDS |
| TGACCAGGATAACAATTCTG+TGG | 0.628898 | 1.4:-88599863 | MS.gene90096:CDS |
| TGTTCGATCCAGCGCCAATG+TGG | 0.629076 | 1.4:-88600124 | MS.gene90096:CDS |
| TTCCCACATGATATACGCCA+TGG | 0.630616 | 1.4:+88599784 | None:intergenic |
| GGATGCCCACAGGATGTGGA+AGG | 0.631872 | 1.4:-88599562 | MS.gene90096:CDS |
| CACTCTCCTACAAAAGGAGT+CGG | 0.633614 | 1.4:+88609508 | None:intergenic |
| TGAAGCCCATACTTGGCCAA+TGG | 0.633954 | 1.4:+88600017 | None:intergenic |
| CACCATGGCGTATATCATGT+GGG | 0.640615 | 1.4:-88599786 | MS.gene90096:CDS |
| CTACTCTTGATAGATCAAAG+AGG | 0.641081 | 1.4:-88603831 | MS.gene90096:CDS |
| CCAGAATAATAATGATCAGG+GGG | 0.641184 | 1.4:-88609578 | MS.gene90096:CDS |
| AAATAACCTTGAGGTCATGT+GGG | 0.642385 | 1.4:+88607186 | None:intergenic |
| CCAGGATAACAATTCTGTGG+AGG | 0.643821 | 1.4:-88599860 | MS.gene90096:CDS |
| CCAATTCAAGGGAACATGGG+TGG | 0.646472 | 1.4:+88609846 | None:intergenic |
| TCAACAGCTTCAAAAGAACA+AGG | 0.648721 | 1.4:-88604932 | MS.gene90096:CDS |
| GACAGTTTACAGCGATCAGG+GGG | 0.651901 | 1.4:-88600394 | MS.gene90096:intron |
| CAGAAGCGGAGATTCGTGCT+GGG | 0.654251 | 1.4:-88603335 | MS.gene90096:intron |
| TGACCTCCATCTTCAAAACA+TGG | 0.654311 | 1.4:+88607604 | None:intergenic |
| AGAGGCATTTCAGAAAGCGA+AGG | 0.656834 | 1.4:-88604000 | MS.gene90096:intron |
| ACCAGAATAATAATGATCAG+GGG | 0.660108 | 1.4:-88609579 | MS.gene90096:CDS |
| TTGATCCAAGAAGCACAACA+TGG | 0.661070 | 1.4:+88609890 | None:intergenic |
| AGTGTAAAGTGCCATCCTGG+TGG | 0.661255 | 1.4:+88609752 | None:intergenic |
| TGACAGTTTACAGCGATCAG+GGG | 0.663002 | 1.4:-88600395 | MS.gene90096:intron |
| GATAGAAACAGCAAAACTGG+AGG | 0.665406 | 1.4:-88600262 | MS.gene90096:CDS |
| AAAACTGGGTCAACTGACAG+TGG | 0.666516 | 1.4:+88599643 | None:intergenic |
| TGTCCAATTCAAGGGAACAT+GGG | 0.666516 | 1.4:+88609843 | None:intergenic |
| GCTGCTGGGAGGAACATAGT+GGG | 0.668743 | 1.4:-88602173 | MS.gene90096:CDS |
| AACTGGAAACCAGAAGAATG+TGG | 0.670332 | 1.4:-88604057 | MS.gene90096:CDS |
| ACTGGAGATGGTAACAACCC+AGG | 0.673226 | 1.4:+88602326 | None:intergenic |
| AGGCAAACCTTGAAGACCTG+AGG | 0.673854 | 1.4:+88609414 | None:intergenic |
| GGCAGGTAACAGCTGCTGGG+AGG | 0.674086 | 1.4:-88602184 | MS.gene90096:intron |
| ATAGAAACAGCAAAACTGGA+GGG | 0.675152 | 1.4:-88600261 | MS.gene90096:CDS |
| ATCAGACCCATGAAACACAA+AGG | 0.676250 | 1.4:-88609447 | MS.gene90096:CDS |
| ACTCTAGCTTCAGGAACCCG+GGG | 0.679858 | 1.4:-88599811 | MS.gene90096:CDS |
| GAGGCACACCAGCAGCTCGG+CGG | 0.681557 | 1.4:+88599968 | None:intergenic |
| GGAGGTCATCCATTTGACGG+TGG | 0.690604 | 1.4:-88599680 | MS.gene90096:CDS |
| AACGTGCTTGAAGTTATGGG+AGG | 0.692858 | 1.4:-88604180 | MS.gene90096:CDS |
| AATCTGGAGCATCATTCCTG+AGG | 0.696097 | 1.4:+88604834 | None:intergenic |
| CCAATAATAAGGGCTCCAAG+TGG | 0.696784 | 1.4:+88604102 | None:intergenic |
| TAGATCAAAGAGGCTTACTG+AGG | 0.699723 | 1.4:-88603821 | MS.gene90096:CDS |
| ACACCTAGATGAGATAAGTG+AGG | 0.701643 | 1.4:+88602128 | None:intergenic |
| TTATGCAGTGGATAAACACA+GGG | 0.702208 | 1.4:-88607633 | MS.gene90096:intron |
| GAAACAGCAAAACTGGAGGG+TGG | 0.706976 | 1.4:-88600258 | MS.gene90096:CDS |
| TATAGTCGGAAGTGTTCATG+GGG | 0.710598 | 1.4:-88607784 | MS.gene90096:CDS |
| CTGAAGCTAGAGTTTCACCA+AGG | 0.724048 | 1.4:+88599821 | None:intergenic |
| CAACGTCAGATTAACACCAG+CGG | 0.726780 | 1.4:+88601752 | None:intergenic |
| CCTCAAGCCCAATCTCACAG+AGG | 0.778276 | 1.4:+88604694 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 88599507 | 88610001 | 88599507 | ID=MS.gene90096 |
| chr1.4 | mRNA | 88599507 | 88610001 | 88599507 | ID=MS.gene90096.t1;Parent=MS.gene90096 |
| chr1.4 | exon | 88609366 | 88610001 | 88609366 | ID=MS.gene90096.t1.exon1;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88609366 | 88610001 | 88609366 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88607740 | 88607823 | 88607740 | ID=MS.gene90096.t1.exon2;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88607740 | 88607823 | 88607740 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88607516 | 88607647 | 88607516 | ID=MS.gene90096.t1.exon3;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88607516 | 88607647 | 88607516 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88607193 | 88607318 | 88607193 | ID=MS.gene90096.t1.exon4;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88607193 | 88607318 | 88607193 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88606892 | 88607070 | 88606892 | ID=MS.gene90096.t1.exon5;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88606892 | 88607070 | 88606892 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88605322 | 88605406 | 88605322 | ID=MS.gene90096.t1.exon6;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88605322 | 88605406 | 88605322 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88604811 | 88604975 | 88604811 | ID=MS.gene90096.t1.exon7;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88604811 | 88604975 | 88604811 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88604669 | 88604721 | 88604669 | ID=MS.gene90096.t1.exon8;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88604669 | 88604721 | 88604669 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88604001 | 88604209 | 88604001 | ID=MS.gene90096.t1.exon9;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88604001 | 88604209 | 88604001 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88603777 | 88603895 | 88603777 | ID=MS.gene90096.t1.exon10;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88603777 | 88603895 | 88603777 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88603336 | 88603406 | 88603336 | ID=MS.gene90096.t1.exon11;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88603336 | 88603406 | 88603336 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88603043 | 88603160 | 88603043 | ID=MS.gene90096.t1.exon12;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88603043 | 88603160 | 88603043 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88602277 | 88602363 | 88602277 | ID=MS.gene90096.t1.exon13;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88602277 | 88602363 | 88602277 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88602115 | 88602201 | 88602115 | ID=MS.gene90096.t1.exon14;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88602115 | 88602201 | 88602115 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88601940 | 88602016 | 88601940 | ID=MS.gene90096.t1.exon15;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88601940 | 88602016 | 88601940 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88601638 | 88601788 | 88601638 | ID=MS.gene90096.t1.exon16;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88601638 | 88601788 | 88601638 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88600825 | 88600936 | 88600825 | ID=MS.gene90096.t1.exon17;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88600825 | 88600936 | 88600825 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| chr1.4 | exon | 88599507 | 88600411 | 88599507 | ID=MS.gene90096.t1.exon18;Parent=MS.gene90096.t1 |
| chr1.4 | CDS | 88599507 | 88600411 | 88599507 | ID=cds.MS.gene90096.t1;Parent=MS.gene90096.t1 |
| Gene Sequence |
| Protein sequence |
