Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060569.t1 | XP_013458057.1 | 89.2 | 260 | 3 | 1 | 1 | 235 | 1 | 260 | 6.00E-116 | 427.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060569.t1 | Q9FNN6 | 44.7 | 219 | 71 | 4 | 3 | 196 | 7 | 200 | 1.5e-30 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060569.t1 | A0A072USM0 | 89.2 | 260 | 3 | 1 | 1 | 235 | 1 | 260 | 4.3e-116 | 427.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene060569.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050432 | MS.gene060569 | 0.804168 | 2.46E-49 | -1.69E-46 |
| MS.gene051360 | MS.gene060569 | 0.840623 | 7.78E-58 | -1.69E-46 |
| MS.gene052485 | MS.gene060569 | 0.800406 | 1.46E-48 | -1.69E-46 |
| MS.gene053209 | MS.gene060569 | 0.829722 | 4.39E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene060569 | 0.803606 | 3.22E-49 | -1.69E-46 |
| MS.gene055666 | MS.gene060569 | 0.809468 | 1.86E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene060569 | 0.807805 | 4.22E-50 | -1.69E-46 |
| MS.gene05806 | MS.gene060569 | 0.801674 | 8.05E-49 | -1.69E-46 |
| MS.gene058365 | MS.gene060569 | 0.816408 | 5.62E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene060569 | 0.802281 | 6.04E-49 | -1.69E-46 |
| MS.gene059692 | MS.gene060569 | 0.813981 | 1.94E-51 | -1.69E-46 |
| MS.gene059695 | MS.gene060569 | 0.818878 | 1.56E-52 | -1.69E-46 |
| MS.gene059711 | MS.gene060569 | 0.803764 | 2.98E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene060837 | 0.807556 | 4.77E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene061470 | 0.800167 | 1.64E-48 | -1.69E-46 |
| MS.gene060569 | MS.gene062911 | 0.812977 | 3.23E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene067125 | 0.810902 | 9.15E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene067414 | 0.809269 | 2.06E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene068240 | 0.814709 | 1.34E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene069435 | 0.80351 | 3.37E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene069680 | 0.804795 | 1.82E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene069681 | 0.806954 | 6.40E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene069682 | 0.808889 | 2.48E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene070164 | 0.822127 | 2.80E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene070266 | 0.815698 | 8.09E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene07096 | 0.809869 | 1.53E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene071757 | 0.821366 | 4.19E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene071758 | 0.833522 | 5.08E-56 | -1.69E-46 |
| MS.gene060569 | MS.gene072304 | 0.802388 | 5.74E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene073295 | 0.808392 | 3.17E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene073925 | 0.830095 | 3.56E-55 | -1.69E-46 |
| MS.gene060569 | MS.gene073926 | 0.82928 | 5.62E-55 | -1.69E-46 |
| MS.gene060569 | MS.gene07619 | 0.801119 | 1.05E-48 | -1.69E-46 |
| MS.gene060569 | MS.gene09574 | 0.80052 | 1.39E-48 | -1.69E-46 |
| MS.gene060569 | MS.gene20264 | 0.823498 | 1.34E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene20275 | 0.811223 | 7.79E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene21005 | 0.838673 | 2.50E-57 | -1.69E-46 |
| MS.gene060569 | MS.gene21037 | 0.857076 | 2.09E-62 | -1.69E-46 |
| MS.gene060569 | MS.gene21413 | 0.85855 | 7.63E-63 | -1.69E-46 |
| MS.gene060569 | MS.gene22292 | 0.813139 | 2.98E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene23318 | 0.80618 | 9.32E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene23338 | 0.832967 | 6.99E-56 | -1.69E-46 |
| MS.gene060569 | MS.gene23621 | 0.827094 | 1.89E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene23787 | 0.827691 | 1.36E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene25354 | 0.857167 | 1.96E-62 | -1.69E-46 |
| MS.gene060569 | MS.gene25859 | 0.813673 | 2.27E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene26193 | 0.80153 | 8.62E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene27100 | 0.811457 | 6.94E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene31046 | 0.807491 | 4.93E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene31462 | 0.826258 | 2.99E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene32274 | 0.810065 | 1.39E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene34800 | 0.808501 | 3.00E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene36507 | 0.804681 | 1.92E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene39360 | 0.838165 | 3.38E-57 | -1.69E-46 |
| MS.gene060569 | MS.gene39805 | 0.813111 | 3.02E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene39912 | 0.817372 | 3.41E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene40212 | 0.82449 | 7.85E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene40773 | 0.864943 | 8.45E-65 | -1.69E-46 |
| MS.gene060569 | MS.gene41062 | 0.810096 | 1.37E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene43587 | 0.805471 | 1.31E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene43588 | 0.80497 | 1.67E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene43589 | 0.804091 | 2.55E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene43782 | 0.804547 | 2.05E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene44558 | 0.820927 | 5.30E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene48593 | 0.802114 | 6.54E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene49350 | 0.819301 | 1.25E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene49722 | 0.805093 | 1.58E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene50575 | 0.819275 | 1.27E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene50721 | 0.869925 | 2.15E-66 | -1.69E-46 |
| MS.gene060569 | MS.gene53276 | 0.840977 | 6.28E-58 | -1.69E-46 |
| MS.gene060569 | MS.gene53309 | 0.804069 | 2.58E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene53311 | 0.808419 | 3.13E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene53556 | 0.822933 | 1.82E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene55342 | 0.808354 | 3.23E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene56204 | 0.811336 | 7.37E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene58120 | 0.815401 | 9.42E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene58499 | 0.80242 | 5.66E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene58537 | 0.820182 | 7.85E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene58804 | 0.819649 | 1.04E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene60238 | 0.808391 | 3.17E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene60558 | 0.80572 | 1.16E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene61160 | 0.808428 | 3.11E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene62087 | 0.826732 | 2.31E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene63115 | 0.80548 | 1.31E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene64994 | 0.8193 | 1.25E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene65588 | 0.810374 | 1.19E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene66800 | 0.80212 | 6.52E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene68122 | 0.80114 | 1.04E-48 | -1.69E-46 |
| MS.gene060569 | MS.gene68423 | 0.861922 | 7.30E-64 | -1.69E-46 |
| MS.gene060569 | MS.gene68963 | 0.824141 | 9.48E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene68967 | 0.805742 | 1.15E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene68968 | 0.804364 | 2.24E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene70101 | 0.801581 | 8.42E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene70267 | 0.8184 | 2.00E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene70333 | 0.800008 | 1.76E-48 | -1.69E-46 |
| MS.gene060569 | MS.gene70352 | 0.817355 | 3.44E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene71024 | 0.830159 | 3.44E-55 | -1.69E-46 |
| MS.gene060569 | MS.gene71664 | 0.833745 | 4.47E-56 | -1.69E-46 |
| MS.gene060569 | MS.gene71665 | 0.802306 | 5.97E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene71667 | 0.819296 | 1.25E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene71861 | 0.803844 | 2.87E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene72858 | 0.820051 | 8.41E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene74261 | 0.842514 | 2.47E-58 | -1.69E-46 |
| MS.gene060569 | MS.gene75493 | 0.815913 | 7.24E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene77809 | 0.820537 | 6.51E-53 | -1.69E-46 |
| MS.gene060569 | MS.gene78852 | 0.825847 | 3.75E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene79722 | 0.805924 | 1.06E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene79762 | 0.810747 | 9.89E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene80413 | 0.815164 | 1.06E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene82534 | 0.82408 | 9.80E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene84154 | 0.802276 | 6.06E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene86413 | 0.810733 | 9.95E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene87850 | 0.818725 | 1.69E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene88520 | 0.801641 | 8.18E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene88667 | 0.803403 | 3.54E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene88768 | 0.805999 | 1.02E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene90096 | 0.825891 | 3.66E-54 | -1.69E-46 |
| MS.gene060569 | MS.gene90907 | 0.832598 | 8.63E-56 | -1.69E-46 |
| MS.gene060569 | MS.gene94333 | 0.80652 | 7.91E-50 | -1.69E-46 |
| MS.gene060569 | MS.gene96206 | 0.815643 | 8.32E-52 | -1.69E-46 |
| MS.gene060569 | MS.gene97081 | 0.804358 | 2.24E-49 | -1.69E-46 |
| MS.gene060569 | MS.gene97224 | 0.800231 | 1.59E-48 | -1.69E-46 |
| MS.gene060569 | MS.gene99375 | 0.81102 | 8.63E-51 | -1.69E-46 |
| MS.gene060569 | MS.gene99622 | 0.806883 | 6.62E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene060569 | MS.gene44859 | PPI |
| MS.gene060569 | MS.gene04050 | PPI |
| MS.gene060569 | MS.gene43756 | PPI |
| MS.gene060569 | MS.gene04461 | PPI |
| MS.gene060569 | MS.gene88743 | PPI |
| MS.gene060569 | MS.gene05604 | PPI |
| MS.gene060569 | MS.gene009920 | PPI |
| MS.gene060569 | MS.gene04574 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060569.t1 | MTR_4g111975 | 90.429 | 303 | 4 | 1 | 1 | 278 | 1 | 303 | 0.0 | 553 |
| MS.gene060569.t1 | MTR_8g063600 | 38.889 | 288 | 88 | 13 | 8 | 253 | 10 | 251 | 6.70e-47 | 159 |
| MS.gene060569.t1 | MTR_3g462790 | 39.234 | 209 | 66 | 6 | 8 | 186 | 43 | 220 | 2.67e-33 | 124 |
| MS.gene060569.t1 | MTR_7g067080 | 30.844 | 308 | 138 | 10 | 3 | 267 | 30 | 305 | 3.28e-33 | 123 |
| MS.gene060569.t1 | MTR_5g069710 | 38.235 | 204 | 64 | 5 | 8 | 181 | 29 | 200 | 7.53e-33 | 122 |
| MS.gene060569.t1 | MTR_1g033620 | 54.225 | 142 | 42 | 5 | 146 | 271 | 38 | 172 | 1.48e-31 | 117 |
| MS.gene060569.t1 | MTR_1g111830 | 35.500 | 200 | 74 | 4 | 8 | 182 | 24 | 193 | 1.03e-30 | 115 |
| MS.gene060569.t1 | MTR_8g063870 | 34.155 | 284 | 142 | 12 | 8 | 271 | 8 | 266 | 4.77e-30 | 114 |
| MS.gene060569.t1 | MTR_5g081860 | 32.857 | 210 | 76 | 6 | 8 | 187 | 27 | 201 | 2.13e-26 | 104 |
| MS.gene060569.t1 | MTR_1g033600 | 43.885 | 139 | 58 | 4 | 8 | 146 | 12 | 130 | 4.64e-26 | 100 |
| MS.gene060569.t1 | MTR_1g022290 | 46.667 | 75 | 36 | 2 | 1 | 75 | 1 | 71 | 1.06e-16 | 73.9 |
| MS.gene060569.t1 | MTR_3g111880 | 46.667 | 75 | 37 | 2 | 3 | 77 | 2 | 73 | 2.83e-16 | 72.8 |
| MS.gene060569.t1 | MTR_6g092540 | 48.611 | 72 | 12 | 1 | 132 | 178 | 91 | 162 | 1.70e-14 | 72.4 |
| MS.gene060569.t1 | MTR_6g004250 | 45.946 | 74 | 37 | 1 | 2 | 75 | 4 | 74 | 2.32e-14 | 67.4 |
| MS.gene060569.t1 | MTR_4g100630 | 44.706 | 85 | 22 | 1 | 127 | 186 | 84 | 168 | 1.62e-13 | 69.7 |
| MS.gene060569.t1 | MTR_8g101650 | 47.887 | 71 | 11 | 2 | 132 | 177 | 99 | 168 | 6.43e-13 | 67.8 |
| MS.gene060569.t1 | MTR_3g116720 | 41.176 | 68 | 37 | 1 | 6 | 73 | 14 | 78 | 7.07e-13 | 63.2 |
| MS.gene060569.t1 | MTR_2g100930 | 40.426 | 94 | 24 | 2 | 109 | 177 | 87 | 173 | 1.33e-11 | 64.3 |
| MS.gene060569.t1 | MTR_3g013440 | 43.421 | 76 | 38 | 3 | 1 | 75 | 4 | 75 | 8.65e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060569.t1 | AT1G49010 | 46.866 | 335 | 99 | 12 | 1 | 277 | 1 | 314 | 2.08e-68 | 214 |
| MS.gene060569.t1 | AT5G08520 | 43.493 | 292 | 92 | 10 | 3 | 258 | 7 | 261 | 1.62e-55 | 181 |
| MS.gene060569.t1 | AT2G38090 | 36.634 | 202 | 85 | 3 | 5 | 181 | 26 | 209 | 3.68e-36 | 131 |
| MS.gene060569.t1 | AT5G01200 | 38.235 | 204 | 81 | 4 | 6 | 181 | 28 | 214 | 6.82e-33 | 121 |
| MS.gene060569.t1 | AT5G23650 | 37.054 | 224 | 88 | 6 | 3 | 199 | 8 | 205 | 2.42e-32 | 122 |
| MS.gene060569.t1 | AT5G58900 | 37.799 | 209 | 74 | 7 | 2 | 181 | 28 | 209 | 7.51e-32 | 119 |
| MS.gene060569.t1 | AT5G05790 | 30.741 | 270 | 116 | 9 | 1 | 229 | 24 | 263 | 1.29e-27 | 108 |
| MS.gene060569.t1 | AT5G05790 | 30.741 | 270 | 116 | 9 | 1 | 229 | 24 | 263 | 1.29e-27 | 108 |
| MS.gene060569.t1 | AT5G04760 | 33.790 | 219 | 83 | 5 | 8 | 201 | 6 | 187 | 9.74e-27 | 104 |
| MS.gene060569.t1 | AT3G11280 | 35.176 | 199 | 68 | 4 | 8 | 181 | 33 | 195 | 2.11e-26 | 104 |
| MS.gene060569.t1 | AT3G11280 | 35.176 | 199 | 68 | 4 | 8 | 181 | 33 | 195 | 2.11e-26 | 104 |
| MS.gene060569.t1 | AT5G47390 | 41.284 | 109 | 36 | 2 | 112 | 192 | 67 | 175 | 1.19e-14 | 73.6 |
| MS.gene060569.t1 | AT3G10580 | 31.959 | 194 | 108 | 8 | 1 | 194 | 1 | 170 | 1.42e-14 | 72.0 |
| MS.gene060569.t1 | AT3G10580 | 31.959 | 194 | 108 | 8 | 1 | 194 | 1 | 170 | 1.64e-14 | 72.4 |
| MS.gene060569.t1 | AT4G39250 | 40.909 | 66 | 36 | 1 | 8 | 73 | 14 | 76 | 3.36e-13 | 64.7 |
| MS.gene060569.t1 | AT5G61620 | 30.612 | 196 | 79 | 8 | 112 | 273 | 84 | 256 | 5.92e-13 | 68.2 |
| MS.gene060569.t1 | AT1G75250 | 39.437 | 71 | 40 | 1 | 3 | 73 | 7 | 74 | 1.36e-12 | 63.2 |
| MS.gene060569.t1 | AT1G75250 | 39.394 | 66 | 37 | 1 | 8 | 73 | 12 | 74 | 3.87e-12 | 62.8 |
Find 67 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGATCAAGTTGTAATTT+TGG | 0.168041 | 8.4:-15906737 | MS.gene060569:CDS |
| TCTAATTCATTAGCATAATT+TGG | 0.179151 | 8.4:+15906812 | None:intergenic |
| CATCATCCTCCTGGTGGTTT+AGG | 0.251182 | 8.4:-15906006 | MS.gene060569:CDS |
| TTACCATGTTGACCTGTTAT+TGG | 0.282124 | 8.4:+15906087 | None:intergenic |
| TTCAAACAAAGATTCTTCTA+AGG | 0.298166 | 8.4:-15906777 | MS.gene060569:CDS |
| CCTTCAAATACAATGGGTTT+AGG | 0.313838 | 8.4:-15906057 | MS.gene060569:CDS |
| ATCTGTCCCCAACAAAAGTA+TGG | 0.337048 | 8.4:-15906906 | MS.gene060569:CDS |
| TGTTGCTCCTCCTGCTCATA+TGG | 0.385813 | 8.4:-15905953 | MS.gene060569:CDS |
| AATCATCAAGCACCAATAAC+AGG | 0.394644 | 8.4:-15906099 | MS.gene060569:CDS |
| CCTAAACCCATTGTATTTGA+AGG | 0.400664 | 8.4:+15906057 | None:intergenic |
| ATCCACCTTTACCACTACTA+TGG | 0.406819 | 8.4:+15906678 | None:intergenic |
| GGATGATGGGGAAGCATAAC+AGG | 0.408632 | 8.4:+15905913 | None:intergenic |
| TTCTTCTTCATAGCTCCAAA+TGG | 0.430907 | 8.4:+15907006 | None:intergenic |
| CCTGGTGGTTTAGGAATGTA+TGG | 0.438589 | 8.4:-15905997 | MS.gene060569:CDS |
| TGTCATCAAGTGGAGCCATT+TGG | 0.441940 | 8.4:-15907021 | MS.gene060569:CDS |
| GCTCATATGGCATCTGCAGT+TGG | 0.444181 | 8.4:-15905940 | MS.gene060569:CDS |
| AGTTGTCATTGGTGCATTGT+TGG | 0.444498 | 8.4:+15905841 | None:intergenic |
| ATGCACAAAAGTATTTCATA+AGG | 0.449793 | 8.4:-15906200 | MS.gene060569:CDS |
| AGAAGATTCAAAAGAACAAT+GGG | 0.451020 | 8.4:-15906942 | MS.gene060569:CDS |
| CTATGCTCTTCTTCTGTCCA+TGG | 0.458260 | 8.4:+15906608 | None:intergenic |
| CTCATATGGCATCTGCAGTT+GGG | 0.466530 | 8.4:-15905939 | MS.gene060569:CDS |
| GCATGACTTGCTACTTGTGT+TGG | 0.467928 | 8.4:+15906219 | None:intergenic |
| ACAATCCCTTCAAATACAAT+GGG | 0.480739 | 8.4:-15906063 | MS.gene060569:CDS |
| CACAATCCCTTCAAATACAA+TGG | 0.482952 | 8.4:-15906064 | MS.gene060569:CDS |
| TCAGAACAAGAAAGGAGAAA+AGG | 0.492641 | 8.4:-15906635 | MS.gene060569:CDS |
| GCAACAACAGGATGTCCAAC+TGG | 0.493168 | 8.4:+15905970 | None:intergenic |
| TGTTAACACTAGTAATATCA+TGG | 0.493710 | 8.4:+15906139 | None:intergenic |
| TGAGCAGGAGGAGCAACAAC+AGG | 0.495632 | 8.4:+15905958 | None:intergenic |
| CTAAACCCATTGTATTTGAA+GGG | 0.499951 | 8.4:+15906058 | None:intergenic |
| GTGGATGATGATGAGGATGA+TGG | 0.500151 | 8.4:+15905899 | None:intergenic |
| CATCATCATCAGAACAAGAA+AGG | 0.501232 | 8.4:-15906643 | MS.gene060569:CDS |
| GTTCAGCCTCATCATCCTCC+TGG | 0.503699 | 8.4:-15906015 | MS.gene060569:CDS |
| TGGATGATGATGAGGATGAT+GGG | 0.504027 | 8.4:+15905900 | None:intergenic |
| TGTACCTCTTGCTTACCCTA+TGG | 0.504295 | 8.4:-15905869 | MS.gene060569:CDS |
| TAAGCAAGAGGTACAACATA+AGG | 0.508613 | 8.4:+15905877 | None:intergenic |
| TGCATTGTTGGAGGTGCCAT+AGG | 0.508759 | 8.4:+15905853 | None:intergenic |
| TGTCATTGGTGCATTGTTGG+AGG | 0.512443 | 8.4:+15905844 | None:intergenic |
| AGAATGCTATAGCCATGCAT+TGG | 0.517213 | 8.4:-15906973 | MS.gene060569:CDS |
| CACCTTTACCACTACTATGG+TGG | 0.519881 | 8.4:+15906681 | None:intergenic |
| ACCCACCATAGTAGTGGTAA+AGG | 0.542637 | 8.4:-15906683 | MS.gene060569:CDS |
| AAGAAGATTCAAAAGAACAA+TGG | 0.544714 | 8.4:-15906943 | MS.gene060569:CDS |
| AAAGGAGAAAAGGAATTCCA+TGG | 0.553013 | 8.4:-15906625 | MS.gene060569:CDS |
| ACTGCAGATGCCATATGAGC+AGG | 0.554621 | 8.4:+15905943 | None:intergenic |
| GGAATGTATGGAGCACCAGT+TGG | 0.557234 | 8.4:-15905985 | MS.gene060569:CDS |
| TAACATCATCTGCCAACACT+TGG | 0.564260 | 8.4:+15906864 | None:intergenic |
| CAATGAATAGAGATAGAAGA+AGG | 0.573297 | 8.4:-15906170 | MS.gene060569:CDS |
| ACCTTTACCACTACTATGGT+GGG | 0.587562 | 8.4:+15906682 | None:intergenic |
| CAGCCTCATCATCCTCCTGG+TGG | 0.590947 | 8.4:-15906012 | MS.gene060569:CDS |
| CACCATAGTAGTGGTAAAGG+TGG | 0.591929 | 8.4:-15906680 | MS.gene060569:CDS |
| AGATGATGTTAGTGCAATTG+AGG | 0.593055 | 8.4:-15906852 | MS.gene060569:CDS |
| GAAACAACATTACCAAGTGT+TGG | 0.597849 | 8.4:-15906876 | MS.gene060569:CDS |
| GGATGATGATGAGGATGATG+GGG | 0.600297 | 8.4:+15905901 | None:intergenic |
| GCAAGAGGTACAACATAAGG+TGG | 0.601447 | 8.4:+15905880 | None:intergenic |
| GATGTTAGTGCAATTGAGGA+AGG | 0.604039 | 8.4:-15906848 | MS.gene060569:CDS |
| AGGGCCAACATGTCATCAAG+TGG | 0.623051 | 8.4:-15907031 | None:intergenic |
| CCATACATTCCTAAACCACC+AGG | 0.623664 | 8.4:+15905997 | None:intergenic |
| GACTCTACCCACCATAGTAG+TGG | 0.624946 | 8.4:-15906689 | MS.gene060569:CDS |
| AAACCACCAGGAGGATGATG+AGG | 0.627717 | 8.4:+15906009 | None:intergenic |
| GTGGTAAAGGTGGATTGTCA+AGG | 0.632578 | 8.4:-15906670 | MS.gene060569:CDS |
| GCATTGTTGGAGGTGCCATA+GGG | 0.632899 | 8.4:+15905854 | None:intergenic |
| CATGGACAGAAGAAGAGCAT+AGG | 0.635255 | 8.4:-15906607 | MS.gene060569:intron |
| GATATTACTAGTGTTAACAA+TGG | 0.637555 | 8.4:-15906135 | MS.gene060569:CDS |
| GCACCAATAACAGGTCAACA+TGG | 0.640651 | 8.4:-15906090 | MS.gene060569:CDS |
| GCAGATGCCATATGAGCAGG+AGG | 0.646062 | 8.4:+15905946 | None:intergenic |
| ACATAAGGTGGATGATGATG+AGG | 0.659765 | 8.4:+15905892 | None:intergenic |
| GGTGCCATAGGGTAAGCAAG+AGG | 0.704654 | 8.4:+15905865 | None:intergenic |
| TACATTCCTAAACCACCAGG+AGG | 0.734599 | 8.4:+15906000 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGTTTCTTTTTTTTTTTC+AGG | - | chr8.4:15906548-15906567 | MS.gene060569:intron | 15.0% |
| !! | TCTAATTCATTAGCATAATT+TGG | + | chr8.4:15906059-15906078 | None:intergenic | 20.0% |
| !! | TTCAAGGTAATTAATTAACA+TGG | - | chr8.4:15906501-15906520 | MS.gene060569:intron | 20.0% |
| !!! | AAAAGATCAAGTTGTAATTT+TGG | - | chr8.4:15906131-15906150 | MS.gene060569:CDS | 20.0% |
| !!! | TTTTTCAGGTTATTTTTACT+TGG | - | chr8.4:15906562-15906581 | MS.gene060569:intron | 20.0% |
| ! | AAGAAGATTCAAAAGAACAA+TGG | - | chr8.4:15905925-15905944 | MS.gene060569:CDS | 25.0% |
| ! | AGAAGATTCAAAAGAACAAT+GGG | - | chr8.4:15905926-15905945 | MS.gene060569:CDS | 25.0% |
| ! | AGATGTACAAAATTTCAACA+AGG | - | chr8.4:15906467-15906486 | MS.gene060569:intron | 25.0% |
| ! | ATCGATGAACAATTATATGA+AGG | + | chr8.4:15906323-15906342 | None:intergenic | 25.0% |
| ! | ATGAAATATATTTGTGCACA+CGG | + | chr8.4:15906433-15906452 | None:intergenic | 25.0% |
| ! | GATATTACTAGTGTTAACAA+TGG | - | chr8.4:15906733-15906752 | MS.gene060569:CDS | 25.0% |
| ! | TCGATGAACAATTATATGAA+GGG | + | chr8.4:15906322-15906341 | None:intergenic | 25.0% |
| ! | TGTTAACACTAGTAATATCA+TGG | + | chr8.4:15906732-15906751 | None:intergenic | 25.0% |
| ! | TTACTTGGTTTAGACAAATT+TGG | - | chr8.4:15906577-15906596 | MS.gene060569:intron | 25.0% |
| ! | TTCAAACAAAGATTCTTCTA+AGG | - | chr8.4:15906091-15906110 | MS.gene060569:CDS | 25.0% |
| !!! | ATGCACAAAAGTATTTCATA+AGG | - | chr8.4:15906668-15906687 | MS.gene060569:CDS | 25.0% |
| ACAAATTTGGAAAAGGAGAT+TGG | - | chr8.4:15906590-15906609 | MS.gene060569:intron | 30.0% | |
| ACAATCCCTTCAAATACAAT+GGG | - | chr8.4:15906805-15906824 | MS.gene060569:CDS | 30.0% | |
| CAAGGATTCATTTCAATTCA+AGG | - | chr8.4:15906485-15906504 | MS.gene060569:intron | 30.0% | |
| CAATGAATAGAGATAGAAGA+AGG | - | chr8.4:15906698-15906717 | MS.gene060569:CDS | 30.0% | |
| CTAAACCCATTGTATTTGAA+GGG | + | chr8.4:15906813-15906832 | None:intergenic | 30.0% | |
| GGTTTAGACAAATTTGGAAA+AGG | - | chr8.4:15906583-15906602 | MS.gene060569:intron | 30.0% | |
| ! | ACTTGTTCCATACTTTTGTT+GGG | + | chr8.4:15905972-15905991 | None:intergenic | 30.0% |
| ! | TGATCTTTTATCTGAACTTG+TGG | + | chr8.4:15906120-15906139 | None:intergenic | 30.0% |
| !! | CAAGTTGTAATTTTGGAAGT+GGG | - | chr8.4:15906138-15906157 | MS.gene060569:CDS | 30.0% |
| !! | TCAAGTTGTAATTTTGGAAG+TGG | - | chr8.4:15906137-15906156 | MS.gene060569:CDS | 30.0% |
| !!! | TTCTTTTTCAATCCAATGCA+TGG | + | chr8.4:15905910-15905929 | None:intergenic | 30.0% |
| AAAGGAGAAAAGGAATTCCA+TGG | - | chr8.4:15906243-15906262 | MS.gene060569:intron | 35.0% | |
| AATCATCAAGCACCAATAAC+AGG | - | chr8.4:15906769-15906788 | MS.gene060569:CDS | 35.0% | |
| AGATGATGTTAGTGCAATTG+AGG | - | chr8.4:15906016-15906035 | MS.gene060569:CDS | 35.0% | |
| CAAGGAACTTTGTGATATCT+AGG | - | chr8.4:15906623-15906642 | MS.gene060569:CDS | 35.0% | |
| CACAATCCCTTCAAATACAA+TGG | - | chr8.4:15906804-15906823 | MS.gene060569:CDS | 35.0% | |
| CATCATCATCAGAACAAGAA+AGG | - | chr8.4:15906225-15906244 | MS.gene060569:CDS | 35.0% | |
| CCTAAACCCATTGTATTTGA+AGG | + | chr8.4:15906814-15906833 | None:intergenic | 35.0% | |
| GAAACAACATTACCAAGTGT+TGG | - | chr8.4:15905992-15906011 | MS.gene060569:CDS | 35.0% | |
| TCAGAACAAGAAAGGAGAAA+AGG | - | chr8.4:15906233-15906252 | MS.gene060569:intron | 35.0% | |
| TTACCATGTTGACCTGTTAT+TGG | + | chr8.4:15906784-15906803 | None:intergenic | 35.0% | |
| TTCTTCTTCATAGCTCCAAA+TGG | + | chr8.4:15905865-15905884 | None:intergenic | 35.0% | |
| ! | CACTTGTTCCATACTTTTGT+TGG | + | chr8.4:15905973-15905992 | None:intergenic | 35.0% |
| ! | CCTTCAAATACAATGGGTTT+AGG | - | chr8.4:15906811-15906830 | MS.gene060569:CDS | 35.0% |
| ! | CTTGTTCCATACTTTTGTTG+GGG | + | chr8.4:15905971-15905990 | None:intergenic | 35.0% |
| ! | GAGATTGGAGAAGTATATCA+AGG | - | chr8.4:15906605-15906624 | MS.gene060569:intron | 35.0% |
| ! | TAAGCAAGAGGTACAACATA+AGG | + | chr8.4:15906994-15907013 | None:intergenic | 35.0% |
| ! | TCTTTTATCTGAACTTGTGG+TGG | + | chr8.4:15906117-15906136 | None:intergenic | 35.0% |
| !! | AATTTTGGAAGTGGGTTTTC+TGG | - | chr8.4:15906146-15906165 | MS.gene060569:CDS | 35.0% |
| ACCTTTACCACTACTATGGT+GGG | + | chr8.4:15906189-15906208 | None:intergenic | 40.0% | |
| AGAATGCTATAGCCATGCAT+TGG | - | chr8.4:15905895-15905914 | MS.gene060569:CDS | 40.0% | |
| ATCCACCTTTACCACTACTA+TGG | + | chr8.4:15906193-15906212 | None:intergenic | 40.0% | |
| ATCTGTCCCCAACAAAAGTA+TGG | - | chr8.4:15905962-15905981 | MS.gene060569:CDS | 40.0% | |
| GATGTTAGTGCAATTGAGGA+AGG | - | chr8.4:15906020-15906039 | MS.gene060569:CDS | 40.0% | |
| TAACATCATCTGCCAACACT+TGG | + | chr8.4:15906007-15906026 | None:intergenic | 40.0% | |
| ! | ACATAAGGTGGATGATGATG+AGG | + | chr8.4:15906979-15906998 | None:intergenic | 40.0% |
| ! | TGGATGATGATGAGGATGAT+GGG | + | chr8.4:15906971-15906990 | None:intergenic | 40.0% |
| ACCCACCATAGTAGTGGTAA+AGG | - | chr8.4:15906185-15906204 | MS.gene060569:CDS | 45.0% | |
| CACCATAGTAGTGGTAAAGG+TGG | - | chr8.4:15906188-15906207 | MS.gene060569:CDS | 45.0% | |
| CACCTTTACCACTACTATGG+TGG | + | chr8.4:15906190-15906209 | None:intergenic | 45.0% | |
| CCATACATTCCTAAACCACC+AGG | + | chr8.4:15906874-15906893 | None:intergenic | 45.0% | |
| CCTGGTGGTTTAGGAATGTA+TGG | - | chr8.4:15906871-15906890 | MS.gene060569:CDS | 45.0% | |
| CTATGCTCTTCTTCTGTCCA+TGG | + | chr8.4:15906263-15906282 | None:intergenic | 45.0% | |
| CTCATATGGCATCTGCAGTT+GGG | - | chr8.4:15906929-15906948 | MS.gene060569:CDS | 45.0% | |
| GCACCAATAACAGGTCAACA+TGG | - | chr8.4:15906778-15906797 | MS.gene060569:CDS | 45.0% | |
| TACATTCCTAAACCACCAGG+AGG | + | chr8.4:15906871-15906890 | None:intergenic | 45.0% | |
| TGTACCTCTTGCTTACCCTA+TGG | - | chr8.4:15906999-15907018 | MS.gene060569:CDS | 45.0% | |
| TGTCATCAAGTGGAGCCATT+TGG | - | chr8.4:15905847-15905866 | MS.gene060569:CDS | 45.0% | |
| ! | CATGGACAGAAGAAGAGCAT+AGG | - | chr8.4:15906261-15906280 | MS.gene060569:intron | 45.0% |
| ! | GCAAGAGGTACAACATAAGG+TGG | + | chr8.4:15906991-15907010 | None:intergenic | 45.0% |
| ! | GGATGATGATGAGGATGATG+GGG | + | chr8.4:15906970-15906989 | None:intergenic | 45.0% |
| ! | GTGGATGATGATGAGGATGA+TGG | + | chr8.4:15906972-15906991 | None:intergenic | 45.0% |
| !! | GCATGACTTGCTACTTGTGT+TGG | + | chr8.4:15906652-15906671 | None:intergenic | 45.0% |
| !! | GTGGTAAAGGTGGATTGTCA+AGG | - | chr8.4:15906198-15906217 | MS.gene060569:CDS | 45.0% |
| !!! | GGAAGTGGGTTTTCTGGTTT+AGG | - | chr8.4:15906152-15906171 | MS.gene060569:CDS | 45.0% |
| AAACCACCAGGAGGATGATG+AGG | + | chr8.4:15906862-15906881 | None:intergenic | 50.0% | |
| ACTGCAGATGCCATATGAGC+AGG | + | chr8.4:15906928-15906947 | None:intergenic | 50.0% | |
| CATCATCCTCCTGGTGGTTT+AGG | - | chr8.4:15906862-15906881 | MS.gene060569:CDS | 50.0% | |
| GACTCTACCCACCATAGTAG+TGG | - | chr8.4:15906179-15906198 | MS.gene060569:CDS | 50.0% | |
| GCAACAACAGGATGTCCAAC+TGG | + | chr8.4:15906901-15906920 | None:intergenic | 50.0% | |
| GCTCATATGGCATCTGCAGT+TGG | - | chr8.4:15906928-15906947 | MS.gene060569:CDS | 50.0% | |
| TGTTGCTCCTCCTGCTCATA+TGG | - | chr8.4:15906915-15906934 | MS.gene060569:CDS | 50.0% | |
| ! | GGAATGTATGGAGCACCAGT+TGG | - | chr8.4:15906883-15906902 | MS.gene060569:CDS | 50.0% |
| !! | GCATTGTTGGAGGTGCCATA+GGG | + | chr8.4:15907017-15907036 | None:intergenic | 50.0% |
| !! | GGATGATGGGGAAGCATAAC+AGG | + | chr8.4:15906958-15906977 | None:intergenic | 50.0% |
| !! | TGCATTGTTGGAGGTGCCAT+AGG | + | chr8.4:15907018-15907037 | None:intergenic | 50.0% |
| GCAGATGCCATATGAGCAGG+AGG | + | chr8.4:15906925-15906944 | None:intergenic | 55.0% | |
| GTTCAGCCTCATCATCCTCC+TGG | - | chr8.4:15906853-15906872 | MS.gene060569:CDS | 55.0% | |
| TGAGCAGGAGGAGCAACAAC+AGG | + | chr8.4:15906913-15906932 | None:intergenic | 55.0% | |
| ! | GGTGCCATAGGGTAAGCAAG+AGG | + | chr8.4:15907006-15907025 | None:intergenic | 55.0% |
| CAGCCTCATCATCCTCCTGG+TGG | - | chr8.4:15906856-15906875 | MS.gene060569:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 15905846 | 15907044 | 15905846 | ID=MS.gene060569 |
| chr8.4 | mRNA | 15905846 | 15907044 | 15905846 | ID=MS.gene060569.t1;Parent=MS.gene060569 |
| chr8.4 | exon | 15906608 | 15907044 | 15906608 | ID=MS.gene060569.t1.exon1;Parent=MS.gene060569.t1 |
| chr8.4 | CDS | 15906608 | 15907044 | 15906608 | ID=cds.MS.gene060569.t1;Parent=MS.gene060569.t1 |
| chr8.4 | exon | 15905846 | 15906245 | 15905846 | ID=MS.gene060569.t1.exon2;Parent=MS.gene060569.t1 |
| chr8.4 | CDS | 15905846 | 15906245 | 15905846 | ID=cds.MS.gene060569.t1;Parent=MS.gene060569.t1 |
| Gene Sequence |
| Protein sequence |
