Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90141.t1 | XP_013464784.1 | 96.6 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 2.50E-39 | 171 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90141.t1 | A0A072V9V8 | 96.6 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 1.8e-39 | 171.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053108 | MS.gene90141 | 0.829104 | 6.20E-55 | -1.69E-46 |
| MS.gene053421 | MS.gene90141 | 0.808467 | 3.05E-50 | -1.69E-46 |
| MS.gene056618 | MS.gene90141 | 0.814751 | 1.31E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90141.t1 | MTR_2g481150 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 1.93e-60 | 179 |
| MS.gene90141.t1 | MTR_2g481160 | 93.258 | 89 | 6 | 0 | 1 | 89 | 1 | 89 | 9.80e-53 | 159 |
| MS.gene90141.t1 | MTR_2g081155 | 74.725 | 91 | 17 | 4 | 1 | 89 | 1 | 87 | 7.87e-41 | 129 |
| MS.gene90141.t1 | MTR_4g045990 | 56.180 | 89 | 35 | 1 | 1 | 85 | 1 | 89 | 3.72e-31 | 105 |
| MS.gene90141.t1 | MTR_2g089945 | 53.933 | 89 | 34 | 2 | 2 | 85 | 5 | 91 | 5.14e-28 | 97.4 |
| MS.gene90141.t1 | MTR_2g089905 | 55.914 | 93 | 32 | 3 | 1 | 85 | 1 | 92 | 1.97e-27 | 95.9 |
| MS.gene90141.t1 | MTR_2g089930 | 51.724 | 87 | 39 | 1 | 2 | 85 | 5 | 91 | 1.18e-26 | 94.0 |
| MS.gene90141.t1 | MTR_2g089940 | 52.174 | 92 | 36 | 3 | 1 | 85 | 1 | 91 | 4.43e-26 | 92.4 |
| MS.gene90141.t1 | MTR_2g089985 | 52.174 | 92 | 36 | 3 | 1 | 85 | 1 | 91 | 4.43e-26 | 92.4 |
| MS.gene90141.t1 | MTR_2g089960 | 52.174 | 92 | 36 | 3 | 1 | 85 | 1 | 91 | 4.43e-26 | 92.4 |
| MS.gene90141.t1 | MTR_2g089970 | 51.685 | 89 | 37 | 3 | 1 | 84 | 1 | 88 | 5.12e-25 | 89.7 |
| MS.gene90141.t1 | MTR_2g089990 | 50.562 | 89 | 38 | 3 | 1 | 84 | 1 | 88 | 4.98e-24 | 87.0 |
| MS.gene90141.t1 | MTR_2g089965 | 52.577 | 97 | 33 | 3 | 1 | 85 | 1 | 96 | 5.09e-24 | 87.4 |
| MS.gene90141.t1 | MTR_2g089950 | 50.000 | 86 | 38 | 3 | 1 | 84 | 1 | 83 | 5.16e-22 | 82.0 |
| MS.gene90141.t1 | MTR_2g089865 | 54.321 | 81 | 30 | 2 | 2 | 77 | 5 | 83 | 8.01e-21 | 84.0 |
| MS.gene90141.t1 | MTR_2g089925 | 46.512 | 86 | 41 | 3 | 1 | 84 | 1 | 83 | 4.28e-20 | 77.0 |
| MS.gene90141.t1 | MTR_2g089835 | 42.697 | 89 | 46 | 3 | 1 | 85 | 1 | 88 | 1.64e-15 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90141.t1 | AT4G10265 | 65.882 | 85 | 27 | 2 | 1 | 85 | 1 | 83 | 3.29e-34 | 112 |
| MS.gene90141.t1 | AT4G10270 | 53.333 | 90 | 37 | 1 | 1 | 85 | 1 | 90 | 1.10e-27 | 96.7 |
| MS.gene90141.t1 | AT4G33560 | 40.625 | 96 | 49 | 3 | 1 | 89 | 1 | 95 | 3.89e-15 | 64.7 |
Find 22 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGTCCATGACTTTCTTCA+TGG | 0.260032 | 2.3:-17294000 | None:intergenic |
| CTTGGTTCTCTTGACTTTGT+TGG | 0.278878 | 2.3:-17293970 | None:intergenic |
| AGAAGCAGATGACAATTTCT+TGG | 0.287978 | 2.3:-17293934 | None:intergenic |
| GTATGATCTGATATTGTTCT+TGG | 0.335862 | 2.3:-17293904 | None:intergenic |
| AAGACCAACTTGGTGTTTGC+AGG | 0.345102 | 2.3:+17293847 | MS.gene90141:CDS |
| TTTCTTCATGGACTCTTCCT+TGG | 0.358781 | 2.3:-17293988 | None:intergenic |
| CATTAAAACCTTGCAGTACT+AGG | 0.414275 | 2.3:-17294035 | None:intergenic |
| AAGTCATGGACTTGAATTGC+TGG | 0.419101 | 2.3:+17294009 | MS.gene90141:CDS |
| TGAGTGCAGCAACAAAAGCT+TGG | 0.493392 | 2.3:+17293787 | MS.gene90141:CDS |
| GAGTGCAGCAACAAAAGCTT+GGG | 0.515342 | 2.3:+17293788 | MS.gene90141:CDS |
| AGCATCAAGCATAGGAGCAG+TGG | 0.536575 | 2.3:+17293815 | MS.gene90141:CDS |
| AGTCATGGACTTGAATTGCT+GGG | 0.546309 | 2.3:+17294010 | MS.gene90141:CDS |
| ATCAAGCATAGGAGCAGTGG+AGG | 0.556011 | 2.3:+17293818 | MS.gene90141:CDS |
| ACCAACTTGGTGTTTGCAGG+TGG | 0.578723 | 2.3:+17293850 | MS.gene90141:CDS |
| TGGGTAGTAGCATCAAGCAT+AGG | 0.606039 | 2.3:+17293807 | MS.gene90141:CDS |
| GCTGGGGTCCTAGTACTGCA+AGG | 0.615045 | 2.3:+17294027 | MS.gene90141:CDS |
| TCCACCTGCAAACACCAAGT+TGG | 0.616927 | 2.3:-17293851 | None:intergenic |
| AGAGTCCATGAAGAAAGTCA+TGG | 0.627949 | 2.3:+17293995 | MS.gene90141:CDS |
| GTCATGGACTTGAATTGCTG+GGG | 0.644611 | 2.3:+17294011 | MS.gene90141:CDS |
| GAGGCCTTAAAAGACCAACT+TGG | 0.665081 | 2.3:+17293837 | MS.gene90141:CDS |
| GATGCAGTGATCTAAAAGCA+TGG | 0.715842 | 2.3:-17293875 | None:intergenic |
| CAACAAAGTCAAGAGAACCA+AGG | 0.721950 | 2.3:+17293971 | MS.gene90141:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTATGATCTGATATTGTTCT+TGG | - | chr2.3:17293907-17293926 | None:intergenic | 30.0% | |
| AGAAGCAGATGACAATTTCT+TGG | - | chr2.3:17293937-17293956 | None:intergenic | 35.0% | |
| !!! | AACACCAAGTTGGTCTTTTA+AGG | - | chr2.3:17293844-17293863 | None:intergenic | 35.0% |
| AAGTCATGGACTTGAATTGC+TGG | + | chr2.3:17294009-17294028 | MS.gene90141:CDS | 40.0% | |
| AGAGTCCATGAAGAAAGTCA+TGG | + | chr2.3:17293995-17294014 | MS.gene90141:CDS | 40.0% | |
| AGTCATGGACTTGAATTGCT+GGG | + | chr2.3:17294010-17294029 | MS.gene90141:CDS | 40.0% | |
| CAACAAAGTCAAGAGAACCA+AGG | + | chr2.3:17293971-17293990 | MS.gene90141:CDS | 40.0% | |
| CAAGTCCATGACTTTCTTCA+TGG | - | chr2.3:17294003-17294022 | None:intergenic | 40.0% | |
| GATGCAGTGATCTAAAAGCA+TGG | - | chr2.3:17293878-17293897 | None:intergenic | 40.0% | |
| TTTCTTCATGGACTCTTCCT+TGG | - | chr2.3:17293991-17294010 | None:intergenic | 40.0% | |
| ! | CTTGGTTCTCTTGACTTTGT+TGG | - | chr2.3:17293973-17293992 | None:intergenic | 40.0% |
| GAGGCCTTAAAAGACCAACT+TGG | + | chr2.3:17293837-17293856 | MS.gene90141:CDS | 45.0% | |
| GAGTGCAGCAACAAAAGCTT+GGG | + | chr2.3:17293788-17293807 | MS.gene90141:CDS | 45.0% | |
| GTCATGGACTTGAATTGCTG+GGG | + | chr2.3:17294011-17294030 | MS.gene90141:CDS | 45.0% | |
| TGAGTGCAGCAACAAAAGCT+TGG | + | chr2.3:17293787-17293806 | MS.gene90141:CDS | 45.0% | |
| TGGGTAGTAGCATCAAGCAT+AGG | + | chr2.3:17293807-17293826 | MS.gene90141:CDS | 45.0% | |
| !! | AAGACCAACTTGGTGTTTGC+AGG | + | chr2.3:17293847-17293866 | MS.gene90141:CDS | 45.0% |
| AGCATCAAGCATAGGAGCAG+TGG | + | chr2.3:17293815-17293834 | MS.gene90141:CDS | 50.0% | |
| ATCAAGCATAGGAGCAGTGG+AGG | + | chr2.3:17293818-17293837 | MS.gene90141:CDS | 50.0% | |
| TCCACCTGCAAACACCAAGT+TGG | - | chr2.3:17293854-17293873 | None:intergenic | 50.0% | |
| !! | ACCAACTTGGTGTTTGCAGG+TGG | + | chr2.3:17293850-17293869 | MS.gene90141:CDS | 50.0% |
| GCTGGGGTCCTAGTACTGCA+AGG | + | chr2.3:17294027-17294046 | MS.gene90141:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 17293786 | 17294055 | 17293786 | ID=MS.gene90141 |
| chr2.3 | mRNA | 17293786 | 17294055 | 17293786 | ID=MS.gene90141.t1;Parent=MS.gene90141 |
| chr2.3 | exon | 17293786 | 17294055 | 17293786 | ID=MS.gene90141.t1.exon1;Parent=MS.gene90141.t1 |
| chr2.3 | CDS | 17293786 | 17294055 | 17293786 | ID=cds.MS.gene90141.t1;Parent=MS.gene90141.t1 |
| Gene Sequence |
| Protein sequence |
